Rmu_sc0000538.1_g000047

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000538.1
OG0001499
Physical Location & Seq
Reverse (-)
168406 .. 170489
2084 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000538.1_g000047.1.cds

Sequence Viewer

Length: 591 bp
atgcttccttatgcatttgttatctcgtttgttgttatcttgtttgctgctattcttgaaaacaaaactgctattactgggggtagtggcaaagttgtggacagtggtccgaatgatcacatcatcatatactactccgatcatgctggtcctggagtacttgggatgcctaccagtccctatatttatgcaaacagactcatagaagttctaaagaagaagcatgctgcagggacatatgaaagtttggtattttatcttgaagcttgtgaatccggaagtatctttgagggtcttcttcctgaaggtctgttcaagaaatatttagattcccttactttggatgtcttccggttggtctctgatggcaattatatgatctcattatgctggcccaggtcaaatcaaggacagcgagtcaagaatcctatagctcgcattgaacacgaagccaatacctccaaaattggtgaagaccatcaattttactttcagcaaaggggaatagactatgagaaagccatggctgccatgatatcttgtttttgccagacagtattcaatgagcttcctgacgagtttgggtcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

196

Amino Acids

21.85

Weight (kDa)

5.34

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 275
AcuI CTGAAG 1 cut(s) 324
AfaI GTAC 1 cut(s) 159
AfiI CCNNNNNNNGG 1 cut(s) 340
AgsI TTSAA 5 cut(s) 59, 263, 316, 443, 562
AhdI GACNNNNNGTC 1 cut(s) 416
AjnI CCWGG 2 cut(s) 151, 395
AluBI AGCT 3 cut(s) 266, 434, 568
AluI AGCT 3 cut(s) 266, 434, 568
Alw26I GTCTC 1 cut(s) 364
Aor13HI TCCGGA 1 cut(s) 275
AoxI GGCC 1 cut(s) 392
ApeKI GCWGC 3 cut(s) 47, 227, 527
AspS9I GGNCC 3 cut(s) 107, 149, 393
AsuHPI GGTGA 1 cut(s) 482
AvaII GGWCC 2 cut(s) 107, 149
BbsI GAAGAC 3 cut(s) 287, 340, 480
BbvI GCAGC 3 cut(s) 34, 214, 514
BccI CCATC 2 cut(s) 359, 486
BciT130I CCWGG 2 cut(s) 153, 397
BclI TGATCA 1 cut(s) 115
BcoDI GTCTC 1 cut(s) 364
BfmI CTRYAG 2 cut(s) 228, 429
BisI GCNGC 3 cut(s) 48, 228, 528
BlsI GCNGC 3 cut(s) 49, 229, 529
BmcAI AGTACT 1 cut(s) 159
Bme1390I CCNGG 2 cut(s) 153, 397
Bme18I GGWCC 2 cut(s) 107, 149
BmeRI GACNNNNNGTC 1 cut(s) 416
BmgT120I GGNCC 3 cut(s) 107, 149, 393
BmrFI CCNGG 2 cut(s) 153, 397
BmrI ACTGGG 1 cut(s) 87
BmsI GCATC 1 cut(s) 156
BmuI ACTGGG 1 cut(s) 87
BoxI GACNNNNGTC 1 cut(s) 105
BpiI GAAGAC 3 cut(s) 287, 340, 480
BpmI CTGGAG 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 364
BsaJI CCNNGG 2 cut(s) 395, 522
BsaWI WCCGGW 2 cut(s) 275, 351
Bsc4I CCNNNNNNNGG 1 cut(s) 340
Bse1I ACTGG 2 cut(s) 82, 174
BseAI TCCGGA 1 cut(s) 275
BseBI CCWGG 2 cut(s) 153, 397
BseDI CCNNGG 2 cut(s) 395, 522
BseGI GGATG 2 cut(s) 171, 349
BseLI CCNNNNNNNGG 1 cut(s) 340
