RchiOBHm_Chr1g0331211

(E,E)-alpha-farnesene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002039
Physical Location & Seq
Forward (+)
21054333 .. 21056945
2613 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1752 bp
ATGGATTCTAGTATGCACTTGCAAGATCCTCAAGTTGATGAGCAACAGATACTGCAATGGCAAACGAAATCTGAACCCTCTTACTTTGTACATGATCAAAGACGATCTGCAAATTACAAGCCAAATATTTGGAAGTATGAGTTCCTCGAATCCCTTAACAGCAAATTTGATGGAGAATGTTATCGAATTCAAATTCAGAAGCTAATAGAAGATGTAAAGGAAATGTTTGCTAATTGTAAGGAATCAGATTTAATAACCAATTTGGAGCTGCTTGACAGCATCCAAAAACTAGGCCTGAACAACCATTTTGGAAAGGAAATCAAGAAAGTCCTAGACACCATAACATCTGTTGAACTCGAAAATAACAGCAATCCTTACATTAGTTCAAGAGGTGATCTCTATGCTGCTGCACTACTCTTTAAGATTCTTAGGCAGCATGGTTATAAAGTTTCACAAGATATATTTGGTGGTTTGATGGATGAAATGGGTACATTAAAGAAGAAAACATTTGGGGATGTGAAAGGAATGCTTGAAATGTTGGAGGCCTCAAACCTAGCTTTAGAAGGTGAAAATATCCTAGAGGAGGCTAAAGCTTTCTTAATGGTCACTCTCAGAGATACCAATACTAGGTGTGATGATATAGACAATTGCACTTTCAAGCATGTGACTTATGCCCTGGAGCTTCCATCACAAAGGAGAGTGCAGTGGTTTAATGTGAAATGGCACATCAAATCCTACGAGAAAGACCATATTGATCAAGCCAACATGACCACTTTACTTGAGTTGGCTAAACTTAACTTCAACATGGTTCAAGCCACACTCCAAAAAGATCTAAGGGAAGTATCCATGTGGTGGAACAATCTAGGTCTCAATAAGAACTTGGACTTTGCAAGAGATAGACTGGTCGAATGTTTCATGTGTGCCGTGGGATTAGCTTTCGAGCCTGAGTACAAGTCCTTTAGAAAATGGATAACTAAAGTCGTCAATTTAATTCTCATCATCGATGACGTTTATGATGTTTATGGCTCATTAGAAGAGCTAAAACATTTCACCAATGCTGTTAAAAGGTGGGATATTAGGGAAACTGAGCAGCTTCCAGAGTGTATGAAGATCTGTTTCCAAGTACTCTACAATACTACTTGTGAAATTGCTTATGAAATTGAGGAGGAGAAAGGTTGGAACCAAGTGTTACCTCATTTGCGGAAAGTGTGGGGAGATTTCTGTAAAGCATTATTGGTGGAGGCAGAGTGGTACAATAAGGCCTACACACCGTCCTTTGAAGAACACCTTAGCAATGGTTGCATTTCATCATCTGCTTTGCTGACTTTGACTCATGCCTTCTTCTCCATGACACGTGCTGAAGGGGCCAAAAACATTGCTGATTTTCTGCACAAGAATGAAGATCTTGTGTACAATATCTCTTTGATAGTTCGACTCTTAAATGATCTGGGAACTTCTGTGGCTGAGCAAGAGAGAGGTGATGTTGCTTCATCAATCCTATGTTACATGAGAGAAGCGAATGCTTCAGAAGAAATAGCTCGAAAGCACATCAAAGGCATGATAGACAATGCATGGAAGAAAATAAACGAAAAATGCTTCACTCAAATGCCTGAAATATCTTCATTCTTCAACATTATGACAAATATAGCTCGGATTGGGCATAGCCTTTATCAAGATGGAGATGGATTTGGTGATCAAGAGCACGGGACTCGGGGACAGATCCAATCTTTACTAGTTGAACCTCTCTTGTAA

Protein Analysis

583

Amino Acids

67.49

Weight (kDa)

5.28

