RLG00000021731

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002017
Physical Location & Seq
Reverse (-)
77594364 .. 77596284
1921 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021731

Sequence Viewer

Length: 1170 bp
ATGCCGTACTACTCGCACGACGACGAAGTCCACGACTTCGACGAGTACGATCCGACCCCCTACGGCGGTGGATACGACCTGTTTCTCACCTTCGGCCGCCCCCTCGAGCCTTCCGAGGAGACTTGCTACCCCAGCTCCTCCGATCAGGACGACTACGAACGACCCGATCTCTCCTCCTACAGCGAGCCCTCCGCCTACGCCGATGAGGCCCTCAATAACGAGTACAGCAGCTACCAGCGTCCCAAGCCCCGACCCGGATTTGGTGGACGACCCGATCATGATGAGGCTGCGGAAGGTGGAGGAGAGTATGGATCCGAGGAGCCCGGATCGGAGCATGGATCCGGTTACGGGCGGAAACCCGAGGCCGATCAGCCCAGTTCCGAGTACGGGCCGGGTTACGGTCGGAAGCCGGAGTACAAAGCGCCTGAATCGGAGTATGGATCGGGTTACGGGCGGAAACCCGAGTACGAGGCGCCGGAATCGGAGTATGGTCGGAAACCCGAGTACGAAGCGCCGGTCCGGGCGGGTTATGGCAGGAAACCCTATTACGAAGCGCCGGTCGGGTCGGGTTATGGTGAGGATGAGAGTGGTGGATACGGCGATGAGCCGCCGAGGCGGAAGCCGAGCTATGGTGAGGAGAGACCCGAGTTTGAGGGTTCGGGTTATGGGGATGAACCGCCGAGGAGGCCTGGGTTTGGAAGGCCTGGTGGTTATAGGGGAGAGGAGAGTGAGGTGAGGCCTAGTTATGGGAGGAGTGAGTTTCAGGAGCCTGGTTTTGGTGATGAGCCGCCGAACAGGCCTATCTATGGTGGGAGGACTAGTTATGAGTCCCCGGAAACTGAGGAGTATGGGAAGCCCAGCTACGGCAGGCCAAGCTATGAGACCCCGGAGACTGAGGAGTTTGGAAGGCCCGGTTATGGGCGGTCTGAGGAGCAGGAGTACCGCAAGCCGAAGCCTAGCTATGGGAGGGGTAGTGATGATGAGGATTCTGAACGTCGTCAGAGGCGTGGTAGTGATGATGAGGATTCTCAGCCTCGTCGGGGATATGGTGAAGAGGGCTATGGCCGCAAGAAATATGGAAATGATGACTCAGATGATGATGAGGAGAAAAAGCAACGTCGCCACCACCACCATCGCAAGAGCTACAATGATGAATTGTCGGATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

390

Amino Acids

44.13

Weight (kDa)

4.63

Isoelectric Point (pI)

