RchiOBHm_Chr4g0404081

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001930
Physical Location & Seq
Forward (+)
23586224 .. 23589471
3248 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37570

Sequence Viewer

Length: 2745 bp
ATGGCAAACCAGAAGCATTTCCTCTGGTTCATCTTCATTGTTTTGTTCATCCCTGGGATCGAGCTTTCAACGACGAAAGAGGTGATACAAGTACCAGTAGGGGTTGTTCTTAACTTAAATTCCTCAGTGGGAGCTATGGCAGAGAACTGCATGACCATGGCACTTGATGATTTTTATACTAAGCATGCTCATTATCAAACAAGGCTTTTCTTTCTCACCAGGGATTCAAAGAATGATGTTGTTTCAGCAGCATTTGAAGCACAGCACTTGATAAACATTGAAAATGTGAGTGCCATTATCGGACCTCAAAGTTCAGCAGAAGCAAAGTTTGTGATAGAGCTGGGAAGAAGGAATCATGTTCCCATTCTTTCGTTTTCTGCCACTAGTCCATCTCTTTCTCCATCCCATAGTTCATTTTTCGTTCGGACAGCTTTTAGTGACTCTGCTCAAGTAGAAGCCATAGCTGCCATTGTCGGAGCTTATAGATGGTTGGAAGTTGTTCTCATCTATGAAGACACCGAGTATGGAAATAGCTTAATTCCATATTTGGTAGACGCTTTCCAGCAAGTTGGCGCTCGAGTACCTTATAGAAGTGTCATTCCTCCTCATTATAATGGCACAAAAATTTTAAAGGAGCTTGACCACTTGAAATCAACTCGAGCAAGAATATTTTTGGTCCATATGACTGCTTCCCTTGGGTCCAAATTCTTTTTCTTAGCAAGTAAGGCAGGGATGATGACTGAAGGGTATGGATGGATTGTTACGGAAGGGTTATCGACTTTATTAGATCCTGTGGGTTCAAGAACCATGGACTCAATGCAAGGTGTAGTGGGGGTAAGGCCATATATAACAATGTCAAAAGAACTTAAAGACTTCGTGTTGCGAATGAAAAGATTCAGGTTCAGAAAATCAAGCAAGATTACTGGTATAAACCTCTTTGGGTTGTGGGCATATGATACAGTTTGGGCATTAGCAATGGCAGTGGAGAAGGTTGGAATAGCAAATTACAGTACCATGATACAAAATGTTAGCACAAGCAGAGTTGATCTTGCAAGTTTGGGAACTGGTGACAGGGGTCAAAAACTTCTTGAAGCTATCCTAGATTTAAAATTTGAAAACCTCAGTGGGAATTTCCGGTTGGCTAAGGGACAGTTGGAAGCTTCAACCTTTGAGGTATTTAATGTGATTGGCGGAAAAGAAAGAATAATTGGATATTGGAACCAAATGAAAGGACTTTCCCGAAAGCTGAATGGAGCAGCAAAGTTGGATGTAAGGAAGACACTCGAGAAACCAATCTGGCCAGGGTACACTACAGACATCCCTCCAACAAAGAAATTAAGGATTGGAGTTCCCTTGAAAAAAGGTTTTTATGAATTCCTAAAATTCGAAAACAACAATATATCAGGATTCTCTGCTGAGATATTCTTTGCTGCAGAAAAGAAACTGCCATTTTCCCTTTCATATGAGTTTTCTGCCTTCGCGGGGACTTATGATGATCTTCTTTATCAAATTCATCTTCAGAAATATGATGCTGTGGTGGGAGACACAACAATTGTAGCTGATCGCTCATTATATGTAGATTTTACGTTGCCTTATTCTGAATCAGGAGTTTCCATGATAGTTTTGACAGAAGATAGTGAGAGGAATAATCTTTGGATTTTCTTGAAGCCACTAAGCTTGGGTCTTTGGTTGACAACCGGCGTAGCCTTCATATTTACAGGTTTTGTAATATGGCTTCTTGAGCACCGGGAAAACACCGAGTTCAGAGGTCCTCCACAGAAACAACTTGGCATGATATTCTGGTTCTCCTTTTCAACCCTCGTCTTTGCACACAGAGAGAAAGTGGTGAACAATTGGTCAAGGTTTGTGCTGATTATATGGGTTTTTGTGGTACTGATCCTAACACAGAGTTACACTGCAAGCTTAGCCTCGATGTTAACAGTACGGAAGTTGCAGCCTGCATTCACTGATATAAAAGAGATACAAAGAAATGGTTACAAAGTAGGATATCAAAATAGTTCCTTTATTAAAGGGTTTCTCATGGAACATTTAAATTTTGATGAGACCCAGTTGGTGCCATTGGTAACCATTCAGGATTATCATAACGCACTCTCTAGAGGAAATAAGAATAGAGTTCCTGCCATTTTAGATGAAGTCCCCTACCTCAAGTTGATCCTTAAAAAGAACTGTTCCAAGTACACCATGGTTGGACCAACCTACAAAACTGATGGATTTGGCTTTGCTTTCCAATCAGGGTCCTCGCTGGTATCCTACATGTCAAGCGCAATCTTGAACGTGACTCAAGATAAAAATCAGATGGATAAACTTCAGTCGACTTACTTTGATTACTTTGATAACCAACCTGCCTGTGAGGATCAAAGTGCCAAAATTTCTTCAGAGGGTCCAAGTCTTGGTGTCTACAGCTTTGGAGGCCTCTTCATCATCGCAGGTGTGGCCTCCATGGTTGCTCTTTTGATGTACATGTATCACTTCCTTTGCTCTCATTGGCCAGCTTTGCGAACAATTAGTTCTTCTGAGAGCAGATTTTGGGAGAAATTGGTTGAAGTGGCAAAGCATTTTGACAAGAAAGATCTCACTTCACATCAGTTTAAGCGAAGAGAATCAAGAGTACATGCTTTAGACACTCCTGATGAAGGTCTAGCAGCTGCACCGGCTGCTAATGATATGCAAAACAACTCATGCAGCAGGAACTCACATAATAGTGGAGAACAATCTTGGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

914

Amino Acids

102.98

Weight (kDa)

8.79

Isoelectric Point (pI)

35.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 40 - 349 1.3e-21 Periplasmic binding protein
ANF_receptor PF01094 48 - 397 2.8e-76 Receptor family ligand binding region
SBP_bac_3 PF00497 457 - 782 3.2e-10 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 560 - 819 3.8e-36 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 612
AarI CACCTGC 1 cut(s) 2438
Acc36I ACCTGC 2 cut(s) 2371, 2438
AccB1I GGYRCC 1 cut(s) 2074
AccB7I CCANNNNNTGG 1 cut(s) 2411
AccI GTMKAC 3 cut(s) 552, 2333, 2418
AccII CGCG 1 cut(s) 1481
AciI CCGC 2 cut(s) 1191, 1481
AclWI GGATC 5 cut(s) 65, 782, 1891, 2167, 2382
AcoI YGGCCR 2 cut(s) 1298, 2507
AcuI CTGAAG 4 cut(s) 762, 1502, 2312, 2379
AfaI GTAC 9 cut(s) 93, 582, 1012, 1307, 1893, 1944, 2198, 2480, 2631
AfiI CCNNNNNNNGG 3 cut(s) 1482, 2411, 2654
AflIII ACRYGT 2 cut(s) 2274, 2481
AhlI ACTAGT 1 cut(s) 383
AjnI CCWGG 3 cut(s) 52, 218, 1300
AjuI GAANNNNNNNTTGG 6 cut(s) 695, 727, 1121, 1153, 1191, 1223
Alw21I GWGCWC 1 cut(s) 1746
Alw26I GTCTC 2 cut(s) 1536, 2057
AlwI GGATC 5 cut(s) 65, 782, 1891, 2167, 2382
Ama87I CYCGRG 3 cut(s) 576, 657, 1283
AoxI GGCC 5 cut(s) 839, 1298, 2431, 2454, 2507
ApeKI GCWGC 9 cut(s) 248, 464, 1256, 1430, 1954, 2663, 2666, 2675, 2703
Asp700I GAANNNNTTC 3 cut(s) 17, 498, 893
