pycom07g24650

Lecithin retinol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002037
Physical Location & Seq
Reverse (-)
25274168 .. 25275274
1107 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 780 bp
ATGGGACTGTTCTCAAACAAGGTTGATAAGTCCCAGATCAAGCCAGGAGATCACGTCTATACCTATAGAGGGCTGCACACATACTCCCACCATGGAATATGCGTGGAGGCAGATAGGGTGATCCACTACACCAGAACACAAGCTGCAAAAAAGAAATTAGGGATGTTTCGGAAGACTAAATGCAAAAACTGCGGGCATCACCCAGATATGGCACGAGGATCGATCAAAACTTGCGTCGACTGCTTCCTACACGGCCATCGGCTTTACCGCTTCGGATACGGAGTCTCTTATGCTCACTTCCTATTGAAATTTCCAGGAACTTGCACGACGGGTCGTTCCAATTCAGCCGACGAAGTCATAGCTCGCGCCACAGAACTGCTCAACAAGGAAGAGGGTTTCGGGGACTATAAGCTGTTTAGTAACAACTGCGAATCGTTTGCGATTTTCTGCAAAACAGGAAAACGTCTGAGCGAGCAGGCATTTTCTGCCTTAAATAGTACTAAAATCTTGTTCAAAGCGTTTGTGAAGCATAACGTGGAAAGGCTTCTGGAAGACGTGTCTATTCATCAACCAGACAAGTACATGCTGTTGAAACAAACTTTTGAAACTGATGTTAACTTGCCCAGCAAGGAAATTATAGCCCAACTTCGAAAGCTCAACGAGAAATATGATGACGAGCAGGAGGATGTCACTGATGAAGATCTGTTAGACGACCACGAGTGTCTAGAGATCAACGATGAGGAGGGGGATGATGGCCGTCCCTCTCAATCATATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRAT

Protein Analysis

260

Amino Acids

29.66

Weight (kDa)

6.35

Isoelectric Point (pI)

