Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0000711

Physical Location & Seq

Genomic Coordinates Reverse (-)

58147522 .. 58149217

Gene Structure 1696 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00264530.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1377 bp

ATGGAAGACCTTACATTATGCGTCTTGCCCTCTCGGCTGGTATTGGAGGCCTCCTTTTCGATTGGATACGATACAGGTGTGATTTCTGGGGCATTGCTCTACATACGTGAGGACTTCAGTGACGTAGACAAGATGACATGGCTGCAGGAGACCATTGTGAGTATGGCAGTTGCCGGAGCCATATTTGGTGCTGCAATTGGAGGGTGGATGAACGATGCTCTTGGCCGGAGAAAGTCAATCTTGTGTGCAGATTTTGTGTTCTTCTTTGGTGCAATAGTCATGGCTTTTGCACCAGCTCCGTGGTTGATCATAATTGGAAGAATTATGGTTGGTTTGGGAGTGGGTATGGCTTCTATGACCTCACCACTATACATCTCAGAAGCTTCTCCTGCTAGTGTCCGAGGTGCTCTTGTTAGTACAAACGGCTTTCTAATTACTGGAGGTCAATTTTTGGCATACCTCATCAATCTTGCATTTACCAAGACTCCTGGAACCTGGCGTTGGATGCTTGGAGTAGCGGGAGTTCCTGCTTTGGTTCAGTTAGGATTGATGTGGTCACTCCTAGAGTCTTCAAGGTGGCTCTACAGAAAGAATAAGGCTGAGGAAGGAGATGTTGGTGATGGCATCATAGCAAAACTAATGGGTGCATTCAGCAACCCTGTGTTTGCTGGATTTGCTTCCAACCAAACGGCTTTAGCACTTCGTCTCATCACTTTTGGTCTCAATGTTGTTGGCACTGTAATCAGTATGTGCTTTGTGGATAGATATGGTTGGAGAAGGCTTATGATCATTTCTATGGTTGGAATTATCACTTGCCTGGTGGTGTTATCTGTGGCGTTTTTCCAGGCGGCAGCTCACGCTCCTCGTATTATGTCACGGCTTATATCGGGAGCTTGCGTGGCTTCGAATGAGGGCTTGAAAAAGGCATGTGGGGGAGAACATCGTGTATGCTACACAAAAGGGTGTCCAAGCAAAGTTGGATTCTTTGCAGTAATCCTTTTAGGATTGTACATCATTATCTACGCACCTGGGATGGGAACCGTACCGTGGCCGGATTGTGAAATCAGAGATACACCCTTTGAGATAGGCACATATGGAGGGATTGCTGCAGTGGCTAATTGGAGTGCCAATCTGATAGTGAGTGAGACATTTTTAACCCTCGCTCATGCTTTGGGTTCTGTTGGCACGTTCCTCCTCTTTGCTGGATTTTCTCTCATTGGACTCATATTTATTTTCTTCTTGGTTCCTGAAACCAAAGGGCTGCAATTTGAGGAGGTTGGCAATTTGCTTAAGAAAGGCTACATACCCAAGTTATTTGCTTCTAAGGCTAAGGAAACCAAGGAAGTCAAGACTGTCGATCATCTTGAGTTGGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005351 GO:0005355 GO:0005402 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0008643 GO:0008645 GO:0015075 GO:0015077 GO:0015078 GO:0015144 GO:0015145 GO:0015149 GO:0015291 GO:0015293 GO:0015294 GO:0015295 GO:0015318 GO:0015672 GO:0015749 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0034219 GO:0034220 GO:0044464 GO:0046323 GO:0051119 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071944 GO:0098655 GO:0098660 GO:0098662 GO:1902600 GO:1904659

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sugar_tr

Protein Analysis

458

Amino Acids

49.28

Weight (kDa)

8.3

Isoelectric Point (pI)

