Rh4CG130800

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001930
Physical Location & Seq
Forward (+)
25689108 .. 25703467
14360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG130800.1

Sequence Viewer

Length: 2709 bp
ATGGCAAACCAAAAGCGCTTCCTCTGGTTCATCTTCATTATTTTGTTCAGCCCTGGGATCGAGCCTTCAACGACGAAAGAAGTGATACGAGTACCAGTAGGAGTTGTTCTTAACTTAAATTCCTCAGTGGGAGCTATGGCAGAGAAGTGCATGACCATGGCACTTGATGATTTTTATGCTAAGCATGCTCATTATCAAACAAGACTTTCCCTTCTCACCAGGGATTCAAAGAATGATGTTGTTTCGGCAGCATTTGAAGCGCTGCACTTGATAAACATTGAAAATATGAGTGCCATTATTGGACCTCAAAGTTCAGCAGAAGCGAAGCATGTGATAGAGCTGGGAAGAAGGAATCATGTTCCCATTCTTTCGTTTTCTGCCACTAGTCCATCTCTTTCTCCATCCCATAGCTCATTTTTCATTCGGACGGCTTTTAGTGACTCTGCTCAAGTAGAAGCCATGGCTGCCATTGTCGGAGCTTATAGTTGGTTGGAAGTTGTTCTCATTTATGAAGACACGGAGTATGGAAATAGCTTAATTCCATATTTGGAAGACGCTTTCCAGAAAGTGGGAGCTCGAGTACCTCATAGAAGTGTCATTCCTCATCATTATAATGACAGAGAAATTTTAAAGGAGCTTAACCGCTTGAATTCAACTCGAGCAAGAATATTTTTGGTCCATATGACTGCTTCCCTCGGGTCCAAATTGTTTTTATTAGCAAGTAAGGCAGGGATGATGACCGAAGGGTATGGATGGATTGTTACTGAAGGGTTATCGACTTTATTAGATCCTGTGGGTTCAAGAACCATGAACTCAATGCAAGGTGTAGTGGGAGTAAGGCCATATTTACCAATGTCAAAAGAACTTGAAGACTTCGTGTTGCGAATGAAAATATTCAGGTTCAGAAAATCAAGCAAGATTACTGGTGTAAACCTCTTTGGGTTGTGGGCATATGATACAGTTTGGGCATTAGCAATGGCAGTGGAGAAGGTTGGAATAGCAAGCAGAGTTGATCTTGCAAGTTTGGGAACTGGTGACAGGGGTCAAAATCTTCTTGAAGCTATCCTAGATTTAAAATTTGAAAACCTCAGTGGGAATTTCCGGTTGGTTAAGGGGCAGTTGGAAGCTTCAACCTTTGAGGTATTTAATGTGATTGGCGGAAAAGAAAGAATAATTGGATATTGGAACCAAAAGAAAGGACTTTCCCGAAAGCTGAATGGAGCAGCAGAGTTGGATAAAAGGAAGACACTCGAGAAACCAATCTGGCCAGGGTATACTACAGACATCCCTCCGAAATTGAGGATTGGAGTTCCCATGAAACAGGGTTTTAATGAATTCCTAAAATTCGAAAACAACCACCATATATCAGGATTCTCTGCTGATATATTCTTTGCTGCAGTAAAGCAACTGCCATTTTCCATTTCATATGAGTTTTCGGCCTTCGCGGGGACTTATGATGATCTTCTTTATCAAATTCATCTCCAGAAATATGACGCTGTGGTGGGAGACACAACAATTGTTGCTAATCGCTCATTGTATGTAGATTTTACGTTGCCTTATACTGAATCAGGAGTTTCCATGTTGGTTTTGACAGAAGATAGTGAGAGGAATAGTCTTTGGATTTTCTTGAAGCCACTAAGCTTGGATCTTTGGTTGACAACCGGCGTAGCCTTCATATTTACAGGTTTTGTAATATGGCTTCTTGAGCACCGGGAAAACACCGAGTTCAGAGGTCCTCCACAGCAACAACTTGGAATGATATTCTGGTTCTCCTTTTCAACCCTCGTCTTTGCACACAGAGAGAAAGTGGTGAACAATTGGTCAAGGTTTGTGCTGATTATATGGGTTTTTGTGGTACTGATCCTAACACAGAGTTACACTGCAAGCTTAGCCTCAATGTTAACAGTACAAAAGTTGCAGCCTGCATTCACTGATATAAAAGAGATACAAAGAAATGGTTACAAAGTGGGATATCAAAATAGTTCCTTTATTAAAGGGTTTCTCATGGAACATTTAAAGTTTAATGAGACCAAGTTGGAGCCATTGGAAACCATTCCGGATTATCATAACGCGCTGTCTAGAGGAAACAAGAATCGAGTTGCTGCCATTTTAGATGAAATCCCTTACCTCAAGTTGTTTCTTAAAACGAACTGTTCCAAGTACACCATGGTTGGACCAACCTACAAAACTGATGGACTTGGCTTTGCCTTCCCAATAGGGTCCCCGCTGGTATCCTACATGTCGAGGGCAATCTTGAACGTGACTCAAGACAAAAATCAGATGGATAGAATTCAGTCGACTTACTTTGATTACTTTGATAACAAACCTACCTGTGAGGATCAAAGTGCCAAAATTTCTTCAGAGGGTCCAAGTCTTCGTGTCTACAGCTTCGGAGGCCTCTTCATCATCGCAGGTGTGGCCTCCCTGGTTGCTCTTTTGATGTACATGTATCACTTCCTTTGCTCTCATTGGCCAGCTTTGCGAACCATTAGTTCTTCTGACAGTACATTTTGGGAGAAATTGGTTGAAGTGGCAAAGCATTTTGACAAGAAAGAACTCAATTCACATCAGTTTAAGCGAAGAGAATCAAGTGTACATGCTTTAGACACTCCTGGTGAAGGTCTGGCAGCTGCACCGGGTGCTAATGATATGCAAAACAACTCAGCAGGAACTCACATAACAGTGGAGAACAATCTTGGACATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

902

Amino Acids

101.5

Weight (kDa)

8.05

Isoelectric Point (pI)

35.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 41 - 366 1.4e-21 Periplasmic binding protein
ANF_receptor PF01094 48 - 386 3.3e-76 Receptor family ligand binding region
SBP_bac_3 PF00497 449 - 769 4.2e-09 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 547 - 807 2.1e-37 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 612
AarI CACCTGC 1 cut(s) 2402
Acc36I ACCTGC 1 cut(s) 2402
AccB7I CCANNNNNTGG 1 cut(s) 568
AccI GTMKAC 3 cut(s) 1274, 2297, 2382
AccII CGCG 2 cut(s) 1445, 2072
AccIII TCCGGA 1 cut(s) 2056
AciI CCGC 4 cut(s) 643, 1158, 1445, 2225
AclWI GGATC 5 cut(s) 65, 782, 1653, 1855, 2346
AcoI YGGCCR 2 cut(s) 1265, 2471
AcuI CTGAAG 2 cut(s) 786, 2343
AdeI CACNNNGTG 1 cut(s) 2639
AfaI GTAC 8 cut(s) 93, 582, 1857, 1908, 2162, 2444, 2506, 2595
AfeI AGCGCT 2 cut(s) 17, 261
AfiI CCNNNNNNNGG 3 cut(s) 568, 1446, 2618
AflIII ACRYGT 2 cut(s) 2238, 2445
AhlI ACTAGT 1 cut(s) 383
AjnI CCWGG 5 cut(s) 52, 218, 1267, 2424, 2611
AjuI GAANNNNNNNTTGG 4 cut(s) 1088, 1120, 1158, 1190
Alw21I GWGCWC 2 cut(s) 577, 1710
Alw26I GTCTC 2 cut(s) 1500, 2021
AlwI GGATC 5 cut(s) 65, 782, 1653, 1855, 2346
Ama87I CYCGRG 4 cut(s) 576, 657, 695, 1250
Aor13HI TCCGGA 1 cut(s) 2056
Aor51HI AGCGCT 2 cut(s) 17, 261
AoxI GGCC 6 cut(s) 839, 1265, 1437, 2395, 2418, 2471
ApeKI GCWGC 9 cut(s) 248, 262, 464, 1223, 1394, 1918, 2102, 2627, 2630
Asp700I GAANNNNTTC 3 cut(s) 498, 893, 2052
