Rroxscaffold_5G00348550

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001930
Physical Location & Seq
Forward (+)
20850717 .. 20854386
3670 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00348550.1

Sequence Viewer

Length: 2343 bp
ATGGTACCTACATATGAGAATGCAAAGACTTCAACAATAATTCCAGGTAGCCCGACCATGGCAAACCAGAAGCGTTTCCTCTGGTTCATCTTCATTGTTTTGTTCATCCCTGGGATCGAGCCTTCAACGACAAAAGAGGTGATACGAGTACCAGTAGGAGTTGTTCTTAACTTAAATTCCTCAGTGGGAGCTATGGCAGAGAACTGCATGACCATGGCACTTGATGATTTTTATGCTAAGCATGCTCATTATCAAACAAGGCTTTTCTTTCTCACCAGGGATTCAAAGAATGATGTTGTTTCAGCAGCATTTGAAGCACAGCACTTGATAAACATTGAAAATGTGAGTGCCATTATCGGACCTCAAAGTTCAGCAGAAGCAAAGTTTGTGATAGAGCTGGGAAGAAGGAATCATGTTCCCATTCTTTCGTTTTCTGCCACTAGTCCATCTCTTTCTCCATCCCATAGTTCTTTTTTTGTTCGGACGGCTTTTACTGACTCTGCTCAGGTAGAAGCCATAGCTGCCATTGTCGGAGCTTATAGATGGTTGGAAGTTGTTCTCATATATGAAGACACCGAGTACGGAAATAGCTTAATTCCATGTTTGGTAGACGCTTTCCAGCAAGTTGGTGCTCGAGTACCTTATAGAAGTGTCATTCCTCCTCATTATAATGGCACAAAAATTTTAAAGGAGCTTAACCACTTGAAATCAACTCGAGCAAGAATATTTTTGGTCCATATGACTGCTTCCCTTGGGTCCAAATTCTTTTTCTTAGCAAGTAAGGCAGGGATGATGACTGAAGGGTATGGATGGATTGTTACGGAAGGGTTATCGACTTTATTAGATCCTGTGGGTTCAAGAACCATGGACTCAATGCAAGGTGTAGTGGGAGTAAGGCCATATATACCAATGTCAAAAGAACTTGAAGACTTCGTGTTGCGACTGAAAAGATTCAGGATCAGAAAATCAAGCAAGATTACTGGTATAAACCTCTTTGGGTTGTGGGCATATGATACAGTTTGGGCATTAGCAATGGCAGTGGAGAAGGTTGGAATAGCAAATTATAGGACCCTGATACAAAATGTTAGCACAAGCAGAGTTGATCTTGCAAGTTTGGGAACTGGTGACAGGGGTCAAAATCTTCTTGAAGCTATCCTAGATTTAAAATTTGAAAACCTCAGTGGGAATTTCCGGTTGGCTAACGGACAGTTGGAAGCTTCAACCTTTGAGGTATTTAATGTGATTGGCGGAAAAGAAAGAATAATTGGATATTGGAACCAAAAGAAAGGACTTTCCCGAAAGCTGAATGGAGCAGCAGAGTTGGATAAAAGGAAGACACTCGAGAAACCAATCTGGCCAGGGTACACTACAGACATCCCTCCAACAAAGAAATTGAGGATTGGAGTTCCCTTGAAACAGGGTTTTTATGAATTCCTAAAATTCGAAAACAACAATATATCAGGATTCTCTGCTGAGATATTCTTTGCTGCAGAAAAGAAACTGCCATTTTCCCTTTCATATGAGTTTTCTGCCTTCGCGGGGACTTATGATGATCTTCTTTATCAAATTCATCTTCAGAAATACGACGCTGTGGTGGGAGACACAACAATTATAGCTAATCGCTCATTATATGTAGATTTTACGTTGCCTTATTCTGAATCAGGAGTTTCCATGATAGTTTTGACAGAAGATAGTGAGAGGAATAGTCTTTGGATTTTCTTGAAGCCACTAAGCTTGGGTCTTTGGCTGACAACCGGCATAGCTTTCATATTTACAGGTTTTGTAATATGGCTTCTTGAGCACCGGGAAAACACCGAGTTCAGAGGTCCTCCACAGCAACAACTTGGCATGATATTCTGGTTCTCCTTTTCAACCCTCGTCTTTGCACACCGAGAGAAAGTGGTGAACAATTGGTCAAGGTTTGTGCTGATTATATGGGTTTTTGTGGTACTGATCCTAACACAGAGTTACACTGCAAGCTTAGCCTCAATGTTAACAGTACAGAAGTTGCAGCCAGCATTCACTGATATAAAAGAGATACAAAGAAATGGTTACAAAGTAGGATATCAAAATAGTTCCTTTATTAAAGGGTTTCTCATGGAACATTTAAATTTTGATGAGACCCAGTTGGTGCCATTGGTAACCATTCAGGATTACCATAACGCACTCTCTAGAGGAAATAAGAATAGAGTTGCTGCCATTTTAGATGAAGTCCCTTACCTCAAGTTGATCCTTAAAAAGAACTGTTCCAAGTACACCATGGTTGGACCAACCTACAAAACTGATGGATTTGGCTTTGGTCCTCGCTGGTATCCTACTTGTCAAGCGCAATCTTGGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

780

Amino Acids

87.91

Weight (kDa)

9.08

Isoelectric Point (pI)

31.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 60 - 368 1.2e-21 Periplasmic binding protein
ANF_receptor PF01094 67 - 416 8.4e-77 Receptor family ligand binding region
SBP_bac_3 PF00497 476 - 600 6.5e-09 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 579 - 750 1.2e-31 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 669
Acc65I GGTACC 1 cut(s) 4
AccB1I GGYRCC 2 cut(s) 4, 2131
AccI GTMKAC 1 cut(s) 609
AccII CGCG 1 cut(s) 1538
AciI CCGC 2 cut(s) 1248, 1538
AclWI GGATC 5 cut(s) 122, 839, 965, 1948, 2224
AcoI YGGCCR 1 cut(s) 1355
AcsI RAATTY 9 cut(s) 175, 681, 761, 1166, 1186, 1430, 1439, 1566, 2110
AcuI CTGAAG 2 cut(s) 819, 1559
AfaI GTAC 8 cut(s) 6, 150, 581, 639, 1364, 1950, 2001, 2255
AfiI CCNNNNNNNGG 2 cut(s) 58, 1539
AhlI ACTAGT 1 cut(s) 440
AjiI CACGTC 1 cut(s) 2340
AjnI CCWGG 4 cut(s) 43, 109, 275, 1357
AjuI GAANNNNNNNTTGG 6 cut(s) 752, 784, 1178, 1210, 1248, 1280
Alw21I GWGCWC 2 cut(s) 634, 1803
Alw26I GTCTC 2 cut(s) 1593, 2114
AlwI GGATC 5 cut(s) 122, 839, 965, 1948, 2224
Ama87I CYCGRG 3 cut(s) 633, 714, 1340
AoxI GGCC 2 cut(s) 896, 1355
ApeKI GCWGC 6 cut(s) 305, 521, 1313, 1487, 2011, 2195
ApoI RAATTY 9 cut(s) 175, 681, 761, 1166, 1186, 1430, 1439, 1566, 2110
Asp700I GAANNNNTTC 3 cut(s) 74, 555, 950
Asp718I GGTACC 1 cut(s) 4
AspLEI GCGC 1 cut(s) 2329
AspS9I GGNCC 7 cut(s) 359, 733, 756, 1068, 1826, 2267, 2300
AsuC2I CCSGG 1 cut(s) 1805
AsuHPI GGTGA 4 cut(s) 151, 265, 1136, 1915
AsuII TTCGAA 1 cut(s) 1443
AvaI CYCGRG 3 cut(s) 633, 714, 1340
AvaII GGWCC 7 cut(s) 359, 733, 756, 1068, 1826, 2267, 2300
BalI TGGCCA 1 cut(s) 1357
BanI GGYRCC 2 cut(s) 4, 2131
BarI GAAGNNNNNNTAC 2 cut(s) 1776, 1808
BbsI GAAGAC 3 cut(s) 576, 933, 1340
Bbv12I GWGCWC 2 cut(s) 634, 1803