28.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sugar_tr	PF00083	17 - 203	1.9e-50	Sugar (and other) transporter
MFS_1	PF07690	17 - 285	1.2e-18	Major Facilitator Superfamily
Sugar_tr	PF00083	322 - 428	6.7e-17	Sugar (and other) transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000711)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35740 AT4G16480 AT4G16480 AT4G16480
fragaria_vesca	FvH4_3g00750 FvH4_3g00750 FvH4_3g00750 FvH4_3g00750 FvH4_3g00750 FvH4_3g00750 FvH4_3g00750 FvH4_3g00750 FvH4_3g00750 FvH4_3g00750 FvH4_3g00760
malus_domestica	MD05G1108700.v1.1 MD05G1359700.v1.1 MD05G1359900.v1.1 MD10G1335300.v1.1 MD10G1335500.v1.1
prunus_persica	Prupe.4G008300_v2.0.a1 Prupe.4G008300_v2.0.a1 Prupe.4G008400_v2.0.a1
pyrus_communis	pycom05g32480 pycom05g32490 pycom10g28510
rosa_chinensis	RchiOBHm_Chr1g0370501 RchiOBHm_Chr4g0424641 RchiOBHm_Chr5g0000981 RchiOBHm_Chr5g0001001 RchiOBHm_Chr7g0190531
rosa_laevigata	RLG00000004519 RLG00000030911 RLG00000030912
rosa_multiflora	Rmu_co8357507.1_g000001 Rmu_co8503263.1_g000001 Rmu_sc0000255.1_g000023 Rmu_sc0000255.1_g000024 Rmu_sc0002558.1_g000005 Rmu_sc0007329.1_g000007
rosa_roxburghii	Rroxscaffold_1G00075280 Rroxscaffold_1G00075290 Rroxscaffold_3G00264530
rosa_rugosa	Rorug04G0198700 Rorug04G0388100 Rorug04G0388200 Rorug04G0388300 Rorug04G0388400 Rorug04G0388500 Rorug04G0388600 Rorug04G0388700 Rorug04G0388800
rosa_samantha	Rh1AG366200 Rh1BG329000 Rh1CG342600 Rh1DG359300 Rh1DG360000 Rh4AG255600 Rh4BG261000 Rh4CG272100 Rh4DG255600 Rh5AG007800 Rh5AG007900 Rh5BG010000 Rh5BG010100 Rh5CG008500 Rh5CG008600 Rh5DG008500 Rh5DG008600
rosa_wichuraiana	Rw1G032250 Rw5G000810 Rw5G000820 Rw7G009720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	126
AciI	CCGC	2 cut(s)	518, 848
AcoI	YGGCCR	2 cut(s)	223, 1049
AcuI	CTGAAG	1 cut(s)	100
AfaI	GTAC	3 cut(s)	418, 1010, 1044
AfiI	CCNNNNNNNGG	3 cut(s)	501, 1034, 1047
AflII	CTTAAG	1 cut(s)	1289
AgsI	TTSAA	2 cut(s)	573, 919
AjnI	CCWGG	5 cut(s)	487, 494, 816, 843, 1027
AjuI	GAANNNNNNNTTGG	4 cut(s)	484, 516, 597, 629
AluBI	AGCT	4 cut(s)	296, 383, 854, 893
