Rmu_sc0006445.1_g000018
ERF Family

Thioesterase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006445.1
OG0002384
Physical Location & Seq
Reverse (-)
70740 .. 73714
2975 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 738 bp
atggcaaagctcccaattgctctctcagagagctttgcttccaattccagagcaacaatttccaaagatattcctgaagaacacttgaaccaactgttgaggtttcttgaaagagtggggatctctggcaatgtcccagattattgcaataccgaagacttctattccgacctcattggtggcatcctcaagcccctgacagtcctacgaggccgcatcacttgcttggtctctgtaaaacctgctgttaccaataaatttggaggatttcatggaggagctattgctgctgttgctgaggctgtgtcgtttgctactgcaagaacgattgtggctgaggataaggagctttttctaggggaactaagcatctcttacctctcttctgcaccaaagaatgcagaggtgataattgatggatctgtagtgcggagtgggagaaacttaactgtggtagcactggagttcaagctgaagaaaactgggaacttgatgtacactgctcgtgctaccttatatcacatgcccgtctcgaaattcaccgccgtgatgacgtcgaccgagacggaggacgccgtcgcaccgccatcaccgattaccaagagctcgagtcctgagtttgcgaaggtcagattcgtactccaaatcttccttattcatgttcgattctgcaaccggagagcgtttctctcgccatcggcgaaccagttcacaagcaattcgaaagtccagaggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

245

Amino Acids

26.68

Weight (kDa)

