Rmu_sc0005711.1_g000009

(E,E)-alpha-farnesene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005711.1
OG0002039
Physical Location & Seq
Forward (+)
63950 .. 66151
2202 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1506 bp
atgtttgctgattgtaaggaatcagatttaataaccaagttggagttgcttgacagcatccaaaaactaggcctgaacaaccattttggaaaggaaatcaagaaagccctagacaccataacatctgttgaactcgaaaataacagcaatccatacattagttcaagaggtgatctctatgctgctgcactactctttaagattcttaggcagcatggttacaaagtttcacaagatatatttggtggtttgatggatgaaatgggtacattaaagaagaaaacatttggggatgtgaaaggaatgcttgaaatgttggaggcctcaaacctagctttagaaggtgaatatatcctagaggaggctaaagctttcttaatggtcactttcagagataccaatactaggtgtgatgatatagacaattgcattttcaagcatgtgatttataccctggagcttccatcacaaaggagagtgcagtggtttaatgtgcaatggcacatcaaatcctacgagaaagaccatactgatcaagccaacatgaccactttacttgagttggctaaacttaacttcaacatggttcaagccacactccaaaaagatctaagggaagtatccatgtggtggaacaatctaggtctcaataagaacttggactttgcaagagatagactggttgaatgtttcatgtgtgccgtgggattagctttcgagcctgagtacaagtcctttagaaaatggataactaaagtcatcaatttaattctcatcatcgatgatgtttatgatgtttatggctcattagaagagctaaaacatttcaccaatgctgttaaaaggtgggatattagggaaaccgagcagcttccggagtgtatgaagatctgtttccaagtactctacaatactacttgtgaaattgcttatgaaattgaggaggagaaaggttggaaccaatggggagatttctgtaaagcattattggtggaggcagagtggtacaataaggcctacacaccgtcctttgaagaacaccttagcaatggttgcatttcatcatctactttgctgatgttgactcatgccttcttctccatgacacgtgttgaaggggccaaaaacattgctgattttctgcacaagaatgaagatcttgtgtacaatatctctttgattgttcgactcttaaacgatctgggaacttctgtggctgagcaaaagagaggtgatgttgcttcatcaatcctatgttacatgagagaagcgaatgcttcagaagaaatagctcgaaagcacatcaaaggcatgatagacaatgcatggaagaaaataaacgaaaaatgcttcactcaaatgcctgaaatatcttcattcttcaacattatgacaaatatagctcgaattgggcatagcctttatcaagatggagatggatttggtgatcaagagcacgggactcgggcacagatccaatctttactagttgaacctctctcgtga

Protein Analysis

501

Amino Acids

57.75

Weight (kDa)

5.24

Isoelectric Point (pI)