AluI	AGCT	4 cut(s)	296, 383, 854, 893
Alw21I	GWGCWC	1 cut(s)	409
Alw26I	GTCTC	4 cut(s)	143, 710, 725, 1139
AoxI	GGCC	3 cut(s)	48, 223, 1049
ApeKI	GCWGC	5 cut(s)	142, 191, 851, 1106, 1261
AsuHPI	GGTGA	2 cut(s)	354, 629
AsuII	TTCGAA	1 cut(s)	905
BaeI	ACNNNNGTAYC	2 cut(s)	63, 96
BbsI	GAAGAC	2 cut(s)	12, 561
Bbv12I	GWGCWC	1 cut(s)	409
BbvCI	CCTCAGC	1 cut(s)	600
BbvI	GCAGC	5 cut(s)	129, 178, 863, 1093, 1248
BccI	CCATC	2 cut(s)	614, 1027
BceAI	ACGGC	3 cut(s)	439, 705, 893
BciT130I	CCWGG	5 cut(s)	489, 496, 818, 845, 1029
BciVI	GTATCC	1 cut(s)	59
BclI	TGATCA	2 cut(s)	306, 786
BcoDI	GTCTC	4 cut(s)	143, 710, 725, 1139
BfaI	CTAG	2 cut(s)	393, 563
BfmI	CTRYAG	3 cut(s)	143, 583, 1107
BfrI	CTTAAG	1 cut(s)	1289
BfuI	GTATCC	1 cut(s)	59
BglI	GCCNNNNNGGC	1 cut(s)	34
BisI	GCNGC	6 cut(s)	143, 192, 849, 852, 1107, 1262
BlsI	GCNGC	6 cut(s)	144, 193, 850, 853, 1108, 1263
Bme1390I	CCNGG	5 cut(s)	489, 496, 818, 845, 1029
BmiI	GGNNCC	4 cut(s)	178, 493, 1039, 1245
BmrFI	CCNGG	5 cut(s)	489, 496, 818, 845, 1029
BmsI	GCATC	3 cut(s)	205, 495, 633
BpiI	GAAGAC	2 cut(s)	12, 561
BpmI	CTGGAG	1 cut(s)	459
Bpu10I	CCTNAGC	2 cut(s)	600, 1329
Bpu14I	TTCGAA	1 cut(s)	905
BsaAI	YACGTR	1 cut(s)	107
BsaI	GGTCTC	2 cut(s)	143, 725
BsaJI	CCNNGG	5 cut(s)	299, 400, 1028, 1046, 1338
BsaXI	ACNNNNNCTCC	4 cut(s)	469, 499, 882, 912
Bsc4I	CCNNNNNNNGG	3 cut(s)	501, 1034, 1047
Bse1I	ACTGG	1 cut(s)	442
Bse3DI	GCAATG	1 cut(s)	92
BseBI	CCWGG	5 cut(s)	489, 496, 818, 845, 1029
BseDI	CCNNGG	5 cut(s)	299, 400, 1028, 1046, 1338
BseGI	GGATG	3 cut(s)	213, 510, 1038
BseLI	CCNNNNNNNGG	3 cut(s)	501, 1034, 1047
BseMI	GCAATG	1 cut(s)	92
BseMII	CTCAG	2 cut(s)	390, 591
BseNI	ACTGG	1 cut(s)	442
BseRI	GAGGAG	3 cut(s)	852, 1184, 1286
BseXI	GCAGC	5 cut(s)	129, 178, 863, 1093, 1248
BsgI	GTGCAG	1 cut(s)	267
BshFI	GGCC	3 cut(s)	50, 225, 1051
BsiHKAI	GWGCWC	1 cut(s)	409
BsiSI	CCGG	3 cut(s)	174, 226, 1052
BslI	CCNNNNNNNGG	3 cut(s)	501, 1034, 1047
BsmAI	GTCTC	4 cut(s)	143, 710, 725, 1139
BsmBI	CGTCTC	1 cut(s)	710
BsmI	GAATGC	1 cut(s)	647
BsnI	GGCC	3 cut(s)	50, 225, 1051
Bso31I	GGTCTC	2 cut(s)	143, 725
Bsp119I	TTCGAA	1 cut(s)	905
Bsp1286I	GDGCHC	1 cut(s)	409
