pycom16g10090

Belongs to the universal ribosomal protein uL22 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001814
Physical Location & Seq
Reverse (-)
6859520 .. 6862038
2519 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 663 bp
ATGAAGCCCAAAGAAGCCCAATTGAGGCACAAGTCCCACAAGAACTCGCCTCTTCTGCGTCTAAGCAAAAGCAAGCGTAAACCCTTCACTGAGGCAGAAGCTCCATCGGCCGCCATGGTGAAGTACTCCAGAGAGCCCGACAATATCACCAAGTCTTGCAAAGCAAGAGGTTCTGACCTGAGGGTTCATTTCAAGAACACAAGGGAAACTGCGTTTTCTATCAGAAAGATGCGCTTGGACAAGGCAAAGAGTTATTTGGAGGATGTTCTGGCTCACAAACAGGCCATTCCCTTCCGTCGTTTCTGCCGTGGAGTTGGGCGTACTGCCCAGGCAAAGAACAGGCATTCCAATGGGCAAGGACGCTGGCCTGTCAAGTCTGCCAAGTTCATATTAGATTTGCTTAAGAATGCTGAGAGCAATGCTGAAGTGAAGGGTTTGGATGTTGATTCCCTAATCGTATCTCACATCCAGGTCAACCAAGCACAGAAGCAAAGGCGCCGAACCTACAGGGCTCACGGAAGAATCAACCCCTACATGTCATCGCCCTGCCATATTGAGCTGATCCTTTCCGAGAAGGAAGAACCCGTCAAGAAAGAGCCCGAGAGCCAGTTGGCAACAAGCAAATCGAAGAAGGCTGCTCTTAGGAGCGGTGCTTCCTCTTAG

Protein Analysis

221

Amino Acids

24.95

Weight (kDa)

10.43

Isoelectric Point (pI)

