Rh5BG212800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0004260
Physical Location & Seq
Forward (+)
24649930 .. 24650497
568 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG212800.1

Sequence Viewer

Length: 477 bp
ATGGGACGATTTTTGCGAGCAGACAAGTTTCCTGTGCAACGGCGATCACCAGAACAACGTTTCTTGGTCCTCCTGGACGTTCGCTGGTGGCTGTGGGGGCAGCGGACCTTCAACTTTACGAAGATGGCCAAGGCGGAGGTGGAATTGCACTACGAAAAGTTTCTGCTCAAGAAATTTGGTGTTGGGGTGATGGCAGGAATGCTGGGGATGGAGATGGGGGGTCCAGTAGCTCAGGTGGTTCTGCCAGAGACCCCTAGAGGTAGTTCTGGTGCTGCTGCGACACTAACACCACTAGTGATAGGGCTGACATCAACTGACGTCTTTGGAGATGTTGAAGGGTATACCAAGCAGGAATGCTCCTTCTCCCATTTAATCTGGAGTTCCACTAGATCTGCCGGTGGCACCTATGGCTATACAAGCGGCACAAGTGAGTGGGGCCAAGCGTTCACCATCTGTTGTGCAAGCTTGTCCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

17.47

Weight (kDa)

9.12

Isoelectric Point (pI)

