Rh2DG619300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002017
Physical Location & Seq
Forward (+)
84932242 .. 84937078
4837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG619300.1

Sequence Viewer

Length: 1257 bp
ATGCCGTACTACTCGCACGTCGACGAAGTCCACGACTTCGACGAGTACGATCCGACCCCCTACGGCGGCGGATACGACCTGTTTCTCACCTACGGCCGCCCCCTCGAGCCTTCCGAGGAGACCTGCTACCCCAGCTCCTCCGATCAGGACGACTACGAACGCCCCGATCTCTCCTCCTACAGCGAGCCCTCCGCCTACGCCGATGAGGCCCTCAATAACGAGTACAGCAGCTACCAGCGTCGCAAGCCCCGACCCGGATTTGGTGGACGACCTGATCATGAGGAGGCTGCGGAAGGTGGAGGAGAGTATGGATCCAGGCCCAGTAGGCCTCAGTATGGATCCGAGGAGCCCGGATCGGAGTATGGATCCGGTTACGGGCGGAAACCCGAGGCCGAGCAGCCCAGTTCCGAGTACGGGTCGGGTTACGGTCGGAAGCCGGAGTACAAAGCGCCTGAATCGGAGTATGGATCGGGTTACGGGCGCAAACCCGAGTACGAGGCGCCGGAATCGGAGTATGGTCGGAAACCCGAGTACGAAGCGCCGGTAGGGGCGGGTTATGGCAGGAAACCCGATTACGAAGCGCCGGTCGGGTCGGGTTATGGCCGGAAGCCAAGTTATGGTGAGGATGAGAGTGGTGGATACGGCGATGAGCCGCCGAGGCGGAAGCCGAGCTATGGTGAGGAGAAACCCGAGTTTGAGGGTTCGGGTTATGGGGATGAACCGCCGAGGAGACCTGGGTTTGGAAGGCCTGGTGGTTATGGTGGGGAGGAGAGTGAAGAGAGGCCCAGTTATGGGAGGACTGAGTTTCAGGAGCCTGGTTATGGTGATGAGCCGCCGAGGAAGCCCAGCTATGGTGGTAGGACTAGTTATGAGACCCCGGAAACTGACGAGTATGGGAAGCCCAGTTATGGCAGGCCAAGCTATGAGACCCCGGAGACTGAGGAGTATGGGAAGCCCAACTATGGCAGGCCAAGCTATGAGACCCCGGAGACTGAGGAGTATGGGAAGCCCAGTTATGGGCGGTCTGAGGAGCAGGAGTACCGCAAGCCGAAGCCTAGCTATGGGAGGGGTAGTGATGATGAGGATTCTGAGCGTCGTCAGAGGCGTGGTAGTGATGATGAGGATTCTCAGCCTCGTCGGGGATATGGTGAAGAGGGCTATGGCCGCAAGAAATATGGAAATGATGACTCAGATGATGATGAGGAGAAAAAGCAGCGTCGCCACCACCACCATCGCAAGAGCTACGATGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

418

Amino Acids

47.36

Weight (kDa)

4.69

Isoelectric Point (pI)