AspLEI GCGC 3 cut(s) 18, 262, 2074
AspS9I GGNCC 7 cut(s) 302, 676, 699, 1733, 2174, 2220, 2366
AsuC2I CCSGG 2 cut(s) 1712, 2637
AsuHPI GGTGA 4 cut(s) 208, 1046, 1822, 2627
AsuII TTCGAA 1 cut(s) 1347
AvaI CYCGRG 4 cut(s) 576, 657, 695, 1250
AvaII GGWCC 7 cut(s) 302, 676, 699, 1733, 2174, 2220, 2366
BalI TGGCCA 2 cut(s) 1267, 2473
BanII GRGCYC 1 cut(s) 577
BarI GAAGNNNNNNTAC 2 cut(s) 1683, 1715
BbsI GAAGAC 5 cut(s) 519, 558, 876, 1250, 2366
Bbv12I GWGCWC 2 cut(s) 577, 1710
BbvI GCAGC 9 cut(s) 249, 260, 451, 1235, 1381, 1930, 2089, 2617, 2639
BccI CCATC 5 cut(s) 397, 409, 747, 2186, 2275
BceAI ACGGC 1 cut(s) 444
BciT130I CCWGG 5 cut(s) 54, 220, 1269, 2426, 2613
BciVI GTATCC 1 cut(s) 2242
BcnI CCSGG 2 cut(s) 1712, 2637
BcoDI GTCTC 2 cut(s) 1500, 2021
BcuI ACTAGT 1 cut(s) 383
BfaI CTAG 3 cut(s) 384, 1067, 2079
BfmI CTRYAG 3 cut(s) 1278, 1395, 2383
BfoI RGCGCY 2 cut(s) 19, 263
BfuAI ACCTGC 1 cut(s) 2402
BfuI GTATCC 1 cut(s) 2242
BisI GCNGC 9 cut(s) 249, 263, 465, 1224, 1395, 1919, 2103, 2628, 2631
BlpI GCTNAGC 2 cut(s) 180, 1888
BlsI GCNGC 9 cut(s) 250, 264, 466, 1225, 1396, 1920, 2104, 2629, 2632
Bme1390I CCNGG 7 cut(s) 54, 220, 1269, 1712, 2426, 2613, 2637
Bme18I GGWCC 7 cut(s) 302, 676, 699, 1733, 2174, 2220, 2366
BmeT110I CYCGRG 4 cut(s) 576, 657, 695, 1250
BmgT120I GGNCC 7 cut(s) 302, 676, 699, 1733, 2174, 2220, 2366
BmiI GGNNCC 6 cut(s) 700, 1187, 2040, 2221, 2222, 2367
BmrFI CCNGG 7 cut(s) 54, 220, 1269, 1712, 2426, 2613, 2637
BoxI GACNNNNGTC 1 cut(s) 1041
BpiI GAAGAC 5 cut(s) 519, 558, 876, 1250, 2366
BpmI CTGGAG 1 cut(s) 1466
Bpu1102I GCTNAGC 2 cut(s) 180, 1888
Bpu14I TTCGAA 1 cut(s) 1347
BpuEI CTTGAG 4 cut(s) 432, 1724, 2114, 2250
BpuMI CCSGG 2 cut(s) 1712, 2637
BsaI GGTCTC 1 cut(s) 2021
BsaJI CCNNGG 9 cut(s) 52, 53, 156, 219, 459, 694, 1268, 2166, 2424
BsaWI WCCGGW 2 cut(s) 1101, 2056
BsaXI ACNNNNNCTCC 2 cut(s) 564, 594
Bsc4I CCNNNNNNNGG 3 cut(s) 568, 1446, 2618
Bse118I RCCGGY 1 cut(s) 1661
Bse1I ACTGG 3 cut(s) 95, 928, 1036
Bse3DI GCAATG 1 cut(s) 981
BseAI TCCGGA 1 cut(s) 2056
BseBI CCWGG 5 cut(s) 54, 220, 1269, 2426, 2613
BseDI CCNNGG 9 cut(s) 52, 53, 156, 219, 459, 694, 1268, 2166, 2424
BseGI GGATG 4 cut(s) 401, 738, 758, 1284
BseLI CCNNNNNNNGG 3 cut(s) 568, 1446, 2618