9.4

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT PF03061 88 - 161 7.8e-09 Thioesterase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 557
Acc36I ACCTGC 1 cut(s) 250
AccI GTMKAC 1 cut(s) 557
AciI CCGC 4 cut(s) 214, 430, 543, 584
AclWI GGATC 2 cut(s) 128, 427
AcsI RAATTY 2 cut(s) 257, 536
AcuI CTGAAG 2 cut(s) 96, 494
AcyI GRCGYC 2 cut(s) 554, 573
AfaI GTAC 2 cut(s) 497, 639
AgsI TTSAA 3 cut(s) 88, 110, 469
AleI CACNNNNGTG 1 cut(s) 545
AluBI AGCT 6 cut(s) 10, 33, 281, 349, 472, 606
AluI AGCT 6 cut(s) 10, 33, 281, 349, 472, 606
Alw21I GWGCWC 1 cut(s) 608
Alw26I GTCTC 3 cut(s) 235, 535, 557
AlwI GGATC 2 cut(s) 128, 427
Ama87I CYCGRG 1 cut(s) 607
AoxI GGCC 1 cut(s) 211
ApeKI GCWGC 1 cut(s) 287
ApoI RAATTY 2 cut(s) 257, 536
Asp700I GAANNNNTTC 1 cut(s) 707
AsuHPI GGTGA 3 cut(s) 418, 532, 582
AsuII TTCGAA 1 cut(s) 722
AvaI CYCGRG 1 cut(s) 607
BanII GRGCYC 1 cut(s) 608
BauI CACGAG 1 cut(s) 504
BbsI GAAGAC 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 608
BbvCI CCTCAGC 2 cut(s) 297, 336
BbvI GCAGC 1 cut(s) 274
BccI CCATC 3 cut(s) 410, 595, 703
BceAI ACGGC 2 cut(s) 530, 560
BcoDI GTCTC 3 cut(s) 235, 535, 557
BfaI CTAG 1 cut(s) 356
BfmI CTRYAG 1 cut(s) 423
BfuAI ACCTGC 1 cut(s) 250
BisI GCNGC 2 cut(s) 214, 288
BlsI GCNGC 2 cut(s) 215, 289
BmeT110I CYCGRG 1 cut(s) 607
BmrI ACTGGG 1 cut(s) 492
BmsI GCATC 3 cut(s) 192, 225, 378
BmuI ACTGGG 1 cut(s) 492
BpiI GAAGAC 1 cut(s) 162
BplI GAGNNNNNCTC 2 cut(s) 672, 704
BpmI CTGGAG 1 cut(s) 482
Bpu10I CCTNAGC 2 cut(s) 297, 336
Bpu14I TTCGAA 1 cut(s) 722
BpuEI CTTGAG 1 cut(s) 173
BsaHI GRCGYC 2 cut(s) 554, 573
BsaI GGTCTC 1 cut(s) 235
BsaWI WCCGGW 1 cut(s) 675
Bse1I ACTGG 3 cut(s) 465, 487, 706
Bse3DI GCAATG 1 cut(s) 136
BseGI GGATG 1 cut(s) 183
BseMI GCAATG 1 cut(s) 136
BseMII CTCAG 4 cut(s) 39, 288, 327, 606
BseNI ACTGG 3 cut(s) 465, 487, 706
BseRI GAGGAG 1 cut(s) 291
BseXI GCAGC 1 cut(s) 274
BsgI GTGCAG 1 cut(s) 372
Bsh1285I CGRYCG 1 cut(s) 561
BshFI GGCC 1 cut(s) 213
BsiEI CGRYCG 1 cut(s) 561
BsiHKAI GWGCWC 1 cut(s) 608
BsiHKCI CYCGRG 1 cut(s) 607
BsiSI CCGG 1 cut(s) 676
BslFI GGGAC 1 cut(s) 119
BsmAI GTCTC 3 cut(s) 235, 535, 557
BsmBI CGTCTC 2 cut(s) 535, 557
BsmFI GGGAC 1 cut(s) 119
BsmI GAATGC 1 cut(s) 403
BsnI GGCC 1 cut(s) 213
Bso31I GGTCTC 1 cut(s) 235
BsoBI CYCGRG 1 cut(s) 607
Bsp119I TTCGAA 1 cut(s) 722
Bsp1286I GDGCHC 1 cut(s) 608
Bsp1407I TGTACA 1 cut(s) 495
Bsp143I GATC 2 cut(s) 120, 419
BspACI CCGC 4 cut(s) 214, 430, 543, 584
BspANI GGCC 1 cut(s) 213
BspCNI CTCAG 4 cut(s) 38, 289, 328, 607
BspMI ACCTGC 1 cut(s) 250
BspPI GGATC 2 cut(s) 128, 427
BspT104I TTCGAA 1 cut(s) 722
BspTNI GGTCTC 1 cut(s) 235
