pycom09g03770

HAUS augmin-like complex subunit 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0003213
Physical Location & Seq
Forward (+)
2783604 .. 2785945
2342 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 888 bp
ATGTCGATGGGGACTGACAGCACTTGGGTTGGGAAGAAACCGCTGCGGCGCATCGGTGGCATGTCCGACGCCCTCTCCATCGCTTCGGATCTCGGCTTCTCCGTTGCCGCTCCTCCTACTCAGGAAGAGCTTCAGAACTTGTCCAGTAGTACTGGTGAAAAGGGTGATGACTTGATAAAGGTTCTAAGGGACCTTACCTCTGTACAGAGAAAAATAACCGATCTGCAAGTTGAACTCCAAGGGAGAAAGGATGATAAAAACGTAGCACATTTGACACATGCGAGTGAAATGGAGAAGAAGTGCGAAACTTTAGCAAGGATCACTACCATTTTGAAAGATGTGATTCAAAATAAGGATCGCATCATAGCCCGTCTTCAACAACCATATTCACTCGATTGCATTCCAGTAGAAGCAGAGTATCAGAAACAGTTCTCTGAATTATTGATGAAGGCTGCTGGTGATTATGGGGCCTTGACAGCATCAGTTGCAGATTTCCAGTGGAGTCAGACATTTAAGGAGCCTCCTTCTGTTTGGGGGGAAATGCTTCGTCCAATTCCTGTGGCCTTAGCATCATGCACCCGGTTCTTTGAAGCAATGTCTGCCATGAGGGAATCATTTTCAACACTTCAGCATCTCAGAGTTGGTCATACTGATGCGTCATCACCTATAACACCAGGCAAGGATTCTTCTCGAAGAATACCAGGTGATTCCGAATGCGTAACTCCACCTCCTTGGAGAAGTGAGCCAAGCTTTGATGACTTAGCTATGAGAAGCTCAAGAAAGCCTGAAGTCGAGCATCAAGAATCAGAGGATGAAAACAGTGGGGTGGATGGAGCAAGCCACAGGAGATTGTCATGGCCTCCATCAGTTAAAAACAACGGTGTTTAG

Protein Analysis

296

Amino Acids

32.64

Weight (kDa)

5.62

Isoelectric Point (pI)

