Rmu_sc0002435.1_g000011

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002435.1
OG0002062
Physical Location & Seq
Forward (+)
45839 .. 47122
1284 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1284 bp
atgttcaattggaccacatcgttggagcaaagaatcgcagaaacagaatggttattacacccatcagccggaaacgagtcttgttgcatcttcagagtgcctcaactctataaggatacaaacaagaagtcctacgagcctcgtatagtctccattggtccatatcaccacggtaaaaaacatttgaagatgatgcagcagcacaaatggagatttcttcacgatctccttactcgaacaccatcaactggctcaagacttgatgactacctgcgcgaggtaacatcaatggaagaagtcatacgaaattgctattcagagacactcaaatttagcaaccgagagcttgttgagatgatggtgttggatggtctctttattattgaagtgttttgcaagatagaaaaattatcaccaatgggtaatggtgaccccatcttcaacatggcatgggtcatcattcagctcatccaagatcttttactgttagagaaccaaattccattcatggttcttcaaaaattgtttgatacatcaagatcttccagaaaagatagtaactcaacacttgccgaactttctctgaaattctttggcaactatgaattacgaataccttatggaaatgctcaaggaagacattttcttgatctacttcgcttgaattttatccctagggaaatgaggctccccagtaaacctcgagaagattatgaagttcaattatgtatccagtcagctacaaagcttcatctagctggagtaaagttcaagccaaggaaggcaaggagcttcctagatatcaaattttctgatggagtccttgaaatcccagaggtaagactaggcgatcactctaccgatctattcctgaatttcattgcatttgaacaatgctactatcattctccaagacacataactacttatgttgtattcatgtgttccctaatccgcacgcctaaggacgtagagtttctctgtgataatagtattatcgagaactattcatcttatgaggatattgcaatttttttcagaaacgtgggcaaaggtgtgacttttgattttgaacagggtcacctactgaatctgttcaacgatgtaaatgagtaccaaaaaaatatttgtcacgtgcgatgggcagaatttaggttcaagtacttcgattcaccttggtctttcttgtctgctttagctgctgtcatactcttaatattcactgcaattcaagccttctttgctgtttatgcatatttgttcccaccagaatctggtaaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

427

Amino Acids

50.03

Weight (kDa)

6.9

Isoelectric Point (pI)

