RchiOBHm_Chr4g0404001

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001930
Physical Location & Seq
Forward (+)
23499782 .. 23500892
1111 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37563

Sequence Viewer

Length: 795 bp
ATGCAGAAATATGACGCTGTGGTGGGAGACACAACAATTATAGCTGATTGCTCATTATATGTAGATTTTACGTTGCCTTATTCTGAATCAGGAGTTTCCATGATAGTTTTGACAGAGGACAGTGAGAGGAATAGTCTTTGGATTTTCTTGAAGCCACTAAGCTTGGATCTTTGGTTGACAACCGGCGTAGCCTTCATATTTACAGGTTTTGTAATATGGCTTCTTGAGCACCGGGAAAACACCGAGTTCAGAAGTCCTGCACAGCAACAACTTGGCATGATATTCTGGTTCTCGTTTTCAACCCTCGTCTTTGCACACAGTAAGTTCTCTTGTCCTTGGCAAGCATTTCTTTACACTGCAAGATTAGCCTCAATGTTAACAGTACAGAAGTTGCAGCCTGCATTCACTGATAGAAAAGAGATACAAAGAAATGGTTACAAAGTAGGATATCAAAATAGTTCCTTTATTAAAGGGTTTCTCGTGGAACATTTGCATTTTGCTGAGGCCAATTTGGTGCCATTGGTAACCATTCCGGATTATCATGAGGCACTATCTAGAGGAAACAAGAATAGAGTTGCTGCCATTTTAGATGAAGTCCCTTGCCTCAAGTTGTTCCTTAAAAAGAACTGTTCCAAGTACACCATGGTTGGACCAACCTACAAAACTGATGGATTTGGCTTTGCTTTCCCATCAAGGTCCTCGCTGGTATCCTACATGTCAAGGGCAATCTTCAACGTGACTCAAGATAAAAATGAGATGGATAAAATTCAGTCGACTTACTTTGATTACTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

264

Amino Acids

30.27

Weight (kDa)

6.95

Isoelectric Point (pI)

