pycom10g14050

4F5 protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002526
Physical Location & Seq
Forward (+)
17463684 .. 17464703
1020 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 252 bp
ATGTGCGAAAAAAAAGCGCGGCTTCTCGAGACTTCCATAGATTCGTTGGTTCCCAGCATCTCCTCCTTTTTGGTCACCACCTCCCCTCCGGCCCCAAAACCCAGTACCATCAACTTCTTCTTCTTCAGCCTCTCCGTGTTTTCTCTCCGAGCTCAGAAATCGATCAGGGACGCAAAAGCGCTGCAGGAGAAGGCGAAGAAGAAGGCAGCGGCCGAGCAGGCCGGAGGGATCAACGCCGGTGGGAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4F5

Protein Analysis

84

Amino Acids

8.89

Weight (kDa)

10.09

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 19
AciI CCGC 2 cut(s) 19, 209
AclWI GGATC 1 cut(s) 236
AcoI YGGCCR 1 cut(s) 210
AcuI CTGAAG 1 cut(s) 109
AfaI GTAC 1 cut(s) 106
AfeI AGCGCT 1 cut(s) 180
AluBI AGCT 1 cut(s) 152
AluI AGCT 1 cut(s) 152
Alw21I GWGCWC 1 cut(s) 154
Alw26I GTCTC 1 cut(s) 23
AlwI GGATC 1 cut(s) 236
Ama87I CYCGRG 1 cut(s) 26
Aor51HI AGCGCT 1 cut(s) 180
AoxI GGCC 3 cut(s) 90, 210, 219
ApeKI GCWGC 2 cut(s) 181, 206
AspLEI GCGC 2 cut(s) 19, 181
AspS9I GGNCC 1 cut(s) 91
AsuHPI GGTGA 1 cut(s) 67
AvaI CYCGRG 1 cut(s) 26
BanII GRGCYC 1 cut(s) 154
Bbv12I GWGCWC 1 cut(s) 154
BbvI GCAGC 2 cut(s) 168, 218
BccI CCATC 1 cut(s) 116
BcoDI GTCTC 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 182
BfoI RGCGCY 1 cut(s) 182
BglI GCCNNNNNGGC 1 cut(s) 218
BisI GCNGC 4 cut(s) 20, 182, 207, 210
BlsI GCNGC 4 cut(s) 21, 183, 208, 211
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 2 cut(s) 51, 93
BmrI ACTGGG 1 cut(s) 96
BmsI GCATC 1 cut(s) 66
BmuI ACTGGG 1 cut(s) 96
Bsa29I ATCGAT 1 cut(s) 161
BsaXI ACNNNNNCTCC 2 cut(s) 70, 100
Bse118I RCCGGY 1 cut(s) 236
Bse1I ACTGG 1 cut(s) 102
BseCI ATCGAT 1 cut(s) 161
BseMII CTCAG 1 cut(s) 167
BseNI ACTGG 1 cut(s) 102
BseRI GAGGAG 1 cut(s) 52
BseX3I CGGCCG 1 cut(s) 210
BseXI GCAGC 2 cut(s) 168, 218
BseYI CCCAGC 1 cut(s) 53
Bsh1236I CGCG 1 cut(s) 19
Bsh1285I CGRYCG 1 cut(s) 213
BshFI GGCC 3 cut(s) 92, 212, 221
BshVI ATCGAT 1 cut(s) 161
BsiEI CGRYCG 1 cut(s) 213
BsiHKAI GWGCWC 1 cut(s) 154
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 3 cut(s) 89, 222, 237
BslFI GGGAC 1 cut(s) 182
BsmAI GTCTC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 182
BsnI GGCC 3 cut(s) 92, 212, 221
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 154
Bsp143I GATC 2 cut(s) 162, 228
BspACI CCGC 2 cut(s) 19, 209
BspANI GGCC 3 cut(s) 92, 212, 221
BspCNI CTCAG 1 cut(s) 166
BspDI ATCGAT 1 cut(s) 161
BspFNI CGCG 1 cut(s) 19
BspLI GGNNCC 2 cut(s) 51, 93
BspMAI CTGCAG 1 cut(s) 186
BspPI GGATC 1 cut(s) 236
BsrFI RCCGGY 1 cut(s) 236
BsrI ACTGG 1 cut(s) 102
BssAI RCCGGY 1 cut(s) 236
BssMI GATC 2 cut(s) 162, 228
BstC8I GCNNGC 1 cut(s) 219
BstDEI CTNAG 1 cut(s) 153
BstEII GGTNACC 1 cut(s) 73
BstFNI CGCG 1 cut(s) 19
BstH2I RGCGCY 1 cut(s) 182
BstHHI GCGC 2 cut(s) 19, 181
BstKTI GATC 2 cut(s) 165, 231
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 2 cut(s) 162, 228
BstMCI CGRYCG 1 cut(s) 213
BstMWI GCNNNNNNNGC 1 cut(s) 218
BstPI GGTNACC 1 cut(s) 73
BstSFI CTRYAG 1 cut(s) 182
BstUI CGCG 1 cut(s) 19
BstV1I GCAGC 2 cut(s) 168, 218
BstZI CGGCCG 1 cut(s) 210
Bsu15I ATCGAT 1 cut(s) 161
BsuRI GGCC 3 cut(s) 92, 212, 221
