Rmu_sc0000322.1_g000042

CCR4-NOT transcription complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000322.1
OG0003483
Physical Location & Seq
Reverse (-)
173534 .. 179174
5641 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1341 bp
atggagatccgcatctccctaggattttccggcttcgtggcccataacctagcctcctctgccaatattcacgtcaataattgctgtggcatccacaggtgctgggttaggtctctgctattcggcactaaccaggcggtagcaacaattgacggggaaataggtccacagctttcagtaagaagaaagagagaaggtgttggtgcaacctattttgatgcaaacatgtatccacaaggttctatgggggttgtgccagaagctctccgccctaaacctggtcatttgtccctttctcagcaacgagtttatgaggtaagatgctaccttttacttgggagatcttctcctttgaagaatgccttgtgtactggtctcactagcacatatggttcagcatccggacaacttaacacgggctactcaccaggacctggaagcaaatttgaagatgtttctcgtccattagatgaagcgatggaatccaattctgccctccatcttagtgcttcatctattcatgttggggcggctgatggtgtttcccagcatagttctgaaaatgattctgttgttgcctcatttccttctgctgtttctgcccctgagctgcactctgtagagtcatctgatgctgtaaaggaatctggagtgtcctcacagccaccaccttcacctgctgtaactgaacgtcttggaagtctctcagaaccttccctcaccacaggggctgcattggaaaaataccagattgttgcacagaaggtcaacctttttagcttttacttatctgatgtttgtctagtcctatttgtattggggatgggttcttggtctatttataaaggttcgagaagtgttgtgtttgagaagcattctattgagaggtaccagagagaccagcggctatacaataaccaattggaggaaaccactctatgctctctccttcctgattgtgatgctgattctgatttcacaaggcaaaatgacattgcattgcagctaccagagctgagaaagctgcttcaggtgttgagagaggagagagagggaaggagggtgtggctgtatgataggttggtgtccaatgatgtgttggattttgttgcctctgatgctcgacctgtctatgtgggcaagactgctgggtaccatagcagaacccaggaggagatacatgaattgctactgagttttgctgaagctggagctactgttgtcagactaaaaggaggggatccattggtgtttagaaggggtggagaggagacggattttttgcaacagcaaggaattcaagtgcaagttattccaggtacaataatgactcagtactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CNOT1_CAF1_bind CNOT1_HEAT CNOT1_TTP_bind DUF3819 Not1

Protein Analysis

446

Amino Acids

48.71

Weight (kDa)

5.71

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP_methylase PF00590 354 - 440 1.1e-12 Tetrapyrrole (Corrin/Porphyrin) Methylases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 843
AarI CACCTGC 1 cut(s) 685
Acc36I ACCTGC 1 cut(s) 685
Acc65I GGTACC 2 cut(s) 888, 1152
AccB1I GGYRCC 2 cut(s) 888, 1152
AccB7I CCANNNNNTGG 1 cut(s) 434
AccIII TCCGGA 1 cut(s) 401
AciI CCGC 5 cut(s) 10, 137, 268, 530, 904
AclWI GGATC 2 cut(s) 1235, 1248
AcsI RAATTY 2 cut(s) 443, 1296
AcuI CTGAAG 2 cut(s) 1013, 1224
AfaI GTAC 5 cut(s) 370, 890, 1154, 1321, 1337
AfiI CCNNNNNNNGG 3 cut(s) 278, 434, 925
