pycom10g09770

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002121
Physical Location & Seq
Reverse (-)
12977433 .. 12977881
449 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 408 bp
ATGGCACTAGGTCAAGTTCATCCGTCTCATGCTCAAAACTCACAGCAAGACTTCCTCAACGCACACAACGCGGCGCGAGCGCAAATCTACGTGCCGGATATCGTGTGGGACAACTCCGTGTCGGCGTATGCTCAAAACTCCGCCAACTCGAGAATTGGTGACTGCAATTTGGTGCATTCAAATTCCAACGGGACTTACGGCAAGAACTTTGCGAAAGGGAGTGGCTCGTTCACGGGCGTGGCGGCTGTGACCTTGTGGGTGGCGGAGAAGCCTAACTACGATTACAACTCTAATTCTTGTGTGGGCAGCAATTTGGGGTGTGCTAGGGTTCAGTGCACCAACGGGTGGTGGTTTGTGACTTGCAACTATTCCCCACCTGGAAACTACGTTGGGCAACGTCCTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

136

Amino Acids

14.61

Weight (kDa)

7.66

Isoelectric Point (pI)

22.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 19 - 123 3.2e-08 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 345
AccII CGCG 2 cut(s) 71, 76
AciI CCGC 4 cut(s) 71, 141, 242, 263
AcsI RAATTY 1 cut(s) 181
AfiI CCNNNNNNNGG 1 cut(s) 345
AgsI TTSAA 1 cut(s) 180
AjnI CCWGG 1 cut(s) 376
AleI CACNNNNGTG 1 cut(s) 236
Alw21I GWGCWC 1 cut(s) 338
Alw26I GTCTC 1 cut(s) 30
Alw44I GTGCAC 1 cut(s) 334
Ama87I CYCGRG 1 cut(s) 148
ApaLI GTGCAC 1 cut(s) 334
ApeKI GCWGC 1 cut(s) 306
ApoI RAATTY 1 cut(s) 181
AspLEI GCGC 2 cut(s) 76, 82
AsuHPI GGTGA 1 cut(s) 170
AvaI CYCGRG 1 cut(s) 148
BaeGI GKGCMC 1 cut(s) 338
BarI GAAGNNNNNNTAC 2 cut(s) 260, 292
Bbv12I GWGCWC 1 cut(s) 338
BbvI GCAGC 1 cut(s) 318
BceAI ACGGC 1 cut(s) 214
BciT130I CCWGG 1 cut(s) 378
BcoDI GTCTC 1 cut(s) 30
BfaI CTAG 2 cut(s) 8, 324
BisI GCNGC 3 cut(s) 72, 243, 307
BlsI GCNGC 3 cut(s) 73, 244, 308
Bme1390I CCNGG 1 cut(s) 378
BmeT110I CYCGRG 1 cut(s) 148
BmrFI CCNGG 1 cut(s) 378
BsaAI YACGTR 1 cut(s) 91
Bsc4I CCNNNNNNNGG 1 cut(s) 345
BseBI CCWGG 1 cut(s) 378
BseGI GGATG 1 cut(s) 19
BseLI CCNNNNNNNGG 1 cut(s) 345
BseSI GKGCMC 1 cut(s) 338
BseXI GCAGC 1 cut(s) 318
Bsh1236I CGCG 2 cut(s) 71, 76
BsiHKAI GWGCWC 1 cut(s) 338
BsiHKCI CYCGRG 1 cut(s) 148
BsiSI CCGG 1 cut(s) 95
BslFI GGGAC 2 cut(s) 122, 205
BslI CCNNNNNNNGG 1 cut(s) 345
BsmAI GTCTC 1 cut(s) 30
BsmBI CGTCTC 1 cut(s) 30
BsmFI GGGAC 2 cut(s) 122, 205
BsmI GAATGC 1 cut(s) 175
BsoBI CYCGRG 1 cut(s) 148
Bsp1286I GDGCHC 1 cut(s) 338
BspACI CCGC 4 cut(s) 71, 141, 242, 263
BspFNI CGCG 2 cut(s) 71, 76
Bst2UI CCWGG 1 cut(s) 378
BstBAI YACGTR 1 cut(s) 91
BstC8I GCNNGC 1 cut(s) 78
BstF5I GGATG 1 cut(s) 19
BstFNI CGCG 2 cut(s) 71, 76
BstHHI GCGC 2 cut(s) 76, 82
BstMAI GTCTC 1 cut(s) 30
BstMWI GCNNNNNNNGC 2 cut(s) 68, 77
BstNI CCWGG 1 cut(s) 378
BstSCI CCNGG 1 cut(s) 376
BstSLI GKGCMC 1 cut(s) 338
BstUI CGCG 2 cut(s) 71, 76
BstV1I GCAGC 1 cut(s) 318
BtsCI GGATG 1 cut(s) 19
BtsIMutI CAGTG 1 cut(s) 338
Cac8I GCNNGC 1 cut(s) 78
CfoI GCGC 2 cut(s) 76, 82
