Rmu_sc0001246.1_g000004

4F5 protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001246.1
OG0002526
Physical Location & Seq
Forward (+)
13938 .. 17486
3549 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 450 bp
atggaaaagaaaaatggtcaccggcgtggagaacaagaattggggtttcgggtcgatctggattggttagccaacgtggcgctactgatgcagcagaatacgatgatcattagattggtaggttctgaacaactcgtcctttatatacccacccacctcttctcactcgcggacacattcaaccttgctctaatagacgaaggattgcacccatctgtgctcgcatcggcaatcagcgaacgcggtaaccagagagaccgagatcgcgaaagggctcaggccaggtccggcaacaagaccaagcagcccaaaaccgatggattaacccctgaacaacgccgagaaagggacgccaaggcgctgcaggaaaaagcggcaaagaaggcggcgcaggcgcaggcggccggagggaacaaagcgggaggaatcaatgccggcggcaagaagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4F5

Protein Analysis

149

Amino Acids

16.37

Weight (kDa)

9.92

Isoelectric Point (pI)

34.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SERF-like_N PF04419 81 - 114 1.3e-07 Small EDRK-rich factor 1/2-like, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 170, 243, 267
AciI CCGC 7 cut(s) 170, 243, 374, 386, 401, 419, 438
AcoI YGGCCR 1 cut(s) 402
AcyI GRCGYC 1 cut(s) 351
AfiI CCNNNNNNNGG 1 cut(s) 346
AgsI TTSAA 1 cut(s) 181
AjnI CCWGG 1 cut(s) 281
AleI CACNNNNGTG 1 cut(s) 24
AloI GAACNNNNNNTCC 2 cut(s) 120, 152
Alw21I GWGCWC 1 cut(s) 222
Alw26I GTCTC 1 cut(s) 249
AoxI GGCC 2 cut(s) 279, 402
ApeKI GCWGC 3 cut(s) 91, 304, 361
AspLEI GCGC 4 cut(s) 82, 361, 391, 397
AspS9I GGNCC 1 cut(s) 285
AsuHPI GGTGA 1 cut(s) 11
AvaII GGWCC 1 cut(s) 285
BanII GRGCYC 1 cut(s) 277
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 3 cut(s) 103, 316, 348
BccI CCATC 2 cut(s) 220, 311
BciT130I CCWGG 1 cut(s) 283
BclI TGATCA 1 cut(s) 105
BcoDI GTCTC 1 cut(s) 249
BfmI CTRYAG 1 cut(s) 362
BfoI RGCGCY 2 cut(s) 83, 362
BglI GCCNNNNNGGC 1 cut(s) 77
BisI GCNGC 7 cut(s) 92, 305, 362, 375, 387, 402, 439
BlsI GCNGC 7 cut(s) 93, 306, 363, 376, 388, 403, 440
Bme1390I CCNGG 1 cut(s) 283
Bme18I GGWCC 1 cut(s) 285
BmgT120I GGNCC 1 cut(s) 285
BmrFI CCNGG 1 cut(s) 283
BmsI GCATC 2 cut(s) 78, 233
Bpu10I CCTNAGC 1 cut(s) 276
BsaHI GRCGYC 1 cut(s) 351
BsaI GGTCTC 1 cut(s) 249
BsaJI CCNNGG 1 cut(s) 354
Bsc4I CCNNNNNNNGG 1 cut(s) 346
Bse118I RCCGGY 2 cut(s) 21, 434
BseBI CCWGG 1 cut(s) 283
BseDI CCNNGG 1 cut(s) 354
BseLI CCNNNNNNNGG 1 cut(s) 346
BseMII CTCAG 1 cut(s) 290
BseX3I CGGCCG 1 cut(s) 402
BseXI GCAGC 3 cut(s) 103, 316, 348
Bsh1236I CGCG 3 cut(s) 170, 243, 267
Bsh1285I CGRYCG 1 cut(s) 405
BshFI GGCC 2 cut(s) 281, 404
BsiEI CGRYCG 1 cut(s) 405
BsiHKAI GWGCWC 1 cut(s) 222
BsiSI CCGG 4 cut(s) 22, 288, 405, 435
BslFI GGGAC 1 cut(s) 362