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 43 - 226 1.4e-40 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 287 - 527 3.6e-89 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 326 - 527 1.8e-54 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 444
AccB7I CCANNNNNTGG 1 cut(s) 852
AciI CCGC 1 cut(s) 1201
AclWI GGATC 2 cut(s) 20, 1714
AcsI RAATTY 3 cut(s) 164, 186, 192
AcuI CTGAAG 2 cut(s) 1380, 1509
AcvI CACGTG 1 cut(s) 1355
AfaI GTAC 6 cut(s) 90, 490, 950, 1125, 1253, 1412
AfiI CCNNNNNNNGG 3 cut(s) 583, 627, 852
AflIII ACRYGT 1 cut(s) 1352
AhlI ACTAGT 1 cut(s) 1732
AjnI CCWGG 1 cut(s) 675
Alw21I GWGCWC 1 cut(s) 1704
Alw26I GTCTC 1 cut(s) 872
AlwI GGATC 2 cut(s) 20, 1714
AlwNI CAGNNNCTG 1 cut(s) 52
Ama87I CYCGRG 1 cut(s) 1710
AoxI GGCC 4 cut(s) 292, 543, 1260, 1365
ApeKI GCWGC 5 cut(s) 268, 404, 407, 433, 1090
ApoI RAATTY 3 cut(s) 164, 186, 192
AspS9I GGNCC 1 cut(s) 1365
AsuHPI GGTGA 5 cut(s) 404, 578, 1042, 1490, 1703
AvaI CYCGRG 1 cut(s) 1710
BbrPI CACGTG 1 cut(s) 1355
Bbv12I GWGCWC 1 cut(s) 1704
BbvI GCAGC 5 cut(s) 255, 391, 394, 445, 1102
BccI CCATC 5 cut(s) 164, 469, 694, 1670, 1676
BceAI ACGGC 1 cut(s) 908
BcgI CGANNNNNNTGC 2 cut(s) 1691, 1725
BciT130I CCWGG 1 cut(s) 677
BciVI GTATCC 1 cut(s) 853
BclI TGATCA 3 cut(s) 94, 754, 1693
BcoDI GTCTC 1 cut(s) 872
BcuI ACTAGT 1 cut(s) 1732
BfaI CTAG 8 cut(s) 9, 290, 332, 554, 578, 627, 863, 1733
BfuI GTATCC 1 cut(s) 853
BglII AGATCT 3 cut(s) 829, 1110, 1402
BisI GCNGC 5 cut(s) 269, 405, 408, 434, 1091
BlpI GCTNAGC 1 cut(s) 1464
BlsI GCNGC 5 cut(s) 270, 406, 409, 435, 1092
BmcAI AGTACT 1 cut(s) 1125
Bme1390I CCNGG 1 cut(s) 677
BmeT110I CYCGRG 1 cut(s) 1710
BmgT120I GGNCC 1 cut(s) 1365
BmiI GGNNCC 2 cut(s) 1181, 1366
BmrFI CCNGG 1 cut(s) 677
BmsI GCATC 1 cut(s) 288
BplI GAGNNNNNCTC 2 cut(s) 381, 413
BpmI CTGGAG 1 cut(s) 698
Bpu10I CCTNAGC 1 cut(s) 1289
Bpu1102I GCTNAGC 1 cut(s) 1464
BpuEI CTTGAG 2 cut(s) 15, 800
Bsa29I ATCGAT 1 cut(s) 1002
BsaAI YACGTR 1 cut(s) 1355
BsaI GGTCTC 1 cut(s) 872
BsaJI CCNNGG 2 cut(s) 675, 924
BsaXI ACNNNNNCTCC 2 cut(s) 1232, 1262
Bsc4I CCNNNNNNNGG 3 cut(s) 583, 627, 852
Bse1I ACTGG 1 cut(s) 906
Bse3DI GCAATG 3 cut(s) 62, 1300, 1374
BseBI CCWGG 1 cut(s) 677
BseCI ATCGAT 1 cut(s) 1002
BseDI CCNNGG 2 cut(s) 675, 924
