Rroxscaffold_7G00198690

membrane protein At4g09580-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0004553
Physical Location & Seq
Forward (+)
44342118 .. 44357596
15479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00198690.1

Sequence Viewer

Length: 561 bp
ATGCCCGCCGCCGATTACGGTAAACTCAAGCTTCCACGCACCATTGCCGATCTTCGCTTGCTCAAAGATCATCTTGGGACCTATGCAAACGATTATCCAGCTCAATTAATTCGGGAGATGAAATCCGATGACCAAAATAGCATCCATCGACCAAATTTTCCAACAATCTTAAGAGTAGAACTCGTTGGTAGATGTTTCCTTGTGGAAAGCTTGGACATAAAGATAAGTCTGGTTGCCTATGGCAATTTGATGCCTCGGAAGATTAATGTCCCTACGCGACAAGCGTGGTCCGGGCCTACCATTGTACCGATACTGTCTCAACTTAACCACCCATTAGGTGTTGGGTACTATATAGTGAAGGAGATACTTTATGAGCTAAATGAAGTCTCAAAAGCTAAAAATGATACAAGCGCTAGGAGGCGAGAAGCTGCCCCCCCGAGTCGCTCTTCCTTCACCGGCCGATATCTCTCTCATCCGACCTCCAATCGAGTTGATTCCAAAAGAGATTTTGCTGGGCTTGAGCTATATTACAACTTTGTAGAAGACATCAAGGGGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

186

Amino Acids

21.12

Weight (kDa)

9.49

Isoelectric Point (pI)

