pycom16g21070

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0002774
Physical Location & Seq
Forward (+)
19227567 .. 19228403
837 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 837 bp
ATGAAGCTTGCTCTCGAAGTATCCTCACTCATTAGCATTACTTGCCCACAAAGCAAATCAAAAACCAAAATCCCAAAAGAAATCCCCCACATTTCGAATCCCACCTCCTATTTAATCCCACCCATCTCTCTGTTTTTCGTTGCCATTAAAACCCCAGAAAGCTTTGCTGTTTTTGTTTTGGTTATGACAAACGGTTCTCAAAGCGGCAATGCGTCAAACACATTCGAGGCATTCTACGAGGGCTGGCTGGTCCAGCAAGGACACTTCCTCGATGAGCTCTTATCGGCCCAACAGACGATCGACGAGGCCCGAGATGACGACCTCCGAGATTTGGTGTCTCGGGTTCTCTTCCATTACCAGCAGTATTACGACGAGAAATTGCAACTTGGACAGCGAGATGTTTTACTGGTTTTCTCGCCGACGTGGTATACTTCCTACGAAAGGAGCCTGCTTTGGATCGCCGGGTACAAACCGAGTATCGTATTCCGGCTCGTGACCGAGTCGGTGTCCGATTTGAGCGACCAGCTGCAGGTCAGGATGGCCCGGCTGAGGGAGGCGACCTGGGTCGAGGAGCGCGCGCTCAACAACAAGCTCGCCAAGATTCACGAGAGCGTGGCGGCACCGCTGTTCATGGATGCGGTGAGGCGGTACGGGAGGGCCGGCCACGGGGAGATCGTGGCGGACGACGCGGTGATCAAGTCGCTTAAGTCGGCATTGGAGACCGTGGTGGAGAATGCCAACTTGCTGAGGACGACGATGGCGACGAAGCTGGTGGATTTGCTGAGCTCGGGGCAGGCAGTGAAATTTTTGGCTAATAATTTTGATTATGGATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DOG1

Protein Analysis

279

Amino Acids

31.37

Weight (kDa)

5.79

Isoelectric Point (pI)

