Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0001281

Physical Location & Seq

Genomic Coordinates Forward (+)

71861179 .. 71875670

Gene Structure 14492 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000021092

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2574 bp

ATGGTTTCGAGTTTTGGATCGATCCAGATGACTATCATGTTGGTATTAGTATACCACAACAGCAGATGGAAGATGAAGTTCCGGATCGGATGTGTGTTGTTTGTTGTGTTGTGCCTTTGTTCTGGAGCAGAGTCCCAGTGCAGTAGTAGCAAAAGCTGCGATGCTTTGGGTTCTTATTACATGTGGCAGGGCGCCGATCTCACCTTCGTCGGAACGGTAATGAACACCAGCGCCGACATCATCGCCAGCTACAACAAAGCGACGGTGCCGAACAAGGACAGTGTTCAAGCTGGTTTGAGGGTCAATGTTCCGTTCAGCTGTGGCTGCATAAATGAATCGTTTCCGGGCCACGTGTTCGAGTACGACGTCGTCTCCGGCGACACGTACGAATTGGTTGCGAGTAAGTACTATGCCAACCTGACCACGGCGGATGCCATGGCGTGGTTCAATGAGTACCCTGCGAATAACGTACCCAATACGAATACGAAGCTGAATGTGGTGGTCAACTGTTCGTGTGGGAATAGCGCGGTTTCCGAGGACTATGGGTTGTTTATCACGTACCCACTACGGCCCGGAGACAGTTTGGCTTTGATTGCTCAGACGGAGAATCTGGATCAGAGTTTGCTGCAGGACTATAATCCGGGTGTGAATTTCAGCGGAGGGAGTGGTTACGTGTATATTCCAGGCAAAGAACCAGCAAGGCAGTTGATAGTTTCGAGTTTTGGATCGACCCAGATGAAGTTCCAGATTGGATGTGTGTTGCTTGTTGTGTCGTGTCTTTGTTCTGGGGCGAAGTCCCAGTGCAGTAGTAGCCAAAGCTGCGATGCTCTGGGTTCTTATTACGTGTGGCAGGGCACCAATCTCACCTTCATGGGAACATTAATGGACACCAGCGACACCATCATCGTCAGCTACAACAAAGGGACGGTGCCGAACAAGGACAGTGTTCTATTCGGGACAAGGGTCAATATTCCGTTCAGCTGTGGCTGCTTAAACGGTTCGTTTCTGGGACACATGTTCGAGTACGACGTCATCTCCGGCGACACGTACGACACGGTTGCGAGTAAGTACTATTCCAACCTGACCACGGTGGATGCTATGGCGTGGTTCAATGAGTACCCTGCGAATAACATACCCGATAATGCGAAGCTGAATGTGGTCGTCAACTGTTCGTGTGGGAATAGAGCGGTTTCCAAGGAATATGGGTTGTTTATCACGTACCCACTTCGGCCCGAAGACAGTTTGGCTTCGATTGCTCAGACGGAGAATCTGGATCCGAGTTTGCTGCAGGAGTATAATCCGGGAGTGAACTTTAGCCGAGGGAGTGGCTATGTGTATATTCCAGGCAAAGATCAAAATGGAAGCTATGTGCCTTTGAAGCAAAGTTCAGCTGGACTAGCAGGTGGAGCCATTGCTGGCATAATTGTTGGAGTAATTGCTGGACTGCTGTTAGTGGCTGGTTTTGTATATTTTGGATTTTACCAAAAGAAGAAGGTGGACACAACAGTACTGTTACCAACATCTGAATATCAATCTTCTCCAAATGGGCGCTCCTTTGGAGCTATACCAGATAAATCTACTGAATCCAACAGTGCTGCTAGAGGCCCAACAGGCATTACTGTGGACAAATCAGTGGAATTCTCTTTCGAAGAACTTTCCCGGGCTACTGATAACTTCAGTCTGGCTAATAAGATTGGACAAGGTGGTTTTGGGGCTGTTTACTATGCCGAGCTGAGAGGCGAGAAAGCTGCAATCAAGAAGATGGATATGCAGGCGTCAAAAGAATTTCTTTCCGAATTAAACGTTTTGACACGTGTACATCACTTGAATCTGGTGCGCTTGATTGGTTATTGTGTTGAGGGATCCCTTTTTCTCGTATATGAATTCATTGAAAATGGCAATTTAAGCCAACATCTACGCGGTTCAGGGAGGGACCCAATACCATGGTCTAATAGAGTGCAGATTGCCCTGGATTCAGCCAGAGGTCTTGAATACATTCATGAGCATACTGTTCCTGTTTATATCCACCGTGACATAAAGTCAGCCAATATATTAATTGACAAGAACTACCATGCAAAGGTTGCAGATTTTGGGTTGACTAAACTGACTGAAGTTGGAAGTACATCACTCCCGACGCGTCTTGTGGGGACATTTGGATACATGCCACCAGAATATGCTCAGTATGGCGAAGTTTCTCCCAAAGTAGATGTATATGCTTTTGGAGTTGTCCTTTATGAACTAATCTCTGCCAAGGAAGCAGTTGTGAGGGAAAATGCTTCTGGAGCAGAATCAAGGGGCCTTGTTGGTTTGTTCGAGGAAGTCCTTAATCAGCCCGATCCAAGAGAAGAGCTCCAAAAAGTGGTTGACCCTAGGCTTGGAGACAACTATCCACTTGAATCAGTCCGCAAGATGGCCCAACTTGCCAAAGCATGCGTGCATGAGAATAAAGAATTGCGCCCAAGTATGCGATCTATTGTTGTTGCGTTAATGACACTTTCATCATCAACTGAGGATTGGGATGTCGGATCCTTTTATGAAAATCAAGCTCTTGTCAATCTAATGTCAGGAAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005775 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008061 GO:0008144 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009608 GO:0009609 GO:0009617 GO:0009877 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031974 GO:0036211 GO:0038023 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0097367 GO:0140096 GO:1901564 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