Bsp1407I	TGTACA	1 cut(s)	1008
Bsp143I	GATC	3 cut(s)	306, 786, 1357
BspACI	CCGC	2 cut(s)	518, 848
BspANI	GGCC	3 cut(s)	50, 225, 1051
BspCNI	CTCAG	2 cut(s)	389, 592
BspLI	GGNNCC	4 cut(s)	178, 493, 1039, 1245
BspMAI	CTGCAG	2 cut(s)	147, 1111
BspT104I	TTCGAA	1 cut(s)	905
BspTI	CTTAAG	1 cut(s)	1289
BspTNI	GGTCTC	2 cut(s)	143, 725
BsrDI	GCAATG	1 cut(s)	92
BsrGI	TGTACA	1 cut(s)	1008
BsrI	ACTGG	1 cut(s)	442
BssECI	CCNNGG	5 cut(s)	299, 400, 1028, 1046, 1338
BssMI	GATC	3 cut(s)	306, 786, 1357
BssT1I	CCWWGG	1 cut(s)	1338
Bst2UI	CCWGG	5 cut(s)	489, 496, 818, 845, 1029
Bst4CI	ACNGT	4 cut(s)	739, 1042, 1047, 1354
BstAFI	CTTAAG	1 cut(s)	1289
BstAUI	TGTACA	1 cut(s)	1008
BstBAI	YACGTR	1 cut(s)	107
BstBI	TTCGAA	1 cut(s)	905
BstC8I	GCNNGC	1 cut(s)	895
BstDEI	CTNAG	4 cut(s)	376, 600, 1323, 1329
BstDSI	CCRYGG	2 cut(s)	299, 1046
BstF5I	GGATG	3 cut(s)	213, 510, 1038
BstKTI	GATC	3 cut(s)	309, 789, 1360
BstMAI	GTCTC	4 cut(s)	143, 710, 725, 1139
BstMBI	GATC	3 cut(s)	306, 786, 1357
BstMWI	GCNNNNNNNGC	8 cut(s)	34, 389, 505, 674, 857, 899, 1112, 1325
BstNI	CCWGG	5 cut(s)	489, 496, 818, 845, 1029
BstNSI	RCATGY	1 cut(s)	930
BstSCI	CCNGG	5 cut(s)	487, 494, 816, 843, 1027
BstSFI	CTRYAG	3 cut(s)	143, 583, 1107
BstV1I	GCAGC	5 cut(s)	129, 178, 863, 1093, 1248
BstV2I	GAAGAC	2 cut(s)	12, 561
BstXI	CCANNNNNNTGG	1 cut(s)	300
BsuI	GTATCC	1 cut(s)	59
BsuRI	GGCC	3 cut(s)	50, 225, 1051
BtgI	CCRYGG	2 cut(s)	299, 1046
BtsCI	GGATG	3 cut(s)	213, 510, 1038
BtsI	GCAGTG	1 cut(s)	1116
BtsIMutI	CAGTG	3 cut(s)	124, 735, 1116
Cac8I	GCNNGC	1 cut(s)	895
CseI	GACGC	1 cut(s)	10
Csp6I	GTAC	3 cut(s)	417, 1009, 1043
CviAII	CATG	4 cut(s)	138, 280, 927, 1166
CviQI	GTAC	3 cut(s)	417, 1009, 1043
DdeI	CTNAG	4 cut(s)	376, 600, 1323, 1329
DpnI	GATC	3 cut(s)	308, 788, 1359
DpnII	GATC	3 cut(s)	306, 786, 1357
EaeI	YGGCCR	2 cut(s)	223, 1049
Eco130I	CCWWGG	1 cut(s)	1338
Eco147I	AGGCCT	1 cut(s)	50
Eco31I	GGTCTC	2 cut(s)	143, 725
Eco57I	CTGAAG	1 cut(s)	100
EcoRII	CCWGG	5 cut(s)	487, 494, 816, 843, 1027
EcoT14I	CCWWGG	1 cut(s)	1338
ErhI	CCWWGG	1 cut(s)	1338
Esp3I	CGTCTC	1 cut(s)	710
FaeI	CATG	4 cut(s)	141, 283, 930, 1169
