Rh6DG510800

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0004260
Physical Location & Seq
Reverse (-)
66425603 .. 66427489
1887 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG510800.1

Sequence Viewer

Length: 954 bp
ATGGCAGAGGCTATGGCGACTCGACTGGCGGCGGCACTTGCACTGACGGATGACGCGGTGGTGGAGTTTGGGGAAGCTGGAGCAGCGGCTGTTGCTGCGTCTGGCTTTTATCTGGTGGGTCGTATGTTGGCACCTAGGGCAGAGGATGCTGCTGGATTGCCCAAGGCGATGGCGGCGGTATGGGGCCTGCGTGATCGTCTCTCGATCACGAAGGTTGAAGGGGACCGCTTTCAGTTCCGCTTTGCGGTGAAGGAGGAAGGTCAGAGAGTTCTGCACGGGGGCCCGTGGCATTTCAAGAATCGGATGCTATTGCTGGAGGAGTATGATGGCTTTGGGGCGCCGGAGTTGGTTCCATTGAACTCGTTGGAGGCATGGGTCACGGTGCGACGTCTACCTCCACGTCTGCAGACGGAAGAGTCATTGATGCTGATTGGATCGTCAATGGGCCGGTTTCTGCGGGCAGAGAAGTTTGCACTGCAGCGGGGTTCTCCTGAGCAACGCTTCCGGGTCCTCTTGGACGTTCGCCGGCGTCTCTGGGGGCAGCGGACTTTTGGCTTTACAGAGACGTTTTTGATTAAGAAAAGTGGAGGTGGGCTGACCGGACCTACAGGGTTAGTAGCGGGAGGTCAGGTGACTCCGGCGGCGGCTAGGAAAGAGCCGTCTAAGGTGATCCTGTCAGAGACTCCAAGGTGCAGCGCTGGTGTTCCTGCGACACCTACACCAATCCTGTTAGGGCCGATGGCAACAACTGGAGTGCCAAATCTCATGGATATTGTTGGCCGGTCCATGGAGATGCTAAAGGGGATACCAAGATGGAATGCTCCATGTCCCATTCAATCTGGAGTCCCACTGAATATACCGGTGGCTCCCATAGCTATAGAAGCAGCACAAGTAAGTGGTGTGAAGCGCTCAGCTTTTTCTGTGCAAGCTCGTCCAGAAATCAAGTTATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

317

Amino Acids

34.14

Weight (kDa)

9.89

Isoelectric Point (pI)

