Rmu_sc0003983.1_g000004

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003983.1
OG0002274
Physical Location & Seq
Forward (+)
25504 .. 26430
927 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 927 bp
atgatggacatcttcctctcgacattgctaaggaagaagggtaccacacaattgtccgtttatttgaggatcatctctttgctgaaaatgaaaaccggcggttggttatggcgaatagagcttcgctggaccttgcatatttgcccagcagttcgttttcttccaagccaagtcatgctgcagattcacatctacctagacaccaatgatgaggaagaagagcgtcaagtggctatggcaattatggtctctttggagcctgatttgcaagaaggaatcatcccacagtggtcattttggctggatgaaccaattggagaggcatctagcagttctacacctttgcaacgaggtcaagctacaggttcacagtcactgtcatctgctcaacaaattccaaatgattccagtagcagtcgagaatgcaagtactgttatgatgctccaatcgaagcagcttgtgatccctgcgggcatgatgcttgcttcaagctacaggttccactaccatctccaccactaactgttccaccactagcagcacttcctgaagaagaagacaccaatgatgaggaagaagagcgtcaagtgggtatggcaattatggtctctttggagcctgatttgcaagaaggaatcatcccacaatggtcattttggctggatgaaccaattggagaggcacctagcagttctacacctttgcaacgaggtcaagctacaggttcacagtcactgtcatctgctcaacaacattccggtagcagtcgagaatgcgagtactgttatgatgctccgatcgaagcagcttgtgatccctgtggacatgttgcttgcttcaactgctggctaaagattcaggactctgcaaacaagtgccctctttgtcgagtagagatcaagaagattatcaagctttatatgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

308

Amino Acids

34.35

Weight (kDa)

4.61

Isoelectric Point (pI)

