Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0001281

Physical Location & Seq

Genomic Coordinates Forward (+)

1300695 .. 1305217

Gene Structure 4523 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG018200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1440 bp

ATGATGCTTGCAGAAGGAAACACACAAATCAAATTGTTCGCCTTCCATGTATTTCTTCTTCTTCTATCATTCACAGCCAAAGCTAAGTGCAAACATGGCTGCCTTGCCTTAGCCTCATACAATGTCTGGGATGGTTCAAACCTCACTTACATTAGCTACATCTTTAGCCAGCAAATCCCTGAAATTCTCAAGTACAACCCTCAAGTCAAGAACTCTGATAGCCTCCAAACTGGTACTCGAATCAACATACCATTTTCTTGTGACTGCTTGAATAGTGATTTTTTGGGACATACGTTCACCTACCTCACCCAATATAGTGATACCTATTACAAAGTTGCCCAGATCGCATTTGCTAATCTCACTACAGAAGAATGGGTGCATAGAGTGAACACTTACACACCAACTCAGATACCTGACAAAGCTCCAATTAACGTAACAGTGAACTGCTCTTGCGGTGATGGAAAGATATCAAAGGATTATGGGTTTTTCTTGACATATCCTCTTCATCCCGGTGAGGGCTTGTCCTCTGTGGCTGCGGAGAGTGGTGTTCCAGCAAGGTTGCTGGAGATGTACAACAAAGTGTCCAACTTTGGTTCAGGCAATGACTTAGTCTTTGTGCCGGCAAGAGATCAAAATGGGACTTTTCCACCATATAAGTCAAGAACAGCTGGTATCTCAAGAGGAGCTATTATTGGTATATGTGCTGGAGGAGCTTCTGCTGCTCTAGCTTTGGCAATTTTCCTATTTGCTTGGGTTTATAGGAGGAAGGTGGTTGAGGCACCATTTCTTTCCACACCGTCTGAAGAGCGCTATCTTCAAATTCAACATACAAATGTTTTAACTTCTGGAATTACTTCTGAGACAACTTCTGATACAGTTGCTCTAGTTAGCGGTTCTTCTCCGGGCCTTATGGGTATAACTGTGGACAAATCAGTGGAGTTCTCATATGAAGAGCTATCCAAAGCTACTAATGACTTCAGCATAAAAATTGGACAGGGTGGGTTTGGAGCTGTTTATTATGCAGAACTCCGAGGCGAGAAAGCAGCAATCAAAAGGATGGATATGCAAGCAACAAAAGAATTCCTTGCTGAACTGAAGGTTCTAACACATGTTCATCACTTAAATCTGGTGCGCTTGATAGGGTATTGTGTCGAGGACTCCTTGTTCCTAGTGTATGAGTACATTGAAAATGGTAACTTAAGTCAGCATTTGCATGGCTCTTCTGGTCTAGGCCCATTGCCATGGCAAACCAGGATGCAAATAGCCTTAGATTCTGCAAGAGGACTAGAATATATCCACGAACACACTGTTCCTGTCTACATTCATCGTGACATCAAGTCAGCAAACATCTTAATAGACAAAAACTTCAGCGCAAAGGTTGTGAAAAAATGCCTTTATTATTACTTTTGGCTTGAATTTTTTCTAAATATAAACATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

LysM Pkinase Pkinase_Tyr

Protein Analysis

479

Amino Acids

53.31

Weight (kDa)

6.7

Isoelectric Point (pI)