BseGI GGATG 3 cut(s) 279, 484, 520
BseLI CCNNNNNNNGG 3 cut(s) 583, 627, 852
BseMI GCAATG 3 cut(s) 62, 1300, 1374
BseMII CTCAG 4 cut(s) 625, 936, 1077, 1455
BseNI ACTGG 1 cut(s) 906
BseRI GAGGAG 3 cut(s) 596, 1178, 1181
BseXI GCAGC 5 cut(s) 255, 391, 394, 445, 1102
BsgI GTGCAG 3 cut(s) 393, 722, 1373
BshFI GGCC 4 cut(s) 294, 545, 1262, 1367
BshVI ATCGAT 1 cut(s) 1002
BsiHKAI GWGCWC 1 cut(s) 1704
BsiHKCI CYCGRG 1 cut(s) 1710
BslFI GGGAC 2 cut(s) 1720, 1728
BslI CCNNNNNNNGG 3 cut(s) 583, 627, 852
BsmAI GTCTC 1 cut(s) 872
BsmFI GGGAC 2 cut(s) 1720, 1728
BsmI GAATGC 2 cut(s) 531, 1525
BsnI GGCC 4 cut(s) 294, 545, 1262, 1367
Bso31I GGTCTC 1 cut(s) 872
BsoBI CYCGRG 1 cut(s) 1710
Bsp1286I GDGCHC 1 cut(s) 1704
Bsp1407I TGTACA 2 cut(s) 88, 1410
Bsp1720I GCTNAGC 1 cut(s) 1464
BspACI CCGC 1 cut(s) 1201
BspANI GGCC 4 cut(s) 294, 545, 1262, 1367
BspCNI CTCAG 4 cut(s) 624, 937, 1078, 1456
BspDI ATCGAT 1 cut(s) 1002
BspLI GGNNCC 2 cut(s) 1181, 1366
BspPI GGATC 2 cut(s) 20, 1714
BspQI GCTCTTC 1 cut(s) 1029
BspTNI GGTCTC 1 cut(s) 872
BsrDI GCAATG 3 cut(s) 62, 1300, 1374
BsrGI TGTACA 2 cut(s) 88, 1410
BsrI ACTGG 1 cut(s) 906
BssECI CCNNGG 2 cut(s) 675, 924
Bst2UI CCWGG 1 cut(s) 677
Bst4CI ACNGT 1 cut(s) 1272
Bst6I CTCTTC 1 cut(s) 1029
BstAPI GCANNNNNTGC 1 cut(s) 1299
BstAUI TGTACA 2 cut(s) 88, 1410
BstBAI YACGTR 1 cut(s) 1355
BstDEI CTNAG 7 cut(s) 428, 611, 833, 945, 1086, 1289, 1464
BstDSI CCRYGG 1 cut(s) 924
BstENI CCTNNNNNAGG 1 cut(s) 581
BstF5I GGATG 3 cut(s) 279, 484, 520
BstMAI GTCTC 1 cut(s) 872
BstMWI GCNNNNNNNGC 2 cut(s) 1299, 1364
BstNI CCWGG 1 cut(s) 677
BstNSI RCATGY 1 cut(s) 665
BstSCI CCNGG 1 cut(s) 675
BstV1I GCAGC 5 cut(s) 255, 391, 394, 445, 1102
BstX2I RGATCY 5 cut(s) 25, 829, 1110, 1402, 1719
BstXI CCANNNNNNTGG 1 cut(s) 129
BstYI RGATCY 5 cut(s) 25, 829, 1110, 1402, 1719
Bsu15I ATCGAT 1 cut(s) 1002
BsuI GTATCC 1 cut(s) 853
BsuRI GGCC 4 cut(s) 294, 545, 1262, 1367
BsuTUI ATCGAT 1 cut(s) 1002
BtgI CCRYGG 1 cut(s) 924
BtsCI GGATG 3 cut(s) 279, 484, 520
BtsI GCAGTG 1 cut(s) 710
BtsIMutI CAGTG 1 cut(s) 710
CaiI CAGNNNCTG 1 cut(s) 52
Cfr13I GGNCC 1 cut(s) 1365
ClaI ATCGAT 1 cut(s) 1002
Csp6I GTAC 6 cut(s) 89, 489, 949, 1124, 1252, 1411
