Rmu_sc0001317.1_g000013

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001317.1
OG0002729
Physical Location & Seq
Forward (+)
33583 .. 35420
1838 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 627 bp
atgtgtgctgaccttcgtgaggaagaagaattgtttttcattgtgtctaaggatggaattgatactggccccttctctaggcatcttcctgcaatccaaagcgaggcagaagcttgtagggtaggcttgcttgtgggtattcaccagaattggagacatgttgaggtagaaagtgattgtgctcagttgcttgctgtgctgaatcttcaaggggaggataactctgaagttagtcgaatactgattggaaagccttcaagatatccagttctagttgcggcatctgtgggaagctatggagcctatttggctgatggttccgagtatagtggcaactgtggcgatgctgtgactctagaagcattgaaagatttccacagggaaagggttcagattctggccaactctgttgctcatctaattgcatttgagacgacagcgaataaattagaggcaaagtccgctgaacttcttgaggaggaagcattagatattccagcatggtttacttttgcttctaaagttggaatcagtgtggtgagtggtgattccatttttgagtgtgcctctattgctaatgtgtggaagcaagttgttgttgttggaatctactgtacattgccttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
S-methyl_trans

Protein Analysis

208

Amino Acids

22.57

Weight (kDa)

4.62

Isoelectric Point (pI)

