Rorug04G0046400

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001930
Physical Location & Seq
Reverse (-)
7212826 .. 7217194
4369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0046400.1

Sequence Viewer

Length: 756 bp
ATGAAGATAATGGTGGCCATAAAGCGCGTGGTCGACTACGCCGTCAAAATCCGAGTCAAGTCCGACAAGAGCGGCGTGGAGACCCAGAACGTGAAGATGTCGATGAACCCATTTTGCGAGATTGCTTTGGAAGAGGCCCTTCGGATCAAAGAATCGGGCTTGGCCTCCGAGGTCGTGGCGGTCACCATGGGCATGAAACAGAGCGTCGATACGTTAAGGACGGGTCTGGCTATGGGCGCTGATAGGGCTATACACGTGGAGACTAGTGGGCAGTTATACCCTTTATCGGTTGCTAAGCTTTTGAGAGCTTTAGTGGAGCTTGAGAAGCCTGGTCTTCTCATTCTGGGCAAACAGGCTATTGATGATGATTGCAATCAAACTGGGCAAATGGTGGCAGGGCTGTTGAACTGGCCTCAAGGCACTTTTGCTTCAAAGGTTGTGCTGGATAAGGAGAAGCAGGTAGTGACAGTGGACAGAGAGGTAGATGGTGGTCTTGAGACTCTCTGTCTAGATTTACCAGCAGTGATTACCACTGATTTGAGGCTAAATCAACCAAGGTATGCAACACTCCCCAACATAATGAAAGCAAAATCAAAGGTCATAAAGAAGTTCACTCCACAGGATCTGAATGTGGAAATCAAATCCGACATAGAAGAGGTTCAAGTCACAGAACCTCCCAAGAGAAAAGCAGGGGTTATCGTTTCTTCCGTGGATGAGCTGATTGACAAGCTGAAAAACGAAGCCCGTGTCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

251

Amino Acids

27.55

Weight (kDa)

7.62

Isoelectric Point (pI)

