pycom17g05080

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002017
Physical Location & Seq
Reverse (-)
3528160 .. 3530191
2032 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g05080.2

Sequence Viewer

Length: 1299 bp
ATGCCGTACTACACACACGCCGAGGATGAAGTCACCGACTTCGACGAGTACGATCCGACTCCGTACGGTGGCGGATACGACATATACCTGACCTATGGCCGCCCTCTCGACCCGTCCGATGAGTCCTGCCACCCTAACTCATCGCCGTCCGATGATTTCGATTACGATCGCCCCCAGTTCTCATCCTCCTCCGAGCCCGCCGCCTACGCTGACGAGGCTCTGGAAGACGAGTACACTCACTACGCCCGACCCACGCACCGACCCGGACCACCTATCGGGTTCAATCCGGGCGGGGGACATCCAGAGGGAGAGTACGAAGGCAGGCCCCAGCCGGCCTATGGGTTCCAGCCTGGCGGGGAGGAGAGGCCCGAATCGGGTTATGGTCGGAAGCCGGAGTATGGACAATCCGGTTCGGAATACGGGTCTGGGTATCAACGAAGGCCCGAATCCGATGAGCCCGTCTCCGAATATGGATCTGGGTATGGTCGGAAGCCGGTGTACGAGGCGCCCGAATCGGAGTATGGATCTGGATACGGTCGACAGCCCGAGTATAAGGCACCGGAACCAGAGTATGGATCTGGGTACGGGAGAAAGCCTGAGTACGAGGCACCGGAGTCCGAATATGGATCTGGGTACGGTCGAAAGCCCGAGTATAAGGCATCGGAACCCGAGTATGGATCTGGGTACAGGAGAAAACCCGAGTACGAGGCACCCGAGTCCGAATATGGATCTGGGTACGGGAGAAAGCCCGAGTTTGAAGCACTGCCAGCTGAATTCGGATCTGGGTATGATCGGAAGCCGAGCTACGGTGAGGAATCTGGTTATGGCGATGAGCCGCCGAGAAAGCCGAGCTATGGTGAGGAAGGCGGTTATGGAGAAGAGCCACCGAGAAGGCCAAGCTATGGGCGGCCCAGTTATGAGACGCCAGAAAGCGAGGAGAGGCCGAGCTATGGGAGGACTGAGTTTGAGAGGCCGGGTTACGGCGAAGAGCCGCCGAGGAGGACCGGCTATGGAGATGAGCCGCCACCCCCGAGGCCAAGCTATGGGAGGCCTGGCTCCGAGGGCCAGGAAAGTGGGGAGTATGAGAAGCCCAGTTATGGGAGGAGCGAGGAGCAGGAGTACCGCCGCCCTGGTGGTTATGAGAGGCGCGGCGACGATGATTCTGATTCCGAGCGTCCTAAGTACGGTGAAGAAGGCTATGGTCGCAAGAAATACGGAAATGACGACTCAGATGACGATGAGGAGAAAAAGCAGCGCCGCCACAAGCACCACCATCGCAAGAGCTATGACGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

433

Amino Acids

48.91

Weight (kDa)

4.66

Isoelectric Point (pI)