BbvI GCAGC 6 cut(s) 317, 508, 1325, 1474, 2023, 2182
BccI CCATC 5 cut(s) 454, 466, 537, 804, 2279
BceAI ACGGC 1 cut(s) 501
BciT130I CCWGG 4 cut(s) 45, 111, 277, 1359
BciVI GTATCC 1 cut(s) 2322
BcnI CCSGG 1 cut(s) 1805
BcoDI GTCTC 2 cut(s) 1593, 2114
BcuI ACTAGT 1 cut(s) 440
BfaI CTAG 3 cut(s) 441, 1157, 2172
BfmI CTRYAG 2 cut(s) 1368, 1488
BfuI GTATCC 1 cut(s) 2322
BisI GCNGC 6 cut(s) 306, 522, 1314, 1488, 2012, 2196
BlpI GCTNAGC 2 cut(s) 237, 1981
BlsI GCNGC 6 cut(s) 307, 523, 1315, 1489, 2013, 2197
Bme1390I CCNGG 5 cut(s) 45, 111, 277, 1359, 1805
Bme18I GGWCC 7 cut(s) 359, 733, 756, 1068, 1826, 2267, 2300
BmeT110I CYCGRG 3 cut(s) 633, 714, 1340
BmgBI CACGTC 1 cut(s) 2340
BmgT120I GGNCC 7 cut(s) 359, 733, 756, 1068, 1826, 2267, 2300
BmiI GGNNCC 5 cut(s) 6, 757, 1070, 1277, 2133
BmrFI CCNGG 5 cut(s) 45, 111, 277, 1359, 1805
BmrI ACTGGG 1 cut(s) 2119
BmuI ACTGGG 1 cut(s) 2119
BoxI GACNNNNGTC 1 cut(s) 1131
BpiI GAAGAC 3 cut(s) 576, 933, 1340
Bpu10I CCTNAGC 1 cut(s) 504
Bpu1102I GCTNAGC 2 cut(s) 237, 1981
Bpu14I TTCGAA 1 cut(s) 1443
BpuEI CTTGAG 2 cut(s) 1817, 2207
BpuMI CCSGG 1 cut(s) 1805
BsaI GGTCTC 1 cut(s) 2114
BsaJI CCNNGG 9 cut(s) 57, 109, 110, 213, 276, 751, 864, 1358, 2259
BsaWI WCCGGW 1 cut(s) 1191
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 1539
Bse118I RCCGGY 1 cut(s) 1754
Bse1I ACTGG 4 cut(s) 152, 985, 1126, 2125
Bse3DI GCAATG 1 cut(s) 1038
BseBI CCWGG 4 cut(s) 45, 111, 277, 1359
BseDI CCNNGG 9 cut(s) 57, 109, 110, 213, 276, 751, 864, 1358, 2259
BseGI GGATG 5 cut(s) 105, 458, 795, 815, 1374
BseLI CCNNNNNNNGG 2 cut(s) 58, 1539
BseMI GCAATG 1 cut(s) 1038
BseMII CTCAG 4 cut(s) 195, 518, 1192, 1464
BseNI ACTGG 4 cut(s) 152, 985, 1126, 2125
BseRI GAGGAG 1 cut(s) 651
BseXI GCAGC 6 cut(s) 317, 508, 1325, 1474, 2023, 2182
BseYI CCCAGC 1 cut(s) 397
Bsh1236I CGCG 1 cut(s) 1538
BshFI GGCC 2 cut(s) 898, 1357
BshNI GGYRCC 2 cut(s) 4, 2131
BsiHKAI GWGCWC 2 cut(s) 634, 1803
BsiHKCI CYCGRG 3 cut(s) 633, 714, 1340
BsiSI CCGG 3 cut(s) 1192, 1755, 1804
BslFI GGGAC 2 cut(s) 1555, 2198
BslI CCNNNNNNNGG 2 cut(s) 58, 1539
BsmAI GTCTC 2 cut(s) 1593, 2114
BsmFI GGGAC 2 cut(s) 1555, 2198
BsmI GAATGC 2 cut(s) 25, 2018
BsnI GGCC 2 cut(s) 898, 1357
Bso31I GGTCTC 1 cut(s) 2114
BsoBI CYCGRG 3 cut(s) 633, 714, 1340