FalI	AAGNNNNNCTT	2 cut(s)	224, 256
FatI	CATG	4 cut(s)	137, 279, 926, 1165
FauI	CCCGC	1 cut(s)	511
FauNDI	CATATG	1 cut(s)	1093
FbaI	TGATCA	2 cut(s)	306, 786
FblI	GTMKAC	1 cut(s)	126
Fnu4HI	GCNGC	6 cut(s)	143, 192, 849, 852, 1107, 1262
FokI	GGATG	3 cut(s)	220, 517, 1045
Fsp4HI	GCNGC	6 cut(s)	143, 192, 849, 852, 1107, 1262
FspBI	CTAG	2 cut(s)	393, 563
GluI	GCNGC	6 cut(s)	143, 192, 849, 852, 1107, 1262
GsuI	CTGGAG	1 cut(s)	459
HaeIII	GGCC	3 cut(s)	50, 225, 1051
HapII	CCGG	3 cut(s)	174, 226, 1052
HgaI	GACGC	1 cut(s)	10
Hin1II	CATG	4 cut(s)	141, 283, 930, 1169
HindIII	AAGCTT	1 cut(s)	381
HinfI	GANTC	4 cut(s)	484, 566, 981, 1221
HpaII	CCGG	3 cut(s)	174, 226, 1052
HphI	GGTGA	2 cut(s)	354, 629
Hpy166II	GTNNAC	1 cut(s)	127
Hpy188I	TCNGA	5 cut(s)	379, 401, 1067, 1134, 1376
Hpy188III	TCNNGA	4 cut(s)	888, 1247, 1348, 1364
Hpy8I	GTNNAC	1 cut(s)	127
HpyAV	CCTTC	2 cut(s)	599, 771
HpyCH4III	ACNGT	4 cut(s)	739, 1042, 1047, 1354
HpyCH4IV	ACGT	3 cut(s)	106, 123, 1187
HpyF10VI	GCNNNNNNNGC	8 cut(s)	34, 389, 505, 674, 857, 899, 1112, 1325
HpyF3I	CTNAG	4 cut(s)	376, 600, 1323, 1329
HpySE526I	ACGT	3 cut(s)	106, 123, 1187
Hsp92II	CATG	4 cut(s)	141, 283, 930, 1169
Ksp22I	TGATCA	2 cut(s)	306, 786
Kzo9I	GATC	3 cut(s)	306, 786, 1357
LmnI	GCTCC	4 cut(s)	176, 301, 865, 890
Lsp1109I	GCAGC	5 cut(s)	129, 178, 863, 1093, 1248
LweI	GCATC	3 cut(s)	205, 495, 633
MaeI	CTAG	2 cut(s)	393, 563
MaeII	ACGT	3 cut(s)	106, 123, 1187
MaeIII	GTNAC	3 cut(s)	119, 555, 873
MalI	GATC	3 cut(s)	308, 788, 1359
MboI	GATC	3 cut(s)	306, 786, 1357
MboII	GAAGA	5 cut(s)	17, 253, 330, 561, 1228
MfeI	CAATTG	1 cut(s)	195
MhlI	GDGCHC	1 cut(s)	409
MluCI	AATT	9 cut(s)	195, 312, 321, 432, 446, 804, 1117, 1265, 1282
MlyI	GAGTC	3 cut(s)	478, 575, 1215
MmeI	TCCRAC	5 cut(s)	482, 705, 752, 781, 958
MseI	TTAA	2 cut(s)	1154, 1290
MslI	CAYNNNNRTG	1 cut(s)	794
MspCI	CTTAAG	1 cut(s)	1289
MspI	CCGG	3 cut(s)	174, 226, 1052
MspR9I	CCNGG	5 cut(s)	489, 496, 818, 845, 1029
MunI	CAATTG	1 cut(s)	195
Mva1269I	GAATGC	1 cut(s)	647
MvaI	CCWGG	5 cut(s)	489, 496, 818, 845, 1029
MwoI	GCNNNNNNNGC	8 cut(s)	34, 389, 505, 674, 857, 899, 1112, 1325