48.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 37 - 109 5.5e-12 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 415
AatII GACGTC 1 cut(s) 391
AccB1I GGYRCC 2 cut(s) 130, 337
AccI GTMKAC 1 cut(s) 391
AccII CGCG 1 cut(s) 56
AclWI GGATC 2 cut(s) 442, 664
AcoI YGGCCR 1 cut(s) 778
AcyI GRCGYC 3 cut(s) 338, 388, 529
AfeI AGCGCT 2 cut(s) 697, 908
AfiI CCNNNNNNNGG 2 cut(s) 244, 787
AgeI ACCGGT 1 cut(s) 859
AgsI TTSAA 4 cut(s) 218, 295, 358, 836
AjiI CACGTC 1 cut(s) 401
AluBI AGCT 4 cut(s) 77, 875, 914, 929
AluI AGCT 4 cut(s) 77, 875, 914, 929
Alw26I GTCTC 4 cut(s) 203, 536, 557, 674
AlwI GGATC 2 cut(s) 442, 664
AlwNI CAGNNNCTG 1 cut(s) 89
Aor51HI AGCGCT 2 cut(s) 697, 908
AoxI GGCC 5 cut(s) 184, 280, 445, 734, 778
ApaI GGGCCC 1 cut(s) 284
ApeKI GCWGC 7 cut(s) 83, 95, 149, 478, 541, 693, 884
AsiGI ACCGGT 1 cut(s) 859
AspA2I CCTAGG 1 cut(s) 134
AspLEI GCGC 3 cut(s) 340, 698, 909
AspS9I GGNCC 9 cut(s) 184, 223, 280, 281, 445, 508, 602, 734, 783
AsuC2I CCSGG 1 cut(s) 506
AsuHPI GGTGA 3 cut(s) 259, 643, 679
AvaII GGWCC 4 cut(s) 223, 508, 602, 783
AvrII CCTAGG 1 cut(s) 134
BaeGI GKGCMC 1 cut(s) 284
BanI GGYRCC 2 cut(s) 130, 337
BanII GRGCYC 1 cut(s) 284
BbvI GCAGC 7 cut(s) 82, 95, 136, 490, 553, 705, 896
BccI CCATC 4 cut(s) 163, 320, 733, 807
BceAI ACGGC 1 cut(s) 643
BciVI GTATCC 1 cut(s) 798
BcnI CCSGG 1 cut(s) 506
BcoDI GTCTC 4 cut(s) 203, 536, 557, 674
BfaI CTAG 2 cut(s) 135, 648
BfmI CTRYAG 4 cut(s) 404, 476, 606, 876
BfoI RGCGCY 3 cut(s) 341, 699, 910
BfuI GTATCC 1 cut(s) 798
BlnI CCTAGG 1 cut(s) 134
BlpI GCTNAGC 1 cut(s) 910
Bme1390I CCNGG 1 cut(s) 506
Bme18I GGWCC 4 cut(s) 223, 508, 602, 783
BmgBI CACGTC 1 cut(s) 401
BmgT120I GGNCC 9 cut(s) 184, 223, 280, 281, 445, 508, 602, 734, 783
BmiI GGNNCC 9 cut(s) 132, 185, 224, 281, 282, 339, 351, 509, 867
BmrFI CCNGG 1 cut(s) 506
BmsI GCATC 4 cut(s) 136, 294, 414, 783
BpmI CTGGAG 4 cut(s) 99, 335, 771, 861
Bpu10I CCTNAGC 1 cut(s) 492
Bpu1102I GCTNAGC 1 cut(s) 910
BpuMI CCSGG 1 cut(s) 506
BsaHI GRCGYC 3 cut(s) 338, 388, 529
BsaJI CCNNGG 5 cut(s) 134, 162, 284, 686, 786
BsaWI WCCGGW 2 cut(s) 599, 859
BsaXI ACNNNNNCTCC 2 cut(s) 615, 645
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 787
Bse118I RCCGGY 4 cut(s) 447, 525, 780, 859
Bse1I ACTGG 2 cut(s) 30, 754
BseDI CCNNGG 5 cut(s) 134, 162, 284, 686, 786
BseGI GGATG 3 cut(s) 55, 151, 309
BseLI CCNNNNNNNGG 2 cut(s) 244, 787
BseMII CTCAG 2 cut(s) 483, 924
BseNI ACTGG 2 cut(s) 30, 754
BseRI GAGGAG 1 cut(s) 332
BseSI GKGCMC 1 cut(s) 284
BseXI GCAGC 7 cut(s) 82, 95, 136, 490, 553, 705, 896
