Rh4DG115700

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001930
Physical Location & Seq
Forward (+)
18265248 .. 18268725
3478 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG115700.1

Sequence Viewer

Length: 2487 bp
ATGGCAGAGAACTGCATGACCATGGCACTTGATGATTTTTATGCTAAGCATGCTCATTATCAAACAAGGCTTTTCTTTCTCACCAGGGATTCAAAGAATGATGTTGTTTCAGCAGCATTTGAAGCCCAGCACTTGATAAACATTGAAAATGTGAGTGCCATTATCGGACCTCAAAGTTCAGCAGAAGCAAAGTTTGTGATAGAGCTGGGAAGAAGGAATCATGTTCCCATTCTTTCGTTTTCTGCCACTAGTCCATCTCTTTCTCCATCCCATAGTTCATTTTTTGTTCGGACGGCTTTTAGTGACTCTGCTCAAGTAGAAGCCATAGCTGCCATTGTCGGAGCTTATAGATGGTTGGAAGTTGTTCTCATCTATGAAGACACCGAGTATGGAAATAGCTTAATTCCATATTTGGTAGACGCTTTCCAGCAAGTTGGCGCTCGAGTGCCTTATAGAAATGTCATTCCTCCTCATTATAATGGCACAAAAATTTTAAAGGAGCTTAACCACTTGAAATCAACTCGAGCAAGAATATTTTTGGTCCATATGACTGCTTCCCTTGGGTCCAAATTCTTTTTCTTAGCAAGTAAGGCAGGGATGATGACTGAAGGGTATGGATGGATTGTTACGGAAGGGTTATCGACTTTATTAGATCCTGTGGGTTCAAGAACCATGGACTCAATGCAAGCTGTAGTGGGGGTAAGGCCATATATACCAATGTCAAAAGAACTTAAAGACTTCGTGTTGCGAATGAAAAGATTCAGGTTCAGAAAATCAAGCAAGATTACTGGTATAAATCTCTTTGGGTTGTGGGCATATGATACAGTTTGGGCATTAGCAATGGCAGTGGAGAAGGTTGGAATAGCAAATTATAGTACCATGATACAAAATGTTAGCACAAGCAGAGTTGATCTTGCAAGTTTGGGAACTGGTGACAGGGGTCAAAATCTTCTTGAAGCTATCCTAGATTTAAAATTTGAAAACCTCAGTTGGAATTTCCGGTTGGCTAAGGGACAGTTGGAAGCTTCAACCTTTGAGGTATTTAATGTGATTGGCGGAAAAGAAAGAATAATTGGATATTGGAACCAAATGAAAGGACTTTCCCGGAAGCTGAATGGAGCAGCAAAGTTGGATGTAAGGAAGACACTCGAGAAACCAATCTGGCCAGGGTACACTACAGACATCCCTCCAACAAAGAAATTGAGGATTGGAGTTCCCTTGAAAAAAGGTTTTTATGAATTCCTAAAATTCGAAAACAACAATATATCAGGATTCTCTGCTGAGATATTCTTTGCTGCAGAAAAGAAACTGCCATTTTCCCTTTCATATGAGTTTTCTGCCTTCGCGGGGACTTATGATGATCTTCTTTATCAAATTCATCTTCAGAAATATGATGCTGTGGTGGGAGACACAACAATTGTAGCTAATCGCTCATTATATGTAGATTTTACGTTGCCTTATTCTGAATCAGGAGTTTCCATGATAGTTTTGACAGAAGATAGTGAGAGGAATAGTGTTTGGATTTTCTTGAAGCCACTAAGCTTGGGTCTTTGGTTGACAACCGGCGTAGCCTTCATATTTACAGGTTTTGTAATATGGCTTCTTGAGCACCGGGAAAACACCGAGTTCAGAGGTCCTCCACAGAAACAACTTGGCATGATATTCTGGTTCTCCTTTTCAACCCTCGTCTTTGCACACAGAGAGAAAGTGGTGAACAATTGGTCAAGGTTTGTGCTGATTATATGGGTTTTTGTGGTACTGATCCTAACACAGAGTTACACTGCAAGCTTAGCCTCGATGTTAACAGTACAGAAATTGCAGCCTGCATTCACTGATATAAAAGAGATACAAGGAAATGGTTACAAAGTAGGATATCAAAATAGTTCCTTTATTAAAGGGTTTCTCATGGAACATTTAAATTTTGATGAGACCCAGTTGGTGCCATTGGTAACCATGCAGGATTATCATAACGCACTCTCTAGAGGAAATAAGAATAGAGTTGCTGCCATTTTAGATGAAGTCCCTTACCTCAAGTTGATCCTTAAAAAGAACTGTTCCAAGTACACCATGGTTGGACCAACCTACAAAACTGATGGATTTGGCTTTGCTTTCCAATCAGGGTCCTCGCTGGTATCCTACATGTCAAGCGCAATCTTGAACGTGACTCAAGATAAAAATCAGATGGATAGACTTCAGTCGACATACTTTGATTACTTTGATAACCAACCTGCCTGTGAGGATCAAAGTGCCAAAATTTCTTCAGAGGGTCCAAGTCTAGGTGTCTACAGCTTTGGAGGCCTCTTCATCATCGCAGGTGTGGCCTCCACGGTTGCTCTTTTGATGTACATGTATCACTTCCTTTGCTCTCATTGGCCAGCTTTGCGAATAATTAGTTTTTCTGAGAGCAGATTTTGGGAGAAATTGGTTGAAGTGGCAAAGCATTTTGACAAGAAAGCACTATGCCAAAAAGGCCTTCAGACGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

828

Amino Acids

93.47

Weight (kDa)

8.94

Isoelectric Point (pI)

33.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANF_receptor PF01094 3 - 352 9.6e-75 Receptor family ligand binding region
Peripla_BP_6 PF13458 3 - 304 9.1e-21 Periplasmic binding protein
SBP_bac_3 PF00497 412 - 736 5.7e-10 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 515 - 774 2.9e-36 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 477
AarI CACCTGC 1 cut(s) 2303
Acc36I ACCTGC 2 cut(s) 2236, 2303
AccB1I GGYRCC 1 cut(s) 1939
AccI GTMKAC 3 cut(s) 417, 2198, 2283
AccII CGCG 1 cut(s) 1346
AciI CCGC 2 cut(s) 1056, 1346
AclWI GGATC 4 cut(s) 647, 1756, 2032, 2247
AcoI YGGCCR 2 cut(s) 1163, 2372
AcsI RAATTY 9 cut(s) 489, 569, 974, 994, 1238, 1247, 1374, 1918, 2253
AcuI CTGAAG 5 cut(s) 627, 1367, 2177, 2244, 2459
AfaI GTAC 6 cut(s) 877, 1172, 1758, 1809, 2063, 2345
AfiI CCNNNNNNNGG 2 cut(s) 1347, 2276
AflIII ACRYGT 2 cut(s) 2139, 2346
AhlI ACTAGT 1 cut(s) 248
AjnI CCWGG 2 cut(s) 83, 1165
AjuI GAANNNNNNNTTGG 7 cut(s) 560, 592, 986, 1018, 1056, 1088, 2457
Alw21I GWGCWC 1 cut(s) 1611
Alw26I GTCTC 2 cut(s) 1401, 1922
AlwI GGATC 4 cut(s) 647, 1756, 2032, 2247
Ama87I CYCGRG 3 cut(s) 441, 522, 1148
AoxI GGCC 6 cut(s) 704, 1163, 2296, 2319, 2372, 2470
ApeKI GCWGC 6 cut(s) 113, 329, 1121, 1295, 1819, 2003