36.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 5 - 131 9.4e-34 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 213 - 445 2.8e-84 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 252 - 445 2.2e-50 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 630
AccIII TCCGGA 1 cut(s) 874
AclWI GGATC 1 cut(s) 1468
AcuI CTGAAG 1 cut(s) 1263
AcvI CACGTG 1 cut(s) 1109
AfaI GTAC 5 cut(s) 268, 728, 903, 1007, 1166
AfiI CCNNNNNNNGG 3 cut(s) 361, 405, 630
AflIII ACRYGT 2 cut(s) 1106, 1108
AhlI ACTAGT 1 cut(s) 1486
AjnI CCWGG 1 cut(s) 453
AluBI AGCT 8 cut(s) 335, 371, 460, 713, 817, 871, 1292, 1403
AluI AGCT 8 cut(s) 335, 371, 460, 713, 817, 871, 1292, 1403
Alw21I GWGCWC 1 cut(s) 1458
Alw26I GTCTC 1 cut(s) 650
AlwI GGATC 1 cut(s) 1468
Ama87I CYCGRG 1 cut(s) 1464
Aor13HI TCCGGA 1 cut(s) 874
AoxI GGCC 4 cut(s) 70, 321, 1014, 1119
ApeKI GCWGC 4 cut(s) 182, 185, 211, 868
AspS9I GGNCC 1 cut(s) 1119
AsuHPI GGTGA 5 cut(s) 182, 356, 820, 1244, 1457
AvaI CYCGRG 1 cut(s) 1464
BaeGI GKGCMC 1 cut(s) 1471
BauI CACGAG 1 cut(s) 1501
BbrPI CACGTG 1 cut(s) 1109
Bbv12I GWGCWC 1 cut(s) 1458
BbvI GCAGC 4 cut(s) 169, 172, 223, 880
BccI CCATC 4 cut(s) 247, 472, 1424, 1430
BceAI ACGGC 1 cut(s) 686
BcgI CGANNNNNNTGC 2 cut(s) 1445, 1479
BciT130I CCWGG 1 cut(s) 455
BciVI GTATCC 1 cut(s) 631
BclI TGATCA 2 cut(s) 532, 1447
BcoDI GTCTC 1 cut(s) 650
BcuI ACTAGT 1 cut(s) 1486
BfaI CTAG 7 cut(s) 68, 110, 332, 356, 405, 641, 1487
BfuI GTATCC 1 cut(s) 631
BglII AGATCT 3 cut(s) 607, 888, 1156
BisI GCNGC 4 cut(s) 183, 186, 212, 869
BlpI GCTNAGC 1 cut(s) 1218
BlsI GCNGC 4 cut(s) 184, 187, 213, 870
BmcAI AGTACT 1 cut(s) 903
Bme1390I CCNGG 1 cut(s) 455
BmeT110I CYCGRG 1 cut(s) 1464
BmgT120I GGNCC 1 cut(s) 1119
BmiI GGNNCC 2 cut(s) 959, 1120
BmrFI CCNGG 1 cut(s) 455
BmsI GCATC 1 cut(s) 66
BplI GAGNNNNNCTC 2 cut(s) 159, 191
BpmI CTGGAG 1 cut(s) 476
Bpu10I CCTNAGC 1 cut(s) 1043
Bpu1102I GCTNAGC 1 cut(s) 1218
BpuEI CTTGAG 1 cut(s) 578
Bsa29I ATCGAT 1 cut(s) 780
BsaAI YACGTR 1 cut(s) 1109
BsaI GGTCTC 1 cut(s) 650
BsaJI CCNNGG 2 cut(s) 453, 702
BsaWI WCCGGW 1 cut(s) 874
BsaXI ACNNNNNCTCC 2 cut(s) 986, 1016
Bsc4I CCNNNNNNNGG 3 cut(s) 361, 405, 630
Bse1I ACTGG 1 cut(s) 684
Bse3DI GCAATG 3 cut(s) 503, 1054, 1128
BseAI TCCGGA 1 cut(s) 874
BseBI CCWGG 1 cut(s) 455
BseCI ATCGAT 1 cut(s) 780
BseDI CCNNGG 2 cut(s) 453, 702
BseGI GGATG 3 cut(s) 57, 262, 298
BseLI CCNNNNNNNGG 3 cut(s) 361, 405, 630
BseMI GCAATG 3 cut(s) 503, 1054, 1128