Bsp119I TTCGAA 1 cut(s) 1443
Bsp1286I GDGCHC 2 cut(s) 634, 1803
Bsp143I GATC 7 cut(s) 114, 844, 957, 1102, 1552, 1953, 2229
Bsp1720I GCTNAGC 2 cut(s) 237, 1981
Bsp19I CCATGG 4 cut(s) 57, 213, 864, 2259
BspACI CCGC 2 cut(s) 1248, 1538
BspANI GGCC 2 cut(s) 898, 1357
BspCNI CTCAG 4 cut(s) 194, 517, 1191, 1465
BspFNI CGCG 1 cut(s) 1538
BspLI GGNNCC 5 cut(s) 6, 757, 1070, 1277, 2133
BspMAI CTGCAG 1 cut(s) 1492
BspPI GGATC 5 cut(s) 122, 839, 965, 1948, 2224
BspT104I TTCGAA 1 cut(s) 1443
BspT107I GGYRCC 2 cut(s) 4, 2131
BspTNI GGTCTC 1 cut(s) 2114
BsrDI GCAATG 1 cut(s) 1038
BsrFI RCCGGY 1 cut(s) 1754
BsrI ACTGG 4 cut(s) 152, 985, 1126, 2125
BssAI RCCGGY 1 cut(s) 1754
BssECI CCNNGG 9 cut(s) 57, 109, 110, 213, 276, 751, 864, 1358, 2259
BssMI GATC 7 cut(s) 114, 844, 957, 1102, 1552, 1953, 2229
BssT1I CCWWGG 5 cut(s) 57, 213, 751, 864, 2259
Bst2UI CCWGG 4 cut(s) 45, 111, 277, 1359
Bst4CI ACNGT 4 cut(s) 1018, 1209, 1999, 2246
BstBI TTCGAA 1 cut(s) 1443
BstC8I GCNNGC 3 cut(s) 243, 1978, 2016
BstDEI CTNAG 8 cut(s) 181, 237, 504, 772, 1178, 1473, 1730, 1981
BstDSI CCRYGG 4 cut(s) 57, 213, 864, 2259
BstEII GGTNACC 1 cut(s) 2140
BstF5I GGATG 5 cut(s) 105, 458, 795, 815, 1374
BstFNI CGCG 1 cut(s) 1538
BstHHI GCGC 1 cut(s) 2329
BstKTI GATC 7 cut(s) 117, 847, 960, 1105, 1555, 1956, 2232
BstMAI GTCTC 2 cut(s) 1593, 2114
BstMBI GATC 7 cut(s) 114, 844, 957, 1102, 1552, 1953, 2229
BstMWI GCNNNNNNNGC 6 cut(s) 242, 314, 521, 782, 1798, 1982
BstNI CCWGG 4 cut(s) 45, 111, 277, 1359
BstNSI RCATGY 1 cut(s) 245
BstPAI GACNNNNGTC 1 cut(s) 1131
BstPI GGTNACC 1 cut(s) 2140
BstSCI CCNGG 5 cut(s) 43, 109, 275, 1357, 1803
BstSFI CTRYAG 2 cut(s) 1368, 1488
BstUI CGCG 1 cut(s) 1538
BstV1I GCAGC 6 cut(s) 317, 508, 1325, 1474, 2023, 2182
BstV2I GAAGAC 3 cut(s) 576, 933, 1340
BstX2I RGATCY 1 cut(s) 844
BstXI CCANNNNNNTGG 1 cut(s) 626
BstYI RGATCY 1 cut(s) 844
BsuI GTATCC 1 cut(s) 2322
BsuRI GGCC 2 cut(s) 898, 1357
BtgI CCRYGG 4 cut(s) 57, 213, 864, 2259
BtrI CACGTC 1 cut(s) 2340
BtsCI GGATG 5 cut(s) 105, 458, 795, 815, 1374
BtsI GCAGTG 2 cut(s) 1044, 1971
BtsIMutI CAGTG 5 cut(s) 189, 1044, 1186, 1971, 2022
Cac8I GCNNGC 3 cut(s) 243, 1978, 2016
CfoI GCGC 1 cut(s) 2329
Cfr10I RCCGGY 1 cut(s) 1754
Cfr13I GGNCC 7 cut(s) 359, 733, 756, 1068, 1826, 2267, 2300
