pycom13g17110

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002729
Physical Location & Seq
Reverse (-)
12835045 .. 12838622
3578 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1002 bp
ATGGATTTAGGAAGCGAGGAAACGTCGTCGTTCCTGTCTGATTTCCTGGAAAAGTGTGGTGGCTATGCTGTCCTTGACGGCGGTTTTGCTACAGAGCTTGAACGGCATGGAGCTGACCTCAACGACCCTCTTTGGAGCGCCAAATGCCTTATCACTTCCCCTCACCTTGTTAGAAGGGTACACTTGGACTACCTTGACGCCGGTGCAAACATCATACTAACCGCATCTTATCAGGCCACCATTCAGGGCTTTGAGGCCAAAGGTTTCTCTAAGGAAGAAGCCAAAGATTTGCTAAGGAAAAGTGTTGAAATTGCAATTGAGGCGCGTGAAATTTACTATGATAAGTTTCAATCAAGACGTCCAGTTCTGGTTGCAGCATCTATCGGCAGCTATGGCGCTTACTTGGCTGATGGTTCCGAATATAGTGGGAACTATGGAGATGCGATAACTGTAGAAACATTGAAGGATTTCCACAGGGAAAGGGTACAGATTCTGGCCAATTCTGGTGCTGATCTGATCGCATTTGAGACGATTCCAAATAAGATAGAAGCAAAGGCATATGCTGAGCTTCTTGATGAAGAAGGAATAGAAATTCCAGCCTGGTTTTCCTTCGCTTCGAAGGATGGAATCAATGTGGTCAGCGGGGATTCCATTTTAGAATGTGCCTCCATTGCAGATTCATGCAAGAACGTTGTTGCTGTTGGAATCAACTGCACACCTCCTAGATTTATCTACGGACTGGTTTCATCTATTCGGAATGTTACAAACAAACCAATAGTGATATACCCTAACAGTGGTGAGGCTTATGATGGTCAGACCAAGCAATGGGTGAAATCGACTGGTGTGGTTGATGAAGAGTTTGCAGACATAGCCATAGACAAGTGGTGTGAGGCTGGGGCTTCTCTATTTGGAGGCTGCTGCAGGACTACTCCAAATACCATCAGAGCCATATCTAAAGCTCTCTCCAACAAGTCTTCTTCCATCGTTAATGATGATGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

334

Amino Acids

36.09

Weight (kDa)

4.7

Isoelectric Point (pI)