36.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 277
AciI CCGC 2 cut(s) 6, 9
AcoI YGGCCR 1 cut(s) 457
AcsI RAATTY 1 cut(s) 154
AfaI GTAC 2 cut(s) 306, 347
AfeI AGCGCT 1 cut(s) 412
AflII CTTAAG 1 cut(s) 169
AluBI AGCT 7 cut(s) 31, 101, 210, 376, 395, 428, 523
AluI AGCT 7 cut(s) 31, 101, 210, 376, 395, 428, 523
Alw26I GTCTC 2 cut(s) 321, 391
Ama87I CYCGRG 1 cut(s) 436
Aor51HI AGCGCT 1 cut(s) 412
AoxI GGCC 2 cut(s) 293, 457
ApeKI GCWGC 1 cut(s) 428
ApoI RAATTY 1 cut(s) 154
AseI ATTAAT 2 cut(s) 107, 264
AspLEI GCGC 1 cut(s) 413
AspS9I GGNCC 3 cut(s) 78, 288, 293
AsuC2I CCSGG 1 cut(s) 292
AsuHPI GGTGA 1 cut(s) 445
AvaI CYCGRG 1 cut(s) 436
AvaII GGWCC 2 cut(s) 78, 288
BaeI ACNNNNGTAYC 2 cut(s) 288, 321
BbsI GAAGAC 1 cut(s) 549
BbvI GCAGC 1 cut(s) 415
BccI CCATC 1 cut(s) 153
BcnI CCSGG 1 cut(s) 292
BcoDI GTCTC 2 cut(s) 321, 391
BfaI CTAG 1 cut(s) 414
BfoI RGCGCY 1 cut(s) 414
BfrI CTTAAG 1 cut(s) 169
BisI GCNGC 2 cut(s) 9, 429
BlsI GCNGC 2 cut(s) 10, 430
Bme1390I CCNGG 1 cut(s) 292
Bme18I GGWCC 2 cut(s) 78, 288
BmeT110I CYCGRG 1 cut(s) 436
BmgT120I GGNCC 3 cut(s) 78, 288, 293
BmiI GGNNCC 1 cut(s) 79
BmrFI CCNGG 1 cut(s) 292
BmsI GCATC 2 cut(s) 150, 240
BpiI GAAGAC 1 cut(s) 549
BplI GAGNNNNNCTC 2 cut(s) 165, 197
BpuEI CTTGAG 2 cut(s) 11, 539
BpuMI CCSGG 1 cut(s) 292
BsaJI CCNNGG 1 cut(s) 254
Bse118I RCCGGY 1 cut(s) 455
Bse3DI GCAATG 1 cut(s) 42
BseDI CCNNGG 1 cut(s) 254
BseGI GGATG 2 cut(s) 141, 472
BseMI GCAATG 1 cut(s) 42
BseX3I CGGCCG 1 cut(s) 457
BseXI GCAGC 1 cut(s) 415
BseYI CCCAGC 1 cut(s) 512
Bsh1236I CGCG 1 cut(s) 277
Bsh1285I CGRYCG 1 cut(s) 460
BshFI GGCC 2 cut(s) 295, 459
BsiEI CGRYCG 1 cut(s) 460
BsiHKCI CYCGRG 1 cut(s) 436
BsiSI CCGG 2 cut(s) 291, 456
BslFI GGGAC 2 cut(s) 91, 254
BsmAI GTCTC 2 cut(s) 321, 391
BsmFI GGGAC 2 cut(s) 91, 254
BsnI GGCC 2 cut(s) 295, 459
BsoBI CYCGRG 1 cut(s) 436
Bsp143I GATC 2 cut(s) 49, 67
BspACI CCGC 2 cut(s) 6, 9
BspANI GGCC 2 cut(s) 295, 459
BspFNI CGCG 1 cut(s) 277
BspLI GGNNCC 1 cut(s) 79
BspQI GCTCTTC 1 cut(s) 451
BspTI CTTAAG 1 cut(s) 169
BsrDI GCAATG 1 cut(s) 42
BsrFI RCCGGY 1 cut(s) 455
BssAI RCCGGY 1 cut(s) 455
BssECI CCNNGG 1 cut(s) 254
BssMI GATC 2 cut(s) 49, 67
Bst4CI ACNGT 2 cut(s) 20, 315
Bst6I CTCTTC 1 cut(s) 451
BstAFI CTTAAG 1 cut(s) 169
BstC8I GCNNGC 2 cut(s) 6, 59
BstF5I GGATG 2 cut(s) 141, 472
BstFNI CGCG 1 cut(s) 277
BstH2I RGCGCY 1 cut(s) 414
BstHHI GCGC 1 cut(s) 413
BstKTI GATC 2 cut(s) 52, 70
BstMAI GTCTC 2 cut(s) 321, 391
BstMBI GATC 2 cut(s) 49, 67
BstMCI CGRYCG 1 cut(s) 460
BstSCI CCNGG 1 cut(s) 290
BstUI CGCG 1 cut(s) 277
BstV1I GCAGC 1 cut(s) 415
BstV2I GAAGAC 1 cut(s) 549
BstZI CGGCCG 1 cut(s) 457
BsuRI GGCC 2 cut(s) 295, 459
BtsCI GGATG 2 cut(s) 141, 472
Cac8I GCNNGC 2 cut(s) 6, 59
CfoI GCGC 1 cut(s) 413
Cfr10I RCCGGY 1 cut(s) 455
Cfr13I GGNCC 3 cut(s) 78, 288, 293
Csp6I GTAC 2 cut(s) 305, 346
CviQI GTAC 2 cut(s) 305, 346
DpnI GATC 2 cut(s) 51, 69
DpnII GATC 2 cut(s) 49, 67
EaeI YGGCCR 1 cut(s) 457
EagI CGGCCG 1 cut(s) 457
Eam1104I CTCTTC 1 cut(s) 451
EarI CTCTTC 1 cut(s) 451