BsrDI GCAATG 1 cut(s) 136
BsrGI TGTACA 1 cut(s) 495
BsrI ACTGG 3 cut(s) 465, 487, 706
BssMI GATC 2 cut(s) 120, 419
BssNI GRCGYC 2 cut(s) 554, 573
BssSI CACGAG 1 cut(s) 504
Bst2BI CACGAG 1 cut(s) 504
Bst4CI ACNGT 3 cut(s) 96, 202, 451
Bst6I CTCTTC 1 cut(s) 388
BstACI GRCGYC 2 cut(s) 554, 573
BstAPI GCANNNNNTGC 1 cut(s) 222
BstAUI TGTACA 1 cut(s) 495
BstBI TTCGAA 1 cut(s) 722
BstDEI CTNAG 5 cut(s) 25, 297, 336, 365, 615
BstF5I GGATG 1 cut(s) 183
BstKTI GATC 2 cut(s) 123, 422
BstMAI GTCTC 3 cut(s) 235, 535, 557
BstMBI GATC 2 cut(s) 120, 419
BstMCI CGRYCG 1 cut(s) 561
BstMWI GCNNNNNNNGC 3 cut(s) 222, 287, 293
BstNSI RCATGY 1 cut(s) 526
BstSFI CTRYAG 1 cut(s) 423
BstV1I GCAGC 1 cut(s) 274
BstV2I GAAGAC 1 cut(s) 162
BstX2I RGATCY 2 cut(s) 120, 419
BstYI RGATCY 2 cut(s) 120, 419
BsuRI GGCC 1 cut(s) 213
BtsCI GGATG 1 cut(s) 183
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 2 cut(s) 458, 498
BveI ACCTGC 1 cut(s) 250
CseI GACGC 1 cut(s) 581
Csp6I GTAC 2 cut(s) 496, 638
CviAII CATG 3 cut(s) 272, 523, 659
CviQI GTAC 2 cut(s) 496, 638
DdeI CTNAG 5 cut(s) 25, 297, 336, 365, 615
DpnI GATC 2 cut(s) 122, 421
DpnII GATC 2 cut(s) 120, 419
Eam1104I CTCTTC 1 cut(s) 388
EarI CTCTTC 1 cut(s) 388
Ecl136II GAGCTC 1 cut(s) 606
Eco24I GRGCYC 1 cut(s) 608
Eco31I GGTCTC 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 606
Eco57I CTGAAG 2 cut(s) 96, 494
Eco88I CYCGRG 1 cut(s) 607
EcoICRI GAGCTC 1 cut(s) 606
EcoT38I GRGCYC 1 cut(s) 608
Esp3I CGTCTC 2 cut(s) 535, 557
FaeI CATG 3 cut(s) 275, 526, 662
FaiI YATR 4 cut(s) 273, 517, 524, 660
FalI AAGNNNNNCTT 2 cut(s) 358, 390
FaqI GGGAC 1 cut(s) 119
FatI CATG 3 cut(s) 271, 522, 658
FblI GTMKAC 1 cut(s) 557
Fnu4HI GCNGC 2 cut(s) 214, 288
FokI GGATG 1 cut(s) 170
FriOI GRGCYC 1 cut(s) 608
Fsp4HI GCNGC 2 cut(s) 214, 288
FspBI CTAG 1 cut(s) 356
GluI GCNGC 2 cut(s) 214, 288
GsuI CTGGAG 1 cut(s) 482
HaeIII GGCC 1 cut(s) 213
HapII CCGG 1 cut(s) 676
HgaI GACGC 1 cut(s) 581
Hin1I GRCGYC 2 cut(s) 554, 573
Hin1II CATG 3 cut(s) 275, 526, 662
HincII GTYRAC 1 cut(s) 558
HindII GTYRAC 1 cut(s) 558
HinfI GANTC 3 cut(s) 610, 633, 666
HpaII CCGG 1 cut(s) 676
HphI GGTGA 3 cut(s) 418, 532, 582
Hpy166II GTNNAC 3 cut(s) 498, 558, 711
Hpy188I TCNGA 3 cut(s) 28, 169, 632
Hpy188III TCNNGA 6 cut(s) 48, 74, 107, 532, 614, 730
Hpy8I GTNNAC 3 cut(s) 498, 558, 711
Hpy99I CGWCG 2 cut(s) 559, 581
HpyAV CCTTC 1 cut(s) 619
HpyCH4III ACNGT 3 cut(s) 96, 202, 451
HpyCH4IV ACGT 1 cut(s) 554
HpyCH4V TGCA 5 cut(s) 147, 320, 389, 401, 672
HpyF10VI GCNNNNNNNGC 3 cut(s) 222, 287, 293
HpyF3I CTNAG 5 cut(s) 25, 297, 336, 365, 615
HpySE526I ACGT 1 cut(s) 554