40.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 24 - 321 1.6e-83 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 361
AccB7I CCANNNNNTGG 1 cut(s) 825
AccII CGCG 1 cut(s) 325
AciI CCGC 3 cut(s) 81, 222, 642
AclI AACGTT 1 cut(s) 690
AcoI YGGCCR 1 cut(s) 495
AcsI RAATTY 2 cut(s) 330, 591
AcyI GRCGYC 2 cut(s) 198, 358
AfaI GTAC 2 cut(s) 180, 486
AfiI CCNNNNNNNGG 2 cut(s) 794, 825
AgsI TTSAA 4 cut(s) 101, 308, 350, 463
AjnI CCWGG 2 cut(s) 45, 599
AluBI AGCT 5 cut(s) 97, 113, 390, 568, 959
AluI AGCT 5 cut(s) 97, 113, 390, 568, 959
Alw26I GTCTC 1 cut(s) 521
AlwNI CAGNNNCTG 1 cut(s) 493
AoxI GGCC 3 cut(s) 234, 255, 495
ApeKI GCWGC 4 cut(s) 374, 387, 915, 918
ApoI RAATTY 2 cut(s) 330, 591
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
Asp700I GAANNNNTTC 1 cut(s) 467
AspLEI GCGC 3 cut(s) 140, 325, 398
AsuHPI GGTGA 3 cut(s) 155, 809, 841
AsuII TTCGAA 1 cut(s) 617
BalI TGGCCA 1 cut(s) 497
BbsI GAAGAC 1 cut(s) 966
BbvI GCAGC 4 cut(s) 386, 399, 902, 905
BccI CCATC 5 cut(s) 404, 617, 803, 947, 989
BceAI ACGGC 2 cut(s) 94, 119
BciT130I CCWGG 2 cut(s) 47, 601
BcoDI GTCTC 1 cut(s) 521
BfaI CTAG 1 cut(s) 723
BfmI CTRYAG 3 cut(s) 90, 450, 919
BfoI RGCGCY 2 cut(s) 141, 399
BisI GCNGC 4 cut(s) 375, 388, 916, 919
BlpI GCTNAGC 1 cut(s) 564
BlsI GCNGC 4 cut(s) 376, 389, 917, 920
Bme1390I CCNGG 2 cut(s) 47, 601
BmiI GGNNCC 1 cut(s) 415
BmrFI CCNGG 2 cut(s) 47, 601
BmsI GCATC 4 cut(s) 233, 386, 430, 985
BpiI GAAGAC 1 cut(s) 966
BplI GAGNNNNNCTC 2 cut(s) 102, 134
Bpu10I CCTNAGC 1 cut(s) 293
Bpu1102I GCTNAGC 1 cut(s) 564
Bpu14I TTCGAA 1 cut(s) 617
BsaHI GRCGYC 2 cut(s) 198, 358
Bsc4I CCNNNNNNNGG 2 cut(s) 794, 825
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 3 cut(s) 362, 744, 844
Bse3DI GCAATG 2 cut(s) 669, 830
BseBI CCWGG 2 cut(s) 47, 601
BseGI GGATG 1 cut(s) 628
BseLI CCNNNNNNNGG 2 cut(s) 794, 825
BseMI GCAATG 2 cut(s) 669, 830
BseMII CTCAG 1 cut(s) 555
BseNI ACTGG 3 cut(s) 362, 744, 844
BseXI GCAGC 4 cut(s) 386, 399, 902, 905
BseYI CCCAGC 1 cut(s) 893
BsgI GTGCAG 1 cut(s) 697
Bsh1236I CGCG 1 cut(s) 325
BshFI GGCC 3 cut(s) 236, 257, 497
BsiSI CCGG 1 cut(s) 201
BslI CCNNNNNNNGG 2 cut(s) 794, 825
BsmAI GTCTC 1 cut(s) 521
BsmBI CGTCTC 1 cut(s) 521
BsnI GGCC 3 cut(s) 236, 257, 497
Bsp119I TTCGAA 1 cut(s) 617
Bsp143I GATC 2 cut(s) 511, 516
Bsp1720I GCTNAGC 1 cut(s) 564
BspACI CCGC 3 cut(s) 81, 222, 642
BspANI GGCC 3 cut(s) 236, 257, 497
BspCNI CTCAG 1 cut(s) 556
BspFNI CGCG 1 cut(s) 325
BspLI GGNNCC 1 cut(s) 415
BspMAI CTGCAG 1 cut(s) 923
BspT104I TTCGAA 1 cut(s) 617
