pycom10g12420

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002062
Physical Location & Seq
Forward (+)
15924911 .. 15926325
1415 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1371 bp
ATGACCTCACCTTCAACATCAAGTTTCCCATCAGAAAACAATGGAGGAAGAGATGATCCGGATGTGGTCATTCCAATTGAATTGCCAGACGATTTTAATATAACTCAGCTGATGTCGTCAATGGAGGAACGTCTTAAGTCAGTTGAGAAAACAGATTGGCTACTCCGCCCATCAGCTGTCAACAGGAAGCACTACCACCCGCATATAGTCTCCATCGGCCCATATCACCGCGGTCGCCAACATCTGGAGATGATGCAGCAACACAAATGGAGATATCTTTGCGACTTACTTGATCGAACACCATCAACTGGCCCCAAACTCGCTGATTACTTGCAGGTTGTAGCTTTGGGGGAGGAAAAGATACGAGGGTGCTATTCTGAGACCCTCAACTACTTGAGCAAAAGCGAGCTCGTTGAGATGATGGTACTAGATGGTCTCTTCATTATCGAAGTCTTCTCCAGATTTGAAGATTTTGCAGAAAATTTGGATGACACCATCTTTAGCTCGGAATGGATATTGCCTCAACTCAGGCTAGACTTTCTTCGGCTAGAAAACCAAATTCCTTTTTTCGTTCTTCAACAGTTATTTCATATGTCGAAACCTTCAAGAGAGGATACTTATCAGTGTTCGTCCCTTAGCAAGGCTGCTTTAAAATTCCTTAACTACGTAGTGGAACAAAGATCGGACGAAGTCTTGGAGCAATTTTTCAATGTAGGCGGAGTACATCTACTCGATTTATTTCACGTGACTTTTACCGCCGTTATTCCTGATCTTCCTCGAGAAAATCCTCCTCAGTCAGTTGAATTGATCGGAACAGTGAAAAAGCTTCATCAAGCGGGAATTAAGTTCAAGAACAGCAAGGCAACCAGCTTCCTAGATATCAAATTCTGCAATGGAGTGCTAGAAATTCCACACGTAATAGTAGACGATTACAGTATCCATGTACTCCTGAATTTCGTTGCATTCGAACAATGTTATAAGGATTGCGACAAGCACATAACCACTTATGCTGCATTCATGAACTGCCTCATCCGCAGGCCTGCGGATGCAACCTTCCTCTCTGAGAAGAACATCATCGAGAATTATCTTGGATTCGACGAGGAGCTCACTCAATTCTTCAACAATATGAGCAAAGATGTTGGTTTCTGCGTCATTGGGAGTTACTTACAGCAGGTATTCAAGGATATGAACGAGCACTGCAATAATGTTTGGAACGTGCAATGGGCAGGTTTTATGTTGAAGTATTTTAAAACCCCTTGGTCTTTTATGTCTGCCTCAGCTGTTTTGATTGCCTTATTACTCACACTAATCCAGACCGTTTTATCCATTTTTGAAATTAACGGTCCACAGGAGCGTAGCGGAGGGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

457

Amino Acids

52.53

Weight (kDa)

5.34

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 59 - 434 1.8e-110 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 978
Acc36I ACCTGC 3 cut(s) 325, 1162, 1217
AccB7I CCANNNNNTGG 2 cut(s) 244, 308
AccI GTMKAC 1 cut(s) 924
AccII CGCG 1 cut(s) 231
AccIII TCCGGA 1 cut(s) 58
AclWI GGATC 1 cut(s) 50
AcsI RAATTY 6 cut(s) 481, 558, 653, 884, 906, 952
AcvI CACGTG 1 cut(s) 745
AfaI GTAC 3 cut(s) 426, 723, 945
AfiI CCNNNNNNNGG 3 cut(s) 244, 308, 640
AflII CTTAAG 1 cut(s) 134
AflIII ACRYGT 1 cut(s) 913
AluBI AGCT 9 cut(s) 109, 176, 344, 409, 504, 826, 870, 1105, 1280
AluI AGCT 9 cut(s) 109, 176, 344, 409, 504, 826, 870, 1105, 1280
Alw21I GWGCWC 3 cut(s) 411, 1107, 1197
Alw26I GTCTC 3 cut(s) 214, 374, 440
