Rw4G009800

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001930
Physical Location & Seq
Reverse (-)
22290134 .. 22293822
3689 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G009800.1

Sequence Viewer

Length: 2730 bp
ATGGTACCTACATATGAGAATGCAAAGACTTCAACAATTCCAGGTAGCTTGACCATGGCAAATTATCAGAAGCATTTCCTCCGGTTCATCTTCATTGTTATGTTCATCCCTGGGATCGAGCCTTCAACGACGAAAGAGGTGATACGAGTACAAGTCGGAGTTGTTCTTAACTTAAAGTCCTCAGTGGGAGCTATGGCAGAGAACTGCATGACCATGGCACTTGATGATTTTTATGCTAAGCATGCTCATTATCAAACAAGGCTTTCCCTTCTCACCAGCGATTCAAAGAATGATGTTGTTTCAGCAGCAGTTGAAGCACAGCAATTGATAAACATCGAAAGAGTGAGTGTCATTATCGGACCTCAAAGTTCAGCAGAAGCAAAGTTTGTGATAGAGCTGGGAAGAAGGAATCATGTTCCGATTATTTCGTTTTCTGCCACTAGTCCATCTCTTTCTCCATCTAATAGCTCATTTTTCATTCGGACGGCTTTTAGTGACTCTGCTCAAGTAGAAGCCATAGCTGCCATTGTCGGAGCTTATAGTTGGTTGGAAGTTGTTCTCATCTATGAAGACACGGAGTATGGAAATAGCTTAATTCCACATTTCGTAGACGCTTTCCAGAAAGTTGGAGCTCGAGTACCTCATAGAAGTGTCATTCCTCCTCATTATAATGACAGAGAAATTTTAAAGGAGCTTAACCACTTGAAATCAACTCGAGCAAGAATATTTTTGGTCCATATGACTGCTTTCCTCGGGTCCAAATTGTTTTTATTGGCAAATAAGGCAGGGATGATGACCGAAGGGTATGGATGGATTGTTACGGAAGGGTTATCGACTTTATTAGATCCTGTGGGTTCAAGAACCATGGACTCAATGCAAGGTGTAGTGGGAGTAAGGCCATATATACCAATGTCAAAAGAACTTGAAGACTTGGTGTTGCGAATGAAAAGATCAAGCAAGATTACTGGTGTAAACCTCTTTGGGTTGTGGGCATATGATACAGTTTGGGCATTAGCAATGGCAGTGGAGAAGGTTGGAATAGCAAGCAGAGTTGATCCTGCAAGTTTGGGAACCGGTCAGAATCTTCTTGAAGCTATCCTAGATTTAAAATTTGAAAACCTCAGTGGGAATTTCCGGTTGGTTAAGGGACAGTTGGAAGCTTCAACCTTTGAGGTATTTAATGTGATTGGCGGAAAAGAAAGAATAATTGGATATTGGAACCAAAAGAAAGGACTTTCCCGAAAGCTGAATGGAGCAGCAGACTTGGATAAAAGGAAGACGCTCGAGAAACCAATCTGGCCAGGGTACACTACAGACATCCCTCCAACAAAGAAATTGAAGATTGGAGTTCCCTTGAAACAGGGTTTTAATGAATTCCTAAAATTCGAAAACAACCACCATATATCAGGATTCTCTGCTGATATATTCTTTGCTGCAGTAAAGCAACTGCCATTTTCCCTTTCATATGAGTTTTCTTCCTTCCCGGGGACTTATGATGATCTTCTTTATCAAATTTATCACCAGAAATATGACGCTGTGGTGGGAGACACAACAATTGTCGCTAATCGCTCAATATATGTAGATTTTACGTTGCCTTACTCTGAATCAGGAGTTTCCATGATAGTTTTGACAGAAGACAGTGAGAGCAATAGTATTTGGATTTTCTTGAAGCCACTAAGCTTGGGTCTTTGGTTGACAACCGGCATAGCCTTCATATTAACAGGTTTTGTAATATGGGTTCTTGAGCACCGCGAAAACACTGAGTTCAGAGGTCCTCCACGGCAACAACTGGGCATGATATTCTGGTTCTCCTTTTCAACCCTCGTCTTTGCACACAGAGAGAAAGTGGTGAACAATTGGTCAAGGTTTGTGCTGATTATATGGGTTTTTGTGGTGCTGATCCTAACACAGAGTTACACTGCAAGCTTAGCCTCAATGTTAACAGTACAGAAGTTGCAGCCTGCATTCACTGATATAAATGAGATACAAAGAAATGGTTACAAAGTAGGATATCAAAATGGTTCCTTTATTAAAGGGTTTCTCATAGAACATTTAAAATTTGATGTGACCAAGCTGGTGCCATTGGAAACCATTTCGGATTATCATAACGCACTGTCTAGAGGAAACAAGAATGGAGTTGCTGCCATTTTAGATGAAGTCCCTTACCTCAAGTTGTTTCTTAAAGCGAAATGTTCCAAGTACACCATGGTTGGACCAACCTACAAAACTGATGGATTTGGCTTTGCCTTCCCAATACGATCCCCGCTGGTATCCTACATGTCGAGGGCAATCTTGAACGTGACTCAAGATAAAAATACTTACTTTGATTACTTTGATAACAAACCAGCCTGTGAGGATCAAAGTGCCAAAATTTCTTCAGAGGGTCCAAGTCTGGGTGTCTACAGCTTCGGAGGCCTCTTCATCATCGCTGGTGTGGCCTCCATGGTTGCTCTTTTGATGTACATGTATCACTTCCTTTGCTCTCATTGGCCAGCTTTGCGAACTGAGAGCACATTTTGGGAGAAATTGGTTGAAGTGGCAAAGCATTTTGACAAGAAAGATCTCACTTCACATCAGTTTAAGCGAAGAGAATCAAGAGTACATGCTTTAGACACTCCTGATGAAGGTCTAGCAGCTGCACCTGGTGCTAATGATATGCAAAACAACTCAGCAGGAACTCACATAACAGTGGAAAACAATCTTGGACATGATGAAAATGAAAATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

909

Amino Acids

101.76

Weight (kDa)

7.36

Isoelectric Point (pI)

33.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 52 - 377 1.1e-23 Periplasmic binding protein
ANF_receptor PF01094 67 - 397 1.5e-76 Receptor family ligand binding region
SBP_bac_3 PF00497 462 - 610 5.3e-08 Bacterial extracellular solute-binding proteins, family 3
Lig_chan-Glu_bd PF10613 462 - 542 6.6e-06 Ligated ion channel L-glutamate- and glycine-binding site
Lig_chan PF00060 561 - 813 7.6e-37 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 669
Acc65I GGTACC 1 cut(s) 4
AccB1I GGYRCC 2 cut(s) 4, 2077
AccI GTMKAC 2 cut(s) 609, 2400
AccII CGCG 1 cut(s) 1752
AciI CCGC 3 cut(s) 1191, 1750, 2264
AclWI GGATC 6 cut(s) 122, 839, 1049, 1894, 2253, 2364
AcoI YGGCCR 2 cut(s) 1298, 2489
AcsI RAATTY 8 cut(s) 681, 1109, 1129, 1373, 1382, 1512, 2057, 2370
AcuI CTGAAG 1 cut(s) 2361
AdeI CACNNNGTG 1 cut(s) 2645
AfaI GTAC 8 cut(s) 6, 150, 639, 1307, 1947, 2201, 2462, 2601
AfiI CCNNNNNNNGG 3 cut(s) 1485, 2393, 2624
AflIII ACRYGT 2 cut(s) 2277, 2463
AgeI ACCGGT 1 cut(s) 1073
AhlI ACTAGT 1 cut(s) 440
AjnI CCWGG 4 cut(s) 40, 109, 1300, 2641
AjuI GAANNNNNNNTTGG 6 cut(s) 1121, 1153, 1191, 1223, 2685, 2717
Alw21I GWGCWC 3 cut(s) 634, 1749, 2513
Alw26I GTCTC 1 cut(s) 1539
AlwI GGATC 6 cut(s) 122, 839, 1049, 1894, 2253, 2364
Ama87I CYCGRG 5 cut(s) 633, 714, 752, 1283, 1483
AoxI GGCC 5 cut(s) 896, 1298, 2413, 2436, 2489