BsgI GTGCAG 2 cut(s) 257, 712
Bsh1236I CGCG 1 cut(s) 56
BshFI GGCC 5 cut(s) 186, 282, 447, 736, 780
BshNI GGYRCC 2 cut(s) 130, 337
BshTI ACCGGT 1 cut(s) 859
BsiSI CCGG 8 cut(s) 341, 448, 505, 526, 600, 638, 781, 860
BslFI GGGAC 3 cut(s) 236, 813, 830
BslI CCNNNNNNNGG 2 cut(s) 244, 787
BsmAI GTCTC 4 cut(s) 203, 536, 557, 674
BsmBI CGTCTC 3 cut(s) 203, 536, 557
BsmFI GGGAC 3 cut(s) 236, 813, 830
BsmI GAATGC 1 cut(s) 823
BsnI GGCC 5 cut(s) 186, 282, 447, 736, 780
Bsp120I GGGCCC 1 cut(s) 280
Bsp1286I GDGCHC 1 cut(s) 284
Bsp143I GATC 4 cut(s) 193, 204, 434, 669
Bsp1720I GCTNAGC 1 cut(s) 910
Bsp19I CCATGG 1 cut(s) 786
BspANI GGCC 5 cut(s) 186, 282, 447, 736, 780
BspCNI CTCAG 2 cut(s) 484, 923
BspFNI CGCG 1 cut(s) 56
BspLI GGNNCC 9 cut(s) 132, 185, 224, 281, 282, 339, 351, 509, 867
BspMAI CTGCAG 2 cut(s) 408, 480
BspPI GGATC 2 cut(s) 442, 664
BspT107I GGYRCC 2 cut(s) 130, 337
BsrFI RCCGGY 4 cut(s) 447, 525, 780, 859
BsrI ACTGG 2 cut(s) 30, 754
BssAI RCCGGY 4 cut(s) 447, 525, 780, 859
BssECI CCNNGG 5 cut(s) 134, 162, 284, 686, 786
BssMI GATC 4 cut(s) 193, 204, 434, 669
BssNI GRCGYC 3 cut(s) 338, 388, 529
BssT1I CCWWGG 4 cut(s) 134, 162, 686, 786
Bst4CI ACNGT 1 cut(s) 382
Bst6I CTCTTC 1 cut(s) 408
BstACI GRCGYC 3 cut(s) 338, 388, 529
BstAPI GCANNNNNTGC 1 cut(s) 146
BstC8I GCNNGC 4 cut(s) 188, 459, 527, 927
BstDEI CTNAG 3 cut(s) 492, 663, 910
BstDSI CCRYGG 2 cut(s) 284, 786
BstF5I GGATG 3 cut(s) 55, 151, 309
BstFNI CGCG 1 cut(s) 56
BstH2I RGCGCY 3 cut(s) 341, 699, 910
BstHHI GCGC 3 cut(s) 340, 698, 909
BstKTI GATC 4 cut(s) 196, 207, 437, 672
BstMAI GTCTC 4 cut(s) 203, 536, 557, 674
BstMBI GATC 4 cut(s) 193, 204, 434, 669
BstMWI GCNNNNNNNGC 9 cut(s) 38, 83, 92, 95, 137, 146, 173, 872, 881
BstSCI CCNGG 1 cut(s) 504
BstSFI CTRYAG 4 cut(s) 404, 476, 606, 876
BstSLI GKGCMC 1 cut(s) 284
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 7 cut(s) 82, 95, 136, 490, 553, 705, 896
BstXI CCANNNNNNTGG 1 cut(s) 169
BsuI GTATCC 1 cut(s) 798
BsuRI GGCC 5 cut(s) 186, 282, 447, 736, 780
BtgI CCRYGG 2 cut(s) 284, 786
BtgZI GCGATG 1 cut(s) 182
BtrI CACGTC 1 cut(s) 401
BtsCI GGATG 3 cut(s) 55, 151, 309
BtsI GCAGTG 1 cut(s) 473
BtsIMutI CAGTG 3 cut(s) 41, 473, 848
Cac8I GCNNGC 4 cut(s) 188, 459, 527, 927
CaiI CAGNNNCTG 1 cut(s) 89
CfoI GCGC 3 cut(s) 340, 698, 909
Cfr10I RCCGGY 4 cut(s) 447, 525, 780, 859
Cfr13I GGNCC 9 cut(s) 184, 223, 280, 281, 445, 508, 602, 734, 783
CseI GACGC 3 cut(s) 62, 87, 518
CspAI ACCGGT 1 cut(s) 859
CviAII CATG 4 cut(s) 372, 766, 787, 825
DdeI CTNAG 3 cut(s) 492, 663, 910