54.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS2 PF15003 19 - 208 1.2e-89 HAUS augmin-like complex subunit 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 110
AciI CCGC 3 cut(s) 41, 46, 108
AclWI GGATC 3 cut(s) 96, 326, 363
AcuI CTGAAG 3 cut(s) 116, 611, 807
AcyI GRCGYC 1 cut(s) 69
AfaI GTAC 2 cut(s) 151, 204
AgsI TTSAA 6 cut(s) 233, 334, 347, 377, 590, 621
AjnI CCWGG 2 cut(s) 673, 700
AluBI AGCT 4 cut(s) 130, 750, 764, 774
AluI AGCT 4 cut(s) 130, 750, 764, 774
AlwI GGATC 3 cut(s) 96, 326, 363
AoxI GGCC 3 cut(s) 468, 561, 857
ApeKI GCWGC 2 cut(s) 43, 452
Asp700I GAANNNNTTC 3 cut(s) 129, 428, 543
AspLEI GCGC 1 cut(s) 51
AspS9I GGNCC 2 cut(s) 190, 468
AsuC2I CCSGG 1 cut(s) 580
AsuHPI GGTGA 5 cut(s) 167, 176, 470, 654, 716
AvaII GGWCC 1 cut(s) 190
BbsI GAAGAC 1 cut(s) 365
BbvI GCAGC 2 cut(s) 30, 439
BccI CCATC 3 cut(s) 86, 824, 871
BciT130I CCWGG 2 cut(s) 675, 702
BcnI CCSGG 1 cut(s) 580
BisI GCNGC 4 cut(s) 44, 47, 108, 453
BlsI GCNGC 4 cut(s) 45, 48, 109, 454
BmcAI AGTACT 1 cut(s) 151
Bme1390I CCNGG 3 cut(s) 580, 675, 702
Bme18I GGWCC 1 cut(s) 190
BmgT120I GGNCC 2 cut(s) 190, 468
BmiI GGNNCC 3 cut(s) 191, 469, 519
BmrFI CCNGG 3 cut(s) 580, 675, 702
BmsI GCATC 7 cut(s) 60, 369, 488, 578, 640, 643, 805
BpiI GAAGAC 1 cut(s) 365
Bpu10I CCTNAGC 1 cut(s) 565
BpuEI CTTGAG 1 cut(s) 760
BpuMI CCSGG 1 cut(s) 580
BsaHI GRCGYC 1 cut(s) 69
BsaJI CCNNGG 2 cut(s) 238, 731
BsaXI ACNNNNNCTCC 6 cut(s) 59, 89, 284, 314, 712, 742
Bse1I ACTGG 4 cut(s) 144, 157, 404, 496
Bse3DI GCAATG 1 cut(s) 600
BseBI CCWGG 2 cut(s) 675, 702
BseDI CCNNGG 2 cut(s) 238, 731
BseGI GGATG 3 cut(s) 256, 817, 835
BseMI GCAATG 1 cut(s) 600
BseMII CTCAG 2 cut(s) 134, 649
BseNI ACTGG 4 cut(s) 144, 157, 404, 496
BseRI GAGGAG 1 cut(s) 102
BseXI GCAGC 2 cut(s) 30, 439
BshFI GGCC 3 cut(s) 470, 563, 859
BsiSI CCGG 1 cut(s) 580
BslFI GGGAC 2 cut(s) 25, 203
BsmFI GGGAC 2 cut(s) 25, 203
BsmI GAATGC 2 cut(s) 399, 719
BsnI GGCC 3 cut(s) 470, 563, 859
Bsp1407I TGTACA 1 cut(s) 202
Bsp143I GATC 4 cut(s) 88, 220, 318, 355
BspACI CCGC 3 cut(s) 41, 46, 108
BspANI GGCC 3 cut(s) 470, 563, 859
BspCNI CTCAG 2 cut(s) 133, 648
BspLI GGNNCC 3 cut(s) 191, 469, 519
BspPI GGATC 3 cut(s) 96, 326, 363
BspQI GCTCTTC 1 cut(s) 120
BsrBI CCGCTC 1 cut(s) 110
BsrDI GCAATG 1 cut(s) 600
BsrGI TGTACA 1 cut(s) 202
BsrI ACTGG 4 cut(s) 144, 157, 404, 496
BssECI CCNNGG 2 cut(s) 238, 731
BssMI GATC 4 cut(s) 88, 220, 318, 355
BssNI GRCGYC 1 cut(s) 69
BssT1I CCWWGG 2 cut(s) 238, 731
Bst2UI CCWGG 2 cut(s) 675, 702
Bst4CI ACNGT 3 cut(s) 429, 821, 881
Bst6I CTCTTC 1 cut(s) 120
BstACI GRCGYC 1 cut(s) 69
BstAPI GCANNNNNTGC 2 cut(s) 485, 599
BstAUI TGTACA 1 cut(s) 202
BstC8I GCNNGC 1 cut(s) 838