64.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L22 PF00237 55 - 190 6.1e-33 Ribosomal protein L22p/L17e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 495
AccBSI CCGCTC 1 cut(s) 648
AciI CCGC 2 cut(s) 111, 648
AclWI GGATC 1 cut(s) 556
AcoI YGGCCR 1 cut(s) 108
AcuI CTGAAG 1 cut(s) 444
AcyI GRCGYC 1 cut(s) 496
AfaI GTAC 2 cut(s) 125, 322
AfiI CCNNNNNNNGG 1 cut(s) 24
AflII CTTAAG 1 cut(s) 401
AflIII ACRYGT 1 cut(s) 534
AgsI TTSAA 1 cut(s) 193
AjnI CCWGG 2 cut(s) 327, 468
AloI GAACNNNNNNTCC 2 cut(s) 329, 361
AluBI AGCT 2 cut(s) 101, 559
AluI AGCT 2 cut(s) 101, 559
AlwI GGATC 1 cut(s) 556
Ama87I CYCGRG 1 cut(s) 599
AoxI GGCC 3 cut(s) 108, 282, 365
ApeKI GCWGC 1 cut(s) 635
AspLEI GCGC 2 cut(s) 234, 498
AsuHPI GGTGA 2 cut(s) 130, 139
AvaI CYCGRG 1 cut(s) 599
AxyI CCTNAGG 1 cut(s) 179
BanI GGYRCC 1 cut(s) 495
BanII GRGCYC 3 cut(s) 138, 514, 600
BbvI GCAGC 1 cut(s) 622
BccI CCATC 1 cut(s) 112
BceAI ACGGC 1 cut(s) 291
BciT130I CCWGG 2 cut(s) 329, 470
BfmI CTRYAG 1 cut(s) 505
BfoI RGCGCY 1 cut(s) 499
BfrI CTTAAG 1 cut(s) 401
BisI GCNGC 2 cut(s) 111, 636
BlsI GCNGC 2 cut(s) 112, 637
BmcAI AGTACT 1 cut(s) 125
Bme1390I CCNGG 2 cut(s) 329, 470
BmeT110I CYCGRG 1 cut(s) 599
BmiI GGNNCC 1 cut(s) 497
BmrFI CCNGG 2 cut(s) 329, 470
BmsI GCATC 1 cut(s) 219
BpmI CTGGAG 1 cut(s) 112
BsaHI GRCGYC 1 cut(s) 496
BsaJI CCNNGG 3 cut(s) 114, 307, 327
Bsc4I CCNNNNNNNGG 1 cut(s) 24
Bse1I ACTGG 1 cut(s) 607
Bse21I CCTNAGG 1 cut(s) 179
Bse3DI GCAATG 1 cut(s) 424
BseBI CCWGG 2 cut(s) 329, 470
BseDI CCNNGG 3 cut(s) 114, 307, 327
BseGI GGATG 3 cut(s) 268, 445, 465
BseLI CCNNNNNNNGG 1 cut(s) 24
BseMI GCAATG 1 cut(s) 424
BseMII CTCAG 3 cut(s) 81, 170, 402
BseNI ACTGG 1 cut(s) 607
BseX3I CGGCCG 1 cut(s) 108
BseXI GCAGC 1 cut(s) 622
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 3 cut(s) 110, 284, 367
BshNI GGYRCC 1 cut(s) 495
BsiEI CGRYCG 1 cut(s) 111
BsiHKCI CYCGRG 1 cut(s) 599
BslFI GGGAC 1 cut(s) 19
BslI CCNNNNNNNGG 1 cut(s) 24
BsmFI GGGAC 1 cut(s) 19
BsmI GAATGC 2 cut(s) 343, 412
BsnI GGCC 3 cut(s) 110, 284, 367
BsoBI CYCGRG 1 cut(s) 599
Bsp1286I GDGCHC 3 cut(s) 138, 514, 600
Bsp143I GATC 1 cut(s) 561
Bsp19I CCATGG 1 cut(s) 114
BspACI CCGC 2 cut(s) 111, 648
BspANI GGCC 3 cut(s) 110, 284, 367
BspCNI CTCAG 3 cut(s) 82, 171, 403
BspLI GGNNCC 1 cut(s) 497
BspPI GGATC 1 cut(s) 556
BspT107I GGYRCC 1 cut(s) 495
BspTI CTTAAG 1 cut(s) 401
BsrBI CCGCTC 1 cut(s) 648
BsrDI GCAATG 1 cut(s) 424
BsrI ACTGG 1 cut(s) 607