BseMI GCAATG 1 cut(s) 981
BseMII CTCAG 3 cut(s) 138, 1102, 2676
BseNI ACTGG 3 cut(s) 95, 928, 1036
BseXI GCAGC 9 cut(s) 249, 260, 451, 1235, 1381, 1930, 2089, 2617, 2639
BseYI CCCAGC 1 cut(s) 340
BsgI GTGCAG 2 cut(s) 248, 2616
Bsh1236I CGCG 2 cut(s) 1445, 2072
BshFI GGCC 6 cut(s) 841, 1267, 1439, 2397, 2420, 2473
BsiHKAI GWGCWC 2 cut(s) 577, 1710
BsiHKCI CYCGRG 4 cut(s) 576, 657, 695, 1250
BsiSI CCGG 5 cut(s) 1102, 1662, 1711, 2057, 2636
BslFI GGGAC 2 cut(s) 1462, 2206
BslI CCNNNNNNNGG 3 cut(s) 568, 1446, 2618
BsmAI GTCTC 2 cut(s) 1500, 2021
BsmFI GGGAC 2 cut(s) 1462, 2206
BsmI GAATGC 1 cut(s) 1925
BsnI GGCC 6 cut(s) 841, 1267, 1439, 2397, 2420, 2473
Bso31I GGTCTC 1 cut(s) 2021
BsoBI CYCGRG 4 cut(s) 576, 657, 695, 1250
Bsp119I TTCGAA 1 cut(s) 1347
Bsp1286I GDGCHC 2 cut(s) 577, 1710
Bsp13I TCCGGA 1 cut(s) 2056
Bsp1407I TGTACA 2 cut(s) 2442, 2593
Bsp143I GATC 7 cut(s) 57, 787, 1012, 1459, 1645, 1860, 2338
Bsp1720I GCTNAGC 2 cut(s) 180, 1888
Bsp19I CCATGG 3 cut(s) 156, 459, 2166
BspACI CCGC 4 cut(s) 643, 1158, 1445, 2225
BspANI GGCC 6 cut(s) 841, 1267, 1439, 2397, 2420, 2473
BspCNI CTCAG 3 cut(s) 137, 1101, 2675
BspEI TCCGGA 1 cut(s) 2056
BspFNI CGCG 2 cut(s) 1445, 2072
BspLI GGNNCC 6 cut(s) 700, 1187, 2040, 2221, 2222, 2367
BspMAI CTGCAG 1 cut(s) 1399
BspMI ACCTGC 1 cut(s) 2402
BspPI GGATC 5 cut(s) 65, 782, 1653, 1855, 2346
BspT104I TTCGAA 1 cut(s) 1347
BspTNI GGTCTC 1 cut(s) 2021
BsrDI GCAATG 1 cut(s) 981
BsrFI RCCGGY 1 cut(s) 1661
BsrGI TGTACA 2 cut(s) 2442, 2593
BsrI ACTGG 3 cut(s) 95, 928, 1036
BssAI RCCGGY 1 cut(s) 1661
BssECI CCNNGG 9 cut(s) 52, 53, 156, 219, 459, 694, 1268, 2166, 2424
BssMI GATC 7 cut(s) 57, 787, 1012, 1459, 1645, 1860, 2338
BssNAI GTATAC 1 cut(s) 1275
BssT1I CCWWGG 3 cut(s) 156, 459, 2166
Bst1107I GTATAC 1 cut(s) 1275
Bst2UI CCWGG 5 cut(s) 54, 220, 1269, 2426, 2613
Bst4CI ACNGT 5 cut(s) 961, 1906, 2153, 2504, 2683
Bst6I CTCTTC 2 cut(s) 2405, 2575
BstAPI GCANNNNNTGC 1 cut(s) 2639
BstAUI TGTACA 2 cut(s) 2442, 2593
BstBI TTCGAA 1 cut(s) 1347
BstC8I GCNNGC 5 cut(s) 186, 1003, 1885, 1923, 2475
BstDEI CTNAG 6 cut(s) 124, 180, 1088, 1637, 1888, 2662
BstDSI CCRYGG 3 cut(s) 156, 459, 2166
BstENI CCTNNNNNAGG 1 cut(s) 2616
BstF5I GGATG 4 cut(s) 401, 738, 758, 1284