69.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 505, 556, 607, 709
AccB7I CCANNNNNTGG 3 cut(s) 572, 902, 1043
AccI GTMKAC 1 cut(s) 538
AccII CGCG 1 cut(s) 1149
AclWI GGATC 8 cut(s) 47, 481, 532, 583, 634, 685, 736, 787
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 1 cut(s) 773
AcyI GRCGYC 2 cut(s) 506, 923
AgsI TTSAA 2 cut(s) 283, 758
AjnI CCWGG 4 cut(s) 349, 1051, 1065, 1129
AluBI AGCT 7 cut(s) 770, 804, 852, 900, 948, 1041, 1284
AluI AGCT 7 cut(s) 770, 804, 852, 900, 948, 1041, 1284
Alw26I GTCTC 2 cut(s) 466, 914
AlwI GGATC 8 cut(s) 47, 481, 532, 583, 634, 685, 736, 787
Ama87I CYCGRG 7 cut(s) 545, 647, 668, 698, 713, 749, 1030
ApeKI GCWGC 1 cut(s) 1252
ApoI RAATTY 1 cut(s) 773
AspLEI GCGC 3 cut(s) 508, 1149, 1257
AspS9I GGNCC 7 cut(s) 266, 324, 366, 441, 909, 1002, 1063
AsuC2I CCSGG 3 cut(s) 264, 288, 975
AsuHPI GGTGA 4 cut(s) 25, 821, 869, 1199
AvaI CYCGRG 7 cut(s) 545, 647, 668, 698, 713, 749, 1030
AvaII GGWCC 2 cut(s) 266, 1002
BanI GGYRCC 4 cut(s) 505, 556, 607, 709
BanII GRGCYC 2 cut(s) 198, 459
BarI GAAGNNNNNNTAC 4 cut(s) 482, 514, 788, 820
BbsI GAAGAC 1 cut(s) 231
BbvI GCAGC 1 cut(s) 1264
BccI CCATC 1 cut(s) 1281
BceAI ACGGC 2 cut(s) 130, 997
BcgI CGANNNNNNTGC 2 cut(s) 1256, 1290
BciT130I CCWGG 4 cut(s) 351, 1053, 1067, 1131
BciVI GTATCC 2 cut(s) 68, 524
BcnI CCSGG 3 cut(s) 264, 288, 975
BcoDI GTCTC 2 cut(s) 466, 914
BfoI RGCGCY 2 cut(s) 509, 1258
BfuI GTATCC 2 cut(s) 68, 524
Bme1390I CCNGG 7 cut(s) 264, 288, 351, 975, 1053, 1067, 1131
Bme18I GGWCC 2 cut(s) 266, 1002
BmeT110I CYCGRG 7 cut(s) 545, 647, 668, 698, 713, 749, 1030
BmgT120I GGNCC 7 cut(s) 266, 324, 366, 441, 909, 1002, 1063
BmiI GGNNCC 9 cut(s) 326, 344, 507, 558, 564, 609, 666, 711, 1057
BmrFI CCNGG 7 cut(s) 264, 288, 351, 975, 1053, 1067, 1131
BmrI ACTGGG 3 cut(s) 169, 906, 1086
BmsI GCATC 1 cut(s) 668
BmuI ACTGGG 3 cut(s) 169, 906, 1086
BpiI GAAGAC 1 cut(s) 231
BplI GAGNNNNNCTC 2 cut(s) 446, 478
BpuMI CCSGG 3 cut(s) 264, 288, 975
BsaBI GATNNNNATC 1 cut(s) 165
BsaHI GRCGYC 2 cut(s) 506, 923
BsaJI CCNNGG 5 cut(s) 21, 995, 1031, 1059, 1129
BsaWI WCCGGW 3 cut(s) 407, 559, 610
BsaXI ACNNNNNCTCC 2 cut(s) 1103, 1133
Bse118I RCCGGY 3 cut(s) 331, 493, 1004
Bse1I ACTGG 3 cut(s) 175, 912, 1092
Bse8I GATNNNNATC 1 cut(s) 165