ApeKI GCWGC 8 cut(s) 305, 521, 1256, 1433, 1957, 2141, 2633, 2636
ApoI RAATTY 8 cut(s) 681, 1109, 1129, 1373, 1382, 1512, 2057, 2370
AsiGI ACCGGT 1 cut(s) 1073
Asp700I GAANNNNTTC 2 cut(s) 74, 555
Asp718I GGTACC 1 cut(s) 4
AspS9I GGNCC 6 cut(s) 359, 733, 756, 1772, 2213, 2384
AsuC2I CCSGG 2 cut(s) 1484, 1485
AsuHPI GGTGA 4 cut(s) 151, 265, 1511, 1861
AsuII TTCGAA 1 cut(s) 1386
AvaI CYCGRG 5 cut(s) 633, 714, 752, 1283, 1483
AvaII GGWCC 6 cut(s) 359, 733, 756, 1772, 2213, 2384
BalI TGGCCA 2 cut(s) 1300, 2491
BanI GGYRCC 2 cut(s) 4, 2077
BanII GRGCYC 1 cut(s) 634
BbsI GAAGAC 4 cut(s) 576, 933, 1283, 1641
Bbv12I GWGCWC 3 cut(s) 634, 1749, 2513
BbvI GCAGC 8 cut(s) 317, 508, 1268, 1420, 1969, 2128, 2623, 2645
BccI CCATC 4 cut(s) 454, 466, 804, 2225
BceAI ACGGC 2 cut(s) 501, 1796
BciT130I CCWGG 4 cut(s) 42, 111, 1302, 2643
BciVI GTATCC 1 cut(s) 2281
BcnI CCSGG 2 cut(s) 1484, 1485
BcoDI GTCTC 1 cut(s) 1539
BcuI ACTAGT 1 cut(s) 440
BfaI CTAG 4 cut(s) 441, 1100, 2118, 2630
BfmI CTRYAG 3 cut(s) 1311, 1434, 2401
BfuI GTATCC 1 cut(s) 2281
BglII AGATCT 1 cut(s) 2560
BisI GCNGC 8 cut(s) 306, 522, 1257, 1434, 1958, 2142, 2634, 2637
BlpI GCTNAGC 2 cut(s) 237, 1927
BlsI GCNGC 8 cut(s) 307, 523, 1258, 1435, 1959, 2143, 2635, 2638
Bme1390I CCNGG 6 cut(s) 42, 111, 1302, 1484, 1485, 2643
Bme18I GGWCC 6 cut(s) 359, 733, 756, 1772, 2213, 2384
BmeT110I CYCGRG 5 cut(s) 633, 714, 752, 1283, 1483
BmgT120I GGNCC 6 cut(s) 359, 733, 756, 1772, 2213, 2384
BmiI GGNNCC 7 cut(s) 6, 757, 1072, 1220, 2023, 2079, 2385
BmrFI CCNGG 6 cut(s) 42, 111, 1302, 1484, 1485, 2643
BmrI ACTGGG 1 cut(s) 1799
BmuI ACTGGG 1 cut(s) 1799
BpiI GAAGAC 4 cut(s) 576, 933, 1283, 1641
Bpu1102I GCTNAGC 2 cut(s) 237, 1927
Bpu14I TTCGAA 1 cut(s) 1386
BpuEI CTTGAG 4 cut(s) 489, 1763, 2153, 2289
BpuMI CCSGG 2 cut(s) 1484, 1485
BsaBI GATNNNNATC 1 cut(s) 332
BsaWI WCCGGW 3 cut(s) 81, 1073, 1134
BsaXI ACNNNNNCTCC 2 cut(s) 621, 651
Bsc4I CCNNNNNNNGG 3 cut(s) 1485, 2393, 2624
Bse118I RCCGGY 2 cut(s) 1073, 1700
Bse1I ACTGG 2 cut(s) 970, 1794
Bse3DI GCAATG 1 cut(s) 1023
Bse8I GATNNNNATC 1 cut(s) 332
BseBI CCWGG 4 cut(s) 42, 111, 1302, 2643
BseGI GGATG 4 cut(s) 105, 795, 815, 1317
BseJI GATNNNNATC 1 cut(s) 332