BssECI CCNNGG 3 cut(s) 114, 307, 327
BssMI GATC 1 cut(s) 561
BssNI GRCGYC 1 cut(s) 496
BssT1I CCWWGG 1 cut(s) 114
Bst2UI CCWGG 2 cut(s) 329, 470
Bst6I CTCTTC 1 cut(s) 57
BstACI GRCGYC 1 cut(s) 496
BstAFI CTTAAG 1 cut(s) 401
BstC8I GCNNGC 2 cut(s) 74, 365
BstDEI CTNAG 6 cut(s) 62, 90, 179, 411, 641, 660
BstDSI CCRYGG 2 cut(s) 114, 307
BstF5I GGATG 3 cut(s) 268, 445, 465
BstH2I RGCGCY 1 cut(s) 499
BstHHI GCGC 2 cut(s) 234, 498
BstKTI GATC 1 cut(s) 564
BstMBI GATC 1 cut(s) 561
BstMCI CGRYCG 1 cut(s) 111
BstMWI GCNNNNNNNGC 2 cut(s) 55, 107
BstNI CCWGG 2 cut(s) 329, 470
BstNSI RCATGY 1 cut(s) 538
BstSCI CCNGG 2 cut(s) 327, 468
BstSFI CTRYAG 1 cut(s) 505
BstV1I GCAGC 1 cut(s) 622
BstZI CGGCCG 1 cut(s) 108
Bsu36I CCTNAGG 1 cut(s) 179
BsuRI GGCC 3 cut(s) 110, 284, 367
BtgI CCRYGG 2 cut(s) 114, 307
BtgZI GCGATG 1 cut(s) 525
BtsCI GGATG 3 cut(s) 268, 445, 465
BtsIMutI CAGTG 1 cut(s) 87
Cac8I GCNNGC 2 cut(s) 74, 365
CfoI GCGC 2 cut(s) 234, 498
CseI GACGC 2 cut(s) 47, 369
Csp6I GTAC 2 cut(s) 124, 321
CviAII CATG 2 cut(s) 115, 535
CviQI GTAC 2 cut(s) 124, 321
DdeI CTNAG 6 cut(s) 62, 90, 179, 411, 641, 660
DinI GGCGCC 1 cut(s) 497
DpnI GATC 1 cut(s) 563
DpnII GATC 1 cut(s) 561
EaeI YGGCCR 1 cut(s) 108
EagI CGGCCG 1 cut(s) 108
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
EclXI CGGCCG 1 cut(s) 108
Eco130I CCWWGG 1 cut(s) 114
Eco24I GRGCYC 3 cut(s) 138, 514, 600
Eco52I CGGCCG 1 cut(s) 108
Eco57I CTGAAG 1 cut(s) 444
Eco81I CCTNAGG 1 cut(s) 179
Eco88I CYCGRG 1 cut(s) 599
EcoRII CCWGG 2 cut(s) 327, 468
EcoT14I CCWWGG 1 cut(s) 114
EcoT38I GRGCYC 3 cut(s) 138, 514, 600
EgeI GGCGCC 1 cut(s) 497
EheI GGCGCC 1 cut(s) 497
ErhI CCWWGG 1 cut(s) 114
FaeI CATG 2 cut(s) 118, 538
FaiI YATR 4 cut(s) 116, 389, 536, 552
FalI AAGNNNNNCTT 2 cut(s) 218, 250
FaqI GGGAC 1 cut(s) 19
FatI CATG 2 cut(s) 114, 534
Fnu4HI GCNGC 2 cut(s) 111, 636
FokI GGATG 3 cut(s) 275, 452, 452
FriOI GRGCYC 3 cut(s) 138, 514, 600
Fsp4HI GCNGC 2 cut(s) 111, 636
GlaI GCGC 2 cut(s) 233, 497
GluI GCNGC 2 cut(s) 111, 636
GsuI CTGGAG 1 cut(s) 112
HaeII RGCGCY 1 cut(s) 499
HaeIII GGCC 3 cut(s) 110, 284, 367
HgaI GACGC 2 cut(s) 47, 369
HhaI GCGC 2 cut(s) 234, 498
Hin1I GRCGYC 1 cut(s) 496
Hin1II CATG 2 cut(s) 118, 538
Hin6I GCGC 2 cut(s) 232, 496
HinP1I GCGC 2 cut(s) 232, 496
HincII GTYRAC 1 cut(s) 475
HindII GTYRAC 1 cut(s) 475
HinfI GANTC 2 cut(s) 446, 522
HphI GGTGA 2 cut(s) 130, 139
Hpy166II GTNNAC 2 cut(s) 80, 475
Hpy188I TCNGA 3 cut(s) 175, 224, 571