BsrDI GCAATG 2 cut(s) 669, 830
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 3 cut(s) 362, 744, 844
BssAI RCCGGY 1 cut(s) 200
BssMI GATC 2 cut(s) 511, 516
BssNI GRCGYC 2 cut(s) 198, 358
Bst2UI CCWGG 2 cut(s) 47, 601
Bst4CI ACNGT 2 cut(s) 451, 794
Bst6I CTCTTC 1 cut(s) 849
BstACI GRCGYC 2 cut(s) 198, 358
BstBI TTCGAA 1 cut(s) 617
BstDEI CTNAG 3 cut(s) 270, 293, 564
BstF5I GGATG 1 cut(s) 628
BstFNI CGCG 1 cut(s) 325
BstH2I RGCGCY 2 cut(s) 141, 399
BstHHI GCGC 3 cut(s) 140, 325, 398
BstKTI GATC 2 cut(s) 514, 519
BstMAI GTCTC 1 cut(s) 521
BstMBI GATC 2 cut(s) 511, 516
BstMWI GCNNNNNNNGC 7 cut(s) 103, 144, 320, 393, 404, 671, 869
BstNI CCWGG 2 cut(s) 47, 601
BstSCI CCNGG 2 cut(s) 45, 599
BstSFI CTRYAG 3 cut(s) 90, 450, 919
BstUI CGCG 1 cut(s) 325
BstV1I GCAGC 4 cut(s) 386, 399, 902, 905
BstV2I GAAGAC 1 cut(s) 966
BsuRI GGCC 3 cut(s) 236, 257, 497
BtsCI GGATG 1 cut(s) 628
BtsIMutI CAGTG 1 cut(s) 799
CaiI CAGNNNCTG 1 cut(s) 493
CfoI GCGC 3 cut(s) 140, 325, 398
Cfr10I RCCGGY 1 cut(s) 200
CseI GACGC 1 cut(s) 206
Csp6I GTAC 2 cut(s) 179, 485
CviAII CATG 2 cut(s) 107, 681
CviQI GTAC 2 cut(s) 179, 485
DdeI CTNAG 3 cut(s) 270, 293, 564
DpnI GATC 2 cut(s) 513, 518
DpnII GATC 2 cut(s) 511, 516
EaeI YGGCCR 1 cut(s) 495
Eam1104I CTCTTC 1 cut(s) 849
EarI CTCTTC 1 cut(s) 849
EcoRII CCWGG 2 cut(s) 45, 599
Esp3I CGTCTC 1 cut(s) 521
FaeI CATG 2 cut(s) 110, 684
FatI CATG 2 cut(s) 106, 680
FauI CCCGC 1 cut(s) 635
FauNDI CATATG 1 cut(s) 559
Fnu4HI GCNGC 4 cut(s) 375, 388, 916, 919
FokI GGATG 1 cut(s) 635
Fsp4HI GCNGC 4 cut(s) 375, 388, 916, 919
FspBI CTAG 1 cut(s) 723
GlaI GCGC 3 cut(s) 139, 324, 397
GluI GCNGC 4 cut(s) 375, 388, 916, 919
GsaI CCCAGC 1 cut(s) 897
HaeII RGCGCY 2 cut(s) 141, 399
HaeIII GGCC 3 cut(s) 236, 257, 497
HapII CCGG 1 cut(s) 201
HgaI GACGC 1 cut(s) 206
HhaI GCGC 3 cut(s) 140, 325, 398
Hin1I GRCGYC 2 cut(s) 198, 358
Hin1II CATG 2 cut(s) 110, 684
Hin6I GCGC 3 cut(s) 138, 323, 396
HinP1I GCGC 3 cut(s) 138, 323, 396
HinfI GANTC 6 cut(s) 490, 532, 627, 647, 677, 705
HpaII CCGG 1 cut(s) 201
HphI GGTGA 3 cut(s) 155, 809, 841
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 6 cut(s) 40, 418, 516, 756, 816, 944
Hpy188III TCNNGA 2 cut(s) 354, 572
Hpy8I GTNNAC 1 cut(s) 181
Hpy99I CGWCG 2 cut(s) 28, 31
HpyAV CCTTC 5 cut(s) 168, 457, 575, 613, 619
HpyCH4III ACNGT 2 cut(s) 451, 794
HpyCH4IV ACGT 3 cut(s) 23, 358, 690
HpyCH4V TGCA 8 cut(s) 206, 314, 374, 674, 684, 714, 863, 921
HpyF10VI GCNNNNNNNGC 7 cut(s) 103, 144, 320, 393, 404, 671, 869
