Rh2DG440200

CCR4-NOT transcription complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003483
Physical Location & Seq
Forward (+)
63911107 .. 63929679
18573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG440200.1

Sequence Viewer

Length: 690 bp
ATGAGAATACATATCGAATCTGGAGTGTCCTCACAGCCACCACCTTCACCTGCTGTAACTGAACGTCTTGGAAGTCTCTCAGAACCTTCCCTCACCACAGGGGCTGCATTGGAAAAATACCAGATTGTTGCACAGAAGGGAGTCATTGGTGAAGTGCCTGATATTATACTCAGATGTGTTAGTCGAGATGAGGCCGCCTTGGCTGTGGCTCAAAAGGTTTTCAAGGGTTTGTACGAGAATGCAACAAACCAGATTCATGTTGGCGCACATCTAGCAATGCTGACTGCGATTCGTGATGTTTGCAAGCTTGTCTTTACGCAGCTCACTAGTTGGGTATGCACTATTACACTGGCAGATATTGTTTACGCAGTCCGGCCCTCCCCTTTGAATCAAGGGACTCGATCAGAGAAGCATTCTATTGAGAGGTACCAGAGAGACCAGCGGCTATACAATAACCAATTGGAGGAAACCACTCTATGCTCTCTCCTTCCTGATTGTGATGCTGATTCTGATTTCACAAGGCAAAATGACATTGCCTTGCAGCTACCAGAGCTGAGAAAGCTGCTTCAGGTGTTGAGAGAGGAGAGAGAGGGAAGGAGGGTGTGGTGGAGGCACGTGTGGGCCTGGAAATGTCTTCTTAGTGGGCCTGGGCCTGGGGACCTTGAACTCCTGACATTGAAGGCTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CNOT1_CAF1_bind CNOT1_HEAT CNOT1_TTP_bind DUF3819 Not1

Protein Analysis

229

Amino Acids

25.65

Weight (kDa)