AflIII ACRYGT 1 cut(s) 225
AgsI TTSAA 3 cut(s) 355, 449, 1301
AjiI CACGTC 1 cut(s) 73
AjnI CCWGG 6 cut(s) 132, 277, 427, 433, 1167, 1315
AluBI AGCT 9 cut(s) 172, 263, 610, 780, 1006, 1015, 1024, 1208, 1214
AluI AGCT 9 cut(s) 172, 263, 610, 780, 1006, 1015, 1024, 1208, 1214
Alw26I GTCTC 5 cut(s) 117, 380, 707, 891, 1265
AlwI GGATC 2 cut(s) 1235, 1248
AlwNI CAGNNNCTG 3 cut(s) 102, 434, 731
Aor13HI TCCGGA 1 cut(s) 401
AoxI GGCC 1 cut(s) 39
ApeKI GCWGC 4 cut(s) 610, 731, 1003, 1024
ApoI RAATTY 2 cut(s) 443, 1296
ArsI GACNNNNNNTTYG 2 cut(s) 1264, 1296
Asp718I GGTACC 2 cut(s) 888, 1152
AspA2I CCTAGG 1 cut(s) 19
AspS9I GGNCC 3 cut(s) 40, 164, 431
AsuHPI GGTGA 3 cut(s) 417, 666, 712
AvaII GGWCC 2 cut(s) 164, 431
AvrII CCTAGG 1 cut(s) 19
BamHI GGATCC 1 cut(s) 1240
BanI GGYRCC 2 cut(s) 888, 1152
BbvI GCAGC 4 cut(s) 597, 718, 1011, 1015
BccI CCATC 4 cut(s) 472, 507, 530, 817
BciT130I CCWGG 6 cut(s) 134, 279, 429, 435, 1169, 1317
BciVI GTATCC 1 cut(s) 240
BcoDI GTCTC 5 cut(s) 117, 380, 707, 891, 1265
BfaI CTAG 5 cut(s) 20, 50, 381, 803, 1339
BfmI CTRYAG 1 cut(s) 618
BfuAI ACCTGC 1 cut(s) 685
BfuI GTATCC 1 cut(s) 240
BglII AGATCT 1 cut(s) 341
BisI GCNGC 6 cut(s) 531, 611, 732, 905, 1004, 1025
BlnI CCTAGG 1 cut(s) 19
BlsI GCNGC 6 cut(s) 532, 612, 733, 906, 1005, 1026
BmcAI AGTACT 1 cut(s) 1337
Bme1390I CCNGG 6 cut(s) 134, 279, 429, 435, 1169, 1317
Bme18I GGWCC 2 cut(s) 164, 431
BmgBI CACGTC 1 cut(s) 73
BmgT120I GGNCC 3 cut(s) 40, 164, 431
BmiI GGNNCC 3 cut(s) 890, 1154, 1242
BmrFI CCNGG 6 cut(s) 134, 279, 429, 435, 1169, 1317
BmsI GCATC 8 cut(s) 21, 99, 208, 311, 407, 622, 952, 1108
BplI GAGNNNNNCTC 4 cut(s) 331, 363, 599, 631
BpmI CTGGAG 2 cut(s) 669, 1230
Bpu10I CCTNAGC 1 cut(s) 606
BsaBI GATNNNNATC 1 cut(s) 11
BsaI GGTCTC 3 cut(s) 117, 380, 891
BsaJI CCNNGG 2 cut(s) 19, 1167
BsaWI WCCGGW 1 cut(s) 401
BsaXI ACNNNNNCTCC 4 cut(s) 38, 68, 1203, 1233
Bsc4I CCNNNNNNNGG 3 cut(s) 278, 434, 925
Bse1I ACTGG 1 cut(s) 376
Bse3DI GCAATG 2 cut(s) 993, 998
Bse8I GATNNNNATC 1 cut(s) 11
BseAI TCCGGA 1 cut(s) 401
BseBI CCWGG 6 cut(s) 134, 279, 429, 435, 1169, 1317
BseDI CCNNGG 2 cut(s) 19, 1167
BseGI GGATG 3 cut(s) 90, 398, 828
BseJI GATNNNNATC 1 cut(s) 11
BseLI CCNNNNNNNGG 3 cut(s) 278, 434, 925
BseMI GCAATG 2 cut(s) 993, 998
BseMII CTCAG 5 cut(s) 311, 597, 720, 1007, 1184
BseNI ACTGG 1 cut(s) 376
BseRI GAGGAG 4 cut(s) 46, 1058, 1187, 1283