27.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LysM_RLK	PF23577	164 - 207	3e-14	LysM domain
PK_Tyr_Ser-Thr	PF07714	328 - 461	2.8e-26	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	329 - 460	5.3e-22	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001281)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G21630
fragaria_vesca	FvH4_3g01490 FvH4_3g01490 FvH4_6g42640 FvH4_6g42640 FvH4_6g42640 FvH4_6g42640 FvH4_6g42640
malus_domestica	MD05G1351500.v1.1 MD09G1111800.v1.1 MD13G1277200.v1.1 MD17G1102100.v1.1
prunus_persica	Prupe.3G213000_v2.0.a1 Prupe.3G213100_v2.0.a1 Prupe.4G016100_v2.0.a1 Prupe.4G016100_v2.0.a1 Prupe.4G016100_v2.0.a1
pyrus_communis	pycom05g31860 pycom09g03640 pycom17g09660
rosa_chinensis	RchiOBHm_Chr2g0158511 RchiOBHm_Chr2g0158521 RchiOBHm_Chr5g0002351 RchiOBHm_Chr7g0236981
rosa_laevigata	RLG00000021092 RLG00000021093 RLG00000031021
rosa_multiflora	Rmu_co8416181.1_g000001 Rmu_co8437957.1_g000001 Rmu_sc0005896.1_g000009 Rmu_sc0034002.1_g000001 Rmu_sc0037966.1_g000001
rosa_roxburghii	Rroxscaffold_1G00073530 Rroxscaffold_2G00091310 Rroxscaffold_2G00091320
rosa_rugosa	Rorug02G0469800 Rorug02G0469800 Rorug04G0395400 Rorug04G0395400
rosa_samantha	Rh2AG535400 Rh2BG548700 Rh2BG548800 Rh2CG519100 Rh2CG519200 Rh2DG558300 Rh2DG558400 Rh5AG018200 Rh5BG020900 Rh5CG020100 Rh5DG018900 Rh7AG456900
rosa_wichuraiana	Rw2G044340 Rw2G044350 Rw5G001710 Rw6G041530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	778
AccI	GTMKAC	1 cut(s)	1317
AciI	CCGC	3 cut(s)	453, 536, 891
AcsI	RAATTY	4 cut(s)	183, 819, 1079, 1415
AcuI	CTGAAG	4 cut(s)	822, 961, 1115, 1351
AfaI	GTAC	4 cut(s)	194, 235, 572, 1181
AfeI	AGCGCT	1 cut(s)	809
AfiI	CCNNNNNNNGG	1 cut(s)	515
AflII	CTTAAG	1 cut(s)	1198
AflIII	ACRYGT	1 cut(s)	1108
AgsI	TTSAA	6 cut(s)	138, 271, 818, 824, 1187, 1415
AhdI	GACNNNNNGTC	1 cut(s)	1336
AjnI	CCWGG	1 cut(s)	1250
AloI	GAACNNNNNNTCC	2 cut(s)	1148, 1180
Alw26I	GTCTC	1 cut(s)	854
Aor51HI	AGCGCT	1 cut(s)	809
AoxI	GGCC	2 cut(s)	904, 1231
ApeKI	GCWGC	4 cut(s)	99, 533, 719, 1043
ApoI	RAATTY	4 cut(s)	183, 819, 1079, 1415
ArsI	GACNNNNNNTTYG	2 cut(s)	596, 628