CseI GACGC 2 cut(s) 620, 1595
Csp6I GTAC 8 cut(s) 5, 149, 580, 638, 1363, 1949, 2000, 2254
CspCI CAANNNNNGTGG 4 cut(s) 1020, 1055, 1716, 1751
CviQI GTAC 8 cut(s) 5, 149, 580, 638, 1363, 1949, 2000, 2254
DdeI CTNAG 8 cut(s) 181, 237, 504, 772, 1178, 1473, 1730, 1981
DpnI GATC 7 cut(s) 116, 846, 959, 1104, 1554, 1955, 2231
DpnII GATC 7 cut(s) 114, 844, 957, 1102, 1552, 1953, 2229
DraI TTTAAA 3 cut(s) 687, 1164, 2109
EaeI YGGCCR 1 cut(s) 1355
EciI GGCGGA 1 cut(s) 1263
Eco130I CCWWGG 5 cut(s) 57, 213, 751, 864, 2259
Eco31I GGTCTC 1 cut(s) 2114
Eco32I GATATC 1 cut(s) 2066
Eco47I GGWCC 7 cut(s) 359, 733, 756, 1068, 1826, 2267, 2300
Eco57I CTGAAG 2 cut(s) 819, 1559
Eco88I CYCGRG 3 cut(s) 633, 714, 1340
Eco91I GGTNACC 1 cut(s) 2140
EcoO109I RGGNCCY 2 cut(s) 1068, 1826
EcoO65I GGTNACC 1 cut(s) 2140
EcoRI GAATTC 1 cut(s) 1430
EcoRII CCWGG 4 cut(s) 43, 109, 275, 1357
EcoRV GATATC 1 cut(s) 2066
EcoT14I CCWWGG 5 cut(s) 57, 213, 751, 864, 2259
ErhI CCWWGG 5 cut(s) 57, 213, 751, 864, 2259
FaqI GGGAC 2 cut(s) 1555, 2198
FauI CCCGC 1 cut(s) 1531
FauNDI CATATG 4 cut(s) 13, 738, 1009, 1519
FblI GTMKAC 1 cut(s) 609
Fnu4HI GCNGC 6 cut(s) 306, 522, 1314, 1488, 2012, 2196
FokI GGATG 5 cut(s) 92, 445, 802, 822, 1361
Fsp4HI GCNGC 6 cut(s) 306, 522, 1314, 1488, 2012, 2196
FspBI CTAG 3 cut(s) 441, 1157, 2172
GlaI GCGC 1 cut(s) 2328
GluI GCNGC 6 cut(s) 306, 522, 1314, 1488, 2012, 2196
GsaI CCCAGC 1 cut(s) 401
HaeIII GGCC 2 cut(s) 898, 1357
HapII CCGG 3 cut(s) 1192, 1755, 1804
HgaI GACGC 2 cut(s) 620, 1595
HhaI GCGC 1 cut(s) 2329
Hin6I GCGC 1 cut(s) 2327
HinP1I GCGC 1 cut(s) 2327
HincII GTYRAC 1 cut(s) 1995
HindII GTYRAC 1 cut(s) 1995
HindIII AAGCTT 3 cut(s) 1215, 1732, 1978
HinfI GANTC 7 cut(s) 281, 409, 497, 869, 951, 1464, 1658
HpaI GTTAAC 1 cut(s) 1995
HpaII CCGG 3 cut(s) 1192, 1755, 1804
HphI GGTGA 4 cut(s) 151, 265, 1136, 1915
Hpy166II GTNNAC 5 cut(s) 610, 1365, 1906, 1995, 2256
Hpy188I TCNGA 7 cut(s) 359, 483, 533, 962, 1578, 1657, 1823
Hpy8I GTNNAC 5 cut(s) 610, 1365, 1906, 1995, 2256
Hpy99I CGWCG 1 cut(s) 1589
HpyAV CCTTC 6 cut(s) 132, 399, 794, 818, 1039, 1543
HpyCH4III ACNGT 4 cut(s) 1018, 1209, 1999, 2246
HpyCH4IV ACGT 2 cut(s) 1643, 2339
HpyCH4V TGCA 8 cut(s) 23, 207, 877, 1109, 1490, 1886, 1976, 2011