35.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 30 - 407 8.5e-119 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 279
AccB7I CCANNNNNTGG 2 cut(s) 248, 1274
AccII CGCG 1 cut(s) 276
AciI CCGC 1 cut(s) 955
AcsI RAATTY 7 cut(s) 329, 498, 587, 664, 806, 874, 1148
AcuI CTGAAG 1 cut(s) 76
AcvI CACGTG 1 cut(s) 1135
AfaI GTAC 2 cut(s) 1115, 1163
AfiI CCNNNNNNNGG 4 cut(s) 68, 248, 277, 1274
AluBI AGCT 7 cut(s) 346, 466, 740, 748, 758, 792, 1199
AluI AGCT 7 cut(s) 346, 466, 740, 748, 758, 792, 1199
Alw26I GTCTC 3 cut(s) 154, 314, 377
AlwNI CAGNNNCTG 1 cut(s) 1274
Ama87I CYCGRG 1 cut(s) 702
ApeKI GCWGC 3 cut(s) 196, 199, 1199
ApoI RAATTY 7 cut(s) 329, 498, 587, 664, 806, 874, 1148
ArsI GACNNNNNNTTYG 2 cut(s) 113, 145
Asp700I GAANNNNTTC 1 cut(s) 1094
AspA2I CCTAGG 1 cut(s) 674
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 2 cut(s) 12, 158
AsuHPI GGTGA 5 cut(s) 158, 405, 440, 1073, 1164
AvaI CYCGRG 1 cut(s) 702
AvaII GGWCC 2 cut(s) 12, 158
AvrII CCTAGG 1 cut(s) 674
AxyI CCTNAGG 1 cut(s) 963
BarI GAAGNNNNNNTAC 2 cut(s) 285, 317
BbrPI CACGTG 1 cut(s) 1135
BbsI GAAGAC 1 cut(s) 643
BbvI GCAGC 3 cut(s) 208, 211, 1186
BccI CCATC 7 cut(s) 70, 250, 352, 362, 443, 809, 1134
BciVI GTATCC 2 cut(s) 109, 740
BcoDI GTCTC 3 cut(s) 154, 314, 377
BfaI CTAG 4 cut(s) 675, 755, 797, 845
BfuAI ACCTGC 1 cut(s) 279
BfuI GTATCC 2 cut(s) 109, 740
BglII AGATCT 2 cut(s) 475, 539
BisI GCNGC 3 cut(s) 197, 200, 1200
BlnI CCTAGG 1 cut(s) 674
BlsI GCNGC 3 cut(s) 198, 201, 1201
BmcAI AGTACT 1 cut(s) 1163
Bme18I GGWCC 2 cut(s) 12, 158
BmeT110I CYCGRG 1 cut(s) 702
BmgT120I GGNCC 2 cut(s) 12, 158
BmiI GGNNCC 1 cut(s) 689
BmrI ACTGGG 1 cut(s) 687
BmsI GCATC 2 cut(s) 96, 183
BmuI ACTGGG 1 cut(s) 687
BpiI GAAGAC 1 cut(s) 643
BpmI CTGGAG 1 cut(s) 780
BpuEI CTTGAG 2 cut(s) 238, 615
BsaAI YACGTR 1 cut(s) 1135
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 4 cut(s) 169, 674, 776, 1175
Bsc4I CCNNNNNNNGG 4 cut(s) 68, 248, 277, 1274
Bse1I ACTGG 3 cut(s) 253, 693, 733
Bse21I CCTNAGG 1 cut(s) 963
Bse3DI GCAATG 1 cut(s) 879
BseDI CCNNGG 4 cut(s) 169, 674, 776, 1175
BseGI GGATG 2 cut(s) 373, 468
BseLI CCNNNNNNNGG 4 cut(s) 68, 248, 277, 1274
BseMI GCAATG 1 cut(s) 879
BseNI ACTGG 3 cut(s) 253, 693, 733
BseXI GCAGC 3 cut(s) 208, 211, 1186
Bsh1236I CGCG 1 cut(s) 276
BsiHKCI CYCGRG 1 cut(s) 702
BsiSI CCGG 1 cut(s) 69
BslI CCNNNNNNNGG 4 cut(s) 68, 248, 277, 1274
BsmAI GTCTC 3 cut(s) 154, 314, 377
Bso31I GGTCTC 1 cut(s) 377
BsoBI CYCGRG 1 cut(s) 702
Bsp143I GATC 6 cut(s) 223, 475, 539, 649, 850, 862
BspACI CCGC 1 cut(s) 955
BspFNI CGCG 1 cut(s) 276
BspLI GGNNCC 1 cut(s) 689
BspMI ACCTGC 1 cut(s) 279
BspTNI GGTCTC 1 cut(s) 377
BsrDI GCAATG 1 cut(s) 879
BsrI ACTGG 3 cut(s) 253, 693, 733
BssECI CCNNGG 4 cut(s) 169, 674, 776, 1175
BssMI GATC 6 cut(s) 223, 475, 539, 649, 850, 862
BssT1I CCWWGG 3 cut(s) 674, 776, 1175
Bst4CI ACNGT 2 cut(s) 173, 486
