Rmu_sc0000466.1_g000013

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000466.1
OG0002274
Physical Location & Seq
Reverse (-)
55206 .. 56291
1086 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 996 bp
atgggagggtgtttttccaacgtgaaaggtggccagcaagccgtgggaggggtccgtcatcaagagaggcccatcaacagggacgctgactcaaacgacgccgtcgagctctttaaccactcccaaggggtttttccacaccatactccgctcgaggagccaagtcatgctgccgacccacagctactgccatctgctccaccaccaccaatacttcctggagaagaagaagaggatgattttgaagagtgccaaatggatatggcgattagggaccagttggatgaagcaccaaacttcagttttacttcttctgtgccaactcatggcgaagcttcacagccactaccgtctgctccaccactgggagtaattcctgaagaagaagacatcgatgatgaagaagcgcgtcaaatagctatggcgattatggtctctttagagcatgggcggaatgtaccaactggaactgcacagccactaccatctgctccaccactgggagtaattcctgaagatgaagacaccgatgatgaagaagcgcgtcaaatagctatggcgattatggtctctttggagcatgggcgggatgtaccaactggaactgcacagccactaccatctgctccaccacagggagtaattcctgaagatgaagacaccgacgatgaagaagcgcgtcaaatagctatggcgattatggtctctctggagcatgggctgaatgtaccaactggaactgcatctagcagtgatacacctttgcaacgaggtcaaactacaggttcacagtcactgtcatctgctcaacaaactccaaacggttctggtagcagcccagaatgccagtattgttatgatgctccgatcgaagcaacttgtgatccctgtgggcatttagcttgctttaactgctggctgaagattcaagcttctgacaacaagtgcccttgttgtcgggtagtgatcaagaagatcatcaagctctatatgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

331

Amino Acids

35.44

Weight (kDa)

4.25

Isoelectric Point (pI)