BstFNI CGCG 2 cut(s) 1445, 2072
BstH2I RGCGCY 2 cut(s) 19, 263
BstHHI GCGC 3 cut(s) 18, 262, 2074
BstKTI GATC 7 cut(s) 60, 790, 1015, 1462, 1648, 1863, 2341
BstMAI GTCTC 2 cut(s) 1500, 2021
BstMBI GATC 7 cut(s) 57, 787, 1012, 1459, 1645, 1860, 2338
BstNI CCWGG 5 cut(s) 54, 220, 1269, 2426, 2613
BstNSI RCATGY 5 cut(s) 188, 332, 2242, 2449, 2600
BstPAI GACNNNNGTC 1 cut(s) 1041
BstSCI CCNGG 7 cut(s) 52, 218, 1267, 1710, 2424, 2611, 2635
BstSFI CTRYAG 3 cut(s) 1278, 1395, 2383
BstUI CGCG 2 cut(s) 1445, 2072
BstV1I GCAGC 9 cut(s) 249, 260, 451, 1235, 1381, 1930, 2089, 2617, 2639
BstV2I GAAGAC 5 cut(s) 519, 558, 876, 1250, 2366
BstX2I RGATCY 2 cut(s) 787, 1645
BstYI RGATCY 2 cut(s) 787, 1645
BstZ17I GTATAC 1 cut(s) 1275
BsuI GTATCC 1 cut(s) 2242
BsuRI GGCC 6 cut(s) 841, 1267, 1439, 2397, 2420, 2473
BtgI CCRYGG 3 cut(s) 156, 459, 2166
BtgZI GCGATG 1 cut(s) 2392
BtsCI GGATG 4 cut(s) 401, 738, 758, 1284
BtsI GCAGTG 2 cut(s) 987, 1878
BtsIMutI CAGTG 6 cut(s) 132, 987, 1096, 1878, 1929, 2688
BveI ACCTGC 1 cut(s) 2402
Cac8I GCNNGC 5 cut(s) 186, 1003, 1885, 1923, 2475
CfoI GCGC 3 cut(s) 18, 262, 2074
Cfr10I RCCGGY 1 cut(s) 1661
Cfr13I GGNCC 7 cut(s) 302, 676, 699, 1733, 2174, 2220, 2366
CseI GACGC 2 cut(s) 563, 1502
Csp6I GTAC 8 cut(s) 92, 581, 1856, 1907, 2161, 2443, 2505, 2594
CspCI CAANNNNNGTGG 4 cut(s) 963, 998, 1623, 1658
CviQI GTAC 8 cut(s) 92, 581, 1856, 1907, 2161, 2443, 2505, 2594
DdeI CTNAG 6 cut(s) 124, 180, 1088, 1637, 1888, 2662
DpnI GATC 7 cut(s) 59, 789, 1014, 1461, 1647, 1862, 2340
DpnII GATC 7 cut(s) 57, 787, 1012, 1459, 1645, 1860, 2338
DraI TTTAAA 3 cut(s) 630, 1074, 2016
DraIII CACNNNGTG 1 cut(s) 2639
EaeI YGGCCR 2 cut(s) 1265, 2471
Eam1104I CTCTTC 2 cut(s) 2405, 2575
EarI CTCTTC 2 cut(s) 2405, 2575
EciI GGCGGA 1 cut(s) 1173
Ecl136II GAGCTC 1 cut(s) 575
Eco130I CCWWGG 3 cut(s) 156, 459, 2166
Eco147I AGGCCT 1 cut(s) 2397
Eco24I GRGCYC 1 cut(s) 577
Eco31I GGTCTC 1 cut(s) 2021
Eco32I GATATC 1 cut(s) 1973
Eco47I GGWCC 7 cut(s) 302, 676, 699, 1733, 2174, 2220, 2366
Eco47III AGCGCT 2 cut(s) 17, 261
Eco53kI GAGCTC 1 cut(s) 575
Eco57I CTGAAG 2 cut(s) 786, 2343
Eco88I CYCGRG 4 cut(s) 576, 657, 695, 1250
EcoICRI GAGCTC 1 cut(s) 575
EcoNI CCTNNNNNAGG 1 cut(s) 2616
