pycom17g00990

Belongs to the universal ribosomal protein uL22 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001814
Physical Location & Seq
Forward (+)
710348 .. 712197
1850 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 558 bp
ATGGTGAAGTACTCGAGGGAGCCCAATAATCCCACCAAGTCCTGCAAGGCCCAGGGTGAGGGTCTAAGAGTTCATTTCAAGAACACGAGAGAGACTGCCCATGCCATCAGGAAGATGCATTTGGCAAAGGCCAAAAGGTACTTGGAAGATGTTTTAGCCCACAAGCAAGCCATTCCCTTCCGCCGCTTTTGCCGCGGTGTTGGCCGAACTGCGCAGGCGAAGAACCGTCAGTCCAATGGTCAGGGGCGTTGGCCCGTTAAGTCTGCTAATTTCATTCTTGATTTGCTCAAGAATGCTGAGAGCAATGCTGAGGTAAATGTCAAGGGGCTTGATGTGGATTCGCTGTACGTCTCTCACATCCAGGTCAACCAGGCTCAGAAACAGAGACGCCGCACATATCGTGCTCATGGGAGAATAAATCCTTATATGTCATCTCCCTGCCATATCGAGTTGATTTTGTCAGAGAAAGAAGAGGCAGTAAAGAAAGAGCCTGATACTCAGTTGGCTCCCAGGAAGTCGAGGAAAGGTCAAGCACTGCAGAGCGGTGCTTCCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L22

Protein Analysis

186

Amino Acids

20.98

Weight (kDa)

10.34

Isoelectric Point (pI)