35.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DOG1 PF14144 90 - 166 3.6e-22 Seed dormancy control
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 520
AccB1I GGYRCC 1 cut(s) 619
AccI GTMKAC 1 cut(s) 428
AccII CGCG 3 cut(s) 576, 578, 689
AciI CCGC 7 cut(s) 204, 617, 623, 638, 646, 680, 689
AclWI GGATC 1 cut(s) 464
AcoI YGGCCR 1 cut(s) 661
AcsI RAATTY 1 cut(s) 803
AfaI GTAC 2 cut(s) 467, 650
AfiI CCNNNNNNNGG 6 cut(s) 331, 441, 529, 549, 550, 666
AflII CTTAAG 1 cut(s) 704
AjiI CACGTC 1 cut(s) 423
AjnI CCWGG 1 cut(s) 560
AluBI AGCT 7 cut(s) 7, 162, 277, 526, 592, 769, 786
AluI AGCT 7 cut(s) 7, 162, 277, 526, 592, 769, 786
Alw21I GWGCWC 2 cut(s) 279, 788
Alw26I GTCTC 2 cut(s) 342, 713
AlwI GGATC 1 cut(s) 464
Ama87I CYCGRG 3 cut(s) 309, 339, 787
AoxI GGCC 5 cut(s) 285, 306, 540, 657, 661
ApeKI GCWGC 1 cut(s) 526
ApoI RAATTY 1 cut(s) 803
AspLEI GCGC 3 cut(s) 576, 578, 580
AspS9I GGNCC 5 cut(s) 250, 286, 307, 541, 657
AsuC2I CCSGG 2 cut(s) 463, 544
AsuHPI GGTGA 2 cut(s) 652, 703
AsuII TTCGAA 1 cut(s) 95
AvaI CYCGRG 3 cut(s) 309, 339, 787
AvaII GGWCC 1 cut(s) 250
BanI GGYRCC 1 cut(s) 619
BanII GRGCYC 2 cut(s) 279, 788
BauI CACGAG 2 cut(s) 491, 605
Bbv12I GWGCWC 2 cut(s) 279, 788
BbvCI CCTCAGC 2 cut(s) 548, 746
BbvI GCAGC 1 cut(s) 513
BccI CCATC 4 cut(s) 131, 532, 751, 825
BciT130I CCWGG 1 cut(s) 562
BciVI GTATCC 1 cut(s) 31
BclI TGATCA 1 cut(s) 693
BcnI CCSGG 2 cut(s) 463, 544
BcoDI GTCTC 2 cut(s) 342, 713
BfmI CTRYAG 1 cut(s) 527
BfrI CTTAAG 1 cut(s) 704
BfuAI ACCTGC 1 cut(s) 520
BfuI GTATCC 1 cut(s) 31
BisI GCNGC 3 cut(s) 205, 527, 618
BlpI GCTNAGC 1 cut(s) 782
BlsI GCNGC 3 cut(s) 206, 528, 619
Bme1390I CCNGG 3 cut(s) 463, 544, 562
Bme18I GGWCC 1 cut(s) 250
BmeT110I CYCGRG 3 cut(s) 309, 339, 787
BmgBI CACGTC 1 cut(s) 423
BmgT120I GGNCC 5 cut(s) 250, 286, 307, 541, 657
BmiI GGNNCC 2 cut(s) 446, 621
BmrFI CCNGG 3 cut(s) 463, 544, 562
BmsI GCATC 1 cut(s) 625
BoxI GACNNNNGTC 1 cut(s) 563
Bpu10I CCTNAGC 2 cut(s) 548, 746
Bpu1102I GCTNAGC 1 cut(s) 782
Bpu14I TTCGAA 1 cut(s) 95
BpuMI CCSGG 2 cut(s) 463, 544
BsaI GGTCTC 1 cut(s) 713
BsaJI CCNNGG 3 cut(s) 561, 664, 723
Bsc4I CCNNNNNNNGG 6 cut(s) 331, 441, 529, 549, 550, 666
Bse118I RCCGGY 1 cut(s) 659
Bse1I ACTGG 1 cut(s) 411
Bse3DI GCAATG 1 cut(s) 214
BseBI CCWGG 1 cut(s) 562
BseDI CCNNGG 3 cut(s) 561, 664, 723
BseGI GGATG 3 cut(s) 543, 640, 836
BseLI CCNNNNNNNGG 6 cut(s) 331, 441, 529, 549, 550, 666
BseMI GCAATG 1 cut(s) 214
BseMII CTCAG 3 cut(s) 539, 737, 773
BseNI ACTGG 1 cut(s) 411