Asp700I	GAANNNNTTC	2 cut(s)	853, 1419
AspLEI	GCGC	3 cut(s)	810, 1134, 1373
AspS9I	GGNCC	2 cut(s)	904, 1232
AsuC2I	CCSGG	2 cut(s)	510, 903
AsuHPI	GGTGA	4 cut(s)	289, 298, 467, 524
BanI	GGYRCC	1 cut(s)	778
BarI	GAAGNNNNNNTAC	2 cut(s)	42, 74
BbvI	GCAGC	4 cut(s)	86, 520, 706, 1055
BccI	CCATC	3 cut(s)	125, 452, 1051
BciT130I	CCWGG	1 cut(s)	1252
BcnI	CCSGG	2 cut(s)	510, 903
BcoDI	GTCTC	1 cut(s)	854
BfaI	CTAG	5 cut(s)	725, 884, 1169, 1229, 1286
BfmI	CTRYAG	1 cut(s)	363
BfoI	RGCGCY	1 cut(s)	811
BfrI	CTTAAG	1 cut(s)	1198
BisI	GCNGC	4 cut(s)	100, 534, 720, 1044
BlsI	GCNGC	4 cut(s)	101, 535, 721, 1045
Bme1390I	CCNGG	3 cut(s)	510, 903, 1252
BmeRI	GACNNNNNGTC	1 cut(s)	1336
BmgT120I	GGNCC	2 cut(s)	904, 1232
BmiI	GGNNCC	1 cut(s)	780
BmrFI	CCNGG	3 cut(s)	510, 903, 1252
BmsI	GCATC	1 cut(s)	1245
BpmI	CTGGAG	2 cut(s)	584, 726
Bpu10I	CCTNAGC	1 cut(s)	109
BpuEI	CTTGAG	3 cut(s)	173, 186, 661
BpuMI	CCSGG	2 cut(s)	510, 903
BsaJI	CCNNGG	2 cut(s)	1030, 1241
Bsc4I	CCNNNNNNNGG	1 cut(s)	515
Bse118I	RCCGGY	1 cut(s)	619
Bse1I	ACTGG	1 cut(s)	235
Bse3DI	GCAATG	2 cut(s)	607, 1235
BseBI	CCWGG	1 cut(s)	1252
BseDI	CCNNGG	2 cut(s)	1030, 1241
BseGI	GGATG	4 cut(s)	136, 505, 1062, 1260
BseLI	CCNNNNNNNGG	1 cut(s)	515
BseMI	GCAATG	2 cut(s)	607, 1235
BseMII	CTCAG	2 cut(s)	419, 849
BseNI	ACTGG	1 cut(s)	235
BseRI	GAGGAG	2 cut(s)	696, 723
BseXI	GCAGC	4 cut(s)	86, 520, 706, 1055
BshFI	GGCC	2 cut(s)	906, 1233
BshNI	GGYRCC	1 cut(s)	778
BsiSI	CCGG	3 cut(s)	510, 620, 902
BslFI	GGGAC	2 cut(s)	300, 652
BslI	CCNNNNNNNGG	1 cut(s)	515
BsmAI	GTCTC	1 cut(s)	854
BsmFI	GGGAC	2 cut(s)	300, 652
BsnI	GGCC	2 cut(s)	906, 1233
Bsp1407I	TGTACA	1 cut(s)	570
Bsp143I	GATC	2 cut(s)	342, 628
Bsp19I	CCATGG	1 cut(s)	1241
BspACI	CCGC	3 cut(s)	453, 536, 891
BspANI	GGCC	2 cut(s)	906, 1233
BspCNI	CTCAG	2 cut(s)	418, 850
BspLI	GGNNCC	1 cut(s)	780
BspQI	GCTCTTC	3 cut(s)	798, 945, 1225
BspT107I	GGYRCC	1 cut(s)	778
BspTI	CTTAAG	1 cut(s)	1198
BsrDI	GCAATG	2 cut(s)	607, 1235
BsrFI	RCCGGY	1 cut(s)	619
BsrGI	TGTACA	1 cut(s)	570
BsrI	ACTGG	1 cut(s)	235
BssAI	RCCGGY	1 cut(s)	619
BssECI	CCNNGG	2 cut(s)	1030, 1241
BssMI	GATC	2 cut(s)	342, 628
BssT1I	CCWWGG	1 cut(s)	1241
