Rmu_sc0006181.1_g000012

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006181.1
OG0004260
Physical Location & Seq
Forward (+)
40113 .. 41504
1392 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 888 bp
atggccgcggctatggcggctcgactggcggcagcacttgcgctgacgaacgaagcggtggttgagtttggggaggctggagcagcggctgttgcagcgtcgggtttttatttggttgggcatatgttggcgcctagggtggaggatgatgcaggattgcctaaagtgatggcggcggtgtggggcctgcgtgatctcctctcgatcatgaaggttgatggtgaccagttccaattctggttttcggtgaaagaggtgggtcaaagtgttcttcatggtggcctgtggaatttcaagaacagtatgcttctgttggaggagtatgacgaactcggtgagccggagatggttccattgaactcactggaggagtgggtcactatgcagcgtctgccaccacgtctgccgactgacgaatctctgatgctgattagttcatcgatgggtaggttcctgagggcagagaagttttctttgcagcggggagcgccagagcaacgattccaggtccttctggatgttcgtcgacatctgtgggtgaagtggacctatagttttacggagacagccaaggcggaggtggagctgcattatgaaaaggatggtgctccatttccgctggttcccatggccttacaggcgggacatgtgagtggggttaagcgttcaccatctgggatgcaagttcgtccaggtaagaaggctaaattgcgtgctgattcctccgcccttgtgtgcatggatgtggaagaggaacaggctgtagcacaggtggttgagctacctgcaatggaagaagcaatttctgtgggaggtgaggtggtcattgaggaggtcagtgtgagtcctctcctgcaggaaatggctccgggtttagctcaagaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283

Protein Analysis

295

Amino Acids

32.29

Weight (kDa)

4.86

Isoelectric Point (pI)