38.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 87 - 208 1.4e-20 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 278, 462
AcoI YGGCCR 1 cut(s) 399
AcuI CTGAAG 1 cut(s) 246
AfaI GTAC 1 cut(s) 616
AfiI CCNNNNNNNGG 3 cut(s) 19, 78, 103
AflIII ACRYGT 1 cut(s) 157
AgsI TTSAA 3 cut(s) 209, 258, 367
AluBI AGCT 2 cut(s) 113, 294
AluI AGCT 2 cut(s) 113, 294
Alw21I GWGCWC 1 cut(s) 184
Alw26I GTCTC 2 cut(s) 148, 425
AlwNI CAGNNNCTG 1 cut(s) 397
AoxI GGCC 2 cut(s) 67, 399
Asp700I GAANNNNTTC 3 cut(s) 253, 371, 387
AspS9I GGNCC 1 cut(s) 68
AsuHPI GGTGA 3 cut(s) 134, 550, 557
BalI TGGCCA 1 cut(s) 401
Bbv12I GWGCWC 1 cut(s) 184
BccI CCATC 2 cut(s) 47, 308
BcoDI GTCTC 2 cut(s) 148, 425
BfaI CTAG 3 cut(s) 78, 272, 356
BglI GCCNNNNNGGC 1 cut(s) 308
BisI GCNGC 1 cut(s) 279
BlsI GCNGC 1 cut(s) 280
BmgT120I GGNCC 1 cut(s) 68
BmiI GGNNCC 3 cut(s) 70, 301, 319
BmsI GCATC 3 cut(s) 91, 290, 334
BplI GAGNNNNNCTC 4 cut(s) 206, 238, 551, 583
BpuEI CTTGAG 1 cut(s) 494
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 78, 103
Bse1I ACTGG 2 cut(s) 70, 266
Bse3DI GCAATG 1 cut(s) 617
BseGI GGATG 1 cut(s) 58
BseLI CCNNNNNNNGG 3 cut(s) 19, 78, 103
BseMI GCAATG 1 cut(s) 617
BseMII CTCAG 1 cut(s) 197
BseNI ACTGG 2 cut(s) 70, 266
BseRI GAGGAG 1 cut(s) 491
BshFI GGCC 2 cut(s) 69, 401
BsiHKAI GWGCWC 1 cut(s) 184
BslI CCNNNNNNNGG 3 cut(s) 19, 78, 103
BsmAI GTCTC 2 cut(s) 148, 425
BsmBI CGTCTC 1 cut(s) 425
BsnI GGCC 2 cut(s) 69, 401
Bsp1286I GDGCHC 1 cut(s) 184
Bsp1407I TGTACA 1 cut(s) 614
BspACI CCGC 2 cut(s) 278, 462
BspANI GGCC 2 cut(s) 69, 401
BspCNI CTCAG 1 cut(s) 196
BspLI GGNNCC 3 cut(s) 70, 301, 319
BsrDI GCAATG 1 cut(s) 617
BsrGI TGTACA 1 cut(s) 614
BsrI ACTGG 2 cut(s) 70, 266
Bst4CI ACNGT 2 cut(s) 338, 614
BstAUI TGTACA 1 cut(s) 614
BstC8I GCNNGC 2 cut(s) 128, 192
BstDEI CTNAG 2 cut(s) 48, 183
BstENI CCTNNNNNAGG 2 cut(s) 17, 76
BstF5I GGATG 1 cut(s) 58
BstMAI GTCTC 2 cut(s) 148, 425
BstMWI GCNNNNNNNGC 5 cut(s) 196, 308, 339, 461, 572
BstNSI RCATGY 1 cut(s) 161
BsuRI GGCC 2 cut(s) 69, 401
BtgZI GCGATG 1 cut(s) 357
BtsCI GGATG 1 cut(s) 58
BtsIMutI CAGTG 1 cut(s) 538
Cac8I GCNNGC 2 cut(s) 128, 192
CaiI CAGNNNCTG 1 cut(s) 397
Cfr13I GGNCC 1 cut(s) 68
Csp6I GTAC 1 cut(s) 615
CviAII CATG 2 cut(s) 158, 501
CviJI RGCY 8 cut(s) 69, 113, 126, 253, 294, 302, 311, 401
CviKI_1 RGCY 8 cut(s) 69, 113, 126, 253, 294, 302, 311, 401
CviQI GTAC 1 cut(s) 615
DdeI CTNAG 2 cut(s) 48, 183
EaeI YGGCCR 1 cut(s) 399
Eco32I GATATC 1 cut(s) 263
Eco57I CTGAAG 1 cut(s) 246
EcoNI CCTNNNNNAGG 2 cut(s) 17, 76
EcoRV GATATC 1 cut(s) 263
Esp3I CGTCTC 1 cut(s) 425
FaeI CATG 2 cut(s) 161, 504
FaiI YATR 4 cut(s) 159, 297, 327, 502
FatI CATG 2 cut(s) 157, 500
Fnu4HI GCNGC 1 cut(s) 279
FokI GGATG 1 cut(s) 65
Fsp4HI GCNGC 1 cut(s) 279
FspBI CTAG 3 cut(s) 78, 272, 356
GluI GCNGC 1 cut(s) 279
HaeIII GGCC 2 cut(s) 69, 401
Hin1II CATG 2 cut(s) 161, 504
HindIII AAGCTT 1 cut(s) 111
HinfI GANTC 6 cut(s) 202, 352, 394, 528, 548, 606
HphI GGTGA 3 cut(s) 134, 550, 557
Hpy166II GTNNAC 1 cut(s) 507
Hpy188I TCNGA 3 cut(s) 226, 322, 393
Hpy188III TCNNGA 4 cut(s) 17, 258, 356, 473
Hpy8I GTNNAC 1 cut(s) 507
HpyAV CCTTC 3 cut(s) 23, 82, 264
HpyCH4III ACNGT 2 cut(s) 338, 614
HpyCH4V TGCA 2 cut(s) 92, 425
HpyF10VI GCNNNNNNNGC 5 cut(s) 196, 308, 339, 461, 572
HpyF3I CTNAG 2 cut(s) 48, 183
Hsp92II CATG 2 cut(s) 161, 504
LmnI GCTCC 1 cut(s) 299
LpnPI CCDG 7 cut(s) 51, 102, 158, 279, 364, 383, 510
LweI GCATC 3 cut(s) 91, 290, 334
MaeI CTAG 3 cut(s) 78, 272, 356
MaeIII GTNAC 1 cut(s) 349
MboII GAAGA 4 cut(s) 35, 38, 77, 197
MhlI GDGCHC 1 cut(s) 184
MlsI TGGCCA 1 cut(s) 401
MluCI AATT 5 cut(s) 29, 57, 148, 420, 446
MluNI TGGCCA 1 cut(s) 401
MlyI GAGTC 1 cut(s) 346
MmeI TCCRAC 2 cut(s) 505, 583
MnlI CCTC 8 cut(s) 13, 97, 157, 208, 445, 469, 472, 577
Mox20I TGGCCA 1 cut(s) 401
MroXI GAANNNNTTC 3 cut(s) 253, 371, 387
MscI TGGCCA 1 cut(s) 401
Msp20I TGGCCA 1 cut(s) 401
MspA1I CMGCKG 1 cut(s) 464
MwoI GCNNNNNNNGC 5 cut(s) 196, 308, 339, 461, 572
NlaIII CATG 2 cut(s) 161, 504
NlaIV GGNNCC 3 cut(s) 70, 301, 319
NmuCI GTSAC 1 cut(s) 349
NspI RCATGY 1 cut(s) 161
PciI ACATGT 1 cut(s) 157
PdmI GAANNNNTTC 3 cut(s) 253, 371, 387
PfeI GAWTC 5 cut(s) 202, 394, 528, 548, 606
PkrI GCNGC 1 cut(s) 280
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
PscI ACATGT 1 cut(s) 157
PspN4I GGNNCC 3 cut(s) 70, 301, 319
PspPI GGNCC 1 cut(s) 68
PstNI CAGNNNCTG 1 cut(s) 397
RsaI GTAC 1 cut(s) 616
RsaNI GTAC 1 cut(s) 615
SatI GCNGC 1 cut(s) 279
Sau96I GGNCC 1 cut(s) 68
SchI GAGTC 1 cut(s) 346
SduI GDGCHC 1 cut(s) 184
SetI ASST 4 cut(s) 15, 115, 168, 296
SfaNI GCATC 3 cut(s) 91, 290, 334
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
Sse9I AATT 5 cut(s) 29, 57, 148, 420, 446
SsiI CCGC 2 cut(s) 278, 462
SspMI CTAG 3 cut(s) 78, 272, 356
TaaI ACNGT 2 cut(s) 338, 614
TaqI TCGA 1 cut(s) 235
TasI AATT 5 cut(s) 29, 57, 148, 420, 446
TatI WGTACW 1 cut(s) 614
TauI GCSGC 1 cut(s) 281
TfiI GAWTC 5 cut(s) 202, 394, 528, 548, 606
TscAI CASTG 1 cut(s) 538
TseFI GTSAC 1 cut(s) 349
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 1 cut(s) 28
TspRI CASTG 1 cut(s) 538
XagI CCTNNNNNAGG 2 cut(s) 17, 76
XbaI TCTAGA 1 cut(s) 355
XceI RCATGY 1 cut(s) 161
XmnI GAANNNNTTC 3 cut(s) 253, 371, 387
XspI CTAG 3 cut(s) 78, 272, 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.