6.53

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_Cnot1 PF25097 52 - 115 3.4e-13 Cnot1 ARM repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 58
Acc36I ACCTGC 1 cut(s) 58
Acc65I GGTACC 1 cut(s) 426
AccB1I GGYRCC 1 cut(s) 426
AciI CCGC 2 cut(s) 195, 442
AcuI CTGAAG 1 cut(s) 551
AcvI CACGTG 1 cut(s) 616
AfaI GTAC 2 cut(s) 233, 428
AfiI CCNNNNNNNGG 2 cut(s) 463, 653
AflIII ACRYGT 1 cut(s) 615
AgsI TTSAA 4 cut(s) 223, 388, 665, 679
AhlI ACTAGT 1 cut(s) 326
AjnI CCWGG 3 cut(s) 623, 646, 652
AluBI AGCT 5 cut(s) 307, 322, 544, 553, 562
AluI AGCT 5 cut(s) 307, 322, 544, 553, 562
Alw26I GTCTC 2 cut(s) 80, 429
AlwNI CAGNNNCTG 1 cut(s) 104
AoxI GGCC 5 cut(s) 192, 374, 621, 644, 650
ApeKI GCWGC 4 cut(s) 104, 319, 541, 562
Asp718I GGTACC 1 cut(s) 426
AspLEI GCGC 1 cut(s) 266
AspS9I GGNCC 5 cut(s) 375, 621, 644, 650, 658
AsuHPI GGTGA 3 cut(s) 39, 85, 161
AvaII GGWCC 1 cut(s) 658
BanI GGYRCC 1 cut(s) 426
BbrPI CACGTG 1 cut(s) 616
BbsI GAAGAC 1 cut(s) 626
BbvI GCAGC 4 cut(s) 91, 331, 549, 553
BciT130I CCWGG 3 cut(s) 625, 648, 654
BcoDI GTCTC 2 cut(s) 80, 429
BcuI ACTAGT 1 cut(s) 326
BfaI CTAG 2 cut(s) 272, 327
BfuAI ACCTGC 1 cut(s) 58
BglI GCCNNNNNGGC 1 cut(s) 200
BisI GCNGC 6 cut(s) 105, 195, 320, 443, 542, 563
BlsI GCNGC 6 cut(s) 106, 196, 321, 444, 543, 564
Bme1390I CCNGG 3 cut(s) 625, 648, 654
Bme18I GGWCC 1 cut(s) 658
BmgT120I GGNCC 5 cut(s) 375, 621, 644, 650, 658
BmiI GGNNCC 2 cut(s) 428, 659
BmrFI CCNGG 3 cut(s) 625, 648, 654
BmsI GCATC 1 cut(s) 490
BpiI GAAGAC 1 cut(s) 626
BpmI CTGGAG 1 cut(s) 42
BsaAI YACGTR 1 cut(s) 616
BsaI GGTCTC 1 cut(s) 429
BsaJI CCNNGG 3 cut(s) 198, 647, 653
Bsc4I CCNNNNNNNGG 2 cut(s) 463, 653
Bse1I ACTGG 1 cut(s) 354
Bse3DI GCAATG 2 cut(s) 282, 531
BseBI CCWGG 3 cut(s) 625, 648, 654
BseDI CCNNGG 3 cut(s) 198, 647, 653
BseLI CCNNNNNNNGG 2 cut(s) 463, 653
BseMI GCAATG 2 cut(s) 282, 531
BseMII CTCAG 3 cut(s) 93, 184, 545
BseNI ACTGG 1 cut(s) 354
BseRI GAGGAG 1 cut(s) 596
BseXI GCAGC 4 cut(s) 91, 331, 549, 553
BshFI GGCC 5 cut(s) 194, 376, 623, 646, 652
BshNI GGYRCC 1 cut(s) 426
BsiSI CCGG 1 cut(s) 373
BslFI GGGAC 2 cut(s) 409, 671
BslI CCNNNNNNNGG 2 cut(s) 463, 653
BsmAI GTCTC 2 cut(s) 80, 429
BsmFI GGGAC 2 cut(s) 409, 671
BsmI GAATGC 2 cut(s) 244, 412
BsnI GGCC 5 cut(s) 194, 376, 623, 646, 652
Bso31I GGTCTC 1 cut(s) 429
Bsp143I GATC 1 cut(s) 401
BspACI CCGC 2 cut(s) 195, 442
BspANI GGCC 5 cut(s) 194, 376, 623, 646, 652
BspCNI CTCAG 3 cut(s) 92, 183, 546
BspLI GGNNCC 2 cut(s) 428, 659
BspMI ACCTGC 1 cut(s) 58
BspT107I GGYRCC 1 cut(s) 426
BspTNI GGTCTC 1 cut(s) 429
BsrDI GCAATG 2 cut(s) 282, 531
BsrI ACTGG 1 cut(s) 354
BssECI CCNNGG 3 cut(s) 198, 647, 653
BssMI GATC 1 cut(s) 401
BssT1I CCWWGG 1 cut(s) 198
Bst2UI CCWGG 3 cut(s) 625, 648, 654
BstBAI YACGTR 1 cut(s) 616
BstC8I GCNNGC 1 cut(s) 305
BstDEI CTNAG 4 cut(s) 79, 170, 554, 638
BstHHI GCGC 1 cut(s) 266
BstKTI GATC 1 cut(s) 404
BstMAI GTCTC 2 cut(s) 80, 429
BstMBI GATC 1 cut(s) 401
BstMWI GCNNNNNNNGC 4 cut(s) 200, 272, 550, 559
BstNI CCWGG 3 cut(s) 625, 648, 654
BstSCI CCNGG 3 cut(s) 623, 646, 652
BstV1I GCAGC 4 cut(s) 91, 331, 549, 553
BstV2I GAAGAC 1 cut(s) 626
BsuRI GGCC 5 cut(s) 194, 376, 623, 646, 652
BtsIMutI CAGTG 1 cut(s) 347
BveI ACCTGC 1 cut(s) 58
Cac8I GCNNGC 1 cut(s) 305
CaiI CAGNNNCTG 1 cut(s) 104
CfoI GCGC 1 cut(s) 266
Cfr13I GGNCC 5 cut(s) 375, 621, 644, 650, 658
Csp6I GTAC 2 cut(s) 232, 427
CviAII CATG 1 cut(s) 257
CviQI GTAC 2 cut(s) 232, 427
DdeI CTNAG 4 cut(s) 79, 170, 554, 638
DpnI GATC 1 cut(s) 403
DpnII GATC 1 cut(s) 401
Eco130I CCWWGG 1 cut(s) 198
