RchiOBHm_Chr4g0403991

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001930
Physical Location & Seq
Forward (+)
23498498 .. 23499187
690 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37562

Sequence Viewer

Length: 624 bp
ATGGACTCAATGGTAAGGCCATATATACCAATGTCAAAAGAACTTGAAGACTTGGTGTTGCGAATGAAAAGATTCAGGTTCAGAAAATCAAGCAAGATTTCTGGTGTAAACCTCTTTGGGTTGTGGGCATATGATACAGTTTGGGCATTAGCAATGGCAGTGGAGAAGGTTGGAATAGCAAGCAGCGTTGATCCTGCAAGTTTGGGAACTGGTCAGAATCTTCTTGAAGCTATCCTAGATTTAAAATTTGAAAACCTCAGTGGGAATTTCCGAATAATTGGATATTGGAACCAAAAGAAAGGACTTTCGCGAAAGCTGAATGGAGCAACAGACTTGGATAAAAGGAAGACACTTGAGAAACCAATCTGGCCAGGGTACACTACAGACATCCCTCCAACAAAGAAATTGAAGATTGGAGTTCCCTTGAAACAGGGTTTTAATGAATTCCTAAAATTCGAAAACAACCACCATATATCAGGATTCTCTGCTGATATATTCTTTGCTGCAGTAAAGCAACTGCCATTTTCCCTTTCATATGAGTTTTCTAACTTTTCGGGGACTTATGATGAACTTCTTTATCAAATTTATCTTCAGGCAAGTTTTAATTACAAACTACTGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

207

Amino Acids

23.61

Weight (kDa)

9.43

Isoelectric Point (pI)