32.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRAT PF04970 12 - 154 3.5e-22 Lecithin retinol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 237
AccII CGCG 1 cut(s) 366
AciI CCGC 2 cut(s) 192, 268
AclWI GGATC 2 cut(s) 115, 226
AcoI YGGCCR 2 cut(s) 253, 754
AcsI RAATTY 1 cut(s) 308
AfaI GTAC 2 cut(s) 499, 581
AfiI CCNNNNNNNGG 2 cut(s) 69, 208
AflIII ACRYGT 1 cut(s) 555
AgsI TTSAA 4 cut(s) 307, 514, 592, 605
AjiI CACGTC 2 cut(s) 55, 556
AjnI CCWGG 2 cut(s) 43, 313
AluBI AGCT 4 cut(s) 143, 362, 412, 655
AluI AGCT 4 cut(s) 143, 362, 412, 655
Alw26I GTCTC 1 cut(s) 289
AlwI GGATC 2 cut(s) 115, 226
AoxI GGCC 2 cut(s) 253, 754
ApeKI GCWGC 2 cut(s) 73, 143
ApoI RAATTY 1 cut(s) 308
ArsI GACNNNNNNTTYG 2 cut(s) 219, 251
Asp700I GAANNNNTTC 1 cut(s) 543
AspLEI GCGC 1 cut(s) 368
AsuHPI GGTGA 2 cut(s) 130, 191
AsuII TTCGAA 1 cut(s) 649
BauI CACGAG 2 cut(s) 213, 716
BbsI GAAGAC 2 cut(s) 179, 558
BbvI GCAGC 2 cut(s) 60, 130
BccI CCATC 2 cut(s) 264, 746
BceAI ACGGC 2 cut(s) 268, 741
BcgI CGANNNNNNTGC 4 cut(s) 201, 235, 419, 453
BciT130I CCWGG 2 cut(s) 45, 315
BciVI GTATCC 1 cut(s) 269
BcoDI GTCTC 1 cut(s) 289
BfaI CTAG 1 cut(s) 725
BfmI CTRYAG 1 cut(s) 64
BfuI GTATCC 1 cut(s) 269
BglII AGATCT 1 cut(s) 700
BisI GCNGC 2 cut(s) 74, 144
BlsI GCNGC 2 cut(s) 75, 145
BmcAI AGTACT 1 cut(s) 499
Bme1390I CCNGG 2 cut(s) 45, 315
BmgBI CACGTC 2 cut(s) 55, 556
BmrFI CCNGG 2 cut(s) 45, 315
BmsI GCATC 1 cut(s) 205
BpiI GAAGAC 2 cut(s) 179, 558
Bpu14I TTCGAA 1 cut(s) 649
Bsa29I ATCGAT 1 cut(s) 221
BsaBI GATNNNNATC 1 cut(s) 699
BsaJI CCNNGG 1 cut(s) 91
BsaXI ACNNNNNCTCC 4 cut(s) 39, 68, 69, 98
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 208
Bse8I GATNNNNATC 1 cut(s) 699
BseBI CCWGG 2 cut(s) 45, 315
BseCI ATCGAT 1 cut(s) 221
BseDI CCNNGG 1 cut(s) 91
BseGI GGATG 3 cut(s) 168, 691, 754
BseJI GATNNNNATC 1 cut(s) 699
BseLI CCNNNNNNNGG 2 cut(s) 69, 208
BseMII CTCAG 1 cut(s) 458
BseRI GAGGAG 1 cut(s) 755
BseXI GCAGC 2 cut(s) 60, 130
BseYI CCCAGC 1 cut(s) 623
BsgI GTGCAG 1 cut(s) 59
Bsh1236I CGCG 1 cut(s) 366
BshFI GGCC 2 cut(s) 255, 756
BshVI ATCGAT 1 cut(s) 221
BslFI GGGAC 4 cut(s) 16, 18, 416, 744
BslI CCNNNNNNNGG 2 cut(s) 69, 208
BsmAI GTCTC 1 cut(s) 289
BsmFI GGGAC 4 cut(s) 16, 18, 416, 744
BsnI GGCC 2 cut(s) 255, 756
Bsp119I TTCGAA 1 cut(s) 649
Bsp143I GATC 7 cut(s) 36, 49, 120, 218, 222, 700, 729
Bsp19I CCATGG 1 cut(s) 91
BspACI CCGC 2 cut(s) 192, 268
BspANI GGCC 2 cut(s) 255, 756
BspCNI CTCAG 1 cut(s) 459
BspDI ATCGAT 1 cut(s) 221
BspFNI CGCG 1 cut(s) 366
BspPI GGATC 2 cut(s) 115, 226
BspT104I TTCGAA 1 cut(s) 649
BssECI CCNNGG 1 cut(s) 91
BssMI GATC 7 cut(s) 36, 49, 120, 218, 222, 700, 729