ApoI RAATTY 9 cut(s) 489, 569, 974, 994, 1238, 1247, 1374, 1918, 2253
Asp700I GAANNNNTTC 2 cut(s) 363, 758
AspLEI GCGC 2 cut(s) 440, 2150
AspS9I GGNCC 7 cut(s) 167, 541, 564, 1634, 2075, 2121, 2267
AsuC2I CCSGG 2 cut(s) 1105, 1613
AsuHPI GGTGA 3 cut(s) 73, 944, 1723
AsuII TTCGAA 1 cut(s) 1251
AvaI CYCGRG 3 cut(s) 441, 522, 1148
AvaII GGWCC 7 cut(s) 167, 541, 564, 1634, 2075, 2121, 2267
BalI TGGCCA 2 cut(s) 1165, 2374
BanI GGYRCC 1 cut(s) 1939
BarI GAAGNNNNNNTAC 2 cut(s) 1584, 1616
BbsI GAAGAC 2 cut(s) 384, 1148
Bbv12I GWGCWC 1 cut(s) 1611
BbvI GCAGC 6 cut(s) 125, 316, 1133, 1282, 1831, 1990
BccI CCATC 6 cut(s) 262, 274, 345, 612, 2087, 2176
BceAI ACGGC 1 cut(s) 309
BciT130I CCWGG 2 cut(s) 85, 1167
BciVI GTATCC 1 cut(s) 2143
BcnI CCSGG 2 cut(s) 1105, 1613
BcoDI GTCTC 2 cut(s) 1401, 1922
BcuI ACTAGT 1 cut(s) 248
BfaI CTAG 4 cut(s) 249, 965, 1980, 2276
BfmI CTRYAG 4 cut(s) 690, 1176, 1296, 2284
BfoI RGCGCY 1 cut(s) 441
BfuAI ACCTGC 2 cut(s) 2236, 2303
BfuI GTATCC 1 cut(s) 2143
BglI GCCNNNNNGGC 1 cut(s) 2469
BisI GCNGC 6 cut(s) 114, 330, 1122, 1296, 1820, 2004
BlpI GCTNAGC 2 cut(s) 45, 1789
BlsI GCNGC 6 cut(s) 115, 331, 1123, 1297, 1821, 2005
Bme1390I CCNGG 4 cut(s) 85, 1105, 1167, 1613
Bme18I GGWCC 7 cut(s) 167, 541, 564, 1634, 2075, 2121, 2267
BmeT110I CYCGRG 3 cut(s) 441, 522, 1148
BmgT120I GGNCC 7 cut(s) 167, 541, 564, 1634, 2075, 2121, 2267
BmiI GGNNCC 5 cut(s) 565, 1085, 1941, 2122, 2268
BmrFI CCNGG 4 cut(s) 85, 1105, 1167, 1613
BmrI ACTGGG 1 cut(s) 1927
BmsI GCATC 1 cut(s) 1384
BmuI ACTGGG 1 cut(s) 1927
BoxI GACNNNNGTC 2 cut(s) 939, 2194
BpiI GAAGAC 2 cut(s) 384, 1148
Bpu10I CCTNAGC 1 cut(s) 1008
Bpu1102I GCTNAGC 2 cut(s) 45, 1789
Bpu14I TTCGAA 1 cut(s) 1251
BpuEI CTTGAG 4 cut(s) 297, 1625, 2015, 2151
BpuMI CCSGG 2 cut(s) 1105, 1613
BsaBI GATNNNNATC 1 cut(s) 2175
BsaI GGTCTC 1 cut(s) 1922
BsaJI CCNNGG 7 cut(s) 21, 84, 559, 672, 1166, 2067, 2325
BsaWI WCCGGW 1 cut(s) 999
Bsc4I CCNNNNNNNGG 2 cut(s) 1347, 2276
Bse118I RCCGGY 1 cut(s) 1562
Bse1I ACTGG 3 cut(s) 793, 934, 1933
Bse3DI GCAATG 1 cut(s) 846
Bse8I GATNNNNATC 1 cut(s) 2175