BseMII CTCAG 2 cut(s) 714, 1209
BseNI ACTGG 1 cut(s) 684
BseRI GAGGAG 3 cut(s) 374, 956, 959
BseSI GKGCMC 1 cut(s) 1471
BseXI GCAGC 4 cut(s) 169, 172, 223, 880
BsgI GTGCAG 3 cut(s) 171, 500, 1127
BshFI GGCC 4 cut(s) 72, 323, 1016, 1121
BshVI ATCGAT 1 cut(s) 780
BsiHKAI GWGCWC 1 cut(s) 1458
BsiHKCI CYCGRG 1 cut(s) 1464
BsiSI CCGG 1 cut(s) 875
BslFI GGGAC 1 cut(s) 1474
BslI CCNNNNNNNGG 3 cut(s) 361, 405, 630
BsmAI GTCTC 1 cut(s) 650
BsmFI GGGAC 1 cut(s) 1474
BsmI GAATGC 2 cut(s) 309, 1279
BsnI GGCC 4 cut(s) 72, 323, 1016, 1121
Bso31I GGTCTC 1 cut(s) 650
BsoBI CYCGRG 1 cut(s) 1464
Bsp1286I GDGCHC 2 cut(s) 1458, 1471
Bsp13I TCCGGA 1 cut(s) 874
Bsp1407I TGTACA 1 cut(s) 1164
Bsp143I GATC 8 cut(s) 172, 532, 607, 888, 1156, 1198, 1447, 1473
Bsp1720I GCTNAGC 1 cut(s) 1218
BspANI GGCC 4 cut(s) 72, 323, 1016, 1121
BspCNI CTCAG 2 cut(s) 715, 1210
BspDI ATCGAT 1 cut(s) 780
BspEI TCCGGA 1 cut(s) 874
BspLI GGNNCC 2 cut(s) 959, 1120
BspPI GGATC 1 cut(s) 1468
BspQI GCTCTTC 1 cut(s) 807
BspTNI GGTCTC 1 cut(s) 650
BsrDI GCAATG 3 cut(s) 503, 1054, 1128
BsrGI TGTACA 1 cut(s) 1164
BsrI ACTGG 1 cut(s) 684
BssECI CCNNGG 2 cut(s) 453, 702
BssMI GATC 8 cut(s) 172, 532, 607, 888, 1156, 1198, 1447, 1473
BssSI CACGAG 1 cut(s) 1501
Bst2BI CACGAG 1 cut(s) 1501
Bst2UI CCWGG 1 cut(s) 455
Bst4CI ACNGT 1 cut(s) 1026
Bst6I CTCTTC 1 cut(s) 807
BstAPI GCANNNNNTGC 1 cut(s) 1053
BstAUI TGTACA 1 cut(s) 1164
BstBAI YACGTR 1 cut(s) 1109
BstDEI CTNAG 5 cut(s) 206, 611, 723, 1043, 1218
BstDSI CCRYGG 1 cut(s) 702
BstENI CCTNNNNNAGG 1 cut(s) 359
BstF5I GGATG 3 cut(s) 57, 262, 298
BstKTI GATC 8 cut(s) 175, 535, 610, 891, 1159, 1201, 1450, 1476
BstMAI GTCTC 1 cut(s) 650
BstMBI GATC 8 cut(s) 172, 532, 607, 888, 1156, 1198, 1447, 1473
BstMWI GCNNNNNNNGC 1 cut(s) 1053
BstNI CCWGG 1 cut(s) 455
BstNSI RCATGY 1 cut(s) 443
BstSCI CCNGG 1 cut(s) 453
BstSLI GKGCMC 1 cut(s) 1471
BstV1I GCAGC 4 cut(s) 169, 172, 223, 880
BstX2I RGATCY 4 cut(s) 607, 888, 1156, 1473
BstYI RGATCY 4 cut(s) 607, 888, 1156, 1473
Bsu15I ATCGAT 1 cut(s) 780
BsuI GTATCC 1 cut(s) 631
BsuRI GGCC 4 cut(s) 72, 323, 1016, 1121
BsuTUI ATCGAT 1 cut(s) 780
BtgI CCRYGG 1 cut(s) 702
BtsCI GGATG 3 cut(s) 57, 262, 298
BtsI GCAGTG 1 cut(s) 488
BtsIMutI CAGTG 1 cut(s) 488
Cfr13I GGNCC 1 cut(s) 1119
ClaI ATCGAT 1 cut(s) 780
Csp6I GTAC 5 cut(s) 267, 727, 902, 1006, 1165
CspCI CAANNNNNGTGG 2 cut(s) 538, 573
CviQI GTAC 5 cut(s) 267, 727, 902, 1006, 1165