DinI GGCGCC 1 cut(s) 339
DpnI GATC 4 cut(s) 195, 206, 436, 671
DpnII GATC 4 cut(s) 193, 204, 434, 669
DrdI GACNNNNNNGTC 1 cut(s) 415
DseDI GACNNNNNNGTC 1 cut(s) 415
EaeI YGGCCR 1 cut(s) 778
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
Eco130I CCWWGG 4 cut(s) 134, 162, 686, 786
Eco24I GRGCYC 1 cut(s) 284
Eco47I GGWCC 4 cut(s) 223, 508, 602, 783
Eco47III AGCGCT 2 cut(s) 697, 908
EcoO109I RGGNCCY 3 cut(s) 184, 280, 508
EcoT14I CCWWGG 4 cut(s) 134, 162, 686, 786
EcoT38I GRGCYC 1 cut(s) 284
EgeI GGCGCC 1 cut(s) 339
EheI GGCGCC 1 cut(s) 339
ErhI CCWWGG 4 cut(s) 134, 162, 686, 786
Esp3I CGTCTC 3 cut(s) 203, 536, 557
FaeI CATG 4 cut(s) 375, 769, 790, 828
FaqI GGGAC 3 cut(s) 236, 813, 830
FatI CATG 4 cut(s) 371, 765, 786, 824
FauI CCCGC 3 cut(s) 450, 474, 613
FblI GTMKAC 1 cut(s) 391
FokI GGATG 3 cut(s) 62, 158, 316
FriOI GRGCYC 1 cut(s) 284
FspBI CTAG 2 cut(s) 135, 648
GlaI GCGC 3 cut(s) 339, 697, 908
GsuI CTGGAG 4 cut(s) 99, 335, 771, 861
HaeII RGCGCY 3 cut(s) 341, 699, 910
HaeIII GGCC 5 cut(s) 186, 282, 447, 736, 780
HapII CCGG 8 cut(s) 341, 448, 505, 526, 600, 638, 781, 860
HgaI GACGC 3 cut(s) 62, 87, 518
HhaI GCGC 3 cut(s) 340, 698, 909
Hin1I GRCGYC 3 cut(s) 338, 388, 529
Hin1II CATG 4 cut(s) 375, 769, 790, 828
Hin6I GCGC 3 cut(s) 338, 696, 907
HinP1I GCGC 3 cut(s) 338, 696, 907
HinfI GANTC 6 cut(s) 19, 298, 416, 634, 682, 843
HpaII CCGG 8 cut(s) 341, 448, 505, 526, 600, 638, 781, 860
HphI GGTGA 3 cut(s) 259, 643, 679
Hpy166II GTNNAC 1 cut(s) 392
Hpy188I TCNGA 3 cut(s) 264, 303, 679
Hpy188III TCNNGA 6 cut(s) 202, 208, 295, 491, 840, 935
Hpy8I GTNNAC 1 cut(s) 392
Hpy99I CGWCG 1 cut(s) 390
HpyAV CCTTC 4 cut(s) 205, 212, 244, 251
HpyCH4III ACNGT 1 cut(s) 382
HpyCH4IV ACGT 4 cut(s) 388, 400, 519, 566
HpyCH4V TGCA 7 cut(s) 41, 274, 406, 473, 478, 693, 925
HpyF10VI GCNNNNNNNGC 9 cut(s) 38, 83, 92, 95, 137, 146, 173, 872, 881
HpyF3I CTNAG 3 cut(s) 492, 663, 910
HpySE526I ACGT 4 cut(s) 388, 400, 519, 566
Hsp92I GRCGYC 3 cut(s) 338, 388, 529
Hsp92II CATG 4 cut(s) 375, 769, 790, 828
HspAI GCGC 3 cut(s) 338, 696, 907
KasI GGCGCC 1 cut(s) 337
KroI GCCGGC 1 cut(s) 525
KroNI GCCGGC 1 cut(s) 527
Kzo9I GATC 4 cut(s) 193, 204, 434, 669
LmnI GCTCC 3 cut(s) 80, 826, 871
Lsp1109I GCAGC 7 cut(s) 82, 95, 136, 490, 553, 705, 896
LweI GCATC 4 cut(s) 136, 294, 414, 783
MaeI CTAG 2 cut(s) 135, 648
MaeII ACGT 4 cut(s) 388, 400, 519, 566
MaeIII GTNAC 2 cut(s) 376, 631
MalI GATC 4 cut(s) 195, 206, 436, 671
MboI GATC 4 cut(s) 193, 204, 434, 669
MboII GAAGA 1 cut(s) 425
MhlI GDGCHC 1 cut(s) 284