BseBI CCWGG 2 cut(s) 85, 1167
BseDI CCNNGG 7 cut(s) 21, 84, 559, 672, 1166, 2067, 2325
BseGI GGATG 5 cut(s) 266, 603, 623, 1138, 1182
BseJI GATNNNNATC 1 cut(s) 2175
BseLI CCNNNNNNNGG 2 cut(s) 1347, 2276
BseMI GCAATG 1 cut(s) 846
BseMII CTCAG 3 cut(s) 1000, 1272, 2391
BseNI ACTGG 3 cut(s) 793, 934, 1933
BseRI GAGGAG 1 cut(s) 459
BseXI GCAGC 6 cut(s) 125, 316, 1133, 1282, 1831, 1990
BseYI CCCAGC 2 cut(s) 126, 205
Bsh1236I CGCG 1 cut(s) 1346
BshFI GGCC 6 cut(s) 706, 1165, 2298, 2321, 2374, 2472
BshNI GGYRCC 1 cut(s) 1939
BsiHKAI GWGCWC 1 cut(s) 1611
BsiHKCI CYCGRG 3 cut(s) 441, 522, 1148
BsiSI CCGG 4 cut(s) 1000, 1105, 1563, 1612
BslFI GGGAC 3 cut(s) 1026, 1363, 2006
BslI CCNNNNNNNGG 2 cut(s) 1347, 2276
BsmAI GTCTC 2 cut(s) 1401, 1922
BsmFI GGGAC 3 cut(s) 1026, 1363, 2006
BsmI GAATGC 1 cut(s) 1826
BsnI GGCC 6 cut(s) 706, 1165, 2298, 2321, 2374, 2472
Bso31I GGTCTC 1 cut(s) 1922
BsoBI CYCGRG 3 cut(s) 441, 522, 1148
Bsp119I TTCGAA 1 cut(s) 1251
Bsp1286I GDGCHC 1 cut(s) 1611
Bsp1407I TGTACA 1 cut(s) 2343
Bsp143I GATC 6 cut(s) 652, 910, 1360, 1761, 2037, 2239
Bsp1720I GCTNAGC 2 cut(s) 45, 1789
Bsp19I CCATGG 3 cut(s) 21, 672, 2067
BspACI CCGC 2 cut(s) 1056, 1346
BspANI GGCC 6 cut(s) 706, 1165, 2298, 2321, 2374, 2472
BspCNI CTCAG 3 cut(s) 999, 1273, 2392
BspFNI CGCG 1 cut(s) 1346
BspLI GGNNCC 5 cut(s) 565, 1085, 1941, 2122, 2268
BspMAI CTGCAG 1 cut(s) 1300
BspMI ACCTGC 2 cut(s) 2236, 2303
BspPI GGATC 4 cut(s) 647, 1756, 2032, 2247
BspT104I TTCGAA 1 cut(s) 1251
BspT107I GGYRCC 1 cut(s) 1939
BspTNI GGTCTC 1 cut(s) 1922
BsrDI GCAATG 1 cut(s) 846
BsrFI RCCGGY 1 cut(s) 1562
BsrGI TGTACA 1 cut(s) 2343
BsrI ACTGG 3 cut(s) 793, 934, 1933
BssAI RCCGGY 1 cut(s) 1562
BssECI CCNNGG 7 cut(s) 21, 84, 559, 672, 1166, 2067, 2325
BssMI GATC 6 cut(s) 652, 910, 1360, 1761, 2037, 2239
BssT1I CCWWGG 4 cut(s) 21, 559, 672, 2067
Bst2UI CCWGG 2 cut(s) 85, 1167
Bst4CI ACNGT 5 cut(s) 826, 1017, 1807, 2054, 2329
Bst6I CTCTTC 1 cut(s) 2306
BstAUI TGTACA 1 cut(s) 2343
BstBI TTCGAA 1 cut(s) 1251
BstC8I GCNNGC 5 cut(s) 51, 687, 1786, 1824, 2376