AspLEI GCGC 2 cut(s) 575, 2285
AspS9I GGNCC 7 cut(s) 302, 676, 699, 1769, 2210, 2256, 2402
AsuC2I CCSGG 1 cut(s) 1748
AsuHPI GGTGA 4 cut(s) 94, 208, 1079, 1858
AsuII TTCGAA 1 cut(s) 1386
AvaI CYCGRG 3 cut(s) 576, 657, 1283
AvaII GGWCC 7 cut(s) 302, 676, 699, 1769, 2210, 2256, 2402
BalI TGGCCA 2 cut(s) 1300, 2509
BanI GGYRCC 1 cut(s) 2074
BarI GAAGNNNNNNTAC 2 cut(s) 1719, 1751
BbsI GAAGAC 2 cut(s) 519, 1283
Bbv12I GWGCWC 1 cut(s) 1746
BbvI GCAGC 9 cut(s) 260, 451, 1268, 1417, 1966, 2653, 2662, 2675, 2715
BccI CCATC 6 cut(s) 397, 409, 480, 747, 2222, 2311
BciT130I CCWGG 3 cut(s) 54, 220, 1302
BciVI GTATCC 1 cut(s) 2278
BcnI CCSGG 1 cut(s) 1748
BcoDI GTCTC 2 cut(s) 1536, 2057
BcuI ACTAGT 1 cut(s) 383
BfaI CTAG 4 cut(s) 384, 1100, 2115, 2660
BfmI CTRYAG 3 cut(s) 1311, 1431, 2419
BfoI RGCGCY 1 cut(s) 576
BfuAI ACCTGC 2 cut(s) 2371, 2438
BfuI GTATCC 1 cut(s) 2278
BglII AGATCT 1 cut(s) 2590
BisI GCNGC 9 cut(s) 249, 465, 1257, 1431, 1955, 2664, 2667, 2676, 2704
BlpI GCTNAGC 1 cut(s) 1924
BlsI GCNGC 9 cut(s) 250, 466, 1258, 1432, 1956, 2665, 2668, 2677, 2705
Bme1390I CCNGG 4 cut(s) 54, 220, 1302, 1748
Bme18I GGWCC 7 cut(s) 302, 676, 699, 1769, 2210, 2256, 2402
BmeT110I CYCGRG 3 cut(s) 576, 657, 1283
BmgT120I GGNCC 7 cut(s) 302, 676, 699, 1769, 2210, 2256, 2402
BmiI GGNNCC 5 cut(s) 700, 1220, 2076, 2257, 2403
BmrFI CCNGG 4 cut(s) 54, 220, 1302, 1748
BmrI ACTGGG 1 cut(s) 2062
BmsI GCATC 1 cut(s) 1519
BmuI ACTGGG 1 cut(s) 2062
BoxI GACNNNNGTC 1 cut(s) 1074
BpiI GAAGAC 2 cut(s) 519, 1283
Bpu10I CCTNAGC 1 cut(s) 1143
Bpu1102I GCTNAGC 1 cut(s) 1924
Bpu14I TTCGAA 1 cut(s) 1386
BpuEI CTTGAG 4 cut(s) 432, 1760, 2150, 2286
BpuMI CCSGG 1 cut(s) 1748
BsaBI GATNNNNATC 1 cut(s) 2310
BsaI GGTCTC 1 cut(s) 2057
BsaJI CCNNGG 9 cut(s) 52, 53, 156, 219, 694, 807, 1301, 2202, 2460
BsaWI WCCGGW 1 cut(s) 1134
Bsc4I CCNNNNNNNGG 3 cut(s) 1482, 2411, 2654
Bse118I RCCGGY 2 cut(s) 1697, 2671
Bse1I ACTGG 4 cut(s) 95, 928, 1069, 2068
Bse3DI GCAATG 1 cut(s) 981
Bse8I GATNNNNATC 1 cut(s) 2310
BseBI CCWGG 3 cut(s) 54, 220, 1302
BseDI CCNNGG 9 cut(s) 52, 53, 156, 219, 694, 807, 1301, 2202, 2460
BseGI GGATG 6 cut(s) 48, 401, 738, 758, 1273, 1317