31.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ETF PF01012 26 - 208 1.6e-39 Electron transfer flavoprotein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 41
Acc36I ACCTGC 1 cut(s) 448
AccBSI CCGCTC 1 cut(s) 72
AccI GTMKAC 1 cut(s) 33
AccII CGCG 1 cut(s) 27
AciI CCGC 2 cut(s) 72, 179
AclWI GGATC 2 cut(s) 152, 630
AcoI YGGCCR 1 cut(s) 15
AcvI CACGTG 1 cut(s) 256
AfiI CCNNNNNNNGG 1 cut(s) 286
AflIII ACRYGT 1 cut(s) 253
AgsI TTSAA 3 cut(s) 406, 432, 662
AhdI GACNNNNNGTC 1 cut(s) 504
AhlI ACTAGT 1 cut(s) 263
AjnI CCWGG 1 cut(s) 328
AluBI AGCT 5 cut(s) 298, 308, 319, 718, 730
AluI AGCT 5 cut(s) 298, 308, 319, 718, 730
Alw26I GTCTC 3 cut(s) 74, 254, 491
AlwI GGATC 2 cut(s) 152, 630
AlwNI CAGNNNCTG 1 cut(s) 625
AoxI GGCC 4 cut(s) 15, 135, 162, 410
ArsI GACNNNNNNTTYG 3 cut(s) 39, 71, 732
Asp700I GAANNNNTTC 1 cut(s) 657
AspLEI GCGC 2 cut(s) 27, 239
AspS9I GGNCC 1 cut(s) 136
AsuHPI GGTGA 1 cut(s) 175
BalI TGGCCA 1 cut(s) 17
BbrPI CACGTG 1 cut(s) 256
BbsI GAAGAC 1 cut(s) 326
BccI CCATC 1 cut(s) 479
BceAI ACGGC 1 cut(s) 26
BciT130I CCWGG 1 cut(s) 330
BcoDI GTCTC 3 cut(s) 74, 254, 491
BcuI ACTAGT 1 cut(s) 263
BfaI CTAG 2 cut(s) 264, 509
BfoI RGCGCY 1 cut(s) 240
BfuAI ACCTGC 1 cut(s) 448
BisI GCNGC 1 cut(s) 73
BlpI GCTNAGC 1 cut(s) 294
BlsI GCNGC 1 cut(s) 74
Bme1390I CCNGG 1 cut(s) 330
BmeRI GACNNNNNGTC 1 cut(s) 504
BmgT120I GGNCC 1 cut(s) 136
BmrFI CCNGG 1 cut(s) 330
BmrI ACTGGG 1 cut(s) 390
BmuI ACTGGG 1 cut(s) 390
BpiI GAAGAC 1 cut(s) 326
Bpu1102I GCTNAGC 1 cut(s) 294
BpuEI CTTGAG 3 cut(s) 341, 399, 515
BsaAI YACGTR 1 cut(s) 256
BsaBI GATNNNNATC 1 cut(s) 372
BsaI GGTCTC 1 cut(s) 74
BsaJI CCNNGG 4 cut(s) 168, 186, 554, 708
BsaXI ACNNNNNCTCC 2 cut(s) 658, 688
Bsc4I CCNNNNNNNGG 1 cut(s) 286
Bse1I ACTGG 2 cut(s) 385, 413
Bse8I GATNNNNATC 1 cut(s) 372
BseBI CCWGG 1 cut(s) 330
BseDI CCNNGG 4 cut(s) 168, 186, 554, 708
BseGI GGATG 1 cut(s) 718
BseJI GATNNNNATC 1 cut(s) 372
BseLI CCNNNNNNNGG 1 cut(s) 286
BseNI ACTGG 2 cut(s) 385, 413
Bsh1236I CGCG 1 cut(s) 27
BshFI GGCC 4 cut(s) 17, 137, 164, 412
BslI CCNNNNNNNGG 1 cut(s) 286
BsmAI GTCTC 3 cut(s) 74, 254, 491
BsnI GGCC 4 cut(s) 17, 137, 164, 412
Bso31I GGTCTC 1 cut(s) 74
Bsp143I GATC 2 cut(s) 144, 622
Bsp1720I GCTNAGC 1 cut(s) 294
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 2 cut(s) 72, 179
BspANI GGCC 4 cut(s) 17, 137, 164, 412
BspFNI CGCG 1 cut(s) 27
BspMI ACCTGC 1 cut(s) 448
BspPI GGATC 2 cut(s) 152, 630
BspTNI GGTCTC 1 cut(s) 74
BsrBI CCGCTC 1 cut(s) 72
BsrI ACTGG 2 cut(s) 385, 413
BssECI CCNNGG 4 cut(s) 168, 186, 554, 708
