Rh4DG160500

CCR4-NOT transcription complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0003483
Physical Location & Seq
Reverse (-)
31924803 .. 31926991
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG160500.1

Sequence Viewer

Length: 570 bp
ATGTTGTATACTGTCCGTGAGGTTTCTTTGCCAGGTGGGAAAACAGAGGAGGGAGACAAGGATGATGGTGACACAGCAACAAGAGAAGCGAAAGAGGAGATTGGTTTGGATCCTAAGCTTGTGAATGTTGTCACTGTTCATGAACCATTTTTGTCAAAGCACCTACTGAGAGTTGTACCTATTATCGGTATACTTAACGATAAGGAAGCATTCAAGCCTGCTCCAAATCCGGCCGAAGTGGAATCAGTATTTGATGCTCCCTTGGAAATGTTCATCAAGTTGGAAGCTCTTGTGACTAACGATGCTAGAGAATCAGAAATCCAGGTTTTCAAGGGTTTGTACGAGAATGCAACAAACCAGATTCATGTTGGCGCACATCTAGCAATGCTGACTGCGATTCGTGATGTTTGCAAGCTTGTCTTTACGCAGCTCACTAGTTGGGTGATTTATTCTGATGAGGAACGGAAGTTTAATAAAGAAATTACTGTTGGCCTTATACGCAGTGAACTGCTAAACCTTGCAGAGTACAATGTTCACATGGAAAAACTTATTGATGGTGGCAAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CNOT1_CAF1_bind CNOT1_HEAT CNOT1_TTP_bind DUF3819 Not1

Protein Analysis

189

Amino Acids

21.15

Weight (kDa)

4.94

Isoelectric Point (pI)