BstDEI CTNAG 5 cut(s) 120, 185, 565, 635, 760
BstF5I GGATG 3 cut(s) 256, 817, 835
BstHHI GCGC 1 cut(s) 51
BstKTI GATC 4 cut(s) 91, 223, 321, 358
BstMBI GATC 4 cut(s) 88, 220, 318, 355
BstMWI GCNNNNNNNGC 4 cut(s) 57, 476, 485, 599
BstNI CCWGG 2 cut(s) 675, 702
BstNSI RCATGY 2 cut(s) 64, 281
BstSCI CCNGG 3 cut(s) 578, 673, 700
BstV1I GCAGC 2 cut(s) 30, 439
BstV2I GAAGAC 1 cut(s) 365
BstX2I RGATCY 1 cut(s) 88
BstXI CCANNNNNNTGG 1 cut(s) 732
BstYI RGATCY 1 cut(s) 88
BsuRI GGCC 3 cut(s) 470, 563, 859
BtgZI GCGATG 1 cut(s) 64
BtsCI GGATG 3 cut(s) 256, 817, 835
BtsIMutI CAGTG 2 cut(s) 503, 826
Cac8I GCNNGC 1 cut(s) 838
CfoI GCGC 1 cut(s) 51
Cfr13I GGNCC 2 cut(s) 190, 468
CseI GACGC 2 cut(s) 77, 645
CsiI ACCWGGT 1 cut(s) 700
Csp6I GTAC 2 cut(s) 150, 203
CspCI CAANNNNNGTGG 2 cut(s) 540, 575
CviAII CATG 5 cut(s) 61, 278, 573, 604, 855
CviQI GTAC 2 cut(s) 150, 203
DdeI CTNAG 5 cut(s) 120, 185, 565, 635, 760
DpnI GATC 4 cut(s) 90, 222, 320, 357
DpnII GATC 4 cut(s) 88, 220, 318, 355
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
Eco130I CCWWGG 2 cut(s) 238, 731
Eco47I GGWCC 1 cut(s) 190
Eco57I CTGAAG 3 cut(s) 116, 611, 807
EcoO109I RGGNCCY 2 cut(s) 190, 468
EcoRII CCWGG 2 cut(s) 673, 700
EcoT14I CCWWGG 2 cut(s) 238, 731
ErhI CCWWGG 2 cut(s) 238, 731
FaeI CATG 5 cut(s) 64, 281, 576, 607, 858
FaqI GGGAC 2 cut(s) 25, 203
FatI CATG 5 cut(s) 60, 277, 572, 603, 854
Fnu4HI GCNGC 4 cut(s) 44, 47, 108, 453
FokI GGATG 3 cut(s) 263, 824, 842
Fsp4HI GCNGC 4 cut(s) 44, 47, 108, 453
GlaI GCGC 1 cut(s) 50
GluI GCNGC 4 cut(s) 44, 47, 108, 453
HaeIII GGCC 3 cut(s) 470, 563, 859
HapII CCGG 1 cut(s) 580
HgaI GACGC 2 cut(s) 77, 645
HhaI GCGC 1 cut(s) 51
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 5 cut(s) 64, 281, 576, 607, 858
Hin6I GCGC 1 cut(s) 49
HinP1I GCGC 1 cut(s) 49
HindIII AAGCTT 1 cut(s) 748
HinfI GANTC 6 cut(s) 343, 502, 611, 683, 707, 803
HpaII CCGG 1 cut(s) 580
HphI GGTGA 5 cut(s) 167, 176, 470, 654, 716
Hpy188I TCNGA 9 cut(s) 67, 88, 135, 423, 436, 507, 638, 712, 808
Hpy188III TCNNGA 4 cut(s) 122, 690, 777, 800
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 2 cut(s) 442, 534
HpyCH4III ACNGT 3 cut(s) 429, 821, 881
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 4 cut(s) 226, 399, 488, 576
HpyF10VI GCNNNNNNNGC 4 cut(s) 57, 476, 485, 599
HpyF3I CTNAG 5 cut(s) 120, 185, 565, 635, 760
HpySE526I ACGT 1 cut(s) 261
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 5 cut(s) 64, 281, 576, 607, 858
HspAI GCGC 1 cut(s) 49
Kzo9I GATC 4 cut(s) 88, 220, 318, 355
LguI GCTCTTC 1 cut(s) 120
LmnI GCTCC 3 cut(s) 115, 517, 833
Lsp1109I GCAGC 2 cut(s) 30, 439
LweI GCATC 7 cut(s) 60, 369, 488, 578, 640, 643, 805