60.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 131
AccB1I GGYRCC 1 cut(s) 499
AccB7I CCANNNNNTGG 1 cut(s) 617
AccI GTMKAC 1 cut(s) 21
AclWI GGATC 9 cut(s) 44, 306, 319, 333, 346, 360, 361, 373, 475
AcoI YGGCCR 3 cut(s) 94, 601, 1162
AcyI GRCGYC 1 cut(s) 500
AfaI GTAC 8 cut(s) 8, 47, 224, 413, 443, 494, 533, 1040
AhlI ACTAGT 1 cut(s) 863
AjiI CACGTC 1 cut(s) 19
AjnI CCWGG 4 cut(s) 314, 733, 748, 814
AluBI AGCT 8 cut(s) 135, 231, 672, 849, 921, 975, 1059, 1242
AluI AGCT 8 cut(s) 135, 231, 672, 849, 921, 975, 1059, 1242
Alw26I GTCTC 7 cut(s) 113, 724, 866, 920, 929, 974, 983
AlwI GGATC 9 cut(s) 44, 306, 319, 333, 346, 360, 361, 373, 475
Ama87I CYCGRG 5 cut(s) 104, 386, 488, 527, 689
ApeKI GCWGC 4 cut(s) 228, 287, 397, 1213
ArsI GACNNNNNNTTYG 2 cut(s) 570, 602
AspLEI GCGC 5 cut(s) 451, 483, 502, 541, 583
AspS9I GGNCC 3 cut(s) 208, 318, 783
AsuC2I CCSGG 5 cut(s) 255, 351, 878, 932, 986
AsuHPI GGTGA 5 cut(s) 79, 632, 689, 836, 1160
AvaI CYCGRG 5 cut(s) 104, 386, 488, 527, 689
BamHI GGATCC 3 cut(s) 311, 338, 365
BanI GGYRCC 1 cut(s) 499
BanII GRGCYC 2 cut(s) 189, 351
BarI GAAGNNNNNNTAC 2 cut(s) 425, 457
BbvI GCAGC 4 cut(s) 240, 274, 409, 1225
BccI CCATC 1 cut(s) 1239
BceAI ACGGC 3 cut(s) 79, 109, 658
BciT130I CCWGG 4 cut(s) 316, 735, 750, 816
BciVI GTATCC 2 cut(s) 65, 632
BclI TGATCA 1 cut(s) 274
BcnI CCSGG 5 cut(s) 255, 351, 878, 932, 986
BcoDI GTCTC 7 cut(s) 113, 724, 866, 920, 929, 974, 983
BcuI ACTAGT 1 cut(s) 863
BfaI CTAG 2 cut(s) 864, 1056
BfmI CTRYAG 1 cut(s) 178
BfoI RGCGCY 4 cut(s) 452, 503, 542, 584
BfuAI ACCTGC 1 cut(s) 131
BfuI GTATCC 2 cut(s) 65, 632
BglI GCCNNNNNGGC 3 cut(s) 206, 325, 658
BisI GCNGC 9 cut(s) 67, 97, 229, 288, 398, 653, 833, 1165, 1214
BlsI GCNGC 9 cut(s) 68, 98, 230, 289, 399, 654, 834, 1166, 1215
Bme1390I CCNGG 9 cut(s) 255, 316, 351, 735, 750, 816, 878, 932, 986
BmeT110I CYCGRG 5 cut(s) 104, 386, 488, 527, 689
BmgBI CACGTC 1 cut(s) 19
BmgT120I GGNCC 3 cut(s) 208, 318, 783
BmiI GGNNCC 6 cut(s) 313, 340, 348, 367, 501, 813
BmrFI CCNGG 9 cut(s) 255, 316, 351, 735, 750, 816, 878, 932, 986
BmrI ACTGGG 5 cut(s) 315, 396, 780, 897, 1005
BmuI ACTGGG 5 cut(s) 315, 396, 780, 897, 1005
BpuMI CCSGG 5 cut(s) 255, 351, 878, 932, 986
BsaHI GRCGYC 1 cut(s) 500
BsaI GGTCTC 5 cut(s) 113, 724, 866, 920, 974
BsaWI WCCGGW 1 cut(s) 368
BsaXI ACNNNNNCTCC 6 cut(s) 119, 149, 1022, 1052, 1057, 1087
Bse118I RCCGGY 2 cut(s) 541, 583
Bse1I ACTGG 5 cut(s) 321, 402, 786, 903, 1011
BseBI CCWGG 4 cut(s) 316, 735, 750, 816
BseGI GGATG 2 cut(s) 631, 721
BseMII CTCAG 8 cut(s) 344, 792, 930, 984, 1017, 1080, 1142, 1203