62.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 472
AclWI GGATC 7 cut(s) 44, 306, 319, 333, 334, 346, 448
AcoI YGGCCR 2 cut(s) 94, 1063
AcyI GRCGYC 1 cut(s) 473
AfaI GTAC 8 cut(s) 8, 47, 224, 386, 416, 467, 506, 941
AhlI ACTAGT 1 cut(s) 818
AjnI CCWGG 3 cut(s) 688, 703, 769
AluBI AGCT 7 cut(s) 135, 231, 627, 861, 876, 960, 1143
AluI AGCT 7 cut(s) 135, 231, 627, 861, 876, 960, 1143
Alw26I GTCTC 4 cut(s) 113, 634, 875, 884
AlwI GGATC 7 cut(s) 44, 306, 319, 333, 334, 346, 448
Ama87I CYCGRG 5 cut(s) 104, 359, 461, 500, 644
ApeKI GCWGC 2 cut(s) 228, 287
ArsI GACNNNNNNTTYG 4 cut(s) 501, 533, 543, 575
AspLEI GCGC 4 cut(s) 424, 475, 514, 556
AspS9I GGNCC 4 cut(s) 208, 389, 517, 909
AsuC2I CCSGG 7 cut(s) 255, 324, 393, 521, 833, 887, 912
AsuHPI GGTGA 6 cut(s) 79, 587, 644, 745, 791, 1061
AvaI CYCGRG 5 cut(s) 104, 359, 461, 500, 644
AvaII GGWCC 1 cut(s) 517
BamHI GGATCC 2 cut(s) 311, 338
BanI GGYRCC 1 cut(s) 472
BanII GRGCYC 2 cut(s) 189, 324
BarI GAAGNNNNNNTAC 4 cut(s) 398, 430, 845, 877
BbvI GCAGC 2 cut(s) 240, 274
BccI CCATC 1 cut(s) 1140
BceAI ACGGC 3 cut(s) 79, 613, 880
BciT130I CCWGG 3 cut(s) 690, 705, 771
BciVI GTATCC 2 cut(s) 65, 587
BcnI CCSGG 7 cut(s) 255, 324, 393, 521, 833, 887, 912
BcoDI GTCTC 4 cut(s) 113, 634, 875, 884
BcuI ACTAGT 1 cut(s) 818
BfaI CTAG 3 cut(s) 741, 819, 957
BfmI CTRYAG 1 cut(s) 178
BfoI RGCGCY 4 cut(s) 425, 476, 515, 557
BfuI GTATCC 2 cut(s) 65, 587
BglI GCCNNNNNGGC 4 cut(s) 206, 613, 685, 796
BisI GCNGC 6 cut(s) 97, 229, 288, 608, 788, 1066
BlsI GCNGC 6 cut(s) 98, 230, 289, 609, 789, 1067
Bme18I GGWCC 1 cut(s) 517
BmeT110I CYCGRG 5 cut(s) 104, 359, 461, 500, 644
BmgT120I GGNCC 4 cut(s) 208, 389, 517, 909
BmiI GGNNCC 5 cut(s) 313, 321, 340, 474, 768
BmrI ACTGGG 1 cut(s) 369
BmuI ACTGGG 1 cut(s) 369
BpuMI CCSGG 7 cut(s) 255, 324, 393, 521, 833, 887, 912
BsaHI GRCGYC 1 cut(s) 473
BsaI GGTCTC 2 cut(s) 634, 875
BsaJI CCNNGG 8 cut(s) 114, 315, 360, 611, 680, 689, 831, 885
BsaWI WCCGGW 1 cut(s) 341
BsaXI ACNNNNNCTCC 8 cut(s) 119, 149, 805, 835, 923, 953, 958, 988
Bse118I RCCGGY 2 cut(s) 514, 556
Bse1I ACTGG 1 cut(s) 375
BseBI CCWGG 3 cut(s) 690, 705, 771
BseDI CCNNGG 8 cut(s) 114, 315, 360, 611, 680, 689, 831, 885
BseGI GGATG 2 cut(s) 586, 676
BseMII CTCAG 5 cut(s) 831, 885, 918, 1043, 1104
BseNI ACTGG 1 cut(s) 375
BseX3I CGGCCG 1 cut(s) 94
BseXI GCAGC 2 cut(s) 240, 274