60.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 280 - 325 2.8e-09 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 326
AccBSI CCGCTC 1 cut(s) 151
AccII CGCG 3 cut(s) 409, 544, 679
AciI CCGC 3 cut(s) 149, 451, 586
AclWI GGATC 1 cut(s) 878
AcoI YGGCCR 1 cut(s) 31
AcuI CTGAAG 5 cut(s) 283, 399, 534, 669, 941
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 3 cut(s) 459, 594, 729
AfiI CCNNNNNNNGG 6 cut(s) 48, 78, 326, 365, 500, 635
AgsI TTSAA 2 cut(s) 245, 929
AjnI CCWGG 1 cut(s) 217
AjuI GAANNNNNNNTTGG 2 cut(s) 117, 149
AluBI AGCT 9 cut(s) 109, 184, 335, 419, 554, 689, 902, 932, 985
AluI AGCT 9 cut(s) 109, 184, 335, 419, 554, 689, 902, 932, 985
Alw21I GWGCWC 1 cut(s) 111
Alw26I GTCTC 3 cut(s) 439, 574, 709
AlwI GGATC 1 cut(s) 878
AlwNI CAGNNNCTG 2 cut(s) 187, 796
Ama87I CYCGRG 1 cut(s) 152
AoxI GGCC 2 cut(s) 31, 68
ApeKI GCWGC 2 cut(s) 170, 834
AspLEI GCGC 3 cut(s) 409, 544, 679
AspS9I GGNCC 3 cut(s) 52, 69, 274
AvaI CYCGRG 1 cut(s) 152
AvaII GGWCC 2 cut(s) 52, 274
BaeGI GKGCMC 1 cut(s) 950
BalI TGGCCA 1 cut(s) 33
BanII GRGCYC 1 cut(s) 111
BbsI GAAGAC 3 cut(s) 393, 528, 663
Bbv12I GWGCWC 1 cut(s) 111
BbvI GCAGC 2 cut(s) 157, 846
BccI CCATC 4 cut(s) 80, 199, 493, 628
BceAI ACGGC 2 cut(s) 26, 86
BciT130I CCWGG 1 cut(s) 219
BclI TGATCA 1 cut(s) 966
BcoDI GTCTC 3 cut(s) 439, 574, 709
BfaI CTAG 1 cut(s) 747
BfmI CTRYAG 1 cut(s) 780
BisI GCNGC 2 cut(s) 171, 835
BlsI GCNGC 2 cut(s) 172, 836
Bme1390I CCNGG 1 cut(s) 219
Bme18I GGWCC 2 cut(s) 52, 274
BmeT110I CYCGRG 1 cut(s) 152
BmgT120I GGNCC 3 cut(s) 52, 69, 274
BmiI GGNNCC 3 cut(s) 53, 159, 275
BmrFI CCNGG 1 cut(s) 219
BmrI ACTGGG 2 cut(s) 374, 509
BmsI GCATC 2 cut(s) 752, 850
BmuI ACTGGG 2 cut(s) 374, 509
BpiI GAAGAC 3 cut(s) 393, 528, 663
BpmI CTGGAG 2 cut(s) 240, 731
Bsa29I ATCGAT 1 cut(s) 393
BsaHI GRCGYC 1 cut(s) 99
BsaI GGTCTC 3 cut(s) 439, 574, 709
BsaJI CCNNGG 2 cut(s) 42, 124
Bsc4I CCNNNNNNNGG 6 cut(s) 48, 78, 326, 365, 500, 635
Bse1I ACTGG 7 cut(s) 277, 369, 469, 504, 604, 739, 847
BseBI CCWGG 1 cut(s) 219
BseCI ATCGAT 1 cut(s) 393
BseDI CCNNGG 2 cut(s) 42, 124
BseGI GGATG 3 cut(s) 241, 289, 595
BseLI CCNNNNNNNGG 6 cut(s) 48, 78, 326, 365, 500, 635
BseNI ACTGG 7 cut(s) 277, 369, 469, 504, 604, 739, 847
BseRI GAGGAG 1 cut(s) 170
BseSI GKGCMC 1 cut(s) 950
BseXI GCAGC 2 cut(s) 157, 846
BsgI GTGCAG 2 cut(s) 456, 591
Bsh1236I CGCG 3 cut(s) 409, 544, 679
Bsh1285I CGRYCG 1 cut(s) 870
BshFI GGCC 2 cut(s) 33, 70
BshVI ATCGAT 1 cut(s) 393
BsiEI CGRYCG 1 cut(s) 870
BsiHKAI GWGCWC 1 cut(s) 111
BsiHKCI CYCGRG 1 cut(s) 152
BslFI GGGAC 2 cut(s) 95, 287
BslI CCNNNNNNNGG 6 cut(s) 48, 78, 326, 365, 500, 635
BsmAI GTCTC 3 cut(s) 439, 574, 709
BsmFI GGGAC 2 cut(s) 95, 287
BsmI GAATGC 1 cut(s) 848
BsnI GGCC 2 cut(s) 33, 70
Bso31I GGTCTC 3 cut(s) 439, 574, 709
BsoBI CYCGRG 1 cut(s) 152
Bsp1286I GDGCHC 2 cut(s) 111, 950
Bsp143I GATC 4 cut(s) 867, 883, 966, 975
BspACI CCGC 3 cut(s) 149, 451, 586
BspANI GGCC 2 cut(s) 33, 70
BspDI ATCGAT 1 cut(s) 393
BspFNI CGCG 3 cut(s) 409, 544, 679
BspLI GGNNCC 3 cut(s) 53, 159, 275
BspPI GGATC 1 cut(s) 878
BspTNI GGTCTC 3 cut(s) 439, 574, 709
