Rmu_sc0001738.1_g000040

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001738.1
OG0001584
Physical Location & Seq
Forward (+)
185270 .. 189943
4674 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1377 bp
atgtcctatttctacagaatcttctgggagtccaagaaatttgaaggtgatacaaacatgtttgagccctggtatggaactgcttactgcatggagaaaattacgggctccagaattcaggaatggatagcatcttccccaaatgagaatttacacctaccagcacgtaatgttttcatccccacggacttgtctcttaagaatgagaatgagaaggtcaaatgtccagttctgttaagaaagtcaccatatgcaacactatggtacaagcctgatacaatgttttttactcctaaagcatacgtcaagattgatttcaattgtccccatgcaagtggctcccctgaagcagaagtcctgactaatatttttactcacttgttaatggattacttgaatgactatgcttattatgctaaggttgctggactgtactatggaataaaccacacagaaagtggttttcaggtgactctggttggttataatcacaaattgaggattttactggaaacggtagttgagaagatggcaagctttaaagtgaaagctgacaggttctctgtgatcaaggaaatggtcacaaagaagtaccaaaattacaaatttcaacagccctatgagcaggctatgtactatttctcattaattctacagtaccagacatggccattgatggaacaactagaagttcttcctcatcttgaagttgaagatcttgctaagtttgttccattgatgctctcaagggccttcttagagtgttacacagcagagagattttgtttttcaggaaaccttgaaagtactgaagctgagtcaatgatcctgcacgtagaagatgttttttttaagggttcaaaccctatttgccaacctttattcccatcccaacatttgacaaatagagtcgtgaagcttgaaaagggaaaaaactttatctatcctgtggaaggccttaatccaagtgatgagaattcttcccttgtccactatatccagagcaatgtaaatactttgatcgatatgaagcttgagaagcacaagaacttgagggaagaagctggattttattggagggagatttctgatgggaccctcaagtttgatcgtaaagaagcagagattgcagcactgaggcagcttacacaacaagagttgattgagttttataatgagcatataaaagttggggcacctcgtaagaggacattgagtgtacgagtatatgggaatcctcattcatctgagtatgcatcagataaaagctccccagtgcaaccttgcactgtcaaaatcgatgatatattcactttcagaaggtcacagcctctttatggttcattcaagggaaactttggtcatgtgaagttgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

458

Amino Acids

53.4

Weight (kDa)

6.9

Isoelectric Point (pI)

42.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16_M PF16187 6 - 230 5.4e-82 Middle or third domain of peptidase_M16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 486, 1173
AccB1I GGYRCC 1 cut(s) 1195
AclWI GGATC 1 cut(s) 820
AcoI YGGCCR 1 cut(s) 668
AcsI RAATTY 5 cut(s) 38, 114, 148, 605, 976
AcuI CTGAAG 2 cut(s) 366, 831
AfaI GTAC 7 cut(s) 266, 434, 593, 635, 659, 808, 1221
AfiI CCNNNNNNNGG 3 cut(s) 74, 953, 1337
AflII CTTAAG 1 cut(s) 197
AflIII ACRYGT 1 cut(s) 57
AjnI CCWGG 1 cut(s) 68