BseYI CCCAGC 2 cut(s) 131, 857
Bsh1285I CGRYCG 3 cut(s) 97, 403, 561
BshNI GGYRCC 1 cut(s) 472
BsiEI CGRYCG 3 cut(s) 97, 403, 561
BsiHKCI CYCGRG 5 cut(s) 104, 359, 461, 500, 644
BslFI GGGAC 2 cut(s) 225, 814
BsmAI GTCTC 4 cut(s) 113, 634, 875, 884
BsmFI GGGAC 2 cut(s) 225, 814
Bso31I GGTCTC 2 cut(s) 634, 875
BsoBI CYCGRG 5 cut(s) 104, 359, 461, 500, 644
Bsp1286I GDGCHC 2 cut(s) 189, 324
Bsp143I GATC 9 cut(s) 49, 142, 166, 274, 311, 326, 338, 367, 440
BspCNI CTCAG 5 cut(s) 832, 886, 919, 1042, 1103
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 5 cut(s) 313, 321, 340, 474, 768
BspPI GGATC 7 cut(s) 44, 306, 319, 333, 334, 346, 448
BspT107I GGYRCC 1 cut(s) 472
BspTNI GGTCTC 2 cut(s) 634, 875
BsrFI RCCGGY 2 cut(s) 514, 556
BsrI ACTGG 1 cut(s) 375
BssAI RCCGGY 2 cut(s) 514, 556
BssECI CCNNGG 8 cut(s) 114, 315, 360, 611, 680, 689, 831, 885
BssMI GATC 9 cut(s) 49, 142, 166, 274, 311, 326, 338, 367, 440
BssNI GRCGYC 1 cut(s) 473
Bst2UI CCWGG 3 cut(s) 690, 705, 771
Bst4CI ACNGT 1 cut(s) 401
Bst6I CTCTTC 1 cut(s) 1047
BstACI GRCGYC 1 cut(s) 473
BstC8I GCNNGC 3 cut(s) 185, 869, 947
BstDEI CTNAG 5 cut(s) 840, 894, 927, 1029, 1090
BstF5I GGATG 2 cut(s) 586, 676
BstH2I RGCGCY 4 cut(s) 425, 476, 515, 557
BstHHI GCGC 4 cut(s) 424, 475, 514, 556
BstKTI GATC 9 cut(s) 52, 145, 169, 277, 314, 329, 341, 370, 443
BstMAI GTCTC 4 cut(s) 113, 634, 875, 884
BstMBI GATC 9 cut(s) 49, 142, 166, 274, 311, 326, 338, 367, 440
BstMCI CGRYCG 3 cut(s) 97, 403, 561
BstMWI GCNNNNNNNGC 8 cut(s) 132, 206, 244, 613, 685, 796, 873, 1065
BstNI CCWGG 3 cut(s) 690, 705, 771
BstSFI CTRYAG 1 cut(s) 178
BstV1I GCAGC 2 cut(s) 240, 274
BstX2I RGATCY 2 cut(s) 311, 338
BstYI RGATCY 2 cut(s) 311, 338
BstZI CGGCCG 1 cut(s) 94
BsuI GTATCC 2 cut(s) 65, 587
BtgZI GCGATG 2 cut(s) 615, 1118
BtsCI GGATG 2 cut(s) 586, 676
Cac8I GCNNGC 3 cut(s) 185, 869, 947
CciI TCATGA 1 cut(s) 277
CfoI GCGC 4 cut(s) 424, 475, 514, 556
Cfr10I RCCGGY 2 cut(s) 514, 556
Cfr13I GGNCC 4 cut(s) 208, 389, 517, 909
CpoI CGGWCCG 1 cut(s) 517
CseI GACGC 1 cut(s) 227
Csp6I GTAC 8 cut(s) 7, 46, 223, 385, 415, 466, 505, 940
CspI CGGWCCG 1 cut(s) 517
CviAII CATG 2 cut(s) 278, 335
CviQI GTAC 8 cut(s) 7, 46, 223, 385, 415, 466, 505, 940
DdeI CTNAG 5 cut(s) 840, 894, 927, 1029, 1090
DinI GGCGCC 1 cut(s) 474
DpnI GATC 9 cut(s) 51, 144, 168, 276, 313, 328, 340, 369, 442