BseXI GCAGC 4 cut(s) 597, 718, 1011, 1015
BseYI CCCAGC 3 cut(s) 102, 546, 1148
BsgI GTGCAG 1 cut(s) 596
BshFI GGCC 1 cut(s) 41
BshNI GGYRCC 2 cut(s) 888, 1152
BsiSI CCGG 2 cut(s) 30, 402
BslFI GGGAC 1 cut(s) 274
BslI CCNNNNNNNGG 3 cut(s) 278, 434, 925
BsmAI GTCTC 5 cut(s) 117, 380, 707, 891, 1265
BsmBI CGTCTC 1 cut(s) 1265
BsmFI GGGAC 1 cut(s) 274
BsmI GAATGC 2 cut(s) 364, 874
BsnI GGCC 1 cut(s) 41
Bso31I GGTCTC 3 cut(s) 117, 380, 891
Bsp13I TCCGGA 1 cut(s) 401
Bsp143I GATC 3 cut(s) 6, 341, 1240
BspACI CCGC 5 cut(s) 10, 137, 268, 530, 904
BspANI GGCC 1 cut(s) 41
BspCNI CTCAG 5 cut(s) 310, 598, 719, 1008, 1185
BspEI TCCGGA 1 cut(s) 401
BspLI GGNNCC 3 cut(s) 890, 1154, 1242
BspMI ACCTGC 1 cut(s) 685
BspPI GGATC 2 cut(s) 1235, 1248
BspT107I GGYRCC 2 cut(s) 888, 1152
BspTNI GGTCTC 3 cut(s) 117, 380, 891
BsrDI GCAATG 2 cut(s) 993, 998
BsrI ACTGG 1 cut(s) 376
BssECI CCNNGG 2 cut(s) 19, 1167
BssMI GATC 3 cut(s) 6, 341, 1240
BssT1I CCWWGG 1 cut(s) 19
Bst2UI CCWGG 6 cut(s) 134, 279, 429, 435, 1169, 1317
Bst4CI ACNGT 1 cut(s) 1219
BstDEI CTNAG 7 cut(s) 297, 503, 606, 706, 1016, 1193, 1332
BstF5I GGATG 3 cut(s) 90, 398, 828
BstKTI GATC 3 cut(s) 9, 344, 1243
BstMAI GTCTC 5 cut(s) 117, 380, 707, 891, 1265
BstMBI GATC 3 cut(s) 6, 341, 1240
BstMWI GCNNNNNNNGC 5 cut(s) 59, 599, 1012, 1021, 1118
BstNI CCWGG 6 cut(s) 134, 279, 429, 435, 1169, 1317
BstNSI RCATGY 1 cut(s) 229
BstSCI CCNGG 6 cut(s) 132, 277, 427, 433, 1167, 1315
BstSFI CTRYAG 1 cut(s) 618
BstV1I GCAGC 4 cut(s) 597, 718, 1011, 1015
BstX2I RGATCY 3 cut(s) 6, 341, 1240
BstYI RGATCY 3 cut(s) 6, 341, 1240
BsuI GTATCC 1 cut(s) 240
BsuRI GGCC 1 cut(s) 41
BtgZI GCGATG 1 cut(s) 491
BtrI CACGTC 1 cut(s) 73
BtsCI GGATG 3 cut(s) 90, 398, 828
BveI ACCTGC 1 cut(s) 685
CaiI CAGNNNCTG 3 cut(s) 102, 434, 731
Cfr13I GGNCC 3 cut(s) 40, 164, 431
CsiI ACCWGGT 1 cut(s) 277
Csp6I GTAC 5 cut(s) 369, 889, 1153, 1320, 1336
CviAII CATG 3 cut(s) 226, 521, 1181
CviQI GTAC 5 cut(s) 369, 889, 1153, 1320, 1336
DdeI CTNAG 7 cut(s) 297, 503, 606, 706, 1016, 1193, 1332
DpnI GATC 3 cut(s) 8, 343, 1242
DpnII GATC 3 cut(s) 6, 341, 1240
EciI GGCGGA 1 cut(s) 257
Eco130I CCWWGG 1 cut(s) 19
Eco31I GGTCTC 3 cut(s) 117, 380, 891
Eco47I GGWCC 2 cut(s) 164, 431
Eco57I CTGAAG 2 cut(s) 1013, 1224
EcoO109I RGGNCCY 1 cut(s) 431
EcoRI GAATTC 1 cut(s) 1296
EcoRII CCWGG 6 cut(s) 132, 277, 427, 433, 1167, 1315
