Rmu_sc0007237.1_g000003

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007237.1
OG0001584
Physical Location & Seq
Reverse (-)
4858 .. 18727
13870 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2571 bp
atggctggggcgaaagaagagatattgaaagcccgtacggacaacagggagtacaggaggatcgtcctccctaactcccttgaagtcctcctcatcagcgatcctgatactaacaagtgtgctgcttccatggatgtcagtgttggttctttcagcgaccctgatggcgtggagggccttgcccatttccttgagcatatgctgttttatgctagtgagaagtaccctttggaggacagctacttgaagtacatcatcgagcatggaggaagaggaaatgctttcacttctgaagaacacaccagctatcgtttcgacattaatcccgatggttttgaagaggctctggacaggtttgctcagttctttattaaacccttgatgtcagctgatgctacagcgaggaaaattaaagctgttaattcaggcaattggaatactttgtgggttcgaccaaaagcaaaaggattggataccagacatgaacttattaaattctatgaagaatactattcagccaacctcatgcatctggttatatacggaaaagagaaacttgataaaattcaaggccttgtagaggacaagtttaaggaaattcgaaatattgaccgaagttgccttcgttttactggtgagccttgcacatcagaacatctggagattcttgtcagaaccgtcccaataaaagagggtcataaattaagacttgcatggcctataactccagaaattcaccattacaaggaagggccgtgcatgtatcttggtcaccttatcggccatgaaggagaaggatctttatattacatcttgaaaacattgggatgggcaacaggtttgcgggctggtgaatcatattctactttggacttttctttctttgaagtagatattgatctcaccgatgcaggccacgacattatccaaattgtgctgaacaagctttctccaaacaatgtccgaatcttctgggagtccaagaaatttgaaggtgatacaaacatgtttgagccctggtatggaactgcttactgcatggaaaaaattacgggctccagaattcaggaatggatagcatcttctccaaatgtgaatttacacctaccagcacgtaatgtgttcatccccacggacttgtctcttaagaatgagaatgagaaggtcaaatgtccagttctgttaagaaagtcaccatatgcaacactatggtacaagcctgatacaatgttttttactcctaaagcatacgtcaagattgatttcaattgtccccatgcaagtggctccccggaagcagaagtcctgactagtatttttactcatttgttaatggattacttgaatgattatgcttattatgctgaggttgctggactgtactgtggaatataccacacagaaagtggttttcaggtgactttggttggttataatcacaaattgaggattttactggaagcggtagttgagaagattgcaagctttaaagtgaaagctgacaggttctctgtgatcaaggaaacggtcacaaaggagtaccaaaattacaaatttgaacagtcctatgagcaggctatttactattgctcattagttctacaggaccagacttggccattgatggaacaactagaagttcttactcatcttgaagttgaagatcttgctaagtttgttccattgatgctctcaagggccttcatagtgtgttacacagcaggaaaccttgaaagtactgaagctgagtcaatgatcctgcacgtagaagatgttttttttaagggttcaaaccgtatttgccaacctttattcccatcccaacatttgacaaatagagtcgtgaagcttgaaaaggggaaaaactttatctatcctgtggaaggccttaatccaagtgataagaattcttcccttgtccactatatccaggttcatcgagatgattttatgctgaatgtgaaacttcagctatttgttcttattgcaaagcaaccagcctttcaccaacttagaatagttgagcaacttggttacataactcgtctttcacagaggaatgattgtggtatccgtggactgcagttcattatacaatccacagtgaagggtccaggacgtattgatttgagagtggaggaatttctcaagacgtttgagagtaagctttatgagatgacaaataacgaattcaagagcaatgtaaatactttgatcgatatgaagcttgagaagcaccagaacttgagggaagaagctgaattttattggagggagatttctgatgggaccctcaagtttgatcgtaaagaagcagagattgcagcactgaggcagcttacacaacaagagttgattgagtttttcaatgagcatataaaagttggggcacctcataagaggacattgagtgtacgagtatatgggaatcgtcattcatttgagtatgcatcagataaaagatccctagtgcaaccttgcaccgtcaaaatcgatgatattttcgctttcagaaggtcacagcctctttatggttcattcaagggaagctccggtcatgtgaagttgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

856

Amino Acids

98.77

Weight (kDa)

6.22

Isoelectric Point (pI)

36.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 29 - 140 3.8e-30 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 159 - 314 2e-23 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 306 - 546 5.6e-86 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 551 - 737 9.6e-11 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 662 - 757 9.6e-21 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1434
AccB1I GGYRCC 1 cut(s) 2389
AciI CCGC 2 cut(s) 844, 1463
AclWI GGATC 5 cut(s) 68, 95, 805, 1750, 2457
AcoI YGGCCR 2 cut(s) 781, 1616
AcuI CTGAAG 3 cut(s) 312, 1761, 1952
AfaI GTAC 9 cut(s) 37, 53, 224, 251, 1214, 1382, 1541, 1738, 2415
AfiI CCNNNNNNNGG 6 cut(s) 232, 580, 745, 1022, 1883, 2531
AflII CTTAAG 1 cut(s) 1145
AflIII ACRYGT 1 cut(s) 1005
AhlI ACTAGT 1 cut(s) 1310
AjnI CCWGG 3 cut(s) 1016, 1929, 2113
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
Alw26I GTCTC 1 cut(s) 1146
AlwI GGATC 5 cut(s) 68, 95, 805, 1750, 2457
AoxI GGCC 9 cut(s) 175, 571, 716, 752, 781, 913, 1616, 1698, 1885
ApeKI GCWGC 3 cut(s) 122, 2324, 2335
AseI ATTAAT 1 cut(s) 321
AspS9I GGNCC 6 cut(s) 175, 752, 1606, 1698, 2111, 2289
AsuC2I CCSGG 1 cut(s) 1292
AsuHPI GGTGA 9 cut(s) 647, 728, 764, 863, 895, 1007, 1185, 1429, 1997
AsuII TTCGAA 1 cut(s) 601
AvaII GGWCC 3 cut(s) 1606, 2111, 2289
BaeGI GKGCMC 1 cut(s) 2392
BalI TGGCCA 1 cut(s) 1618
BanI GGYRCC 1 cut(s) 2389
BanII GRGCYC 2 cut(s) 1017, 1058
BbvCI CCTCAGC 1 cut(s) 1365
BbvI GCAGC 3 cut(s) 109, 2336, 2347
BccI CCATC 6 cut(s) 158, 323, 822, 1618, 1825, 2279
BceAI ACGGC 1 cut(s) 739
BciT130I CCWGG 3 cut(s) 1018, 1931, 2115
BciVI GTATCC 2 cut(s) 466, 2081
BclI TGATCA 1 cut(s) 1515
BcnI CCSGG 1 cut(s) 1292
BcoDI GTCTC 1 cut(s) 1146
BcuI ACTAGT 1 cut(s) 1310
BfaI CTAG 4 cut(s) 213, 1311, 1634, 2468
BfmI CTRYAG 3 cut(s) 396, 1601, 2081
BfrI CTTAAG 1 cut(s) 1145
BfuI GTATCC 2 cut(s) 466, 2081
BglII AGATCT 1 cut(s) 1663
BisI GCNGC 3 cut(s) 123, 2325, 2336
BlsI GCNGC 3 cut(s) 124, 2326, 2337