BseNI ACTGG 5 cut(s) 321, 402, 786, 903, 1011
BseX3I CGGCCG 1 cut(s) 94
BseXI GCAGC 4 cut(s) 240, 274, 409, 1225
BseYI CCCAGC 2 cut(s) 131, 845
Bsh1285I CGRYCG 3 cut(s) 97, 430, 588
BshNI GGYRCC 1 cut(s) 499
BsiEI CGRYCG 3 cut(s) 97, 430, 588
BsiHKCI CYCGRG 5 cut(s) 104, 386, 488, 527, 689
BsmAI GTCTC 7 cut(s) 113, 724, 866, 920, 929, 974, 983
Bso31I GGTCTC 5 cut(s) 113, 724, 866, 920, 974
BsoBI CYCGRG 5 cut(s) 104, 386, 488, 527, 689
Bsp1286I GDGCHC 2 cut(s) 189, 351
Bsp143I GATC 9 cut(s) 49, 142, 166, 274, 311, 338, 353, 365, 467
BspCNI CTCAG 8 cut(s) 343, 793, 931, 985, 1018, 1081, 1141, 1202
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 6 cut(s) 313, 340, 348, 367, 501, 813
BspMI ACCTGC 1 cut(s) 131
BspPI GGATC 9 cut(s) 44, 306, 319, 333, 346, 360, 361, 373, 475
BspT107I GGYRCC 1 cut(s) 499
BspTNI GGTCTC 5 cut(s) 113, 724, 866, 920, 974
BsrFI RCCGGY 2 cut(s) 541, 583
BsrI ACTGG 5 cut(s) 321, 402, 786, 903, 1011
BssAI RCCGGY 2 cut(s) 541, 583
BssMI GATC 9 cut(s) 49, 142, 166, 274, 311, 338, 353, 365, 467
BssNI GRCGYC 1 cut(s) 500
Bst2UI CCWGG 4 cut(s) 316, 735, 750, 816
Bst4CI ACNGT 1 cut(s) 428
Bst6I CTCTTC 2 cut(s) 771, 1146
BstACI GRCGYC 1 cut(s) 500
BstC8I GCNNGC 5 cut(s) 185, 245, 914, 968, 1046
BstDEI CTNAG 8 cut(s) 330, 801, 939, 993, 1026, 1089, 1128, 1189
BstF5I GGATG 2 cut(s) 631, 721
BstH2I RGCGCY 4 cut(s) 452, 503, 542, 584
BstHHI GCGC 5 cut(s) 451, 483, 502, 541, 583
BstKTI GATC 9 cut(s) 52, 145, 169, 277, 314, 341, 356, 368, 470
BstMAI GTCTC 7 cut(s) 113, 724, 866, 920, 929, 974, 983
BstMBI GATC 9 cut(s) 49, 142, 166, 274, 311, 338, 353, 365, 467
BstMCI CGRYCG 3 cut(s) 97, 430, 588
BstMWI GCNNNNNNNGC 9 cut(s) 132, 206, 244, 325, 658, 841, 918, 972, 1164
BstNI CCWGG 4 cut(s) 316, 735, 750, 816
BstSCI CCNGG 9 cut(s) 253, 314, 349, 733, 748, 814, 876, 930, 984
BstSFI CTRYAG 1 cut(s) 178
BstV1I GCAGC 4 cut(s) 240, 274, 409, 1225
BstX2I RGATCY 3 cut(s) 311, 338, 365
BstYI RGATCY 3 cut(s) 311, 338, 365
BstZI CGGCCG 1 cut(s) 94
BsuI GTATCC 2 cut(s) 65, 632
BtgZI GCGATG 2 cut(s) 660, 1217
BtrI CACGTC 1 cut(s) 19
BtsCI GGATG 2 cut(s) 631, 721
BveI ACCTGC 1 cut(s) 131
Cac8I GCNNGC 5 cut(s) 185, 245, 914, 968, 1046
CciI TCATGA 1 cut(s) 277
CfoI GCGC 5 cut(s) 451, 483, 502, 541, 583
Cfr10I RCCGGY 2 cut(s) 541, 583
Cfr13I GGNCC 3 cut(s) 208, 318, 783
CseI GACGC 3 cut(s) 227, 1082, 1205
Csp6I GTAC 8 cut(s) 7, 46, 223, 412, 442, 493, 532, 1039
CviAII CATG 1 cut(s) 278
CviQI GTAC 8 cut(s) 7, 46, 223, 412, 442, 493, 532, 1039
DdeI CTNAG 8 cut(s) 330, 801, 939, 993, 1026, 1089, 1128, 1189