DdeI CTNAG 5 cut(s) 206, 611, 723, 1043, 1218
DpnI GATC 8 cut(s) 174, 534, 609, 890, 1158, 1200, 1449, 1475
DpnII GATC 8 cut(s) 172, 532, 607, 888, 1156, 1198, 1447, 1473
Eam1104I CTCTTC 1 cut(s) 807
EarI CTCTTC 1 cut(s) 807
Eco147I AGGCCT 3 cut(s) 72, 323, 1016
Eco31I GGTCTC 1 cut(s) 650
Eco57I CTGAAG 1 cut(s) 1263
Eco72I CACGTG 1 cut(s) 1109
Eco88I CYCGRG 1 cut(s) 1464
EcoNI CCTNNNNNAGG 1 cut(s) 359
EcoRII CCWGG 1 cut(s) 453
EcoT22I ATGCAT 1 cut(s) 1327
FalI AAGNNNNNCTT 4 cut(s) 291, 323, 1026, 1058
FaqI GGGAC 1 cut(s) 1474
FbaI TGATCA 2 cut(s) 532, 1447
Fnu4HI GCNGC 4 cut(s) 183, 186, 212, 869
FokI GGATG 3 cut(s) 44, 269, 305
Fsp4HI GCNGC 4 cut(s) 183, 186, 212, 869
FspBI CTAG 7 cut(s) 68, 110, 332, 356, 405, 641, 1487
GluI GCNGC 4 cut(s) 183, 186, 212, 869
GsuI CTGGAG 1 cut(s) 476
HaeIII GGCC 4 cut(s) 72, 323, 1016, 1121
HapII CCGG 1 cut(s) 875
HincII GTYRAC 1 cut(s) 1083
HindII GTYRAC 1 cut(s) 1083
HindIII AAGCTT 1 cut(s) 369
HinfI GANTC 5 cut(s) 20, 202, 1084, 1188, 1462
HpaII CCGG 1 cut(s) 875
HphI GGTGA 5 cut(s) 182, 356, 820, 1244, 1457
Hpy166II GTNNAC 2 cut(s) 1083, 1165
Hpy188I TCNGA 3 cut(s) 25, 392, 1282
Hpy188III TCNNGA 6 cut(s) 100, 165, 875, 1427, 1451, 1503
Hpy8I GTNNAC 2 cut(s) 1083, 1165
HpyAV CCTTC 3 cut(s) 335, 1102, 1109
HpyCH4III ACNGT 1 cut(s) 1026
HpyCH4IV ACGT 1 cut(s) 1108
HpyCH4V TGCA 8 cut(s) 188, 429, 481, 496, 668, 1056, 1144, 1325
HpyF10VI GCNNNNNNNGC 1 cut(s) 1053
HpyF3I CTNAG 5 cut(s) 206, 611, 723, 1043, 1218
HpySE526I ACGT 1 cut(s) 1108
Kpn2I TCCGGA 1 cut(s) 874
Ksp22I TGATCA 2 cut(s) 532, 1447
Kzo9I GATC 8 cut(s) 172, 532, 607, 888, 1156, 1198, 1447, 1473
LguI GCTCTTC 1 cut(s) 807
LmnI GCTCC 1 cut(s) 457
LpnPI CCDG 8 cut(s) 86, 440, 467, 665, 735, 888, 1187, 1377
Lsp1109I GCAGC 4 cut(s) 169, 172, 223, 880
LweI GCATC 1 cut(s) 66
MaeI CTAG 7 cut(s) 68, 110, 332, 356, 405, 641, 1487
MaeII ACGT 1 cut(s) 1108
MaeIII GTNAC 3 cut(s) 218, 382, 1256
MalI GATC 8 cut(s) 174, 534, 609, 890, 1158, 1200, 1449, 1475
MboI GATC 8 cut(s) 172, 532, 607, 888, 1156, 1198, 1447, 1473
MfeI CAATTG 1 cut(s) 424
MflI RGATCY 4 cut(s) 607, 888, 1156, 1473
MhlI GDGCHC 2 cut(s) 1458, 1471
MluCI AATT 6 cut(s) 424, 763, 768, 924, 936, 1407
MlyI GAGTC 3 cut(s) 1078, 1182, 1456
MmeI TCCRAC 3 cut(s) 21, 297, 935
Mph1103I ATGCAT 1 cut(s) 1327
MroI TCCGGA 1 cut(s) 874
MseI TTAA 9 cut(s) 29, 198, 272, 377, 489, 573, 767, 840, 1193
MslI CAYNNNNRTG 2 cut(s) 1149, 1358
MspI CCGG 1 cut(s) 875