HpyF10VI GCNNNNNNNGC 6 cut(s) 242, 314, 521, 782, 1798, 1982
HpyF3I CTNAG 8 cut(s) 181, 237, 504, 772, 1178, 1473, 1730, 1981
HpySE526I ACGT 2 cut(s) 1643, 2339
HspAI GCGC 1 cut(s) 2327
KpnI GGTACC 1 cut(s) 8
KspAI GTTAAC 1 cut(s) 1995
Kzo9I GATC 7 cut(s) 114, 844, 957, 1102, 1552, 1953, 2229
LmnI GCTCC 4 cut(s) 188, 533, 691, 1310
Lsp1109I GCAGC 6 cut(s) 317, 508, 1325, 1474, 2023, 2182
MaeI CTAG 3 cut(s) 441, 1157, 2172
MaeII ACGT 2 cut(s) 1643, 2339
MaeIII GTNAC 5 cut(s) 817, 1124, 1967, 2051, 2140
MalI GATC 7 cut(s) 116, 846, 959, 1104, 1554, 1955, 2231
MboI GATC 7 cut(s) 114, 844, 957, 1102, 1552, 1953, 2229
MboII GAAGA 9 cut(s) 82, 414, 581, 938, 1133, 1345, 1547, 1565, 1700
MfeI CAATTG 1 cut(s) 1909
MflI RGATCY 1 cut(s) 844
MhlI GDGCHC 2 cut(s) 634, 1803
MlsI TGGCCA 1 cut(s) 1357
MluNI TGGCCA 1 cut(s) 1357
MlyI GAGTC 2 cut(s) 491, 863
MmeI TCCRAC 7 cut(s) 511, 528, 1030, 1191, 1302, 1406, 2245
Mox20I TGGCCA 1 cut(s) 1357
MroXI GAANNNNTTC 3 cut(s) 74, 555, 950
MscI TGGCCA 1 cut(s) 1357
MslI CAYNNNNRTG 2 cut(s) 212, 669
Msp20I TGGCCA 1 cut(s) 1357
MspI CCGG 3 cut(s) 1192, 1755, 1804
MspR9I CCNGG 5 cut(s) 45, 111, 277, 1359, 1805
MunI CAATTG 1 cut(s) 1909
Mva1269I GAATGC 2 cut(s) 25, 2018
MvaI CCWGG 4 cut(s) 45, 111, 277, 1359
MvnI CGCG 1 cut(s) 1538
MwoI GCNNNNNNNGC 6 cut(s) 242, 314, 521, 782, 1798, 1982
NciI CCSGG 1 cut(s) 1805
NcoI CCATGG 4 cut(s) 57, 213, 864, 2259
NdeI CATATG 4 cut(s) 13, 738, 1009, 1519
NdeII GATC 7 cut(s) 114, 844, 957, 1102, 1552, 1953, 2229
NlaIV GGNNCC 5 cut(s) 6, 757, 1070, 1277, 2133
NmuCI GTSAC 1 cut(s) 1124
NspI RCATGY 1 cut(s) 245
NspV TTCGAA 1 cut(s) 1443
PaeI GCATGC 1 cut(s) 245
PaeR7I CTCGAG 3 cut(s) 633, 714, 1340
PasI CCCWGGG 1 cut(s) 110
PctI GAATGC 2 cut(s) 25, 2018
PdmI GAANNNNTTC 3 cut(s) 74, 555, 950
PfeI GAWTC 5 cut(s) 281, 409, 951, 1464, 1658
PkrI GCNGC 6 cut(s) 307, 523, 1315, 1489, 2013, 2197
PleI GAGTC 2 cut(s) 491, 863
PpsI GAGTC 2 cut(s) 491, 863
PpuMI RGGWCCY 2 cut(s) 1068, 1826
PshAI GACNNNNGTC 1 cut(s) 1131
PsiI TTATAA 1 cut(s) 669
Psp5II RGGWCCY 2 cut(s) 1068, 1826
Psp6I CCWGG 4 cut(s) 43, 109, 275, 1357
PspEI GGTNACC 1 cut(s) 2140
PspFI CCCAGC 1 cut(s) 397
PspGI CCWGG 4 cut(s) 43, 109, 275, 1357