25.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 9 - 87 1.6e-08 NUDIX domain
ARM_Cnot1 PF25097 108 - 188 9.7e-25 Cnot1 ARM repeat
NOT1_connector PF23590 122 - 188 3.5e-06 CCR4-NOT transcription complex subunit 1, NOT1_connector
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 8, 190
AclWI GGATC 2 cut(s) 104, 117
AcoI YGGCCR 1 cut(s) 231
AfaI GTAC 3 cut(s) 177, 341, 527
AfiI CCNNNNNNNGG 1 cut(s) 185
AgsI TTSAA 2 cut(s) 214, 331
AhlI ACTAGT 1 cut(s) 434
AjnI CCWGG 2 cut(s) 31, 321
AjuI GAANNNNNNNTTGG 2 cut(s) 471, 503
AluBI AGCT 4 cut(s) 118, 287, 415, 430
AluI AGCT 4 cut(s) 118, 287, 415, 430
Alw26I GTCTC 1 cut(s) 48
AlwI GGATC 2 cut(s) 104, 117
AoxI GGCC 2 cut(s) 231, 490
ApeKI GCWGC 1 cut(s) 427
AspLEI GCGC 1 cut(s) 374
AsuHPI GGTGA 2 cut(s) 80, 454
BamHI GGATCC 1 cut(s) 109
BbvI GCAGC 1 cut(s) 439
BccI CCATC 2 cut(s) 59, 548
BciT130I CCWGG 2 cut(s) 33, 323
BcoDI GTCTC 1 cut(s) 48
BcuI ACTAGT 1 cut(s) 434
BfaI CTAG 3 cut(s) 306, 380, 435
BisI GCNGC 1 cut(s) 428
BlsI GCNGC 1 cut(s) 429
Bme1390I CCNGG 2 cut(s) 33, 323
BmiI GGNNCC 1 cut(s) 111
BmrFI CCNGG 2 cut(s) 33, 323
BmsI GCATC 2 cut(s) 244, 292
Bpu10I CCTNAGC 1 cut(s) 114
BsaJI CCNNGG 1 cut(s) 261
Bsc4I CCNNNNNNNGG 1 cut(s) 185
Bse3DI GCAATG 1 cut(s) 390
BseBI CCWGG 2 cut(s) 33, 323
BseDI CCNNGG 1 cut(s) 261
BseGI GGATG 1 cut(s) 67
BseLI CCNNNNNNNGG 1 cut(s) 185
BseMI GCAATG 1 cut(s) 390
BseMII CTCAG 1 cut(s) 158
BseRI GAGGAG 2 cut(s) 62, 110
BseX3I CGGCCG 1 cut(s) 231
BseXI GCAGC 1 cut(s) 439
Bsh1285I CGRYCG 1 cut(s) 234
BshFI GGCC 2 cut(s) 233, 492
BsiEI CGRYCG 1 cut(s) 234
BsiSI CCGG 1 cut(s) 230
BslI CCNNNNNNNGG 1 cut(s) 185
BsmAI GTCTC 1 cut(s) 48
BsmI GAATGC 2 cut(s) 209, 352
BsnI GGCC 2 cut(s) 233, 492
Bsp143I GATC 1 cut(s) 109
BspANI GGCC 2 cut(s) 233, 492
BspCNI CTCAG 1 cut(s) 159
BspHI TCATGA 1 cut(s) 139
BspLI GGNNCC 1 cut(s) 111
BspPI GGATC 2 cut(s) 104, 117
BsrDI GCAATG 1 cut(s) 390
BssECI CCNNGG 1 cut(s) 261
BssMI GATC 1 cut(s) 109
BssNAI GTATAC 2 cut(s) 9, 191
BssT1I CCWWGG 1 cut(s) 261
Bst1107I GTATAC 2 cut(s) 9, 191
Bst2UI CCWGG 2 cut(s) 33, 323
Bst4CI ACNGT 3 cut(s) 13, 136, 487
BstC8I GCNNGC 2 cut(s) 219, 413
BstDEI CTNAG 2 cut(s) 114, 167
BstF5I GGATG 1 cut(s) 67
BstHHI GCGC 1 cut(s) 374
BstKTI GATC 1 cut(s) 112
BstMAI GTCTC 1 cut(s) 48
BstMBI GATC 1 cut(s) 109
BstMCI CGRYCG 1 cut(s) 234
BstMWI GCNNNNNNNGC 2 cut(s) 380, 498
BstNI CCWGG 2 cut(s) 33, 323
BstSCI CCNGG 2 cut(s) 31, 321
BstV1I GCAGC 1 cut(s) 439
BstX2I RGATCY 1 cut(s) 109
BstYI RGATCY 1 cut(s) 109
BstZ17I GTATAC 2 cut(s) 9, 191
BstZI CGGCCG 1 cut(s) 231
BsuRI GGCC 2 cut(s) 233, 492
BtsCI GGATG 1 cut(s) 67
BtsI GCAGTG 1 cut(s) 508
BtsIMutI CAGTG 2 cut(s) 132, 508
Cac8I GCNNGC 2 cut(s) 219, 413
CciI TCATGA 1 cut(s) 139
CfoI GCGC 1 cut(s) 374
Csp6I GTAC 3 cut(s) 176, 340, 526
CviAII CATG 3 cut(s) 140, 365, 538
CviJI RGCY 7 cut(s) 118, 217, 233, 287, 415, 430, 492
CviKI_1 RGCY 7 cut(s) 118, 217, 233, 287, 415, 430, 492
CviQI GTAC 3 cut(s) 176, 340, 526
DdeI CTNAG 2 cut(s) 114, 167
DpnI GATC 1 cut(s) 111
DpnII GATC 1 cut(s) 109