CspCI CAANNNNNGTGG 2 cut(s) 760, 795
CviQI GTAC 6 cut(s) 89, 489, 949, 1124, 1252, 1411
DdeI CTNAG 7 cut(s) 428, 611, 833, 945, 1086, 1289, 1464
Eam1104I CTCTTC 1 cut(s) 1029
EarI CTCTTC 1 cut(s) 1029
Eco147I AGGCCT 3 cut(s) 294, 545, 1262
Eco31I GGTCTC 1 cut(s) 872
Eco57I CTGAAG 2 cut(s) 1380, 1509
Eco72I CACGTG 1 cut(s) 1355
Eco88I CYCGRG 1 cut(s) 1710
EcoNI CCTNNNNNAGG 1 cut(s) 581
EcoRI GAATTC 1 cut(s) 186
EcoRII CCWGG 1 cut(s) 675
EcoT22I ATGCAT 1 cut(s) 1573
FalI AAGNNNNNCTT 4 cut(s) 513, 545, 1272, 1304
FaqI GGGAC 2 cut(s) 1720, 1728
FbaI TGATCA 3 cut(s) 94, 754, 1693
Fnu4HI GCNGC 5 cut(s) 269, 405, 408, 434, 1091
FokI GGATG 3 cut(s) 266, 491, 527
Fsp4HI GCNGC 5 cut(s) 269, 405, 408, 434, 1091
FspBI CTAG 8 cut(s) 9, 290, 332, 554, 578, 627, 863, 1733
GluI GCNGC 5 cut(s) 269, 405, 408, 434, 1091
GsuI CTGGAG 1 cut(s) 698
HaeIII GGCC 4 cut(s) 294, 545, 1262, 1367
HindIII AAGCTT 1 cut(s) 591
HinfI GANTC 7 cut(s) 5, 149, 242, 424, 1330, 1434, 1708
HphI GGTGA 5 cut(s) 404, 578, 1042, 1490, 1703
Hpy166II GTNNAC 1 cut(s) 1411
Hpy188I TCNGA 6 cut(s) 73, 198, 247, 614, 1528, 1653
Hpy188III TCNNGA 5 cut(s) 322, 387, 1097, 1673, 1697
Hpy8I GTNNAC 1 cut(s) 1411
HpyAV CCTTC 3 cut(s) 557, 1348, 1355
HpyCH4III ACNGT 1 cut(s) 1272
HpyCH4IV ACGT 2 cut(s) 1008, 1354
HpyF10VI GCNNNNNNNGC 2 cut(s) 1299, 1364
HpyF3I CTNAG 7 cut(s) 428, 611, 833, 945, 1086, 1289, 1464
HpySE526I ACGT 2 cut(s) 1008, 1354
Ksp22I TGATCA 3 cut(s) 94, 754, 1693
LguI GCTCTTC 1 cut(s) 1029
LmnI GCTCC 2 cut(s) 265, 679
LpnPI CCDG 8 cut(s) 308, 662, 689, 887, 957, 1110, 1433, 1623
Lsp1109I GCAGC 5 cut(s) 255, 391, 394, 445, 1102
LweI GCATC 1 cut(s) 288
MaeI CTAG 8 cut(s) 9, 290, 332, 554, 578, 627, 863, 1733
MaeII ACGT 2 cut(s) 1008, 1354
MaeIII GTNAC 4 cut(s) 604, 664, 1188, 1502
MfeI CAATTG 1 cut(s) 646
MflI RGATCY 5 cut(s) 25, 829, 1110, 1402, 1719
MhlI GDGCHC 1 cut(s) 1704
MlyI GAGTC 3 cut(s) 1324, 1428, 1702
MmeI TCCRAC 2 cut(s) 519, 1157
Mph1103I ATGCAT 1 cut(s) 1573
MslI CAYNNNNRTG 2 cut(s) 1395, 1604
MspR9I CCNGG 1 cut(s) 677
MunI CAATTG 1 cut(s) 646
Mva1269I GAATGC 2 cut(s) 531, 1525
MvaI CCWGG 1 cut(s) 677