79.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 256 - 302 3.2e-10 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 41
AccB1I GGYRCC 2 cut(s) 41, 682
AciI CCGC 2 cut(s) 99, 471
AclWI GGATC 3 cut(s) 77, 458, 809
AcsI RAATTY 1 cut(s) 393
AcuI CTGAAG 1 cut(s) 570
AfaI GTAC 3 cut(s) 43, 431, 782
AfiI CCNNNNNNNGG 1 cut(s) 102
AflIII ACRYGT 1 cut(s) 826
AgsI TTSAA 2 cut(s) 490, 841
AluBI AGCT 7 cut(s) 121, 359, 458, 493, 719, 809, 916
AluI AGCT 7 cut(s) 121, 359, 458, 493, 719, 809, 916
Alw26I GTCTC 2 cut(s) 253, 613
AlwI GGATC 3 cut(s) 77, 458, 809
AlwNI CAGNNNCTG 2 cut(s) 376, 736
ApeKI GCWGC 4 cut(s) 178, 455, 539, 806
ApoI RAATTY 1 cut(s) 393
Asp718I GGTACC 1 cut(s) 41
AspS9I GGNCC 1 cut(s) 129
AvaII GGWCC 1 cut(s) 129
BaeGI GKGCMC 1 cut(s) 881
BanI GGYRCC 2 cut(s) 41, 682
BbsI GAAGAC 1 cut(s) 564
BbvI GCAGC 4 cut(s) 165, 467, 551, 818
BccI CCATC 1 cut(s) 517
BcoDI GTCTC 2 cut(s) 253, 613
BfaI CTAG 4 cut(s) 197, 327, 536, 687
BfmI CTRYAG 4 cut(s) 179, 360, 494, 720
BisI GCNGC 4 cut(s) 179, 456, 540, 807
BlsI GCNGC 4 cut(s) 180, 457, 541, 808
BmcAI AGTACT 2 cut(s) 431, 782
Bme18I GGWCC 1 cut(s) 129
BmgT120I GGNCC 1 cut(s) 129
BmiI GGNNCC 5 cut(s) 43, 258, 501, 618, 684
BmsI GCATC 4 cut(s) 332, 430, 469, 781
BpiI GAAGAC 1 cut(s) 564
Bpu10I CCTNAGC 1 cut(s) 29
BsaBI GATNNNNATC 2 cut(s) 8, 188
BsaI GGTCTC 2 cut(s) 253, 613
BsaWI WCCGGW 1 cut(s) 758
Bsc4I CCNNNNNNNGG 1 cut(s) 102
Bse118I RCCGGY 1 cut(s) 95
Bse1I ACTGG 1 cut(s) 408
Bse3DI GCAATG 1 cut(s) 23
Bse8I GATNNNNATC 2 cut(s) 8, 188
BseGI GGATG 4 cut(s) 279, 310, 639, 670
BseJI GATNNNNATC 2 cut(s) 8, 188
BseLI CCNNNNNNNGG 1 cut(s) 102
BseMI GCAATG 1 cut(s) 23
BseNI ACTGG 1 cut(s) 408
BseSI GKGCMC 1 cut(s) 881
BseXI GCAGC 4 cut(s) 165, 467, 551, 818
BseYI CCCAGC 1 cut(s) 145
Bsh1285I CGRYCG 1 cut(s) 801
BshNI GGYRCC 2 cut(s) 41, 682
BsiEI CGRYCG 1 cut(s) 801
BsiSI CCGG 2 cut(s) 96, 759
BslI CCNNNNNNNGG 1 cut(s) 102
BsmAI GTCTC 2 cut(s) 253, 613
BsmI GAATGC 2 cut(s) 428, 779
Bso31I GGTCTC 2 cut(s) 253, 613
Bsp1286I GDGCHC 1 cut(s) 881
Bsp143I GATC 5 cut(s) 69, 463, 798, 814, 897
BspACI CCGC 2 cut(s) 99, 471
BspLI GGNNCC 5 cut(s) 43, 258, 501, 618, 684
BspMAI CTGCAG 1 cut(s) 183
BspPI GGATC 3 cut(s) 77, 458, 809
BspQI GCTCTTC 2 cut(s) 213, 573
BspT107I GGYRCC 2 cut(s) 41, 682
BspTNI GGTCTC 2 cut(s) 253, 613
BsrDI GCAATG 1 cut(s) 23
BsrFI RCCGGY 1 cut(s) 95
BsrI ACTGG 1 cut(s) 408
BssAI RCCGGY 1 cut(s) 95
BssMI GATC 5 cut(s) 69, 463, 798, 814, 897
Bst4CI ACNGT 8 cut(s) 288, 372, 378, 434, 526, 732, 738, 785
Bst6I CTCTTC 2 cut(s) 213, 573
BstC8I GCNNGC 4 cut(s) 473, 484, 835, 848
BstDEI CTNAG 1 cut(s) 29
BstF5I GGATG 4 cut(s) 279, 310, 639, 670
BstKTI GATC 5 cut(s) 72, 466, 801, 817, 900
BstMAI GTCTC 2 cut(s) 253, 613
BstMBI GATC 5 cut(s) 69, 463, 798, 814, 897
BstMCI CGRYCG 1 cut(s) 801
BstMWI GCNNNNNNNGC 4 cut(s) 118, 265, 625, 843
BstNSI RCATGY 1 cut(s) 830
BstSFI CTRYAG 4 cut(s) 179, 360, 494, 720
BstSLI GKGCMC 1 cut(s) 881
BstV1I GCAGC 4 cut(s) 165, 467, 551, 818
BstV2I GAAGAC 1 cut(s) 564
BtsCI GGATG 4 cut(s) 279, 310, 639, 670
BtsIMutI CAGTG 3 cut(s) 293, 374, 734
Cac8I GCNNGC 4 cut(s) 473, 484, 835, 848
CaiI CAGNNNCTG 2 cut(s) 376, 736
Cfr10I RCCGGY 1 cut(s) 95
Cfr13I GGNCC 1 cut(s) 129
CseI GACGC 2 cut(s) 212, 572
Csp6I GTAC 3 cut(s) 42, 430, 781
CviAII CATG 3 cut(s) 175, 476, 827
CviQI GTAC 3 cut(s) 42, 430, 781
DdeI CTNAG 1 cut(s) 29
DpnI GATC 5 cut(s) 71, 465, 800, 816, 899
DpnII GATC 5 cut(s) 69, 463, 798, 814, 897
Eam1104I CTCTTC 2 cut(s) 213, 573
EarI CTCTTC 2 cut(s) 213, 573