AlwI GGATC 1 cut(s) 50
Ama87I CYCGRG 1 cut(s) 777
Aor13HI TCCGGA 1 cut(s) 58
AoxI GGCC 3 cut(s) 217, 310, 1037
ApeKI GCWGC 3 cut(s) 256, 644, 1010
ApoI RAATTY 6 cut(s) 481, 558, 653, 884, 906, 952
AspS9I GGNCC 3 cut(s) 218, 311, 1343
AsuHPI GGTGA 1 cut(s) 218
AsuII TTCGAA 1 cut(s) 966
AvaI CYCGRG 1 cut(s) 777
AvaII GGWCC 1 cut(s) 1343
BaeGI GKGCMC 1 cut(s) 1368
BaeI ACNNNNGTAYC 2 cut(s) 353, 386
BanII GRGCYC 2 cut(s) 411, 1107
BbrPI CACGTG 1 cut(s) 745
BbsI GAAGAC 1 cut(s) 445
Bbv12I GWGCWC 3 cut(s) 411, 1107, 1197
BbvCI CCTCAGC 1 cut(s) 1276
BbvI GCAGC 3 cut(s) 268, 631, 997
BccI CCATC 7 cut(s) 37, 178, 221, 310, 415, 425, 503
BceAI ACGGC 1 cut(s) 743
BciVI GTATCC 2 cut(s) 607, 947
BcoDI GTCTC 3 cut(s) 214, 374, 440
BfaI CTAG 6 cut(s) 428, 533, 548, 875, 902, 1369
BfrI CTTAAG 1 cut(s) 134
BfuAI ACCTGC 3 cut(s) 325, 1162, 1217
BfuI GTATCC 2 cut(s) 607, 947
BisI GCNGC 3 cut(s) 257, 645, 1011
BlsI GCNGC 3 cut(s) 258, 646, 1012
Bme18I GGWCC 1 cut(s) 1343
BmeT110I CYCGRG 1 cut(s) 777
BmgT120I GGNCC 3 cut(s) 218, 311, 1343
BmiI GGNNCC 1 cut(s) 313
BmsI GCATC 2 cut(s) 243, 1036
BpiI GAAGAC 1 cut(s) 445
BpmI CTGGAG 2 cut(s) 266, 442
Bpu10I CCTNAGC 2 cut(s) 635, 1276
Bpu14I TTCGAA 1 cut(s) 966
BpuEI CTTGAG 1 cut(s) 415
BsaAI YACGTR 3 cut(s) 667, 745, 916
BsaBI GATNNNNATC 1 cut(s) 618
BsaI GGTCTC 2 cut(s) 374, 440
BsaJI CCNNGG 2 cut(s) 229, 1256
BsaWI WCCGGW 1 cut(s) 58
Bsc4I CCNNNNNNNGG 3 cut(s) 244, 308, 640
Bse1I ACTGG 1 cut(s) 313
Bse3DI GCAATG 2 cut(s) 898, 1226
Bse8I GATNNNNATC 1 cut(s) 618
BseAI TCCGGA 1 cut(s) 58
BseDI CCNNGG 2 cut(s) 229, 1256
BseGI GGATG 4 cut(s) 67, 493, 1029, 1051
BseJI GATNNNNATC 1 cut(s) 618
BseLI CCNNNNNNNGG 3 cut(s) 244, 308, 640
BseMI GCAATG 2 cut(s) 898, 1226
BseMII CTCAG 6 cut(s) 119, 369, 541, 806, 1053, 1290
BseNI ACTGG 1 cut(s) 313
BseRI GAGGAG 2 cut(s) 780, 1115
BseSI GKGCMC 1 cut(s) 1368
BseXI GCAGC 3 cut(s) 268, 631, 997
Bsh1236I CGCG 1 cut(s) 231
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 3 cut(s) 219, 312, 1039
BsiEI CGRYCG 1 cut(s) 235
BsiHKAI GWGCWC 3 cut(s) 411, 1107, 1197
BsiHKCI CYCGRG 1 cut(s) 777
BsiSI CCGG 1 cut(s) 59
BslFI GGGAC 1 cut(s) 616
BslI CCNNNNNNNGG 3 cut(s) 244, 308, 640
BsmAI GTCTC 3 cut(s) 214, 374, 440
BsmFI GGGAC 1 cut(s) 616
BsmI GAATGC 2 cut(s) 962, 1013
BsnI GGCC 3 cut(s) 219, 312, 1039
Bso31I GGTCTC 2 cut(s) 374, 440
BsoBI CYCGRG 1 cut(s) 777