Mly113I GGCGCC 1 cut(s) 338
MlyI GAGTC 5 cut(s) 13, 425, 628, 676, 852
MmeI TCCRAC 1 cut(s) 345
MnlI CCTC 8 cut(s) 136, 247, 310, 361, 405, 521, 581, 617
MreI CGCCGGCG 1 cut(s) 525
MroNI GCCGGC 1 cut(s) 525
MseI TTAA 2 cut(s) 576, 952
MslI CAYNNNNRTG 1 cut(s) 791
MspA1I CMGCKG 3 cut(s) 86, 481, 544
MspI CCGG 8 cut(s) 341, 448, 505, 526, 600, 638, 781, 860
MspR9I CCNGG 1 cut(s) 506
Mva1269I GAATGC 1 cut(s) 823
MvnI CGCG 1 cut(s) 56
MwoI GCNNNNNNNGC 9 cut(s) 38, 83, 92, 95, 137, 146, 173, 872, 881
NaeI GCCGGC 1 cut(s) 527
NarI GGCGCC 1 cut(s) 338
NciI CCSGG 1 cut(s) 506
NcoI CCATGG 1 cut(s) 786
NdeII GATC 4 cut(s) 193, 204, 434, 669
NgoMIV GCCGGC 1 cut(s) 525
NlaIII CATG 4 cut(s) 375, 769, 790, 828
NlaIV GGNNCC 9 cut(s) 132, 185, 224, 281, 282, 339, 351, 509, 867
NmuCI GTSAC 2 cut(s) 376, 631
PctI GAATGC 1 cut(s) 823
PdiI GCCGGC 1 cut(s) 527
PfeI GAWTC 1 cut(s) 298
PinAI ACCGGT 1 cut(s) 859
PleI GAGTC 5 cut(s) 13, 424, 628, 676, 851
PluTI GGCGCC 1 cut(s) 341
PpsI GAGTC 5 cut(s) 13, 424, 628, 676, 851
PpuMI RGGWCCY 1 cut(s) 508
Psp5II RGGWCCY 1 cut(s) 508
PspN4I GGNNCC 9 cut(s) 132, 185, 224, 281, 282, 339, 351, 509, 867
PspOMI GGGCCC 1 cut(s) 280
PspPI GGNCC 9 cut(s) 184, 223, 280, 281, 445, 508, 602, 734, 783
PspPPI RGGWCCY 1 cut(s) 508
PstI CTGCAG 2 cut(s) 408, 480
PstNI CAGNNNCTG 1 cut(s) 89
RseI CAYNNNNRTG 1 cut(s) 791
SaqAI TTAA 2 cut(s) 576, 952
Sau3AI GATC 4 cut(s) 193, 204, 434, 669
Sau96I GGNCC 9 cut(s) 184, 223, 280, 281, 445, 508, 602, 734, 783
SchI GAGTC 5 cut(s) 13, 425, 628, 676, 852
ScrFI CCNGG 1 cut(s) 506
SduI GDGCHC 1 cut(s) 284
SfaNI GCATC 4 cut(s) 136, 294, 414, 783
SfcI CTRYAG 4 cut(s) 404, 476, 606, 876
SfoI GGCGCC 1 cut(s) 339
SgrAI CRCCGGYG 1 cut(s) 525
SinI GGWCC 4 cut(s) 223, 508, 602, 783
SmiMI CAYNNNNRTG 1 cut(s) 791
SspDI GGCGCC 1 cut(s) 337
SspMI CTAG 2 cut(s) 135, 648
StyD4I CCNGG 1 cut(s) 504
StyI CCWWGG 4 cut(s) 134, 162, 686, 786
TaaI ACNGT 1 cut(s) 382
TaiI ACGT 4 cut(s) 391, 403, 522, 569
TaqI TCGA 2 cut(s) 22, 203
TauI GCSGC 6 cut(s) 32, 35, 89, 176, 644, 647
TfiI GAWTC 1 cut(s) 298
Tru1I TTAA 2 cut(s) 576, 952
Tru9I TTAA 2 cut(s) 576, 952
TscAI CASTG 3 cut(s) 48, 480, 855
TseFI GTSAC 2 cut(s) 376, 631
TseI GCWGC 7 cut(s) 83, 95, 149, 478, 541, 693, 884
Tsp45I GTSAC 2 cut(s) 376, 631
TspGWI ACGGA 2 cut(s) 62, 425
TspRI CASTG 3 cut(s) 48, 480, 855
VpaK11BI GGWCC 4 cut(s) 223, 508, 602, 783
XmaJI CCTAGG 1 cut(s) 134
XmiI GTMKAC 1 cut(s) 391
XspI CTAG 2 cut(s) 135, 648
ZraI GACGTC 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.