Rh4AG049800

CCR4-NOT transcription complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003483
Physical Location & Seq
Forward (+)
10418513 .. 10421360
2848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG049800.1

Sequence Viewer

Length: 387 bp
ATGACGATGAATATCGCCATTAACCTCCATCAAGATAGGGCAAACGGCTGCACCATCCACCACAATGAGGAGAAGAAGATGTCTGATATTATACTCAGATGTGTTAGTCGAGATGAGGCCGCCTTGGCTGTGGCTCAAAAGGTTTTCAAGGGTTTGTACGAGAATGCAACAAACCAGATTCATGTTGGCGCACATCTAGCAATGCTGACTGGGATTCGTGATGTTTGCAAGCTTGTCTTTACGCAGCTCACTAGTTGGGTGATTTATTCTGATGAGGAGCGGAAGTTTAATAAAGAAATTATTGTCGGCCTTATACGCAGTGAACTGCTAAACCTTGCAGAGTACAATGTTCACATGGCAAAACTTATTGATGGTGGCAAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CNOT1_CAF1_bind CNOT1_HEAT CNOT1_TTP_bind DUF3819 Not1

Protein Analysis

128

Amino Acids

14.45

Weight (kDa)

7.01

Isoelectric Point (pI)

31.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_Cnot1 PF25097 27 - 127 6e-34 Cnot1 ARM repeat
NOT1_connector PF23590 31 - 127 4.3e-09 CCR4-NOT transcription complex subunit 1, NOT1_connector
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 280
AciI CCGC 2 cut(s) 120, 280
AfaI GTAC 2 cut(s) 158, 344
AfiI CCNNNNNNNGG 1 cut(s) 67
AgsI TTSAA 1 cut(s) 148
AhlI ACTAGT 1 cut(s) 251
AluBI AGCT 2 cut(s) 232, 247
AluI AGCT 2 cut(s) 232, 247
AoxI GGCC 2 cut(s) 117, 307
ApeKI GCWGC 2 cut(s) 48, 244
AspLEI GCGC 1 cut(s) 191
AsuHPI GGTGA 1 cut(s) 271
BbvI GCAGC 2 cut(s) 35, 256
BccI CCATC 3 cut(s) 36, 62, 365
BceAI ACGGC 1 cut(s) 61
BcuI ACTAGT 1 cut(s) 251
BfaI CTAG 2 cut(s) 197, 252
BglI GCCNNNNNGGC 1 cut(s) 125
BisI GCNGC 3 cut(s) 49, 120, 245
BlsI GCNGC 3 cut(s) 50, 121, 246
BmrI ACTGGG 1 cut(s) 219
BmuI ACTGGG 1 cut(s) 219
BsaBI GATNNNNATC 1 cut(s) 11
BsaJI CCNNGG 1 cut(s) 123
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse1I ACTGG 1 cut(s) 214
Bse3DI GCAATG 1 cut(s) 207
Bse8I GATNNNNATC 1 cut(s) 11
BseDI CCNNGG 1 cut(s) 123
BseGI GGATG 1 cut(s) 54
BseJI GATNNNNATC 1 cut(s) 11
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMI GCAATG 1 cut(s) 207
BseMII CTCAG 1 cut(s) 109
BseNI ACTGG 1 cut(s) 214
BseRI GAGGAG 2 cut(s) 83, 290
BseXI GCAGC 2 cut(s) 35, 256
BsgI GTGCAG 1 cut(s) 34
BshFI GGCC 2 cut(s) 119, 309
BslI CCNNNNNNNGG 1 cut(s) 67
BsmI GAATGC 1 cut(s) 169
BsnI GGCC 2 cut(s) 119, 309
BspACI CCGC 2 cut(s) 120, 280
BspANI GGCC 2 cut(s) 119, 309
BspCNI CTCAG 1 cut(s) 108
BsrBI CCGCTC 1 cut(s) 280
BsrDI GCAATG 1 cut(s) 207
BsrI ACTGG 1 cut(s) 214
BssECI CCNNGG 1 cut(s) 123
BssT1I CCWWGG 1 cut(s) 123
BstC8I GCNNGC 1 cut(s) 230
BstDEI CTNAG 1 cut(s) 95
BstF5I GGATG 1 cut(s) 54
BstHHI GCGC 1 cut(s) 191