BseLI CCNNNNNNNGG 3 cut(s) 1485, 2393, 2624
BseMI GCAATG 1 cut(s) 1023
BseMII CTCAG 5 cut(s) 195, 1135, 1752, 2496, 2682
BseNI ACTGG 2 cut(s) 970, 1794
BseRI GAGGAG 1 cut(s) 651
BseXI GCAGC 8 cut(s) 317, 508, 1268, 1420, 1969, 2128, 2623, 2645
BseYI CCCAGC 1 cut(s) 397
BsgI GTGCAG 1 cut(s) 2622
Bsh1236I CGCG 1 cut(s) 1752
BshFI GGCC 5 cut(s) 898, 1300, 2415, 2438, 2491
BshNI GGYRCC 2 cut(s) 4, 2077
BshTI ACCGGT 1 cut(s) 1073
BsiHKAI GWGCWC 3 cut(s) 634, 1749, 2513
BsiHKCI CYCGRG 5 cut(s) 633, 714, 752, 1283, 1483
BsiSI CCGG 5 cut(s) 82, 1074, 1135, 1484, 1701
BslFI GGGAC 3 cut(s) 1161, 1501, 2144
BslI CCNNNNNNNGG 3 cut(s) 1485, 2393, 2624
BsmAI GTCTC 1 cut(s) 1539
BsmFI GGGAC 3 cut(s) 1161, 1501, 2144
BsmI GAATGC 2 cut(s) 25, 1964
BsnI GGCC 5 cut(s) 898, 1300, 2415, 2438, 2491
BsoBI CYCGRG 5 cut(s) 633, 714, 752, 1283, 1483
Bsp119I TTCGAA 1 cut(s) 1386
Bsp1286I GDGCHC 3 cut(s) 634, 1749, 2513
Bsp1407I TGTACA 1 cut(s) 2460
Bsp143I GATC 9 cut(s) 114, 844, 950, 1054, 1498, 1899, 2258, 2356, 2560
Bsp1720I GCTNAGC 2 cut(s) 237, 1927
Bsp19I CCATGG 5 cut(s) 54, 213, 864, 2205, 2442
BspACI CCGC 3 cut(s) 1191, 1750, 2264
BspANI GGCC 5 cut(s) 898, 1300, 2415, 2438, 2491
BspCNI CTCAG 5 cut(s) 194, 1134, 1753, 2497, 2681
BspFNI CGCG 1 cut(s) 1752
BspLI GGNNCC 7 cut(s) 6, 757, 1072, 1220, 2023, 2079, 2385
BspMAI CTGCAG 1 cut(s) 1438
BspPI GGATC 6 cut(s) 122, 839, 1049, 1894, 2253, 2364
BspT104I TTCGAA 1 cut(s) 1386
BspT107I GGYRCC 2 cut(s) 4, 2077
BsrDI GCAATG 1 cut(s) 1023
BsrFI RCCGGY 2 cut(s) 1073, 1700
BsrGI TGTACA 1 cut(s) 2460
BsrI ACTGG 2 cut(s) 970, 1794
BssAI RCCGGY 2 cut(s) 1073, 1700
BssMI GATC 9 cut(s) 114, 844, 950, 1054, 1498, 1899, 2258, 2356, 2560
BssT1I CCWWGG 5 cut(s) 54, 213, 864, 2205, 2442
Bst2UI CCWGG 4 cut(s) 42, 111, 1302, 2643
Bst4CI ACNGT 6 cut(s) 1003, 1152, 1640, 1945, 2115, 2689
Bst6I CTCTTC 2 cut(s) 2423, 2581
BstAPI GCANNNNNTGC 1 cut(s) 2645
BstAUI TGTACA 1 cut(s) 2460
BstBI TTCGAA 1 cut(s) 1386
BstC8I GCNNGC 5 cut(s) 243, 1045, 1924, 1962, 2493
BstDEI CTNAG 8 cut(s) 181, 237, 1121, 1676, 1761, 1927, 2505, 2668
BstDSI CCRYGG 6 cut(s) 54, 213, 864, 1778, 2205, 2442
BstENI CCTNNNNNAGG 1 cut(s) 2622
BstF5I GGATG 4 cut(s) 105, 795, 815, 1317