EcoT14I CCWWGG 1 cut(s) 19
ErhI CCWWGG 1 cut(s) 19
Esp3I CGTCTC 1 cut(s) 1265
FaeI CATG 3 cut(s) 229, 524, 1184
FalI AAGNNNNNCTT 2 cut(s) 347, 379
FaqI GGGAC 1 cut(s) 274
FatI CATG 3 cut(s) 225, 520, 1180
FauNDI CATATG 1 cut(s) 388
Fnu4HI GCNGC 6 cut(s) 531, 611, 732, 905, 1004, 1025
FokI GGATG 3 cut(s) 77, 385, 835
Fsp4HI GCNGC 6 cut(s) 531, 611, 732, 905, 1004, 1025
FspBI CTAG 5 cut(s) 20, 50, 381, 803, 1339
GluI GCNGC 6 cut(s) 531, 611, 732, 905, 1004, 1025
GsaI CCCAGC 3 cut(s) 106, 550, 1152
GsuI CTGGAG 2 cut(s) 669, 1230
HaeIII GGCC 1 cut(s) 41
HapII CCGG 2 cut(s) 30, 402
Hin1II CATG 3 cut(s) 229, 524, 1184
HincII GTYRAC 1 cut(s) 769
HindII GTYRAC 1 cut(s) 769
HinfI GANTC 6 cut(s) 482, 566, 623, 644, 968, 1330
HpaII CCGG 2 cut(s) 30, 402
HphI GGTGA 3 cut(s) 417, 666, 712
Hpy166II GTNNAC 3 cut(s) 167, 369, 769
Hpy188I TCNGA 7 cut(s) 559, 631, 709, 793, 973, 1117, 1226
Hpy188III TCNNGA 4 cut(s) 402, 648, 852, 953
Hpy8I GTNNAC 3 cut(s) 167, 369, 769
HpyAV CCTTC 8 cut(s) 188, 597, 681, 723, 757, 959, 1050, 1251
HpyCH4III ACNGT 1 cut(s) 1219
HpyCH4IV ACGT 2 cut(s) 72, 691
HpyCH4V TGCA 9 cut(s) 206, 221, 613, 734, 758, 998, 1003, 1285, 1306
HpyF10VI GCNNNNNNNGC 5 cut(s) 59, 599, 1012, 1021, 1118
HpyF3I CTNAG 7 cut(s) 297, 503, 606, 706, 1016, 1193, 1332
HpySE526I ACGT 2 cut(s) 72, 691
Hsp92II CATG 3 cut(s) 229, 524, 1184
Kpn2I TCCGGA 1 cut(s) 401
KpnI GGTACC 2 cut(s) 892, 1156
Kzo9I GATC 3 cut(s) 6, 341, 1240
LmnI GCTCC 1 cut(s) 1211
Lsp1109I GCAGC 4 cut(s) 597, 718, 1011, 1015
LweI GCATC 8 cut(s) 21, 99, 208, 311, 407, 622, 952, 1108
MabI ACCWGGT 1 cut(s) 277
MaeI CTAG 5 cut(s) 20, 50, 381, 803, 1339
MaeII ACGT 2 cut(s) 72, 691
MaeIII GTNAC 1 cut(s) 682
MalI GATC 3 cut(s) 8, 343, 1242
MboI GATC 3 cut(s) 6, 341, 1240
MboII GAAGA 4 cut(s) 195, 336, 367, 461
MfeI CAATTG 2 cut(s) 147, 920
MflI RGATCY 3 cut(s) 6, 341, 1240
MluCI AATT 7 cut(s) 79, 147, 443, 487, 920, 1184, 1296
MlyI GAGTC 2 cut(s) 632, 1324
MmeI TCCRAC 1 cut(s) 1080
MroI TCCGGA 1 cut(s) 401
MseI TTAA 1 cut(s) 411
MslI CAYNNNNRTG 1 cut(s) 504
MspA1I CMGCKG 1 cut(s) 904
MspI CCGG 2 cut(s) 30, 402
MspR9I CCNGG 6 cut(s) 134, 279, 429, 435, 1169, 1317
MunI CAATTG 2 cut(s) 147, 920
Mva1269I GAATGC 2 cut(s) 364, 874
MvaI CCWGG 6 cut(s) 134, 279, 429, 435, 1169, 1317
MwoI GCNNNNNNNGC 5 cut(s) 59, 599, 1012, 1021, 1118