Bst2UI	CCWGG	1 cut(s)	1252
Bst4CI	ACNGT	5 cut(s)	439, 798, 877, 922, 1309
Bst6I	CTCTTC	4 cut(s)	507, 798, 945, 1225
BstAFI	CTTAAG	1 cut(s)	1198
BstAUI	TGTACA	1 cut(s)	570
BstC8I	GCNNGC	4 cut(s)	9, 170, 621, 1068
BstDEI	CTNAG	6 cut(s)	84, 109, 405, 607, 858, 1267
BstDSI	CCRYGG	1 cut(s)	1241
BstF5I	GGATG	4 cut(s)	136, 505, 1062, 1260
BstH2I	RGCGCY	1 cut(s)	811
BstHHI	GCGC	3 cut(s)	810, 1134, 1373
BstKTI	GATC	2 cut(s)	345, 631
BstMAI	GTCTC	1 cut(s)	854
BstMBI	GATC	2 cut(s)	342, 628
BstMWI	GCNNNNNNNGC	5 cut(s)	96, 344, 710, 719, 725
BstNI	CCWGG	1 cut(s)	1252
BstNSI	RCATGY	1 cut(s)	1112
BstSCI	CCNGG	3 cut(s)	508, 901, 1250
BstSFI	CTRYAG	1 cut(s)	363
BstV1I	GCAGC	4 cut(s)	86, 520, 706, 1055
BstXI	CCANNNNNNTGG	1 cut(s)	1242
BsuRI	GGCC	2 cut(s)	906, 1233
BtgI	CCRYGG	1 cut(s)	1241
BtsCI	GGATG	4 cut(s)	136, 505, 1062, 1260
BtsIMutI	CAGTG	3 cut(s)	444, 939, 1305
Cac8I	GCNNGC	4 cut(s)	9, 170, 621, 1068
CfoI	GCGC	3 cut(s)	810, 1134, 1373
Cfr10I	RCCGGY	1 cut(s)	619
Cfr13I	GGNCC	2 cut(s)	904, 1232
Csp6I	GTAC	4 cut(s)	193, 234, 571, 1180
CviAII	CATG	5 cut(s)	47, 95, 1109, 1214, 1242
CviQI	GTAC	4 cut(s)	193, 234, 571, 1180
DdeI	CTNAG	6 cut(s)	84, 109, 405, 607, 858, 1267
DpnI	GATC	2 cut(s)	344, 630
DpnII	GATC	2 cut(s)	342, 628
DriI	GACNNNNNGTC	1 cut(s)	1336
Eam1104I	CTCTTC	4 cut(s)	507, 798, 945, 1225
Eam1105I	GACNNNNNGTC	1 cut(s)	1336
EarI	CTCTTC	4 cut(s)	507, 798, 945, 1225
Eco130I	CCWWGG	1 cut(s)	1241
Eco32I	GATATC	1 cut(s)	468
Eco47III	AGCGCT	1 cut(s)	809
Eco57I	CTGAAG	4 cut(s)	822, 961, 1115, 1351
EcoRI	GAATTC	1 cut(s)	1079
EcoRII	CCWGG	1 cut(s)	1250
EcoRV	GATATC	1 cut(s)	468
EcoT14I	CCWWGG	1 cut(s)	1241
ErhI	CCWWGG	1 cut(s)	1241
FaeI	CATG	5 cut(s)	50, 98, 1112, 1217, 1245
FalI	AAGNNNNNCTT	2 cut(s)	1068, 1100
FaqI	GGGAC	2 cut(s)	300, 652
FatI	CATG	5 cut(s)	46, 94, 1108, 1213, 1241
FauNDI	CATATG	1 cut(s)	946
FblI	GTMKAC	1 cut(s)	1317
Fnu4HI	GCNGC	4 cut(s)	100, 534, 720, 1044
FokI	GGATG	4 cut(s)	143, 492, 1069, 1267
Fsp4HI	GCNGC	4 cut(s)	100, 534, 720, 1044
FspBI	CTAG	5 cut(s)	725, 884, 1169, 1229, 1286
GlaI	GCGC	3 cut(s)	809, 1133, 1372
GluI	GCNGC	4 cut(s)	100, 534, 720, 1044