BstFNI CGCG 1 cut(s) 1752
BstKTI GATC 9 cut(s) 117, 847, 953, 1057, 1501, 1902, 2261, 2359, 2563
BstMAI GTCTC 1 cut(s) 1539
BstMBI GATC 9 cut(s) 114, 844, 950, 1054, 1498, 1899, 2258, 2356, 2560
BstMWI GCNNNNNNNGC 9 cut(s) 242, 314, 521, 782, 1928, 2412, 2435, 2497, 2645
BstNI CCWGG 4 cut(s) 42, 111, 1302, 2643
BstNSI RCATGY 4 cut(s) 245, 2281, 2467, 2606
BstSCI CCNGG 6 cut(s) 40, 109, 1300, 1482, 1483, 2641
BstSFI CTRYAG 3 cut(s) 1311, 1434, 2401
BstUI CGCG 1 cut(s) 1752
BstV1I GCAGC 8 cut(s) 317, 508, 1268, 1420, 1969, 2128, 2623, 2645
BstV2I GAAGAC 4 cut(s) 576, 933, 1283, 1641
BstX2I RGATCY 2 cut(s) 844, 2560
BstXI CCANNNNNNTGG 1 cut(s) 626
BstYI RGATCY 2 cut(s) 844, 2560
BsuI GTATCC 1 cut(s) 2281
BsuRI GGCC 5 cut(s) 898, 1300, 2415, 2438, 2491
BtgI CCRYGG 6 cut(s) 54, 213, 864, 1778, 2205, 2442
BtgZI GCGATG 1 cut(s) 2410
BtsCI GGATG 4 cut(s) 105, 795, 815, 1317
BtsI GCAGTG 2 cut(s) 1029, 1917
BtsIMutI CAGTG 9 cut(s) 189, 1029, 1129, 1645, 1758, 1917, 1968, 2111, 2694
Cac8I GCNNGC 5 cut(s) 243, 1045, 1924, 1962, 2493
Cfr10I RCCGGY 2 cut(s) 1073, 1700
Cfr13I GGNCC 6 cut(s) 359, 733, 756, 1772, 2213, 2384
Cfr9I CCCGGG 1 cut(s) 1483
CseI GACGC 3 cut(s) 620, 1288, 1541
CsiI ACCWGGT 1 cut(s) 2641
Csp6I GTAC 8 cut(s) 5, 149, 638, 1306, 1946, 2200, 2461, 2600
CspAI ACCGGT 1 cut(s) 1073
CspCI CAANNNNNGTGG 4 cut(s) 1005, 1040, 1662, 1697
CviQI GTAC 8 cut(s) 5, 149, 638, 1306, 1946, 2200, 2461, 2600
DdeI CTNAG 8 cut(s) 181, 237, 1121, 1676, 1761, 1927, 2505, 2668
DpnI GATC 9 cut(s) 116, 846, 952, 1056, 1500, 1901, 2260, 2358, 2562
DpnII GATC 9 cut(s) 114, 844, 950, 1054, 1498, 1899, 2258, 2356, 2560
DraI TTTAAA 3 cut(s) 687, 1107, 2055
DraIII CACNNNGTG 1 cut(s) 2645
EaeI YGGCCR 2 cut(s) 1298, 2489
Eam1104I CTCTTC 2 cut(s) 2423, 2581
EarI CTCTTC 2 cut(s) 2423, 2581
EciI GGCGGA 1 cut(s) 1206
Ecl136II GAGCTC 1 cut(s) 632
Eco130I CCWWGG 5 cut(s) 54, 213, 864, 2205, 2442
Eco147I AGGCCT 1 cut(s) 2415
Eco24I GRGCYC 1 cut(s) 634
Eco32I GATATC 1 cut(s) 2012
Eco47I GGWCC 6 cut(s) 359, 733, 756, 1772, 2213, 2384
Eco53kI GAGCTC 1 cut(s) 632
Eco57I CTGAAG 1 cut(s) 2361
Eco88I CYCGRG 5 cut(s) 633, 714, 752, 1283, 1483
EcoICRI GAGCTC 1 cut(s) 632
EcoNI CCTNNNNNAGG 1 cut(s) 2622