BseNI ACTGG 2 cut(s) 82, 174
BseXI GCAGC 3 cut(s) 34, 214, 514
BshFI GGCC 1 cut(s) 394
BsiSI CCGG 2 cut(s) 276, 352
BslFI GGGAC 2 cut(s) 162, 247
BslI CCNNNNNNNGG 1 cut(s) 340
BsmAI GTCTC 1 cut(s) 364
BsmFI GGGAC 2 cut(s) 162, 247
BsnI GGCC 1 cut(s) 394
Bso31I GGTCTC 1 cut(s) 364
Bsp13I TCCGGA 1 cut(s) 275
Bsp143I GATC 3 cut(s) 115, 139, 378
Bsp19I CCATGG 1 cut(s) 522
BspANI GGCC 1 cut(s) 394
BspEI TCCGGA 1 cut(s) 275
BspMAI CTGCAG 1 cut(s) 232
BspTNI GGTCTC 1 cut(s) 364
BsrI ACTGG 2 cut(s) 82, 174
BssECI CCNNGG 2 cut(s) 395, 522
BssMI GATC 3 cut(s) 115, 139, 378
BssT1I CCWWGG 1 cut(s) 522
Bst2UI CCWGG 2 cut(s) 153, 397
Bst4CI ACNGT 2 cut(s) 104, 556
BstC8I GCNNGC 3 cut(s) 225, 392, 436
BstDEI CTNAG 1 cut(s) 588
BstDSI CCRYGG 1 cut(s) 522
BstF5I GGATG 2 cut(s) 171, 349
BstKTI GATC 3 cut(s) 118, 142, 381
BstMAI GTCTC 1 cut(s) 364
BstMBI GATC 3 cut(s) 115, 139, 378
BstMWI GCNNNNNNNGC 1 cut(s) 527
BstNI CCWGG 2 cut(s) 153, 397
BstNSI RCATGY 1 cut(s) 227
BstPAI GACNNNNGTC 1 cut(s) 105
BstSCI CCNGG 2 cut(s) 151, 395
BstSFI CTRYAG 2 cut(s) 228, 429
BstV1I GCAGC 3 cut(s) 34, 214, 514
BstV2I GAAGAC 3 cut(s) 287, 340, 480
BsuRI GGCC 1 cut(s) 394
BtgI CCRYGG 1 cut(s) 522
BtsCI GGATG 2 cut(s) 171, 349
BtsIMutI CAGTG 1 cut(s) 109
Cac8I GCNNGC 3 cut(s) 225, 392, 436
Cfr13I GGNCC 3 cut(s) 107, 149, 393
Csp6I GTAC 1 cut(s) 158
CviAII CATG 4 cut(s) 143, 224, 523, 532
CviJI RGCY 7 cut(s) 266, 394, 434, 452, 521, 527, 568
CviKI_1 RGCY 7 cut(s) 266, 394, 434, 452, 521, 527, 568
CviQI GTAC 1 cut(s) 158
DdeI CTNAG 1 cut(s) 588
DpnI GATC 3 cut(s) 117, 141, 380
DpnII GATC 3 cut(s) 115, 139, 378
DriI GACNNNNNGTC 1 cut(s) 416
Eam1105I GACNNNNNGTC 1 cut(s) 416
Eco130I CCWWGG 1 cut(s) 522
Eco31I GGTCTC 1 cut(s) 364
Eco32I GATATC 1 cut(s) 537
Eco47I GGWCC 2 cut(s) 107, 149
Eco57I CTGAAG 1 cut(s) 324
EcoRII CCWGG 2 cut(s) 151, 395
EcoRV GATATC 1 cut(s) 537
EcoT14I CCWWGG 1 cut(s) 522
EcoT22I ATGCAT 1 cut(s) 16
ErhI CCWWGG 1 cut(s) 522
FaeI CATG 4 cut(s) 146, 227, 526, 535
FaqI GGGAC 2 cut(s) 162, 247
FatI CATG 4 cut(s) 142, 223, 522, 531
FauNDI CATATG 1 cut(s) 238
FbaI TGATCA 1 cut(s) 115
Fnu4HI GCNGC 3 cut(s) 48, 228, 528
FokI GGATG 2 cut(s) 178, 356
Fsp4HI GCNGC 3 cut(s) 48, 228, 528
GluI GCNGC 3 cut(s) 48, 228, 528
GsuI CTGGAG 1 cut(s) 174
HaeIII GGCC 1 cut(s) 394
HapII CCGG 2 cut(s) 276, 352
Hin1II CATG 4 cut(s) 146, 227, 526, 535