MabI ACCWGGT 1 cut(s) 700
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 1 cut(s) 718
MalI GATC 4 cut(s) 90, 222, 320, 357
MbiI CCGCTC 1 cut(s) 110
MboI GATC 4 cut(s) 88, 220, 318, 355
MboII GAAGA 6 cut(s) 46, 137, 307, 365, 678, 705
MflI RGATCY 1 cut(s) 88
MluCI AATT 2 cut(s) 437, 552
MlyI GAGTC 1 cut(s) 511
MmeI TCCRAC 1 cut(s) 90
MnlI CCTC 8 cut(s) 83, 123, 208, 531, 600, 738, 802, 870
MroXI GAANNNNTTC 3 cut(s) 129, 428, 543
MseI TTAA 2 cut(s) 513, 870
MslI CAYNNNNRTG 2 cut(s) 282, 651
MspA1I CMGCKG 1 cut(s) 43
MspI CCGG 1 cut(s) 580
MspR9I CCNGG 3 cut(s) 580, 675, 702
Mva1269I GAATGC 2 cut(s) 399, 719
MvaI CCWGG 2 cut(s) 675, 702
MwoI GCNNNNNNNGC 4 cut(s) 57, 476, 485, 599
NciI CCSGG 1 cut(s) 580
NdeII GATC 4 cut(s) 88, 220, 318, 355
NlaIII CATG 5 cut(s) 64, 281, 576, 607, 858
NlaIV GGNNCC 3 cut(s) 191, 469, 519
NmeAIII GCCGAG 1 cut(s) 72
NspI RCATGY 2 cut(s) 64, 281
PciSI GCTCTTC 1 cut(s) 120
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PctI GAATGC 2 cut(s) 399, 719
PdmI GAANNNNTTC 3 cut(s) 129, 428, 543
PfeI GAWTC 5 cut(s) 343, 611, 683, 707, 803
PkrI GCNGC 4 cut(s) 45, 48, 109, 454
PleI GAGTC 1 cut(s) 510
PpsI GAGTC 1 cut(s) 510
PpuMI RGGWCCY 1 cut(s) 190
Psp5II RGGWCCY 1 cut(s) 190
Psp6I CCWGG 2 cut(s) 673, 700
PspGI CCWGG 2 cut(s) 673, 700
PspN4I GGNNCC 3 cut(s) 191, 469, 519
PspPI GGNCC 2 cut(s) 190, 468
PspPPI RGGWCCY 1 cut(s) 190
PsuI RGATCY 1 cut(s) 88
RsaI GTAC 2 cut(s) 151, 204
RsaNI GTAC 2 cut(s) 150, 203
RseI CAYNNNNRTG 2 cut(s) 282, 651
SapI GCTCTTC 1 cut(s) 120
SaqAI TTAA 2 cut(s) 513, 870
SatI GCNGC 4 cut(s) 44, 47, 108, 453
Sau3AI GATC 4 cut(s) 88, 220, 318, 355
Sau96I GGNCC 2 cut(s) 190, 468
ScaI AGTACT 1 cut(s) 151
SchI GAGTC 1 cut(s) 511
ScrFI CCNGG 3 cut(s) 580, 675, 702
SexAI ACCWGGT 1 cut(s) 700
SfaNI GCATC 7 cut(s) 60, 369, 488, 578, 640, 643, 805
SinI GGWCC 1 cut(s) 190
SmiMI CAYNNNNRTG 2 cut(s) 282, 651
SmlI CTYRAG 1 cut(s) 775
SmoI CTYRAG 1 cut(s) 775
Sse9I AATT 2 cut(s) 437, 552
SsiI CCGC 3 cut(s) 41, 46, 108
StyD4I CCNGG 3 cut(s) 578, 673, 700
StyI CCWWGG 2 cut(s) 238, 731
TaaI ACNGT 3 cut(s) 429, 821, 881
TaiI ACGT 1 cut(s) 264
TaqI TCGA 4 cut(s) 5, 393, 691, 792
TasI AATT 2 cut(s) 437, 552
TatI WGTACW 2 cut(s) 149, 202
TauI GCSGC 2 cut(s) 49, 110
TfiI GAWTC 5 cut(s) 343, 611, 683, 707, 803
Tru1I TTAA 2 cut(s) 513, 870
Tru9I TTAA 2 cut(s) 513, 870
TscAI CASTG 2 cut(s) 503, 826
TseI GCWGC 2 cut(s) 43, 452
TspDTI ATGAA 2 cut(s) 461, 828
TspGWI ACGGA 1 cut(s) 91
TspRI CASTG 2 cut(s) 503, 826
VpaK11BI GGWCC 1 cut(s) 190
XceI RCATGY 2 cut(s) 64, 281
XmnI GAANNNNTTC 3 cut(s) 129, 428, 543
ZrmI AGTACT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.