44.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 321
AccB1I GGYRCC 1 cut(s) 401
AccI GTMKAC 1 cut(s) 341
AciI CCGC 3 cut(s) 103, 134, 420
AclI AACGTT 1 cut(s) 58
AcoI YGGCCR 1 cut(s) 126
AcsI RAATTY 1 cut(s) 173
AcyI GRCGYC 1 cut(s) 318
AgsI TTSAA 2 cut(s) 112, 335
AhlI ACTAGT 1 cut(s) 292
AjnI CCWGG 2 cut(s) 72, 470
AluBI AGCT 2 cut(s) 230, 465
AluI AGCT 2 cut(s) 230, 465
Alw26I GTCTC 1 cut(s) 242
AoxI GGCC 2 cut(s) 126, 436
ApeKI GCWGC 3 cut(s) 100, 272, 275
ApoI RAATTY 1 cut(s) 173
Asp700I GAANNNNTTC 1 cut(s) 159
AspS9I GGNCC 4 cut(s) 67, 105, 221, 436
AsuHPI GGTGA 3 cut(s) 39, 199, 439
AvaII GGWCC 3 cut(s) 67, 105, 221
BalI TGGCCA 1 cut(s) 128
BanI GGYRCC 1 cut(s) 401
BbvI GCAGC 3 cut(s) 112, 259, 262
BccI CCATC 5 cut(s) 118, 184, 202, 208, 458
BceAI ACGGC 1 cut(s) 56
BciT130I CCWGG 2 cut(s) 74, 472
BcoDI GTCTC 1 cut(s) 242
BcuI ACTAGT 1 cut(s) 292
BfaI CTAG 3 cut(s) 255, 293, 387
BglII AGATCT 1 cut(s) 389
BisI GCNGC 4 cut(s) 101, 273, 276, 421
BlsI GCNGC 4 cut(s) 102, 274, 277, 422
Bme1390I CCNGG 2 cut(s) 74, 472
Bme18I GGWCC 3 cut(s) 67, 105, 221
BmgT120I GGNCC 4 cut(s) 67, 105, 221, 436
BmiI GGNNCC 3 cut(s) 222, 403, 437
BmrFI CCNGG 2 cut(s) 74, 472
BpmI CTGGAG 1 cut(s) 397
Bpu10I CCTNAGC 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 152
BsaHI GRCGYC 1 cut(s) 318
BsaI GGTCTC 1 cut(s) 242
BsaJI CCNNGG 1 cut(s) 129
Bse118I RCCGGY 1 cut(s) 395
Bse1I ACTGG 1 cut(s) 224
BseBI CCWGG 2 cut(s) 74, 472
BseDI CCNNGG 1 cut(s) 129
BseGI GGATG 1 cut(s) 213
BseMII CTCAG 1 cut(s) 245
BseNI ACTGG 1 cut(s) 224
BseXI GCAGC 3 cut(s) 112, 259, 262
BseYI CCCAGC 1 cut(s) 202
BshFI GGCC 2 cut(s) 128, 438
BshNI GGYRCC 1 cut(s) 401
BsiSI CCGG 1 cut(s) 396
BslFI GGGAC 1 cut(s) 18
BsmAI GTCTC 1 cut(s) 242
BsmFI GGGAC 1 cut(s) 18
BsmI GAATGC 2 cut(s) 204, 359
BsnI GGCC 2 cut(s) 128, 438
Bso31I GGTCTC 1 cut(s) 242
Bsp143I GATC 2 cut(s) 44, 389
BspACI CCGC 3 cut(s) 103, 134, 420
BspANI GGCC 2 cut(s) 128, 438
BspCNI CTCAG 1 cut(s) 244
BspLI GGNNCC 3 cut(s) 222, 403, 437
BspT107I GGYRCC 1 cut(s) 401
BspTNI GGTCTC 1 cut(s) 242
BsrFI RCCGGY 1 cut(s) 395
BsrI ACTGG 1 cut(s) 224
BssAI RCCGGY 1 cut(s) 395
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 2 cut(s) 44, 389
BssNAI GTATAC 1 cut(s) 342
BssNI GRCGYC 1 cut(s) 318
BssT1I CCWWGG 1 cut(s) 129
Bst1107I GTATAC 1 cut(s) 342
Bst2UI CCWGG 2 cut(s) 74, 472
BstACI GRCGYC 1 cut(s) 318
BstC8I GCNNGC 2 cut(s) 18, 463
BstDEI CTNAG 1 cut(s) 231
BstF5I GGATG 1 cut(s) 213
BstKTI GATC 2 cut(s) 47, 392
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 2 cut(s) 44, 389
BstMWI GCNNNNNNNGC 3 cut(s) 97, 408, 417
BstNI CCWGG 2 cut(s) 74, 472
BstSCI CCNGG 2 cut(s) 72, 470
BstV1I GCAGC 3 cut(s) 112, 259, 262
BstX2I RGATCY 1 cut(s) 389
BstYI RGATCY 1 cut(s) 389
BstZ17I GTATAC 1 cut(s) 342
BsuRI GGCC 2 cut(s) 128, 438
BtsCI GGATG 1 cut(s) 213
Cac8I GCNNGC 2 cut(s) 18, 463
Cfr10I RCCGGY 1 cut(s) 395
Cfr13I GGNCC 4 cut(s) 67, 105, 221, 436
CviJI RGCY 7 cut(s) 91, 128, 230, 304, 411, 438, 465
CviKI_1 RGCY 7 cut(s) 91, 128, 230, 304, 411, 438, 465
DdeI CTNAG 1 cut(s) 231
DpnI GATC 2 cut(s) 46, 391