DpnII GATC 9 cut(s) 49, 142, 166, 274, 311, 326, 338, 367, 440
DrdI GACNNNNNNGTC 1 cut(s) 26
DseDI GACNNNNNNGTC 1 cut(s) 26
EaeI YGGCCR 2 cut(s) 94, 1063
EagI CGGCCG 1 cut(s) 94
Eam1104I CTCTTC 1 cut(s) 1047
EarI CTCTTC 1 cut(s) 1047
EciI GGCGGA 4 cut(s) 181, 367, 469, 631
EclXI CGGCCG 1 cut(s) 94
Eco147I AGGCCT 4 cut(s) 688, 703, 739, 799
Eco24I GRGCYC 2 cut(s) 189, 324
Eco31I GGTCTC 2 cut(s) 634, 875
Eco47I GGWCC 1 cut(s) 517
Eco52I CGGCCG 1 cut(s) 94
Eco88I CYCGRG 5 cut(s) 104, 359, 461, 500, 644
EcoO109I RGGNCCY 1 cut(s) 208
EcoRII CCWGG 3 cut(s) 688, 703, 769
EcoT38I GRGCYC 2 cut(s) 189, 324
EgeI GGCGCC 1 cut(s) 474
EheI GGCGCC 1 cut(s) 474
FaeI CATG 2 cut(s) 281, 338
FaqI GGGAC 2 cut(s) 225, 814
FatI CATG 2 cut(s) 277, 334
FauI CCCGC 1 cut(s) 517
Fnu4HI GCNGC 6 cut(s) 97, 229, 288, 608, 788, 1066
FokI GGATG 2 cut(s) 593, 683
FriOI GRGCYC 2 cut(s) 189, 324
Fsp4HI GCNGC 6 cut(s) 97, 229, 288, 608, 788, 1066
FspBI CTAG 3 cut(s) 741, 819, 957
GlaI GCGC 4 cut(s) 423, 474, 513, 555
GluI GCNGC 6 cut(s) 97, 229, 288, 608, 788, 1066
GsaI CCCAGC 2 cut(s) 135, 861
HaeII RGCGCY 4 cut(s) 425, 476, 515, 557
HgaI GACGC 1 cut(s) 227
HhaI GCGC 4 cut(s) 424, 475, 514, 556
Hin1I GRCGYC 1 cut(s) 473
Hin1II CATG 2 cut(s) 281, 338
Hin6I GCGC 4 cut(s) 422, 473, 512, 554
HinP1I GCGC 4 cut(s) 422, 473, 512, 554
HinfI GANTC 6 cut(s) 428, 479, 827, 986, 1025, 1088
HphI GGTGA 6 cut(s) 79, 587, 644, 745, 791, 1061
Hpy166II GTNNAC 2 cut(s) 31, 266
Hpy188III TCNNGA 3 cut(s) 146, 278, 764
Hpy8I GTNNAC 2 cut(s) 31, 266
Hpy99I CGWCG 6 cut(s) 23, 26, 44, 999, 1041, 1122
HpyAV CCTTC 5 cut(s) 100, 120, 287, 693, 900
HpyCH4III ACNGT 1 cut(s) 401
HpyCH4IV ACGT 2 cut(s) 994, 1117
HpyF10VI GCNNNNNNNGC 8 cut(s) 132, 206, 244, 613, 685, 796, 873, 1065
HpyF3I CTNAG 5 cut(s) 840, 894, 927, 1029, 1090
HpySE526I ACGT 2 cut(s) 994, 1117
Hsp92I GRCGYC 1 cut(s) 473
Hsp92II CATG 2 cut(s) 281, 338
HspAI GCGC 4 cut(s) 422, 473, 512, 554
KasI GGCGCC 1 cut(s) 472
Kzo9I GATC 9 cut(s) 49, 142, 166, 274, 311, 326, 338, 367, 440
LmnI GCTCC 5 cut(s) 140, 319, 331, 766, 931
Lsp1109I GCAGC 2 cut(s) 240, 274
MaeI CTAG 3 cut(s) 741, 819, 957
MaeII ACGT 2 cut(s) 994, 1117
MaeIII GTNAC 3 cut(s) 344, 395, 446
MalI GATC 9 cut(s) 51, 144, 168, 276, 313, 328, 340, 369, 442
MboI GATC 9 cut(s) 49, 142, 166, 274, 311, 326, 338, 367, 440
MboII GAAGA 1 cut(s) 1064