Hsp92I GRCGYC 2 cut(s) 554, 573
Hsp92II CATG 3 cut(s) 275, 526, 662
Kzo9I GATC 2 cut(s) 120, 419
LmnI GCTCC 3 cut(s) 15, 278, 346
Lsp1109I GCAGC 1 cut(s) 274
LweI GCATC 3 cut(s) 192, 225, 378
MaeI CTAG 1 cut(s) 356
MaeII ACGT 1 cut(s) 554
MaeIII GTNAC 1 cut(s) 247
MalI GATC 2 cut(s) 122, 421
MboI GATC 2 cut(s) 120, 419
MboII GAAGA 5 cut(s) 89, 167, 375, 487, 640
MfeI CAATTG 1 cut(s) 15
MflI RGATCY 2 cut(s) 120, 419
MhlI GDGCHC 1 cut(s) 608
MluCI AATT 7 cut(s) 15, 43, 57, 257, 411, 536, 718
MlyI GAGTC 1 cut(s) 619
MmeI TCCRAC 1 cut(s) 192
MroXI GAANNNNTTC 1 cut(s) 707
MseI TTAA 1 cut(s) 446
MslI CAYNNNNRTG 1 cut(s) 545
MspI CCGG 1 cut(s) 676
MunI CAATTG 1 cut(s) 15
Mva1269I GAATGC 1 cut(s) 403
MwoI GCNNNNNNNGC 3 cut(s) 222, 287, 293
NdeII GATC 2 cut(s) 120, 419
NlaIII CATG 3 cut(s) 275, 526, 662
NspI RCATGY 1 cut(s) 526
NspV TTCGAA 1 cut(s) 722
OliI CACNNNNGTG 1 cut(s) 545
PaeR7I CTCGAG 1 cut(s) 607
PcsI WCGNNNNNNNCGW 1 cut(s) 698
PctI GAATGC 1 cut(s) 403
PdmI GAANNNNTTC 1 cut(s) 707
PfeI GAWTC 2 cut(s) 633, 666
PflFI GACNNNGTC 1 cut(s) 575
PkrI GCNGC 2 cut(s) 215, 289
PleI GAGTC 1 cut(s) 618
PpsI GAGTC 1 cut(s) 618
Psp124BI GAGCTC 1 cut(s) 608
PspXI VCTCGAGB 1 cut(s) 607
PsrI GAACNNNNNNTAC 2 cut(s) 479, 511
PsuI RGATCY 2 cut(s) 120, 419
PsyI GACNNNGTC 1 cut(s) 575
RsaI GTAC 2 cut(s) 497, 639
RsaNI GTAC 2 cut(s) 496, 638
RseI CAYNNNNRTG 1 cut(s) 545
SacI GAGCTC 1 cut(s) 608
SalI GTCGAC 1 cut(s) 556
SaqAI TTAA 1 cut(s) 446
SatI GCNGC 2 cut(s) 214, 288
Sau3AI GATC 2 cut(s) 120, 419
SchI GAGTC 1 cut(s) 619
SduI GDGCHC 1 cut(s) 608
SfaNI GCATC 3 cut(s) 192, 225, 378
SfcI CTRYAG 1 cut(s) 423
Sfr274I CTCGAG 1 cut(s) 607
SfuI TTCGAA 1 cut(s) 722
SlaI CTCGAG 1 cut(s) 607
SmiMI CAYNNNNRTG 1 cut(s) 545
SmlI CTYRAG 2 cut(s) 188, 607
SmoI CTYRAG 2 cut(s) 188, 607
Sse9I AATT 7 cut(s) 15, 43, 57, 257, 411, 536, 718
SsiI CCGC 4 cut(s) 214, 430, 543, 584
SspMI CTAG 1 cut(s) 356
SstI GAGCTC 1 cut(s) 608
TaaI ACNGT 3 cut(s) 96, 202, 451
TaiI ACGT 1 cut(s) 557
TaqI TCGA 5 cut(s) 533, 557, 608, 664, 722
TaqII GACCGA 1 cut(s) 575
TasI AATT 7 cut(s) 15, 43, 57, 257, 411, 536, 718
TatI WGTACW 1 cut(s) 495
TauI GCSGC 1 cut(s) 216
TfiI GAWTC 2 cut(s) 633, 666
Tru1I TTAA 1 cut(s) 446
Tru9I TTAA 1 cut(s) 446
TscAI CASTG 2 cut(s) 465, 505
TseI GCWGC 1 cut(s) 287
TspDTI ATGAA 2 cut(s) 260, 647
TspGWI ACGGA 1 cut(s) 581
TspRI CASTG 2 cut(s) 465, 505
Tth111I GACNNNGTC 1 cut(s) 575
XapI RAATTY 2 cut(s) 257, 536
XceI RCATGY 1 cut(s) 526
XhoI CTCGAG 1 cut(s) 607
XmiI GTMKAC 1 cut(s) 557
XmnI GAANNNNTTC 1 cut(s) 707
XspI CTAG 1 cut(s) 356
ZraI GACGTC 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.