LysM Pkinase Pkinase_Tyr

Protein Analysis

858

Amino Acids

93.45

Weight (kDa)

5.28

Isoelectric Point (pI)

37.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LysM_RLK	PF23577	185 - 228	1e-18	LysM domain
LysM2_CERK1_LYK3_4_5	PF23472	338 - 386	5.4e-06	LYK3/4/5 LysM2 domain
LysM_RLK	PF23577	405 - 448	8.6e-20	LysM domain
PK_Tyr_Ser-Thr	PF07714	561 - 828	2.8e-46	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	561 - 826	5.4e-44	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001281)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G21630
fragaria_vesca	FvH4_3g01490 FvH4_3g01490 FvH4_6g42640 FvH4_6g42640 FvH4_6g42640 FvH4_6g42640 FvH4_6g42640
malus_domestica	MD05G1351500.v1.1 MD09G1111800.v1.1 MD13G1277200.v1.1 MD17G1102100.v1.1
prunus_persica	Prupe.3G213000_v2.0.a1 Prupe.3G213100_v2.0.a1 Prupe.4G016100_v2.0.a1 Prupe.4G016100_v2.0.a1 Prupe.4G016100_v2.0.a1
pyrus_communis	pycom05g31860 pycom09g03640 pycom17g09660
rosa_chinensis	RchiOBHm_Chr2g0158511 RchiOBHm_Chr2g0158521 RchiOBHm_Chr5g0002351 RchiOBHm_Chr7g0236981
rosa_laevigata	RLG00000021092 RLG00000021093 RLG00000031021
rosa_multiflora	Rmu_co8416181.1_g000001 Rmu_co8437957.1_g000001 Rmu_sc0005896.1_g000009 Rmu_sc0034002.1_g000001 Rmu_sc0037966.1_g000001
rosa_roxburghii	Rroxscaffold_1G00073530 Rroxscaffold_2G00091310 Rroxscaffold_2G00091320
rosa_rugosa	Rorug02G0469800 Rorug02G0469800 Rorug04G0395400 Rorug04G0395400
rosa_samantha	Rh2AG535400 Rh2BG548700 Rh2BG548800 Rh2CG519100 Rh2CG519200 Rh2DG558300 Rh2DG558400 Rh5AG018200 Rh5BG020900 Rh5CG020100 Rh5DG018900 Rh7AG456900
rosa_wichuraiana	Rw2G044340 Rw2G044350 Rw5G001710 Rw6G041530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1391
AatII	GACGTC	2 cut(s)	369, 1032
Acc36I	ACCTGC	1 cut(s)	1391
AccB1I	GGYRCC	4 cut(s)	191, 265, 854, 928
AccB7I	CCANNNNNTGG	2 cut(s)	441, 2359
AccBSI	CCGCTC	1 cut(s)	1187
AccI	GTMKAC	1 cut(s)	51
AccII	CGCG	3 cut(s)	527, 1920, 2137
AccIII	TCCGGA	1 cut(s)	81
AciI	CCGC	6 cut(s)	428, 527, 657, 1187, 1920, 2404
AclI	AACGTT	1 cut(s)	1803
AcsI	RAATTY	4 cut(s)	649, 1637, 1784, 1883
AcuI	CTGAAG	2 cut(s)	1660, 2130
AcvI	CACGTG	2 cut(s)	352, 1814
AcyI	GRCGYC	4 cut(s)	192, 366, 1029, 1775
AfiI	CCNNNNNNNGG	7 cut(s)	424, 441, 1087, 2077, 2359, 2375, 2410