BssSI CACGAG 2 cut(s) 213, 716
BssT1I CCWWGG 1 cut(s) 91
Bst2BI CACGAG 2 cut(s) 213, 716
Bst2UI CCWGG 2 cut(s) 45, 315
Bst4CI ACNGT 1 cut(s) 9
Bst6I CTCTTC 1 cut(s) 384
BstAPI GCANNNNNTGC 2 cut(s) 189, 485
BstBI TTCGAA 1 cut(s) 649
BstC8I GCNNGC 4 cut(s) 194, 364, 473, 477
BstDEI CTNAG 1 cut(s) 467
BstDSI CCRYGG 1 cut(s) 91
BstF5I GGATG 3 cut(s) 168, 691, 754
BstFNI CGCG 1 cut(s) 366
BstHHI GCGC 1 cut(s) 368
BstKTI GATC 7 cut(s) 39, 52, 123, 221, 225, 703, 732
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 7 cut(s) 36, 49, 120, 218, 222, 700, 729
BstMWI GCNNNNNNNGC 3 cut(s) 189, 240, 485
BstNI CCWGG 2 cut(s) 45, 315
BstNSI RCATGY 1 cut(s) 586
BstSCI CCNGG 2 cut(s) 43, 313
BstSFI CTRYAG 1 cut(s) 64
BstUI CGCG 1 cut(s) 366
BstV1I GCAGC 2 cut(s) 60, 130
BstV2I GAAGAC 2 cut(s) 179, 558
BstX2I RGATCY 1 cut(s) 700
BstYI RGATCY 1 cut(s) 700
Bsu15I ATCGAT 1 cut(s) 221
BsuI GTATCC 1 cut(s) 269
BsuRI GGCC 2 cut(s) 255, 756
BsuTUI ATCGAT 1 cut(s) 221
BtgI CCRYGG 1 cut(s) 91
BtrI CACGTC 2 cut(s) 55, 556
BtsCI GGATG 3 cut(s) 168, 691, 754
BtsIMutI CAGTG 1 cut(s) 690
Cac8I GCNNGC 4 cut(s) 194, 364, 473, 477
CfoI GCGC 1 cut(s) 368
ClaI ATCGAT 1 cut(s) 221
CseI GACGC 1 cut(s) 223
Csp6I GTAC 2 cut(s) 498, 580
CviAII CATG 2 cut(s) 92, 583
CviQI GTAC 2 cut(s) 498, 580
DdeI CTNAG 1 cut(s) 467
DpnI GATC 7 cut(s) 38, 51, 122, 220, 224, 702, 731
DpnII GATC 7 cut(s) 36, 49, 120, 218, 222, 700, 729
EaeI YGGCCR 2 cut(s) 253, 754
Eam1104I CTCTTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 384
Eco130I CCWWGG 1 cut(s) 91
EcoRII CCWGG 2 cut(s) 43, 313
EcoT14I CCWWGG 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 91
FaeI CATG 2 cut(s) 95, 586
FaqI GGGAC 4 cut(s) 16, 18, 416, 744
FatI CATG 2 cut(s) 91, 582
FauI CCCGC 1 cut(s) 185
FauNDI CATATG 1 cut(s) 772
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 2 cut(s) 74, 144
FokI GGATG 3 cut(s) 175, 698, 761
Fsp4HI GCNGC 2 cut(s) 74, 144
FspBI CTAG 1 cut(s) 725
GlaI GCGC 1 cut(s) 367
GluI GCNGC 2 cut(s) 74, 144
GsaI CCCAGC 1 cut(s) 627
HaeIII GGCC 2 cut(s) 255, 756
HgaI GACGC 1 cut(s) 223
HhaI GCGC 1 cut(s) 368
Hin1II CATG 2 cut(s) 95, 586
Hin6I GCGC 1 cut(s) 366
HinP1I GCGC 1 cut(s) 366
HincII GTYRAC 2 cut(s) 238, 616
HindII GTYRAC 2 cut(s) 238, 616
HinfI GANTC 2 cut(s) 282, 431
HpaI GTTAAC 1 cut(s) 616
HphI GGTGA 2 cut(s) 130, 191
Hpy166II GTNNAC 2 cut(s) 238, 616
Hpy188I TCNGA 3 cut(s) 171, 275, 468
Hpy188III TCNNGA 2 cut(s) 548, 725
Hpy8I GTNNAC 2 cut(s) 238, 616
Hpy99I CGWCG 3 cut(s) 239, 331, 353
HpyCH4III ACNGT 1 cut(s) 9
HpyCH4IV ACGT 4 cut(s) 54, 463, 534, 555
HpyCH4V TGCA 5 cut(s) 76, 146, 183, 324, 450