EcoO109I RGGNCCY 2 cut(s) 1733, 2220
EcoRI GAATTC 3 cut(s) 649, 1334, 2289
EcoRII CCWGG 5 cut(s) 52, 218, 1267, 2424, 2611
EcoRV GATATC 1 cut(s) 1973
EcoT14I CCWWGG 3 cut(s) 156, 459, 2166
EcoT38I GRGCYC 1 cut(s) 577
ErhI CCWWGG 3 cut(s) 156, 459, 2166
FaqI GGGAC 2 cut(s) 1462, 2206
FauI CCCGC 2 cut(s) 1438, 2232
FauNDI CATATG 3 cut(s) 681, 952, 1426
FblI GTMKAC 3 cut(s) 1274, 2297, 2382
Fnu4HI GCNGC 9 cut(s) 249, 263, 465, 1224, 1395, 1919, 2103, 2628, 2631
FokI GGATG 4 cut(s) 388, 745, 765, 1271
FriOI GRGCYC 1 cut(s) 577
Fsp4HI GCNGC 9 cut(s) 249, 263, 465, 1224, 1395, 1919, 2103, 2628, 2631
FspBI CTAG 3 cut(s) 384, 1067, 2079
GlaI GCGC 3 cut(s) 17, 261, 2073
GluI GCNGC 9 cut(s) 249, 263, 465, 1224, 1395, 1919, 2103, 2628, 2631
GsaI CCCAGC 1 cut(s) 344
GsuI CTGGAG 1 cut(s) 1466
HaeII RGCGCY 2 cut(s) 19, 263
HaeIII GGCC 6 cut(s) 841, 1267, 1439, 2397, 2420, 2473
HapII CCGG 5 cut(s) 1102, 1662, 1711, 2057, 2636
HgaI GACGC 2 cut(s) 563, 1502
HhaI GCGC 3 cut(s) 18, 262, 2074
Hin6I GCGC 3 cut(s) 16, 260, 2072
HinP1I GCGC 3 cut(s) 16, 260, 2072
HincII GTYRAC 3 cut(s) 1656, 1902, 2298
HindII GTYRAC 3 cut(s) 1656, 1902, 2298
HindIII AAGCTT 3 cut(s) 1125, 1639, 1885
HinfI GANTC 8 cut(s) 224, 352, 440, 1371, 1565, 2092, 2263, 2585
HpaI GTTAAC 1 cut(s) 1902
HpaII CCGG 5 cut(s) 1102, 1662, 1711, 2057, 2636
HphI GGTGA 4 cut(s) 208, 1046, 1822, 2627
Hpy166II GTNNAC 9 cut(s) 931, 1275, 1656, 1813, 1902, 2163, 2298, 2383, 2594
Hpy188I TCNGA 9 cut(s) 426, 476, 905, 1293, 1730, 2280, 2362, 2393, 2500
Hpy8I GTNNAC 9 cut(s) 931, 1275, 1656, 1813, 1902, 2163, 2298, 2383, 2594
Hpy99I CGWCG 1 cut(s) 76
HpyCH4III ACNGT 5 cut(s) 961, 1906, 2153, 2504, 2683
HpyCH4IV ACGT 2 cut(s) 1550, 2259
HpyF3I CTNAG 6 cut(s) 124, 180, 1088, 1637, 1888, 2662
HpySE526I ACGT 2 cut(s) 1550, 2259
HspAI GCGC 3 cut(s) 16, 260, 2072
KflI GGGWCCC 1 cut(s) 2220
Kpn2I TCCGGA 1 cut(s) 2056
KspAI GTTAAC 1 cut(s) 1902
Kzo9I GATC 7 cut(s) 57, 787, 1012, 1459, 1645, 1860, 2338
LmnI GCTCC 6 cut(s) 131, 476, 572, 634, 1220, 2038
Lsp1109I GCAGC 9 cut(s) 249, 260, 451, 1235, 1381, 1930, 2089, 2617, 2639
MaeI CTAG 3 cut(s) 384, 1067, 2079
MaeII ACGT 2 cut(s) 1550, 2259
MaeIII GTNAC 6 cut(s) 437, 760, 1034, 1874, 1958, 2260