AgsI	TTSAA	8 cut(s)	287, 448, 1111, 1378, 1828, 1892, 1991, 2396
AhdI	GACNNNNNGTC	1 cut(s)	2038
AjnI	CCWGG	3 cut(s)	682, 1342, 1968
AjuI	GAANNNNNNNTTGG	2 cut(s)	851, 883
Alw21I	GWGCWC	1 cut(s)	2352
Alw26I	GTCTC	3 cut(s)	376, 570, 2373
Ama87I	CYCGRG	1 cut(s)	1659
Aor13HI	TCCGGA	1 cut(s)	81
AoxI	GGCC	6 cut(s)	346, 569, 1229, 1603, 2296, 2412
ApeKI	GCWGC	8 cut(s)	156, 324, 625, 819, 987, 1285, 1595, 1748
ApoI	RAATTY	4 cut(s)	649, 1637, 1784, 1883
AseI	ATTAAT	2 cut(s)	881, 2054
Asp700I	GAANNNNTTC	2 cut(s)	339, 1995
AspA2I	CCTAGG	1 cut(s)	2369
AspLEI	GCGC	6 cut(s)	194, 233, 527, 1551, 1839, 2457
AspS9I	GGNCC	7 cut(s)	346, 570, 1230, 1604, 1933, 2296, 2413
AsuC2I	CCSGG	6 cut(s)	345, 573, 642, 1302, 1660, 1661
AsuHPI	GGTGA	2 cut(s)	193, 856
AsuII	TTCGAA	1 cut(s)	1647
AvaI	CYCGRG	1 cut(s)	1659
AvaII	GGWCC	1 cut(s)	1933
AvrII	CCTAGG	1 cut(s)	2369
BaeGI	GKGCMC	1 cut(s)	857
BamHI	GGATCC	3 cut(s)	1273, 1862, 2525
BanI	GGYRCC	4 cut(s)	191, 265, 854, 928
BanII	GRGCYC	1 cut(s)	2352
BbrPI	CACGTG	2 cut(s)	352, 1814
BbsI	GAAGAC	1 cut(s)	1242
Bbv12I	GWGCWC	1 cut(s)	2352
BbvI	GCAGC	8 cut(s)	143, 311, 612, 806, 974, 1272, 1582, 1735
BccI	CCATC	4 cut(s)	60, 908, 1756, 2404
BceAI	ACGGC	2 cut(s)	441, 584
BcgI	CGANNNNNNTGC	8 cut(s)	377, 411, 1040, 1074, 1267, 1301, 1730, 1764
BciT130I	CCWGG	3 cut(s)	684, 1344, 1970
BciVI	GTATCC	1 cut(s)	2150
BcnI	CCSGG	6 cut(s)	345, 573, 642, 1302, 1660, 1661
BcoDI	GTCTC	3 cut(s)	376, 570, 2373
BfaI	CTAG	3 cut(s)	1397, 1599, 2370
BfmI	CTRYAG	2 cut(s)	626, 1286
BfoI	RGCGCY	3 cut(s)	195, 234, 1552
BfuAI	ACCTGC	1 cut(s)	1391
BfuI	GTATCC	1 cut(s)	2150
BglI	GCCNNNNNGGC	1 cut(s)	1611
BisI	GCNGC	8 cut(s)	157, 325, 626, 820, 988, 1286, 1596, 1749
BlnI	CCTAGG	1 cut(s)	2369
BlsI	GCNGC	8 cut(s)	158, 326, 627, 821, 989, 1287, 1597, 1750
BmcAI	AGTACT	3 cut(s)	407, 1070, 1509
Bme1390I	CCNGG	9 cut(s)	345, 573, 642, 684, 1302, 1344, 1660, 1661, 1970
Bme18I	GGWCC	1 cut(s)	1933
BmeRI	GACNNNNNGTC	1 cut(s)	2038
BmeT110I	CYCGRG	1 cut(s)	1659
BmgT120I	GGNCC	7 cut(s)	346, 570, 1230, 1604, 1933, 2296, 2413
BmrFI	CCNGG	9 cut(s)	345, 573, 642, 684, 1302, 1344, 1660, 1661, 1970
BmrI	ACTGGG	2 cut(s)	130, 793
BmsI	GCATC	4 cut(s)	151, 421, 814, 1084