Hpy188III TCNNGA 3 cut(s) 129, 193, 589
Hpy8I GTNNAC 2 cut(s) 80, 475
Hpy99I CGWCG 1 cut(s) 300
HpyAV CCTTC 5 cut(s) 94, 301, 424, 568, 625
HpyCH4V TGCA 1 cut(s) 159
HpyF10VI GCNNNNNNNGC 2 cut(s) 55, 107
HpyF3I CTNAG 6 cut(s) 62, 90, 179, 411, 641, 660
Hsp92I GRCGYC 1 cut(s) 496
Hsp92II CATG 2 cut(s) 118, 538
HspAI GCGC 2 cut(s) 232, 496
KasI GGCGCC 1 cut(s) 495
Kzo9I GATC 1 cut(s) 561
LmnI GCTCC 2 cut(s) 106, 645
Lsp1109I GCAGC 1 cut(s) 622
LweI GCATC 1 cut(s) 219
MalI GATC 1 cut(s) 563
MbiI CCGCTC 1 cut(s) 648
MboI GATC 1 cut(s) 561
MboII GAAGA 4 cut(s) 44, 531, 590, 640
MfeI CAATTG 1 cut(s) 20
MhlI GDGCHC 3 cut(s) 138, 514, 600
MluCI AATT 1 cut(s) 20
Mly113I GGCGCC 1 cut(s) 496
MnlI CCTC 6 cut(s) 18, 60, 85, 161, 174, 253
MseI TTAA 1 cut(s) 402
MslI CAYNNNNRTG 1 cut(s) 348
MspCI CTTAAG 1 cut(s) 401
MspR9I CCNGG 2 cut(s) 329, 470
MunI CAATTG 1 cut(s) 20
Mva1269I GAATGC 2 cut(s) 343, 412
MvaI CCWGG 2 cut(s) 329, 470
MwoI GCNNNNNNNGC 2 cut(s) 55, 107
NarI GGCGCC 1 cut(s) 496
NcoI CCATGG 1 cut(s) 114
NdeII GATC 1 cut(s) 561
NlaIII CATG 2 cut(s) 118, 538
NlaIV GGNNCC 1 cut(s) 497
NspI RCATGY 1 cut(s) 538
PciI ACATGT 1 cut(s) 534
PcsI WCGNNNNNNNCGW 1 cut(s) 304
PctI GAATGC 2 cut(s) 343, 412
PfeI GAWTC 2 cut(s) 446, 522
PkrI GCNGC 2 cut(s) 112, 637
PluTI GGCGCC 1 cut(s) 499
PscI ACATGT 1 cut(s) 534
Psp6I CCWGG 2 cut(s) 327, 468
PspGI CCWGG 2 cut(s) 327, 468
PspN4I GGNNCC 1 cut(s) 497
RsaI GTAC 2 cut(s) 125, 322
RsaNI GTAC 2 cut(s) 124, 321
RseI CAYNNNNRTG 1 cut(s) 348
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 2 cut(s) 111, 636
Sau3AI GATC 1 cut(s) 561
ScaI AGTACT 1 cut(s) 125
ScrFI CCNGG 2 cut(s) 329, 470
SduI GDGCHC 3 cut(s) 138, 514, 600
SetI ASST 6 cut(s) 103, 172, 180, 474, 506, 561
SfaNI GCATC 1 cut(s) 219
SfcI CTRYAG 1 cut(s) 505
SfoI GGCGCC 1 cut(s) 497
SmiMI CAYNNNNRTG 1 cut(s) 348
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
Sse9I AATT 1 cut(s) 20
SsiI CCGC 2 cut(s) 111, 648
SspDI GGCGCC 1 cut(s) 495
StyD4I CCNGG 2 cut(s) 327, 468
StyI CCWWGG 1 cut(s) 114
TaqI TCGA 1 cut(s) 626
TasI AATT 1 cut(s) 20
TatI WGTACW 1 cut(s) 123
TauI GCSGC 1 cut(s) 113
TfiI GAWTC 2 cut(s) 446, 522
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TscAI CASTG 1 cut(s) 94
TseI GCWGC 1 cut(s) 635
TspDTI ATGAA 3 cut(s) 17, 176, 376
TspGWI ACGGA 2 cut(s) 284, 531
TspRI CASTG 1 cut(s) 94
Vha464I CTTAAG 1 cut(s) 401
XceI RCATGY 1 cut(s) 538
ZrmI AGTACT 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.