BmcAI AGTACT 1 cut(s) 1738
Bme1390I CCNGG 4 cut(s) 1018, 1292, 1931, 2115
Bme18I GGWCC 3 cut(s) 1606, 2111, 2289
BmgT120I GGNCC 6 cut(s) 175, 752, 1606, 1698, 2111, 2289
BmiI GGNNCC 6 cut(s) 1057, 1288, 2112, 2290, 2291, 2391
BmrFI CCNGG 4 cut(s) 1018, 1292, 1931, 2115
BmsI GCATC 6 cut(s) 382, 538, 898, 1088, 1677, 2459
BpmI CTGGAG 3 cut(s) 680, 711, 1042
Bpu10I CCTNAGC 1 cut(s) 1365
Bpu14I TTCGAA 1 cut(s) 601
BpuEI CTTGAG 6 cut(s) 212, 1678, 2132, 2249, 2266, 2279
BpuMI CCSGG 1 cut(s) 1292
Bsa29I ATCGAT 2 cut(s) 2217, 2493
BsaAI YACGTR 2 cut(s) 1115, 1765
BsaBI GATNNNNATC 1 cut(s) 897
BsaJI CCNNGG 5 cut(s) 129, 1016, 1131, 1290, 2074
BsaWI WCCGGW 1 cut(s) 2552
Bsc4I CCNNNNNNNGG 6 cut(s) 232, 580, 745, 1022, 1883, 2531
Bse1I ACTGG 3 cut(s) 637, 1175, 1461
Bse3DI GCAATG 1 cut(s) 2206
Bse8I GATNNNNATC 1 cut(s) 897
BseBI CCWGG 3 cut(s) 1018, 1931, 2115
BseCI ATCGAT 2 cut(s) 2217, 2493
BseDI CCNNGG 5 cut(s) 129, 1016, 1131, 1290, 2074
BseGI GGATG 4 cut(s) 139, 833, 1125, 1817
BseJI GATNNNNATC 1 cut(s) 897
BseLI CCNNNNNNNGG 6 cut(s) 232, 580, 745, 1022, 1883, 2531
BseMI GCAATG 1 cut(s) 2206
BseMII CTCAG 4 cut(s) 374, 1356, 1737, 2321
BseNI ACTGG 3 cut(s) 637, 1175, 1461
BseRI GAGGAG 1 cut(s) 80
BseSI GKGCMC 1 cut(s) 2392
BseXI GCAGC 3 cut(s) 109, 2336, 2347
BseYI CCCAGC 1 cut(s) 5
BsgI GTGCAG 1 cut(s) 1745
BshFI GGCC 9 cut(s) 177, 573, 718, 754, 783, 915, 1618, 1700, 1887
BshNI GGYRCC 1 cut(s) 2389
BshVI ATCGAT 2 cut(s) 2217, 2493
BsiSI CCGG 2 cut(s) 1292, 2553
BsiWI CGTACG 1 cut(s) 35
BslFI GGGAC 3 cut(s) 665, 1257, 2302
BslI CCNNNNNNNGG 6 cut(s) 232, 580, 745, 1022, 1883, 2531
BsmAI GTCTC 1 cut(s) 1146
BsmFI GGGAC 3 cut(s) 665, 1257, 2302
BsnI GGCC 9 cut(s) 177, 573, 718, 754, 783, 915, 1618, 1700, 1887
Bsp119I TTCGAA 1 cut(s) 601
Bsp1286I GDGCHC 3 cut(s) 1017, 1058, 2392
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 2 cut(s) 844, 1463
BspANI GGCC 9 cut(s) 177, 573, 718, 754, 783, 915, 1618, 1700, 1887
BspCNI CTCAG 4 cut(s) 373, 1357, 1738, 2322
BspDI ATCGAT 2 cut(s) 2217, 2493
BspLI GGNNCC 6 cut(s) 1057, 1288, 2112, 2290, 2291, 2391
BspMAI CTGCAG 1 cut(s) 2085
BspPI GGATC 5 cut(s) 68, 95, 805, 1750, 2457
BspT104I TTCGAA 1 cut(s) 601
BspT107I GGYRCC 1 cut(s) 2389
BspTI CTTAAG 1 cut(s) 1145
BsrDI GCAATG 1 cut(s) 2206
BsrI ACTGG 3 cut(s) 637, 1175, 1461
BssECI CCNNGG 5 cut(s) 129, 1016, 1131, 1290, 2074
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 3 cut(s) 1018, 1931, 2115