BseBI CCWGG 4 cut(s) 351, 1053, 1067, 1131
BseDI CCNNGG 5 cut(s) 21, 995, 1031, 1059, 1129
BseGI GGATG 3 cut(s) 31, 182, 298
BseJI GATNNNNATC 1 cut(s) 165
BseMII CTCAG 3 cut(s) 588, 951, 1242
BseNI ACTGG 3 cut(s) 175, 912, 1092
BseRI GAGGAG 7 cut(s) 178, 374, 950, 1012, 1117, 1124, 1256
BseXI GCAGC 1 cut(s) 1264
BseYI CCCAGC 1 cut(s) 327
Bsh1236I CGCG 1 cut(s) 1149
Bsh1285I CGRYCG 3 cut(s) 169, 538, 640
BshNI GGYRCC 4 cut(s) 505, 556, 607, 709
BsiEI CGRYCG 3 cut(s) 169, 538, 640
BsiHKCI CYCGRG 7 cut(s) 545, 647, 668, 698, 713, 749, 1030
BsiWI CGTACG 1 cut(s) 63
BslFI GGGAC 1 cut(s) 309
BsmAI GTCTC 2 cut(s) 466, 914
BsmBI CGTCTC 2 cut(s) 466, 914
BsmFI GGGAC 1 cut(s) 309
BsoBI CYCGRG 7 cut(s) 545, 647, 668, 698, 713, 749, 1030
Bsp1286I GDGCHC 2 cut(s) 198, 459
BspCNI CTCAG 3 cut(s) 589, 952, 1241
BspFNI CGCG 1 cut(s) 1149
BspLI GGNNCC 9 cut(s) 326, 344, 507, 558, 564, 609, 666, 711, 1057
BspPI GGATC 8 cut(s) 47, 481, 532, 583, 634, 685, 736, 787
BspQI GCTCTTC 2 cut(s) 873, 981
BspT107I GGYRCC 4 cut(s) 505, 556, 607, 709
BsrFI RCCGGY 3 cut(s) 331, 493, 1004
BsrI ACTGG 3 cut(s) 175, 912, 1092
BssAI RCCGGY 3 cut(s) 331, 493, 1004
BssECI CCNNGG 5 cut(s) 21, 995, 1031, 1059, 1129
BssNI GRCGYC 2 cut(s) 506, 923
Bst2UI CCWGG 4 cut(s) 351, 1053, 1067, 1131
Bst4CI ACNGT 5 cut(s) 68, 536, 638, 809, 1187
Bst6I CTCTTC 2 cut(s) 873, 981
BstACI GRCGYC 2 cut(s) 506, 923
BstC8I GCNNGC 4 cut(s) 198, 323, 333, 768
BstDEI CTNAG 4 cut(s) 597, 960, 1179, 1228
BstF5I GGATG 3 cut(s) 31, 182, 298
BstFNI CGCG 1 cut(s) 1149
BstH2I RGCGCY 2 cut(s) 509, 1258
BstHHI GCGC 3 cut(s) 508, 1149, 1257
BstMAI GTCTC 2 cut(s) 466, 914
BstMCI CGRYCG 3 cut(s) 169, 538, 640
BstMWI GCNNNNNNNGC 6 cut(s) 206, 215, 767, 844, 1062, 1203
BstNI CCWGG 4 cut(s) 351, 1053, 1067, 1131
BstSCI CCNGG 7 cut(s) 262, 286, 349, 973, 1051, 1065, 1129
BstUI CGCG 1 cut(s) 1149
BstV1I GCAGC 1 cut(s) 1264
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 7 cut(s) 473, 524, 575, 626, 677, 728, 779
BstXI CCANNNNNNTGG 1 cut(s) 1073
BstYI RGATCY 7 cut(s) 473, 524, 575, 626, 677, 728, 779
BsuI GTATCC 2 cut(s) 68, 524
BtgZI GCGATG 3 cut(s) 126, 843, 1259
BtsCI GGATG 3 cut(s) 31, 182, 298
BtsI GCAGTG 1 cut(s) 761
BtsIMutI CAGTG 1 cut(s) 761
Cac8I GCNNGC 4 cut(s) 198, 323, 333, 768