DinI GGCGCC 1 cut(s) 501
DpnI GATC 9 cut(s) 51, 144, 168, 276, 313, 340, 355, 367, 469
DpnII GATC 9 cut(s) 49, 142, 166, 274, 311, 338, 353, 365, 467
EaeI YGGCCR 3 cut(s) 94, 601, 1162
EagI CGGCCG 1 cut(s) 94
Eam1104I CTCTTC 2 cut(s) 771, 1146
EarI CTCTTC 2 cut(s) 771, 1146
EciI GGCGGA 4 cut(s) 84, 181, 394, 676
EclXI CGGCCG 1 cut(s) 94
Eco147I AGGCCT 2 cut(s) 328, 748
Eco24I GRGCYC 2 cut(s) 189, 351
Eco31I GGTCTC 5 cut(s) 113, 724, 866, 920, 974
Eco52I CGGCCG 1 cut(s) 94
Eco88I CYCGRG 5 cut(s) 104, 386, 488, 527, 689
EcoO109I RGGNCCY 1 cut(s) 208
EcoRII CCWGG 4 cut(s) 314, 733, 748, 814
EcoT38I GRGCYC 2 cut(s) 189, 351
EgeI GGCGCC 1 cut(s) 501
EheI GGCGCC 1 cut(s) 501
FaeI CATG 1 cut(s) 281
FatI CATG 1 cut(s) 277
FauI CCCGC 1 cut(s) 544
FbaI TGATCA 1 cut(s) 274
FblI GTMKAC 1 cut(s) 21
Fnu4HI GCNGC 9 cut(s) 67, 97, 229, 288, 398, 653, 833, 1165, 1214
FokI GGATG 2 cut(s) 638, 728
FriOI GRGCYC 2 cut(s) 189, 351
Fsp4HI GCNGC 9 cut(s) 67, 97, 229, 288, 398, 653, 833, 1165, 1214
FspBI CTAG 2 cut(s) 864, 1056
GlaI GCGC 5 cut(s) 450, 482, 501, 540, 582
GluI GCNGC 9 cut(s) 67, 97, 229, 288, 398, 653, 833, 1165, 1214
GsaI CCCAGC 2 cut(s) 135, 849
HaeII RGCGCY 4 cut(s) 452, 503, 542, 584
HgaI GACGC 3 cut(s) 227, 1082, 1205
HhaI GCGC 5 cut(s) 451, 483, 502, 541, 583
Hin1I GRCGYC 1 cut(s) 500
Hin1II CATG 1 cut(s) 281
Hin6I GCGC 5 cut(s) 449, 481, 500, 539, 581
HinP1I GCGC 5 cut(s) 449, 481, 500, 539, 581
HincII GTYRAC 1 cut(s) 22
HindII GTYRAC 1 cut(s) 22
HinfI GANTC 5 cut(s) 455, 506, 1085, 1124, 1187
HphI GGTGA 5 cut(s) 79, 632, 689, 836, 1160
Hpy166II GTNNAC 3 cut(s) 22, 31, 266
Hpy188III TCNNGA 3 cut(s) 146, 278, 809
Hpy8I GTNNAC 3 cut(s) 22, 31, 266
Hpy99I CGWCG 7 cut(s) 23, 26, 44, 243, 1098, 1140, 1221
HpyAV CCTTC 3 cut(s) 120, 287, 738
HpyCH4III ACNGT 1 cut(s) 428
HpyCH4IV ACGT 1 cut(s) 18
HpyF10VI GCNNNNNNNGC 9 cut(s) 132, 206, 244, 325, 658, 841, 918, 972, 1164
HpyF3I CTNAG 8 cut(s) 330, 801, 939, 993, 1026, 1089, 1128, 1189
HpySE526I ACGT 1 cut(s) 18
Hsp92I GRCGYC 1 cut(s) 500
Hsp92II CATG 1 cut(s) 281
HspAI GCGC 5 cut(s) 449, 481, 500, 539, 581
KasI GGCGCC 1 cut(s) 499
Ksp22I TGATCA 1 cut(s) 274
Kzo9I GATC 9 cut(s) 49, 142, 166, 274, 311, 338, 353, 365, 467
LmnI GCTCC 4 cut(s) 140, 346, 811, 1030
Lsp1109I GCAGC 4 cut(s) 240, 274, 409, 1225
MaeI CTAG 2 cut(s) 864, 1056
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 3 cut(s) 371, 422, 473
MalI GATC 9 cut(s) 51, 144, 168, 276, 313, 340, 355, 367, 469
MboI GATC 9 cut(s) 49, 142, 166, 274, 311, 338, 353, 365, 467
MboII GAAGA 2 cut(s) 788, 1163