CviAII CATG 1 cut(s) 29
CviJI RGCY 3 cut(s) 225, 245, 271
CviKI_1 RGCY 3 cut(s) 225, 245, 271
EciI GGCGGA 2 cut(s) 130, 278
Eco32I GATATC 1 cut(s) 100
Eco88I CYCGRG 1 cut(s) 148
EcoRII CCWGG 1 cut(s) 376
EcoRV GATATC 1 cut(s) 100
Esp3I CGTCTC 1 cut(s) 30
FaeI CATG 1 cut(s) 32
FaiI YATR 2 cut(s) 30, 129
FaqI GGGAC 2 cut(s) 122, 205
FatI CATG 1 cut(s) 28
Fnu4HI GCNGC 3 cut(s) 72, 243, 307
FokI GGATG 1 cut(s) 6
Fsp4HI GCNGC 3 cut(s) 72, 243, 307
FspBI CTAG 2 cut(s) 8, 324
GlaI GCGC 2 cut(s) 75, 81
GluI GCNGC 3 cut(s) 72, 243, 307
HapII CCGG 1 cut(s) 95
HhaI GCGC 2 cut(s) 76, 82
Hin1II CATG 1 cut(s) 32
Hin6I GCGC 2 cut(s) 74, 80
HinP1I GCGC 2 cut(s) 74, 80
HpaII CCGG 1 cut(s) 95
HphI GGTGA 1 cut(s) 170
Hpy166II GTNNAC 2 cut(s) 231, 336
Hpy188III TCNNGA 1 cut(s) 150
Hpy8I GTNNAC 2 cut(s) 231, 336
HpyCH4IV ACGT 3 cut(s) 90, 387, 397
HpyCH4V TGCA 4 cut(s) 165, 175, 336, 363
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 77
HpySE526I ACGT 3 cut(s) 90, 387, 397
Hsp92II CATG 1 cut(s) 32
HspAI GCGC 2 cut(s) 74, 80
LpnPI CCDG 3 cut(s) 108, 363, 390
Lsp1109I GCAGC 1 cut(s) 318
MaeI CTAG 2 cut(s) 8, 324
MaeII ACGT 3 cut(s) 90, 387, 397
MaeIII GTNAC 3 cut(s) 158, 247, 355
MhlI GDGCHC 1 cut(s) 338
MluCI AATT 5 cut(s) 153, 166, 181, 292, 310
MmeI TCCRAC 1 cut(s) 210
MnlI CCTC 1 cut(s) 65
MslI CAYNNNNRTG 1 cut(s) 236
MspI CCGG 1 cut(s) 95
MspR9I CCNGG 1 cut(s) 378
Mva1269I GAATGC 1 cut(s) 175
MvaI CCWGG 1 cut(s) 378
MvnI CGCG 2 cut(s) 71, 76
MwoI GCNNNNNNNGC 2 cut(s) 68, 77
NlaIII CATG 1 cut(s) 32
NmuCI GTSAC 3 cut(s) 158, 247, 355
OliI CACNNNNGTG 1 cut(s) 236
PaeR7I CTCGAG 1 cut(s) 148
PctI GAATGC 1 cut(s) 175
PflMI CCANNNNNTGG 1 cut(s) 345
PkrI GCNGC 3 cut(s) 73, 244, 308
Ppu21I YACGTR 1 cut(s) 91
Psp6I CCWGG 1 cut(s) 376
PspGI CCWGG 1 cut(s) 376
RseI CAYNNNNRTG 1 cut(s) 236
SatI GCNGC 3 cut(s) 72, 243, 307
ScrFI CCNGG 1 cut(s) 378
SduI GDGCHC 1 cut(s) 338
SetI ASST 6 cut(s) 13, 93, 254, 379, 390, 400
Sfr274I CTCGAG 1 cut(s) 148
SlaI CTCGAG 1 cut(s) 148
SmiMI CAYNNNNRTG 1 cut(s) 236
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
Sse9I AATT 5 cut(s) 153, 166, 181, 292, 310
SsiI CCGC 4 cut(s) 71, 141, 242, 263
SspMI CTAG 2 cut(s) 8, 324
StyD4I CCNGG 1 cut(s) 376
TaiI ACGT 3 cut(s) 93, 390, 400
TaqI TCGA 1 cut(s) 149
TasI AATT 5 cut(s) 153, 166, 181, 292, 310
TauI GCSGC 2 cut(s) 74, 245
TscAI CASTG 1 cut(s) 338
TseFI GTSAC 3 cut(s) 158, 247, 355
TseI GCWGC 1 cut(s) 306
Tsp45I GTSAC 3 cut(s) 158, 247, 355
TspDTI ATGAA 1 cut(s) 8
TspGWI ACGGA 2 cut(s) 12, 106
TspRI CASTG 1 cut(s) 338
Van91I CCANNNNNTGG 1 cut(s) 345
VneI GTGCAC 1 cut(s) 334
XapI RAATTY 1 cut(s) 181
XhoI CTCGAG 1 cut(s) 148
XspI CTAG 2 cut(s) 8, 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.