AluBI AGCT 8 cut(s) 537, 551, 815, 919, 1033, 1064, 1144, 1269
AluI AGCT 8 cut(s) 537, 551, 815, 919, 1033, 1064, 1144, 1269
Alw26I GTCTC 1 cut(s) 198
AlwI GGATC 1 cut(s) 820
AoxI GGCC 3 cut(s) 668, 750, 955
ApeKI GCWGC 2 cut(s) 1130, 1141
ApoI RAATTY 5 cut(s) 38, 114, 148, 605, 976
AseI ATTAAT 1 cut(s) 647
Asp700I GAANNNNTTC 1 cut(s) 693
AspS9I GGNCC 2 cut(s) 750, 1095
AsuHPI GGTGA 3 cut(s) 59, 237, 481
AvaII GGWCC 1 cut(s) 1095
BaeGI GKGCMC 1 cut(s) 1198
BalI TGGCCA 1 cut(s) 670
BanI GGYRCC 1 cut(s) 1195
BanII GRGCYC 2 cut(s) 69, 110
BbvI GCAGC 2 cut(s) 1142, 1153
BccI CCATC 4 cut(s) 523, 670, 895, 1085
BciT130I CCWGG 1 cut(s) 70
BclI TGATCA 1 cut(s) 567
BcoDI GTCTC 1 cut(s) 198
BfaI CTAG 1 cut(s) 686
BfmI CTRYAG 2 cut(s) 13, 653
BfrI CTTAAG 1 cut(s) 197
BglII AGATCT 1 cut(s) 715
BisI GCNGC 2 cut(s) 1131, 1142
BlsI GCNGC 2 cut(s) 1132, 1143
BmcAI AGTACT 1 cut(s) 808
Bme1390I CCNGG 1 cut(s) 70
Bme18I GGWCC 1 cut(s) 1095
BmgT120I GGNCC 2 cut(s) 750, 1095
BmiI GGNNCC 5 cut(s) 109, 340, 1096, 1097, 1197
BmrFI CCNGG 1 cut(s) 70
BmrI ACTGGG 1 cut(s) 1268
BmsI GCATC 3 cut(s) 140, 729, 1265
BmuI ACTGGG 1 cut(s) 1268
BpmI CTGGAG 1 cut(s) 94
Bpu10I CCTNAGC 1 cut(s) 417
BpuEI CTTGAG 4 cut(s) 730, 1055, 1072, 1085
Bsa29I ATCGAT 2 cut(s) 1023, 1299
BsaAI YACGTR 2 cut(s) 167, 835
BsaJI CCNNGG 2 cut(s) 68, 183
Bsc4I CCNNNNNNNGG 3 cut(s) 74, 953, 1337
Bse1I ACTGG 3 cut(s) 227, 513, 1274
Bse3DI GCAATG 1 cut(s) 1012
BseBI CCWGG 1 cut(s) 70
BseCI ATCGAT 2 cut(s) 1023, 1299
BseDI CCNNGG 2 cut(s) 68, 183
BseGI GGATG 2 cut(s) 177, 887
BseLI CCNNNNNNNGG 3 cut(s) 74, 953, 1337
BseMI GCAATG 1 cut(s) 1012
BseMII CTCAG 3 cut(s) 807, 1127, 1239
BseNI ACTGG 3 cut(s) 227, 513, 1274
BseSI GKGCMC 1 cut(s) 1198
BseXI GCAGC 2 cut(s) 1142, 1153
BsgI GTGCAG 1 cut(s) 815
BshFI GGCC 3 cut(s) 670, 752, 957
BshNI GGYRCC 1 cut(s) 1195
BshVI ATCGAT 2 cut(s) 1023, 1299
BslFI GGGAC 2 cut(s) 309, 1108
BslI CCNNNNNNNGG 3 cut(s) 74, 953, 1337
BsmAI GTCTC 1 cut(s) 198
BsmFI GGGAC 2 cut(s) 309, 1108
BsnI GGCC 3 cut(s) 670, 752, 957
Bsp1286I GDGCHC 3 cut(s) 69, 110, 1198
Bsp143I GATC 5 cut(s) 567, 715, 825, 1020, 1108
BspANI GGCC 3 cut(s) 670, 752, 957
BspCNI CTCAG 3 cut(s) 808, 1128, 1240
BspDI ATCGAT 2 cut(s) 1023, 1299