BstBAI YACGTR 1 cut(s) 1135
BstC8I GCNNGC 1 cut(s) 959
BstDEI CTNAG 1 cut(s) 963
BstDSI CCRYGG 1 cut(s) 169
BstEII GGTNACC 2 cut(s) 428, 1079
BstENI CCTNNNNNAGG 1 cut(s) 275
BstF5I GGATG 2 cut(s) 373, 468
BstFNI CGCG 1 cut(s) 276
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 6 cut(s) 226, 478, 542, 652, 853, 865
BstMAI GTCTC 3 cut(s) 154, 314, 377
BstMBI GATC 6 cut(s) 223, 475, 539, 649, 850, 862
BstMWI GCNNNNNNNGC 4 cut(s) 1199, 1232, 1241, 1250
BstPI GGTNACC 2 cut(s) 428, 1079
BstUI CGCG 1 cut(s) 276
BstV1I GCAGC 3 cut(s) 208, 211, 1186
BstV2I GAAGAC 1 cut(s) 643
BstX2I RGATCY 2 cut(s) 475, 539
BstXI CCANNNNNNTGG 1 cut(s) 22
BstYI RGATCY 2 cut(s) 475, 539
Bsu36I CCTNAGG 1 cut(s) 963
BsuI GTATCC 2 cut(s) 109, 740
BtgI CCRYGG 1 cut(s) 169
BtgZI GCGATG 1 cut(s) 1153
BtsCI GGATG 2 cut(s) 373, 468
BtsI GCAGTG 1 cut(s) 1221
BtsIMutI CAGTG 1 cut(s) 1221
BveI ACCTGC 1 cut(s) 279
Cac8I GCNNGC 1 cut(s) 959
CaiI CAGNNNCTG 1 cut(s) 1274
CfoI GCGC 1 cut(s) 276
Cfr13I GGNCC 2 cut(s) 12, 158
Csp6I GTAC 2 cut(s) 1114, 1162
CviAII CATG 4 cut(s) 445, 450, 508, 940
CviQI GTAC 2 cut(s) 1114, 1162
DdeI CTNAG 1 cut(s) 963
DpnI GATC 6 cut(s) 225, 477, 541, 651, 852, 864
DpnII GATC 6 cut(s) 223, 475, 539, 649, 850, 862
Eco130I CCWWGG 3 cut(s) 674, 776, 1175
Eco31I GGTCTC 1 cut(s) 377
Eco32I GATATC 1 cut(s) 802
Eco47I GGWCC 2 cut(s) 12, 158
Eco57I CTGAAG 1 cut(s) 76
Eco72I CACGTG 1 cut(s) 1135
Eco81I CCTNAGG 1 cut(s) 963
Eco88I CYCGRG 1 cut(s) 702
Eco91I GGTNACC 2 cut(s) 428, 1079
EcoNI CCTNNNNNAGG 1 cut(s) 275
EcoO65I GGTNACC 2 cut(s) 428, 1079
EcoRV GATATC 1 cut(s) 802
EcoT14I CCWWGG 3 cut(s) 674, 776, 1175
EcoT22I ATGCAT 1 cut(s) 1255
ErhI CCWWGG 3 cut(s) 674, 776, 1175
FaeI CATG 4 cut(s) 448, 453, 511, 943
FatI CATG 4 cut(s) 444, 449, 507, 939
Fnu4HI GCNGC 3 cut(s) 197, 200, 1200
FokI GGATG 2 cut(s) 380, 455
Fsp4HI GCNGC 3 cut(s) 197, 200, 1200
FspBI CTAG 4 cut(s) 675, 755, 797, 845
GlaI GCGC 1 cut(s) 275
GluI GCNGC 3 cut(s) 197, 200, 1200
GsuI CTGGAG 1 cut(s) 780
HapII CCGG 1 cut(s) 69
HhaI GCGC 1 cut(s) 276
Hin1II CATG 4 cut(s) 448, 453, 511, 943
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 746
HinfI GANTC 6 cut(s) 33, 77, 819, 1090, 1169, 1271
HpaII CCGG 1 cut(s) 69
HphI GGTGA 5 cut(s) 158, 405, 440, 1073, 1164
Hpy166II GTNNAC 1 cut(s) 698
Hpy188I TCNGA 5 cut(s) 95, 319, 585, 814, 1040
Hpy188III TCNNGA 8 cut(s) 221, 255, 537, 546, 647, 704, 871, 1000
Hpy8I GTNNAC 1 cut(s) 698
HpyAV CCTTC 2 cut(s) 775, 1246
HpyCH4III ACNGT 2 cut(s) 173, 486
HpyCH4IV ACGT 3 cut(s) 969, 1044, 1134
HpyCH4V TGCA 7 cut(s) 87, 196, 396, 884, 1028, 1226, 1253
HpyF10VI GCNNNNNNNGC 4 cut(s) 1199, 1232, 1241, 1250
HpyF3I CTNAG 1 cut(s) 963
HpySE526I ACGT 3 cut(s) 969, 1044, 1134
Hsp92II CATG 4 cut(s) 448, 453, 511, 943
HspAI GCGC 1 cut(s) 274
Kzo9I GATC 6 cut(s) 223, 475, 539, 649, 850, 862