BstMWI GCNNNNNNNGC 3 cut(s) 125, 197, 315
BstV1I GCAGC 2 cut(s) 35, 256
BsuRI GGCC 2 cut(s) 119, 309
BtsCI GGATG 1 cut(s) 54
BtsI GCAGTG 1 cut(s) 325
BtsIMutI CAGTG 1 cut(s) 325
Cac8I GCNNGC 1 cut(s) 230
CfoI GCGC 1 cut(s) 191
Csp6I GTAC 2 cut(s) 157, 343
CviAII CATG 2 cut(s) 182, 355
CviJI RGCY 7 cut(s) 48, 119, 128, 134, 232, 247, 309
CviKI_1 RGCY 7 cut(s) 48, 119, 128, 134, 232, 247, 309
CviQI GTAC 2 cut(s) 157, 343
DdeI CTNAG 1 cut(s) 95
Eco130I CCWWGG 1 cut(s) 123
EcoT14I CCWWGG 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 123
FaeI CATG 2 cut(s) 185, 358
FaiI YATR 4 cut(s) 92, 183, 314, 356
FalI AAGNNNNNCTT 2 cut(s) 221, 253
FatI CATG 2 cut(s) 181, 354
Fnu4HI GCNGC 3 cut(s) 49, 120, 245
FokI GGATG 1 cut(s) 41
Fsp4HI GCNGC 3 cut(s) 49, 120, 245
FspBI CTAG 2 cut(s) 197, 252
GlaI GCGC 1 cut(s) 190
GluI GCNGC 3 cut(s) 49, 120, 245
HaeIII GGCC 2 cut(s) 119, 309
HhaI GCGC 1 cut(s) 191
Hin1II CATG 2 cut(s) 185, 358
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 2 cut(s) 178, 214
HphI GGTGA 1 cut(s) 271
Hpy166II GTNNAC 2 cut(s) 323, 352
Hpy188I TCNGA 3 cut(s) 85, 98, 271
Hpy188III TCNNGA 3 cut(s) 32, 110, 218
Hpy8I GTNNAC 2 cut(s) 323, 352
HpyCH4V TGCA 4 cut(s) 51, 167, 228, 338
HpyF10VI GCNNNNNNNGC 3 cut(s) 125, 197, 315
HpyF3I CTNAG 1 cut(s) 95
Hsp92II CATG 2 cut(s) 185, 358
HspAI GCGC 1 cut(s) 189
LmnI GCTCC 1 cut(s) 277
LpnPI CCDG 2 cut(s) 188, 195
Lsp1109I GCAGC 2 cut(s) 35, 256
MaeI CTAG 2 cut(s) 197, 252
MbiI CCGCTC 1 cut(s) 280
MboII GAAGA 2 cut(s) 85, 88
MluCI AATT 1 cut(s) 297
MnlI CCTC 4 cut(s) 35, 61, 109, 268
MseI TTAA 3 cut(s) 21, 288, 385
MslI CAYNNNNRTG 1 cut(s) 63
Mva1269I GAATGC 1 cut(s) 169
MwoI GCNNNNNNNGC 3 cut(s) 125, 197, 315
NlaIII CATG 2 cut(s) 185, 358
PctI GAATGC 1 cut(s) 169
PfeI GAWTC 2 cut(s) 178, 214
PkrI GCNGC 3 cut(s) 50, 121, 246
RsaI GTAC 2 cut(s) 158, 344
RsaNI GTAC 2 cut(s) 157, 343
RseI CAYNNNNRTG 1 cut(s) 63
SaqAI TTAA 3 cut(s) 21, 288, 385
SatI GCNGC 3 cut(s) 49, 120, 245
SetI ASST 5 cut(s) 27, 144, 234, 249, 336
SmiMI CAYNNNNRTG 1 cut(s) 63
SpeI ACTAGT 1 cut(s) 251
Sse9I AATT 1 cut(s) 297
SsiI CCGC 2 cut(s) 120, 280
SspMI CTAG 2 cut(s) 197, 252
StyI CCWWGG 1 cut(s) 123
TaqI TCGA 1 cut(s) 109
TasI AATT 1 cut(s) 297
TatI WGTACW 1 cut(s) 342
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 2 cut(s) 178, 214
Tru1I TTAA 3 cut(s) 21, 288, 385
Tru9I TTAA 3 cut(s) 21, 288, 385
TscAI CASTG 1 cut(s) 325
TseI GCWGC 2 cut(s) 48, 244
TspDTI ATGAA 2 cut(s) 23, 170
TspRI CASTG 1 cut(s) 325
XcmI CCANNNNNNNNNTGG 1 cut(s) 182
XspI CTAG 2 cut(s) 197, 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.