Bst4CI ACNGT 8 cut(s) 679, 1380, 1385, 1528, 1563, 1796, 2104, 2485
Bst6I CTCTTC 3 cut(s) 12, 265, 333
BstAFI CTTAAG 1 cut(s) 1145
BstAPI GCANNNNNTGC 1 cut(s) 2321
BstBAI YACGTR 2 cut(s) 1115, 1765
BstBI TTCGAA 1 cut(s) 601
BstC8I GCNNGC 4 cut(s) 846, 913, 1483, 1575
BstDEI CTNAG 6 cut(s) 360, 1365, 1671, 1746, 2012, 2330
BstDSI CCRYGG 3 cut(s) 129, 1131, 2074
BstEII GGTNACC 1 cut(s) 770
BstENI CCTNNNNNAGG 2 cut(s) 578, 1881
BstF5I GGATG 4 cut(s) 139, 833, 1125, 1817
BstMAI GTCTC 1 cut(s) 1146
BstMWI GCNNNNNNNGC 6 cut(s) 174, 943, 1361, 1370, 2233, 2321
BstNI CCWGG 3 cut(s) 1018, 1931, 2115
BstNSI RCATGY 2 cut(s) 763, 1009
BstPI GGTNACC 1 cut(s) 770
BstSCI CCNGG 4 cut(s) 1016, 1290, 1929, 2113
BstSFI CTRYAG 3 cut(s) 396, 1601, 2081
BstSLI GKGCMC 1 cut(s) 2392
BstV1I GCAGC 3 cut(s) 109, 2336, 2347
BstX2I RGATCY 3 cut(s) 797, 1663, 2462
BstXI CCANNNNNNTGG 1 cut(s) 1283
BstYI RGATCY 3 cut(s) 797, 1663, 2462
Bsu15I ATCGAT 2 cut(s) 2217, 2493
BsuI GTATCC 2 cut(s) 466, 2081
BsuRI GGCC 9 cut(s) 177, 573, 718, 754, 783, 915, 1618, 1700, 1887
BsuTUI ATCGAT 2 cut(s) 2217, 2493
BtgI CCRYGG 3 cut(s) 129, 1131, 2074
BtsCI GGATG 4 cut(s) 139, 833, 1125, 1817
BtsIMutI CAGTG 3 cut(s) 145, 2109, 2327
Cac8I GCNNGC 4 cut(s) 846, 913, 1483, 1575
Cfr13I GGNCC 6 cut(s) 175, 752, 1606, 1698, 2111, 2289
ClaI ATCGAT 2 cut(s) 2217, 2493
Csp6I GTAC 9 cut(s) 36, 52, 223, 250, 1213, 1381, 1540, 1737, 2414
CviQI GTAC 9 cut(s) 36, 52, 223, 250, 1213, 1381, 1540, 1737, 2414
DdeI CTNAG 6 cut(s) 360, 1365, 1671, 1746, 2012, 2330
DraI TTTAAA 1 cut(s) 1489
EaeI YGGCCR 2 cut(s) 781, 1616
Eam1104I CTCTTC 3 cut(s) 12, 265, 333
EarI CTCTTC 3 cut(s) 12, 265, 333
Eco130I CCWWGG 1 cut(s) 129
Eco147I AGGCCT 2 cut(s) 573, 1887
Eco24I GRGCYC 2 cut(s) 1017, 1058
Eco47I GGWCC 3 cut(s) 1606, 2111, 2289
Eco57I CTGAAG 3 cut(s) 312, 1761, 1952
Eco91I GGTNACC 1 cut(s) 770
EcoNI CCTNNNNNAGG 2 cut(s) 578, 1881
EcoO109I RGGNCCY 3 cut(s) 175, 1698, 2289
EcoO65I GGTNACC 1 cut(s) 770
EcoRI GAATTC 3 cut(s) 1062, 1906, 2189
EcoRII CCWGG 3 cut(s) 1016, 1929, 2113
EcoT14I CCWWGG 1 cut(s) 129
EcoT22I ATGCAT 2 cut(s) 531, 2452
EcoT38I GRGCYC 2 cut(s) 1017, 1058
ErhI CCWWGG 1 cut(s) 129
FalI AAGNNNNNCTT 2 cut(s) 540, 572
FaqI GGGAC 3 cut(s) 665, 1257, 2302
FauI CCCGC 1 cut(s) 837
FauNDI CATATG 2 cut(s) 198, 1198
FbaI TGATCA 1 cut(s) 1515
Fnu4HI GCNGC 3 cut(s) 123, 2325, 2336
FokI GGATG 4 cut(s) 146, 840, 1112, 1804
FriOI GRGCYC 2 cut(s) 1017, 1058
Fsp4HI GCNGC 3 cut(s) 123, 2325, 2336