BsePI GCGCGC 2 cut(s) 574, 576
BseRI GAGGAG 1 cut(s) 584
BseXI GCAGC 1 cut(s) 513
Bsh1236I CGCG 3 cut(s) 576, 578, 689
Bsh1285I CGRYCG 1 cut(s) 300
BshFI GGCC 5 cut(s) 287, 308, 542, 659, 663
BshNI GGYRCC 1 cut(s) 619
BsiEI CGRYCG 1 cut(s) 300
BsiHKAI GWGCWC 2 cut(s) 279, 788
BsiHKCI CYCGRG 3 cut(s) 309, 339, 787
BsiSI CCGG 4 cut(s) 462, 487, 544, 660
BslI CCNNNNNNNGG 6 cut(s) 331, 441, 529, 549, 550, 666
BsmAI GTCTC 2 cut(s) 342, 713
BsmI GAATGC 2 cut(s) 230, 739
BsnI GGCC 5 cut(s) 287, 308, 542, 659, 663
Bso31I GGTCTC 1 cut(s) 713
BsoBI CYCGRG 3 cut(s) 309, 339, 787
Bsp119I TTCGAA 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 279, 788
Bsp143I GATC 4 cut(s) 297, 456, 672, 693
Bsp1720I GCTNAGC 1 cut(s) 782
BspACI CCGC 7 cut(s) 204, 617, 623, 638, 646, 680, 689
BspANI GGCC 5 cut(s) 287, 308, 542, 659, 663
BspCNI CTCAG 3 cut(s) 540, 738, 774
BspFNI CGCG 3 cut(s) 576, 578, 689
BspLI GGNNCC 2 cut(s) 446, 621
BspMAI CTGCAG 1 cut(s) 531
BspMI ACCTGC 1 cut(s) 520
BspPI GGATC 1 cut(s) 464
BspT104I TTCGAA 1 cut(s) 95
BspT107I GGYRCC 1 cut(s) 619
BspTI CTTAAG 1 cut(s) 704
BspTNI GGTCTC 1 cut(s) 713
BsrDI GCAATG 1 cut(s) 214
BsrFI RCCGGY 1 cut(s) 659
BsrI ACTGG 1 cut(s) 411
BssAI RCCGGY 1 cut(s) 659
BssECI CCNNGG 3 cut(s) 561, 664, 723
BssHII GCGCGC 2 cut(s) 574, 576
BssMI GATC 4 cut(s) 297, 456, 672, 693
BssNAI GTATAC 1 cut(s) 429
BssSI CACGAG 2 cut(s) 491, 605
Bst1107I GTATAC 1 cut(s) 429
Bst2BI CACGAG 2 cut(s) 491, 605
Bst2UI CCWGG 1 cut(s) 562
Bst4CI ACNGT 2 cut(s) 194, 724
Bst6I CTCTTC 1 cut(s) 353
BstAFI CTTAAG 1 cut(s) 704
BstAPI GCANNNNNTGC 1 cut(s) 42
BstBI TTCGAA 1 cut(s) 95
BstC8I GCNNGC 8 cut(s) 9, 245, 449, 576, 578, 594, 661, 795
BstDEI CTNAG 3 cut(s) 548, 746, 782
BstDSI CCRYGG 2 cut(s) 664, 723
BstENI CCTNNNNNAGG 1 cut(s) 439
BstF5I GGATG 3 cut(s) 543, 640, 836
BstFNI CGCG 3 cut(s) 576, 578, 689
BstHHI GCGC 3 cut(s) 576, 578, 580
BstKTI GATC 4 cut(s) 300, 459, 675, 696
BstMAI GTCTC 2 cut(s) 342, 713
BstMBI GATC 4 cut(s) 297, 456, 672, 693
BstMCI CGRYCG 1 cut(s) 300
BstMWI GCNNNNNNNGC 4 cut(s) 42, 51, 253, 686
BstNI CCWGG 1 cut(s) 562
BstPAI GACNNNNGTC 1 cut(s) 563
BstSCI CCNGG 3 cut(s) 461, 542, 560
BstSFI CTRYAG 1 cut(s) 527
BstUI CGCG 3 cut(s) 576, 578, 689
BstV1I GCAGC 1 cut(s) 513
BstZ17I GTATAC 1 cut(s) 429
BsuI GTATCC 1 cut(s) 31
BsuRI GGCC 5 cut(s) 287, 308, 542, 659, 663
BtgI CCRYGG 2 cut(s) 664, 723
BtrI CACGTC 1 cut(s) 423
BtsCI GGATG 3 cut(s) 543, 640, 836
BtsI GCAGTG 1 cut(s) 804
BtsIMutI CAGTG 1 cut(s) 804