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 5 cut(s) 198, 272, 329, 417, 424
HpaII CCGG 2 cut(s) 276, 352
HphI GGTGA 1 cut(s) 482
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 3 cut(s) 111, 139, 364
Hpy188III TCNNGA 7 cut(s) 56, 260, 276, 302, 316, 421, 572
Hpy8I GTNNAC 1 cut(s) 100
HpyAV CCTTC 1 cut(s) 299
HpyCH4III ACNGT 2 cut(s) 104, 556
HpyCH4V TGCA 3 cut(s) 14, 191, 230
HpyF10VI GCNNNNNNNGC 1 cut(s) 527
HpyF3I CTNAG 1 cut(s) 588
Hsp92II CATG 4 cut(s) 146, 227, 526, 535
Kpn2I TCCGGA 1 cut(s) 275
Ksp22I TGATCA 1 cut(s) 115
Kzo9I GATC 3 cut(s) 115, 139, 378
Lsp1109I GCAGC 3 cut(s) 34, 214, 514
LweI GCATC 1 cut(s) 156
MalI GATC 3 cut(s) 117, 141, 380
MboI GATC 3 cut(s) 115, 139, 378
MboII GAAGA 5 cut(s) 229, 287, 290, 340, 485
MluCI AATT 3 cut(s) 370, 465, 482
MlyI GAGTC 2 cut(s) 192, 426
MnlI CCTC 2 cut(s) 283, 469
Mph1103I ATGCAT 1 cut(s) 16
MroI TCCGGA 1 cut(s) 275
MspI CCGG 2 cut(s) 276, 352
MspR9I CCNGG 2 cut(s) 153, 397
MvaI CCWGG 2 cut(s) 153, 397
MwoI GCNNNNNNNGC 1 cut(s) 527
NcoI CCATGG 1 cut(s) 522
NdeI CATATG 1 cut(s) 238
NdeII GATC 3 cut(s) 115, 139, 378
NlaIII CATG 4 cut(s) 146, 227, 526, 535
NsiI ATGCAT 1 cut(s) 16
NspI RCATGY 1 cut(s) 227
PaeI GCATGC 1 cut(s) 227
PfeI GAWTC 3 cut(s) 272, 329, 424
PfoI TCCNGGA 1 cut(s) 151
PkrI GCNGC 3 cut(s) 49, 229, 529
PleI GAGTC 2 cut(s) 192, 425
PpsI GAGTC 2 cut(s) 192, 425
PshAI GACNNNNGTC 1 cut(s) 105
Psp6I CCWGG 2 cut(s) 151, 395
PspGI CCWGG 2 cut(s) 151, 395
PspPI GGNCC 3 cut(s) 107, 149, 393
PstI CTGCAG 1 cut(s) 232
RsaI GTAC 1 cut(s) 159
RsaNI GTAC 1 cut(s) 158
SatI GCNGC 3 cut(s) 48, 228, 528
Sau3AI GATC 3 cut(s) 115, 139, 378
Sau96I GGNCC 3 cut(s) 107, 149, 393
ScaI AGTACT 1 cut(s) 159
SchI GAGTC 2 cut(s) 192, 426
ScrFI CCNGG 2 cut(s) 153, 397
SetI ASST 6 cut(s) 268, 310, 401, 436, 461, 570
SfaNI GCATC 1 cut(s) 156
SfcI CTRYAG 2 cut(s) 228, 429
SinI GGWCC 2 cut(s) 107, 149
SphI GCATGC 1 cut(s) 227
Sse9I AATT 3 cut(s) 370, 465, 482
SspI AATATT 1 cut(s) 323
StyD4I CCNGG 2 cut(s) 151, 395
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 2 cut(s) 104, 556
TasI AATT 3 cut(s) 370, 465, 482
TatI WGTACW 1 cut(s) 157
TfiI GAWTC 3 cut(s) 272, 329, 424
TscAI CASTG 1 cut(s) 109
TseI GCWGC 3 cut(s) 47, 227, 527
TspDTI ATGAA 1 cut(s) 255
TspRI CASTG 1 cut(s) 109
VpaK11BI GGWCC 2 cut(s) 107, 149
XceI RCATGY 1 cut(s) 227
ZrmI AGTACT 1 cut(s) 159
Zsp2I ATGCAT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.