Bsp119I TTCGAA 1 cut(s) 966
Bsp1286I GDGCHC 4 cut(s) 411, 1107, 1197, 1368
Bsp13I TCCGGA 1 cut(s) 58
Bsp143I GATC 5 cut(s) 55, 292, 680, 769, 807
BspANI GGCC 3 cut(s) 219, 312, 1039
BspCNI CTCAG 6 cut(s) 118, 370, 540, 805, 1054, 1289
BspEI TCCGGA 1 cut(s) 58
BspFNI CGCG 1 cut(s) 231
BspHI TCATGA 1 cut(s) 1017
BspLI GGNNCC 1 cut(s) 313
BspMI ACCTGC 3 cut(s) 325, 1162, 1217
BspPI GGATC 1 cut(s) 50
BspT104I TTCGAA 1 cut(s) 966
BspTI CTTAAG 1 cut(s) 134
BspTNI GGTCTC 2 cut(s) 374, 440
BsrDI GCAATG 2 cut(s) 898, 1226
BsrI ACTGG 1 cut(s) 313
BssECI CCNNGG 2 cut(s) 229, 1256
BssMI GATC 5 cut(s) 55, 292, 680, 769, 807
BssT1I CCWWGG 1 cut(s) 1256
Bst4CI ACNGT 5 cut(s) 582, 817, 935, 1318, 1343
Bst6I CTCTTC 2 cut(s) 43, 443
BstAFI CTTAAG 1 cut(s) 134
BstBAI YACGTR 3 cut(s) 667, 745, 916
BstBI TTCGAA 1 cut(s) 966
BstC8I GCNNGC 3 cut(s) 407, 1037, 1041
BstDEI CTNAG 7 cut(s) 105, 378, 527, 635, 792, 1062, 1276
BstDSI CCRYGG 1 cut(s) 229
BstENI CCTNNNNNAGG 1 cut(s) 638
BstF5I GGATG 4 cut(s) 67, 493, 1029, 1051
BstFNI CGCG 1 cut(s) 231
BstKTI GATC 5 cut(s) 58, 295, 683, 772, 810
BstMAI GTCTC 3 cut(s) 214, 374, 440
BstMBI GATC 5 cut(s) 55, 292, 680, 769, 807
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 1 cut(s) 1032
BstSLI GKGCMC 1 cut(s) 1368
BstSNI TACGTA 1 cut(s) 667
BstUI CGCG 1 cut(s) 231
BstV1I GCAGC 3 cut(s) 268, 631, 997
BstV2I GAAGAC 1 cut(s) 445
BsuI GTATCC 2 cut(s) 607, 947
BsuRI GGCC 3 cut(s) 219, 312, 1039
BtgI CCRYGG 1 cut(s) 229
BtsCI GGATG 4 cut(s) 67, 493, 1029, 1051
BtsI GCAGTG 1 cut(s) 1195
BtsIMutI CAGTG 3 cut(s) 629, 822, 1195
BveI ACCTGC 3 cut(s) 325, 1162, 1217
Cac8I GCNNGC 3 cut(s) 407, 1037, 1041
CciI TCATGA 1 cut(s) 1017
Cfr13I GGNCC 3 cut(s) 218, 311, 1343
Cfr42I CCGCGG 1 cut(s) 232
CseI GACGC 1 cut(s) 1138
Csp6I GTAC 3 cut(s) 425, 722, 944
CviAII CATG 2 cut(s) 941, 1018
CviQI GTAC 3 cut(s) 425, 722, 944
DdeI CTNAG 7 cut(s) 105, 378, 527, 635, 792, 1062, 1276
DpnI GATC 5 cut(s) 57, 294, 682, 771, 809
DpnII GATC 5 cut(s) 55, 292, 680, 769, 807
DraI TTTAAA 2 cut(s) 651, 1249
Eam1104I CTCTTC 2 cut(s) 43, 443
EarI CTCTTC 2 cut(s) 43, 443
EciI GGCGGA 2 cut(s) 155, 732
Ecl136II GAGCTC 2 cut(s) 409, 1105
Eco105I TACGTA 1 cut(s) 667
Eco130I CCWWGG 1 cut(s) 1256
Eco147I AGGCCT 1 cut(s) 1039
Eco24I GRGCYC 2 cut(s) 411, 1107
Eco31I GGTCTC 2 cut(s) 374, 440
Eco32I GATATC 2 cut(s) 275, 880
Eco47I GGWCC 1 cut(s) 1343