EcoO109I RGGNCCY 1 cut(s) 1772
EcoRI GAATTC 1 cut(s) 1373
EcoRII CCWGG 4 cut(s) 40, 109, 1300, 2641
EcoRV GATATC 1 cut(s) 2012
EcoT14I CCWWGG 5 cut(s) 54, 213, 864, 2205, 2442
EcoT38I GRGCYC 1 cut(s) 634
ErhI CCWWGG 5 cut(s) 54, 213, 864, 2205, 2442
FaqI GGGAC 3 cut(s) 1161, 1501, 2144
FauI CCCGC 1 cut(s) 2271
FauNDI CATATG 4 cut(s) 13, 738, 994, 1465
FblI GTMKAC 2 cut(s) 609, 2400
Fnu4HI GCNGC 8 cut(s) 306, 522, 1257, 1434, 1958, 2142, 2634, 2637
FokI GGATG 4 cut(s) 92, 802, 822, 1304
FriOI GRGCYC 1 cut(s) 634
Fsp4HI GCNGC 8 cut(s) 306, 522, 1257, 1434, 1958, 2142, 2634, 2637
FspBI CTAG 4 cut(s) 441, 1100, 2118, 2630
GluI GCNGC 8 cut(s) 306, 522, 1257, 1434, 1958, 2142, 2634, 2637
GsaI CCCAGC 1 cut(s) 401
HaeIII GGCC 5 cut(s) 898, 1300, 2415, 2438, 2491
HapII CCGG 5 cut(s) 82, 1074, 1135, 1484, 1701
HgaI GACGC 3 cut(s) 620, 1288, 1541
HincII GTYRAC 2 cut(s) 1695, 1941
HindII GTYRAC 2 cut(s) 1695, 1941
HindIII AAGCTT 3 cut(s) 1158, 1678, 1924
HinfI GANTC 9 cut(s) 281, 409, 497, 869, 1081, 1410, 1604, 2302, 2591
HpaI GTTAAC 1 cut(s) 1941
HpaII CCGG 5 cut(s) 82, 1074, 1135, 1484, 1701
HphI GGTGA 4 cut(s) 151, 265, 1511, 1861
Hpy166II GTNNAC 8 cut(s) 610, 973, 1308, 1695, 1852, 1941, 2202, 2401
Hpy8I GTNNAC 8 cut(s) 610, 973, 1308, 1695, 1852, 1941, 2202, 2401
Hpy99I CGWCG 1 cut(s) 133
HpyCH4III ACNGT 6 cut(s) 1003, 1152, 1640, 1945, 2115, 2689
HpyCH4IV ACGT 2 cut(s) 1589, 2298
HpyF10VI GCNNNNNNNGC 9 cut(s) 242, 314, 521, 782, 1928, 2412, 2435, 2497, 2645
HpyF3I CTNAG 8 cut(s) 181, 237, 1121, 1676, 1761, 1927, 2505, 2668
HpySE526I ACGT 2 cut(s) 1589, 2298
KpnI GGTACC 1 cut(s) 8
KspAI GTTAAC 1 cut(s) 1941
Kzo9I GATC 9 cut(s) 114, 844, 950, 1054, 1498, 1899, 2258, 2356, 2560
LmnI GCTCC 5 cut(s) 188, 533, 629, 691, 1253
Lsp1109I GCAGC 8 cut(s) 317, 508, 1268, 1420, 1969, 2128, 2623, 2645
MabI ACCWGGT 1 cut(s) 2641
MaeI CTAG 4 cut(s) 441, 1100, 2118, 2630
MaeII ACGT 2 cut(s) 1589, 2298
MaeIII GTNAC 6 cut(s) 494, 817, 1913, 1997, 2065, 2299
MalI GATC 9 cut(s) 116, 846, 952, 1056, 1500, 1901, 2260, 2358, 2562
MboI GATC 9 cut(s) 114, 844, 950, 1054, 1498, 1899, 2258, 2356, 2560
MfeI CAATTG 3 cut(s) 323, 1554, 1855
MflI RGATCY 2 cut(s) 844, 2560