49.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 37 - 110 1.2e-06 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 793
AccB1I GGYRCC 1 cut(s) 130
AccI GTMKAC 1 cut(s) 526
AccII CGCG 1 cut(s) 8
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 289
AcyI GRCGYC 1 cut(s) 131
AflIII ACRYGT 1 cut(s) 646
AgsI TTSAA 2 cut(s) 295, 358
AjiI CACGTC 1 cut(s) 401
AjnI CCWGG 2 cut(s) 504, 691
AluBI AGCT 3 cut(s) 586, 781, 878
AluI AGCT 3 cut(s) 586, 781, 878
Alw21I GWGCWC 1 cut(s) 610
Alw26I GTCTC 1 cut(s) 557
AlwNI CAGNNNCTG 2 cut(s) 89, 391
AoxI GGCC 4 cut(s) 3, 184, 280, 630
ApeKI GCWGC 6 cut(s) 32, 83, 95, 385, 478, 586
ApoI RAATTY 1 cut(s) 289
AspA2I CCTAGG 1 cut(s) 134
AspLEI GCGC 3 cut(s) 43, 133, 490
AspS9I GGNCC 3 cut(s) 184, 508, 546
AsuC2I CCSGG 1 cut(s) 870
AsuHPI GGTGA 6 cut(s) 233, 259, 347, 550, 660, 827
AvaII GGWCC 2 cut(s) 508, 546
AvrII CCTAGG 1 cut(s) 134
AxyI CCTNAGG 1 cut(s) 455
BanI GGYRCC 1 cut(s) 130
Bbv12I GWGCWC 1 cut(s) 610
BbvI GCAGC 6 cut(s) 44, 95, 107, 397, 490, 573
BccI CCATC 6 cut(s) 163, 212, 340, 436, 596, 679
BciT130I CCWGG 2 cut(s) 506, 693
BcnI CCSGG 1 cut(s) 870
BcoDI GTCTC 1 cut(s) 557
BfaI CTAG 1 cut(s) 135
BfmI CTRYAG 3 cut(s) 550, 762, 854
BfoI RGCGCY 2 cut(s) 134, 491
BfuAI ACCTGC 1 cut(s) 793
BglI GCCNNNNNGGC 1 cut(s) 638
BlnI CCTAGG 1 cut(s) 134
Bme1390I CCNGG 3 cut(s) 506, 693, 870
Bme18I GGWCC 2 cut(s) 508, 546
BmgBI CACGTC 1 cut(s) 401
BmgT120I GGNCC 3 cut(s) 184, 508, 546
BmiI GGNNCC 6 cut(s) 132, 185, 351, 452, 624, 867
BmrFI CCNGG 3 cut(s) 506, 693, 870
BmsI GCATC 3 cut(s) 139, 414, 669
BpmI CTGGAG 2 cut(s) 99, 386
BpuEI CTTGAG 1 cut(s) 864
BpuMI CCSGG 1 cut(s) 870
Bsa29I ATCGAT 1 cut(s) 440
BsaHI GRCGYC 1 cut(s) 131
BsaJI CCNNGG 4 cut(s) 6, 134, 570, 627
BsaXI ACNNNNNCTCC 2 cut(s) 804, 834
Bse1I ACTGG 3 cut(s) 30, 226, 369
Bse21I CCTNAGG 1 cut(s) 455
Bse3DI GCAATG 1 cut(s) 795
BseBI CCWGG 2 cut(s) 506, 693
BseCI ATCGAT 1 cut(s) 440
BseDI CCNNGG 4 cut(s) 6, 134, 570, 627
BseGI GGATG 5 cut(s) 151, 523, 607, 684, 748
BseMI GCAATG 1 cut(s) 795
BseMII CTCAG 1 cut(s) 446
BseNI ACTGG 3 cut(s) 30, 226, 369
BseRI GAGGAG 4 cut(s) 188, 332, 383, 845
BseXI GCAGC 6 cut(s) 44, 95, 107, 397, 490, 573
Bsh1236I CGCG 1 cut(s) 8
BshFI GGCC 4 cut(s) 5, 186, 282, 632
BshNI GGYRCC 1 cut(s) 130
BshVI ATCGAT 1 cut(s) 440
BsiHKAI GWGCWC 1 cut(s) 610
BsiSI CCGG 2 cut(s) 341, 869
BslFI GGGAC 1 cut(s) 657
BsmAI GTCTC 1 cut(s) 557
BsmFI GGGAC 1 cut(s) 657
BsnI GGCC 4 cut(s) 5, 186, 282, 632
Bsp1286I GDGCHC 1 cut(s) 610
Bsp143I GATC 2 cut(s) 193, 204
Bsp19I CCATGG 1 cut(s) 627
BspANI GGCC 4 cut(s) 5, 186, 282, 632
BspCNI CTCAG 1 cut(s) 447
BspDI ATCGAT 1 cut(s) 440
BspFNI CGCG 1 cut(s) 8
BspHI TCATGA 1 cut(s) 207
BspLI GGNNCC 6 cut(s) 132, 185, 351, 452, 624, 867
BspMAI CTGCAG 1 cut(s) 858
BspMI ACCTGC 1 cut(s) 793
BspT107I GGYRCC 1 cut(s) 130
BsrDI GCAATG 1 cut(s) 795
BsrI ACTGG 3 cut(s) 30, 226, 369
BssECI CCNNGG 4 cut(s) 6, 134, 570, 627
BssMI GATC 2 cut(s) 193, 204
BssNI GRCGYC 1 cut(s) 131
BssT1I CCWWGG 3 cut(s) 134, 570, 627
Bst2UI CCWGG 2 cut(s) 506, 693