EclXI CGGCCG 1 cut(s) 457
Eco32I GATATC 1 cut(s) 464
Eco47I GGWCC 2 cut(s) 78, 288
Eco47III AGCGCT 1 cut(s) 412
Eco52I CGGCCG 1 cut(s) 457
Eco88I CYCGRG 1 cut(s) 436
EcoO109I RGGNCCY 1 cut(s) 78
EcoRV GATATC 1 cut(s) 464
FaiI YATR 7 cut(s) 84, 218, 240, 351, 353, 372, 526
FalI AAGNNNNNCTT 2 cut(s) 57, 89
FaqI GGGAC 2 cut(s) 91, 254
FauI CCCGC 1 cut(s) 13
Fnu4HI GCNGC 2 cut(s) 9, 429
FokI GGATG 2 cut(s) 128, 459
Fsp4HI GCNGC 2 cut(s) 9, 429
FspBI CTAG 1 cut(s) 414
GlaI GCGC 1 cut(s) 412
GluI GCNGC 2 cut(s) 9, 429
GsaI CCCAGC 1 cut(s) 516
HaeII RGCGCY 1 cut(s) 414
HaeIII GGCC 2 cut(s) 295, 459
HapII CCGG 2 cut(s) 291, 456
HhaI GCGC 1 cut(s) 413
Hin6I GCGC 1 cut(s) 411
HinP1I GCGC 1 cut(s) 411
HindIII AAGCTT 2 cut(s) 29, 208
HinfI GANTC 2 cut(s) 439, 494
HpaII CCGG 2 cut(s) 291, 456
HphI GGTGA 1 cut(s) 445
Hpy166II GTNNAC 1 cut(s) 23
Hpy188I TCNGA 3 cut(s) 127, 258, 477
Hpy188III TCNNGA 1 cut(s) 113
Hpy8I GTNNAC 1 cut(s) 23
HpyAV CCTTC 2 cut(s) 352, 460
HpyCH4III ACNGT 2 cut(s) 20, 315
HpyCH4V TGCA 1 cut(s) 86
HspAI GCGC 1 cut(s) 411
Kzo9I GATC 2 cut(s) 49, 67
LguI GCTCTTC 1 cut(s) 451
LpnPI CCDG 5 cut(s) 111, 215, 304, 469, 498
Lsp1109I GCAGC 1 cut(s) 415
LweI GCATC 2 cut(s) 150, 240
MaeI CTAG 1 cut(s) 414
MalI GATC 2 cut(s) 51, 69
MboI GATC 2 cut(s) 49, 67
MboII GAAGA 4 cut(s) 44, 271, 438, 554
MluCI AATT 4 cut(s) 104, 108, 154, 244
MlyI GAGTC 1 cut(s) 448
MmeI TCCRAC 2 cut(s) 185, 500
MnlI CCTC 3 cut(s) 264, 411, 490
MseI TTAA 4 cut(s) 107, 170, 264, 324
MspCI CTTAAG 1 cut(s) 169
MspI CCGG 2 cut(s) 291, 456
MspR9I CCNGG 1 cut(s) 292
MvnI CGCG 1 cut(s) 277
NciI CCSGG 1 cut(s) 292
NdeII GATC 2 cut(s) 49, 67
NlaIV GGNNCC 1 cut(s) 79
PciSI GCTCTTC 1 cut(s) 451
PcsI WCGNNNNNNNCGW 1 cut(s) 281
PfeI GAWTC 1 cut(s) 494
PkrI GCNGC 2 cut(s) 10, 430
PleI GAGTC 1 cut(s) 447
PpsI GAGTC 1 cut(s) 447
PpuMI RGGWCCY 1 cut(s) 78
PshBI ATTAAT 2 cut(s) 107, 264
Psp5II RGGWCCY 1 cut(s) 78
PspFI CCCAGC 1 cut(s) 512
PspN4I GGNNCC 1 cut(s) 79
PspPI GGNCC 3 cut(s) 78, 288, 293
PspPPI RGGWCCY 1 cut(s) 78
RsaI GTAC 2 cut(s) 306, 347
RsaNI GTAC 2 cut(s) 305, 346
SapI GCTCTTC 1 cut(s) 451
SaqAI TTAA 4 cut(s) 107, 170, 264, 324
SatI GCNGC 2 cut(s) 9, 429
Sau3AI GATC 2 cut(s) 49, 67
Sau96I GGNCC 3 cut(s) 78, 288, 293
SchI GAGTC 1 cut(s) 448
ScrFI CCNGG 1 cut(s) 292
SfaNI GCATC 2 cut(s) 150, 240
SinI GGWCC 2 cut(s) 78, 288
SmlI CTYRAG 3 cut(s) 26, 169, 518
SmoI CTYRAG 3 cut(s) 26, 169, 518
Sse9I AATT 4 cut(s) 104, 108, 154, 244
SsiI CCGC 2 cut(s) 6, 9
SspMI CTAG 1 cut(s) 414
StyD4I CCNGG 1 cut(s) 290
TaaI ACNGT 2 cut(s) 20, 315
TaqI TCGA 2 cut(s) 148, 487
TasI AATT 4 cut(s) 104, 108, 154, 244
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 1 cut(s) 494
Tru1I TTAA 4 cut(s) 107, 170, 264, 324
Tru9I TTAA 4 cut(s) 107, 170, 264, 324
TseI GCWGC 1 cut(s) 428
TspDTI ATGAA 2 cut(s) 134, 396
Vha464I CTTAAG 1 cut(s) 169
VpaK11BI GGWCC 2 cut(s) 78, 288
VspI ATTAAT 2 cut(s) 107, 264
XapI RAATTY 1 cut(s) 154
XspI CTAG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.