BmuI	ACTGGG	2 cut(s)	130, 793
BoxI	GACNNNNGTC	1 cut(s)	962
BpiI	GAAGAC	1 cut(s)	1242
BplI	GAGNNNNNCTC	2 cut(s)	2334, 2366
BpmI	CTGGAG	2 cut(s)	144, 2301
Bpu14I	TTCGAA	1 cut(s)	1647
BpuMI	CCSGG	6 cut(s)	345, 573, 642, 1302, 1660, 1661
Bsa29I	ATCGAT	1 cut(s)	20
BsaAI	YACGTR	8 cut(s)	352, 384, 558, 673, 844, 1047, 1218, 1814
BsaBI	GATNNNNATC	1 cut(s)	32
BsaHI	GRCGYC	4 cut(s)	192, 366, 1029, 1775
BsaWI	WCCGGW	1 cut(s)	81
BsaXI	ACNNNNNCTCC	4 cut(s)	356, 386, 1019, 1049
Bsc4I	CCNNNNNNNGG	7 cut(s)	424, 441, 1087, 2077, 2359, 2375, 2410
Bse1I	ACTGG	2 cut(s)	136, 799
Bse3DI	GCAATG	1 cut(s)	1410
Bse8I	GATNNNNATC	1 cut(s)	32
BseAI	TCCGGA	1 cut(s)	81
BseBI	CCWGG	3 cut(s)	684, 1344, 1970
BseCI	ATCGAT	1 cut(s)	20
BseGI	GGATG	5 cut(s)	95, 436, 758, 1099, 2524
BseJI	GATNNNNATC	1 cut(s)	32
BseLI	CCNNNNNNNGG	7 cut(s)	424, 441, 1087, 2077, 2359, 2375, 2410
BseMI	GCAATG	1 cut(s)	1410
BseMII	CTCAG	5 cut(s)	611, 1271, 1724, 2192, 2499
BseNI	ACTGG	2 cut(s)	136, 799
BseSI	GKGCMC	1 cut(s)	857
BseXI	GCAGC	8 cut(s)	143, 311, 612, 806, 974, 1272, 1582, 1735
BsgI	GTGCAG	3 cut(s)	160, 823, 1979
Bsh1236I	CGCG	3 cut(s)	527, 1920, 2137
BshFI	GGCC	6 cut(s)	348, 571, 1231, 1605, 2298, 2414
BshNI	GGYRCC	4 cut(s)	191, 265, 854, 928
BshVI	ATCGAT	1 cut(s)	20
BsiHKAI	GWGCWC	1 cut(s)	2352
BsiHKCI	CYCGRG	1 cut(s)	1659
BsiSI	CCGG	8 cut(s)	82, 344, 375, 573, 641, 1038, 1301, 1660
BsiWI	CGTACG	2 cut(s)	384, 1047
BslFI	GGGAC	7 cut(s)	118, 781, 937, 970, 1023, 1946, 2161
BslI	CCNNNNNNNGG	7 cut(s)	424, 441, 1087, 2077, 2359, 2375, 2410
BsmAI	GTCTC	3 cut(s)	376, 570, 2373
BsmBI	CGTCTC	1 cut(s)	376
BsmFI	GGGAC	7 cut(s)	118, 781, 937, 970, 1023, 1946, 2161
BsnI	GGCC	6 cut(s)	348, 571, 1231, 1605, 2298, 2414
BsoBI	CYCGRG	1 cut(s)	1659
Bsp119I	TTCGAA	1 cut(s)	1647
Bsp1286I	GDGCHC	2 cut(s)	857, 2352
Bsp13I	TCCGGA	1 cut(s)	81
Bsp1407I	TGTACA	1 cut(s)	1816
Bsp19I	CCATGG	2 cut(s)	435, 1943
BspACI	CCGC	6 cut(s)	428, 527, 657, 1187, 1920, 2404
BspANI	GGCC	6 cut(s)	348, 571, 1231, 1605, 2298, 2414
BspCNI	CTCAG	5 cut(s)	610, 1270, 1725, 2191, 2500
BspDI	ATCGAT	1 cut(s)	20
BspEI	TCCGGA	1 cut(s)	81
BspFNI	CGCG	3 cut(s)	527, 1920, 2137
BspHI	TCATGA	1 cut(s)	1999
BspMAI	CTGCAG	2 cut(s)	630, 1290
BspMI	ACCTGC	1 cut(s)	1391
BspQI	GCTCTTC	1 cut(s)	2340