MflI RGATCY 2 cut(s) 311, 338
MhlI GDGCHC 2 cut(s) 189, 324
MluCI AATT 1 cut(s) 1154
Mly113I GGCGCC 1 cut(s) 473
MlyI GAGTC 2 cut(s) 836, 1082
MmeI TCCRAC 4 cut(s) 77, 383, 473, 1140
MvaI CCWGG 3 cut(s) 690, 705, 771
MwoI GCNNNNNNNGC 8 cut(s) 132, 206, 244, 613, 685, 796, 873, 1065
NarI GGCGCC 1 cut(s) 473
NciI CCSGG 7 cut(s) 255, 324, 393, 521, 833, 887, 912
NdeII GATC 9 cut(s) 49, 142, 166, 274, 311, 326, 338, 367, 440
NlaIII CATG 2 cut(s) 281, 338
NlaIV GGNNCC 5 cut(s) 313, 321, 340, 474, 768
NmeAIII GCCGAG 3 cut(s) 636, 648, 705
PaeR7I CTCGAG 1 cut(s) 104
PagI TCATGA 1 cut(s) 277
PceI AGGCCT 4 cut(s) 688, 703, 739, 799
PcsI WCGNNNNNNNCGW 6 cut(s) 30, 39, 45, 111, 162, 1003
PfeI GAWTC 4 cut(s) 428, 479, 986, 1025
PflFI GACNNNGTC 1 cut(s) 26
PkrI GCNGC 6 cut(s) 98, 230, 289, 609, 789, 1067
PleI GAGTC 2 cut(s) 835, 1082
PluTI GGCGCC 1 cut(s) 476
PpsI GAGTC 2 cut(s) 835, 1082
Psp6I CCWGG 3 cut(s) 688, 703, 769
PspFI CCCAGC 2 cut(s) 131, 857
PspGI CCWGG 3 cut(s) 688, 703, 769
PspN4I GGNNCC 5 cut(s) 313, 321, 340, 474, 768
PspPI GGNCC 4 cut(s) 208, 389, 517, 909
PspXI VCTCGAGB 1 cut(s) 104
PsuI RGATCY 2 cut(s) 311, 338
PsyI GACNNNGTC 1 cut(s) 26
RsaI GTAC 8 cut(s) 8, 47, 224, 386, 416, 467, 506, 941
RsaNI GTAC 8 cut(s) 7, 46, 223, 385, 415, 466, 505, 940
Rsr2I CGGWCCG 1 cut(s) 517
RsrII CGGWCCG 1 cut(s) 517
SatI GCNGC 6 cut(s) 97, 229, 288, 608, 788, 1066
Sau3AI GATC 9 cut(s) 49, 142, 166, 274, 311, 326, 338, 367, 440
Sau96I GGNCC 4 cut(s) 208, 389, 517, 909
SchI GAGTC 2 cut(s) 836, 1082
SduI GDGCHC 2 cut(s) 189, 324
SfcI CTRYAG 1 cut(s) 178
SfoI GGCGCC 1 cut(s) 474
Sfr274I CTCGAG 1 cut(s) 104
SinI GGWCC 1 cut(s) 517
SlaI CTCGAG 1 cut(s) 104
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
SpeI ACTAGT 1 cut(s) 818
Sse9I AATT 1 cut(s) 1154
SseBI AGGCCT 4 cut(s) 688, 703, 739, 799
SspDI GGCGCC 1 cut(s) 472
SspMI CTAG 3 cut(s) 741, 819, 957
StuI AGGCCT 4 cut(s) 688, 703, 739, 799
TaaI ACNGT 1 cut(s) 401
TaiI ACGT 2 cut(s) 997, 1120
TaqI TCGA 2 cut(s) 39, 105
TasI AATT 1 cut(s) 1154
TatI WGTACW 2 cut(s) 222, 414
TauI GCSGC 4 cut(s) 99, 610, 790, 1068
TfiI GAWTC 4 cut(s) 428, 479, 986, 1025
TseI GCWGC 2 cut(s) 228, 287
TspDTI ATGAA 2 cut(s) 687, 1167
Tth111I GACNNNGTC 1 cut(s) 26
VpaK11BI GGWCC 1 cut(s) 517
XhoI CTCGAG 1 cut(s) 104
XspI CTAG 3 cut(s) 741, 819, 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.