FspBI CTAG 4 cut(s) 213, 1311, 1634, 2468
GluI GCNGC 3 cut(s) 123, 2325, 2336
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 3 cut(s) 680, 711, 1042
HaeIII GGCC 9 cut(s) 177, 573, 718, 754, 783, 915, 1618, 1700, 1887
HapII CCGG 2 cut(s) 1292, 2553
HindIII AAGCTT 5 cut(s) 944, 1483, 1847, 2165, 2225
HinfI GANTC 7 cut(s) 664, 854, 966, 977, 1748, 1839, 2428
HpaII CCGG 2 cut(s) 1292, 2553
HphI GGTGA 9 cut(s) 647, 728, 764, 863, 895, 1007, 1185, 1429, 1997
Hpy166II GTNNAC 3 cut(s) 1921, 2078, 2414
Hpy188I TCNGA 7 cut(s) 292, 652, 674, 965, 2284, 2455, 2513
Hpy8I GTNNAC 3 cut(s) 1921, 2078, 2414
HpyCH4III ACNGT 8 cut(s) 679, 1380, 1385, 1528, 1563, 1796, 2104, 2485
HpyCH4IV ACGT 5 cut(s) 1114, 1251, 1764, 2119, 2153
HpyF10VI GCNNNNNNNGC 6 cut(s) 174, 943, 1361, 1370, 2233, 2321
HpyF3I CTNAG 6 cut(s) 360, 1365, 1671, 1746, 2012, 2330
HpySE526I ACGT 5 cut(s) 1114, 1251, 1764, 2119, 2153
KflI GGGWCCC 1 cut(s) 2289
Ksp22I TGATCA 1 cut(s) 1515
LmnI GCTCC 3 cut(s) 1061, 1292, 2555
Lsp1109I GCAGC 3 cut(s) 109, 2336, 2347
LweI GCATC 6 cut(s) 382, 538, 898, 1088, 1677, 2459
MaeI CTAG 4 cut(s) 213, 1311, 1634, 2468
MaeII ACGT 5 cut(s) 1114, 1251, 1764, 2119, 2153
MaeIII GTNAC 7 cut(s) 770, 1191, 1417, 1528, 1712, 2033, 2517
MfeI CAATTG 2 cut(s) 430, 1267
MflI RGATCY 3 cut(s) 797, 1663, 2462
MhlI GDGCHC 3 cut(s) 1017, 1058, 2392
MlsI TGGCCA 1 cut(s) 1618
MluNI TGGCCA 1 cut(s) 1618
MlyI GAGTC 3 cut(s) 986, 1757, 1848
Mox20I TGGCCA 1 cut(s) 1618
Mph1103I ATGCAT 2 cut(s) 531, 2452
MscI TGGCCA 1 cut(s) 1618
MslI CAYNNNNRTG 3 cut(s) 826, 1281, 1941
Msp20I TGGCCA 1 cut(s) 1618
MspA1I CMGCKG 1 cut(s) 389
MspCI CTTAAG 1 cut(s) 1145
MspI CCGG 2 cut(s) 1292, 2553
MspR9I CCNGG 4 cut(s) 1018, 1292, 1931, 2115
MunI CAATTG 2 cut(s) 430, 1267
MvaI CCWGG 3 cut(s) 1018, 1931, 2115
MwoI GCNNNNNNNGC 6 cut(s) 174, 943, 1361, 1370, 2233, 2321
NciI CCSGG 1 cut(s) 1292
NcoI CCATGG 1 cut(s) 129
NdeI CATATG 2 cut(s) 198, 1198
NlaIV GGNNCC 6 cut(s) 1057, 1288, 2112, 2290, 2291, 2391
NmuCI GTSAC 5 cut(s) 770, 1191, 1417, 1528, 2517
NsiI ATGCAT 2 cut(s) 531, 2452
NspI RCATGY 2 cut(s) 763, 1009
NspV TTCGAA 1 cut(s) 601
PceI AGGCCT 2 cut(s) 573, 1887
PciI ACATGT 1 cut(s) 1005
PfeI GAWTC 4 cut(s) 664, 854, 966, 2428
Pfl23II CGTACG 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 2113
PkrI GCNGC 3 cut(s) 124, 2326, 2337
PleI GAGTC 3 cut(s) 985, 1756, 1847
PpsI GAGTC 3 cut(s) 985, 1756, 1847
Ppu21I YACGTR 2 cut(s) 1115, 1765