NdeI CATATG 1 cut(s) 388
NdeII GATC 3 cut(s) 6, 341, 1240
NlaIII CATG 3 cut(s) 229, 524, 1184
NlaIV GGNNCC 3 cut(s) 890, 1154, 1242
NspI RCATGY 1 cut(s) 229
PaqCI CACCTGC 1 cut(s) 685
PciI ACATGT 1 cut(s) 225
PctI GAATGC 2 cut(s) 364, 874
PfeI GAWTC 4 cut(s) 482, 566, 644, 968
PflMI CCANNNNNTGG 1 cut(s) 434
PkrI GCNGC 6 cut(s) 532, 612, 733, 906, 1005, 1026
PleI GAGTC 2 cut(s) 631, 1324
PpsI GAGTC 2 cut(s) 631, 1324
PpuMI RGGWCCY 1 cut(s) 431
PscI ACATGT 1 cut(s) 225
PsiI TTATAA 1 cut(s) 843
Psp5II RGGWCCY 1 cut(s) 431
Psp6I CCWGG 6 cut(s) 132, 277, 427, 433, 1167, 1315
PspFI CCCAGC 3 cut(s) 102, 546, 1148
PspGI CCWGG 6 cut(s) 132, 277, 427, 433, 1167, 1315
PspN4I GGNNCC 3 cut(s) 890, 1154, 1242
PspPI GGNCC 3 cut(s) 40, 164, 431
PspPPI RGGWCCY 1 cut(s) 431
PstNI CAGNNNCTG 3 cut(s) 102, 434, 731
PsuI RGATCY 3 cut(s) 6, 341, 1240
RsaI GTAC 5 cut(s) 370, 890, 1154, 1321, 1337
RsaNI GTAC 5 cut(s) 369, 889, 1153, 1320, 1336
RseI CAYNNNNRTG 1 cut(s) 504
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 6 cut(s) 531, 611, 732, 905, 1004, 1025
Sau3AI GATC 3 cut(s) 6, 341, 1240
Sau96I GGNCC 3 cut(s) 40, 164, 431
ScaI AGTACT 1 cut(s) 1337
SchI GAGTC 2 cut(s) 632, 1324
ScrFI CCNGG 6 cut(s) 134, 279, 429, 435, 1169, 1317
SexAI ACCWGGT 1 cut(s) 277
SfaNI GCATC 8 cut(s) 21, 99, 208, 311, 407, 622, 952, 1108
SfcI CTRYAG 1 cut(s) 618
SinI GGWCC 2 cut(s) 164, 431
SmiMI CAYNNNNRTG 1 cut(s) 504
Sse9I AATT 7 cut(s) 79, 147, 443, 487, 920, 1184, 1296
SsiI CCGC 5 cut(s) 10, 137, 268, 530, 904
SspI AATATT 1 cut(s) 67
SspMI CTAG 5 cut(s) 20, 50, 381, 803, 1339
StyD4I CCNGG 6 cut(s) 132, 277, 427, 433, 1167, 1315
StyI CCWWGG 1 cut(s) 19
TaaI ACNGT 1 cut(s) 1219
TaiI ACGT 2 cut(s) 75, 694
TaqI TCGA 2 cut(s) 851, 1123
TasI AATT 7 cut(s) 79, 147, 443, 487, 920, 1184, 1296
TatI WGTACW 2 cut(s) 368, 1335
TauI GCSGC 2 cut(s) 533, 907
TfiI GAWTC 4 cut(s) 482, 566, 644, 968
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TseI GCWGC 4 cut(s) 610, 731, 1003, 1024
TspDTI ATGAA 4 cut(s) 486, 501, 509, 1197
TspGWI ACGGA 1 cut(s) 1289
Van91I CCANNNNNTGG 1 cut(s) 434
VpaK11BI GGWCC 2 cut(s) 164, 431
XapI RAATTY 2 cut(s) 443, 1296
XceI RCATGY 1 cut(s) 229
XcmI CCANNNNNNNNNTGG 1 cut(s) 1096
XmaJI CCTAGG 1 cut(s) 19
XspI CTAG 5 cut(s) 20, 50, 381, 803, 1339
ZrmI AGTACT 1 cut(s) 1337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.