BseJI GATNNNNATC 1 cut(s) 2310
BseLI CCNNNNNNNGG 3 cut(s) 1482, 2411, 2654
BseMI GCAATG 1 cut(s) 981
BseMII CTCAG 4 cut(s) 138, 1135, 1407, 2526
BseNI ACTGG 4 cut(s) 95, 928, 1069, 2068
BseRI GAGGAG 1 cut(s) 594
BseXI GCAGC 9 cut(s) 260, 451, 1268, 1417, 1966, 2653, 2662, 2675, 2715
BseYI CCCAGC 1 cut(s) 340
BsgI GTGCAG 1 cut(s) 2652
Bsh1236I CGCG 1 cut(s) 1481
BshFI GGCC 5 cut(s) 841, 1300, 2433, 2456, 2509
BshNI GGYRCC 1 cut(s) 2074
BsiHKAI GWGCWC 1 cut(s) 1746
BsiHKCI CYCGRG 3 cut(s) 576, 657, 1283
BsiSI CCGG 4 cut(s) 1135, 1698, 1747, 2672
BslFI GGGAC 3 cut(s) 1161, 1498, 2141
BslI CCNNNNNNNGG 3 cut(s) 1482, 2411, 2654
BsmAI GTCTC 2 cut(s) 1536, 2057
BsmFI GGGAC 3 cut(s) 1161, 1498, 2141
BsmI GAATGC 1 cut(s) 1961
BsnI GGCC 5 cut(s) 841, 1300, 2433, 2456, 2509
Bso31I GGTCTC 1 cut(s) 2057
BsoBI CYCGRG 3 cut(s) 576, 657, 1283
Bsp119I TTCGAA 1 cut(s) 1386
Bsp1286I GDGCHC 1 cut(s) 1746
Bsp1407I TGTACA 1 cut(s) 2478
Bsp143I GATC 9 cut(s) 57, 787, 1045, 1495, 1561, 1896, 2172, 2374, 2590
Bsp1720I GCTNAGC 1 cut(s) 1924
Bsp19I CCATGG 4 cut(s) 156, 807, 2202, 2460
BspACI CCGC 2 cut(s) 1191, 1481
BspANI GGCC 5 cut(s) 841, 1300, 2433, 2456, 2509
BspCNI CTCAG 4 cut(s) 137, 1134, 1408, 2527
BspFNI CGCG 1 cut(s) 1481
BspLI GGNNCC 5 cut(s) 700, 1220, 2076, 2257, 2403
BspMAI CTGCAG 1 cut(s) 1435
BspMI ACCTGC 2 cut(s) 2371, 2438
BspPI GGATC 5 cut(s) 65, 782, 1891, 2167, 2382
BspT104I TTCGAA 1 cut(s) 1386
BspT107I GGYRCC 1 cut(s) 2074
BspTNI GGTCTC 1 cut(s) 2057
BsrDI GCAATG 1 cut(s) 981
BsrFI RCCGGY 2 cut(s) 1697, 2671
BsrGI TGTACA 1 cut(s) 2478
BsrI ACTGG 4 cut(s) 95, 928, 1069, 2068
BssAI RCCGGY 2 cut(s) 1697, 2671
BssECI CCNNGG 9 cut(s) 52, 53, 156, 219, 694, 807, 1301, 2202, 2460
BssMI GATC 9 cut(s) 57, 787, 1045, 1495, 1561, 1896, 2172, 2374, 2590
BssT1I CCWWGG 5 cut(s) 156, 694, 807, 2202, 2460
Bst2UI CCWGG 3 cut(s) 54, 220, 1302
Bst4CI ACNGT 5 cut(s) 961, 1010, 1152, 1942, 2189
Bst6I CTCTTC 2 cut(s) 2441, 2611
BstAPI GCANNNNNTGC 1 cut(s) 2675
BstAUI TGTACA 1 cut(s) 2478
BstBI TTCGAA 1 cut(s) 1386
BstC8I GCNNGC 4 cut(s) 186, 1921, 1959, 2511
BstDEI CTNAG 9 cut(s) 124, 180, 715, 1121, 1143, 1416, 1673, 1924, 2535
BstDSI CCRYGG 4 cut(s) 156, 807, 2202, 2460
