Rw4G009930

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001930
Physical Location & Seq
Forward (+)
22622824 .. 22626052
3229 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G009930.1

Sequence Viewer

Length: 2721 bp
ATGGCAAACCAGAAGCATTTCCTCTGGTTCATCTTCATTGTTTTGTTCATCCCTGGGATCGAGCCTTCAACGACAAAAGAGGTGATACGAGTACCAGTAGGAGTTGTTCTTAACTTAAATTCCTCAGTGGGAGCTATGGCAGAGAACTGCATGACCATGGCACTTGATGATTTCTATGCTAAGCATGCTCATTATCAAACAAGGCTTTTCTTTCTCACCAGGGATTCAAAGAATGATGTTGTTTCAGCAGCATTTGAAGCACAGCACTTGATAAAAATTGAAAATGTGAGTGCCATTATCGGACCTCAAAGTTCAGCAGAAGCAAAGTTTGTGATAGAGCTGGGAAGAAGGAATCATGTTCCGATTATTTCGTTTTCTTCCACTAGTCCATCTCTTTCTCCATCTAATAGCTCATTTTTCATTCGGACGGCTTTTAGTGACTCTGCTCATGTAGAAGCCATAGCTGCCATTGTCGGAGCTTATAGTTGGTTGGAAGTTGTTCTCATCTATGAAGACACGGAGTATGGAAATAGCTTAATTCCACATTTGGTAGACGCTTTCCAGAAAGTTGGAGCTCGAGTACCTCATAGAAGTATCATTCCTCCTCATTATAATGACAGAGAAATTTTAAAGGAGCTTAAACACTTGAAATCAACTCGAGCAAGAATATTTTTGGTCCATATGACTGCTTTCCTCGGGTCCAAATTGTTTTTATTGGCAAATAAGGCAGGGATGATGACCGAAGGGTATGGATGGATTGTTACGGAAGGGTTATCGACTTTATTAGATCCTGTGGGTTCAAGAACCATGGACTCAATGCAAGGTGTAGTGGGAGTAAGGCCATATATACCAATGTCAAAAGAACTTGAAGACTTGGTGTTGCGAATGAAAAGATCAAGCAAGATTACTGGTGTAAACCTCTTTGGGTTGTGGGCATATGATACAGTTTGGGCATTAGCAATGGCAGTGGAGAAGGTTGGAATAGCAAGCAGAGTTGATCCTGCAAGTTTGGGAACTGGTCAGAATCTTCTTGAAGCTATCCTAGATTTAAAATTTGAAAACCTCAGTGGGAATTTCCGGTTGGTTAAGGGACAGTTGGAAGCTTCAACCTTTGAGGTATTTAATGTGATTGGCGGAAAAGAAAGAATAATTGGATATTGGAACCAAAAGAAAGGACTTTCCCGAAAGCTGAATGGAGCAGCAGACTTGGATAAAAGGAAGACACTCGAGAAACCAATCTGGCCAGGGTACACTACAGACATCCCTCCAACAAAGAAATTGAAGATTGGAGTTCCCTTGAAACAGGGTTTTAATGAATTCCTAAAATTCGAAAACAACCACCATATATCAGGATTCTCTGCTGATATATTCTTTGCTGCAGTAAAGCAACTGCCATTTTCCCTTTCATATGAGTTTTCTTCCTTCCTGGGGACTTACGATGATCTTCTTTATCAAATTTATCGCCAGAAATATGACGCTGTGGTGGGAGACACAACAATTGTCGCTAATCGATCAATATATGTAGATTTTACGTTACCTTACTCTGAATCAGGAGTTTCCATGATAGTTTTGACAGAAGACAGTGAGAGCAGTAGTATTTGGATTTTCTTGAAGCCACTAAGCTTGGGTCTTTGGTTGACAACCGGCATAGCCTTCATATTAACAGGTTTTGTAATATGGGTTCTTGAGCACCGCGAAAACACTGAGTTCAGAGGTCCTCCACGGCAACAACTGGGCATGATATTCTGGTTCTCCTTTTCAACCCTCGTCTTTGCACACAGAGAGAAAGTGGTGAACAATTGGTCAAGGTTTGTGCTGATTATATGGGTTTTTGTGGTGCTGATCCTAACACAGAGTTACACTGCAAGCTTAGCCTCAATGTTAACAGTACAGAAGTTGCAGCCTGCATTCACTGATATAAATGAGATACAAAGAAATGGTTACAAAGTAGGATATCAAAATGGTTCCTTTATTAAAGGGTTTCTCATAGAACATTTAAAATTTGATGTGACCAAGCTGGTGCCATTGGAAACCATTTCGGATTATCATAACGCACTGTCTAGAGGAAACAAGAATGGAGTTGCTGCCATTTTAGATGAAGTCCCTTACCTCAAGTTGTTTCTTAAAGCGAAATGTTCCAAGTACACCATGGTTGGACCAACCTACAAAACTGATGGATTTGGCTTTGCCTTCCCAATACGATCCCCGCTGGTATCCTACATGTCGAGGGCAATCTTGAACGTGACTCAAGATAAAAATACTTACTTTGATTACTTTGATAACAAACAAGCCTGTGAGGATCAAAGTGCCAAAATTTCTTCAGAGGGTCCAAGTCTGGGTGTCTACAGCTTCGGAGGCCTCTTCATCATCGCTGGTGTGGCCTCCATGGTTGCTCTTTTGATGTACATGTATCACTTCCTTTGCTCTCATTGGCCAGCTTTGCGAACTGAGAGCACATTTTGGGAGAAATTGGTTGAAGTGGCAAATCATTTTGACAAGAAAGATCTCACTTCACATCAGTTTAAGCGAAGAGAATCAAGAGTACATGCTTTAGACACTCCTGATGAAGGTCTAGCAGCTGCACCTGGTGCTAATGATATGCAAAACAACTCAGCAGGAACTCACATAACAGTGGAAAACAATCTTGGACATGATGAAAATGAAAATCTTTCATCAGGGCATACGGATTCATCTATGCCTGTACTCGACTCATCATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

906

Amino Acids

101.45

Weight (kDa)

6.96

Isoelectric Point (pI)

36.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 41 - 358 1.6e-21 Periplasmic binding protein
ANF_receptor PF01094 48 - 378 3.7e-75 Receptor family ligand binding region
SBP_bac_3 PF00497 443 - 591 6.5e-08 Bacterial extracellular solute-binding proteins, family 3
Lig_chan-Glu_bd PF10613 443 - 523 6.3e-06 Ligated ion channel L-glutamate- and glycine-binding site
Lig_chan PF00060 542 - 794 4.1e-37 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 612
AccB1I GGYRCC 1 cut(s) 2020
AccI GTMKAC 2 cut(s) 552, 2343
AccII CGCG 1 cut(s) 1695
AciI CCGC 3 cut(s) 1134, 1693, 2207
AclWI GGATC 6 cut(s) 65, 782, 992, 1837, 2196, 2307
AcoI YGGCCR 2 cut(s) 1241, 2432
AcsI RAATTY 9 cut(s) 118, 624, 1052, 1072, 1316, 1325, 1455, 2000, 2313
AcuI CTGAAG 1 cut(s) 2304
AdeI CACNNNGTG 1 cut(s) 2588
AfaI GTAC 8 cut(s) 93, 582, 1250, 1890, 2144, 2405, 2544, 2703
AfiI CCNNNNNNNGG 3 cut(s) 1428, 2336, 2567
AflIII ACRYGT 2 cut(s) 2220, 2406
AhlI ACTAGT 1 cut(s) 383