Bst4CI ACNGT 1 cut(s) 302
Bst6I CTCTTC 1 cut(s) 744
BstACI GRCGYC 1 cut(s) 131
BstAPI GCANNNNNTGC 2 cut(s) 38, 391
BstC8I GCNNGC 2 cut(s) 188, 714
BstDEI CTNAG 1 cut(s) 455
BstDSI CCRYGG 2 cut(s) 6, 627
BstEII GGTNACC 1 cut(s) 221
BstF5I GGATG 5 cut(s) 151, 523, 607, 684, 748
BstFNI CGCG 1 cut(s) 8
BstH2I RGCGCY 2 cut(s) 134, 491
BstHHI GCGC 3 cut(s) 43, 133, 490
BstKTI GATC 2 cut(s) 196, 207
BstMAI GTCTC 1 cut(s) 557
BstMBI GATC 2 cut(s) 193, 204
BstNI CCWGG 2 cut(s) 506, 693
BstNSI RCATGY 1 cut(s) 650
BstPI GGTNACC 1 cut(s) 221
BstSCI CCNGG 3 cut(s) 504, 691, 868
BstSFI CTRYAG 3 cut(s) 550, 762, 854
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 6 cut(s) 44, 95, 107, 397, 490, 573
Bsu15I ATCGAT 1 cut(s) 440
Bsu36I CCTNAGG 1 cut(s) 455
BsuRI GGCC 4 cut(s) 5, 186, 282, 632
BsuTUI ATCGAT 1 cut(s) 440
BtgI CCRYGG 2 cut(s) 6, 627
BtrI CACGTC 1 cut(s) 401
BtsCI GGATG 5 cut(s) 151, 523, 607, 684, 748
BtsIMutI CAGTG 2 cut(s) 362, 844
BveI ACCTGC 1 cut(s) 793
Cac8I GCNNGC 2 cut(s) 188, 714
CaiI CAGNNNCTG 2 cut(s) 89, 391
CciI TCATGA 1 cut(s) 207
CfoI GCGC 3 cut(s) 43, 133, 490
Cfr13I GGNCC 3 cut(s) 184, 508, 546
Cfr42I CCGCGG 1 cut(s) 9
ClaI ATCGAT 1 cut(s) 440
CseI GACGC 2 cut(s) 87, 377
CspCI CAANNNNNGTGG 2 cut(s) 789, 824
CviAII CATG 5 cut(s) 208, 275, 628, 647, 739
DdeI CTNAG 1 cut(s) 455
DinI GGCGCC 1 cut(s) 132
DpnI GATC 2 cut(s) 195, 206
DpnII GATC 2 cut(s) 193, 204
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 744
EciI GGCGGA 2 cut(s) 590, 715
Eco130I CCWWGG 3 cut(s) 134, 570, 627
Eco47I GGWCC 2 cut(s) 508, 546
Eco81I CCTNAGG 1 cut(s) 455
Eco91I GGTNACC 1 cut(s) 221
EcoO109I RGGNCCY 2 cut(s) 184, 508
EcoO65I GGTNACC 1 cut(s) 221
EcoRII CCWGG 2 cut(s) 504, 691
EcoT14I CCWWGG 3 cut(s) 134, 570, 627
EgeI GGCGCC 1 cut(s) 132
EheI GGCGCC 1 cut(s) 132
ErhI CCWWGG 3 cut(s) 134, 570, 627
FaeI CATG 5 cut(s) 211, 278, 631, 650, 742
FaqI GGGAC 1 cut(s) 657
FatI CATG 5 cut(s) 207, 274, 627, 646, 738
FauI CCCGC 2 cut(s) 474, 634
FauNDI CATATG 1 cut(s) 123
FblI GTMKAC 1 cut(s) 526
FokI GGATG 5 cut(s) 158, 530, 614, 691, 755
FspBI CTAG 1 cut(s) 135
GlaI GCGC 3 cut(s) 42, 132, 489
GsuI CTGGAG 2 cut(s) 99, 386
HaeII RGCGCY 2 cut(s) 134, 491
HaeIII GGCC 4 cut(s) 5, 186, 282, 632
HapII CCGG 2 cut(s) 341, 869
HgaI GACGC 2 cut(s) 87, 377
HhaI GCGC 3 cut(s) 43, 133, 490
Hin1I GRCGYC 1 cut(s) 131
Hin1II CATG 5 cut(s) 211, 278, 631, 650, 742
Hin6I GCGC 3 cut(s) 41, 131, 488
HinP1I GCGC 3 cut(s) 41, 131, 488
HincII GTYRAC 1 cut(s) 527
HindII GTYRAC 1 cut(s) 527
HinfI GANTC 4 cut(s) 416, 501, 719, 844
HpaII CCGG 2 cut(s) 341, 869
HphI GGTGA 6 cut(s) 233, 259, 347, 550, 660, 827
Hpy166II GTNNAC 3 cut(s) 527, 546, 668
Hpy188I TCNGA 1 cut(s) 423
Hpy188III TCNNGA 6 cut(s) 202, 208, 295, 454, 515, 881
Hpy8I GTNNAC 3 cut(s) 527, 546, 668
Hpy99I CGWCG 2 cut(s) 103, 528
HpyAV CCTTC 3 cut(s) 205, 521, 694
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4IV ACGT 1 cut(s) 400
HpyCH4V TGCA 9 cut(s) 95, 152, 385, 478, 589, 682, 738, 788, 856
HpyF3I CTNAG 1 cut(s) 455
HpySE526I ACGT 1 cut(s) 400
Hsp92I GRCGYC 1 cut(s) 131
