RLG00000025700

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001499
Physical Location & Seq
Forward (+)
47724320 .. 47726818
2499 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025700

Sequence Viewer

Length: 549 bp
ATGGATTCGACAGCTTCACCGCCTCCCTATTATCACCATTCTCGTTCGGAGTCTTCGTTCCTAACCAAATCGGCGTCGCGACTTGCAAATCTTCAGCTTCGCTCCCTGGGTCTCATTCAATCACCTTCACCTTCATCTCGCAAATCTTCAGCTTGGCTCCCTTTGCACGAGCTACAGCTACTCTCGCCTTCGCCCAATCCAAGAACGTCCAAGACCCGTCTCGTCGATATGTTTGAAATGCCCGATGAGTTTGGTCCCTTATGTGCCCTCCCCAACTTCATCTACAAACTATTGTATCCAACATCACTGAGCTTCATGCAAAGTTCACGTTATGCAAATGAAAGTTCTATATTAGCCGTCACTGAAGGTTGCCAGGTCAAGAATCCTACAGCTCGCATTGAACACGAAGCCAGTACCTCCAAAATTGTTGAAGACCAGCAATTTTACTTTCAGCAAAGGGGAATAGACTATGAGAAAGCCATGGCTGCCATGATATCTCGTTTTTTCCAGGCAGTATTCAATGAGCTTCCTGACGAGTTTGGATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Reticulon

Protein Analysis

183

Amino Acids

20.52

Weight (kDa)

6.42

Isoelectric Point (pI)