BssMI GATC 2 cut(s) 144, 622
BssT1I CCWWGG 2 cut(s) 186, 554
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 1 cut(s) 469
Bst6I CTCTTC 2 cut(s) 126, 648
BstBAI YACGTR 1 cut(s) 256
BstDEI CTNAG 1 cut(s) 294
BstDSI CCRYGG 2 cut(s) 186, 708
BstEII GGTNACC 1 cut(s) 181
BstF5I GGATG 1 cut(s) 718
BstFNI CGCG 1 cut(s) 27
BstH2I RGCGCY 1 cut(s) 240
BstHHI GCGC 2 cut(s) 27, 239
BstKTI GATC 2 cut(s) 147, 625
BstMAI GTCTC 3 cut(s) 74, 254, 491
BstMBI GATC 2 cut(s) 144, 622
BstMWI GCNNNNNNNGC 3 cut(s) 236, 245, 325
BstNI CCWGG 1 cut(s) 330
BstPI GGTNACC 1 cut(s) 181
BstSCI CCNGG 1 cut(s) 328
BstUI CGCG 1 cut(s) 27
BstV2I GAAGAC 1 cut(s) 326
BstX2I RGATCY 1 cut(s) 622
BstYI RGATCY 1 cut(s) 622
BsuRI GGCC 4 cut(s) 17, 137, 164, 412
BtgI CCRYGG 2 cut(s) 186, 708
BtsCI GGATG 1 cut(s) 718
BtsI GCAGTG 1 cut(s) 528
BtsIMutI CAGTG 3 cut(s) 474, 528, 531
BveI ACCTGC 1 cut(s) 448
CaiI CAGNNNCTG 1 cut(s) 625
CfoI GCGC 2 cut(s) 27, 239
Cfr13I GGNCC 1 cut(s) 136
CseI GACGC 1 cut(s) 193
CviAII CATG 2 cut(s) 187, 193
DdeI CTNAG 1 cut(s) 294
DpnI GATC 2 cut(s) 146, 624
DpnII GATC 2 cut(s) 144, 622
DrdI GACNNNNNNGTC 1 cut(s) 41
DriI GACNNNNNGTC 1 cut(s) 504
DseDI GACNNNNNNGTC 1 cut(s) 41
EaeI YGGCCR 1 cut(s) 15
Eam1104I CTCTTC 2 cut(s) 126, 648
Eam1105I GACNNNNNGTC 1 cut(s) 504
EarI CTCTTC 2 cut(s) 126, 648
Eco130I CCWWGG 2 cut(s) 186, 554
Eco31I GGTCTC 1 cut(s) 74
Eco72I CACGTG 1 cut(s) 256
Eco91I GGTNACC 1 cut(s) 181
EcoO109I RGGNCCY 1 cut(s) 136
EcoO65I GGTNACC 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 328
EcoT14I CCWWGG 2 cut(s) 186, 554
ErhI CCWWGG 2 cut(s) 186, 554
FaeI CATG 2 cut(s) 190, 196
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FatI CATG 2 cut(s) 186, 192
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 1 cut(s) 73
FokI GGATG 1 cut(s) 725
Fsp4HI GCNGC 1 cut(s) 73
FspBI CTAG 2 cut(s) 264, 509
GlaI GCGC 2 cut(s) 26, 238
GluI GCNGC 1 cut(s) 73
HaeII RGCGCY 1 cut(s) 240
HaeIII GGCC 4 cut(s) 17, 137, 164, 412
HgaI GACGC 1 cut(s) 193
HhaI GCGC 2 cut(s) 27, 239
Hin1II CATG 2 cut(s) 190, 196
Hin6I GCGC 2 cut(s) 25, 237
HinP1I GCGC 2 cut(s) 25, 237
HincII GTYRAC 1 cut(s) 34
HindII GTYRAC 1 cut(s) 34
HindIII AAGCTT 1 cut(s) 296
HinfI GANTC 3 cut(s) 54, 152, 499
HphI GGTGA 1 cut(s) 175
Hpy166II GTNNAC 3 cut(s) 34, 472, 612
Hpy188I TCNGA 6 cut(s) 53, 64, 144, 169, 627, 646
Hpy188III TCNNGA 2 cut(s) 494, 509
Hpy8I GTNNAC 3 cut(s) 34, 472, 612
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 1 cut(s) 149
HpyCH4III ACNGT 1 cut(s) 469
HpyCH4IV ACGT 3 cut(s) 90, 212, 255
HpyCH4V TGCA 2 cut(s) 372, 563