BspLI GGNNCC 5 cut(s) 109, 340, 1096, 1097, 1197
BspPI GGATC 1 cut(s) 820
BspT107I GGYRCC 1 cut(s) 1195
BspTI CTTAAG 1 cut(s) 197
BsrDI GCAATG 1 cut(s) 1012
BsrI ACTGG 3 cut(s) 227, 513, 1274
BssECI CCNNGG 2 cut(s) 68, 183
BssMI GATC 5 cut(s) 567, 715, 825, 1020, 1108
Bst2UI CCWGG 1 cut(s) 70
Bst4CI ACNGT 4 cut(s) 432, 517, 657, 1291
BstAFI CTTAAG 1 cut(s) 197
BstAPI GCANNNNNTGC 1 cut(s) 1127
BstBAI YACGTR 2 cut(s) 167, 835
BstC8I GCNNGC 2 cut(s) 535, 627
BstDEI CTNAG 6 cut(s) 417, 723, 757, 816, 1136, 1248
BstDSI CCRYGG 1 cut(s) 183
BstENI CCTNNNNNAGG 1 cut(s) 951
BstF5I GGATG 2 cut(s) 177, 887
BstKTI GATC 5 cut(s) 570, 718, 828, 1023, 1111
BstMAI GTCTC 1 cut(s) 198
BstMBI GATC 5 cut(s) 567, 715, 825, 1020, 1108
BstMWI GCNNNNNNNGC 5 cut(s) 413, 422, 622, 1039, 1127
BstNI CCWGG 1 cut(s) 70
BstNSI RCATGY 1 cut(s) 61
BstSCI CCNGG 1 cut(s) 68
BstSFI CTRYAG 2 cut(s) 13, 653
BstSLI GKGCMC 1 cut(s) 1198
BstV1I GCAGC 2 cut(s) 1142, 1153
BstX2I RGATCY 1 cut(s) 715
BstXI CCANNNNNNTGG 1 cut(s) 335
BstYI RGATCY 1 cut(s) 715
Bsu15I ATCGAT 2 cut(s) 1023, 1299
BsuRI GGCC 3 cut(s) 670, 752, 957
BsuTUI ATCGAT 2 cut(s) 1023, 1299
BtgI CCRYGG 1 cut(s) 183
BtsCI GGATG 2 cut(s) 177, 887
BtsIMutI CAGTG 3 cut(s) 1133, 1281, 1287
Cac8I GCNNGC 2 cut(s) 535, 627
Cfr13I GGNCC 2 cut(s) 750, 1095
ClaI ATCGAT 2 cut(s) 1023, 1299
Csp6I GTAC 7 cut(s) 265, 433, 592, 634, 658, 807, 1220
CviAII CATG 5 cut(s) 58, 91, 329, 666, 1364
CviQI GTAC 7 cut(s) 265, 433, 592, 634, 658, 807, 1220
DdeI CTNAG 6 cut(s) 417, 723, 757, 816, 1136, 1248
DpnI GATC 5 cut(s) 569, 717, 827, 1022, 1110
DpnII GATC 5 cut(s) 567, 715, 825, 1020, 1108
DraI TTTAAA 1 cut(s) 541
EaeI YGGCCR 1 cut(s) 668
Eco147I AGGCCT 1 cut(s) 957
Eco24I GRGCYC 2 cut(s) 69, 110
Eco47I GGWCC 1 cut(s) 1095
Eco57I CTGAAG 2 cut(s) 366, 831
EcoNI CCTNNNNNAGG 1 cut(s) 951
EcoO109I RGGNCCY 2 cut(s) 750, 1095
EcoRI GAATTC 2 cut(s) 114, 976
EcoRII CCWGG 1 cut(s) 68
EcoT22I ATGCAT 1 cut(s) 1258
EcoT38I GRGCYC 2 cut(s) 69, 110
FaeI CATG 5 cut(s) 61, 94, 332, 669, 1367
FalI AAGNNNNNCTT 2 cut(s) 1340, 1372
FaqI GGGAC 2 cut(s) 309, 1108
FatI CATG 5 cut(s) 57, 90, 328, 665, 1363
FauNDI CATATG 1 cut(s) 250
FbaI TGATCA 1 cut(s) 567
Fnu4HI GCNGC 2 cut(s) 1131, 1142