HpyF10VI GCNNNNNNNGC 3 cut(s) 189, 240, 485
HpyF3I CTNAG 1 cut(s) 467
HpySE526I ACGT 4 cut(s) 54, 463, 534, 555
Hsp92II CATG 2 cut(s) 95, 586
HspAI GCGC 1 cut(s) 366
KspAI GTTAAC 1 cut(s) 616
Kzo9I GATC 7 cut(s) 36, 49, 120, 218, 222, 700, 729
Lsp1109I GCAGC 2 cut(s) 60, 130
LweI GCATC 1 cut(s) 205
MaeI CTAG 1 cut(s) 725
MaeII ACGT 4 cut(s) 54, 463, 534, 555
MaeIII GTNAC 2 cut(s) 419, 688
MalI GATC 7 cut(s) 38, 51, 122, 220, 224, 702, 731
MboI GATC 7 cut(s) 36, 49, 120, 218, 222, 700, 729
MboII GAAGA 4 cut(s) 184, 401, 563, 710
MflI RGATCY 1 cut(s) 700
MluCI AATT 4 cut(s) 155, 308, 340, 633
MlyI GAGTC 1 cut(s) 291
MnlI CCTC 8 cut(s) 62, 100, 209, 385, 676, 733, 736, 772
MroXI GAANNNNTTC 1 cut(s) 543
MseI TTAA 3 cut(s) 491, 615, 778
MspR9I CCNGG 2 cut(s) 45, 315
MvaI CCWGG 2 cut(s) 45, 315
MvnI CGCG 1 cut(s) 366
MwoI GCNNNNNNNGC 3 cut(s) 189, 240, 485
NcoI CCATGG 1 cut(s) 91
NdeI CATATG 1 cut(s) 772
NdeII GATC 7 cut(s) 36, 49, 120, 218, 222, 700, 729
NlaIII CATG 2 cut(s) 95, 586
NmuCI GTSAC 1 cut(s) 688
NspI RCATGY 1 cut(s) 586
NspV TTCGAA 1 cut(s) 649
PdmI GAANNNNTTC 1 cut(s) 543
PfeI GAWTC 1 cut(s) 431
PflFI GACNNNGTC 1 cut(s) 353
PfoI TCCNGGA 1 cut(s) 313
PkrI GCNGC 2 cut(s) 75, 145
PleI GAGTC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 290
Psp6I CCWGG 2 cut(s) 43, 313
PspFI CCCAGC 1 cut(s) 623
PspGI CCWGG 2 cut(s) 43, 313
PsuI RGATCY 1 cut(s) 700
PsyI GACNNNGTC 1 cut(s) 353
RsaI GTAC 2 cut(s) 499, 581
RsaNI GTAC 2 cut(s) 498, 580
SalI GTCGAC 1 cut(s) 236
SaqAI TTAA 3 cut(s) 491, 615, 778
SatI GCNGC 2 cut(s) 74, 144
Sau3AI GATC 7 cut(s) 36, 49, 120, 218, 222, 700, 729
ScaI AGTACT 1 cut(s) 499
SchI GAGTC 1 cut(s) 291
ScrFI CCNGG 2 cut(s) 45, 315
SfaNI GCATC 1 cut(s) 205
SfcI CTRYAG 1 cut(s) 64
SfuI TTCGAA 1 cut(s) 649
Sse9I AATT 4 cut(s) 155, 308, 340, 633
SsiI CCGC 2 cut(s) 192, 268
SspMI CTAG 1 cut(s) 725
StyD4I CCNGG 2 cut(s) 43, 313
StyI CCWWGG 1 cut(s) 91
TaaI ACNGT 1 cut(s) 9
TaiI ACGT 4 cut(s) 57, 466, 537, 558
TaqI TCGA 3 cut(s) 221, 237, 649
TasI AATT 4 cut(s) 155, 308, 340, 633
TatI WGTACW 2 cut(s) 497, 579
TfiI GAWTC 1 cut(s) 431
Tru1I TTAA 3 cut(s) 491, 615, 778
Tru9I TTAA 3 cut(s) 491, 615, 778
TscAI CASTG 1 cut(s) 697
TseFI GTSAC 1 cut(s) 688
TseI GCWGC 2 cut(s) 73, 143
Tsp45I GTSAC 1 cut(s) 688
TspDTI ATGAA 2 cut(s) 554, 711
TspGWI ACGGA 1 cut(s) 294
TspRI CASTG 1 cut(s) 697
Tth111I GACNNNGTC 1 cut(s) 353
XapI RAATTY 1 cut(s) 308
XbaI TCTAGA 1 cut(s) 724
XceI RCATGY 1 cut(s) 586
XmiI GTMKAC 1 cut(s) 237
XmnI GAANNNNTTC 1 cut(s) 543
XspI CTAG 1 cut(s) 725
ZrmI AGTACT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.