AjnI CCWGG 5 cut(s) 52, 218, 1243, 1425, 2584
AjuI GAANNNNNNNTTGG 6 cut(s) 1064, 1096, 1134, 1166, 2628, 2660
Alw21I GWGCWC 3 cut(s) 577, 1692, 2456
Alw26I GTCTC 1 cut(s) 1482
AlwI GGATC 6 cut(s) 65, 782, 992, 1837, 2196, 2307
Ama87I CYCGRG 4 cut(s) 576, 657, 695, 1226
AoxI GGCC 5 cut(s) 839, 1241, 2356, 2379, 2432
ApeKI GCWGC 8 cut(s) 248, 464, 1199, 1376, 1900, 2084, 2576, 2579
ApoI RAATTY 9 cut(s) 118, 624, 1052, 1072, 1316, 1325, 1455, 2000, 2313
Asp700I GAANNNNTTC 2 cut(s) 17, 498
AspS9I GGNCC 6 cut(s) 302, 676, 699, 1715, 2156, 2327
AsuHPI GGTGA 3 cut(s) 94, 208, 1804
AsuII TTCGAA 1 cut(s) 1329
AvaI CYCGRG 4 cut(s) 576, 657, 695, 1226
AvaII GGWCC 6 cut(s) 302, 676, 699, 1715, 2156, 2327
BalI TGGCCA 2 cut(s) 1243, 2434
BanI GGYRCC 1 cut(s) 2020
BanII GRGCYC 1 cut(s) 577
BbsI GAAGAC 4 cut(s) 519, 876, 1226, 1584
Bbv12I GWGCWC 3 cut(s) 577, 1692, 2456
BbvI GCAGC 8 cut(s) 260, 451, 1211, 1363, 1912, 2071, 2566, 2588
BccI CCATC 4 cut(s) 397, 409, 747, 2168
BceAI ACGGC 2 cut(s) 444, 1739
BciT130I CCWGG 5 cut(s) 54, 220, 1245, 1427, 2586
BciVI GTATCC 1 cut(s) 2224
BcoDI GTCTC 1 cut(s) 1482
BcuI ACTAGT 1 cut(s) 383
BfaI CTAG 4 cut(s) 384, 1043, 2061, 2573
BfmI CTRYAG 3 cut(s) 1254, 1377, 2344
BfuI GTATCC 1 cut(s) 2224
BglII AGATCT 1 cut(s) 2503
BisI GCNGC 8 cut(s) 249, 465, 1200, 1377, 1901, 2085, 2577, 2580
BlpI GCTNAGC 2 cut(s) 180, 1870
BlsI GCNGC 8 cut(s) 250, 466, 1201, 1378, 1902, 2086, 2578, 2581
Bme1390I CCNGG 5 cut(s) 54, 220, 1245, 1427, 2586
Bme18I GGWCC 6 cut(s) 302, 676, 699, 1715, 2156, 2327
BmeT110I CYCGRG 4 cut(s) 576, 657, 695, 1226
BmgT120I GGNCC 6 cut(s) 302, 676, 699, 1715, 2156, 2327
BmiI GGNNCC 5 cut(s) 700, 1163, 1966, 2022, 2328
BmrFI CCNGG 5 cut(s) 54, 220, 1245, 1427, 2586
BmrI ACTGGG 1 cut(s) 1742
BmuI ACTGGG 1 cut(s) 1742
BpiI GAAGAC 4 cut(s) 519, 876, 1226, 1584
Bpu1102I GCTNAGC 2 cut(s) 180, 1870
Bpu14I TTCGAA 1 cut(s) 1329
BpuEI CTTGAG 3 cut(s) 1706, 2096, 2232
Bsa29I ATCGAT 1 cut(s) 1510
BsaWI WCCGGW 1 cut(s) 1077
BsaXI ACNNNNNCTCC 2 cut(s) 564, 594
Bsc4I CCNNNNNNNGG 3 cut(s) 1428, 2336, 2567