MflI RGATCY 3 cut(s) 311, 338, 365
MhlI GDGCHC 2 cut(s) 189, 351
Mly113I GGCGCC 1 cut(s) 500
MlyI GAGTC 1 cut(s) 1181
MmeI TCCRAC 3 cut(s) 77, 410, 500
MspR9I CCNGG 9 cut(s) 255, 316, 351, 735, 750, 816, 878, 932, 986
MvaI CCWGG 4 cut(s) 316, 735, 750, 816
MwoI GCNNNNNNNGC 9 cut(s) 132, 206, 244, 325, 658, 841, 918, 972, 1164
NarI GGCGCC 1 cut(s) 500
NciI CCSGG 5 cut(s) 255, 351, 878, 932, 986
NdeII GATC 9 cut(s) 49, 142, 166, 274, 311, 338, 353, 365, 467
NlaIII CATG 1 cut(s) 281
NlaIV GGNNCC 6 cut(s) 313, 340, 348, 367, 501, 813
NmeAIII GCCGAG 5 cut(s) 418, 681, 693, 750, 861
PaeR7I CTCGAG 1 cut(s) 104
PagI TCATGA 1 cut(s) 277
PceI AGGCCT 2 cut(s) 328, 748
PcsI WCGNNNNNNNCGW 7 cut(s) 30, 39, 45, 111, 162, 247, 1102
PfeI GAWTC 4 cut(s) 455, 506, 1085, 1124
PflFI GACNNNGTC 1 cut(s) 26
PflMI CCANNNNNTGG 1 cut(s) 617
PkrI GCNGC 9 cut(s) 68, 98, 230, 289, 399, 654, 834, 1166, 1215
PleI GAGTC 1 cut(s) 1181
PluTI GGCGCC 1 cut(s) 503
PpsI GAGTC 1 cut(s) 1181
Psp6I CCWGG 4 cut(s) 314, 733, 748, 814
PspFI CCCAGC 2 cut(s) 131, 845
PspGI CCWGG 4 cut(s) 314, 733, 748, 814
PspN4I GGNNCC 6 cut(s) 313, 340, 348, 367, 501, 813
PspPI GGNCC 3 cut(s) 208, 318, 783
PspXI VCTCGAGB 1 cut(s) 104
PsuI RGATCY 3 cut(s) 311, 338, 365
PsyI GACNNNGTC 1 cut(s) 26
RsaI GTAC 8 cut(s) 8, 47, 224, 413, 443, 494, 533, 1040
RsaNI GTAC 8 cut(s) 7, 46, 223, 412, 442, 493, 532, 1039
SalI GTCGAC 1 cut(s) 20
SatI GCNGC 9 cut(s) 67, 97, 229, 288, 398, 653, 833, 1165, 1214
Sau3AI GATC 9 cut(s) 49, 142, 166, 274, 311, 338, 353, 365, 467
Sau96I GGNCC 3 cut(s) 208, 318, 783
SchI GAGTC 1 cut(s) 1181
ScrFI CCNGG 9 cut(s) 255, 316, 351, 735, 750, 816, 878, 932, 986
SduI GDGCHC 2 cut(s) 189, 351
SfcI CTRYAG 1 cut(s) 178
SfiI GGCCNNNNNGGCC 1 cut(s) 325
SfoI GGCGCC 1 cut(s) 501
Sfr274I CTCGAG 1 cut(s) 104
SgrDI CGTCGACG 1 cut(s) 20
SlaI CTCGAG 1 cut(s) 104
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
SpeI ACTAGT 1 cut(s) 863
SseBI AGGCCT 2 cut(s) 328, 748
SspDI GGCGCC 1 cut(s) 499
SspMI CTAG 2 cut(s) 864, 1056
StuI AGGCCT 2 cut(s) 328, 748
StyD4I CCNGG 9 cut(s) 253, 314, 349, 733, 748, 814, 876, 930, 984
TaaI ACNGT 1 cut(s) 428
TaiI ACGT 1 cut(s) 21
TaqI TCGA 3 cut(s) 21, 39, 105
TatI WGTACW 2 cut(s) 222, 441
TauI GCSGC 5 cut(s) 69, 99, 655, 835, 1167
TfiI GAWTC 4 cut(s) 455, 506, 1085, 1124
TseI GCWGC 4 cut(s) 228, 287, 397, 1213
TspDTI ATGAA 1 cut(s) 732
Tth111I GACNNNGTC 1 cut(s) 26
Van91I CCANNNNNTGG 1 cut(s) 617
XhoI CTCGAG 1 cut(s) 104
XmiI GTMKAC 1 cut(s) 21
XspI CTAG 2 cut(s) 864, 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.