Eco31I GGTCTC 2 cut(s) 253, 613
Eco47I GGWCC 1 cut(s) 129
Eco57I CTGAAG 1 cut(s) 570
FaeI CATG 3 cut(s) 178, 479, 830
FatI CATG 3 cut(s) 174, 475, 826
FauI CCCGC 1 cut(s) 464
Fnu4HI GCNGC 4 cut(s) 179, 456, 540, 807
FokI GGATG 4 cut(s) 266, 317, 626, 677
Fsp4HI GCNGC 4 cut(s) 179, 456, 540, 807
FspBI CTAG 4 cut(s) 197, 327, 536, 687
GluI GCNGC 4 cut(s) 179, 456, 540, 807
GsaI CCCAGC 1 cut(s) 149
HapII CCGG 2 cut(s) 96, 759
HgaI GACGC 2 cut(s) 212, 572
Hin1II CATG 3 cut(s) 178, 479, 830
HindIII AAGCTT 1 cut(s) 914
HinfI GANTC 6 cut(s) 184, 276, 404, 636, 856, 863
HpaII CCGG 2 cut(s) 96, 759
Hpy166II GTNNAC 3 cut(s) 368, 728, 824
Hpy188I TCNGA 1 cut(s) 798
Hpy188III TCNNGA 6 cut(s) 19, 419, 548, 770, 860, 901
Hpy8I GTNNAC 3 cut(s) 368, 728, 824
HpyAV CCTTC 3 cut(s) 31, 266, 626
HpyCH4III ACNGT 8 cut(s) 288, 372, 378, 434, 526, 732, 738, 785
HpyCH4V TGCA 8 cut(s) 136, 181, 268, 346, 426, 628, 706, 869
HpyF10VI GCNNNNNNNGC 4 cut(s) 118, 265, 625, 843
HpyF3I CTNAG 1 cut(s) 29
Hsp92II CATG 3 cut(s) 178, 479, 830
KpnI GGTACC 1 cut(s) 45
Kzo9I GATC 5 cut(s) 69, 463, 798, 814, 897
LguI GCTCTTC 2 cut(s) 213, 573
LmnI GCTCC 4 cut(s) 256, 448, 616, 799
Lsp1109I GCAGC 4 cut(s) 165, 467, 551, 818
LweI GCATC 4 cut(s) 332, 430, 469, 781
MaeI CTAG 4 cut(s) 197, 327, 536, 687
MaeIII GTNAC 2 cut(s) 372, 732
MalI GATC 5 cut(s) 71, 465, 800, 816, 899
MboI GATC 5 cut(s) 69, 463, 798, 814, 897
MfeI CAATTG 3 cut(s) 50, 312, 672
MhlI GDGCHC 1 cut(s) 881
MluCI AATT 6 cut(s) 50, 240, 312, 393, 600, 672
MlyI GAGTC 1 cut(s) 857
MnlI CCTC 9 cut(s) 26, 60, 205, 313, 344, 565, 673, 704, 891
MspI CCGG 2 cut(s) 96, 759
MunI CAATTG 3 cut(s) 50, 312, 672
Mva1269I GAATGC 2 cut(s) 428, 779
MwoI GCNNNNNNNGC 4 cut(s) 118, 265, 625, 843
NdeII GATC 5 cut(s) 69, 463, 798, 814, 897
NlaIII CATG 3 cut(s) 178, 479, 830
NlaIV GGNNCC 5 cut(s) 43, 258, 501, 618, 684
NmuCI GTSAC 2 cut(s) 372, 732
NspI RCATGY 1 cut(s) 830
PciI ACATGT 1 cut(s) 826
PciSI GCTCTTC 2 cut(s) 213, 573
PctI GAATGC 2 cut(s) 428, 779
PfeI GAWTC 5 cut(s) 184, 276, 404, 636, 856
PkrI GCNGC 4 cut(s) 180, 457, 541, 808
Ple19I CGATCG 1 cut(s) 801
PleI GAGTC 1 cut(s) 857
PpsI GAGTC 1 cut(s) 857
PscI ACATGT 1 cut(s) 826
PspFI CCCAGC 1 cut(s) 145
PspN4I GGNNCC 5 cut(s) 43, 258, 501, 618, 684
PspPI GGNCC 1 cut(s) 129
PstI CTGCAG 1 cut(s) 183
PstNI CAGNNNCTG 2 cut(s) 376, 736
PvuI CGATCG 1 cut(s) 801
RsaI GTAC 3 cut(s) 43, 431, 782
RsaNI GTAC 3 cut(s) 42, 430, 781
SapI GCTCTTC 2 cut(s) 213, 573
SatI GCNGC 4 cut(s) 179, 456, 540, 807
Sau3AI GATC 5 cut(s) 69, 463, 798, 814, 897
Sau96I GGNCC 1 cut(s) 129
ScaI AGTACT 2 cut(s) 431, 782
SchI GAGTC 1 cut(s) 857
SduI GDGCHC 1 cut(s) 881
SfaNI GCATC 4 cut(s) 332, 430, 469, 781
SfcI CTRYAG 4 cut(s) 179, 360, 494, 720
SinI GGWCC 1 cut(s) 129
Sse9I AATT 6 cut(s) 50, 240, 312, 393, 600, 672
SsiI CCGC 2 cut(s) 99, 471
SspMI CTAG 4 cut(s) 197, 327, 536, 687
TaaI ACNGT 8 cut(s) 288, 372, 378, 434, 526, 732, 738, 785
TaqI TCGA 6 cut(s) 20, 418, 450, 769, 801, 889
TasI AATT 6 cut(s) 50, 240, 312, 393, 600, 672
TatI WGTACW 2 cut(s) 429, 780
TfiI GAWTC 5 cut(s) 184, 276, 404, 636, 856
TscAI CASTG 3 cut(s) 293, 381, 741
TseFI GTSAC 2 cut(s) 372, 732
TseI GCWGC 4 cut(s) 178, 455, 539, 806
Tsp45I GTSAC 2 cut(s) 372, 732
TspDTI ATGAA 3 cut(s) 104, 321, 681
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 3 cut(s) 293, 381, 741
VpaK11BI GGWCC 1 cut(s) 129
XapI RAATTY 1 cut(s) 393
XceI RCATGY 1 cut(s) 830
XspI CTAG 4 cut(s) 197, 327, 536, 687
ZrmI AGTACT 2 cut(s) 431, 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.