Bse118I RCCGGY 1 cut(s) 1643
Bse1I ACTGG 4 cut(s) 95, 913, 1021, 1737
Bse3DI GCAATG 1 cut(s) 966
BseBI CCWGG 5 cut(s) 54, 220, 1245, 1427, 2586
BseCI ATCGAT 1 cut(s) 1510
BseGI GGATG 4 cut(s) 48, 738, 758, 1260
BseLI CCNNNNNNNGG 3 cut(s) 1428, 2336, 2567
BseMI GCAATG 1 cut(s) 966
BseMII CTCAG 5 cut(s) 138, 1078, 1695, 2439, 2625
BseNI ACTGG 4 cut(s) 95, 913, 1021, 1737
BseRI GAGGAG 1 cut(s) 594
BseXI GCAGC 8 cut(s) 260, 451, 1211, 1363, 1912, 2071, 2566, 2588
BseYI CCCAGC 1 cut(s) 340
BsgI GTGCAG 1 cut(s) 2565
Bsh1236I CGCG 1 cut(s) 1695
BshFI GGCC 5 cut(s) 841, 1243, 2358, 2381, 2434
BshNI GGYRCC 1 cut(s) 2020
BshVI ATCGAT 1 cut(s) 1510
BsiHKAI GWGCWC 3 cut(s) 577, 1692, 2456
BsiHKCI CYCGRG 4 cut(s) 576, 657, 695, 1226
BsiSI CCGG 2 cut(s) 1078, 1644
BslFI GGGAC 3 cut(s) 1104, 1444, 2087
BslI CCNNNNNNNGG 3 cut(s) 1428, 2336, 2567
BsmAI GTCTC 1 cut(s) 1482
BsmFI GGGAC 3 cut(s) 1104, 1444, 2087
BsmI GAATGC 1 cut(s) 1907
BsnI GGCC 5 cut(s) 841, 1243, 2358, 2381, 2434
BsoBI CYCGRG 4 cut(s) 576, 657, 695, 1226
Bsp119I TTCGAA 1 cut(s) 1329
Bsp1286I GDGCHC 3 cut(s) 577, 1692, 2456
Bsp1407I TGTACA 1 cut(s) 2403
Bsp1720I GCTNAGC 2 cut(s) 180, 1870
Bsp19I CCATGG 4 cut(s) 156, 807, 2148, 2385
BspACI CCGC 3 cut(s) 1134, 1693, 2207
BspANI GGCC 5 cut(s) 841, 1243, 2358, 2381, 2434
BspCNI CTCAG 5 cut(s) 137, 1077, 1696, 2440, 2624
BspDI ATCGAT 1 cut(s) 1510
BspFNI CGCG 1 cut(s) 1695
BspLI GGNNCC 5 cut(s) 700, 1163, 1966, 2022, 2328
BspMAI CTGCAG 1 cut(s) 1381
BspPI GGATC 6 cut(s) 65, 782, 992, 1837, 2196, 2307
BspT104I TTCGAA 1 cut(s) 1329
BspT107I GGYRCC 1 cut(s) 2020
BsrDI GCAATG 1 cut(s) 966
BsrFI RCCGGY 1 cut(s) 1643
BsrGI TGTACA 1 cut(s) 2403
BsrI ACTGG 4 cut(s) 95, 913, 1021, 1737
BssAI RCCGGY 1 cut(s) 1643
BssT1I CCWWGG 4 cut(s) 156, 807, 2148, 2385
Bst2UI CCWGG 5 cut(s) 54, 220, 1245, 1427, 2586
Bst4CI ACNGT 6 cut(s) 946, 1095, 1583, 1888, 2058, 2632
Bst6I CTCTTC 2 cut(s) 2366, 2524
BstAPI GCANNNNNTGC 1 cut(s) 2588
BstAUI TGTACA 1 cut(s) 2403
BstBI TTCGAA 1 cut(s) 1329
