Rmu_sc0001544.1_g000023

Zinc knuckle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001544.1
OG0004260
Physical Location & Seq
Reverse (-)
99792 .. 100232
441 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 441 bp
atggccgaggctatggcggctagattggctacagcacttgcactgacagacgaagcgatggttgagtttggggaggctggagcaacggctgatgcagcgtcgggtttttacttggccgttgggagtatgttggcgcctagggctgaggatgatgctggattgcccaaagagctggcggcggtgtggggcctgtgtgatcgcctttcgatcatgaaggttgacagtgaccgattccaattccggtttgcggggaagaaggagggacaaagggttctacacggcggcctgtggcatttcaagaactgcattcttctgttggaagagtatgacggattcggagcgccggagatggtttcgctgaactcactagaggtgtgggtcattgtgaggcgtttgccaccaagtctgaggactgaagaatccttgatgctgatcgaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

146

Amino Acids

15.96

Weight (kDa)

4.79

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 54 - 110 2.2e-08 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 133
AciI CCGC 5 cut(s) 17, 176, 179, 248, 282
AcoI YGGCCR 2 cut(s) 3, 114
AcuI CTGAAG 1 cut(s) 435
AcyI GRCGYC 1 cut(s) 134
AfiI CCNNNNNNNGG 1 cut(s) 247
AgsI TTSAA 1 cut(s) 298
AloI GAACNNNNNNTCC 2 cut(s) 255, 287
AluBI AGCT 1 cut(s) 172
AluI AGCT 1 cut(s) 172
AoxI GGCC 4 cut(s) 3, 114, 187, 283
ApeKI GCWGC 1 cut(s) 95
AspA2I CCTAGG 1 cut(s) 137
AspLEI GCGC 2 cut(s) 136, 343
AspS9I GGNCC 1 cut(s) 187
AvrII CCTAGG 1 cut(s) 137
BanI GGYRCC 1 cut(s) 133
BbvCI CCTCAGC 1 cut(s) 144
BbvI GCAGC 1 cut(s) 107
BccI CCATC 2 cut(s) 52, 343
BceAI ACGGC 3 cut(s) 101, 102, 295
BfaI CTAG 3 cut(s) 21, 138, 368
BfmI CTRYAG 1 cut(s) 30
BfoI RGCGCY 2 cut(s) 137, 344
BisI GCNGC 4 cut(s) 18, 96, 177, 283
BlnI CCTAGG 1 cut(s) 137
BlsI GCNGC 4 cut(s) 19, 97, 178, 284
BmgT120I GGNCC 1 cut(s) 187
BmiI GGNNCC 2 cut(s) 135, 188
BmsI GCATC 3 cut(s) 82, 142, 417
BpmI CTGGAG 1 cut(s) 99
Bpu10I CCTNAGC 1 cut(s) 144
BsaBI GATNNNNATC 1 cut(s) 431
BsaHI GRCGYC 1 cut(s) 134
BsaJI CCNNGG 2 cut(s) 6, 137
BsaWI WCCGGW 1 cut(s) 240
Bsc4I CCNNNNNNNGG 1 cut(s) 247
Bse8I GATNNNNATC 1 cut(s) 431
BseDI CCNNGG 2 cut(s) 6, 137
BseGI GGATG 1 cut(s) 154
BseJI GATNNNNATC 1 cut(s) 431
BseLI CCNNNNNNNGG 1 cut(s) 247
BseMII CTCAG 2 cut(s) 135, 398
BseXI GCAGC 1 cut(s) 107
BshFI GGCC 4 cut(s) 5, 116, 189, 285
BshNI GGYRCC 1 cut(s) 133
BsiSI CCGG 2 cut(s) 241, 344
BslFI GGGAC 1 cut(s) 276
BslI CCNNNNNNNGG 1 cut(s) 247