61.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L22 PF00237 17 - 154 8.8e-33 Ribosomal protein L22p/L17e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 213
AccBSI CCGCTC 1 cut(s) 543
AccII CGCG 1 cut(s) 195
AciI CCGC 6 cut(s) 181, 184, 193, 195, 391, 543
AcoI YGGCCR 1 cut(s) 202
AcyI GRCGYC 1 cut(s) 388
AfaI GTAC 3 cut(s) 11, 140, 347
AfiI CCNNNNNNNGG 1 cut(s) 58
AgsI TTSAA 1 cut(s) 79
AjnI CCWGG 4 cut(s) 51, 360, 369, 509
AjuI GAANNNNNNNTTGG 2 cut(s) 104, 136
AloI GAACNNNNNNTCC 2 cut(s) 215, 247
Alw21I GWGCWC 1 cut(s) 406
Alw26I GTCTC 3 cut(s) 86, 355, 379
Ama87I CYCGRG 1 cut(s) 13
AoxI GGCC 4 cut(s) 48, 129, 202, 251
AspLEI GCGC 1 cut(s) 214
AspS9I GGNCC 2 cut(s) 49, 252
AsuHPI GGTGA 2 cut(s) 16, 68
AvaI CYCGRG 1 cut(s) 13
BanII GRGCYC 1 cut(s) 24
BauI CACGAG 1 cut(s) 85
Bbv12I GWGCWC 1 cut(s) 406
BbvCI CCTCAGC 1 cut(s) 309
BccI CCATC 1 cut(s) 113
BciT130I CCWGG 4 cut(s) 53, 362, 371, 511
BcoDI GTCTC 3 cut(s) 86, 355, 379
BfmI CTRYAG 1 cut(s) 536
BisI GCNGC 3 cut(s) 184, 193, 391
BlsI GCNGC 3 cut(s) 185, 194, 392
BmcAI AGTACT 1 cut(s) 11
Bme1390I CCNGG 4 cut(s) 53, 362, 371, 511
BmeT110I CYCGRG 1 cut(s) 13
BmgT120I GGNCC 2 cut(s) 49, 252
BmiI GGNNCC 2 cut(s) 21, 507
BmrFI CCNGG 4 cut(s) 53, 362, 371, 511
BmsI GCATC 1 cut(s) 105
Bpu10I CCTNAGC 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 272
BsaHI GRCGYC 1 cut(s) 388
BsaJI CCNNGG 4 cut(s) 51, 52, 193, 509
Bsc4I CCNNNNNNNGG 1 cut(s) 58
Bse3DI GCAATG 1 cut(s) 310
BseBI CCWGG 4 cut(s) 53, 362, 371, 511
BseDI CCNNGG 4 cut(s) 51, 52, 193, 509
BseGI GGATG 1 cut(s) 357
BseLI CCNNNNNNNGG 1 cut(s) 58
BseMI GCAATG 1 cut(s) 310
BseMII CTCAG 4 cut(s) 288, 300, 389, 512
Bsh1236I CGCG 1 cut(s) 195
BshFI GGCC 4 cut(s) 50, 131, 204, 253
BsiHKAI GWGCWC 1 cut(s) 406
BsiHKCI CYCGRG 1 cut(s) 13
BslI CCNNNNNNNGG 1 cut(s) 58
BsmAI GTCTC 3 cut(s) 86, 355, 379
BsmBI CGTCTC 2 cut(s) 355, 379
BsmI GAATGC 1 cut(s) 298
BsnI GGCC 4 cut(s) 50, 131, 204, 253
BsoBI CYCGRG 1 cut(s) 13
Bsp1286I GDGCHC 2 cut(s) 24, 406
BspACI CCGC 6 cut(s) 181, 184, 193, 195, 391, 543
BspANI GGCC 4 cut(s) 50, 131, 204, 253
BspCNI CTCAG 4 cut(s) 289, 301, 388, 511
BspFNI CGCG 1 cut(s) 195
BspLI GGNNCC 2 cut(s) 21, 507
BspMAI CTGCAG 1 cut(s) 540
BsrBI CCGCTC 1 cut(s) 543
BsrDI GCAATG 1 cut(s) 310
BssECI CCNNGG 4 cut(s) 51, 52, 193, 509
BssNI GRCGYC 1 cut(s) 388
BssSI CACGAG 1 cut(s) 85
Bst2BI CACGAG 1 cut(s) 85
Bst2UI CCWGG 4 cut(s) 53, 362, 371, 511
Bst4CI ACNGT 1 cut(s) 227
Bst6I CTCTTC 1 cut(s) 465
BstACI GRCGYC 1 cut(s) 388
BstC8I GCNNGC 2 cut(s) 168, 216
BstDEI CTNAG 5 cut(s) 65, 297, 309, 375, 498
BstDSI CCRYGG 1 cut(s) 193
BstF5I GGATG 1 cut(s) 357
BstFNI CGCG 1 cut(s) 195
BstHHI GCGC 1 cut(s) 214
BstMAI GTCTC 3 cut(s) 86, 355, 379
BstMWI GCNNNNNNNGC 3 cut(s) 189, 192, 201
BstNI CCWGG 4 cut(s) 53, 362, 371, 511
BstSCI CCNGG 4 cut(s) 51, 360, 369, 509
BstSFI CTRYAG 1 cut(s) 536
BstUI CGCG 1 cut(s) 195
BsuRI GGCC 4 cut(s) 50, 131, 204, 253
BtgI CCRYGG 1 cut(s) 193
BtsCI GGATG 1 cut(s) 357
BtsI GCAGTG 1 cut(s) 533
BtsIMutI CAGTG 1 cut(s) 533
Cac8I GCNNGC 2 cut(s) 168, 216
CfoI GCGC 1 cut(s) 214
Cfr13I GGNCC 2 cut(s) 49, 252
Cfr42I CCGCGG 1 cut(s) 196
CseI GACGC 1 cut(s) 396
Csp6I GTAC 3 cut(s) 10, 139, 346
CviAII CATG 2 cut(s) 101, 407
CviQI GTAC 3 cut(s) 10, 139, 346
DdeI CTNAG 5 cut(s) 65, 297, 309, 375, 498
EaeI YGGCCR 1 cut(s) 202
Eam1104I CTCTTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 465