Hsp92II CATG 5 cut(s) 211, 278, 631, 650, 742
HspAI GCGC 3 cut(s) 41, 131, 488
KasI GGCGCC 1 cut(s) 130
KspI CCGCGG 1 cut(s) 9
Kzo9I GATC 2 cut(s) 193, 204
LmnI GCTCC 5 cut(s) 80, 485, 583, 613, 871
Lsp1109I GCAGC 6 cut(s) 44, 95, 107, 397, 490, 573
LweI GCATC 3 cut(s) 139, 414, 669
MaeI CTAG 1 cut(s) 135
MaeII ACGT 1 cut(s) 400
MaeIII GTNAC 2 cut(s) 221, 376
MalI GATC 2 cut(s) 195, 206
MboI GATC 2 cut(s) 193, 204
MboII GAAGA 3 cut(s) 263, 761, 806
MhlI GDGCHC 1 cut(s) 610
MluCI AATT 4 cut(s) 233, 289, 707, 801
Mly113I GGCGCC 1 cut(s) 131
MlyI GAGTC 1 cut(s) 853
MmeI TCCRAC 1 cut(s) 294
MseI TTAA 1 cut(s) 660
MslI CAYNNNNRTG 2 cut(s) 651, 743
MspA1I CMGCKG 4 cut(s) 8, 86, 481, 619
MspI CCGG 2 cut(s) 341, 869
MspR9I CCNGG 3 cut(s) 506, 693, 870
MvaI CCWGG 2 cut(s) 506, 693
MvnI CGCG 1 cut(s) 8
NarI GGCGCC 1 cut(s) 131
NciI CCSGG 1 cut(s) 870
NcoI CCATGG 1 cut(s) 627
NdeI CATATG 1 cut(s) 123
NdeII GATC 2 cut(s) 193, 204
NlaIII CATG 5 cut(s) 211, 278, 631, 650, 742
NlaIV GGNNCC 6 cut(s) 132, 185, 351, 452, 624, 867
NmuCI GTSAC 2 cut(s) 221, 376
NspI RCATGY 1 cut(s) 650
PagI TCATGA 1 cut(s) 207
PciI ACATGT 1 cut(s) 646
PfeI GAWTC 3 cut(s) 416, 501, 719
PleI GAGTC 1 cut(s) 852
PluTI GGCGCC 1 cut(s) 134
PpsI GAGTC 1 cut(s) 852
PpuMI RGGWCCY 1 cut(s) 508
PscI ACATGT 1 cut(s) 646
Psp5II RGGWCCY 1 cut(s) 508
Psp6I CCWGG 2 cut(s) 504, 691
PspEI GGTNACC 1 cut(s) 221
PspGI CCWGG 2 cut(s) 504, 691
PspN4I GGNNCC 6 cut(s) 132, 185, 351, 452, 624, 867
PspPI GGNCC 3 cut(s) 184, 508, 546
PspPPI RGGWCCY 1 cut(s) 508
PstI CTGCAG 1 cut(s) 858
PstNI CAGNNNCTG 2 cut(s) 89, 391
RseI CAYNNNNRTG 2 cut(s) 651, 743
SacII CCGCGG 1 cut(s) 9
SalI GTCGAC 1 cut(s) 525
SaqAI TTAA 1 cut(s) 660
Sau3AI GATC 2 cut(s) 193, 204
Sau96I GGNCC 3 cut(s) 184, 508, 546
SbfI CCTGCAGG 1 cut(s) 858
SchI GAGTC 1 cut(s) 853
ScrFI CCNGG 3 cut(s) 506, 693, 870
SdaI CCTGCAGG 1 cut(s) 858
SduI GDGCHC 1 cut(s) 610
SfaNI GCATC 3 cut(s) 139, 414, 669
SfcI CTRYAG 3 cut(s) 550, 762, 854
SfoI GGCGCC 1 cut(s) 132
Sfr303I CCGCGG 1 cut(s) 9
SgrBI CCGCGG 1 cut(s) 9
SinI GGWCC 2 cut(s) 508, 546
SmiMI CAYNNNNRTG 2 cut(s) 651, 743
SmlI CTYRAG 1 cut(s) 879
SmoI CTYRAG 1 cut(s) 879
Sse8387I CCTGCAGG 1 cut(s) 858
Sse9I AATT 4 cut(s) 233, 289, 707, 801
SspDI GGCGCC 1 cut(s) 130
SspMI CTAG 1 cut(s) 135
StyD4I CCNGG 3 cut(s) 504, 691, 868
StyI CCWWGG 3 cut(s) 134, 570, 627
TaaI ACNGT 1 cut(s) 302
TaiI ACGT 1 cut(s) 403
TaqI TCGA 4 cut(s) 22, 203, 440, 526
TasI AATT 4 cut(s) 233, 289, 707, 801
TauI GCSGC 6 cut(s) 8, 11, 20, 32, 89, 176
TfiI GAWTC 3 cut(s) 416, 501, 719
Tru1I TTAA 1 cut(s) 660
Tru9I TTAA 1 cut(s) 660
TscAI CASTG 2 cut(s) 369, 844
TseFI GTSAC 2 cut(s) 221, 376
TseI GCWGC 6 cut(s) 32, 83, 95, 385, 478, 586
Tsp45I GTSAC 2 cut(s) 221, 376
TspDTI ATGAA 4 cut(s) 224, 263, 426, 609
TspGWI ACGGA 1 cut(s) 575
TspRI CASTG 2 cut(s) 369, 844
VpaK11BI GGWCC 2 cut(s) 508, 546
XapI RAATTY 1 cut(s) 289
XceI RCATGY 1 cut(s) 650
XcmI CCANNNNNNNNNTGG 1 cut(s) 577
XmaJI CCTAGG 1 cut(s) 134
XmiI GTMKAC 1 cut(s) 526
XspI CTAG 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.