MalI GATC 7 cut(s) 59, 789, 1014, 1461, 1647, 1862, 2340
MboI GATC 7 cut(s) 57, 787, 1012, 1459, 1645, 1860, 2338
MfeI CAATTG 2 cut(s) 1515, 1816
MflI RGATCY 2 cut(s) 787, 1645
MhlI GDGCHC 2 cut(s) 577, 1710
MlsI TGGCCA 2 cut(s) 1267, 2473
MluNI TGGCCA 2 cut(s) 1267, 2473
MlyI GAGTC 2 cut(s) 434, 2257
MmeI TCCRAC 7 cut(s) 454, 471, 973, 1101, 1212, 2016, 2152
Mox20I TGGCCA 2 cut(s) 1267, 2473
MroI TCCGGA 1 cut(s) 2056
MroXI GAANNNNTTC 3 cut(s) 498, 893, 2052
MscI TGGCCA 2 cut(s) 1267, 2473
MslI CAYNNNNRTG 4 cut(s) 155, 591, 612, 2681
Msp20I TGGCCA 2 cut(s) 1267, 2473
MspA1I CMGCKG 2 cut(s) 2227, 2630
MspI CCGG 5 cut(s) 1102, 1662, 1711, 2057, 2636
MspR9I CCNGG 7 cut(s) 54, 220, 1269, 1712, 2426, 2613, 2637
MunI CAATTG 2 cut(s) 1515, 1816
Mva1269I GAATGC 1 cut(s) 1925
MvaI CCWGG 5 cut(s) 54, 220, 1269, 2426, 2613
MvnI CGCG 2 cut(s) 1445, 2072
NciI CCSGG 2 cut(s) 1712, 2637
NcoI CCATGG 3 cut(s) 156, 459, 2166
NdeI CATATG 3 cut(s) 681, 952, 1426
NdeII GATC 7 cut(s) 57, 787, 1012, 1459, 1645, 1860, 2338
NlaIV GGNNCC 6 cut(s) 700, 1187, 2040, 2221, 2222, 2367
NmuCI GTSAC 3 cut(s) 437, 1034, 2260
NspI RCATGY 5 cut(s) 188, 332, 2242, 2449, 2600
NspV TTCGAA 1 cut(s) 1347
PaeI GCATGC 1 cut(s) 188
PaeR7I CTCGAG 3 cut(s) 576, 657, 1250
PaqCI CACCTGC 1 cut(s) 2402
PasI CCCWGGG 1 cut(s) 53
PceI AGGCCT 1 cut(s) 2397
PciI ACATGT 2 cut(s) 2238, 2445
PctI GAATGC 1 cut(s) 1925
PdmI GAANNNNTTC 3 cut(s) 498, 893, 2052
PfeI GAWTC 6 cut(s) 224, 352, 1371, 1565, 2092, 2585
PflMI CCANNNNNTGG 1 cut(s) 568
PkrI GCNGC 9 cut(s) 250, 264, 466, 1225, 1396, 1920, 2104, 2629, 2632
PleI GAGTC 2 cut(s) 434, 2257
PpsI GAGTC 2 cut(s) 434, 2257
PpuMI RGGWCCY 2 cut(s) 1733, 2220
PscI ACATGT 2 cut(s) 2238, 2445
PshAI GACNNNNGTC 1 cut(s) 1041
PsiI TTATAA 1 cut(s) 612
Psp124BI GAGCTC 1 cut(s) 577
Psp5II RGGWCCY 2 cut(s) 1733, 2220
Psp6I CCWGG 5 cut(s) 52, 218, 1267, 2424, 2611
PspFI CCCAGC 1 cut(s) 340
PspGI CCWGG 5 cut(s) 52, 218, 1267, 2424, 2611
PspN4I GGNNCC 6 cut(s) 700, 1187, 2040, 2221, 2222, 2367
PspPI GGNCC 7 cut(s) 302, 676, 699, 1733, 2174, 2220, 2366
PspPPI RGGWCCY 2 cut(s) 1733, 2220
PspXI VCTCGAGB 2 cut(s) 576, 657
PsrI GAACNNNNNNTAC 2 cut(s) 90, 122
PstI CTGCAG 1 cut(s) 1399
PsuI RGATCY 2 cut(s) 787, 1645
PvuII CAGCTG 1 cut(s) 2630
