Rroxscaffold_175G00432220

40S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000175
OG0004553
Physical Location & Seq
Forward (+)
671934 .. 673671
1738 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_175G00432220.1

Sequence Viewer

Length: 426 bp
ATGGGTCGAACCACGAAGACGAGGACTCCGTGCTATGGGTGGTGGAACGGCCAGAAATTTACGGAAAACGCGAAATCGCCGGAGACCCGAAATGCCACTGCGGCTCCGAAGAAGGAAAAGGCACCGCCGCCGTCGTCGAAGCCGGCCAAGTCCGGCGGAGGGAAGCAGAAGAAGAAGAAATGGAGCAAAGGAAAGCAGAAGGAGAAGGTAAACAACATGGTGCTGTTCGACCAGGCCACCTACGATAAGCTGCTGTCTGAGGCCCCCAAGTTCAAGCTGATCACACCGTCCATTCTCTCTGATCGTCTCAGGATCAACGGCTCTCTGGCCAGGAGGGCAATCAAGGACTTGATGGCTAGGGGATCCATCAGGATGGTCTCTGCTCACGCCAGCCAGCAGATCTACACTAGGGCAACCAACACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S25

Protein Analysis

141

Amino Acids

15.72

Weight (kDa)

10.65

Isoelectric Point (pI)