BstEII GGTNACC 1 cut(s) 2083
BstENI CCTNNNNNAGG 1 cut(s) 2652
BstF5I GGATG 6 cut(s) 48, 401, 738, 758, 1273, 1317
BstFNI CGCG 1 cut(s) 1481
BstH2I RGCGCY 1 cut(s) 576
BstHHI GCGC 2 cut(s) 575, 2285
BstKTI GATC 9 cut(s) 60, 790, 1048, 1498, 1564, 1899, 2175, 2377, 2593
BstMAI GTCTC 2 cut(s) 1536, 2057
BstMBI GATC 9 cut(s) 57, 787, 1045, 1495, 1561, 1896, 2172, 2374, 2590
BstNI CCWGG 3 cut(s) 54, 220, 1302
BstNSI RCATGY 4 cut(s) 188, 2278, 2485, 2636
BstPAI GACNNNNGTC 1 cut(s) 1074
BstPI GGTNACC 1 cut(s) 2083
BstSCI CCNGG 4 cut(s) 52, 218, 1300, 1746
BstSFI CTRYAG 3 cut(s) 1311, 1431, 2419
BstUI CGCG 1 cut(s) 1481
BstV1I GCAGC 9 cut(s) 260, 451, 1268, 1417, 1966, 2653, 2662, 2675, 2715
BstV2I GAAGAC 2 cut(s) 519, 1283
BstX2I RGATCY 2 cut(s) 787, 2590
BstXI CCANNNNNNTGG 1 cut(s) 569
BstYI RGATCY 2 cut(s) 787, 2590
BsuI GTATCC 1 cut(s) 2278
BsuRI GGCC 5 cut(s) 841, 1300, 2433, 2456, 2509
BtgI CCRYGG 4 cut(s) 156, 807, 2202, 2460
BtgZI GCGATG 1 cut(s) 2428
BtsCI GGATG 6 cut(s) 48, 401, 738, 758, 1273, 1317
BtsI GCAGTG 2 cut(s) 987, 1914
BtsIMutI CAGTG 5 cut(s) 132, 987, 1129, 1914, 1965
BveI ACCTGC 2 cut(s) 2371, 2438
Cac8I GCNNGC 4 cut(s) 186, 1921, 1959, 2511
CfoI GCGC 2 cut(s) 575, 2285
Cfr10I RCCGGY 2 cut(s) 1697, 2671
Cfr13I GGNCC 7 cut(s) 302, 676, 699, 1769, 2210, 2256, 2402
CseI GACGC 1 cut(s) 563
Csp6I GTAC 9 cut(s) 92, 581, 1011, 1306, 1892, 1943, 2197, 2479, 2630
CspCI CAANNNNNGTGG 4 cut(s) 963, 998, 1659, 1694
CviQI GTAC 9 cut(s) 92, 581, 1011, 1306, 1892, 1943, 2197, 2479, 2630
DdeI CTNAG 9 cut(s) 124, 180, 715, 1121, 1143, 1416, 1673, 1924, 2535
DpnI GATC 9 cut(s) 59, 789, 1047, 1497, 1563, 1898, 2174, 2376, 2592
DpnII GATC 9 cut(s) 57, 787, 1045, 1495, 1561, 1896, 2172, 2374, 2590
DraI TTTAAA 3 cut(s) 630, 1107, 2052
EaeI YGGCCR 2 cut(s) 1298, 2507
Eam1104I CTCTTC 2 cut(s) 2441, 2611
EarI CTCTTC 2 cut(s) 2441, 2611
EciI GGCGGA 1 cut(s) 1206
Eco130I CCWWGG 5 cut(s) 156, 694, 807, 2202, 2460
Eco147I AGGCCT 1 cut(s) 2433
Eco31I GGTCTC 1 cut(s) 2057
Eco32I GATATC 1 cut(s) 2009
Eco47I GGWCC 7 cut(s) 302, 676, 699, 1769, 2210, 2256, 2402
Eco57I CTGAAG 4 cut(s) 762, 1502, 2312, 2379
Eco88I CYCGRG 3 cut(s) 576, 657, 1283