CfoI GCGC 3 cut(s) 508, 1149, 1257
Cfr10I RCCGGY 3 cut(s) 331, 493, 1004
Cfr13I GGNCC 7 cut(s) 266, 324, 366, 441, 909, 1002, 1063
CseI GACGC 2 cut(s) 931, 1163
DdeI CTNAG 4 cut(s) 597, 960, 1179, 1228
DinI GGCGCC 1 cut(s) 507
EaeI YGGCCR 1 cut(s) 97
Eam1104I CTCTTC 2 cut(s) 873, 981
EarI CTCTTC 2 cut(s) 873, 981
EciI GGCGGA 1 cut(s) 87
Eco147I AGGCCT 1 cut(s) 1051
Eco24I GRGCYC 2 cut(s) 198, 459
Eco47I GGWCC 2 cut(s) 266, 1002
Eco88I CYCGRG 7 cut(s) 545, 647, 668, 698, 713, 749, 1030
EcoO109I RGGNCCY 1 cut(s) 324
EcoRI GAATTC 1 cut(s) 773
EcoRII CCWGG 4 cut(s) 349, 1051, 1065, 1129
EcoT38I GRGCYC 2 cut(s) 198, 459
EgeI GGCGCC 1 cut(s) 507
EheI GGCGCC 1 cut(s) 507
Esp3I CGTCTC 2 cut(s) 466, 914
FaqI GGGAC 1 cut(s) 309
FauI CCCGC 3 cut(s) 205, 284, 347
FblI GTMKAC 1 cut(s) 538
FokI GGATG 3 cut(s) 38, 169, 285
FriOI GRGCYC 2 cut(s) 198, 459
GlaI GCGC 3 cut(s) 507, 1148, 1256
GsaI CCCAGC 1 cut(s) 331
HaeII RGCGCY 2 cut(s) 509, 1258
HgaI GACGC 2 cut(s) 931, 1163
HhaI GCGC 3 cut(s) 508, 1149, 1257
Hin1I GRCGYC 2 cut(s) 506, 923
Hin6I GCGC 3 cut(s) 506, 1147, 1255
HinP1I GCGC 3 cut(s) 506, 1147, 1255
HincII GTYRAC 1 cut(s) 539
HindII GTYRAC 1 cut(s) 539
HphI GGTGA 4 cut(s) 25, 821, 869, 1199
Hpy166II GTNNAC 3 cut(s) 234, 499, 539
Hpy188III TCNNGA 4 cut(s) 107, 221, 302, 528
Hpy8I GTNNAC 3 cut(s) 234, 499, 539
Hpy99I CGWCG 2 cut(s) 47, 1157
HpyAV CCTTC 5 cut(s) 311, 432, 857, 885, 1187
HpyCH4III ACNGT 5 cut(s) 68, 536, 638, 809, 1187
HpyF10VI GCNNNNNNNGC 6 cut(s) 206, 215, 767, 844, 1062, 1203
HpyF3I CTNAG 4 cut(s) 597, 960, 1179, 1228
Hsp92I GRCGYC 2 cut(s) 506, 923
HspAI GCGC 3 cut(s) 506, 1147, 1255
KasI GGCGCC 1 cut(s) 505
KroI GCCGGC 1 cut(s) 331
KroNI GCCGGC 1 cut(s) 333
LguI GCTCTTC 2 cut(s) 873, 981
LmnI GCTCC 3 cut(s) 1061, 1104, 1111
Lsp1109I GCAGC 1 cut(s) 1264
LweI GCATC 1 cut(s) 668
MaeIII GTNAC 2 cut(s) 31, 977
MboII GAAGA 4 cut(s) 236, 890, 998, 1202
MflI RGATCY 7 cut(s) 473, 524, 575, 626, 677, 728, 779
MhlI GDGCHC 2 cut(s) 198, 459
MluCI AATT 1 cut(s) 773
Mly113I GGCGCC 1 cut(s) 506
MlyI GAGTC 5 cut(s) 52, 131, 623, 725, 1220
MmeI TCCRAC 3 cut(s) 80, 365, 467
MroNI GCCGGC 1 cut(s) 331
MspA1I CMGCKG 1 cut(s) 770
MspR9I CCNGG 7 cut(s) 264, 288, 351, 975, 1053, 1067, 1131
MvaI CCWGG 4 cut(s) 351, 1053, 1067, 1131