67.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SUFBD_core PF01458 125 - 167 1e-08 SUF system FeS cluster assembly, SufBD core domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 79
AciI CCGC 1 cut(s) 20
AcuI CTGAAG 3 cut(s) 77, 132, 384
AcyI GRCGYC 1 cut(s) 74
AfaI GTAC 1 cut(s) 415
AgsI TTSAA 5 cut(s) 119, 236, 401, 431, 520
AjnI CCWGG 3 cut(s) 105, 372, 507
AloI GAACNNNNNNTCC 2 cut(s) 41, 73
AluBI AGCT 8 cut(s) 14, 97, 152, 172, 178, 312, 392, 526
AluI AGCT 8 cut(s) 14, 97, 152, 172, 178, 312, 392, 526
Alw26I GTCTC 2 cut(s) 116, 224
ApeKI GCWGC 1 cut(s) 485
AspS9I GGNCC 1 cut(s) 254
AsuHPI GGTGA 4 cut(s) 9, 26, 114, 120
AvaII GGWCC 1 cut(s) 254
BaeGI GKGCMC 1 cut(s) 268
BaeI ACNNNNGTAYC 2 cut(s) 278, 311
BauI CACGAG 1 cut(s) 167
BbsI GAAGAC 2 cut(s) 45, 438
BbvI GCAGC 1 cut(s) 472
BceAI ACGGC 1 cut(s) 341
BciT130I CCWGG 3 cut(s) 107, 374, 509
BciVI GTATCC 1 cut(s) 306
BcoDI GTCTC 2 cut(s) 116, 224
BfmI CTRYAG 2 cut(s) 173, 387
BfuI GTATCC 1 cut(s) 306
BisI GCNGC 1 cut(s) 486
BlsI GCNGC 1 cut(s) 487
Bme1390I CCNGG 3 cut(s) 107, 374, 509
Bme18I GGWCC 1 cut(s) 254
BmgT120I GGNCC 1 cut(s) 254
BmiI GGNNCC 2 cut(s) 158, 256
BmrFI CCNGG 3 cut(s) 107, 374, 509
BpiI GAAGAC 2 cut(s) 45, 438
BsaHI GRCGYC 1 cut(s) 74
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 3 cut(s) 105, 106, 480
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bse1I ACTGG 1 cut(s) 411
BseBI CCWGG 3 cut(s) 107, 374, 509
BseDI CCNNGG 3 cut(s) 105, 106, 480
BseMII CTCAG 1 cut(s) 299
BseNI ACTGG 1 cut(s) 411
BseSI GKGCMC 1 cut(s) 268
BseXI GCAGC 1 cut(s) 472
Bsh1236I CGCG 1 cut(s) 79
BslFI GGGAC 1 cut(s) 240
BsmAI GTCTC 2 cut(s) 116, 224
BsmBI CGTCTC 1 cut(s) 224
BsmFI GGGAC 1 cut(s) 240
Bso31I GGTCTC 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 268
Bsp143I GATC 1 cut(s) 542
Bsp19I CCATGG 1 cut(s) 480
Bsp68I TCGCGA 1 cut(s) 79
BspACI CCGC 1 cut(s) 20
BspCNI CTCAG 1 cut(s) 300
BspFNI CGCG 1 cut(s) 79
BspLI GGNNCC 2 cut(s) 158, 256
BspTNI GGTCTC 1 cut(s) 116
BsrI ACTGG 1 cut(s) 411
BssECI CCNNGG 3 cut(s) 105, 106, 480
BssMI GATC 1 cut(s) 542
BssNI GRCGYC 1 cut(s) 74
BssSI CACGAG 1 cut(s) 167
BssT1I CCWWGG 1 cut(s) 480
Bst2BI CACGAG 1 cut(s) 167
Bst2UI CCWGG 3 cut(s) 107, 374, 509
BstACI GRCGYC 1 cut(s) 74
BstC8I GCNNGC 1 cut(s) 394
BstDEI CTNAG 2 cut(s) 308, 546
BstDSI CCRYGG 1 cut(s) 480
BstFNI CGCG 1 cut(s) 79
BstKTI GATC 1 cut(s) 545
BstMAI GTCTC 2 cut(s) 116, 224
BstMBI GATC 1 cut(s) 542
BstMWI GCNNNNNNNGC 3 cut(s) 163, 184, 485
BstNI CCWGG 3 cut(s) 107, 374, 509
BstSCI CCNGG 3 cut(s) 105, 372, 507
BstSFI CTRYAG 2 cut(s) 173, 387
BstSLI GKGCMC 1 cut(s) 268
BstUI CGCG 1 cut(s) 79
BstV1I GCAGC 1 cut(s) 472
BstV2I GAAGAC 2 cut(s) 45, 438
BstX2I RGATCY 1 cut(s) 542
BstYI RGATCY 1 cut(s) 542
BsuI GTATCC 1 cut(s) 306
BtgI CCRYGG 1 cut(s) 480
BtsIMutI CAGTG 2 cut(s) 305, 360
BtuMI TCGCGA 1 cut(s) 79
Cac8I GCNNGC 1 cut(s) 394
Cfr13I GGNCC 1 cut(s) 254
CseI GACGC 1 cut(s) 63
Csp6I GTAC 1 cut(s) 414
CviAII CATG 3 cut(s) 316, 481, 490
CviQI GTAC 1 cut(s) 414