Eco91I GGTNACC 1 cut(s) 2083
EcoNI CCTNNNNNAGG 1 cut(s) 2652
EcoO109I RGGNCCY 2 cut(s) 1769, 2256
EcoO65I GGTNACC 1 cut(s) 2083
EcoRI GAATTC 1 cut(s) 1373
EcoRII CCWGG 3 cut(s) 52, 218, 1300
EcoRV GATATC 1 cut(s) 2009
EcoT14I CCWWGG 5 cut(s) 156, 694, 807, 2202, 2460
ErhI CCWWGG 5 cut(s) 156, 694, 807, 2202, 2460
FaqI GGGAC 3 cut(s) 1161, 1498, 2141
FauI CCCGC 1 cut(s) 1474
FauNDI CATATG 3 cut(s) 681, 952, 1462
FblI GTMKAC 3 cut(s) 552, 2333, 2418
Fnu4HI GCNGC 9 cut(s) 249, 465, 1257, 1431, 1955, 2664, 2667, 2676, 2704
FokI GGATG 6 cut(s) 35, 388, 745, 765, 1280, 1304
Fsp4HI GCNGC 9 cut(s) 249, 465, 1257, 1431, 1955, 2664, 2667, 2676, 2704
FspBI CTAG 4 cut(s) 384, 1100, 2115, 2660
GlaI GCGC 2 cut(s) 574, 2284
GluI GCNGC 9 cut(s) 249, 465, 1257, 1431, 1955, 2664, 2667, 2676, 2704
GsaI CCCAGC 1 cut(s) 344
HaeII RGCGCY 1 cut(s) 576
HaeIII GGCC 5 cut(s) 841, 1300, 2433, 2456, 2509
HapII CCGG 4 cut(s) 1135, 1698, 1747, 2672
HgaI GACGC 1 cut(s) 563
HhaI GCGC 2 cut(s) 575, 2285
Hin6I GCGC 2 cut(s) 573, 2283
HinP1I GCGC 2 cut(s) 573, 2283
HincII GTYRAC 3 cut(s) 1692, 1938, 2334
HindII GTYRAC 3 cut(s) 1692, 1938, 2334
HindIII AAGCTT 3 cut(s) 1158, 1675, 1921
HinfI GANTC 9 cut(s) 224, 352, 440, 812, 894, 1407, 1601, 2299, 2621
HpaI GTTAAC 1 cut(s) 1938
HpaII CCGG 4 cut(s) 1135, 1698, 1747, 2672
HphI GGTGA 4 cut(s) 94, 208, 1079, 1858
Hpy166II GTNNAC 8 cut(s) 553, 1308, 1692, 1849, 1938, 2199, 2334, 2419
Hpy8I GTNNAC 8 cut(s) 553, 1308, 1692, 1849, 1938, 2199, 2334, 2419
Hpy99I CGWCG 1 cut(s) 76
HpyAV CCTTC 7 cut(s) 342, 737, 761, 982, 1486, 1717, 2648
HpyCH4III ACNGT 5 cut(s) 961, 1010, 1152, 1942, 2189
HpyCH4IV ACGT 2 cut(s) 1586, 2295
HpyF3I CTNAG 9 cut(s) 124, 180, 715, 1121, 1143, 1416, 1673, 1924, 2535
HpySE526I ACGT 2 cut(s) 1586, 2295
HspAI GCGC 2 cut(s) 573, 2283
KspAI GTTAAC 1 cut(s) 1938
Kzo9I GATC 9 cut(s) 57, 787, 1045, 1495, 1561, 1896, 2172, 2374, 2590
LmnI GCTCC 4 cut(s) 131, 476, 634, 1253
Lsp1109I GCAGC 9 cut(s) 260, 451, 1268, 1417, 1966, 2653, 2662, 2675, 2715
LweI GCATC 1 cut(s) 1519
MaeI CTAG 4 cut(s) 384, 1100, 2115, 2660
MaeII ACGT 2 cut(s) 1586, 2295
MaeIII GTNAC 7 cut(s) 437, 760, 1067, 1910, 1994, 2083, 2296