BsuTUI ATCGAT 1 cut(s) 161
Cac8I GCNNGC 1 cut(s) 219
CfoI GCGC 2 cut(s) 19, 181
Cfr10I RCCGGY 1 cut(s) 236
Cfr13I GGNCC 1 cut(s) 91
ClaI ATCGAT 1 cut(s) 161
CseI GACGC 1 cut(s) 179
Csp6I GTAC 1 cut(s) 105
CspCI CAANNNNNGTGG 1 cut(s) 220
CviJI RGCY 6 cut(s) 22, 92, 129, 152, 212, 221
CviKI_1 RGCY 6 cut(s) 22, 92, 129, 152, 212, 221
CviQI GTAC 1 cut(s) 105
DdeI CTNAG 1 cut(s) 153
DpnI GATC 2 cut(s) 164, 230
DpnII GATC 2 cut(s) 162, 228
EaeI YGGCCR 1 cut(s) 210
EagI CGGCCG 1 cut(s) 210
Ecl136II GAGCTC 1 cut(s) 152
EclXI CGGCCG 1 cut(s) 210
Eco24I GRGCYC 1 cut(s) 154
Eco47III AGCGCT 1 cut(s) 180
Eco52I CGGCCG 1 cut(s) 210
Eco53kI GAGCTC 1 cut(s) 152
Eco57I CTGAAG 1 cut(s) 109
Eco88I CYCGRG 1 cut(s) 26
Eco91I GGTNACC 1 cut(s) 73
EcoICRI GAGCTC 1 cut(s) 152
EcoO65I GGTNACC 1 cut(s) 73
EcoT38I GRGCYC 1 cut(s) 154
FaiI YATR 1 cut(s) 38
FalI AAGNNNNNCTT 1 cut(s) 38
FaqI GGGAC 1 cut(s) 182
Fnu4HI GCNGC 4 cut(s) 20, 182, 207, 210
FriOI GRGCYC 1 cut(s) 154
Fsp4HI GCNGC 4 cut(s) 20, 182, 207, 210
GlaI GCGC 2 cut(s) 18, 180
GluI GCNGC 4 cut(s) 20, 182, 207, 210
GsaI CCCAGC 1 cut(s) 57
HaeII RGCGCY 1 cut(s) 182
HaeIII GGCC 3 cut(s) 92, 212, 221
HapII CCGG 3 cut(s) 89, 222, 237
HgaI GACGC 1 cut(s) 179
HhaI GCGC 2 cut(s) 19, 181
Hin6I GCGC 2 cut(s) 17, 179
HinP1I GCGC 2 cut(s) 17, 179
HinfI GANTC 1 cut(s) 41
HpaII CCGG 3 cut(s) 89, 222, 237
HphI GGTGA 1 cut(s) 67
Hpy188I TCNGA 2 cut(s) 149, 156
Hpy188III TCNNGA 2 cut(s) 26, 28
HpyAV CCTTC 2 cut(s) 184, 196
HpyCH4V TGCA 1 cut(s) 184
HpyF10VI GCNNNNNNNGC 1 cut(s) 218
HpyF3I CTNAG 1 cut(s) 153
HspAI GCGC 2 cut(s) 17, 179
Kzo9I GATC 2 cut(s) 162, 228
LpnPI CCDG 7 cut(s) 67, 102, 115, 151, 170, 203, 235
Lsp1109I GCAGC 2 cut(s) 168, 218
LweI GCATC 1 cut(s) 66
MaeIII GTNAC 1 cut(s) 73
MalI GATC 2 cut(s) 164, 230
MboI GATC 2 cut(s) 162, 228
MboII GAAGA 5 cut(s) 109, 112, 115, 208, 211
MhlI GDGCHC 1 cut(s) 154
MnlI CCTC 5 cut(s) 73, 91, 96, 140, 218
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 3 cut(s) 89, 222, 237
MvnI CGCG 1 cut(s) 19
MwoI GCNNNNNNNGC 1 cut(s) 218
NdeII GATC 2 cut(s) 162, 228
NlaIV GGNNCC 2 cut(s) 51, 93
NmeAIII GCCGAG 1 cut(s) 238
NmuCI GTSAC 1 cut(s) 73
PaeR7I CTCGAG 1 cut(s) 26
PfeI GAWTC 1 cut(s) 41
PkrI GCNGC 4 cut(s) 21, 183, 208, 211
Psp124BI GAGCTC 1 cut(s) 154
PspEI GGTNACC 1 cut(s) 73
PspFI CCCAGC 1 cut(s) 53
PspN4I GGNNCC 2 cut(s) 51, 93
PspPI GGNCC 1 cut(s) 91
PstI CTGCAG 1 cut(s) 186
RsaI GTAC 1 cut(s) 106
RsaNI GTAC 1 cut(s) 105
SacI GAGCTC 1 cut(s) 154
SatI GCNGC 4 cut(s) 20, 182, 207, 210
Sau3AI GATC 2 cut(s) 162, 228
Sau96I GGNCC 1 cut(s) 91
SduI GDGCHC 1 cut(s) 154
SetI ASST 2 cut(s) 83, 154
SfaNI GCATC 1 cut(s) 66
SfcI CTRYAG 1 cut(s) 182
SfiI GGCCNNNNNGGCC 1 cut(s) 218
Sfr274I CTCGAG 1 cut(s) 26
SgrAI CRCCGGYG 1 cut(s) 236
SlaI CTCGAG 1 cut(s) 26
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
SsiI CCGC 2 cut(s) 19, 209
SstI GAGCTC 1 cut(s) 154
TaqI TCGA 2 cut(s) 27, 161
TauI GCSGC 2 cut(s) 22, 212
TfiI GAWTC 1 cut(s) 41
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 2 cut(s) 181, 206
Tsp45I GTSAC 1 cut(s) 73
TspGWI ACGGA 1 cut(s) 124
XcmI CCANNNNNNNNNTGG 1 cut(s) 43
XhoI CTCGAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.