MhlI GDGCHC 3 cut(s) 634, 1749, 2513
MlsI TGGCCA 2 cut(s) 1300, 2491
MluNI TGGCCA 2 cut(s) 1300, 2491
MlyI GAGTC 3 cut(s) 491, 863, 2296
MmeI TCCRAC 8 cut(s) 136, 511, 528, 607, 1015, 1134, 1349, 2191
Mox20I TGGCCA 2 cut(s) 1300, 2491
MroXI GAANNNNTTC 2 cut(s) 74, 555
MscI TGGCCA 2 cut(s) 1300, 2491
MslI CAYNNNNRTG 5 cut(s) 98, 212, 648, 669, 2687
Msp20I TGGCCA 2 cut(s) 1300, 2491
MspA1I CMGCKG 2 cut(s) 2266, 2636
MspI CCGG 5 cut(s) 82, 1074, 1135, 1484, 1701
MspR9I CCNGG 6 cut(s) 42, 111, 1302, 1484, 1485, 2643
MunI CAATTG 3 cut(s) 323, 1554, 1855
Mva1269I GAATGC 2 cut(s) 25, 1964
MvaI CCWGG 4 cut(s) 42, 111, 1302, 2643
MvnI CGCG 1 cut(s) 1752
MwoI GCNNNNNNNGC 9 cut(s) 242, 314, 521, 782, 1928, 2412, 2435, 2497, 2645
NciI CCSGG 2 cut(s) 1484, 1485
NcoI CCATGG 5 cut(s) 54, 213, 864, 2205, 2442
NdeI CATATG 4 cut(s) 13, 738, 994, 1465
NdeII GATC 9 cut(s) 114, 844, 950, 1054, 1498, 1899, 2258, 2356, 2560
NlaIV GGNNCC 7 cut(s) 6, 757, 1072, 1220, 2023, 2079, 2385
NmuCI GTSAC 3 cut(s) 494, 2065, 2299
NspI RCATGY 4 cut(s) 245, 2281, 2467, 2606
NspV TTCGAA 1 cut(s) 1386
PaeI GCATGC 1 cut(s) 245
PaeR7I CTCGAG 3 cut(s) 633, 714, 1283
PasI CCCWGGG 1 cut(s) 110
PceI AGGCCT 1 cut(s) 2415
PciI ACATGT 2 cut(s) 2277, 2463
PctI GAATGC 2 cut(s) 25, 1964
PdmI GAANNNNTTC 2 cut(s) 74, 555
PfeI GAWTC 6 cut(s) 281, 409, 1081, 1410, 1604, 2591
PinAI ACCGGT 1 cut(s) 1073
PkrI GCNGC 8 cut(s) 307, 523, 1258, 1435, 1959, 2143, 2635, 2638
PleI GAGTC 3 cut(s) 491, 863, 2296
PpsI GAGTC 3 cut(s) 491, 863, 2296
PpuMI RGGWCCY 1 cut(s) 1772
PscI ACATGT 2 cut(s) 2277, 2463
PsiI TTATAA 1 cut(s) 669
Psp124BI GAGCTC 1 cut(s) 634
Psp5II RGGWCCY 1 cut(s) 1772
Psp6I CCWGG 4 cut(s) 40, 109, 1300, 2641
PspFI CCCAGC 1 cut(s) 397
PspGI CCWGG 4 cut(s) 40, 109, 1300, 2641
PspN4I GGNNCC 7 cut(s) 6, 757, 1072, 1220, 2023, 2079, 2385
PspPI GGNCC 6 cut(s) 359, 733, 756, 1772, 2213, 2384
PspPPI RGGWCCY 1 cut(s) 1772
PspXI VCTCGAGB 2 cut(s) 633, 714
PsrI GAACNNNNNNTAC 2 cut(s) 1722, 1754
PstI CTGCAG 1 cut(s) 1438
PsuI RGATCY 2 cut(s) 844, 2560
PvuII CAGCTG 1 cut(s) 2636
RsaI GTAC 8 cut(s) 6, 150, 639, 1307, 1947, 2201, 2462, 2601
RsaNI GTAC 8 cut(s) 5, 149, 638, 1306, 1946, 2200, 2461, 2600
RseI CAYNNNNRTG 5 cut(s) 98, 212, 648, 669, 2687