BslI CCNNNNNNNGG 1 cut(s) 346
BsmAI GTCTC 1 cut(s) 249
BsmFI GGGAC 1 cut(s) 362
BsnI GGCC 2 cut(s) 281, 404
Bso31I GGTCTC 1 cut(s) 249
Bsp1286I GDGCHC 2 cut(s) 222, 277
Bsp143I GATC 3 cut(s) 55, 105, 262
Bsp68I TCGCGA 1 cut(s) 267
BspACI CCGC 7 cut(s) 170, 243, 374, 386, 401, 419, 438
BspANI GGCC 2 cut(s) 281, 404
BspCNI CTCAG 1 cut(s) 289
BspFNI CGCG 3 cut(s) 170, 243, 267
BspMAI CTGCAG 1 cut(s) 366
BspTNI GGTCTC 1 cut(s) 249
BsrFI RCCGGY 2 cut(s) 21, 434
BssAI RCCGGY 2 cut(s) 21, 434
BssECI CCNNGG 1 cut(s) 354
BssMI GATC 3 cut(s) 55, 105, 262
BssNI GRCGYC 1 cut(s) 351
BssT1I CCWWGG 1 cut(s) 354
Bst2UI CCWGG 1 cut(s) 283
Bst6I CTCTTC 1 cut(s) 164
BstACI GRCGYC 1 cut(s) 351
BstC8I GCNNGC 4 cut(s) 222, 393, 399, 436
BstDEI CTNAG 1 cut(s) 276
BstEII GGTNACC 2 cut(s) 17, 245
BstFNI CGCG 3 cut(s) 170, 243, 267
BstH2I RGCGCY 2 cut(s) 83, 362
BstHHI GCGC 4 cut(s) 82, 361, 391, 397
BstKTI GATC 3 cut(s) 58, 108, 265
BstMAI GTCTC 1 cut(s) 249
BstMBI GATC 3 cut(s) 55, 105, 262
BstMCI CGRYCG 1 cut(s) 405
BstMWI GCNNNNNNNGC 5 cut(s) 77, 88, 383, 392, 401
BstNI CCWGG 1 cut(s) 283
BstPI GGTNACC 2 cut(s) 17, 245
BstSCI CCNGG 1 cut(s) 281
BstSFI CTRYAG 1 cut(s) 362
BstUI CGCG 3 cut(s) 170, 243, 267
BstV1I GCAGC 3 cut(s) 103, 316, 348
BstZI CGGCCG 1 cut(s) 402
BsuRI GGCC 2 cut(s) 281, 404
BtuMI TCGCGA 1 cut(s) 267
Cac8I GCNNGC 4 cut(s) 222, 393, 399, 436
CfoI GCGC 4 cut(s) 82, 361, 391, 397
Cfr10I RCCGGY 2 cut(s) 21, 434
Cfr13I GGNCC 1 cut(s) 285
CseI GACGC 1 cut(s) 359
CviJI RGCY 5 cut(s) 71, 275, 281, 307, 404
CviKI_1 RGCY 5 cut(s) 71, 275, 281, 307, 404
DdeI CTNAG 1 cut(s) 276
DpnI GATC 3 cut(s) 57, 107, 264
DpnII GATC 3 cut(s) 55, 105, 262
EaeI YGGCCR 1 cut(s) 402
EagI CGGCCG 1 cut(s) 402
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
EclXI CGGCCG 1 cut(s) 402
Eco130I CCWWGG 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 277
Eco31I GGTCTC 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 285
Eco52I CGGCCG 1 cut(s) 402
Eco91I GGTNACC 2 cut(s) 17, 245
EcoO65I GGTNACC 2 cut(s) 17, 245
EcoRII CCWGG 1 cut(s) 281
EcoT14I CCWWGG 1 cut(s) 354
EcoT38I GRGCYC 1 cut(s) 277
ErhI CCWWGG 1 cut(s) 354
FaiI YATR 2 cut(s) 144, 146
FaqI GGGAC 1 cut(s) 362
FauI CCCGC 1 cut(s) 412
FbaI TGATCA 1 cut(s) 105
Fnu4HI GCNGC 7 cut(s) 92, 305, 362, 375, 387, 402, 439
FriOI GRGCYC 1 cut(s) 277
Fsp4HI GCNGC 7 cut(s) 92, 305, 362, 375, 387, 402, 439
GlaI GCGC 4 cut(s) 81, 360, 390, 396
GluI GCNGC 7 cut(s) 92, 305, 362, 375, 387, 402, 439
HaeII RGCGCY 2 cut(s) 83, 362
HaeIII GGCC 2 cut(s) 281, 404
HapII CCGG 4 cut(s) 22, 288, 405, 435