BspT104I	TTCGAA	1 cut(s)	1647
BspT107I	GGYRCC	4 cut(s)	191, 265, 854, 928
BsrBI	CCGCTC	1 cut(s)	1187
BsrDI	GCAATG	1 cut(s)	1410
BsrGI	TGTACA	1 cut(s)	1816
BsrI	ACTGG	2 cut(s)	136, 799
BssNAI	GTATAC	1 cut(s)	52
BssNI	GRCGYC	4 cut(s)	192, 366, 1029, 1775
BssT1I	CCWWGG	5 cut(s)	435, 1194, 1943, 2250, 2369
Bst1107I	GTATAC	1 cut(s)	52
Bst2UI	CCWGG	3 cut(s)	684, 1344, 1970
Bst6I	CTCTTC	1 cut(s)	2340
BstACI	GRCGYC	4 cut(s)	192, 366, 1029, 1775
BstAPI	GCANNNNNTGC	2 cut(s)	156, 2081
BstAUI	TGTACA	1 cut(s)	1816
BstBAI	YACGTR	8 cut(s)	352, 384, 558, 673, 844, 1047, 1218, 1814
BstBI	TTCGAA	1 cut(s)	1647
BstC8I	GCNNGC	5 cut(s)	247, 1417, 1773, 2431, 2435
BstDEI	CTNAG	5 cut(s)	597, 1257, 1733, 2178, 2508
BstDSI	CCRYGG	4 cut(s)	423, 435, 1086, 1943
BstF5I	GGATG	5 cut(s)	95, 436, 758, 1099, 2524
BstFNI	CGCG	3 cut(s)	527, 1920, 2137
BstH2I	RGCGCY	3 cut(s)	195, 234, 1552
BstHHI	GCGC	6 cut(s)	194, 233, 527, 1551, 1839, 2457
BstMAI	GTCTC	3 cut(s)	376, 570, 2373
BstNI	CCWGG	3 cut(s)	684, 1344, 1970
BstNSI	RCATGY	4 cut(s)	184, 1018, 2164, 2433
BstPAI	GACNNNNGTC	1 cut(s)	962
BstSCI	CCNGG	9 cut(s)	343, 571, 640, 682, 1300, 1342, 1658, 1659, 1968
BstSFI	CTRYAG	2 cut(s)	626, 1286
BstSLI	GKGCMC	1 cut(s)	857
BstUI	CGCG	3 cut(s)	527, 1920, 2137
BstV1I	GCAGC	8 cut(s)	143, 311, 612, 806, 974, 1272, 1582, 1735
BstV2I	GAAGAC	1 cut(s)	1242
BstX2I	RGATCY	3 cut(s)	1273, 1862, 2525
BstXI	CCANNNNNNTGG	1 cut(s)	1944
BstYI	RGATCY	3 cut(s)	1273, 1862, 2525
BstZ17I	GTATAC	1 cut(s)	52
Bsu15I	ATCGAT	1 cut(s)	20
BsuI	GTATCC	1 cut(s)	2150
BsuRI	GGCC	6 cut(s)	348, 571, 1231, 1605, 2298, 2414
BsuTUI	ATCGAT	1 cut(s)	20
BtgI	CCRYGG	4 cut(s)	423, 435, 1086, 1943
BtgZI	GCGATG	3 cut(s)	174, 226, 837
BtsCI	GGATG	5 cut(s)	95, 436, 758, 1099, 2524
BtsIMutI	CAGTG	6 cut(s)	143, 286, 806, 949, 1597, 1638
BveI	ACCTGC	1 cut(s)	1391
Cac8I	GCNNGC	5 cut(s)	247, 1417, 1773, 2431, 2435
CciI	TCATGA	1 cut(s)	1999
CfoI	GCGC	6 cut(s)	194, 233, 527, 1551, 1839, 2457
Cfr13I	GGNCC	7 cut(s)	346, 570, 1230, 1604, 1933, 2296, 2413
Cfr9I	CCCGGG	1 cut(s)	1659
ClaI	ATCGAT	1 cut(s)	20
CseI	GACGC	3 cut(s)	1764, 2126, 2143
DdeI	CTNAG	5 cut(s)	597, 1257, 1733, 2178, 2508
DinI	GGCGCC	1 cut(s)	193
DriI	GACNNNNNGTC	1 cut(s)	2038
Eam1104I	CTCTTC	1 cut(s)	2340