GsuI	CTGGAG	2 cut(s)	584, 726
HaeII	RGCGCY	1 cut(s)	811
HaeIII	GGCC	2 cut(s)	906, 1233
HapII	CCGG	3 cut(s)	510, 620, 902
HhaI	GCGC	3 cut(s)	810, 1134, 1373
Hin1II	CATG	5 cut(s)	50, 98, 1112, 1217, 1245
Hin6I	GCGC	3 cut(s)	808, 1132, 1371
HinP1I	GCGC	3 cut(s)	808, 1132, 1371
HinfI	GANTC	3 cut(s)	240, 1157, 1271
HpaII	CCGG	3 cut(s)	510, 620, 902
HphI	GGTGA	4 cut(s)	289, 298, 467, 524
Hpy166II	GTNNAC	5 cut(s)	297, 388, 442, 925, 1318
Hpy188I	TCNGA	6 cut(s)	217, 408, 802, 859, 871, 1031
Hpy188III	TCNNGA	6 cut(s)	208, 490, 660, 678, 846, 1328
Hpy8I	GTNNAC	5 cut(s)	297, 388, 442, 925, 1318
HpyAV	CCTTC	4 cut(s)	8, 52, 760, 1090
HpyCH4III	ACNGT	5 cut(s)	439, 798, 877, 922, 1309
HpyCH4IV	ACGT	2 cut(s)	293, 432
HpyCH4V	TGCA	8 cut(s)	11, 90, 379, 1022, 1066, 1213, 1258, 1277
HpyF10VI	GCNNNNNNNGC	5 cut(s)	96, 344, 710, 719, 725
HpyF3I	CTNAG	6 cut(s)	84, 109, 405, 607, 858, 1267
HpySE526I	ACGT	2 cut(s)	293, 432
Hsp92II	CATG	5 cut(s)	50, 98, 1112, 1217, 1245
HspAI	GCGC	3 cut(s)	808, 1132, 1371
KroI	GCCGGC	1 cut(s)	619
KroNI	GCCGGC	1 cut(s)	621
Kzo9I	GATC	2 cut(s)	342, 628
LguI	GCTCTTC	3 cut(s)	798, 945, 1225
LmnI	GCTCC	4 cut(s)	427, 683, 710, 1007
Lsp1109I	GCAGC	4 cut(s)	86, 520, 706, 1055
LweI	GCATC	1 cut(s)	1245
MaeI	CTAG	5 cut(s)	725, 884, 1169, 1229, 1286
MaeII	ACGT	2 cut(s)	293, 432
MaeIII	GTNAC	4 cut(s)	260, 433, 1193, 1328
MalI	GATC	2 cut(s)	344, 630
MboI	GATC	2 cut(s)	342, 628
MluCI	AATT	9 cut(s)	32, 183, 426, 735, 819, 849, 987, 1079, 1415
MlyI	GAGTC	1 cut(s)	1151
MmeI	TCCRAC	1 cut(s)	609
MroNI	GCCGGC	1 cut(s)	619
MroXI	GAANNNNTTC	2 cut(s)	853, 1419
MseI	TTAA	5 cut(s)	429, 839, 1121, 1199, 1352
MslI	CAYNNNNRTG	4 cut(s)	510, 831, 1212, 1240
MspA1I	CMGCKG	1 cut(s)	668
MspCI	CTTAAG	1 cut(s)	1198
MspI	CCGG	3 cut(s)	510, 620, 902
MspR9I	CCNGG	3 cut(s)	510, 903, 1252
MvaI	CCWGG	1 cut(s)	1252
MwoI	GCNNNNNNNGC	5 cut(s)	96, 344, 710, 719, 725
NaeI	GCCGGC	1 cut(s)	621
NciI	CCSGG	2 cut(s)	510, 903
NcoI	CCATGG	1 cut(s)	1241
NdeI	CATATG	1 cut(s)	946
NdeII	GATC	2 cut(s)	342, 628
NgoMIV	GCCGGC	1 cut(s)	619
NlaIII	CATG	5 cut(s)	50, 98, 1112, 1217, 1245
NlaIV	GGNNCC	1 cut(s)	780