BveI ACCTGC 1 cut(s) 520
Cac8I GCNNGC 8 cut(s) 9, 245, 449, 576, 578, 594, 661, 795
CfoI GCGC 3 cut(s) 576, 578, 580
Cfr10I RCCGGY 1 cut(s) 659
Cfr13I GGNCC 5 cut(s) 250, 286, 307, 541, 657
CseI GACGC 2 cut(s) 201, 695
Csp6I GTAC 2 cut(s) 466, 649
CviAII CATG 1 cut(s) 631
CviQI GTAC 2 cut(s) 466, 649
DdeI CTNAG 3 cut(s) 548, 746, 782
DpnI GATC 4 cut(s) 299, 458, 674, 695
DpnII GATC 4 cut(s) 297, 456, 672, 693
EaeI YGGCCR 1 cut(s) 661
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
EciI GGCGGA 1 cut(s) 695
Ecl136II GAGCTC 2 cut(s) 277, 786
Eco24I GRGCYC 2 cut(s) 279, 788
Eco31I GGTCTC 1 cut(s) 713
Eco47I GGWCC 1 cut(s) 250
Eco53kI GAGCTC 2 cut(s) 277, 786
Eco88I CYCGRG 3 cut(s) 309, 339, 787
EcoICRI GAGCTC 2 cut(s) 277, 786
EcoNI CCTNNNNNAGG 1 cut(s) 439
EcoRII CCWGG 1 cut(s) 560
EcoT38I GRGCYC 2 cut(s) 279, 788
FaeI CATG 1 cut(s) 634
FaiI YATR 4 cut(s) 185, 429, 632, 828
FatI CATG 1 cut(s) 630
FbaI TGATCA 1 cut(s) 693
FblI GTMKAC 1 cut(s) 428
Fnu4HI GCNGC 3 cut(s) 205, 527, 618
FokI GGATG 2 cut(s) 550, 647
FriOI GRGCYC 2 cut(s) 279, 788
FseI GGCCGGCC 1 cut(s) 663
Fsp4HI GCNGC 3 cut(s) 205, 527, 618
GlaI GCGC 3 cut(s) 575, 577, 579
GluI GCNGC 3 cut(s) 205, 527, 618
HaeIII GGCC 5 cut(s) 287, 308, 542, 659, 663
HapII CCGG 4 cut(s) 462, 487, 544, 660
HgaI GACGC 2 cut(s) 201, 695
HhaI GCGC 3 cut(s) 576, 578, 580
Hin1II CATG 1 cut(s) 634
Hin6I GCGC 3 cut(s) 574, 576, 578
HinP1I GCGC 3 cut(s) 574, 576, 578
HindIII AAGCTT 2 cut(s) 5, 160
HinfI GANTC 3 cut(s) 97, 500, 601
HpaII CCGG 4 cut(s) 462, 487, 544, 660
HphI GGTGA 2 cut(s) 652, 703
Hpy166II GTNNAC 1 cut(s) 429
Hpy188I TCNGA 2 cut(s) 326, 511
Hpy188III TCNNGA 4 cut(s) 14, 493, 535, 605
Hpy8I GTNNAC 1 cut(s) 429
Hpy99I CGWCG 6 cut(s) 305, 374, 424, 689, 757, 766
HpyCH4III ACNGT 2 cut(s) 194, 724
HpyCH4IV ACGT 1 cut(s) 422
HpyCH4V TGCA 2 cut(s) 382, 529
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 51, 253, 686
HpyF3I CTNAG 3 cut(s) 548, 746, 782
HpySE526I ACGT 1 cut(s) 422
Hsp92II CATG 1 cut(s) 634
HspAI GCGC 3 cut(s) 574, 576, 578
KroI GCCGGC 1 cut(s) 659
KroNI GCCGGC 1 cut(s) 661
Ksp22I TGATCA 1 cut(s) 693
Kzo9I GATC 4 cut(s) 297, 456, 672, 693
LmnI GCTCC 2 cut(s) 444, 571
Lsp1109I GCAGC 1 cut(s) 513
LweI GCATC 1 cut(s) 625
MaeII ACGT 1 cut(s) 422
MaeIII GTNAC 1 cut(s) 493
MalI GATC 4 cut(s) 299, 458, 674, 695
MboI GATC 4 cut(s) 297, 456, 672, 693
MboII GAAGA 1 cut(s) 340
MhlI GDGCHC 2 cut(s) 279, 788
MluCI AATT 3 cut(s) 377, 803, 817
MlyI GAGTC 1 cut(s) 509