Eco31I GGTCTC 1 cut(s) 429
Eco47I GGWCC 1 cut(s) 658
Eco57I CTGAAG 1 cut(s) 551
Eco72I CACGTG 1 cut(s) 616
EcoO109I RGGNCCY 1 cut(s) 658
EcoRII CCWGG 3 cut(s) 623, 646, 652
EcoT14I CCWWGG 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 198
FaeI CATG 1 cut(s) 260
FaiI YATR 7 cut(s) 12, 167, 258, 337, 448, 478, 688
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FaqI GGGAC 2 cut(s) 409, 671
FatI CATG 1 cut(s) 256
Fnu4HI GCNGC 6 cut(s) 105, 195, 320, 443, 542, 563
Fsp4HI GCNGC 6 cut(s) 105, 195, 320, 443, 542, 563
FspBI CTAG 2 cut(s) 272, 327
GlaI GCGC 1 cut(s) 265
GluI GCNGC 6 cut(s) 105, 195, 320, 443, 542, 563
GsuI CTGGAG 1 cut(s) 42
HaeIII GGCC 5 cut(s) 194, 376, 623, 646, 652
HapII CCGG 1 cut(s) 373
HhaI GCGC 1 cut(s) 266
Hin1II CATG 1 cut(s) 260
Hin6I GCGC 1 cut(s) 264
HinP1I GCGC 1 cut(s) 264
HindIII AAGCTT 1 cut(s) 305
HinfI GANTC 7 cut(s) 17, 141, 253, 289, 388, 397, 506
HpaII CCGG 1 cut(s) 373
HphI GGTGA 3 cut(s) 39, 85, 161
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 4 cut(s) 82, 173, 406, 511
Hpy188III TCNNGA 5 cut(s) 21, 185, 293, 491, 670
Hpy8I GTNNAC 1 cut(s) 364
HpyAV CCTTC 6 cut(s) 54, 96, 130, 497, 588, 673
HpyCH4IV ACGT 2 cut(s) 64, 615
HpyCH4V TGCA 6 cut(s) 107, 131, 242, 303, 339, 541
HpyF10VI GCNNNNNNNGC 4 cut(s) 200, 272, 550, 559
HpyF3I CTNAG 4 cut(s) 79, 170, 554, 638
HpySE526I ACGT 2 cut(s) 64, 615
Hsp92II CATG 1 cut(s) 260
HspAI GCGC 1 cut(s) 264
KpnI GGTACC 1 cut(s) 430
Kzo9I GATC 1 cut(s) 401
Lsp1109I GCAGC 4 cut(s) 91, 331, 549, 553
LweI GCATC 1 cut(s) 490
MaeI CTAG 2 cut(s) 272, 327
MaeII ACGT 2 cut(s) 64, 615
MaeIII GTNAC 1 cut(s) 55
MalI GATC 1 cut(s) 403
MboI GATC 1 cut(s) 401
MboII GAAGA 1 cut(s) 626
MfeI CAATTG 1 cut(s) 458
MluCI AATT 1 cut(s) 458
MlyI GAGTC 2 cut(s) 150, 391
MspA1I CMGCKG 1 cut(s) 442
MspI CCGG 1 cut(s) 373
MspR9I CCNGG 3 cut(s) 625, 648, 654
MunI CAATTG 1 cut(s) 458
Mva1269I GAATGC 2 cut(s) 244, 412
MvaI CCWGG 3 cut(s) 625, 648, 654
MwoI GCNNNNNNNGC 4 cut(s) 200, 272, 550, 559
NdeII GATC 1 cut(s) 401
NlaIII CATG 1 cut(s) 260
NlaIV GGNNCC 2 cut(s) 428, 659
PaqCI CACCTGC 1 cut(s) 58
PctI GAATGC 2 cut(s) 244, 412
PfeI GAWTC 5 cut(s) 17, 253, 289, 388, 506
PkrI GCNGC 6 cut(s) 106, 196, 321, 444, 543, 564
PleI GAGTC 2 cut(s) 149, 391
PmaCI CACGTG 1 cut(s) 616
PmlI CACGTG 1 cut(s) 616
PpsI GAGTC 2 cut(s) 149, 391
Ppu21I YACGTR 1 cut(s) 616
PpuMI RGGWCCY 1 cut(s) 658
Psp5II RGGWCCY 1 cut(s) 658
Psp6I CCWGG 3 cut(s) 623, 646, 652
PspCI CACGTG 1 cut(s) 616
PspGI CCWGG 3 cut(s) 623, 646, 652
PspN4I GGNNCC 2 cut(s) 428, 659
PspPI GGNCC 5 cut(s) 375, 621, 644, 650, 658
PspPPI RGGWCCY 1 cut(s) 658
PstNI CAGNNNCTG 1 cut(s) 104
RsaI GTAC 2 cut(s) 233, 428
RsaNI GTAC 2 cut(s) 232, 427
SatI GCNGC 6 cut(s) 105, 195, 320, 443, 542, 563
Sau3AI GATC 1 cut(s) 401
Sau96I GGNCC 5 cut(s) 375, 621, 644, 650, 658
SchI GAGTC 2 cut(s) 150, 391
ScrFI CCNGG 3 cut(s) 625, 648, 654
SfaNI GCATC 1 cut(s) 490
SinI GGWCC 1 cut(s) 658
SpeI ACTAGT 1 cut(s) 326
Sse9I AATT 1 cut(s) 458
SsiI CCGC 2 cut(s) 195, 442
SspMI CTAG 2 cut(s) 272, 327
StyD4I CCNGG 3 cut(s) 623, 646, 652
StyI CCWWGG 1 cut(s) 198
TaiI ACGT 2 cut(s) 67, 618
TaqI TCGA 3 cut(s) 15, 184, 400
TasI AATT 1 cut(s) 458
TauI GCSGC 2 cut(s) 197, 445
TfiI GAWTC 5 cut(s) 17, 253, 289, 388, 506
TscAI CASTG 1 cut(s) 354
TseI GCWGC 4 cut(s) 104, 319, 541, 562
TspDTI ATGAA 1 cut(s) 245
TspRI CASTG 1 cut(s) 354
VpaK11BI GGWCC 1 cut(s) 658
XcmI CCANNNNNNNNNTGG 1 cut(s) 257
XspI CTAG 2 cut(s) 272, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.