BstDEI CTNAG 8 cut(s) 45, 580, 986, 1008, 1281, 1538, 1789, 2400
BstDSI CCRYGG 4 cut(s) 21, 672, 2067, 2325
BstEII GGTNACC 1 cut(s) 1948
BstF5I GGATG 5 cut(s) 266, 603, 623, 1138, 1182
BstFNI CGCG 1 cut(s) 1346
BstH2I RGCGCY 1 cut(s) 441
BstHHI GCGC 2 cut(s) 440, 2150
BstKTI GATC 6 cut(s) 655, 913, 1363, 1764, 2040, 2242
BstMAI GTCTC 2 cut(s) 1401, 1922
BstMBI GATC 6 cut(s) 652, 910, 1360, 1761, 2037, 2239
BstNI CCWGG 2 cut(s) 85, 1167
BstNSI RCATGY 3 cut(s) 53, 2143, 2350
BstPAI GACNNNNGTC 2 cut(s) 939, 2194
BstPI GGTNACC 1 cut(s) 1948
BstSCI CCNGG 4 cut(s) 83, 1103, 1165, 1611
BstSFI CTRYAG 4 cut(s) 690, 1176, 1296, 2284
BstUI CGCG 1 cut(s) 1346
BstV1I GCAGC 6 cut(s) 125, 316, 1133, 1282, 1831, 1990
BstV2I GAAGAC 2 cut(s) 384, 1148
BstX2I RGATCY 1 cut(s) 652
BstXI CCANNNNNNTGG 1 cut(s) 434
BstYI RGATCY 1 cut(s) 652
BsuI GTATCC 1 cut(s) 2143
BsuRI GGCC 6 cut(s) 706, 1165, 2298, 2321, 2374, 2472
BtgI CCRYGG 4 cut(s) 21, 672, 2067, 2325
BtgZI GCGATG 1 cut(s) 2293
BtsCI GGATG 5 cut(s) 266, 603, 623, 1138, 1182
BtsI GCAGTG 2 cut(s) 852, 1779
BtsIMutI CAGTG 3 cut(s) 852, 1779, 1830
BveI ACCTGC 2 cut(s) 2236, 2303
Cac8I GCNNGC 5 cut(s) 51, 687, 1786, 1824, 2376
CfoI GCGC 2 cut(s) 440, 2150
Cfr10I RCCGGY 1 cut(s) 1562
Cfr13I GGNCC 7 cut(s) 167, 541, 564, 1634, 2075, 2121, 2267
CseI GACGC 1 cut(s) 428
Csp6I GTAC 6 cut(s) 876, 1171, 1757, 1808, 2062, 2344
CspCI CAANNNNNGTGG 4 cut(s) 828, 863, 1524, 1559
CviQI GTAC 6 cut(s) 876, 1171, 1757, 1808, 2062, 2344
DdeI CTNAG 8 cut(s) 45, 580, 986, 1008, 1281, 1538, 1789, 2400
DpnI GATC 6 cut(s) 654, 912, 1362, 1763, 2039, 2241
DpnII GATC 6 cut(s) 652, 910, 1360, 1761, 2037, 2239
DraI TTTAAA 3 cut(s) 495, 972, 1917
EaeI YGGCCR 2 cut(s) 1163, 2372
Eam1104I CTCTTC 1 cut(s) 2306
EarI CTCTTC 1 cut(s) 2306
EciI GGCGGA 1 cut(s) 1071
Eco130I CCWWGG 4 cut(s) 21, 559, 672, 2067
Eco147I AGGCCT 2 cut(s) 2298, 2472
Eco31I GGTCTC 1 cut(s) 1922
Eco32I GATATC 1 cut(s) 1874
Eco47I GGWCC 7 cut(s) 167, 541, 564, 1634, 2075, 2121, 2267
Eco57I CTGAAG 5 cut(s) 627, 1367, 2177, 2244, 2459