34.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lig_chan PF00060 54 - 105 6.2e-11 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 514
AccI GTMKAC 1 cut(s) 773
AccIII TCCGGA 1 cut(s) 532
AclWI GGATC 1 cut(s) 174
AcsI RAATTY 1 cut(s) 765
AfaI GTAC 2 cut(s) 384, 638
AflIII ACRYGT 1 cut(s) 714
AgsI TTSAA 3 cut(s) 151, 300, 733
AluBI AGCT 2 cut(s) 44, 162
AluI AGCT 2 cut(s) 44, 162
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 1 cut(s) 21
AlwI GGATC 1 cut(s) 174
Aor13HI TCCGGA 1 cut(s) 532
AoxI GGCC 1 cut(s) 504
ApeKI GCWGC 2 cut(s) 394, 578
ApoI RAATTY 1 cut(s) 765
AspS9I GGNCC 2 cut(s) 650, 696
AsuC2I CCSGG 1 cut(s) 233
AvaII GGWCC 2 cut(s) 650, 696
BanI GGYRCC 1 cut(s) 514
BarI GAAGNNNNNNTAC 2 cut(s) 204, 236
BauI CACGAG 1 cut(s) 479
Bbv12I GWGCWC 1 cut(s) 231
BbvCI CCTCAGC 1 cut(s) 501
BbvI GCAGC 2 cut(s) 406, 565
BccI CCATC 3 cut(s) 662, 697, 751
BciVI GTATCC 1 cut(s) 718
BcnI CCSGG 1 cut(s) 233
BcoDI GTCTC 1 cut(s) 21
BfaI CTAG 1 cut(s) 555
BfuI GTATCC 1 cut(s) 718
BisI GCNGC 2 cut(s) 395, 579
BlsI GCNGC 2 cut(s) 396, 580
Bme1390I CCNGG 1 cut(s) 233
Bme18I GGWCC 2 cut(s) 650, 696
BmgT120I GGNCC 2 cut(s) 650, 696
BmiI GGNNCC 1 cut(s) 516
BmrFI CCNGG 1 cut(s) 233
Bpu10I CCTNAGC 1 cut(s) 501
BpuEI CTTGAG 3 cut(s) 245, 590, 726
BpuMI CCSGG 1 cut(s) 233
BsaJI CCNNGG 2 cut(s) 335, 642
BsaWI WCCGGW 1 cut(s) 532
Bse118I RCCGGY 1 cut(s) 182
BseAI TCCGGA 1 cut(s) 532
BseDI CCNNGG 2 cut(s) 335, 642
BseMII CTCAG 1 cut(s) 492
BseXI GCAGC 2 cut(s) 406, 565
BsgI GTGCAG 1 cut(s) 243
BshFI GGCC 1 cut(s) 506
BshNI GGYRCC 1 cut(s) 514
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 3 cut(s) 183, 232, 533
BslFI GGGAC 1 cut(s) 581
BsmAI GTCTC 1 cut(s) 21
BsmFI GGGAC 1 cut(s) 581
BsmI GAATGC 1 cut(s) 401
BsnI GGCC 1 cut(s) 506
Bsp1286I GDGCHC 1 cut(s) 231
Bsp13I TCCGGA 1 cut(s) 532
Bsp143I GATC 1 cut(s) 166
Bsp19I CCATGG 1 cut(s) 642
BspANI GGCC 1 cut(s) 506
BspCNI CTCAG 1 cut(s) 493
BspEI TCCGGA 1 cut(s) 532
BspHI TCATGA 1 cut(s) 541
BspLI GGNNCC 1 cut(s) 516
BspPI GGATC 1 cut(s) 174
BspT107I GGYRCC 1 cut(s) 514
BsrFI RCCGGY 1 cut(s) 182
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 2 cut(s) 335, 642
BssMI GATC 1 cut(s) 166
BssSI CACGAG 1 cut(s) 479
BssT1I CCWWGG 2 cut(s) 335, 642
Bst2BI CACGAG 1 cut(s) 479
Bst4CI ACNGT 5 cut(s) 122, 320, 382, 629, 791
BstC8I GCNNGC 2 cut(s) 342, 399
BstDEI CTNAG 2 cut(s) 158, 501
BstDSI CCRYGG 1 cut(s) 642
BstEII GGTNACC 1 cut(s) 523
BstKTI GATC 1 cut(s) 169
BstMAI GTCTC 1 cut(s) 21
BstMBI GATC 1 cut(s) 166
BstMWI GCNNNNNNNGC 2 cut(s) 226, 365
BstNSI RCATGY 1 cut(s) 718
BstPI GGTNACC 1 cut(s) 523
BstSCI CCNGG 1 cut(s) 231
BstV1I GCAGC 2 cut(s) 406, 565
BstX2I RGATCY 1 cut(s) 166
BstYI RGATCY 1 cut(s) 166
BsuI GTATCC 1 cut(s) 718
BsuRI GGCC 1 cut(s) 506
BtgI CCRYGG 1 cut(s) 642
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 3 cut(s) 127, 354, 405
Cac8I GCNNGC 2 cut(s) 342, 399
CciI TCATGA 1 cut(s) 541
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 2 cut(s) 650, 696
CseI GACGC 1 cut(s) 23
Csp6I GTAC 2 cut(s) 383, 637
CspCI CAANNNNNGTGG 2 cut(s) 144, 179
CviAII CATG 5 cut(s) 100, 277, 542, 643, 715
CviJI RGCY 9 cut(s) 44, 154, 162, 191, 220, 368, 397, 506, 678
CviKI_1 RGCY 9 cut(s) 44, 154, 162, 191, 220, 368, 397, 506, 678
CviQI GTAC 2 cut(s) 383, 637
DdeI CTNAG 2 cut(s) 158, 501
DpnI GATC 1 cut(s) 168
DpnII GATC 1 cut(s) 166
Eco130I CCWWGG 2 cut(s) 335, 642
Eco32I GATATC 1 cut(s) 449
Eco47I GGWCC 2 cut(s) 650, 696
Eco91I GGTNACC 1 cut(s) 523
EcoO109I RGGNCCY 1 cut(s) 696
EcoO65I GGTNACC 1 cut(s) 523
EcoRV GATATC 1 cut(s) 449
EcoT14I CCWWGG 2 cut(s) 335, 642
ErhI CCWWGG 2 cut(s) 335, 642