Eco53kI GAGCTC 2 cut(s) 409, 1105
Eco72I CACGTG 1 cut(s) 745
Eco88I CYCGRG 1 cut(s) 777
EcoICRI GAGCTC 2 cut(s) 409, 1105
EcoNI CCTNNNNNAGG 1 cut(s) 638
EcoRV GATATC 2 cut(s) 275, 880
EcoT14I CCWWGG 1 cut(s) 1256
EcoT38I GRGCYC 2 cut(s) 411, 1107
ErhI CCWWGG 1 cut(s) 1256
FaeI CATG 2 cut(s) 944, 1021
FaqI GGGAC 1 cut(s) 616
FatI CATG 2 cut(s) 940, 1017
FauI CCCGC 2 cut(s) 207, 829
FauNDI CATATG 1 cut(s) 591
FblI GTMKAC 1 cut(s) 924
Fnu4HI GCNGC 3 cut(s) 257, 645, 1011
FokI GGATG 4 cut(s) 74, 500, 1016, 1058
FriOI GRGCYC 2 cut(s) 411, 1107
Fsp4HI GCNGC 3 cut(s) 257, 645, 1011
FspBI CTAG 6 cut(s) 428, 533, 548, 875, 902, 1369
GluI GCNGC 3 cut(s) 257, 645, 1011
GsuI CTGGAG 2 cut(s) 266, 442
HaeIII GGCC 3 cut(s) 219, 312, 1039
HapII CCGG 1 cut(s) 59
HgaI GACGC 1 cut(s) 1138
Hin1II CATG 2 cut(s) 944, 1021
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 824
HinfI GANTC 1 cut(s) 1092
HpaII CCGG 1 cut(s) 59
HphI GGTGA 1 cut(s) 218
Hpy166II GTNNAC 3 cut(s) 181, 925, 1346
Hpy188I TCNGA 6 cut(s) 34, 379, 508, 685, 812, 1063
Hpy8I GTNNAC 3 cut(s) 181, 925, 1346
Hpy99I CGWCG 1 cut(s) 1100
HpyAV CCTTC 3 cut(s) 21, 612, 1063
HpyCH4III ACNGT 5 cut(s) 582, 817, 935, 1318, 1343
HpyCH4IV ACGT 5 cut(s) 130, 666, 744, 915, 1215
HpyCH4V TGCA 9 cut(s) 256, 334, 476, 891, 962, 1013, 1049, 1200, 1219
HpyF10VI GCNNNNNNNGC 1 cut(s) 1032
HpyF3I CTNAG 7 cut(s) 105, 378, 527, 635, 792, 1062, 1276
HpySE526I ACGT 5 cut(s) 130, 666, 744, 915, 1215
Hsp92II CATG 2 cut(s) 944, 1021
Kpn2I TCCGGA 1 cut(s) 58
KspI CCGCGG 1 cut(s) 232
Kzo9I GATC 5 cut(s) 55, 292, 680, 769, 807
LmnI GCTCC 3 cut(s) 697, 1102, 1351
Lsp1109I GCAGC 3 cut(s) 268, 631, 997
LweI GCATC 2 cut(s) 243, 1036
MaeI CTAG 6 cut(s) 428, 533, 548, 875, 902, 1369
MaeII ACGT 5 cut(s) 130, 666, 744, 915, 1215
MaeIII GTNAC 2 cut(s) 745, 1160
MalI GATC 5 cut(s) 57, 294, 682, 771, 809
MboI GATC 5 cut(s) 55, 292, 680, 769, 807
MboII GAAGA 9 cut(s) 60, 430, 445, 479, 533, 566, 764, 1078, 1108
MfeI CAATTG 1 cut(s) 75
MhlI GDGCHC 4 cut(s) 411, 1107, 1197, 1368
MroI TCCGGA 1 cut(s) 58
MseI TTAA 7 cut(s) 96, 135, 650, 660, 843, 1248, 1338
MspA1I CMGCKG 4 cut(s) 109, 176, 231, 1280
MspCI CTTAAG 1 cut(s) 134
MspI CCGG 1 cut(s) 59
MunI CAATTG 1 cut(s) 75
Mva1269I GAATGC 2 cut(s) 962, 1013
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 1 cut(s) 1032
NdeI CATATG 1 cut(s) 591