Eco88I CYCGRG 3 cut(s) 441, 522, 1148
Eco91I GGTNACC 1 cut(s) 1948
EcoO109I RGGNCCY 2 cut(s) 1634, 2121
EcoO65I GGTNACC 1 cut(s) 1948
EcoRI GAATTC 1 cut(s) 1238
EcoRII CCWGG 2 cut(s) 83, 1165
EcoRV GATATC 1 cut(s) 1874
EcoT14I CCWWGG 4 cut(s) 21, 559, 672, 2067
ErhI CCWWGG 4 cut(s) 21, 559, 672, 2067
FaqI GGGAC 3 cut(s) 1026, 1363, 2006
FauI CCCGC 1 cut(s) 1339
FauNDI CATATG 3 cut(s) 546, 817, 1327
FblI GTMKAC 3 cut(s) 417, 2198, 2283
Fnu4HI GCNGC 6 cut(s) 114, 330, 1122, 1296, 1820, 2004
FokI GGATG 5 cut(s) 253, 610, 630, 1145, 1169
Fsp4HI GCNGC 6 cut(s) 114, 330, 1122, 1296, 1820, 2004
FspBI CTAG 4 cut(s) 249, 965, 1980, 2276
GlaI GCGC 2 cut(s) 439, 2149
GluI GCNGC 6 cut(s) 114, 330, 1122, 1296, 1820, 2004
GsaI CCCAGC 2 cut(s) 130, 209
HaeII RGCGCY 1 cut(s) 441
HaeIII GGCC 6 cut(s) 706, 1165, 2298, 2321, 2374, 2472
HapII CCGG 4 cut(s) 1000, 1105, 1563, 1612
HgaI GACGC 1 cut(s) 428
HhaI GCGC 2 cut(s) 440, 2150
Hin6I GCGC 2 cut(s) 438, 2148
HinP1I GCGC 2 cut(s) 438, 2148
HincII GTYRAC 3 cut(s) 1557, 1803, 2199
HindII GTYRAC 3 cut(s) 1557, 1803, 2199
HindIII AAGCTT 3 cut(s) 1023, 1540, 1786
HinfI GANTC 8 cut(s) 89, 217, 305, 677, 759, 1272, 1466, 2164
HpaI GTTAAC 1 cut(s) 1803
HpaII CCGG 4 cut(s) 1000, 1105, 1563, 1612
HphI GGTGA 3 cut(s) 73, 944, 1723
Hpy166II GTNNAC 8 cut(s) 418, 1173, 1557, 1714, 1803, 2064, 2199, 2284
Hpy8I GTNNAC 8 cut(s) 418, 1173, 1557, 1714, 1803, 2064, 2199, 2284
HpyAV CCTTC 7 cut(s) 207, 602, 626, 847, 1351, 1582, 2483
HpyCH4III ACNGT 5 cut(s) 826, 1017, 1807, 2054, 2329
HpyCH4IV ACGT 2 cut(s) 1451, 2160
HpyCH4V TGCA 9 cut(s) 15, 685, 917, 1298, 1694, 1784, 1819, 1826, 1957
HpyF3I CTNAG 8 cut(s) 45, 580, 986, 1008, 1281, 1538, 1789, 2400
HpySE526I ACGT 2 cut(s) 1451, 2160
HspAI GCGC 2 cut(s) 438, 2148
KspAI GTTAAC 1 cut(s) 1803
Kzo9I GATC 6 cut(s) 652, 910, 1360, 1761, 2037, 2239
LmnI GCTCC 3 cut(s) 341, 499, 1118
Lsp1109I GCAGC 6 cut(s) 125, 316, 1133, 1282, 1831, 1990
LweI GCATC 1 cut(s) 1384
MaeI CTAG 4 cut(s) 249, 965, 1980, 2276
MaeII ACGT 2 cut(s) 1451, 2160