MalI GATC 9 cut(s) 59, 789, 1047, 1497, 1563, 1898, 2174, 2376, 2592
MboI GATC 9 cut(s) 57, 787, 1045, 1495, 1561, 1896, 2172, 2374, 2590
MfeI CAATTG 2 cut(s) 1551, 1852
MflI RGATCY 2 cut(s) 787, 2590
MhlI GDGCHC 1 cut(s) 1746
MlsI TGGCCA 2 cut(s) 1300, 2509
MluNI TGGCCA 2 cut(s) 1300, 2509
MlyI GAGTC 3 cut(s) 434, 806, 2293
MmeI TCCRAC 7 cut(s) 454, 471, 973, 1134, 1245, 1349, 2188
Mox20I TGGCCA 2 cut(s) 1300, 2509
MroXI GAANNNNTTC 3 cut(s) 17, 498, 893
MscI TGGCCA 2 cut(s) 1300, 2509
MslI CAYNNNNRTG 3 cut(s) 155, 612, 2721
Msp20I TGGCCA 2 cut(s) 1300, 2509
MspA1I CMGCKG 1 cut(s) 2666
MspI CCGG 4 cut(s) 1135, 1698, 1747, 2672
MspR9I CCNGG 4 cut(s) 54, 220, 1302, 1748
MunI CAATTG 2 cut(s) 1551, 1852
Mva1269I GAATGC 1 cut(s) 1961
MvaI CCWGG 3 cut(s) 54, 220, 1302
MvnI CGCG 1 cut(s) 1481
NciI CCSGG 1 cut(s) 1748
NcoI CCATGG 4 cut(s) 156, 807, 2202, 2460
NdeI CATATG 3 cut(s) 681, 952, 1462
NdeII GATC 9 cut(s) 57, 787, 1045, 1495, 1561, 1896, 2172, 2374, 2590
NlaIV GGNNCC 5 cut(s) 700, 1220, 2076, 2257, 2403
NmuCI GTSAC 3 cut(s) 437, 1067, 2296
NspI RCATGY 4 cut(s) 188, 2278, 2485, 2636
NspV TTCGAA 1 cut(s) 1386
PaeI GCATGC 1 cut(s) 188
PaeR7I CTCGAG 3 cut(s) 576, 657, 1283
PaqCI CACCTGC 1 cut(s) 2438
PasI CCCWGGG 1 cut(s) 53
PceI AGGCCT 1 cut(s) 2433
PciI ACATGT 2 cut(s) 2274, 2481
PctI GAATGC 1 cut(s) 1961
PdmI GAANNNNTTC 3 cut(s) 17, 498, 893
PfeI GAWTC 6 cut(s) 224, 352, 894, 1407, 1601, 2621
PflMI CCANNNNNTGG 1 cut(s) 2411
PkrI GCNGC 9 cut(s) 250, 466, 1258, 1432, 1956, 2665, 2668, 2677, 2705
PleI GAGTC 3 cut(s) 434, 806, 2293
PpsI GAGTC 3 cut(s) 434, 806, 2293
PpuMI RGGWCCY 2 cut(s) 1769, 2256
PscI ACATGT 2 cut(s) 2274, 2481
PshAI GACNNNNGTC 1 cut(s) 1074
PsiI TTATAA 1 cut(s) 612
Psp5II RGGWCCY 2 cut(s) 1769, 2256
Psp6I CCWGG 3 cut(s) 52, 218, 1300
PspEI GGTNACC 1 cut(s) 2083
PspFI CCCAGC 1 cut(s) 340
PspGI CCWGG 3 cut(s) 52, 218, 1300
PspN4I GGNNCC 5 cut(s) 700, 1220, 2076, 2257, 2403
PspPI GGNCC 7 cut(s) 302, 676, 699, 1769, 2210, 2256, 2402
PspPPI RGGWCCY 2 cut(s) 1769, 2256
PspXI VCTCGAGB 2 cut(s) 576, 657
PsrI GAACNNNNNNTAC 2 cut(s) 90, 122
PstI CTGCAG 1 cut(s) 1435
PsuI RGATCY 2 cut(s) 787, 2590
PvuII CAGCTG 1 cut(s) 2666