BsmFI GGGAC 1 cut(s) 276
BsmI GAATGC 1 cut(s) 306
BsnI GGCC 4 cut(s) 5, 116, 189, 285
Bsp143I GATC 3 cut(s) 196, 207, 432
BspACI CCGC 5 cut(s) 17, 176, 179, 248, 282
BspANI GGCC 4 cut(s) 5, 116, 189, 285
BspCNI CTCAG 2 cut(s) 136, 399
BspHI TCATGA 1 cut(s) 210
BspLI GGNNCC 2 cut(s) 135, 188
BspT107I GGYRCC 1 cut(s) 133
BssECI CCNNGG 2 cut(s) 6, 137
BssMI GATC 3 cut(s) 196, 207, 432
BssNI GRCGYC 1 cut(s) 134
BssT1I CCWWGG 1 cut(s) 137
Bst4CI ACNGT 1 cut(s) 224
Bst6I CTCTTC 1 cut(s) 315
BstACI GRCGYC 1 cut(s) 134
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 2 cut(s) 144, 407
BstF5I GGATG 1 cut(s) 154
BstH2I RGCGCY 2 cut(s) 137, 344
BstHHI GCGC 2 cut(s) 136, 343
BstKTI GATC 3 cut(s) 199, 210, 435
BstMBI GATC 3 cut(s) 196, 207, 432
BstMWI GCNNNNNNNGC 5 cut(s) 17, 26, 95, 140, 169
BstSFI CTRYAG 1 cut(s) 30
BstV1I GCAGC 1 cut(s) 107
BstXI CCANNNNNNTGG 1 cut(s) 172
BsuRI GGCC 4 cut(s) 5, 116, 189, 285
BtgZI GCGATG 1 cut(s) 71
BtsCI GGATG 1 cut(s) 154
BtsIMutI CAGTG 2 cut(s) 41, 229
Cac8I GCNNGC 1 cut(s) 174
CciI TCATGA 1 cut(s) 210
CfoI GCGC 2 cut(s) 136, 343
Cfr13I GGNCC 1 cut(s) 187
CseI GACGC 1 cut(s) 87
CviAII CATG 1 cut(s) 211
DdeI CTNAG 2 cut(s) 144, 407
DinI GGCGCC 1 cut(s) 135
DpnI GATC 3 cut(s) 198, 209, 434
DpnII GATC 3 cut(s) 196, 207, 432
EaeI YGGCCR 2 cut(s) 3, 114
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
Eco130I CCWWGG 1 cut(s) 137
Eco57I CTGAAG 1 cut(s) 435
EcoO109I RGGNCCY 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 137
EgeI GGCGCC 1 cut(s) 135
EheI GGCGCC 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 137
FaeI CATG 1 cut(s) 214
FaiI YATR 4 cut(s) 14, 128, 212, 327
FaqI GGGAC 1 cut(s) 276
FatI CATG 1 cut(s) 210
FauI CCCGC 1 cut(s) 241
Fnu4HI GCNGC 4 cut(s) 18, 96, 177, 283
FokI GGATG 1 cut(s) 161
Fsp4HI GCNGC 4 cut(s) 18, 96, 177, 283
FspBI CTAG 3 cut(s) 21, 138, 368
GlaI GCGC 2 cut(s) 135, 342
GluI GCNGC 4 cut(s) 18, 96, 177, 283
GsuI CTGGAG 1 cut(s) 99
HaeII RGCGCY 2 cut(s) 137, 344
HaeIII GGCC 4 cut(s) 5, 116, 189, 285
HapII CCGG 2 cut(s) 241, 344
HgaI GACGC 1 cut(s) 87
HhaI GCGC 2 cut(s) 136, 343
Hin1I GRCGYC 1 cut(s) 134
Hin1II CATG 1 cut(s) 214
Hin6I GCGC 2 cut(s) 134, 341
HinP1I GCGC 2 cut(s) 134, 341
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 3 cut(s) 231, 333, 419