MaeIII GTNAC 7 cut(s) 302, 625, 932, 1775, 1859, 1948, 2161
MalI GATC 6 cut(s) 654, 912, 1362, 1763, 2039, 2241
MboI GATC 6 cut(s) 652, 910, 1360, 1761, 2037, 2239
MboII GAAGA 9 cut(s) 222, 389, 941, 1153, 1355, 1373, 1508, 2250, 2293
MfeI CAATTG 2 cut(s) 1416, 1717
MflI RGATCY 1 cut(s) 652
MhlI GDGCHC 1 cut(s) 1611
MlsI TGGCCA 2 cut(s) 1165, 2374
MluNI TGGCCA 2 cut(s) 1165, 2374
MlyI GAGTC 3 cut(s) 299, 671, 2158
MmeI TCCRAC 8 cut(s) 319, 336, 838, 971, 999, 1110, 1214, 2053
Mox20I TGGCCA 2 cut(s) 1165, 2374
MroXI GAANNNNTTC 2 cut(s) 363, 758
MscI TGGCCA 2 cut(s) 1165, 2374
MslI CAYNNNNRTG 2 cut(s) 20, 477
Msp20I TGGCCA 2 cut(s) 1165, 2374
MspI CCGG 4 cut(s) 1000, 1105, 1563, 1612
MspR9I CCNGG 4 cut(s) 85, 1105, 1167, 1613
MunI CAATTG 2 cut(s) 1416, 1717
Mva1269I GAATGC 1 cut(s) 1826
MvaI CCWGG 2 cut(s) 85, 1167
MvnI CGCG 1 cut(s) 1346
NciI CCSGG 2 cut(s) 1105, 1613
NcoI CCATGG 3 cut(s) 21, 672, 2067
NdeI CATATG 3 cut(s) 546, 817, 1327
NdeII GATC 6 cut(s) 652, 910, 1360, 1761, 2037, 2239
NlaIV GGNNCC 5 cut(s) 565, 1085, 1941, 2122, 2268
NmuCI GTSAC 3 cut(s) 302, 932, 2161
NspI RCATGY 3 cut(s) 53, 2143, 2350
NspV TTCGAA 1 cut(s) 1251
PaeI GCATGC 1 cut(s) 53
PaeR7I CTCGAG 3 cut(s) 441, 522, 1148
PaqCI CACCTGC 1 cut(s) 2303
PceI AGGCCT 2 cut(s) 2298, 2472
PciI ACATGT 2 cut(s) 2139, 2346
PctI GAATGC 1 cut(s) 1826
PdmI GAANNNNTTC 2 cut(s) 363, 758
PfeI GAWTC 5 cut(s) 89, 217, 759, 1272, 1466
PfoI TCCNGGA 1 cut(s) 1103
PkrI GCNGC 6 cut(s) 115, 331, 1123, 1297, 1821, 2005
PleI GAGTC 3 cut(s) 299, 671, 2158
PpsI GAGTC 3 cut(s) 299, 671, 2158
PpuMI RGGWCCY 2 cut(s) 1634, 2121
PscI ACATGT 2 cut(s) 2139, 2346
PshAI GACNNNNGTC 2 cut(s) 939, 2194
PsiI TTATAA 1 cut(s) 477
Psp5II RGGWCCY 2 cut(s) 1634, 2121
Psp6I CCWGG 2 cut(s) 83, 1165
PspEI GGTNACC 1 cut(s) 1948
PspFI CCCAGC 2 cut(s) 126, 205
PspGI CCWGG 2 cut(s) 83, 1165
PspN4I GGNNCC 5 cut(s) 565, 1085, 1941, 2122, 2268
PspPI GGNCC 7 cut(s) 167, 541, 564, 1634, 2075, 2121, 2267
PspPPI RGGWCCY 2 cut(s) 1634, 2121
PspXI VCTCGAGB 2 cut(s) 441, 522
PstI CTGCAG 1 cut(s) 1300
PsuI RGATCY 1 cut(s) 652