21.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANF_receptor PF01094 5 - 92 1.3e-09 Receptor family ligand binding region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 310
AclWI GGATC 1 cut(s) 185
AcoI YGGCCR 1 cut(s) 368
AcsI RAATTY 5 cut(s) 245, 265, 443, 452, 582
AcuI CTGAAG 1 cut(s) 575
AfaI GTAC 1 cut(s) 377
AgsI TTSAA 5 cut(s) 47, 227, 251, 409, 427
AjnI CCWGG 1 cut(s) 370
AluBI AGCT 2 cut(s) 230, 316
AluI AGCT 2 cut(s) 230, 316
AlwI GGATC 1 cut(s) 185
AoxI GGCC 2 cut(s) 17, 368
ApeKI GCWGC 2 cut(s) 183, 503
ApoI RAATTY 5 cut(s) 245, 265, 443, 452, 582
Asp700I GAANNNNTTC 1 cut(s) 71
AsuII TTCGAA 1 cut(s) 456
BalI TGGCCA 1 cut(s) 370
BbsI GAAGAC 2 cut(s) 54, 353
BbvI GCAGC 2 cut(s) 195, 490
BciT130I CCWGG 1 cut(s) 372
BfaI CTAG 1 cut(s) 236
BfmI CTRYAG 2 cut(s) 381, 504
BisI GCNGC 2 cut(s) 184, 504
BlsI GCNGC 2 cut(s) 185, 505
Bme1390I CCNGG 1 cut(s) 372
BmiI GGNNCC 1 cut(s) 290
BmrFI CCNGG 1 cut(s) 372
BpiI GAAGAC 2 cut(s) 54, 353
Bpu14I TTCGAA 1 cut(s) 456
BpuEI CTTGAG 1 cut(s) 374
BsaJI CCNNGG 1 cut(s) 371
Bse1I ACTGG 1 cut(s) 214
Bse3DI GCAATG 1 cut(s) 159
BseBI CCWGG 1 cut(s) 372
BseDI CCNNGG 1 cut(s) 371
BseGI GGATG 1 cut(s) 387
BseMI GCAATG 1 cut(s) 159
BseMII CTCAG 2 cut(s) 271, 608
BseNI ACTGG 1 cut(s) 214
BseXI GCAGC 2 cut(s) 195, 490
Bsh1236I CGCG 1 cut(s) 310
BshFI GGCC 2 cut(s) 19, 370
BslFI GGGAC 1 cut(s) 571
BsmFI GGGAC 1 cut(s) 571
BsnI GGCC 2 cut(s) 19, 370
Bsp119I TTCGAA 1 cut(s) 456
Bsp143I GATC 1 cut(s) 190
Bsp68I TCGCGA 1 cut(s) 310
BspANI GGCC 2 cut(s) 19, 370
BspCNI CTCAG 2 cut(s) 270, 609
BspFNI CGCG 1 cut(s) 310
BspLI GGNNCC 1 cut(s) 290
BspMAI CTGCAG 1 cut(s) 508
BspPI GGATC 1 cut(s) 185
BspT104I TTCGAA 1 cut(s) 456
BsrDI GCAATG 1 cut(s) 159
BsrI ACTGG 1 cut(s) 214
BssECI CCNNGG 1 cut(s) 371
BssMI GATC 1 cut(s) 190
Bst2UI CCWGG 1 cut(s) 372
Bst4CI ACNGT 1 cut(s) 139
BstBI TTCGAA 1 cut(s) 456
BstC8I GCNNGC 1 cut(s) 181
BstDEI CTNAG 2 cut(s) 257, 617
BstF5I GGATG 1 cut(s) 387
BstFNI CGCG 1 cut(s) 310
BstKTI GATC 1 cut(s) 193
BstMBI GATC 1 cut(s) 190
BstNI CCWGG 1 cut(s) 372
BstSCI CCNGG 1 cut(s) 370
BstSFI CTRYAG 2 cut(s) 381, 504
BstUI CGCG 1 cut(s) 310
BstV1I GCAGC 2 cut(s) 195, 490
BstV2I GAAGAC 2 cut(s) 54, 353
BsuRI GGCC 2 cut(s) 19, 370
BtsCI GGATG 1 cut(s) 387
BtsI GCAGTG 1 cut(s) 165
BtsIMutI CAGTG 2 cut(s) 165, 265
BtuMI TCGCGA 1 cut(s) 310
Cac8I GCNNGC 1 cut(s) 181
Csp6I GTAC 1 cut(s) 376
CspCI CAANNNNNGTGG 2 cut(s) 141, 176
CviJI RGCY 4 cut(s) 19, 230, 316, 370
CviKI_1 RGCY 4 cut(s) 19, 230, 316, 370
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 2 cut(s) 257, 617
DpnI GATC 1 cut(s) 192
DpnII GATC 1 cut(s) 190
DraI TTTAAA 1 cut(s) 243
EaeI YGGCCR 1 cut(s) 368
Eco57I CTGAAG 1 cut(s) 575
EcoRI GAATTC 1 cut(s) 443
EcoRII CCWGG 1 cut(s) 370
FaqI GGGAC 1 cut(s) 571
FauNDI CATATG 2 cut(s) 130, 535
Fnu4HI GCNGC 2 cut(s) 184, 504
FokI GGATG 1 cut(s) 374
Fsp4HI GCNGC 2 cut(s) 184, 504
FspBI CTAG 1 cut(s) 236
GluI GCNGC 2 cut(s) 184, 504
HaeIII GGCC 2 cut(s) 19, 370
HinfI GANTC 4 cut(s) 5, 72, 217, 480
Hpy166II GTNNAC 2 cut(s) 109, 378
Hpy188I TCNGA 3 cut(s) 83, 216, 272
Hpy188III TCNNGA 3 cut(s) 224, 309, 477
Hpy8I GTNNAC 2 cut(s) 109, 378
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4V TGCA 2 cut(s) 197, 506
HpyF3I CTNAG 2 cut(s) 257, 617
Kzo9I GATC 1 cut(s) 190
LmnI GCTCC 1 cut(s) 323
Lsp1109I GCAGC 2 cut(s) 195, 490
MaeI CTAG 1 cut(s) 236
MalI GATC 1 cut(s) 192
MboI GATC 1 cut(s) 190
MboII GAAGA 5 cut(s) 59, 212, 358, 421, 581
MlsI TGGCCA 1 cut(s) 370
MluCI AATT 8 cut(s) 245, 265, 276, 404, 443, 452, 582, 604
MluNI TGGCCA 1 cut(s) 370
MmeI TCCRAC 2 cut(s) 151, 419
MnlI CCTC 3 cut(s) 122, 266, 402
Mox20I TGGCCA 1 cut(s) 370
MroXI GAANNNNTTC 1 cut(s) 71
MscI TGGCCA 1 cut(s) 370
MseI TTAA 4 cut(s) 242, 438, 603, 622
Msp20I TGGCCA 1 cut(s) 370
MspR9I CCNGG 1 cut(s) 372
MvaI CCWGG 1 cut(s) 372
MvnI CGCG 1 cut(s) 310
NdeI CATATG 2 cut(s) 130, 535
NdeII GATC 1 cut(s) 190
NlaIV GGNNCC 1 cut(s) 290
NruI TCGCGA 1 cut(s) 310
NspV TTCGAA 1 cut(s) 456
PdmI GAANNNNTTC 1 cut(s) 71
PfeI GAWTC 3 cut(s) 72, 217, 480
PkrI GCNGC 2 cut(s) 185, 505
Psp6I CCWGG 1 cut(s) 370
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 1 cut(s) 290
PstI CTGCAG 1 cut(s) 508
RruI TCGCGA 1 cut(s) 310
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
SaqAI TTAA 4 cut(s) 242, 438, 603, 622
SatI GCNGC 2 cut(s) 184, 504
Sau3AI GATC 1 cut(s) 190
ScrFI CCNGG 1 cut(s) 372
SetI ASST 6 cut(s) 80, 114, 171, 232, 258, 318
SfcI CTRYAG 2 cut(s) 381, 504
SfuI TTCGAA 1 cut(s) 456
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
Sse9I AATT 8 cut(s) 245, 265, 276, 404, 443, 452, 582, 604
SspMI CTAG 1 cut(s) 236
StyD4I CCNGG 1 cut(s) 370
TaaI ACNGT 1 cut(s) 139
TaqI TCGA 1 cut(s) 456
TasI AATT 8 cut(s) 245, 265, 276, 404, 443, 452, 582, 604
TfiI GAWTC 3 cut(s) 72, 217, 480
Tru1I TTAA 4 cut(s) 242, 438, 603, 622
Tru9I TTAA 4 cut(s) 242, 438, 603, 622
TscAI CASTG 2 cut(s) 165, 265
TseI GCWGC 2 cut(s) 183, 503
TspDTI ATGAA 4 cut(s) 80, 456, 522, 582
TspRI CASTG 2 cut(s) 165, 265
XapI RAATTY 5 cut(s) 245, 265, 443, 452, 582
XmnI GAANNNNTTC 1 cut(s) 71
XspI CTAG 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.