MvnI CGCG 1 cut(s) 1149
MwoI GCNNNNNNNGC 6 cut(s) 206, 215, 767, 844, 1062, 1203
NaeI GCCGGC 1 cut(s) 333
NarI GGCGCC 1 cut(s) 506
NciI CCSGG 3 cut(s) 264, 288, 975
NgoMIV GCCGGC 1 cut(s) 331
NlaIV GGNNCC 9 cut(s) 326, 344, 507, 558, 564, 609, 666, 711, 1057
NmeAIII GCCGAG 6 cut(s) 46, 825, 864, 873, 969, 1020
NmuCI GTSAC 1 cut(s) 31
PceI AGGCCT 1 cut(s) 1051
PciSI GCTCTTC 2 cut(s) 873, 981
PcsI WCGNNNNNNNCGW 5 cut(s) 48, 114, 507, 711, 1221
PdiI GCCGGC 1 cut(s) 333
PfeI GAWTC 6 cut(s) 371, 446, 512, 815, 1160, 1166
Pfl23II CGTACG 1 cut(s) 63
PflMI CCANNNNNTGG 3 cut(s) 572, 902, 1043
Ple19I CGATCG 1 cut(s) 169
PleI GAGTC 5 cut(s) 52, 130, 622, 724, 1220
PluTI GGCGCC 1 cut(s) 509
PpsI GAGTC 5 cut(s) 52, 130, 622, 724, 1220
Psp6I CCWGG 4 cut(s) 349, 1051, 1065, 1129
PspFI CCCAGC 1 cut(s) 327
PspGI CCWGG 4 cut(s) 349, 1051, 1065, 1129
PspLI CGTACG 1 cut(s) 63
PspN4I GGNNCC 9 cut(s) 326, 344, 507, 558, 564, 609, 666, 711, 1057
PspPI GGNCC 7 cut(s) 266, 324, 366, 441, 909, 1002, 1063
PsuI RGATCY 7 cut(s) 473, 524, 575, 626, 677, 728, 779
PvuI CGATCG 1 cut(s) 169
PvuII CAGCTG 1 cut(s) 770
SalI GTCGAC 1 cut(s) 537
SapI GCTCTTC 2 cut(s) 873, 981
Sau96I GGNCC 7 cut(s) 266, 324, 366, 441, 909, 1002, 1063
SchI GAGTC 5 cut(s) 52, 131, 623, 725, 1220
ScrFI CCNGG 7 cut(s) 264, 288, 351, 975, 1053, 1067, 1131
SduI GDGCHC 2 cut(s) 198, 459
SfaNI GCATC 1 cut(s) 668
SfoI GGCGCC 1 cut(s) 507
SinI GGWCC 2 cut(s) 266, 1002
Sse9I AATT 1 cut(s) 773
SseBI AGGCCT 1 cut(s) 1051
SspDI GGCGCC 1 cut(s) 505
StuI AGGCCT 1 cut(s) 1051
StyD4I CCNGG 7 cut(s) 262, 286, 349, 973, 1051, 1065, 1129
TaaI ACNGT 5 cut(s) 68, 536, 638, 809, 1187
TaqI TCGA 5 cut(s) 42, 108, 159, 538, 640
TasI AATT 1 cut(s) 773
TatI WGTACW 1 cut(s) 231
TauI GCSGC 9 cut(s) 102, 203, 838, 910, 994, 1024, 1128, 1152, 1260
TfiI GAWTC 6 cut(s) 371, 446, 512, 815, 1160, 1166
TscAI CASTG 1 cut(s) 768
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 1 cut(s) 1252
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 1 cut(s) 42
TspGWI ACGGA 2 cut(s) 51, 1230
TspRI CASTG 1 cut(s) 768
Van91I CCANNNNNTGG 3 cut(s) 572, 902, 1043
VpaK11BI GGWCC 2 cut(s) 266, 1002
XapI RAATTY 1 cut(s) 773
XcmI CCANNNNNNNNNTGG 1 cut(s) 335
XmiI GTMKAC 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.