36.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S25 PF03297 40 - 138 9.5e-41 S25 ribosomal protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 121
AccII CGCG 1 cut(s) 71
AciI CCGC 4 cut(s) 101, 125, 128, 156
AclWI GGATC 3 cut(s) 320, 357, 370
AcoI YGGCCR 3 cut(s) 49, 144, 327
AcsI RAATTY 1 cut(s) 56
AfiI CCNNNNNNNGG 2 cut(s) 35, 159
AgsI TTSAA 1 cut(s) 274
AjnI CCWGG 2 cut(s) 231, 329
AluBI AGCT 2 cut(s) 250, 277
AluI AGCT 2 cut(s) 250, 277
Alw26I GTCTC 3 cut(s) 77, 311, 382
AlwI GGATC 3 cut(s) 320, 357, 370
AoxI GGCC 5 cut(s) 49, 144, 234, 261, 327
ApeKI GCWGC 1 cut(s) 250
ApoI RAATTY 1 cut(s) 56
AspS9I GGNCC 1 cut(s) 262
BalI TGGCCA 1 cut(s) 329
BamHI GGATCC 1 cut(s) 362
BanI GGYRCC 1 cut(s) 121
BbsI GAAGAC 1 cut(s) 23
BbvI GCAGC 1 cut(s) 237
BccI CCATC 3 cut(s) 346, 367, 374
BceAI ACGGC 3 cut(s) 64, 115, 334
BciT130I CCWGG 2 cut(s) 233, 331
BclI TGATCA 1 cut(s) 279
BcoDI GTCTC 3 cut(s) 77, 311, 382
BfaI CTAG 3 cut(s) 357, 408, 424
BglI GCCNNNNNGGC 2 cut(s) 101, 335
BglII AGATCT 1 cut(s) 399
BisI GCNGC 3 cut(s) 102, 128, 251
BlsI GCNGC 3 cut(s) 103, 129, 252
Bme1390I CCNGG 2 cut(s) 233, 331
BmgT120I GGNCC 1 cut(s) 262
BmiI GGNNCC 4 cut(s) 105, 123, 264, 364
BmrFI CCNGG 2 cut(s) 233, 331
BpiI GAAGAC 1 cut(s) 23
BsaI GGTCTC 2 cut(s) 77, 382
BsaXI ACNNNNNCTCC 4 cut(s) 10, 40, 88, 118
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 159
Bse118I RCCGGY 1 cut(s) 142
BseBI CCWGG 2 cut(s) 233, 331
BseGI GGATG 1 cut(s) 378
BseLI CCNNNNNNNGG 2 cut(s) 35, 159
BseMII CTCAG 2 cut(s) 249, 322
BseXI GCAGC 1 cut(s) 237
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 5 cut(s) 51, 146, 236, 263, 329
BshNI GGYRCC 1 cut(s) 121
BsiSI CCGG 3 cut(s) 80, 143, 153
BslI CCNNNNNNNGG 2 cut(s) 35, 159
BsmAI GTCTC 3 cut(s) 77, 311, 382
BsmBI CGTCTC 1 cut(s) 311
BsnI GGCC 5 cut(s) 51, 146, 236, 263, 329
Bso31I GGTCTC 2 cut(s) 77, 382
Bsp143I GATC 5 cut(s) 279, 301, 312, 362, 399
BspACI CCGC 4 cut(s) 101, 125, 128, 156
BspANI GGCC 5 cut(s) 51, 146, 236, 263, 329
BspCNI CTCAG 2 cut(s) 250, 321
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 4 cut(s) 105, 123, 264, 364
BspPI GGATC 3 cut(s) 320, 357, 370
BspT107I GGYRCC 1 cut(s) 121
BspTNI GGTCTC 2 cut(s) 77, 382
BsrFI RCCGGY 1 cut(s) 142
BssAI RCCGGY 1 cut(s) 142
BssMI GATC 5 cut(s) 279, 301, 312, 362, 399
Bst2UI CCWGG 2 cut(s) 233, 331
Bst4CI ACNGT 1 cut(s) 288
BstC8I GCNNGC 3 cut(s) 144, 391, 395
BstDEI CTNAG 2 cut(s) 258, 308
BstF5I GGATG 1 cut(s) 378
BstFNI CGCG 1 cut(s) 71
BstKTI GATC 5 cut(s) 282, 304, 315, 365, 402
BstMAI GTCTC 3 cut(s) 77, 311, 382
BstMBI GATC 5 cut(s) 279, 301, 312, 362, 399
BstMWI GCNNNNNNNGC 2 cut(s) 101, 335
BstNI CCWGG 2 cut(s) 233, 331
BstSCI CCNGG 2 cut(s) 231, 329
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 1 cut(s) 237
BstV2I GAAGAC 1 cut(s) 23
BstX2I RGATCY 2 cut(s) 362, 399
BstXI CCANNNNNNTGG 1 cut(s) 373
BstYI RGATCY 2 cut(s) 362, 399
BsuRI GGCC 5 cut(s) 51, 146, 236, 263, 329
BtsCI GGATG 1 cut(s) 378
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 1 cut(s) 96
Cac8I GCNNGC 3 cut(s) 144, 391, 395
Cfr10I RCCGGY 1 cut(s) 142