Eam1105I	GACNNNNNGTC	1 cut(s)	2038
EarI	CTCTTC	1 cut(s)	2340
EciI	GGCGGA	1 cut(s)	443
Ecl136II	GAGCTC	1 cut(s)	2350
Eco130I	CCWWGG	5 cut(s)	435, 1194, 1943, 2250, 2369
Eco24I	GRGCYC	1 cut(s)	2352
Eco47I	GGWCC	1 cut(s)	1933
Eco53kI	GAGCTC	1 cut(s)	2350
Eco57I	CTGAAG	2 cut(s)	1660, 2130
Eco72I	CACGTG	2 cut(s)	352, 1814
Eco88I	CYCGRG	1 cut(s)	1659
EcoICRI	GAGCTC	1 cut(s)	2350
EcoO109I	RGGNCCY	2 cut(s)	1933, 2296
EcoRI	GAATTC	2 cut(s)	1637, 1883
EcoRII	CCWGG	3 cut(s)	682, 1342, 1968
EcoT14I	CCWWGG	5 cut(s)	435, 1194, 1943, 2250, 2369
EcoT38I	GRGCYC	1 cut(s)	2352
EgeI	GGCGCC	1 cut(s)	193
EheI	GGCGCC	1 cut(s)	193
ErhI	CCWWGG	5 cut(s)	435, 1194, 1943, 2250, 2369
Esp3I	CGTCTC	1 cut(s)	376
FalI	AAGNNNNNCTT	2 cut(s)	1773, 1805
FaqI	GGGAC	7 cut(s)	118, 781, 937, 970, 1023, 1946, 2161
FblI	GTMKAC	1 cut(s)	51
Fnu4HI	GCNGC	8 cut(s)	157, 325, 626, 820, 988, 1286, 1596, 1749
FokI	GGATG	5 cut(s)	102, 443, 765, 1106, 2531
FriOI	GRGCYC	1 cut(s)	2352
Fsp4HI	GCNGC	8 cut(s)	157, 325, 626, 820, 988, 1286, 1596, 1749
FspBI	CTAG	3 cut(s)	1397, 1599, 2370
GlaI	GCGC	6 cut(s)	193, 232, 526, 1550, 1838, 2456
GluI	GCNGC	8 cut(s)	157, 325, 626, 820, 988, 1286, 1596, 1749
GsuI	CTGGAG	2 cut(s)	144, 2301
HaeII	RGCGCY	3 cut(s)	195, 234, 1552
HaeIII	GGCC	6 cut(s)	348, 571, 1231, 1605, 2298, 2414
HapII	CCGG	8 cut(s)	82, 344, 375, 573, 641, 1038, 1301, 1660
HgaI	GACGC	3 cut(s)	1764, 2126, 2143
HhaI	GCGC	6 cut(s)	194, 233, 527, 1551, 1839, 2457
Hin1I	GRCGYC	4 cut(s)	192, 366, 1029, 1775
Hin6I	GCGC	6 cut(s)	192, 231, 525, 1549, 1837, 2455
HinP1I	GCGC	6 cut(s)	192, 231, 525, 1549, 1837, 2455
HincII	GTYRAC	4 cut(s)	505, 1165, 2097, 2365
HindII	GTYRAC	4 cut(s)	505, 1165, 2097, 2365
HinfI	GANTC	9 cut(s)	131, 335, 607, 1267, 1583, 1828, 1973, 2288, 2396
HpaII	CCGG	8 cut(s)	82, 344, 375, 573, 641, 1038, 1301, 1660
HphI	GGTGA	2 cut(s)	193, 856
Hpy99I	CGWCG	6 cut(s)	212, 265, 368, 371, 1031, 2137
HpyAV	CCTTC	4 cut(s)	214, 877, 1486, 2562
HpyF3I	CTNAG	5 cut(s)	597, 1257, 1733, 2178, 2508
Hsp92I	GRCGYC	4 cut(s)	192, 366, 1029, 1775
HspAI	GCGC	6 cut(s)	192, 231, 525, 1549, 1837, 2455
KasI	GGCGCC	1 cut(s)	191
KflI	GGGWCCC	1 cut(s)	1933
Kpn2I	TCCGGA	1 cut(s)	81
LguI	GCTCTTC	1 cut(s)	2340