PspN4I GGNNCC 5 cut(s) 6, 757, 1070, 1277, 2133
PspPI GGNCC 7 cut(s) 359, 733, 756, 1068, 1826, 2267, 2300
PspPPI RGGWCCY 2 cut(s) 1068, 1826
PspXI VCTCGAGB 2 cut(s) 633, 714
PsrI GAACNNNNNNTAC 2 cut(s) 147, 179
PstI CTGCAG 1 cut(s) 1492
PsuI RGATCY 1 cut(s) 844
RsaI GTAC 8 cut(s) 6, 150, 581, 639, 1364, 1950, 2001, 2255
RsaNI GTAC 8 cut(s) 5, 149, 580, 638, 1363, 1949, 2000, 2254
RseI CAYNNNNRTG 2 cut(s) 212, 669
SatI GCNGC 6 cut(s) 306, 522, 1314, 1488, 2012, 2196
Sau3AI GATC 7 cut(s) 114, 844, 957, 1102, 1552, 1953, 2229
Sau96I GGNCC 7 cut(s) 359, 733, 756, 1068, 1826, 2267, 2300
SchI GAGTC 2 cut(s) 491, 863
ScrFI CCNGG 5 cut(s) 45, 111, 277, 1359, 1805
SduI GDGCHC 2 cut(s) 634, 1803
SfcI CTRYAG 2 cut(s) 1368, 1488
Sfr274I CTCGAG 3 cut(s) 633, 714, 1340
SfuI TTCGAA 1 cut(s) 1443
SinI GGWCC 7 cut(s) 359, 733, 756, 1068, 1826, 2267, 2300
SlaI CTCGAG 3 cut(s) 633, 714, 1340
SmiI ATTTAAAT 1 cut(s) 2109
SmiMI CAYNNNNRTG 2 cut(s) 212, 669
SmlI CTYRAG 5 cut(s) 633, 714, 1340, 1796, 2222
SmoI CTYRAG 5 cut(s) 633, 714, 1340, 1796, 2222
SpeI ACTAGT 1 cut(s) 440
SphI GCATGC 1 cut(s) 245
SsiI CCGC 2 cut(s) 1248, 1538
SspI AATATT 1 cut(s) 726
SspMI CTAG 3 cut(s) 441, 1157, 2172
StyD4I CCNGG 5 cut(s) 43, 109, 275, 1357, 1803
StyI CCWWGG 5 cut(s) 57, 213, 751, 864, 2259
SwaI ATTTAAAT 1 cut(s) 2109
TaaI ACNGT 4 cut(s) 1018, 1209, 1999, 2246
TaiI ACGT 2 cut(s) 1646, 2342
TaqI TCGA 6 cut(s) 117, 634, 715, 833, 1341, 1443
TatI WGTACW 2 cut(s) 1999, 2253
TfiI GAWTC 5 cut(s) 281, 409, 951, 1464, 1658
TscAI CASTG 5 cut(s) 189, 1044, 1186, 1978, 2029
TseFI GTSAC 1 cut(s) 1124
TseI GCWGC 6 cut(s) 305, 521, 1313, 1487, 2011, 2195
Tsp45I GTSAC 1 cut(s) 1124
TspDTI ATGAA 9 cut(s) 76, 82, 94, 582, 1443, 1506, 1559, 1756, 2223
TspGWI ACGGA 3 cut(s) 597, 836, 1218
TspRI CASTG 5 cut(s) 189, 1044, 1186, 1978, 2029
VpaK11BI GGWCC 7 cut(s) 359, 733, 756, 1068, 1826, 2267, 2300
XapI RAATTY 9 cut(s) 175, 681, 761, 1166, 1186, 1430, 1439, 1566, 2110
XbaI TCTAGA 1 cut(s) 2171
XceI RCATGY 1 cut(s) 245
XcmI CCANNNNNNNNNTGG 1 cut(s) 2257
XhoI CTCGAG 3 cut(s) 633, 714, 1340
XmiI GTMKAC 1 cut(s) 609
XmnI GAANNNNTTC 3 cut(s) 74, 555, 950
XspI CTAG 3 cut(s) 441, 1157, 2172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.