RsaI GTAC 9 cut(s) 93, 582, 1012, 1307, 1893, 1944, 2198, 2480, 2631
RsaNI GTAC 9 cut(s) 92, 581, 1011, 1306, 1892, 1943, 2197, 2479, 2630
RseI CAYNNNNRTG 3 cut(s) 155, 612, 2721
SalI GTCGAC 1 cut(s) 2332
SatI GCNGC 9 cut(s) 249, 465, 1257, 1431, 1955, 2664, 2667, 2676, 2704
Sau3AI GATC 9 cut(s) 57, 787, 1045, 1495, 1561, 1896, 2172, 2374, 2590
Sau96I GGNCC 7 cut(s) 302, 676, 699, 1769, 2210, 2256, 2402
SchI GAGTC 3 cut(s) 434, 806, 2293
ScrFI CCNGG 4 cut(s) 54, 220, 1302, 1748
SduI GDGCHC 1 cut(s) 1746
SfaNI GCATC 1 cut(s) 1519
SfcI CTRYAG 3 cut(s) 1311, 1431, 2419
Sfr274I CTCGAG 3 cut(s) 576, 657, 1283
SfuI TTCGAA 1 cut(s) 1386
SinI GGWCC 7 cut(s) 302, 676, 699, 1769, 2210, 2256, 2402
SlaI CTCGAG 3 cut(s) 576, 657, 1283
SmiI ATTTAAAT 1 cut(s) 2052
SmiMI CAYNNNNRTG 3 cut(s) 155, 612, 2721
SmlI CTYRAG 7 cut(s) 447, 576, 657, 1283, 1739, 2165, 2301
SmoI CTYRAG 7 cut(s) 447, 576, 657, 1283, 1739, 2165, 2301
SpeI ACTAGT 1 cut(s) 383
SphI GCATGC 1 cut(s) 188
SseBI AGGCCT 1 cut(s) 2433
SsiI CCGC 2 cut(s) 1191, 1481
SspI AATATT 1 cut(s) 669
SspMI CTAG 4 cut(s) 384, 1100, 2115, 2660
StuI AGGCCT 1 cut(s) 2433
StyD4I CCNGG 4 cut(s) 52, 218, 1300, 1746
StyI CCWWGG 5 cut(s) 156, 694, 807, 2202, 2460
SwaI ATTTAAAT 1 cut(s) 2052
TaaI ACNGT 5 cut(s) 961, 1010, 1152, 1942, 2189
TaiI ACGT 2 cut(s) 1589, 2298
TaqI TCGA 8 cut(s) 60, 577, 658, 776, 1284, 1386, 1931, 2333
TatI WGTACW 3 cut(s) 2196, 2478, 2629
TfiI GAWTC 6 cut(s) 224, 352, 894, 1407, 1601, 2621
TscAI CASTG 5 cut(s) 132, 987, 1129, 1921, 1972
TseFI GTSAC 3 cut(s) 437, 1067, 2296
TseI GCWGC 9 cut(s) 248, 464, 1256, 1430, 1954, 2663, 2666, 2675, 2703
Tsp45I GTSAC 3 cut(s) 437, 1067, 2296
TspGWI ACGGA 2 cut(s) 779, 1960
TspRI CASTG 5 cut(s) 132, 987, 1129, 1921, 1972
Van91I CCANNNNNTGG 1 cut(s) 2411
VpaK11BI GGWCC 7 cut(s) 302, 676, 699, 1769, 2210, 2256, 2402
XagI CCTNNNNNAGG 1 cut(s) 2652
XbaI TCTAGA 1 cut(s) 2114
XceI RCATGY 4 cut(s) 188, 2278, 2485, 2636
XcmI CCANNNNNNNNNTGG 1 cut(s) 2200
XhoI CTCGAG 3 cut(s) 576, 657, 1283
XmiI GTMKAC 3 cut(s) 552, 2333, 2418
XmnI GAANNNNTTC 3 cut(s) 17, 498, 893
XspI CTAG 4 cut(s) 384, 1100, 2115, 2660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.