HpyF3I CTNAG 3 cut(s) 270, 293, 564
HpySE526I ACGT 3 cut(s) 23, 358, 690
Hsp92I GRCGYC 2 cut(s) 198, 358
Hsp92II CATG 2 cut(s) 110, 684
HspAI GCGC 3 cut(s) 138, 323, 396
Kzo9I GATC 2 cut(s) 511, 516
LmnI GCTCC 2 cut(s) 110, 135
Lsp1109I GCAGC 4 cut(s) 386, 399, 902, 905
LweI GCATC 4 cut(s) 233, 386, 430, 985
MaeI CTAG 1 cut(s) 723
MaeII ACGT 3 cut(s) 23, 358, 690
MaeIII GTNAC 1 cut(s) 760
MalI GATC 2 cut(s) 513, 518
MboI GATC 2 cut(s) 511, 516
MboII GAAGA 5 cut(s) 287, 590, 866, 966, 969
MfeI CAATTG 1 cut(s) 315
MlsI TGGCCA 1 cut(s) 497
MluCI AATT 5 cut(s) 309, 315, 330, 499, 591
MluNI TGGCCA 1 cut(s) 497
MmeI TCCRAC 2 cut(s) 682, 990
Mox20I TGGCCA 1 cut(s) 497
MroXI GAANNNNTTC 1 cut(s) 467
MscI TGGCCA 1 cut(s) 497
MseI TTAA 1 cut(s) 987
Msp20I TGGCCA 1 cut(s) 497
MspA1I CMGCKG 1 cut(s) 642
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 2 cut(s) 47, 601
MunI CAATTG 1 cut(s) 315
MvaI CCWGG 2 cut(s) 47, 601
MvnI CGCG 1 cut(s) 325
MwoI GCNNNNNNNGC 7 cut(s) 103, 144, 320, 393, 404, 671, 869
NdeI CATATG 1 cut(s) 559
NdeII GATC 2 cut(s) 511, 516
NlaIII CATG 2 cut(s) 110, 684
NlaIV GGNNCC 1 cut(s) 415
NspV TTCGAA 1 cut(s) 617
PdmI GAANNNNTTC 1 cut(s) 467
PfeI GAWTC 6 cut(s) 490, 532, 627, 647, 677, 705
PflMI CCANNNNNTGG 1 cut(s) 825
PfoI TCCNGGA 1 cut(s) 45
PkrI GCNGC 4 cut(s) 376, 389, 917, 920
Psp1406I AACGTT 1 cut(s) 690
Psp6I CCWGG 2 cut(s) 45, 599
PspFI CCCAGC 1 cut(s) 893
PspGI CCWGG 2 cut(s) 45, 599
PspN4I GGNNCC 1 cut(s) 415
PstI CTGCAG 1 cut(s) 923
PstNI CAGNNNCTG 1 cut(s) 493
RsaI GTAC 2 cut(s) 180, 486
RsaNI GTAC 2 cut(s) 179, 485
SaqAI TTAA 1 cut(s) 987
SatI GCNGC 4 cut(s) 375, 388, 916, 919
Sau3AI GATC 2 cut(s) 511, 516
ScrFI CCNGG 2 cut(s) 47, 601
SfaNI GCATC 4 cut(s) 233, 386, 430, 985
SfcI CTRYAG 3 cut(s) 90, 450, 919
SfuI TTCGAA 1 cut(s) 617
SgrAI CRCCGGYG 1 cut(s) 200
Sse9I AATT 5 cut(s) 309, 315, 330, 499, 591
SsiI CCGC 3 cut(s) 81, 222, 642
SspMI CTAG 1 cut(s) 723
StyD4I CCNGG 2 cut(s) 45, 599
TaaI ACNGT 2 cut(s) 451, 794
TaiI ACGT 3 cut(s) 26, 361, 693
TaqI TCGA 2 cut(s) 617, 836
TasI AATT 5 cut(s) 309, 315, 330, 499, 591
TfiI GAWTC 6 cut(s) 490, 532, 627, 647, 677, 705
Tru1I TTAA 1 cut(s) 987
Tru9I TTAA 1 cut(s) 987
TscAI CASTG 1 cut(s) 799
TseI GCWGC 4 cut(s) 374, 387, 915, 918
TspDTI ATGAA 4 cut(s) 591, 669, 735, 867
TspGWI ACGGA 1 cut(s) 750
TspRI CASTG 1 cut(s) 799
Van91I CCANNNNNTGG 1 cut(s) 825
XapI RAATTY 2 cut(s) 330, 591
XmnI GAANNNNTTC 1 cut(s) 467
XspI CTAG 1 cut(s) 723
ZraI GACGTC 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.