FaeI CATG 5 cut(s) 103, 280, 545, 646, 718
FalI AAGNNNNNCTT 2 cut(s) 333, 365
FaqI GGGAC 1 cut(s) 581
FatI CATG 5 cut(s) 99, 276, 541, 642, 714
FblI GTMKAC 1 cut(s) 773
Fnu4HI GCNGC 2 cut(s) 395, 579
Fsp4HI GCNGC 2 cut(s) 395, 579
FspBI CTAG 1 cut(s) 555
GluI GCNGC 2 cut(s) 395, 579
HaeIII GGCC 1 cut(s) 506
HapII CCGG 3 cut(s) 183, 232, 533
HgaI GACGC 1 cut(s) 23
Hin1II CATG 5 cut(s) 103, 280, 545, 646, 718
HincII GTYRAC 3 cut(s) 177, 378, 774
HindII GTYRAC 3 cut(s) 177, 378, 774
HindIII AAGCTT 1 cut(s) 160
HinfI GANTC 2 cut(s) 86, 739
HpaI GTTAAC 1 cut(s) 378
HpaII CCGG 3 cut(s) 183, 232, 533
Hpy166II GTNNAC 4 cut(s) 177, 378, 639, 774
Hpy188I TCNGA 2 cut(s) 85, 251
Hpy188III TCNNGA 7 cut(s) 90, 148, 224, 533, 542, 555, 743
Hpy8I GTNNAC 4 cut(s) 177, 378, 639, 774
HpyAV CCTTC 1 cut(s) 202
HpyCH4III ACNGT 5 cut(s) 122, 320, 382, 629, 791
HpyCH4IV ACGT 2 cut(s) 71, 735
HpyCH4V TGCA 7 cut(s) 4, 260, 314, 359, 394, 401, 493
HpyF10VI GCNNNNNNNGC 2 cut(s) 226, 365
HpyF3I CTNAG 2 cut(s) 158, 501
HpySE526I ACGT 2 cut(s) 71, 735
Hsp92II CATG 5 cut(s) 103, 280, 545, 646, 718
Kpn2I TCCGGA 1 cut(s) 532
KspAI GTTAAC 1 cut(s) 378
Kzo9I GATC 1 cut(s) 166
LpnPI CCDG 9 cut(s) 75, 189, 196, 245, 270, 271, 411, 546, 689
Lsp1109I GCAGC 2 cut(s) 406, 565
MaeI CTAG 1 cut(s) 555
MaeII ACGT 2 cut(s) 71, 735
MaeIII GTNAC 3 cut(s) 434, 523, 736
MalI GATC 1 cut(s) 168
MboI GATC 1 cut(s) 166
MboII GAAGA 1 cut(s) 721
MflI RGATCY 1 cut(s) 166
MhlI GDGCHC 1 cut(s) 231
MluCI AATT 3 cut(s) 36, 508, 765
MlyI GAGTC 1 cut(s) 733
MmeI TCCRAC 1 cut(s) 628
MnlI CCTC 9 cut(s) 109, 120, 314, 379, 496, 538, 551, 614, 709
MroI TCCGGA 1 cut(s) 532
MseI TTAA 4 cut(s) 377, 468, 618, 793
MspI CCGG 3 cut(s) 183, 232, 533
MspR9I CCNGG 1 cut(s) 233
Mva1269I GAATGC 1 cut(s) 401
MwoI GCNNNNNNNGC 2 cut(s) 226, 365
NciI CCSGG 1 cut(s) 233
NcoI CCATGG 1 cut(s) 642
NdeII GATC 1 cut(s) 166
NlaIII CATG 5 cut(s) 103, 280, 545, 646, 718
NlaIV GGNNCC 1 cut(s) 516
NmuCI GTSAC 1 cut(s) 736
NspI RCATGY 1 cut(s) 718
PagI TCATGA 1 cut(s) 541
PciI ACATGT 1 cut(s) 714
PctI GAATGC 1 cut(s) 401
PfeI GAWTC 1 cut(s) 86
PkrI GCNGC 2 cut(s) 396, 580
PleI GAGTC 1 cut(s) 733
PpsI GAGTC 1 cut(s) 733
PpuMI RGGWCCY 1 cut(s) 696
PscI ACATGT 1 cut(s) 714
Psp5II RGGWCCY 1 cut(s) 696
PspEI GGTNACC 1 cut(s) 523
PspN4I GGNNCC 1 cut(s) 516
PspPI GGNCC 2 cut(s) 650, 696
PspPPI RGGWCCY 1 cut(s) 696
PsuI RGATCY 1 cut(s) 166
RsaI GTAC 2 cut(s) 384, 638
RsaNI GTAC 2 cut(s) 383, 637
SalI GTCGAC 1 cut(s) 772
SaqAI TTAA 4 cut(s) 377, 468, 618, 793
SatI GCNGC 2 cut(s) 395, 579
Sau3AI GATC 1 cut(s) 166
Sau96I GGNCC 2 cut(s) 650, 696
SchI GAGTC 1 cut(s) 733
ScrFI CCNGG 1 cut(s) 233
SduI GDGCHC 1 cut(s) 231
SetI ASST 7 cut(s) 46, 74, 164, 208, 659, 698, 738
SinI GGWCC 2 cut(s) 650, 696
SmlI CTYRAG 3 cut(s) 224, 605, 741
SmoI CTYRAG 3 cut(s) 224, 605, 741
Sse9I AATT 3 cut(s) 36, 508, 765
SspMI CTAG 1 cut(s) 555
StyD4I CCNGG 1 cut(s) 231
StyI CCWWGG 2 cut(s) 335, 642
TaaI ACNGT 5 cut(s) 122, 320, 382, 629, 791
TaiI ACGT 2 cut(s) 74, 738
TaqI TCGA 1 cut(s) 773
TasI AATT 3 cut(s) 36, 508, 765
TatI WGTACW 2 cut(s) 382, 636
TfiI GAWTC 1 cut(s) 86
Tru1I TTAA 4 cut(s) 377, 468, 618, 793
Tru9I TTAA 4 cut(s) 377, 468, 618, 793
TscAI CASTG 3 cut(s) 127, 361, 412
TseFI GTSAC 1 cut(s) 736
TseI GCWGC 2 cut(s) 394, 578
Tsp45I GTSAC 1 cut(s) 736
TspDTI ATGAA 2 cut(s) 184, 606
TspRI CASTG 3 cut(s) 127, 361, 412
VpaK11BI GGWCC 2 cut(s) 650, 696
XapI RAATTY 1 cut(s) 765
XbaI TCTAGA 1 cut(s) 554
XceI RCATGY 1 cut(s) 718
XcmI CCANNNNNNNNNTGG 1 cut(s) 640
XmiI GTMKAC 1 cut(s) 773
XspI CTAG 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.