BsrBI CCGCTC 1 cut(s) 151
BsrI ACTGG 7 cut(s) 277, 369, 469, 504, 604, 739, 847
BssECI CCNNGG 2 cut(s) 42, 124
BssMI GATC 4 cut(s) 867, 883, 966, 975
BssNI GRCGYC 1 cut(s) 99
BssT1I CCWWGG 1 cut(s) 124
Bst2UI CCWGG 1 cut(s) 219
Bst4CI ACNGT 4 cut(s) 351, 792, 798, 824
Bst6I CTCTTC 2 cut(s) 225, 240
BstACI GRCGYC 1 cut(s) 99
BstC8I GCNNGC 4 cut(s) 35, 39, 904, 917
BstDSI CCRYGG 1 cut(s) 42
BstF5I GGATG 3 cut(s) 241, 289, 595
BstFNI CGCG 3 cut(s) 409, 544, 679
BstHHI GCGC 3 cut(s) 409, 544, 679
BstKTI GATC 4 cut(s) 870, 886, 969, 978
BstMAI GTCTC 3 cut(s) 439, 574, 709
BstMBI GATC 4 cut(s) 867, 883, 966, 975
BstMCI CGRYCG 1 cut(s) 870
BstMWI GCNNNNNNNGC 3 cut(s) 157, 843, 912
BstNI CCWGG 1 cut(s) 219
BstSCI CCNGG 1 cut(s) 217
BstSFI CTRYAG 1 cut(s) 780
BstSLI GKGCMC 1 cut(s) 950
BstUI CGCG 3 cut(s) 409, 544, 679
BstV1I GCAGC 2 cut(s) 157, 846
BstV2I GAAGAC 3 cut(s) 393, 528, 663
Bsu15I ATCGAT 1 cut(s) 393
BsuRI GGCC 2 cut(s) 33, 70
BsuTUI ATCGAT 1 cut(s) 393
BtgI CCRYGG 1 cut(s) 42
BtsCI GGATG 3 cut(s) 241, 289, 595
BtsI GCAGTG 1 cut(s) 757
BtsIMutI CAGTG 4 cut(s) 362, 497, 757, 794
Cac8I GCNNGC 4 cut(s) 35, 39, 904, 917
CaiI CAGNNNCTG 2 cut(s) 187, 796
CfoI GCGC 3 cut(s) 409, 544, 679
Cfr13I GGNCC 3 cut(s) 52, 69, 274
ClaI ATCGAT 1 cut(s) 393
CseI GACGC 5 cut(s) 92, 107, 398, 533, 668
Csp6I GTAC 3 cut(s) 458, 593, 728
CviAII CATG 5 cut(s) 167, 326, 446, 581, 716
CviQI GTAC 3 cut(s) 458, 593, 728
DpnI GATC 4 cut(s) 869, 885, 968, 977
DpnII GATC 4 cut(s) 867, 883, 966, 975
EaeI YGGCCR 1 cut(s) 31
Eam1104I CTCTTC 2 cut(s) 225, 240
EarI CTCTTC 2 cut(s) 225, 240
EciI GGCGGA 1 cut(s) 466
Ecl136II GAGCTC 1 cut(s) 109
Eco130I CCWWGG 1 cut(s) 124
Eco24I GRGCYC 1 cut(s) 111
Eco31I GGTCTC 3 cut(s) 439, 574, 709
Eco47I GGWCC 2 cut(s) 52, 274
Eco53kI GAGCTC 1 cut(s) 109
Eco57I CTGAAG 5 cut(s) 283, 399, 534, 669, 941
Eco88I CYCGRG 1 cut(s) 152
EcoICRI GAGCTC 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 217
EcoT14I CCWWGG 1 cut(s) 124
EcoT38I GRGCYC 1 cut(s) 111
ErhI CCWWGG 1 cut(s) 124
FaeI CATG 5 cut(s) 170, 329, 449, 584, 719
FaqI GGGAC 2 cut(s) 95, 287
FatI CATG 5 cut(s) 166, 325, 445, 580, 715
FauI CCCGC 1 cut(s) 579
FbaI TGATCA 1 cut(s) 966
Fnu4HI GCNGC 2 cut(s) 171, 835
FokI GGATG 3 cut(s) 248, 296, 602
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 2 cut(s) 171, 835
FspBI CTAG 1 cut(s) 747
GlaI GCGC 3 cut(s) 408, 543, 678
GluI GCNGC 2 cut(s) 171, 835
GsuI CTGGAG 2 cut(s) 240, 731
HaeIII GGCC 2 cut(s) 33, 70
HgaI GACGC 5 cut(s) 92, 107, 398, 533, 668
HhaI GCGC 3 cut(s) 409, 544, 679
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 5 cut(s) 170, 329, 449, 584, 719
Hin6I GCGC 3 cut(s) 407, 542, 677
HinP1I GCGC 3 cut(s) 407, 542, 677
HindIII AAGCTT 2 cut(s) 333, 930
HinfI GANTC 2 cut(s) 89, 925
Hpy166II GTNNAC 1 cut(s) 788
Hpy188I TCNGA 2 cut(s) 867, 937
Hpy188III TCNNGA 6 cut(s) 62, 377, 512, 647, 710, 970
Hpy8I GTNNAC 1 cut(s) 788
Hpy99I CGWCG 3 cut(s) 101, 107, 668
HpyCH4III ACNGT 4 cut(s) 351, 792, 798, 824
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 4 cut(s) 473, 608, 743, 766