DpnII GATC 2 cut(s) 44, 389
EaeI YGGCCR 1 cut(s) 126
EciI GGCGGA 1 cut(s) 149
Eco130I CCWWGG 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 242
Eco47I GGWCC 3 cut(s) 67, 105, 221
EcoRII CCWGG 2 cut(s) 72, 470
EcoT14I CCWWGG 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 129
FaiI YATR 3 cut(s) 342, 408, 414
FaqI GGGAC 1 cut(s) 18
FblI GTMKAC 1 cut(s) 341
Fnu4HI GCNGC 4 cut(s) 101, 273, 276, 421
FokI GGATG 1 cut(s) 220
Fsp4HI GCNGC 4 cut(s) 101, 273, 276, 421
FspBI CTAG 3 cut(s) 255, 293, 387
GluI GCNGC 4 cut(s) 101, 273, 276, 421
GsaI CCCAGC 1 cut(s) 206
GsuI CTGGAG 1 cut(s) 397
HaeIII GGCC 2 cut(s) 128, 438
HapII CCGG 1 cut(s) 396
Hin1I GRCGYC 1 cut(s) 318
HindIII AAGCTT 1 cut(s) 463
HpaII CCGG 1 cut(s) 396
HphI GGTGA 3 cut(s) 39, 199, 439
Hpy166II GTNNAC 2 cut(s) 342, 447
Hpy188III TCNNGA 2 cut(s) 169, 376
Hpy8I GTNNAC 2 cut(s) 342, 447
HpyAV CCTTC 3 cut(s) 118, 329, 370
HpyCH4IV ACGT 3 cut(s) 58, 78, 318
HpyCH4V TGCA 3 cut(s) 37, 148, 461
HpyF10VI GCNNNNNNNGC 3 cut(s) 97, 408, 417
HpyF3I CTNAG 1 cut(s) 231
HpySE526I ACGT 3 cut(s) 58, 78, 318
Hsp92I GRCGYC 1 cut(s) 318
Kzo9I GATC 2 cut(s) 44, 389
LmnI GCTCC 1 cut(s) 362
Lsp1109I GCAGC 3 cut(s) 112, 259, 262
MaeI CTAG 3 cut(s) 255, 293, 387
MaeII ACGT 3 cut(s) 58, 78, 318
MalI GATC 2 cut(s) 46, 391
MboI GATC 2 cut(s) 44, 389
MboII GAAGA 1 cut(s) 133
MflI RGATCY 1 cut(s) 389
MlsI TGGCCA 1 cut(s) 128
MluCI AATT 2 cut(s) 143, 173
MluNI TGGCCA 1 cut(s) 128
MnlI CCTC 3 cut(s) 80, 130, 251
Mox20I TGGCCA 1 cut(s) 128
MroXI GAANNNNTTC 1 cut(s) 159
MscI TGGCCA 1 cut(s) 128
MseI TTAA 1 cut(s) 371
Msp20I TGGCCA 1 cut(s) 128
MspA1I CMGCKG 1 cut(s) 103
MspI CCGG 1 cut(s) 396
MspR9I CCNGG 2 cut(s) 74, 472
Mva1269I GAATGC 2 cut(s) 204, 359
MvaI CCWGG 2 cut(s) 74, 472
MwoI GCNNNNNNNGC 3 cut(s) 97, 408, 417
NdeII GATC 2 cut(s) 44, 389
NlaIV GGNNCC 3 cut(s) 222, 403, 437
PcsI WCGNNNNNNNCGW 1 cut(s) 13
PctI GAATGC 2 cut(s) 204, 359
PdmI GAANNNNTTC 1 cut(s) 159
PfoI TCCNGGA 1 cut(s) 72
PkrI GCNGC 4 cut(s) 102, 274, 277, 422
Psp1406I AACGTT 1 cut(s) 58
Psp6I CCWGG 2 cut(s) 72, 470
PspFI CCCAGC 1 cut(s) 202
PspGI CCWGG 2 cut(s) 72, 470
PspN4I GGNNCC 3 cut(s) 222, 403, 437
PspPI GGNCC 4 cut(s) 67, 105, 221, 436
PsuI RGATCY 1 cut(s) 389
SaqAI TTAA 1 cut(s) 371
SatI GCNGC 4 cut(s) 101, 273, 276, 421
Sau3AI GATC 2 cut(s) 44, 389
Sau96I GGNCC 4 cut(s) 67, 105, 221, 436
ScrFI CCNGG 2 cut(s) 74, 472
SinI GGWCC 3 cut(s) 67, 105, 221
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
SpeI ACTAGT 1 cut(s) 292
Sse9I AATT 2 cut(s) 143, 173
SsiI CCGC 3 cut(s) 103, 134, 420
SspMI CTAG 3 cut(s) 255, 293, 387
StyD4I CCNGG 2 cut(s) 72, 470
StyI CCWWGG 1 cut(s) 129
TaiI ACGT 3 cut(s) 61, 81, 321
TasI AATT 2 cut(s) 143, 173
TauI GCSGC 1 cut(s) 423
Tru1I TTAA 1 cut(s) 371
Tru9I TTAA 1 cut(s) 371
TseI GCWGC 3 cut(s) 100, 272, 275
VpaK11BI GGWCC 3 cut(s) 67, 105, 221
XapI RAATTY 1 cut(s) 173
XcmI CCANNNNNNNNNTGG 1 cut(s) 136
XmiI GTMKAC 1 cut(s) 341
XmnI GAANNNNTTC 1 cut(s) 159
XspI CTAG 3 cut(s) 255, 293, 387
ZraI GACGTC 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.