BstC8I GCNNGC 5 cut(s) 186, 988, 1867, 1905, 2436
BstDEI CTNAG 8 cut(s) 124, 180, 1064, 1619, 1704, 1870, 2448, 2611
BstDSI CCRYGG 5 cut(s) 156, 807, 1721, 2148, 2385
BstENI CCTNNNNNAGG 1 cut(s) 2565
BstF5I GGATG 4 cut(s) 48, 738, 758, 1260
BstFNI CGCG 1 cut(s) 1695
BstMAI GTCTC 1 cut(s) 1482
BstMWI GCNNNNNNNGC 9 cut(s) 185, 257, 464, 725, 1871, 2355, 2378, 2440, 2588
BstNI CCWGG 5 cut(s) 54, 220, 1245, 1427, 2586
BstNSI RCATGY 4 cut(s) 188, 2224, 2410, 2549
BstSCI CCNGG 5 cut(s) 52, 218, 1243, 1425, 2584
BstSFI CTRYAG 3 cut(s) 1254, 1377, 2344
BstUI CGCG 1 cut(s) 1695
BstV1I GCAGC 8 cut(s) 260, 451, 1211, 1363, 1912, 2071, 2566, 2588
BstV2I GAAGAC 4 cut(s) 519, 876, 1226, 1584
BstX2I RGATCY 2 cut(s) 787, 2503
BstXI CCANNNNNNTGG 1 cut(s) 569
BstYI RGATCY 2 cut(s) 787, 2503
Bsu15I ATCGAT 1 cut(s) 1510
BsuI GTATCC 1 cut(s) 2224
BsuRI GGCC 5 cut(s) 841, 1243, 2358, 2381, 2434
BsuTUI ATCGAT 1 cut(s) 1510
BtgI CCRYGG 5 cut(s) 156, 807, 1721, 2148, 2385
BtgZI GCGATG 1 cut(s) 2353
BtsCI GGATG 4 cut(s) 48, 738, 758, 1260
BtsI GCAGTG 2 cut(s) 972, 1860
BtsIMutI CAGTG 9 cut(s) 132, 972, 1072, 1588, 1701, 1860, 1911, 2054, 2637
Cac8I GCNNGC 5 cut(s) 186, 988, 1867, 1905, 2436
Cfr10I RCCGGY 1 cut(s) 1643
Cfr13I GGNCC 6 cut(s) 302, 676, 699, 1715, 2156, 2327
ClaI ATCGAT 1 cut(s) 1510
CseI GACGC 2 cut(s) 563, 1484
CsiI ACCWGGT 1 cut(s) 2584
Csp6I GTAC 8 cut(s) 92, 581, 1249, 1889, 2143, 2404, 2543, 2702
CspCI CAANNNNNGTGG 4 cut(s) 948, 983, 1605, 1640
CviQI GTAC 8 cut(s) 92, 581, 1249, 1889, 2143, 2404, 2543, 2702
DdeI CTNAG 8 cut(s) 124, 180, 1064, 1619, 1704, 1870, 2448, 2611
DraI TTTAAA 3 cut(s) 630, 1050, 1998
DraIII CACNNNGTG 1 cut(s) 2588
EaeI YGGCCR 2 cut(s) 1241, 2432
Eam1104I CTCTTC 2 cut(s) 2366, 2524
EarI CTCTTC 2 cut(s) 2366, 2524
EciI GGCGGA 1 cut(s) 1149
Ecl136II GAGCTC 1 cut(s) 575
Eco130I CCWWGG 4 cut(s) 156, 807, 2148, 2385
Eco147I AGGCCT 1 cut(s) 2358
Eco24I GRGCYC 1 cut(s) 577
Eco32I GATATC 1 cut(s) 1955
Eco47I GGWCC 6 cut(s) 302, 676, 699, 1715, 2156, 2327
Eco53kI GAGCTC 1 cut(s) 575