EaeI YGGCCR 1 cut(s) 231
EagI CGGCCG 1 cut(s) 231
EclXI CGGCCG 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 261
Eco52I CGGCCG 1 cut(s) 231
EcoRII CCWGG 2 cut(s) 31, 321
EcoT14I CCWWGG 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 3 cut(s) 143, 368, 541
FaiI YATR 6 cut(s) 9, 141, 191, 366, 497, 539
FalI AAGNNNNNCTT 2 cut(s) 404, 436
FatI CATG 3 cut(s) 139, 364, 537
FblI GTMKAC 2 cut(s) 8, 190
Fnu4HI GCNGC 1 cut(s) 428
FokI GGATG 1 cut(s) 74
Fsp4HI GCNGC 1 cut(s) 428
FspBI CTAG 3 cut(s) 306, 380, 435
GlaI GCGC 1 cut(s) 373
GluI GCNGC 1 cut(s) 428
HaeIII GGCC 2 cut(s) 233, 492
HapII CCGG 1 cut(s) 230
HhaI GCGC 1 cut(s) 374
Hin1II CATG 3 cut(s) 143, 368, 541
Hin6I GCGC 1 cut(s) 372
HinP1I GCGC 1 cut(s) 372
HindIII AAGCTT 2 cut(s) 116, 413
HinfI GANTC 4 cut(s) 242, 311, 361, 397
HpaII CCGG 1 cut(s) 230
HphI GGTGA 2 cut(s) 80, 454
Hpy166II GTNNAC 4 cut(s) 9, 191, 506, 535
Hpy188I TCNGA 2 cut(s) 316, 454
Hpy188III TCNNGA 2 cut(s) 140, 401
Hpy8I GTNNAC 4 cut(s) 9, 191, 506, 535
HpyCH4III ACNGT 3 cut(s) 13, 136, 487
HpyCH4V TGCA 3 cut(s) 350, 411, 521
HpyF10VI GCNNNNNNNGC 2 cut(s) 380, 498
HpyF3I CTNAG 2 cut(s) 114, 167
Hsp92II CATG 3 cut(s) 143, 368, 541
HspAI GCGC 1 cut(s) 372
Kzo9I GATC 1 cut(s) 109
LmnI GCTCC 2 cut(s) 226, 262
LpnPI CCDG 7 cut(s) 18, 45, 231, 243, 308, 335, 371
Lsp1109I GCAGC 1 cut(s) 439
LweI GCATC 2 cut(s) 244, 292
MaeI CTAG 3 cut(s) 306, 380, 435
MaeIII GTNAC 3 cut(s) 68, 130, 292
MalI GATC 1 cut(s) 111
MboI GATC 1 cut(s) 109
MflI RGATCY 1 cut(s) 109
MluCI AATT 1 cut(s) 480
MmeI TCCRAC 1 cut(s) 261
MnlI CCTC 5 cut(s) 13, 40, 43, 88, 451
MseI TTAA 3 cut(s) 195, 471, 568
MspI CCGG 1 cut(s) 230
MspR9I CCNGG 2 cut(s) 33, 323
Mva1269I GAATGC 2 cut(s) 209, 352
MvaI CCWGG 2 cut(s) 33, 323
MwoI GCNNNNNNNGC 2 cut(s) 380, 498
NdeII GATC 1 cut(s) 109
NlaIII CATG 3 cut(s) 143, 368, 541
NlaIV GGNNCC 1 cut(s) 111
NmuCI GTSAC 3 cut(s) 68, 130, 292
PagI TCATGA 1 cut(s) 139
PctI GAATGC 2 cut(s) 209, 352
PfeI GAWTC 4 cut(s) 242, 311, 361, 397
PkrI GCNGC 1 cut(s) 429
Psp6I CCWGG 2 cut(s) 31, 321
PspGI CCWGG 2 cut(s) 31, 321
PspN4I GGNNCC 1 cut(s) 111
PsuI RGATCY 1 cut(s) 109
RsaI GTAC 3 cut(s) 177, 341, 527
RsaNI GTAC 3 cut(s) 176, 340, 526
SaqAI TTAA 3 cut(s) 195, 471, 568
SatI GCNGC 1 cut(s) 428
Sau3AI GATC 1 cut(s) 109
ScrFI CCNGG 2 cut(s) 33, 323
SfaNI GCATC 2 cut(s) 244, 292
SpeI ACTAGT 1 cut(s) 434
Sse9I AATT 1 cut(s) 480
SspMI CTAG 3 cut(s) 306, 380, 435
StyD4I CCNGG 2 cut(s) 31, 321
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 3 cut(s) 13, 136, 487
TasI AATT 1 cut(s) 480
TatI WGTACW 1 cut(s) 525
TfiI GAWTC 4 cut(s) 242, 311, 361, 397
Tru1I TTAA 3 cut(s) 195, 471, 568
Tru9I TTAA 3 cut(s) 195, 471, 568
TscAI CASTG 2 cut(s) 139, 508
TseFI GTSAC 3 cut(s) 68, 130, 292
TseI GCWGC 1 cut(s) 427
Tsp45I GTSAC 3 cut(s) 68, 130, 292
TspDTI ATGAA 4 cut(s) 128, 156, 262, 353
TspGWI ACGGA 2 cut(s) 5, 478
TspRI CASTG 2 cut(s) 139, 508
XcmI CCANNNNNNNNNTGG 1 cut(s) 365
XmiI GTMKAC 2 cut(s) 8, 190
XspI CTAG 3 cut(s) 306, 380, 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.