LmnI GCTCC 3 cut(s) 25, 693, 789
Lsp1109I GCAGC 3 cut(s) 208, 211, 1186
LweI GCATC 2 cut(s) 96, 183
MaeI CTAG 4 cut(s) 675, 755, 797, 845
MaeII ACGT 3 cut(s) 969, 1044, 1134
MaeIII GTNAC 6 cut(s) 280, 428, 557, 1057, 1079, 1130
MalI GATC 6 cut(s) 225, 477, 541, 651, 852, 864
MboI GATC 6 cut(s) 223, 475, 539, 649, 850, 862
MboII GAAGA 9 cut(s) 82, 199, 209, 305, 430, 506, 534, 648, 719
MfeI CAATTG 1 cut(s) 7
MflI RGATCY 2 cut(s) 475, 539
MlyI GAGTC 2 cut(s) 86, 828
MmeI TCCRAC 1 cut(s) 345
MnlI CCTC 7 cut(s) 111, 150, 271, 678, 711, 829, 1012
Mph1103I ATGCAT 1 cut(s) 1255
MroXI GAANNNNTTC 1 cut(s) 1094
MseI TTAA 1 cut(s) 1214
MspI CCGG 1 cut(s) 69
MunI CAATTG 1 cut(s) 7
MvnI CGCG 1 cut(s) 276
MwoI GCNNNNNNNGC 4 cut(s) 1199, 1232, 1241, 1250
NdeII GATC 6 cut(s) 223, 475, 539, 649, 850, 862
NlaIII CATG 4 cut(s) 448, 453, 511, 943
NlaIV GGNNCC 1 cut(s) 689
NmuCI GTSAC 4 cut(s) 428, 1057, 1079, 1130
NsiI ATGCAT 1 cut(s) 1255
PaeR7I CTCGAG 1 cut(s) 702
PdmI GAANNNNTTC 1 cut(s) 1094
PfeI GAWTC 4 cut(s) 33, 1090, 1169, 1271
PflMI CCANNNNNTGG 2 cut(s) 248, 1274
PkrI GCNGC 3 cut(s) 198, 201, 1201
PleI GAGTC 2 cut(s) 85, 827
PmaCI CACGTG 1 cut(s) 1135
PmlI CACGTG 1 cut(s) 1135
PpsI GAGTC 2 cut(s) 85, 827
Ppu21I YACGTR 1 cut(s) 1135
PspCI CACGTG 1 cut(s) 1135
PspEI GGTNACC 2 cut(s) 428, 1079
PspN4I GGNNCC 1 cut(s) 689
PspPI GGNCC 2 cut(s) 12, 158
PstNI CAGNNNCTG 1 cut(s) 1274
PsuI RGATCY 2 cut(s) 475, 539
RsaI GTAC 2 cut(s) 1115, 1163
RsaNI GTAC 2 cut(s) 1114, 1162
SaqAI TTAA 1 cut(s) 1214
SatI GCNGC 3 cut(s) 197, 200, 1200
Sau3AI GATC 6 cut(s) 223, 475, 539, 649, 850, 862
Sau96I GGNCC 2 cut(s) 12, 158
ScaI AGTACT 1 cut(s) 1163
SchI GAGTC 2 cut(s) 86, 828
SfaNI GCATC 2 cut(s) 96, 183
Sfr274I CTCGAG 1 cut(s) 702
SinI GGWCC 2 cut(s) 12, 158
SlaI CTCGAG 1 cut(s) 702
SmlI CTYRAG 3 cut(s) 253, 630, 702
SmoI CTYRAG 3 cut(s) 253, 630, 702
SsiI CCGC 1 cut(s) 955
SspI AATATT 2 cut(s) 1126, 1218
SspMI CTAG 4 cut(s) 675, 755, 797, 845
StyI CCWWGG 3 cut(s) 674, 776, 1175
TaaI ACNGT 2 cut(s) 173, 486
TaiI ACGT 3 cut(s) 972, 1047, 1137
TaqI TCGA 4 cut(s) 235, 703, 999, 1167
TatI WGTACW 1 cut(s) 1161
TfiI GAWTC 4 cut(s) 33, 1090, 1169, 1271
Tru1I TTAA 1 cut(s) 1214
Tru9I TTAA 1 cut(s) 1214
TscAI CASTG 1 cut(s) 1228
TseFI GTSAC 4 cut(s) 428, 1057, 1079, 1130
TseI GCWGC 3 cut(s) 196, 199, 1199
Tsp45I GTSAC 4 cut(s) 428, 1057, 1079, 1130
TspDTI ATGAA 7 cut(s) 496, 618, 729, 740, 868, 928, 999
TspRI CASTG 1 cut(s) 1228
Van91I CCANNNNNTGG 2 cut(s) 248, 1274
VpaK11BI GGWCC 2 cut(s) 12, 158
XagI CCTNNNNNAGG 1 cut(s) 275
XapI RAATTY 7 cut(s) 329, 498, 587, 664, 806, 874, 1148
XcmI CCANNNNNNNNNTGG 1 cut(s) 442
XhoI CTCGAG 1 cut(s) 702
XmaJI CCTAGG 1 cut(s) 674
XmnI GAANNNNTTC 1 cut(s) 1094
XspI CTAG 4 cut(s) 675, 755, 797, 845
ZrmI AGTACT 1 cut(s) 1163
Zsp2I ATGCAT 1 cut(s) 1255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.