Eco57I CTGAAG 1 cut(s) 2304
Eco88I CYCGRG 4 cut(s) 576, 657, 695, 1226
EcoICRI GAGCTC 1 cut(s) 575
EcoNI CCTNNNNNAGG 1 cut(s) 2565
EcoO109I RGGNCCY 1 cut(s) 1715
EcoRI GAATTC 1 cut(s) 1316
EcoRII CCWGG 5 cut(s) 52, 218, 1243, 1425, 2584
EcoRV GATATC 1 cut(s) 1955
EcoT14I CCWWGG 4 cut(s) 156, 807, 2148, 2385
EcoT38I GRGCYC 1 cut(s) 577
ErhI CCWWGG 4 cut(s) 156, 807, 2148, 2385
FaqI GGGAC 3 cut(s) 1104, 1444, 2087
FauI CCCGC 1 cut(s) 2214
FauNDI CATATG 3 cut(s) 681, 937, 1408
FblI GTMKAC 2 cut(s) 552, 2343
Fnu4HI GCNGC 8 cut(s) 249, 465, 1200, 1377, 1901, 2085, 2577, 2580
FokI GGATG 4 cut(s) 35, 745, 765, 1247
FriOI GRGCYC 1 cut(s) 577
Fsp4HI GCNGC 8 cut(s) 249, 465, 1200, 1377, 1901, 2085, 2577, 2580
FspBI CTAG 4 cut(s) 384, 1043, 2061, 2573
GluI GCNGC 8 cut(s) 249, 465, 1200, 1377, 1901, 2085, 2577, 2580
GsaI CCCAGC 1 cut(s) 344
HaeIII GGCC 5 cut(s) 841, 1243, 2358, 2381, 2434
HapII CCGG 2 cut(s) 1078, 1644
HgaI GACGC 2 cut(s) 563, 1484
HincII GTYRAC 2 cut(s) 1638, 1884
HindII GTYRAC 2 cut(s) 1638, 1884
HindIII AAGCTT 3 cut(s) 1101, 1621, 1867
HpaI GTTAAC 1 cut(s) 1884
HpaII CCGG 2 cut(s) 1078, 1644
HphI GGTGA 3 cut(s) 94, 208, 1804
Hpy166II GTNNAC 8 cut(s) 553, 916, 1251, 1638, 1795, 1884, 2145, 2344
Hpy8I GTNNAC 8 cut(s) 553, 916, 1251, 1638, 1795, 1884, 2145, 2344
HpyAV CCTTC 9 cut(s) 75, 342, 737, 761, 967, 1432, 1663, 2200, 2561
HpyCH4III ACNGT 6 cut(s) 946, 1095, 1583, 1888, 2058, 2632
HpyCH4IV ACGT 2 cut(s) 1532, 2241
HpyF10VI GCNNNNNNNGC 9 cut(s) 185, 257, 464, 725, 1871, 2355, 2378, 2440, 2588
HpyF3I CTNAG 8 cut(s) 124, 180, 1064, 1619, 1704, 1870, 2448, 2611
HpySE526I ACGT 2 cut(s) 1532, 2241
KspAI GTTAAC 1 cut(s) 1884
LmnI GCTCC 5 cut(s) 131, 476, 572, 634, 1196
Lsp1109I GCAGC 8 cut(s) 260, 451, 1211, 1363, 1912, 2071, 2566, 2588
MabI ACCWGGT 1 cut(s) 2584
MaeI CTAG 4 cut(s) 384, 1043, 2061, 2573
MaeII ACGT 2 cut(s) 1532, 2241
MaeIII GTNAC 7 cut(s) 437, 760, 1533, 1856, 1940, 2008, 2242
MfeI CAATTG 2 cut(s) 1497, 1798
MflI RGATCY 2 cut(s) 787, 2503
MhlI GDGCHC 3 cut(s) 577, 1692, 2456