SacI GAGCTC 1 cut(s) 634
SatI GCNGC 8 cut(s) 306, 522, 1257, 1434, 1958, 2142, 2634, 2637
Sau3AI GATC 9 cut(s) 114, 844, 950, 1054, 1498, 1899, 2258, 2356, 2560
Sau96I GGNCC 6 cut(s) 359, 733, 756, 1772, 2213, 2384
SchI GAGTC 3 cut(s) 491, 863, 2296
ScrFI CCNGG 6 cut(s) 42, 111, 1302, 1484, 1485, 2643
SduI GDGCHC 3 cut(s) 634, 1749, 2513
SexAI ACCWGGT 1 cut(s) 2641
SfcI CTRYAG 3 cut(s) 1311, 1434, 2401
Sfr274I CTCGAG 3 cut(s) 633, 714, 1283
SfuI TTCGAA 1 cut(s) 1386
SinI GGWCC 6 cut(s) 359, 733, 756, 1772, 2213, 2384
SlaI CTCGAG 3 cut(s) 633, 714, 1283
SmaI CCCGGG 1 cut(s) 1485
SmiMI CAYNNNNRTG 5 cut(s) 98, 212, 648, 669, 2687
SmlI CTYRAG 7 cut(s) 504, 633, 714, 1283, 1742, 2168, 2304
SmoI CTYRAG 7 cut(s) 504, 633, 714, 1283, 1742, 2168, 2304
SpeI ACTAGT 1 cut(s) 440
SphI GCATGC 1 cut(s) 245
SseBI AGGCCT 1 cut(s) 2415
SsiI CCGC 3 cut(s) 1191, 1750, 2264
SspI AATATT 1 cut(s) 726
SspMI CTAG 4 cut(s) 441, 1100, 2118, 2630
SstI GAGCTC 1 cut(s) 634
StuI AGGCCT 1 cut(s) 2415
StyD4I CCNGG 6 cut(s) 40, 109, 1300, 1482, 1483, 2641
StyI CCWWGG 5 cut(s) 54, 213, 864, 2205, 2442
TaaI ACNGT 6 cut(s) 1003, 1152, 1640, 1945, 2115, 2689
TaiI ACGT 2 cut(s) 1592, 2301
TaqI TCGA 8 cut(s) 117, 336, 634, 715, 833, 1284, 1386, 2282
TaqII GACCGA 1 cut(s) 812
TatI WGTACW 5 cut(s) 148, 1945, 2199, 2460, 2599
TfiI GAWTC 6 cut(s) 281, 409, 1081, 1410, 1604, 2591
TscAI CASTG 9 cut(s) 189, 1029, 1129, 1645, 1765, 1924, 1975, 2118, 2694
TseFI GTSAC 3 cut(s) 494, 2065, 2299
TseI GCWGC 8 cut(s) 305, 521, 1256, 1433, 1957, 2141, 2633, 2636
Tsp45I GTSAC 3 cut(s) 494, 2065, 2299
TspGWI ACGGA 2 cut(s) 590, 836
TspMI CCCGGG 1 cut(s) 1483
TspRI CASTG 9 cut(s) 189, 1029, 1129, 1645, 1765, 1924, 1975, 2118, 2694
VpaK11BI GGWCC 6 cut(s) 359, 733, 756, 1772, 2213, 2384
XagI CCTNNNNNAGG 1 cut(s) 2622
XapI RAATTY 8 cut(s) 681, 1109, 1129, 1373, 1382, 1512, 2057, 2370
XbaI TCTAGA 1 cut(s) 2117
XceI RCATGY 4 cut(s) 245, 2281, 2467, 2606
XcmI CCANNNNNNNNNTGG 1 cut(s) 2203
XhoI CTCGAG 3 cut(s) 633, 714, 1283
XmaI CCCGGG 1 cut(s) 1483
XmiI GTMKAC 2 cut(s) 609, 2400
XmnI GAANNNNTTC 2 cut(s) 74, 555
XspI CTAG 4 cut(s) 441, 1100, 2118, 2630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.