FokI GGATG 2 cut(s) 164, 874
FriOI GRGCYC 2 cut(s) 69, 110
Fsp4HI GCNGC 2 cut(s) 1131, 1142
FspBI CTAG 1 cut(s) 686
GluI GCNGC 2 cut(s) 1131, 1142
GsuI CTGGAG 1 cut(s) 94
HaeIII GGCC 3 cut(s) 670, 752, 957
Hin1II CATG 5 cut(s) 61, 94, 332, 669, 1367
HindIII AAGCTT 3 cut(s) 535, 917, 1031
HinfI GANTC 6 cut(s) 18, 29, 472, 818, 909, 1234
HphI GGTGA 3 cut(s) 59, 237, 481
Hpy166II GTNNAC 2 cut(s) 991, 1220
Hpy188I TCNGA 4 cut(s) 1090, 1249, 1261, 1319
Hpy188III TCNNGA 8 cut(s) 111, 119, 307, 358, 704, 792, 913, 1000
Hpy8I GTNNAC 2 cut(s) 991, 1220
HpyAV CCTTC 5 cut(s) 38, 208, 763, 947, 1314
HpyCH4III ACNGT 4 cut(s) 432, 517, 657, 1291
HpyCH4IV ACGT 3 cut(s) 166, 303, 834
HpyCH4V TGCA 8 cut(s) 90, 254, 332, 832, 1130, 1256, 1279, 1287
HpyF10VI GCNNNNNNNGC 5 cut(s) 413, 422, 622, 1039, 1127
HpyF3I CTNAG 6 cut(s) 417, 723, 757, 816, 1136, 1248
HpySE526I ACGT 3 cut(s) 166, 303, 834
Hsp92II CATG 5 cut(s) 61, 94, 332, 669, 1367
KflI GGGWCCC 1 cut(s) 1095
Ksp22I TGATCA 1 cut(s) 567
Kzo9I GATC 5 cut(s) 567, 715, 825, 1020, 1108
LmnI GCTCC 3 cut(s) 113, 344, 1274
Lsp1109I GCAGC 2 cut(s) 1142, 1153
LweI GCATC 3 cut(s) 140, 729, 1265
MaeI CTAG 1 cut(s) 686
MaeII ACGT 3 cut(s) 166, 303, 834
MaeIII GTNAC 5 cut(s) 243, 469, 580, 764, 1323
MalI GATC 5 cut(s) 569, 717, 827, 1022, 1110
MboI GATC 5 cut(s) 567, 715, 825, 1020, 1108
MboII GAAGA 8 cut(s) 13, 126, 538, 686, 725, 851, 972, 1070
MfeI CAATTG 1 cut(s) 319
MflI RGATCY 1 cut(s) 715
MhlI GDGCHC 3 cut(s) 69, 110, 1198
MlsI TGGCCA 1 cut(s) 670
MluNI TGGCCA 1 cut(s) 670
MlyI GAGTC 4 cut(s) 38, 466, 827, 918
Mox20I TGGCCA 1 cut(s) 670
Mph1103I ATGCAT 1 cut(s) 1258
MroXI GAANNNNTTC 1 cut(s) 693
MscI TGGCCA 1 cut(s) 670
MseI TTAA 7 cut(s) 198, 236, 383, 540, 647, 852, 960
MslI CAYNNNNRTG 1 cut(s) 333
Msp20I TGGCCA 1 cut(s) 670
MspCI CTTAAG 1 cut(s) 197
MspR9I CCNGG 1 cut(s) 70
MunI CAATTG 1 cut(s) 319
MvaI CCWGG 1 cut(s) 70
MwoI GCNNNNNNNGC 5 cut(s) 413, 422, 622, 1039, 1127
NdeI CATATG 1 cut(s) 250
NdeII GATC 5 cut(s) 567, 715, 825, 1020, 1108
NlaIII CATG 5 cut(s) 61, 94, 332, 669, 1367
NlaIV GGNNCC 5 cut(s) 109, 340, 1096, 1097, 1197
NmuCI GTSAC 4 cut(s) 243, 469, 580, 1323
NsiI ATGCAT 1 cut(s) 1258
NspI RCATGY 1 cut(s) 61
PceI AGGCCT 1 cut(s) 957