LmnI	GCTCC	6 cut(s)	125, 1406, 1556, 1559, 2282, 2355
Lsp1109I	GCAGC	8 cut(s)	143, 311, 612, 806, 974, 1272, 1582, 1735
LweI	GCATC	4 cut(s)	151, 421, 814, 1084
MaeI	CTAG	3 cut(s)	1397, 1599, 2370
MaeIII	GTNAC	3 cut(s)	668, 1512, 2030
MbiI	CCGCTC	1 cut(s)	1187
MboII	GAAGA	7 cut(s)	82, 1247, 1501, 1527, 1661, 1771, 2357
MflI	RGATCY	3 cut(s)	1273, 1862, 2525
MhlI	GDGCHC	2 cut(s)	857, 2352
MluI	ACGCGT	1 cut(s)	2135
Mly113I	GGCGCC	1 cut(s)	192
MlyI	GAGTC	1 cut(s)	140
MmeI	TCCRAC	6 cut(s)	190, 1101, 1408, 1611, 2095, 2503
MroI	TCCGGA	1 cut(s)	81
MroXI	GAANNNNTTC	2 cut(s)	339, 1995
MseI	TTAA	7 cut(s)	881, 992, 1799, 1904, 2054, 2325, 2486
MslI	CAYNNNNRTG	2 cut(s)	869, 1619
MspA1I	CMGCKG	4 cut(s)	318, 657, 981, 1391
MspI	CCGG	8 cut(s)	82, 344, 375, 573, 641, 1038, 1301, 1660
MspR9I	CCNGG	9 cut(s)	345, 573, 642, 684, 1302, 1344, 1660, 1661, 1970
MvaI	CCWGG	3 cut(s)	684, 1344, 1970
MvnI	CGCG	3 cut(s)	527, 1920, 2137
NarI	GGCGCC	1 cut(s)	192
NciI	CCSGG	6 cut(s)	345, 573, 642, 1302, 1660, 1661
NcoI	CCATGG	2 cut(s)	435, 1943
NmeAIII	GCCGAG	2 cut(s)	1343, 1753
NmuCI	GTSAC	1 cut(s)	2030
NspI	RCATGY	4 cut(s)	184, 1018, 2164, 2433
NspV	TTCGAA	1 cut(s)	1647
PaeI	GCATGC	1 cut(s)	2433
PagI	TCATGA	1 cut(s)	1999
PaqCI	CACCTGC	1 cut(s)	1391
PciI	ACATGT	2 cut(s)	180, 1014
PciSI	GCTCTTC	1 cut(s)	2340
PcsI	WCGNNNNNNNCGW	3 cut(s)	363, 375, 1026
PdmI	GAANNNNTTC	2 cut(s)	339, 1995
PfeI	GAWTC	8 cut(s)	335, 607, 1267, 1583, 1828, 1973, 2288, 2396
Pfl23II	CGTACG	2 cut(s)	384, 1047
PflFI	GACNNNGTC	1 cut(s)	368
PflMI	CCANNNNNTGG	2 cut(s)	441, 2359
PfoI	TCCNGGA	1 cut(s)	1300
PkrI	GCNGC	8 cut(s)	158, 326, 627, 821, 989, 1287, 1597, 1750
PleI	GAGTC	1 cut(s)	139
PluTI	GGCGCC	1 cut(s)	195
PmaCI	CACGTG	2 cut(s)	352, 1814
PmlI	CACGTG	2 cut(s)	352, 1814
PpsI	GAGTC	1 cut(s)	139
Ppu21I	YACGTR	8 cut(s)	352, 384, 558, 673, 844, 1047, 1218, 1814
PpuMI	RGGWCCY	1 cut(s)	1933
PscI	ACATGT	2 cut(s)	180, 1014
PshAI	GACNNNNGTC	1 cut(s)	962
PshBI	ATTAAT	2 cut(s)	881, 2054
Psp124BI	GAGCTC	1 cut(s)	2352
Psp1406I	AACGTT	1 cut(s)	1803
Psp5II	RGGWCCY	1 cut(s)	1933
Psp6I	CCWGG	3 cut(s)	682, 1342, 1968
PspCI	CACGTG	2 cut(s)	352, 1814
PspGI	CCWGG	3 cut(s)	682, 1342, 1968
PspLI	CGTACG	2 cut(s)	384, 1047
PspPI	GGNCC	7 cut(s)	346, 570, 1230, 1604, 1933, 2296, 2413