PpuMI RGGWCCY 1 cut(s) 2289
PscI ACATGT 1 cut(s) 1005
PshBI ATTAAT 1 cut(s) 321
PsiI TTATAA 1 cut(s) 1434
Psp5II RGGWCCY 1 cut(s) 2289
Psp6I CCWGG 3 cut(s) 1016, 1929, 2113
PspEI GGTNACC 1 cut(s) 770
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 3 cut(s) 1016, 1929, 2113
PspLI CGTACG 1 cut(s) 35
PspN4I GGNNCC 6 cut(s) 1057, 1288, 2112, 2290, 2291, 2391
PspPI GGNCC 6 cut(s) 175, 752, 1606, 1698, 2111, 2289
PspPPI RGGWCCY 1 cut(s) 2289
PstI CTGCAG 1 cut(s) 2085
PsuI RGATCY 3 cut(s) 797, 1663, 2462
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 9 cut(s) 37, 53, 224, 251, 1214, 1382, 1541, 1738, 2415
RsaNI GTAC 9 cut(s) 36, 52, 223, 250, 1213, 1381, 1540, 1737, 2414
RseI CAYNNNNRTG 3 cut(s) 826, 1281, 1941
SatI GCNGC 3 cut(s) 123, 2325, 2336
Sau96I GGNCC 6 cut(s) 175, 752, 1606, 1698, 2111, 2289
ScaI AGTACT 1 cut(s) 1738
SchI GAGTC 3 cut(s) 986, 1757, 1848
ScrFI CCNGG 4 cut(s) 1018, 1292, 1931, 2115
SduI GDGCHC 3 cut(s) 1017, 1058, 2392
SfaNI GCATC 6 cut(s) 382, 538, 898, 1088, 1677, 2459
SfcI CTRYAG 3 cut(s) 396, 1601, 2081
SfuI TTCGAA 1 cut(s) 601
SinI GGWCC 3 cut(s) 1606, 2111, 2289
SmiMI CAYNNNNRTG 3 cut(s) 826, 1281, 1941
SmlI CTYRAG 7 cut(s) 191, 1145, 1693, 2147, 2228, 2245, 2294
SmoI CTYRAG 7 cut(s) 191, 1145, 1693, 2147, 2228, 2245, 2294
SpeI ACTAGT 1 cut(s) 1310
SseBI AGGCCT 2 cut(s) 573, 1887
SsiI CCGC 2 cut(s) 844, 1463
SspI AATATT 1 cut(s) 607
SspMI CTAG 4 cut(s) 213, 1311, 1634, 2468
StuI AGGCCT 2 cut(s) 573, 1887
StyD4I CCNGG 4 cut(s) 1016, 1290, 1929, 2113
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 8 cut(s) 679, 1380, 1385, 1528, 1563, 1796, 2104, 2485
TaiI ACGT 5 cut(s) 1117, 1254, 1767, 2122, 2156
TaqI TCGA 7 cut(s) 258, 315, 451, 601, 1939, 2217, 2493
TaqII GACCGA 1 cut(s) 627
TatI WGTACW 4 cut(s) 51, 249, 1380, 1736
TfiI GAWTC 4 cut(s) 664, 854, 966, 2428
TscAI CASTG 3 cut(s) 145, 2109, 2334
TseFI GTSAC 5 cut(s) 770, 1191, 1417, 1528, 2517
TseI GCWGC 3 cut(s) 122, 2324, 2335
Tsp45I GTSAC 5 cut(s) 770, 1191, 1417, 1528, 2517
TspGWI ACGGA 4 cut(s) 53, 558, 1148, 2063
TspRI CASTG 3 cut(s) 145, 2109, 2334
Vha464I CTTAAG 1 cut(s) 1145
VpaK11BI GGWCC 3 cut(s) 1606, 2111, 2289
VspI ATTAAT 1 cut(s) 321
XagI CCTNNNNNAGG 2 cut(s) 578, 1881
XceI RCATGY 2 cut(s) 763, 1009
XcmI CCANNNNNNNNNTGG 1 cut(s) 1403
XspI CTAG 4 cut(s) 213, 1311, 1634, 2468
ZrmI AGTACT 1 cut(s) 1738
Zsp2I ATGCAT 2 cut(s) 531, 2452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.