HgaI GACGC 1 cut(s) 359
HhaI GCGC 4 cut(s) 82, 361, 391, 397
Hin1I GRCGYC 1 cut(s) 351
Hin6I GCGC 4 cut(s) 80, 359, 389, 395
HinP1I GCGC 4 cut(s) 80, 359, 389, 395
HinfI GANTC 1 cut(s) 426
HpaII CCGG 4 cut(s) 22, 288, 405, 435
HphI GGTGA 1 cut(s) 11
Hpy188I TCNGA 1 cut(s) 127
Hpy188III TCNNGA 2 cut(s) 59, 266
HpyAV CCTTC 2 cut(s) 194, 376
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 3 cut(s) 91, 208, 364
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 88, 383, 392, 401
HpyF3I CTNAG 1 cut(s) 276
HpySE526I ACGT 1 cut(s) 75
Hsp92I GRCGYC 1 cut(s) 351
HspAI GCGC 4 cut(s) 80, 359, 389, 395
KroI GCCGGC 1 cut(s) 434
KroNI GCCGGC 1 cut(s) 436
Ksp22I TGATCA 1 cut(s) 105
Kzo9I GATC 3 cut(s) 55, 105, 262
Lsp1109I GCAGC 3 cut(s) 103, 316, 348
LweI GCATC 2 cut(s) 78, 233
MaeII ACGT 1 cut(s) 75
MaeIII GTNAC 2 cut(s) 17, 245
MalI GATC 3 cut(s) 57, 107, 264
MboI GATC 3 cut(s) 55, 105, 262
MboII GAAGA 1 cut(s) 151
MhlI GDGCHC 2 cut(s) 222, 277
MluCI AATT 1 cut(s) 38
MnlI CCTC 3 cut(s) 167, 401, 416
MroNI GCCGGC 1 cut(s) 434
MseI TTAA 1 cut(s) 323
MslI CAYNNNNRTG 1 cut(s) 24
MspI CCGG 4 cut(s) 22, 288, 405, 435
MspR9I CCNGG 1 cut(s) 283
MvaI CCWGG 1 cut(s) 283
MvnI CGCG 3 cut(s) 170, 243, 267
MwoI GCNNNNNNNGC 5 cut(s) 77, 88, 383, 392, 401
NaeI GCCGGC 1 cut(s) 436
NdeII GATC 3 cut(s) 55, 105, 262
NgoMIV GCCGGC 1 cut(s) 434
NmeAIII GCCGAG 1 cut(s) 365
NmuCI GTSAC 1 cut(s) 17
NruI TCGCGA 1 cut(s) 267
OliI CACNNNNGTG 1 cut(s) 24
PdiI GCCGGC 1 cut(s) 436
PfeI GAWTC 1 cut(s) 426
PkrI GCNGC 7 cut(s) 93, 306, 363, 376, 388, 403, 440
Psp6I CCWGG 1 cut(s) 281
PspEI GGTNACC 2 cut(s) 17, 245
PspGI CCWGG 1 cut(s) 281
PspPI GGNCC 1 cut(s) 285
PstI CTGCAG 1 cut(s) 366
RruI TCGCGA 1 cut(s) 267
RseI CAYNNNNRTG 1 cut(s) 24
SaqAI TTAA 1 cut(s) 323
SatI GCNGC 7 cut(s) 92, 305, 362, 375, 387, 402, 439
Sau3AI GATC 3 cut(s) 55, 105, 262
Sau96I GGNCC 1 cut(s) 285
ScrFI CCNGG 1 cut(s) 283
SduI GDGCHC 2 cut(s) 222, 277
SetI ASST 5 cut(s) 78, 124, 159, 186, 287
SfaNI GCATC 2 cut(s) 78, 233
SfcI CTRYAG 1 cut(s) 362
SgrAI CRCCGGYG 1 cut(s) 21
SinI GGWCC 1 cut(s) 285
SmiMI CAYNNNNRTG 1 cut(s) 24
Sse9I AATT 1 cut(s) 38
SsiI CCGC 7 cut(s) 170, 243, 374, 386, 401, 419, 438
StyD4I CCNGG 1 cut(s) 281
StyI CCWWGG 1 cut(s) 354
TaiI ACGT 1 cut(s) 78
TaqI TCGA 1 cut(s) 54
TaqII GACCGA 1 cut(s) 273
TasI AATT 1 cut(s) 38
TauI GCSGC 4 cut(s) 377, 389, 404, 441
TfiI GAWTC 1 cut(s) 426
Tru1I TTAA 1 cut(s) 323
Tru9I TTAA 1 cut(s) 323
TseFI GTSAC 1 cut(s) 17
TseI GCWGC 3 cut(s) 91, 304, 361
Tsp45I GTSAC 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.