HpyF10VI GCNNNNNNNGC 3 cut(s) 236, 245, 325
HpyF3I CTNAG 1 cut(s) 294
HpySE526I ACGT 3 cut(s) 90, 212, 255
Hsp92II CATG 2 cut(s) 190, 196
HspAI GCGC 2 cut(s) 25, 237
Kzo9I GATC 2 cut(s) 144, 622
LmnI GCTCC 1 cut(s) 316
MaeI CTAG 2 cut(s) 264, 509
MaeII ACGT 3 cut(s) 90, 212, 255
MaeIII GTNAC 3 cut(s) 181, 463, 664
MalI GATC 2 cut(s) 146, 624
MbiI CCGCTC 1 cut(s) 72
MboI GATC 2 cut(s) 144, 622
MboII GAAGA 6 cut(s) 16, 106, 143, 326, 665, 696
MflI RGATCY 1 cut(s) 622
MlsI TGGCCA 1 cut(s) 17
MluNI TGGCCA 1 cut(s) 17
MlyI GAGTC 2 cut(s) 63, 493
MmeI TCCRAC 2 cut(s) 87, 669
MnlI CCTC 8 cut(s) 127, 163, 175, 423, 472, 534, 649, 684
Mox20I TGGCCA 1 cut(s) 17
MroXI GAANNNNTTC 1 cut(s) 657
MscI TGGCCA 1 cut(s) 17
MseI TTAA 1 cut(s) 215
MslI CAYNNNNRTG 1 cut(s) 191
Msp20I TGGCCA 1 cut(s) 17
MspR9I CCNGG 1 cut(s) 330
MvaI CCWGG 1 cut(s) 330
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 3 cut(s) 236, 245, 325
NcoI CCATGG 1 cut(s) 186
NdeII GATC 2 cut(s) 144, 622
NlaIII CATG 2 cut(s) 190, 196
NmuCI GTSAC 3 cut(s) 181, 463, 664
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 705
PdmI GAANNNNTTC 1 cut(s) 657
PfeI GAWTC 1 cut(s) 152
PkrI GCNGC 1 cut(s) 74
PleI GAGTC 2 cut(s) 62, 493
PmaCI CACGTG 1 cut(s) 256
PmlI CACGTG 1 cut(s) 256
PpsI GAGTC 2 cut(s) 62, 493
Ppu21I YACGTR 1 cut(s) 256
Psp6I CCWGG 1 cut(s) 328
PspCI CACGTG 1 cut(s) 256
PspEI GGTNACC 1 cut(s) 181
PspGI CCWGG 1 cut(s) 328
PspPI GGNCC 1 cut(s) 136
PstNI CAGNNNCTG 1 cut(s) 625
PsuI RGATCY 1 cut(s) 622
RseI CAYNNNNRTG 1 cut(s) 191
SalI GTCGAC 1 cut(s) 32
SaqAI TTAA 1 cut(s) 215
SatI GCNGC 1 cut(s) 73
Sau3AI GATC 2 cut(s) 144, 622
Sau96I GGNCC 1 cut(s) 136
SchI GAGTC 2 cut(s) 63, 493
ScrFI CCNGG 1 cut(s) 330
SmiMI CAYNNNNRTG 1 cut(s) 191
SmlI CTYRAG 3 cut(s) 320, 414, 494
SmoI CTYRAG 3 cut(s) 320, 414, 494
SpeI ACTAGT 1 cut(s) 263
SsiI CCGC 2 cut(s) 72, 179
SspMI CTAG 2 cut(s) 264, 509
StyD4I CCNGG 1 cut(s) 328
StyI CCWWGG 2 cut(s) 186, 554
TaaI ACNGT 1 cut(s) 469
TaiI ACGT 3 cut(s) 93, 215, 258
TaqI TCGA 3 cut(s) 33, 101, 207
TauI GCSGC 1 cut(s) 75
TfiI GAWTC 1 cut(s) 152
Tru1I TTAA 1 cut(s) 215
Tru9I TTAA 1 cut(s) 215
TscAI CASTG 3 cut(s) 474, 528, 538
TseFI GTSAC 3 cut(s) 181, 463, 664
Tsp45I GTSAC 3 cut(s) 181, 463, 664
TspDTI ATGAA 4 cut(s) 17, 119, 209, 596
TspGWI ACGGA 1 cut(s) 697
TspRI CASTG 3 cut(s) 474, 528, 538
XbaI TCTAGA 1 cut(s) 508
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XmiI GTMKAC 1 cut(s) 33
XmnI GAANNNNTTC 1 cut(s) 657
XspI CTAG 2 cut(s) 264, 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.