Cfr13I GGNCC 1 cut(s) 262
CviAII CATG 1 cut(s) 217
DdeI CTNAG 2 cut(s) 258, 308
DpnI GATC 5 cut(s) 281, 303, 314, 364, 401
DpnII GATC 5 cut(s) 279, 301, 312, 362, 399
EaeI YGGCCR 3 cut(s) 49, 144, 327
EciI GGCGGA 1 cut(s) 171
Eco31I GGTCTC 2 cut(s) 77, 382
EcoO109I RGGNCCY 1 cut(s) 262
EcoRII CCWGG 2 cut(s) 231, 329
Esp3I CGTCTC 1 cut(s) 311
FaeI CATG 1 cut(s) 220
FaiI YATR 2 cut(s) 36, 218
FatI CATG 1 cut(s) 216
FbaI TGATCA 1 cut(s) 279
Fnu4HI GCNGC 3 cut(s) 102, 128, 251
FokI GGATG 1 cut(s) 385
Fsp4HI GCNGC 3 cut(s) 102, 128, 251
FspBI CTAG 3 cut(s) 357, 408, 424
GluI GCNGC 3 cut(s) 102, 128, 251
HaeIII GGCC 5 cut(s) 51, 146, 236, 263, 329
HapII CCGG 3 cut(s) 80, 143, 153
Hin1II CATG 1 cut(s) 220
HinfI GANTC 1 cut(s) 25
HpaII CCGG 3 cut(s) 80, 143, 153
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 3 cut(s) 108, 259, 301
Hpy188III TCNNGA 2 cut(s) 310, 370
Hpy8I GTNNAC 1 cut(s) 211
Hpy99I CGWCG 2 cut(s) 136, 139
HpyAV CCTTC 3 cut(s) 106, 193, 199
HpyCH4III ACNGT 1 cut(s) 288
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 335
HpyF3I CTNAG 2 cut(s) 258, 308
Hsp92II CATG 1 cut(s) 220
KroI GCCGGC 1 cut(s) 142
KroNI GCCGGC 1 cut(s) 144
Ksp22I TGATCA 1 cut(s) 279
Kzo9I GATC 5 cut(s) 279, 301, 312, 362, 399
LmnI GCTCC 2 cut(s) 109, 183
Lsp1109I GCAGC 1 cut(s) 237
MaeI CTAG 3 cut(s) 357, 408, 424
MalI GATC 5 cut(s) 281, 303, 314, 364, 401
MboI GATC 5 cut(s) 279, 301, 312, 362, 399
MboII GAAGA 5 cut(s) 28, 121, 181, 184, 187
MflI RGATCY 2 cut(s) 362, 399
MlsI TGGCCA 1 cut(s) 329
MluCI AATT 1 cut(s) 56
MluNI TGGCCA 1 cut(s) 329
MlyI GAGTC 1 cut(s) 19
MnlI CCTC 4 cut(s) 15, 152, 253, 327
Mox20I TGGCCA 1 cut(s) 329
MroNI GCCGGC 1 cut(s) 142
MscI TGGCCA 1 cut(s) 329
MslI CAYNNNNRTG 1 cut(s) 371
Msp20I TGGCCA 1 cut(s) 329
MspI CCGG 3 cut(s) 80, 143, 153
MspR9I CCNGG 2 cut(s) 233, 331
MvaI CCWGG 2 cut(s) 233, 331
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 2 cut(s) 101, 335
NaeI GCCGGC 1 cut(s) 144
NdeII GATC 5 cut(s) 279, 301, 312, 362, 399
NgoMIV GCCGGC 1 cut(s) 142
NlaIII CATG 1 cut(s) 220
NlaIV GGNNCC 4 cut(s) 105, 123, 264, 364
PcsI WCGNNNNNNNCGW 2 cut(s) 26, 68
PdiI GCCGGC 1 cut(s) 144
PkrI GCNGC 3 cut(s) 103, 129, 252
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
Psp6I CCWGG 2 cut(s) 231, 329
PspGI CCWGG 2 cut(s) 231, 329
PspN4I GGNNCC 4 cut(s) 105, 123, 264, 364
PspPI GGNCC 1 cut(s) 262
PsuI RGATCY 2 cut(s) 362, 399
RseI CAYNNNNRTG 1 cut(s) 371
SatI GCNGC 3 cut(s) 102, 128, 251
Sau3AI GATC 5 cut(s) 279, 301, 312, 362, 399
Sau96I GGNCC 1 cut(s) 262
SchI GAGTC 1 cut(s) 19
ScrFI CCNGG 2 cut(s) 233, 331
SetI ASST 5 cut(s) 210, 242, 252, 279, 425
SmiMI CAYNNNNRTG 1 cut(s) 371
Sse9I AATT 1 cut(s) 56
SsiI CCGC 4 cut(s) 101, 125, 128, 156
SspMI CTAG 3 cut(s) 357, 408, 424
StyD4I CCNGG 2 cut(s) 231, 329
TaaI ACNGT 1 cut(s) 288
TaqI TCGA 3 cut(s) 7, 137, 228
TasI AATT 1 cut(s) 56
TauI GCSGC 2 cut(s) 104, 130
TscAI CASTG 1 cut(s) 103
TseI GCWGC 1 cut(s) 250
TspGWI ACGGA 2 cut(s) 18, 77
TspRI CASTG 1 cut(s) 103
XapI RAATTY 1 cut(s) 56
XspI CTAG 3 cut(s) 357, 408, 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.