PspPPI	RGGWCCY	1 cut(s)	1933
PstI	CTGCAG	2 cut(s)	630, 1290
PsuI	RGATCY	3 cut(s)	1273, 1862, 2525
PsyI	GACNNNGTC	1 cut(s)	368
PvuII	CAGCTG	3 cut(s)	318, 981, 1391
RseI	CAYNNNNRTG	2 cut(s)	869, 1619
SacI	GAGCTC	1 cut(s)	2352
SapI	GCTCTTC	1 cut(s)	2340
SaqAI	TTAA	7 cut(s)	881, 992, 1799, 1904, 2054, 2325, 2486
SatI	GCNGC	8 cut(s)	157, 325, 626, 820, 988, 1286, 1596, 1749
Sau96I	GGNCC	7 cut(s)	346, 570, 1230, 1604, 1933, 2296, 2413
ScaI	AGTACT	3 cut(s)	407, 1070, 1509
SchI	GAGTC	1 cut(s)	140
ScrFI	CCNGG	9 cut(s)	345, 573, 642, 684, 1302, 1344, 1660, 1661, 1970
SduI	GDGCHC	2 cut(s)	857, 2352
SfaNI	GCATC	4 cut(s)	151, 421, 814, 1084
SfcI	CTRYAG	2 cut(s)	626, 1286
SfoI	GGCGCC	1 cut(s)	193
SfuI	TTCGAA	1 cut(s)	1647
SinI	GGWCC	1 cut(s)	1933
SmaI	CCCGGG	1 cut(s)	1661
SmiMI	CAYNNNNRTG	2 cut(s)	869, 1619
SphI	GCATGC	1 cut(s)	2433
SsiI	CCGC	6 cut(s)	428, 527, 657, 1187, 1920, 2404
SspDI	GGCGCC	1 cut(s)	191
SspI	AATATT	1 cut(s)	970
SspMI	CTAG	3 cut(s)	1397, 1599, 2370
SstI	GAGCTC	1 cut(s)	2352
StyD4I	CCNGG	9 cut(s)	343, 571, 640, 682, 1300, 1342, 1658, 1659, 1968
StyI	CCWWGG	5 cut(s)	435, 1194, 1943, 2250, 2369
TaqI	TCGA	9 cut(s)	8, 20, 357, 716, 728, 1020, 1250, 1647, 2313
TatI	WGTACW	5 cut(s)	405, 1068, 1507, 1816, 2120
TfiI	GAWTC	8 cut(s)	335, 607, 1267, 1583, 1828, 1973, 2288, 2396
Tru1I	TTAA	7 cut(s)	881, 992, 1799, 1904, 2054, 2325, 2486
Tru9I	TTAA	7 cut(s)	881, 992, 1799, 1904, 2054, 2325, 2486
TscAI	CASTG	6 cut(s)	143, 286, 806, 949, 1597, 1638
TseFI	GTSAC	1 cut(s)	2030
TseI	GCWGC	8 cut(s)	156, 324, 625, 819, 987, 1285, 1595, 1748
Tsp45I	GTSAC	1 cut(s)	2030
TspGWI	ACGGA	4 cut(s)	300, 617, 963, 1277
TspMI	CCCGGG	1 cut(s)	1659
TspRI	CASTG	6 cut(s)	143, 286, 806, 949, 1597, 1638
Tth111I	GACNNNGTC	1 cut(s)	368
Van91I	CCANNNNNTGG	2 cut(s)	441, 2359
VpaK11BI	GGWCC	1 cut(s)	1933
VspI	ATTAAT	2 cut(s)	881, 2054
XapI	RAATTY	4 cut(s)	649, 1637, 1784, 1883
XceI	RCATGY	4 cut(s)	184, 1018, 2164, 2433
XmaI	CCCGGG	1 cut(s)	1659
XmaJI	CCTAGG	1 cut(s)	2369
XmiI	GTMKAC	1 cut(s)	51
XmnI	GAANNNNTTC	2 cut(s)	339, 1995
XspI	CTAG	3 cut(s)	1397, 1599, 2370
ZraI	GACGTC	2 cut(s)	367, 1030
ZrmI	AGTACT	3 cut(s)	407, 1070, 1509

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.