RsaI GTAC 6 cut(s) 877, 1172, 1758, 1809, 2063, 2345
RsaNI GTAC 6 cut(s) 876, 1171, 1757, 1808, 2062, 2344
RseI CAYNNNNRTG 2 cut(s) 20, 477
SalI GTCGAC 1 cut(s) 2197
SatI GCNGC 6 cut(s) 114, 330, 1122, 1296, 1820, 2004
Sau3AI GATC 6 cut(s) 652, 910, 1360, 1761, 2037, 2239
Sau96I GGNCC 7 cut(s) 167, 541, 564, 1634, 2075, 2121, 2267
SchI GAGTC 3 cut(s) 299, 671, 2158
ScrFI CCNGG 4 cut(s) 85, 1105, 1167, 1613
SduI GDGCHC 1 cut(s) 1611
SfaNI GCATC 1 cut(s) 1384
SfcI CTRYAG 4 cut(s) 690, 1176, 1296, 2284
Sfr274I CTCGAG 3 cut(s) 441, 522, 1148
SfuI TTCGAA 1 cut(s) 1251
SinI GGWCC 7 cut(s) 167, 541, 564, 1634, 2075, 2121, 2267
SlaI CTCGAG 3 cut(s) 441, 522, 1148
SmiI ATTTAAAT 1 cut(s) 1917
SmiMI CAYNNNNRTG 2 cut(s) 20, 477
SmlI CTYRAG 7 cut(s) 312, 441, 522, 1148, 1604, 2030, 2166
SmoI CTYRAG 7 cut(s) 312, 441, 522, 1148, 1604, 2030, 2166
SpeI ACTAGT 1 cut(s) 248
SphI GCATGC 1 cut(s) 53
SseBI AGGCCT 2 cut(s) 2298, 2472
SsiI CCGC 2 cut(s) 1056, 1346
SspI AATATT 1 cut(s) 534
SspMI CTAG 4 cut(s) 249, 965, 1980, 2276
StuI AGGCCT 2 cut(s) 2298, 2472
StyD4I CCNGG 4 cut(s) 83, 1103, 1165, 1611
StyI CCWWGG 4 cut(s) 21, 559, 672, 2067
SwaI ATTTAAAT 1 cut(s) 1917
TaaI ACNGT 5 cut(s) 826, 1017, 1807, 2054, 2329
TaiI ACGT 2 cut(s) 1454, 2163
TaqI TCGA 7 cut(s) 442, 523, 641, 1149, 1251, 1796, 2198
TatI WGTACW 3 cut(s) 1807, 2061, 2343
TfiI GAWTC 5 cut(s) 89, 217, 759, 1272, 1466
TscAI CASTG 3 cut(s) 852, 1786, 1837
TseFI GTSAC 3 cut(s) 302, 932, 2161
TseI GCWGC 6 cut(s) 113, 329, 1121, 1295, 1819, 2003
Tsp45I GTSAC 3 cut(s) 302, 932, 2161
TspGWI ACGGA 1 cut(s) 644
TspRI CASTG 3 cut(s) 852, 1786, 1837
VpaK11BI GGWCC 7 cut(s) 167, 541, 564, 1634, 2075, 2121, 2267
XapI RAATTY 9 cut(s) 489, 569, 974, 994, 1238, 1247, 1374, 1918, 2253
XbaI TCTAGA 1 cut(s) 1979
XceI RCATGY 3 cut(s) 53, 2143, 2350
XcmI CCANNNNNNNNNTGG 1 cut(s) 2065
XhoI CTCGAG 3 cut(s) 441, 522, 1148
XmiI GTMKAC 3 cut(s) 417, 2198, 2283
XmnI GAANNNNTTC 2 cut(s) 363, 758
XspI CTAG 4 cut(s) 249, 965, 1980, 2276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.