HpyF10VI GCNNNNNNNGC 3 cut(s) 157, 843, 912
HpySE526I ACGT 1 cut(s) 21
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 5 cut(s) 170, 329, 449, 584, 719
HspAI GCGC 3 cut(s) 407, 542, 677
Ksp22I TGATCA 1 cut(s) 966
Kzo9I GATC 4 cut(s) 867, 883, 966, 975
LmnI GCTCC 8 cut(s) 157, 202, 361, 496, 577, 631, 712, 868
Lsp1109I GCAGC 2 cut(s) 157, 846
LweI GCATC 2 cut(s) 752, 850
MaeI CTAG 1 cut(s) 747
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 1 cut(s) 792
MalI GATC 4 cut(s) 869, 885, 968, 977
MbiI CCGCTC 1 cut(s) 151
MboI GATC 4 cut(s) 867, 883, 966, 975
MhlI GDGCHC 2 cut(s) 111, 950
MlsI TGGCCA 1 cut(s) 33
MluCI AATT 3 cut(s) 372, 507, 642
MluNI TGGCCA 1 cut(s) 33
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 2 cut(s) 42, 261
MnlI CCTC 5 cut(s) 41, 60, 148, 226, 764
Mox20I TGGCCA 1 cut(s) 33
MscI TGGCCA 1 cut(s) 33
MseI TTAA 2 cut(s) 114, 909
Msp20I TGGCCA 1 cut(s) 33
MspR9I CCNGG 1 cut(s) 219
Mva1269I GAATGC 1 cut(s) 848
MvaI CCWGG 1 cut(s) 219
MvnI CGCG 3 cut(s) 409, 544, 679
MwoI GCNNNNNNNGC 3 cut(s) 157, 843, 912
NdeII GATC 4 cut(s) 867, 883, 966, 975
NlaIII CATG 5 cut(s) 170, 329, 449, 584, 719
NlaIV GGNNCC 3 cut(s) 53, 159, 275
NmuCI GTSAC 1 cut(s) 792
PaeR7I CTCGAG 1 cut(s) 152
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PctI GAATGC 1 cut(s) 848
PfeI GAWTC 1 cut(s) 925
PflFI GACNNNGTC 1 cut(s) 101
PflMI CCANNNNNTGG 1 cut(s) 326
PfoI TCCNGGA 1 cut(s) 217
PkrI GCNGC 2 cut(s) 172, 836
Ple19I CGATCG 1 cut(s) 870
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
Psp124BI GAGCTC 1 cut(s) 111
Psp6I CCWGG 1 cut(s) 217
PspGI CCWGG 1 cut(s) 217
PspN4I GGNNCC 3 cut(s) 53, 159, 275
PspPI GGNCC 3 cut(s) 52, 69, 274
PspXI VCTCGAGB 1 cut(s) 152
PstNI CAGNNNCTG 2 cut(s) 187, 796
PsyI GACNNNGTC 1 cut(s) 101
PvuI CGATCG 1 cut(s) 870
RsaI GTAC 3 cut(s) 459, 594, 729
RsaNI GTAC 3 cut(s) 458, 593, 728
SacI GAGCTC 1 cut(s) 111
SaqAI TTAA 2 cut(s) 114, 909
SatI GCNGC 2 cut(s) 171, 835
Sau3AI GATC 4 cut(s) 867, 883, 966, 975
Sau96I GGNCC 3 cut(s) 52, 69, 274
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 1 cut(s) 219
SduI GDGCHC 2 cut(s) 111, 950
SfaNI GCATC 2 cut(s) 752, 850
SfcI CTRYAG 1 cut(s) 780
Sfr274I CTCGAG 1 cut(s) 152
SinI GGWCC 2 cut(s) 52, 274
SlaI CTCGAG 1 cut(s) 152
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 3 cut(s) 372, 507, 642
SsiI CCGC 3 cut(s) 149, 451, 586
SspMI CTAG 1 cut(s) 747
SstI GAGCTC 1 cut(s) 111
StyD4I CCNGG 1 cut(s) 217
StyI CCWWGG 1 cut(s) 124
TaaI ACNGT 4 cut(s) 351, 792, 798, 824
TaiI ACGT 1 cut(s) 24
TaqI TCGA 4 cut(s) 105, 153, 393, 870
TasI AATT 3 cut(s) 372, 507, 642
TfiI GAWTC 1 cut(s) 925
Tru1I TTAA 2 cut(s) 114, 909
Tru9I TTAA 2 cut(s) 114, 909
TscAI CASTG 4 cut(s) 369, 504, 757, 801
TseFI GTSAC 1 cut(s) 792
TseI GCWGC 2 cut(s) 170, 834
Tsp45I GTSAC 1 cut(s) 792
TspDTI ATGAA 6 cut(s) 300, 414, 534, 549, 669, 684
TspGWI ACGGA 1 cut(s) 44
TspRI CASTG 4 cut(s) 369, 504, 757, 801
Tth111I GACNNNGTC 1 cut(s) 101
Van91I CCANNNNNTGG 1 cut(s) 326
VpaK11BI GGWCC 2 cut(s) 52, 274
XhoI CTCGAG 1 cut(s) 152
XspI CTAG 1 cut(s) 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.