MwoI GCNNNNNNNGC 2 cut(s) 1299, 1364
NlaIV GGNNCC 2 cut(s) 1181, 1366
NmuCI GTSAC 2 cut(s) 604, 664
NsiI ATGCAT 1 cut(s) 1573
NspI RCATGY 1 cut(s) 665
PceI AGGCCT 3 cut(s) 294, 545, 1262
PciSI GCTCTTC 1 cut(s) 1029
PctI GAATGC 2 cut(s) 531, 1525
PfeI GAWTC 4 cut(s) 5, 149, 242, 424
PflMI CCANNNNNTGG 1 cut(s) 852
PkrI GCNGC 5 cut(s) 270, 406, 409, 435, 1092
PleI GAGTC 3 cut(s) 1324, 1428, 1702
PmaCI CACGTG 1 cut(s) 1355
PmlI CACGTG 1 cut(s) 1355
PpsI GAGTC 3 cut(s) 1324, 1428, 1702
Ppu21I YACGTR 1 cut(s) 1355
PsiI TTATAA 1 cut(s) 444
Psp6I CCWGG 1 cut(s) 675
PspCI CACGTG 1 cut(s) 1355
PspGI CCWGG 1 cut(s) 675
PspN4I GGNNCC 2 cut(s) 1181, 1366
PspPI GGNCC 1 cut(s) 1365
PstNI CAGNNNCTG 1 cut(s) 52
PsuI RGATCY 5 cut(s) 25, 829, 1110, 1402, 1719
RsaI GTAC 6 cut(s) 90, 490, 950, 1125, 1253, 1412
RsaNI GTAC 6 cut(s) 89, 489, 949, 1124, 1252, 1411
RseI CAYNNNNRTG 2 cut(s) 1395, 1604
SapI GCTCTTC 1 cut(s) 1029
SatI GCNGC 5 cut(s) 269, 405, 408, 434, 1091
Sau96I GGNCC 1 cut(s) 1365
ScaI AGTACT 1 cut(s) 1125
SchI GAGTC 3 cut(s) 1324, 1428, 1702
ScrFI CCNGG 1 cut(s) 677
SduI GDGCHC 1 cut(s) 1704
SfaNI GCATC 1 cut(s) 288
SmiMI CAYNNNNRTG 2 cut(s) 1395, 1604
SmlI CTYRAG 2 cut(s) 30, 779
SmoI CTYRAG 2 cut(s) 30, 779
SpeI ACTAGT 1 cut(s) 1732
SseBI AGGCCT 3 cut(s) 294, 545, 1262
SsiI CCGC 1 cut(s) 1201
SspI AATATT 1 cut(s) 127
SspMI CTAG 8 cut(s) 9, 290, 332, 554, 578, 627, 863, 1733
StuI AGGCCT 3 cut(s) 294, 545, 1262
StyD4I CCNGG 1 cut(s) 675
TaaI ACNGT 1 cut(s) 1272
TaiI ACGT 2 cut(s) 1011, 1357
TaqI TCGA 8 cut(s) 147, 184, 357, 906, 939, 1002, 1432, 1540
TatI WGTACW 4 cut(s) 88, 948, 1123, 1410
TfiI GAWTC 4 cut(s) 5, 149, 242, 424
TscAI CASTG 1 cut(s) 710
TseFI GTSAC 2 cut(s) 604, 664
TseI GCWGC 5 cut(s) 268, 404, 407, 433, 1090
Tsp45I GTSAC 2 cut(s) 604, 664
TspDTI ATGAA 8 cut(s) 495, 904, 1121, 1170, 1296, 1413, 1479, 1611
TspRI CASTG 1 cut(s) 710
Van91I CCANNNNNTGG 1 cut(s) 852
XagI CCTNNNNNAGG 1 cut(s) 581
XapI RAATTY 3 cut(s) 164, 186, 192
XceI RCATGY 1 cut(s) 665
XspI CTAG 8 cut(s) 9, 290, 332, 554, 578, 627, 863, 1733
ZrmI AGTACT 1 cut(s) 1125
Zsp2I ATGCAT 1 cut(s) 1573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.