MlsI TGGCCA 2 cut(s) 1243, 2434
MluNI TGGCCA 2 cut(s) 1243, 2434
MlyI GAGTC 4 cut(s) 434, 806, 2239, 2702
MmeI TCCRAC 7 cut(s) 454, 471, 550, 958, 1077, 1292, 2134
Mox20I TGGCCA 2 cut(s) 1243, 2434
MroXI GAANNNNTTC 2 cut(s) 17, 498
MscI TGGCCA 2 cut(s) 1243, 2434
MslI CAYNNNNRTG 3 cut(s) 155, 612, 2630
Msp20I TGGCCA 2 cut(s) 1243, 2434
MspA1I CMGCKG 2 cut(s) 2209, 2579
MspI CCGG 2 cut(s) 1078, 1644
MspR9I CCNGG 5 cut(s) 54, 220, 1245, 1427, 2586
MunI CAATTG 2 cut(s) 1497, 1798
Mva1269I GAATGC 1 cut(s) 1907
MvaI CCWGG 5 cut(s) 54, 220, 1245, 1427, 2586
MvnI CGCG 1 cut(s) 1695
MwoI GCNNNNNNNGC 9 cut(s) 185, 257, 464, 725, 1871, 2355, 2378, 2440, 2588
NcoI CCATGG 4 cut(s) 156, 807, 2148, 2385
NdeI CATATG 3 cut(s) 681, 937, 1408
NlaIV GGNNCC 5 cut(s) 700, 1163, 1966, 2022, 2328
NmuCI GTSAC 3 cut(s) 437, 2008, 2242
NspI RCATGY 4 cut(s) 188, 2224, 2410, 2549
NspV TTCGAA 1 cut(s) 1329
PaeI GCATGC 1 cut(s) 188
PaeR7I CTCGAG 3 cut(s) 576, 657, 1226
PasI CCCWGGG 1 cut(s) 53
PceI AGGCCT 1 cut(s) 2358
PciI ACATGT 2 cut(s) 2220, 2406
PctI GAATGC 1 cut(s) 1907
PdmI GAANNNNTTC 2 cut(s) 17, 498
PfeI GAWTC 7 cut(s) 224, 352, 1024, 1353, 1547, 2534, 2687
PkrI GCNGC 8 cut(s) 250, 466, 1201, 1378, 1902, 2086, 2578, 2581
PleI GAGTC 4 cut(s) 434, 806, 2239, 2702
PpsI GAGTC 4 cut(s) 434, 806, 2239, 2702
PpuMI RGGWCCY 1 cut(s) 1715
PscI ACATGT 2 cut(s) 2220, 2406
PsiI TTATAA 1 cut(s) 612
Psp124BI GAGCTC 1 cut(s) 577
Psp5II RGGWCCY 1 cut(s) 1715
Psp6I CCWGG 5 cut(s) 52, 218, 1243, 1425, 2584
PspFI CCCAGC 1 cut(s) 340
PspGI CCWGG 5 cut(s) 52, 218, 1243, 1425, 2584
PspN4I GGNNCC 5 cut(s) 700, 1163, 1966, 2022, 2328
PspPI GGNCC 6 cut(s) 302, 676, 699, 1715, 2156, 2327
PspPPI RGGWCCY 1 cut(s) 1715
PspXI VCTCGAGB 2 cut(s) 576, 657
PsrI GAACNNNNNNTAC 4 cut(s) 90, 122, 1665, 1697
PstI CTGCAG 1 cut(s) 1381
PsuI RGATCY 2 cut(s) 787, 2503
PvuII CAGCTG 1 cut(s) 2579
RsaI GTAC 8 cut(s) 93, 582, 1250, 1890, 2144, 2405, 2544, 2703
RsaNI GTAC 8 cut(s) 92, 581, 1249, 1889, 2143, 2404, 2543, 2702
RseI CAYNNNNRTG 3 cut(s) 155, 612, 2630