MspR9I CCNGG 1 cut(s) 455
MunI CAATTG 1 cut(s) 424
Mva1269I GAATGC 2 cut(s) 309, 1279
MvaI CCWGG 1 cut(s) 455
MwoI GCNNNNNNNGC 1 cut(s) 1053
NdeII GATC 8 cut(s) 172, 532, 607, 888, 1156, 1198, 1447, 1473
NlaIV GGNNCC 2 cut(s) 959, 1120
NmuCI GTSAC 1 cut(s) 382
NsiI ATGCAT 1 cut(s) 1327
NspI RCATGY 1 cut(s) 443
PceI AGGCCT 3 cut(s) 72, 323, 1016
PciSI GCTCTTC 1 cut(s) 807
PctI GAATGC 2 cut(s) 309, 1279
PfeI GAWTC 2 cut(s) 20, 202
PflMI CCANNNNNTGG 1 cut(s) 630
PkrI GCNGC 4 cut(s) 184, 187, 213, 870
PleI GAGTC 3 cut(s) 1078, 1182, 1456
PmaCI CACGTG 1 cut(s) 1109
PmlI CACGTG 1 cut(s) 1109
PpsI GAGTC 3 cut(s) 1078, 1182, 1456
Ppu21I YACGTR 1 cut(s) 1109
Psp6I CCWGG 1 cut(s) 453
PspCI CACGTG 1 cut(s) 1109
PspGI CCWGG 1 cut(s) 453
PspN4I GGNNCC 2 cut(s) 959, 1120
PspPI GGNCC 1 cut(s) 1119
PsuI RGATCY 4 cut(s) 607, 888, 1156, 1473
RsaI GTAC 5 cut(s) 268, 728, 903, 1007, 1166
RsaNI GTAC 5 cut(s) 267, 727, 902, 1006, 1165
RseI CAYNNNNRTG 2 cut(s) 1149, 1358
SapI GCTCTTC 1 cut(s) 807
SaqAI TTAA 9 cut(s) 29, 198, 272, 377, 489, 573, 767, 840, 1193
SatI GCNGC 4 cut(s) 183, 186, 212, 869
Sau3AI GATC 8 cut(s) 172, 532, 607, 888, 1156, 1198, 1447, 1473
Sau96I GGNCC 1 cut(s) 1119
ScaI AGTACT 1 cut(s) 903
SchI GAGTC 3 cut(s) 1078, 1182, 1456
ScrFI CCNGG 1 cut(s) 455
SduI GDGCHC 2 cut(s) 1458, 1471
SfaNI GCATC 1 cut(s) 66
SmiMI CAYNNNNRTG 2 cut(s) 1149, 1358
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
SpeI ACTAGT 1 cut(s) 1486
Sse9I AATT 6 cut(s) 424, 763, 768, 924, 936, 1407
SseBI AGGCCT 3 cut(s) 72, 323, 1016
SspMI CTAG 7 cut(s) 68, 110, 332, 356, 405, 641, 1487
StuI AGGCCT 3 cut(s) 72, 323, 1016
StyD4I CCNGG 1 cut(s) 453
TaaI ACNGT 1 cut(s) 1026
TaiI ACGT 1 cut(s) 1111
TaqI TCGA 6 cut(s) 135, 717, 780, 1186, 1294, 1405
TasI AATT 6 cut(s) 424, 763, 768, 924, 936, 1407
TatI WGTACW 3 cut(s) 726, 901, 1164
TfiI GAWTC 2 cut(s) 20, 202
Tru1I TTAA 9 cut(s) 29, 198, 272, 377, 489, 573, 767, 840, 1193
Tru9I TTAA 9 cut(s) 29, 198, 272, 377, 489, 573, 767, 840, 1193
TscAI CASTG 1 cut(s) 488
TseFI GTSAC 1 cut(s) 382
TseI GCWGC 4 cut(s) 182, 185, 211, 868
Tsp45I GTSAC 1 cut(s) 382
TspDTI ATGAA 8 cut(s) 273, 682, 899, 948, 1050, 1167, 1233, 1365
TspRI CASTG 1 cut(s) 488
Van91I CCANNNNNTGG 1 cut(s) 630
XagI CCTNNNNNAGG 1 cut(s) 359
XceI RCATGY 1 cut(s) 443
XspI CTAG 7 cut(s) 68, 110, 332, 356, 405, 641, 1487
ZrmI AGTACT 1 cut(s) 903
Zsp2I ATGCAT 1 cut(s) 1327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.