NdeII GATC 5 cut(s) 55, 292, 680, 769, 807
NlaIII CATG 2 cut(s) 944, 1021
NlaIV GGNNCC 1 cut(s) 313
NmuCI GTSAC 1 cut(s) 745
NspV TTCGAA 1 cut(s) 966
PaeR7I CTCGAG 1 cut(s) 777
PagI TCATGA 1 cut(s) 1017
PceI AGGCCT 1 cut(s) 1039
PcsI WCGNNNNNNNCGW 1 cut(s) 963
PctI GAATGC 2 cut(s) 962, 1013
PfeI GAWTC 1 cut(s) 1092
PflFI GACNNNGTC 1 cut(s) 689
PflMI CCANNNNNTGG 2 cut(s) 244, 308
PkrI GCNGC 3 cut(s) 258, 646, 1012
PmaCI CACGTG 1 cut(s) 745
PmlI CACGTG 1 cut(s) 745
Ppu21I YACGTR 3 cut(s) 667, 745, 916
PsiI TTATAA 1 cut(s) 978
Psp124BI GAGCTC 2 cut(s) 411, 1107
PspCI CACGTG 1 cut(s) 745
PspN4I GGNNCC 1 cut(s) 313
PspPI GGNCC 3 cut(s) 218, 311, 1343
PsyI GACNNNGTC 1 cut(s) 689
PvuII CAGCTG 3 cut(s) 109, 176, 1280
RsaI GTAC 3 cut(s) 426, 723, 945
RsaNI GTAC 3 cut(s) 425, 722, 944
SacI GAGCTC 2 cut(s) 411, 1107
SacII CCGCGG 1 cut(s) 232
SaqAI TTAA 7 cut(s) 96, 135, 650, 660, 843, 1248, 1338
SatI GCNGC 3 cut(s) 257, 645, 1011
Sau3AI GATC 5 cut(s) 55, 292, 680, 769, 807
Sau96I GGNCC 3 cut(s) 218, 311, 1343
SduI GDGCHC 4 cut(s) 411, 1107, 1197, 1368
SfaNI GCATC 2 cut(s) 243, 1036
Sfr274I CTCGAG 1 cut(s) 777
Sfr303I CCGCGG 1 cut(s) 232
SfuI TTCGAA 1 cut(s) 966
SgrBI CCGCGG 1 cut(s) 232
SinI GGWCC 1 cut(s) 1343
SlaI CTCGAG 1 cut(s) 777
SmlI CTYRAG 3 cut(s) 134, 394, 777
SmoI CTYRAG 3 cut(s) 134, 394, 777
SnaBI TACGTA 1 cut(s) 667
SseBI AGGCCT 1 cut(s) 1039
SspMI CTAG 6 cut(s) 428, 533, 548, 875, 902, 1369
SstI GAGCTC 2 cut(s) 411, 1107
StuI AGGCCT 1 cut(s) 1039
StyI CCWWGG 1 cut(s) 1256
TaaI ACNGT 5 cut(s) 582, 817, 935, 1318, 1343
TaiI ACGT 5 cut(s) 133, 669, 747, 918, 1218
TaqI TCGA 8 cut(s) 295, 447, 596, 732, 778, 966, 1077, 1095
TatI WGTACW 2 cut(s) 721, 943
TfiI GAWTC 1 cut(s) 1092
Tru1I TTAA 7 cut(s) 96, 135, 650, 660, 843, 1248, 1338
Tru9I TTAA 7 cut(s) 96, 135, 650, 660, 843, 1248, 1338
TscAI CASTG 3 cut(s) 629, 822, 1202
TseFI GTSAC 1 cut(s) 745
TseI GCWGC 3 cut(s) 256, 644, 1010
Tsp45I GTSAC 1 cut(s) 745
TspDTI ATGAA 6 cut(s) 430, 578, 818, 1006, 1034, 1202
TspRI CASTG 3 cut(s) 629, 822, 1202
Tth111I GACNNNGTC 1 cut(s) 689
Van91I CCANNNNNTGG 2 cut(s) 244, 308
Vha464I CTTAAG 1 cut(s) 134
VpaK11BI GGWCC 1 cut(s) 1343
XagI CCTNNNNNAGG 1 cut(s) 638
XapI RAATTY 6 cut(s) 481, 558, 653, 884, 906, 952
XhoI CTCGAG 1 cut(s) 777
XmiI GTMKAC 1 cut(s) 924
XspI CTAG 6 cut(s) 428, 533, 548, 875, 902, 1369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.