PciI ACATGT 1 cut(s) 57
PdmI GAANNNNTTC 1 cut(s) 693
PfeI GAWTC 2 cut(s) 18, 1234
PkrI GCNGC 2 cut(s) 1132, 1143
PleI GAGTC 4 cut(s) 37, 466, 826, 917
PpsI GAGTC 4 cut(s) 37, 466, 826, 917
Ppu21I YACGTR 2 cut(s) 167, 835
PpuMI RGGWCCY 1 cut(s) 1095
PscI ACATGT 1 cut(s) 57
PshBI ATTAAT 1 cut(s) 647
PsiI TTATAA 2 cut(s) 486, 1173
Psp5II RGGWCCY 1 cut(s) 1095
Psp6I CCWGG 1 cut(s) 68
PspGI CCWGG 1 cut(s) 68
PspN4I GGNNCC 5 cut(s) 109, 340, 1096, 1097, 1197
PspPI GGNCC 2 cut(s) 750, 1095
PspPPI RGGWCCY 1 cut(s) 1095
PsuI RGATCY 1 cut(s) 715
RsaI GTAC 7 cut(s) 266, 434, 593, 635, 659, 808, 1221
RsaNI GTAC 7 cut(s) 265, 433, 592, 634, 658, 807, 1220
RseI CAYNNNNRTG 1 cut(s) 333
SaqAI TTAA 7 cut(s) 198, 236, 383, 540, 647, 852, 960
SatI GCNGC 2 cut(s) 1131, 1142
Sau3AI GATC 5 cut(s) 567, 715, 825, 1020, 1108
Sau96I GGNCC 2 cut(s) 750, 1095
ScaI AGTACT 1 cut(s) 808
SchI GAGTC 4 cut(s) 38, 466, 827, 918
ScrFI CCNGG 1 cut(s) 70
SduI GDGCHC 3 cut(s) 69, 110, 1198
SfaNI GCATC 3 cut(s) 140, 729, 1265
SfcI CTRYAG 2 cut(s) 13, 653
SinI GGWCC 1 cut(s) 1095
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 5 cut(s) 197, 745, 1034, 1051, 1100
SmoI CTYRAG 5 cut(s) 197, 745, 1034, 1051, 1100
SseBI AGGCCT 1 cut(s) 957
SspI AATATT 1 cut(s) 367
SspMI CTAG 1 cut(s) 686
StuI AGGCCT 1 cut(s) 957
StyD4I CCNGG 1 cut(s) 68
TaaI ACNGT 4 cut(s) 432, 517, 657, 1291
TaiI ACGT 3 cut(s) 169, 306, 837
TaqI TCGA 2 cut(s) 1023, 1299
TatI WGTACW 3 cut(s) 432, 633, 806
TfiI GAWTC 2 cut(s) 18, 1234
Tru1I TTAA 7 cut(s) 198, 236, 383, 540, 647, 852, 960
Tru9I TTAA 7 cut(s) 198, 236, 383, 540, 647, 852, 960
TscAI CASTG 3 cut(s) 1140, 1281, 1294
TseFI GTSAC 4 cut(s) 243, 469, 580, 1323
TseI GCWGC 2 cut(s) 1130, 1141
Tsp45I GTSAC 4 cut(s) 243, 469, 580, 1323
TspDTI ATGAA 4 cut(s) 166, 1043, 1233, 1332
TspGWI ACGGA 1 cut(s) 200
TspRI CASTG 3 cut(s) 1140, 1281, 1294
Vha464I CTTAAG 1 cut(s) 197
VpaK11BI GGWCC 1 cut(s) 1095
VspI ATTAAT 1 cut(s) 647
XagI CCTNNNNNAGG 1 cut(s) 951
XapI RAATTY 5 cut(s) 38, 114, 148, 605, 976
XceI RCATGY 1 cut(s) 61
XcmI CCANNNNNNNNNTGG 1 cut(s) 455
XmnI GAANNNNTTC 1 cut(s) 693
XspI CTAG 1 cut(s) 686
ZrmI AGTACT 1 cut(s) 808
Zsp2I ATGCAT 1 cut(s) 1258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.