EciI GGCGGA 1 cut(s) 170
Eco24I GRGCYC 1 cut(s) 24
Eco88I CYCGRG 1 cut(s) 13
EcoRII CCWGG 4 cut(s) 51, 360, 369, 509
EcoT22I ATGCAT 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 24
Esp3I CGTCTC 2 cut(s) 355, 379
FaeI CATG 2 cut(s) 104, 410
FaiI YATR 6 cut(s) 102, 397, 408, 426, 428, 444
FatI CATG 2 cut(s) 100, 406
Fnu4HI GCNGC 3 cut(s) 184, 193, 391
FokI GGATG 1 cut(s) 344
FriOI GRGCYC 1 cut(s) 24
Fsp4HI GCNGC 3 cut(s) 184, 193, 391
FspI TGCGCA 1 cut(s) 213
GlaI GCGC 1 cut(s) 213
GluI GCNGC 3 cut(s) 184, 193, 391
HaeIII GGCC 4 cut(s) 50, 131, 204, 253
HgaI GACGC 1 cut(s) 396
HhaI GCGC 1 cut(s) 214
Hin1I GRCGYC 1 cut(s) 388
Hin1II CATG 2 cut(s) 104, 410
Hin6I GCGC 1 cut(s) 212
HinP1I GCGC 1 cut(s) 212
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HinfI GANTC 1 cut(s) 338
HphI GGTGA 2 cut(s) 16, 68
Hpy166II GTNNAC 1 cut(s) 367
Hpy188I TCNGA 2 cut(s) 378, 463
Hpy188III TCNNGA 5 cut(s) 79, 109, 278, 289, 555
Hpy8I GTNNAC 1 cut(s) 367
HpyAV CCTTC 1 cut(s) 187
HpyCH4III ACNGT 1 cut(s) 227
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 3 cut(s) 45, 118, 538
HpyF10VI GCNNNNNNNGC 3 cut(s) 189, 192, 201
HpyF3I CTNAG 5 cut(s) 65, 297, 309, 375, 498
HpySE526I ACGT 1 cut(s) 348
Hsp92I GRCGYC 1 cut(s) 388
Hsp92II CATG 2 cut(s) 104, 410
HspAI GCGC 1 cut(s) 212
KspI CCGCGG 1 cut(s) 196
LmnI GCTCC 2 cut(s) 19, 511
LweI GCATC 1 cut(s) 105
MaeII ACGT 1 cut(s) 348
MbiI CCGCTC 1 cut(s) 543
MboII GAAGA 4 cut(s) 124, 158, 232, 482
MhlI GDGCHC 2 cut(s) 24, 406
MluCI AATT 1 cut(s) 268
MnlI CCTC 5 cut(s) 9, 52, 304, 466, 513
Mph1103I ATGCAT 1 cut(s) 120
MseI TTAA 1 cut(s) 258
MspA1I CMGCKG 1 cut(s) 195
MspR9I CCNGG 4 cut(s) 53, 362, 371, 511
Mva1269I GAATGC 1 cut(s) 298
MvaI CCWGG 4 cut(s) 53, 362, 371, 511
MvnI CGCG 1 cut(s) 195
MwoI GCNNNNNNNGC 3 cut(s) 189, 192, 201
NlaIII CATG 2 cut(s) 104, 410
NlaIV GGNNCC 2 cut(s) 21, 507
NsbI TGCGCA 1 cut(s) 213
NsiI ATGCAT 1 cut(s) 120
PaeR7I CTCGAG 1 cut(s) 13
PasI CCCWGGG 1 cut(s) 52
PctI GAATGC 1 cut(s) 298
PfeI GAWTC 1 cut(s) 338
PkrI GCNGC 3 cut(s) 185, 194, 392
Psp6I CCWGG 4 cut(s) 51, 360, 369, 509
PspGI CCWGG 4 cut(s) 51, 360, 369, 509
PspN4I GGNNCC 2 cut(s) 21, 507
PspPI GGNCC 2 cut(s) 49, 252
PspXI VCTCGAGB 1 cut(s) 13
PstI CTGCAG 1 cut(s) 540
RsaI GTAC 3 cut(s) 11, 140, 347
RsaNI GTAC 3 cut(s) 10, 139, 346
SacII CCGCGG 1 cut(s) 196
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 3 cut(s) 184, 193, 391
Sau96I GGNCC 2 cut(s) 49, 252
ScaI AGTACT 1 cut(s) 11
ScrFI CCNGG 4 cut(s) 53, 362, 371, 511
SduI GDGCHC 2 cut(s) 24, 406
SetI ASST 5 cut(s) 140, 315, 351, 366, 529
SfaNI GCATC 1 cut(s) 105
SfcI CTRYAG 1 cut(s) 536
Sfr274I CTCGAG 1 cut(s) 13
Sfr303I CCGCGG 1 cut(s) 196
SgrBI CCGCGG 1 cut(s) 196
SlaI CTCGAG 1 cut(s) 13
SmlI CTYRAG 2 cut(s) 13, 287
SmoI CTYRAG 2 cut(s) 13, 287
Sse9I AATT 1 cut(s) 268
SsiI CCGC 6 cut(s) 181, 184, 193, 195, 391, 543
StyD4I CCNGG 4 cut(s) 51, 360, 369, 509
TaaI ACNGT 1 cut(s) 227
TaiI ACGT 1 cut(s) 351
TaqI TCGA 3 cut(s) 14, 447, 518
TasI AATT 1 cut(s) 268
TatI WGTACW 1 cut(s) 9
TauI GCSGC 3 cut(s) 186, 195, 393
TfiI GAWTC 1 cut(s) 338
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TscAI CASTG 1 cut(s) 540
TspDTI ATGAA 2 cut(s) 62, 262
TspRI CASTG 1 cut(s) 540
XcmI CCANNNNNNNNNTGG 1 cut(s) 139
XhoI CTCGAG 1 cut(s) 13
ZrmI AGTACT 1 cut(s) 11
Zsp2I ATGCAT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.