RsaI GTAC 8 cut(s) 93, 582, 1857, 1908, 2162, 2444, 2506, 2595
RsaNI GTAC 8 cut(s) 92, 581, 1856, 1907, 2161, 2443, 2505, 2594
RseI CAYNNNNRTG 4 cut(s) 155, 591, 612, 2681
SacI GAGCTC 1 cut(s) 577
SalI GTCGAC 1 cut(s) 2296
SatI GCNGC 9 cut(s) 249, 263, 465, 1224, 1395, 1919, 2103, 2628, 2631
Sau3AI GATC 7 cut(s) 57, 787, 1012, 1459, 1645, 1860, 2338
Sau96I GGNCC 7 cut(s) 302, 676, 699, 1733, 2174, 2220, 2366
SchI GAGTC 2 cut(s) 434, 2257
ScrFI CCNGG 7 cut(s) 54, 220, 1269, 1712, 2426, 2613, 2637
SduI GDGCHC 2 cut(s) 577, 1710
SfcI CTRYAG 3 cut(s) 1278, 1395, 2383
Sfr274I CTCGAG 3 cut(s) 576, 657, 1250
SfuI TTCGAA 1 cut(s) 1347
SinI GGWCC 7 cut(s) 302, 676, 699, 1733, 2174, 2220, 2366
SlaI CTCGAG 3 cut(s) 576, 657, 1250
SmiMI CAYNNNNRTG 4 cut(s) 155, 591, 612, 2681
SmlI CTYRAG 7 cut(s) 447, 576, 657, 1250, 1703, 2129, 2265
SmoI CTYRAG 7 cut(s) 447, 576, 657, 1250, 1703, 2129, 2265
SpeI ACTAGT 1 cut(s) 383
SphI GCATGC 1 cut(s) 188
SseBI AGGCCT 1 cut(s) 2397
SsiI CCGC 4 cut(s) 643, 1158, 1445, 2225
SspI AATATT 2 cut(s) 669, 894
SspMI CTAG 3 cut(s) 384, 1067, 2079
SstI GAGCTC 1 cut(s) 577
StuI AGGCCT 1 cut(s) 2397
StyD4I CCNGG 7 cut(s) 52, 218, 1267, 1710, 2424, 2611, 2635
StyI CCWWGG 3 cut(s) 156, 459, 2166
TaaI ACNGT 5 cut(s) 961, 1906, 2153, 2504, 2683
TaiI ACGT 2 cut(s) 1553, 2262
TaqI TCGA 9 cut(s) 60, 577, 658, 776, 1251, 1347, 2095, 2243, 2297
TaqII GACCGA 1 cut(s) 755
TatI WGTACW 5 cut(s) 1906, 2160, 2442, 2504, 2593
TfiI GAWTC 6 cut(s) 224, 352, 1371, 1565, 2092, 2585
TscAI CASTG 6 cut(s) 132, 987, 1096, 1885, 1936, 2688
TseFI GTSAC 3 cut(s) 437, 1034, 2260
TseI GCWGC 9 cut(s) 248, 262, 464, 1223, 1394, 1918, 2102, 2627, 2630
Tsp45I GTSAC 3 cut(s) 437, 1034, 2260
TspGWI ACGGA 1 cut(s) 533
TspRI CASTG 6 cut(s) 132, 987, 1096, 1885, 1936, 2688
Van91I CCANNNNNTGG 1 cut(s) 568
VpaK11BI GGWCC 7 cut(s) 302, 676, 699, 1733, 2174, 2220, 2366
XagI CCTNNNNNAGG 1 cut(s) 2616
XbaI TCTAGA 1 cut(s) 2078
XceI RCATGY 5 cut(s) 188, 332, 2242, 2449, 2600
XcmI CCANNNNNNNNNTGG 1 cut(s) 2164
XhoI CTCGAG 3 cut(s) 576, 657, 1250
XmiI GTMKAC 3 cut(s) 1274, 2297, 2382
XmnI GAANNNNTTC 3 cut(s) 498, 893, 2052
XspI CTAG 3 cut(s) 384, 1067, 2079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.