HpaII CCGG 2 cut(s) 241, 344
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 2 cut(s) 338, 408
Hpy188III TCNNGA 2 cut(s) 211, 298
Hpy8I GTNNAC 1 cut(s) 220
Hpy99I CGWCG 1 cut(s) 103
HpyAV CCTTC 2 cut(s) 208, 250
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4V TGCA 3 cut(s) 41, 95, 306
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 26, 95, 140, 169
HpyF3I CTNAG 2 cut(s) 144, 407
Hsp92I GRCGYC 1 cut(s) 134
Hsp92II CATG 1 cut(s) 214
HspAI GCGC 2 cut(s) 134, 341
KasI GGCGCC 1 cut(s) 133
Kzo9I GATC 3 cut(s) 196, 207, 432
LmnI GCTCC 2 cut(s) 80, 338
LpnPI CCDG 7 cut(s) 63, 141, 158, 203, 254, 299, 357
Lsp1109I GCAGC 1 cut(s) 107
LweI GCATC 3 cut(s) 82, 142, 417
MaeI CTAG 3 cut(s) 21, 138, 368
MaeIII GTNAC 1 cut(s) 224
MalI GATC 3 cut(s) 198, 209, 434
MboI GATC 3 cut(s) 196, 207, 432
MboII GAAGA 4 cut(s) 265, 302, 332, 428
MluCI AATT 1 cut(s) 236
Mly113I GGCGCC 1 cut(s) 134
MmeI TCCRAC 1 cut(s) 297
MnlI CCTC 6 cut(s) 67, 139, 253, 364, 381, 402
MspI CCGG 2 cut(s) 241, 344
Mva1269I GAATGC 1 cut(s) 306
MwoI GCNNNNNNNGC 5 cut(s) 17, 26, 95, 140, 169
NarI GGCGCC 1 cut(s) 134
NdeII GATC 3 cut(s) 196, 207, 432
NlaIII CATG 1 cut(s) 214
NlaIV GGNNCC 2 cut(s) 135, 188
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 1 cut(s) 224
PagI TCATGA 1 cut(s) 210
PctI GAATGC 1 cut(s) 306
PfeI GAWTC 3 cut(s) 231, 333, 419
PkrI GCNGC 4 cut(s) 19, 97, 178, 284
PluTI GGCGCC 1 cut(s) 137
PspN4I GGNNCC 2 cut(s) 135, 188
PspPI GGNCC 1 cut(s) 187
SatI GCNGC 4 cut(s) 18, 96, 177, 283
Sau3AI GATC 3 cut(s) 196, 207, 432
Sau96I GGNCC 1 cut(s) 187
SetI ASST 3 cut(s) 174, 219, 375
SfaNI GCATC 3 cut(s) 82, 142, 417
SfcI CTRYAG 1 cut(s) 30
SfoI GGCGCC 1 cut(s) 135
Sse9I AATT 1 cut(s) 236
SsiI CCGC 5 cut(s) 17, 176, 179, 248, 282
SspDI GGCGCC 1 cut(s) 133
SspMI CTAG 3 cut(s) 21, 138, 368
StyI CCWWGG 1 cut(s) 137
TaaI ACNGT 1 cut(s) 224
TaqI TCGA 2 cut(s) 206, 435
TaqII GACCGA 1 cut(s) 243
TasI AATT 1 cut(s) 236
TauI GCSGC 3 cut(s) 20, 179, 285
TfiI GAWTC 3 cut(s) 231, 333, 419
TscAI CASTG 2 cut(s) 48, 229
TseFI GTSAC 1 cut(s) 224
TseI GCWGC 1 cut(s) 95
Tsp45I GTSAC 1 cut(s) 224
TspDTI ATGAA 1 cut(s) 227
TspGWI ACGGA 1 cut(s) 345
TspRI CASTG 2 cut(s) 48, 229
XmaJI CCTAGG 1 cut(s) 137
XspI CTAG 3 cut(s) 21, 138, 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.