DdeI CTNAG 2 cut(s) 308, 546
DpnI GATC 1 cut(s) 544
DpnII GATC 1 cut(s) 542
Eco130I CCWWGG 1 cut(s) 480
Eco31I GGTCTC 1 cut(s) 116
Eco32I GATATC 1 cut(s) 495
Eco47I GGWCC 1 cut(s) 254
Eco57I CTGAAG 3 cut(s) 77, 132, 384
EcoRII CCWGG 3 cut(s) 105, 372, 507
EcoRV GATATC 1 cut(s) 495
EcoT14I CCWWGG 1 cut(s) 480
ErhI CCWWGG 1 cut(s) 480
Esp3I CGTCTC 1 cut(s) 224
FaeI CATG 3 cut(s) 319, 484, 493
FaiI YATR 8 cut(s) 230, 262, 317, 333, 350, 471, 482, 491
FaqI GGGAC 1 cut(s) 240
FatI CATG 3 cut(s) 315, 480, 489
Fnu4HI GCNGC 1 cut(s) 486
Fsp4HI GCNGC 1 cut(s) 486
GluI GCNGC 1 cut(s) 486
HgaI GACGC 1 cut(s) 63
Hin1I GRCGYC 1 cut(s) 74
Hin1II CATG 3 cut(s) 319, 484, 493
HinfI GANTC 3 cut(s) 5, 50, 382
HphI GGTGA 4 cut(s) 9, 26, 114, 120
Hpy166II GTNNAC 1 cut(s) 326
Hpy188I TCNGA 1 cut(s) 49
Hpy188III TCNNGA 3 cut(s) 78, 379, 530
Hpy8I GTNNAC 1 cut(s) 326
Hpy99I CGWCG 2 cut(s) 79, 227
HpyAV CCTTC 4 cut(s) 135, 141, 198, 359
HpyCH4IV ACGT 2 cut(s) 206, 328
HpyCH4V TGCA 4 cut(s) 86, 166, 319, 335
HpyF10VI GCNNNNNNNGC 3 cut(s) 163, 184, 485
HpyF3I CTNAG 2 cut(s) 308, 546
HpySE526I ACGT 2 cut(s) 206, 328
Hsp92I GRCGYC 1 cut(s) 74
Hsp92II CATG 3 cut(s) 319, 484, 493
Kzo9I GATC 1 cut(s) 542
LmnI GCTCC 2 cut(s) 107, 162
LpnPI CCDG 9 cut(s) 92, 119, 359, 386, 424, 449, 494, 521, 543
Lsp1109I GCAGC 1 cut(s) 472
MaeII ACGT 2 cut(s) 206, 328
MaeIII GTNAC 1 cut(s) 358
MalI GATC 1 cut(s) 544
MboI GATC 1 cut(s) 542
MboII GAAGA 4 cut(s) 45, 83, 138, 443
MflI RGATCY 1 cut(s) 542
MhlI GDGCHC 1 cut(s) 268
MluCI AATT 2 cut(s) 423, 440
MlyI GAGTC 1 cut(s) 59
MmeI TCCRAC 1 cut(s) 323
MnlI CCTC 3 cut(s) 33, 278, 427
MspR9I CCNGG 3 cut(s) 107, 374, 509
MvaI CCWGG 3 cut(s) 107, 374, 509
MvnI CGCG 1 cut(s) 79
MwoI GCNNNNNNNGC 3 cut(s) 163, 184, 485
NcoI CCATGG 1 cut(s) 480
NdeII GATC 1 cut(s) 542
NlaIII CATG 3 cut(s) 319, 484, 493
NlaIV GGNNCC 2 cut(s) 158, 256
NmuCI GTSAC 1 cut(s) 358
NruI TCGCGA 1 cut(s) 79
PasI CCCWGGG 1 cut(s) 106
PcsI WCGNNNNNNNCGW 1 cut(s) 53
PfeI GAWTC 2 cut(s) 5, 382
PkrI GCNGC 1 cut(s) 487
PleI GAGTC 1 cut(s) 58
PpsI GAGTC 1 cut(s) 58
Psp6I CCWGG 3 cut(s) 105, 372, 507
PspGI CCWGG 3 cut(s) 105, 372, 507
PspN4I GGNNCC 2 cut(s) 158, 256
PspPI GGNCC 1 cut(s) 254
PsuI RGATCY 1 cut(s) 542
RruI TCGCGA 1 cut(s) 79
RsaI GTAC 1 cut(s) 415
RsaNI GTAC 1 cut(s) 414
SatI GCNGC 1 cut(s) 486
Sau3AI GATC 1 cut(s) 542
Sau96I GGNCC 1 cut(s) 254
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 3 cut(s) 107, 374, 509
SduI GDGCHC 1 cut(s) 268
SfcI CTRYAG 2 cut(s) 173, 387
SinI GGWCC 1 cut(s) 254
Sse9I AATT 2 cut(s) 423, 440
SsiI CCGC 1 cut(s) 20
StyD4I CCNGG 3 cut(s) 105, 372, 507
StyI CCWWGG 1 cut(s) 480
TaiI ACGT 2 cut(s) 209, 331
TaqI TCGA 2 cut(s) 8, 225
TasI AATT 2 cut(s) 423, 440
TfiI GAWTC 2 cut(s) 5, 382
TscAI CASTG 2 cut(s) 312, 367
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 1 cut(s) 485
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 4 cut(s) 123, 268, 304, 354
TspRI CASTG 2 cut(s) 312, 367
VpaK11BI GGWCC 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.