NmuCI	GTSAC	2 cut(s)	260, 1328
NspI	RCATGY	1 cut(s)	1112
PciI	ACATGT	1 cut(s)	1108
PciSI	GCTCTTC	3 cut(s)	798, 945, 1225
PdiI	GCCGGC	1 cut(s)	621
PdmI	GAANNNNTTC	2 cut(s)	853, 1419
PfeI	GAWTC	2 cut(s)	240, 1271
PflFI	GACNNNGTC	1 cut(s)	608
PkrI	GCNGC	4 cut(s)	101, 535, 721, 1045
PleI	GAGTC	1 cut(s)	1151
PpsI	GAGTC	1 cut(s)	1151
PscI	ACATGT	1 cut(s)	1108
Psp6I	CCWGG	1 cut(s)	1250
PspGI	CCWGG	1 cut(s)	1250
PspN4I	GGNNCC	1 cut(s)	780
PspPI	GGNCC	2 cut(s)	904, 1232
PsyI	GACNNNGTC	1 cut(s)	608
PvuII	CAGCTG	1 cut(s)	668
RsaI	GTAC	4 cut(s)	194, 235, 572, 1181
RsaNI	GTAC	4 cut(s)	193, 234, 571, 1180
RseI	CAYNNNNRTG	4 cut(s)	510, 831, 1212, 1240
SapI	GCTCTTC	3 cut(s)	798, 945, 1225
SaqAI	TTAA	5 cut(s)	429, 839, 1121, 1199, 1352
SatI	GCNGC	4 cut(s)	100, 534, 720, 1044
Sau3AI	GATC	2 cut(s)	342, 628
Sau96I	GGNCC	2 cut(s)	904, 1232
SchI	GAGTC	1 cut(s)	1151
ScrFI	CCNGG	3 cut(s)	510, 903, 1252
SfaNI	GCATC	1 cut(s)	1245
SfcI	CTRYAG	1 cut(s)	363
SmiMI	CAYNNNNRTG	4 cut(s)	510, 831, 1212, 1240
SmlI	CTYRAG	4 cut(s)	188, 201, 676, 1198
SmoI	CTYRAG	4 cut(s)	188, 201, 676, 1198
Sse9I	AATT	9 cut(s)	32, 183, 426, 735, 819, 849, 987, 1079, 1415
SsiI	CCGC	3 cut(s)	453, 536, 891
SspMI	CTAG	5 cut(s)	725, 884, 1169, 1229, 1286
StyD4I	CCNGG	3 cut(s)	508, 901, 1250
StyI	CCWWGG	1 cut(s)	1241
TaaI	ACNGT	5 cut(s)	439, 798, 877, 922, 1309
TaiI	ACGT	2 cut(s)	296, 435
TaqI	TCGA	2 cut(s)	238, 1152
TasI	AATT	9 cut(s)	32, 183, 426, 735, 819, 849, 987, 1079, 1415
TatI	WGTACW	3 cut(s)	192, 570, 1179
TfiI	GAWTC	2 cut(s)	240, 1271
Tru1I	TTAA	5 cut(s)	429, 839, 1121, 1199, 1352
Tru9I	TTAA	5 cut(s)	429, 839, 1121, 1199, 1352
TscAI	CASTG	3 cut(s)	444, 939, 1312
TseFI	GTSAC	2 cut(s)	260, 1328
TseI	GCWGC	4 cut(s)	99, 533, 719, 1043
Tsp45I	GTSAC	2 cut(s)	260, 1328
TspDTI	ATGAA	4 cut(s)	494, 963, 1103, 1313
TspRI	CASTG	3 cut(s)	444, 939, 1312
Tth111I	GACNNNGTC	1 cut(s)	608
Vha464I	CTTAAG	1 cut(s)	1198
XapI	RAATTY	4 cut(s)	183, 819, 1079, 1415
XceI	RCATGY	1 cut(s)	1112
XmiI	GTMKAC	1 cut(s)	1317
XmnI	GAANNNNTTC	2 cut(s)	853, 1419
XspI	CTAG	5 cut(s)	725, 884, 1169, 1229, 1286

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.