MroNI GCCGGC 1 cut(s) 659
MseI TTAA 3 cut(s) 113, 147, 705
MspA1I CMGCKG 2 cut(s) 526, 625
MspCI CTTAAG 1 cut(s) 704
MspI CCGG 4 cut(s) 462, 487, 544, 660
MspR9I CCNGG 3 cut(s) 463, 544, 562
Mva1269I GAATGC 2 cut(s) 230, 739
MvaI CCWGG 1 cut(s) 562
MvnI CGCG 3 cut(s) 576, 578, 689
MwoI GCNNNNNNNGC 4 cut(s) 42, 51, 253, 686
NaeI GCCGGC 1 cut(s) 661
NciI CCSGG 2 cut(s) 463, 544
NdeII GATC 4 cut(s) 297, 456, 672, 693
NgoMIV GCCGGC 1 cut(s) 659
NlaIII CATG 1 cut(s) 634
NlaIV GGNNCC 2 cut(s) 446, 621
NmuCI GTSAC 1 cut(s) 493
NspV TTCGAA 1 cut(s) 95
PauI GCGCGC 2 cut(s) 574, 576
PcsI WCGNNNNNNNCGW 4 cut(s) 672, 681, 758, 761
PctI GAATGC 2 cut(s) 230, 739
PdiI GCCGGC 1 cut(s) 661
PfeI GAWTC 2 cut(s) 97, 601
PflFI GACNNNGTC 1 cut(s) 499
PkrI GCNGC 3 cut(s) 206, 528, 619
Ple19I CGATCG 1 cut(s) 300
PleI GAGTC 1 cut(s) 508
PpsI GAGTC 1 cut(s) 508
PshAI GACNNNNGTC 1 cut(s) 563
Psp124BI GAGCTC 2 cut(s) 279, 788
Psp6I CCWGG 1 cut(s) 560
PspGI CCWGG 1 cut(s) 560
PspN4I GGNNCC 2 cut(s) 446, 621
PspPI GGNCC 5 cut(s) 250, 286, 307, 541, 657
PstI CTGCAG 1 cut(s) 531
PsyI GACNNNGTC 1 cut(s) 499
PteI GCGCGC 2 cut(s) 574, 576
PvuI CGATCG 1 cut(s) 300
PvuII CAGCTG 1 cut(s) 526
RigI GGCCGGCC 1 cut(s) 663
RsaI GTAC 2 cut(s) 467, 650
RsaNI GTAC 2 cut(s) 466, 649
SacI GAGCTC 2 cut(s) 279, 788
SaqAI TTAA 3 cut(s) 113, 147, 705
SatI GCNGC 3 cut(s) 205, 527, 618
Sau3AI GATC 4 cut(s) 297, 456, 672, 693
Sau96I GGNCC 5 cut(s) 250, 286, 307, 541, 657
SchI GAGTC 1 cut(s) 509
ScrFI CCNGG 3 cut(s) 463, 544, 562
SduI GDGCHC 2 cut(s) 279, 788
SfaNI GCATC 1 cut(s) 625
SfcI CTRYAG 1 cut(s) 527
SfuI TTCGAA 1 cut(s) 95
SinI GGWCC 1 cut(s) 250
SmlI CTYRAG 1 cut(s) 704
SmoI CTYRAG 1 cut(s) 704
Sse9I AATT 3 cut(s) 377, 803, 817
SsiI CCGC 7 cut(s) 204, 617, 623, 638, 646, 680, 689
SstI GAGCTC 2 cut(s) 279, 788
StyD4I CCNGG 3 cut(s) 461, 542, 560
TaaI ACNGT 2 cut(s) 194, 724
TaiI ACGT 1 cut(s) 425
TaqI TCGA 6 cut(s) 15, 95, 225, 270, 300, 567
TaqII GACCGA 1 cut(s) 512
TasI AATT 3 cut(s) 377, 803, 817
TauI GCSGC 2 cut(s) 207, 620
TfiI GAWTC 2 cut(s) 97, 601
Tru1I TTAA 3 cut(s) 113, 147, 705
Tru9I TTAA 3 cut(s) 113, 147, 705
TscAI CASTG 1 cut(s) 804
TseFI GTSAC 1 cut(s) 493
TseI GCWGC 1 cut(s) 526
Tsp45I GTSAC 1 cut(s) 493
TspDTI ATGAA 2 cut(s) 17, 619
TspRI CASTG 1 cut(s) 804
Tth111I GACNNNGTC 1 cut(s) 499
Vha464I CTTAAG 1 cut(s) 704
VpaK11BI GGWCC 1 cut(s) 250
XagI CCTNNNNNAGG 1 cut(s) 439
XapI RAATTY 1 cut(s) 803
XmiI GTMKAC 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.