SacI GAGCTC 1 cut(s) 577
SatI GCNGC 8 cut(s) 249, 465, 1200, 1377, 1901, 2085, 2577, 2580
Sau96I GGNCC 6 cut(s) 302, 676, 699, 1715, 2156, 2327
SchI GAGTC 4 cut(s) 434, 806, 2239, 2702
ScrFI CCNGG 5 cut(s) 54, 220, 1245, 1427, 2586
SduI GDGCHC 3 cut(s) 577, 1692, 2456
SexAI ACCWGGT 1 cut(s) 2584
SfcI CTRYAG 3 cut(s) 1254, 1377, 2344
Sfr274I CTCGAG 3 cut(s) 576, 657, 1226
SfuI TTCGAA 1 cut(s) 1329
SinI GGWCC 6 cut(s) 302, 676, 699, 1715, 2156, 2327
SlaI CTCGAG 3 cut(s) 576, 657, 1226
SmiMI CAYNNNNRTG 3 cut(s) 155, 612, 2630
SmlI CTYRAG 6 cut(s) 576, 657, 1226, 1685, 2111, 2247
SmoI CTYRAG 6 cut(s) 576, 657, 1226, 1685, 2111, 2247
SpeI ACTAGT 1 cut(s) 383
SphI GCATGC 1 cut(s) 188
SseBI AGGCCT 1 cut(s) 2358
SsiI CCGC 3 cut(s) 1134, 1693, 2207
SspI AATATT 1 cut(s) 669
SspMI CTAG 4 cut(s) 384, 1043, 2061, 2573
SstI GAGCTC 1 cut(s) 577
StuI AGGCCT 1 cut(s) 2358
StyD4I CCNGG 5 cut(s) 52, 218, 1243, 1425, 2584
StyI CCWWGG 4 cut(s) 156, 807, 2148, 2385
TaaI ACNGT 6 cut(s) 946, 1095, 1583, 1888, 2058, 2632
TaiI ACGT 2 cut(s) 1535, 2244
TaqI TCGA 9 cut(s) 60, 577, 658, 776, 1227, 1329, 1510, 2225, 2706
TaqII GACCGA 1 cut(s) 755
TatI WGTACW 5 cut(s) 1888, 2142, 2403, 2542, 2701
TfiI GAWTC 7 cut(s) 224, 352, 1024, 1353, 1547, 2534, 2687
TscAI CASTG 9 cut(s) 132, 972, 1072, 1588, 1708, 1867, 1918, 2061, 2637
TseFI GTSAC 3 cut(s) 437, 2008, 2242
TseI GCWGC 8 cut(s) 248, 464, 1199, 1376, 1900, 2084, 2576, 2579
Tsp45I GTSAC 3 cut(s) 437, 2008, 2242
TspGWI ACGGA 3 cut(s) 533, 779, 2699
TspRI CASTG 9 cut(s) 132, 972, 1072, 1588, 1708, 1867, 1918, 2061, 2637
VpaK11BI GGWCC 6 cut(s) 302, 676, 699, 1715, 2156, 2327
XagI CCTNNNNNAGG 1 cut(s) 2565
XapI RAATTY 9 cut(s) 118, 624, 1052, 1072, 1316, 1325, 1455, 2000, 2313
XbaI TCTAGA 1 cut(s) 2060
XceI RCATGY 4 cut(s) 188, 2224, 2410, 2549
XcmI CCANNNNNNNNNTGG 1 cut(s) 2146
XhoI CTCGAG 3 cut(s) 576, 657, 1226
XmiI GTMKAC 2 cut(s) 552, 2343
XmnI GAANNNNTTC 2 cut(s) 17, 498
XspI CTAG 4 cut(s) 384, 1043, 2061, 2573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.