NdeI	CATATG	1 cut(s)	1093
NdeII	GATC	3 cut(s)	306, 786, 1357
NlaIII	CATG	4 cut(s)	141, 283, 930, 1169
NlaIV	GGNNCC	4 cut(s)	178, 493, 1039, 1245
NmeAIII	GCCGAG	1 cut(s)	13
NmuCI	GTSAC	3 cut(s)	119, 555, 873
NspI	RCATGY	1 cut(s)	930
NspV	TTCGAA	1 cut(s)	905
PceI	AGGCCT	1 cut(s)	50
PctI	GAATGC	1 cut(s)	647
PfeI	GAWTC	1 cut(s)	981
PfoI	TCCNGGA	1 cut(s)	487
PkrI	GCNGC	6 cut(s)	144, 193, 850, 853, 1108, 1263
PleI	GAGTC	3 cut(s)	478, 574, 1215
PpsI	GAGTC	3 cut(s)	478, 574, 1215
Ppu21I	YACGTR	1 cut(s)	107
Psp6I	CCWGG	5 cut(s)	487, 494, 816, 843, 1027
PspGI	CCWGG	5 cut(s)	487, 494, 816, 843, 1027
PspN4I	GGNNCC	4 cut(s)	178, 493, 1039, 1245
PsrI	GAACNNNNNNTAC	2 cut(s)	507, 539
PstI	CTGCAG	2 cut(s)	147, 1111
RsaI	GTAC	3 cut(s)	418, 1010, 1044
RsaNI	GTAC	3 cut(s)	417, 1009, 1043
RseI	CAYNNNNRTG	1 cut(s)	794
SaqAI	TTAA	2 cut(s)	1154, 1290
SatI	GCNGC	6 cut(s)	143, 192, 849, 852, 1107, 1262
Sau3AI	GATC	3 cut(s)	306, 786, 1357
SchI	GAGTC	3 cut(s)	478, 575, 1215
ScrFI	CCNGG	5 cut(s)	489, 496, 818, 845, 1029
SduI	GDGCHC	1 cut(s)	409
SfaNI	GCATC	3 cut(s)	205, 495, 633
SfcI	CTRYAG	3 cut(s)	143, 583, 1107
SfuI	TTCGAA	1 cut(s)	905
SmiMI	CAYNNNNRTG	1 cut(s)	794
SmlI	CTYRAG	2 cut(s)	1289, 1364
SmoI	CTYRAG	2 cut(s)	1289, 1364
Sse9I	AATT	9 cut(s)	195, 312, 321, 432, 446, 804, 1117, 1265, 1282
SseBI	AGGCCT	1 cut(s)	50
SsiI	CCGC	2 cut(s)	518, 848
SspMI	CTAG	2 cut(s)	393, 563
StuI	AGGCCT	1 cut(s)	50
StyD4I	CCNGG	5 cut(s)	487, 494, 816, 843, 1027
StyI	CCWWGG	1 cut(s)	1338
TaaI	ACNGT	4 cut(s)	739, 1042, 1047, 1354
TaiI	ACGT	3 cut(s)	109, 126, 1190
TaqI	TCGA	3 cut(s)	59, 905, 1356
TasI	AATT	9 cut(s)	195, 312, 321, 432, 446, 804, 1117, 1265, 1282
TatI	WGTACW	2 cut(s)	416, 1008
TauI	GCSGC	1 cut(s)	851
TfiI	GAWTC	1 cut(s)	981
Tru1I	TTAA	2 cut(s)	1154, 1290
Tru9I	TTAA	2 cut(s)	1154, 1290
TscAI	CASTG	3 cut(s)	124, 742, 1116
TseFI	GTSAC	3 cut(s)	119, 555, 873
TseI	GCWGC	5 cut(s)	142, 191, 851, 1106, 1261
Tsp45I	GTSAC	3 cut(s)	119, 555, 873
TspDTI	ATGAA	1 cut(s)	224
TspGWI	ACGGA	1 cut(s)	288
TspRI	CASTG	3 cut(s)	124, 742, 1116
Vha464I	CTTAAG	1 cut(s)	1289
XceI	RCATGY	1 cut(s)	930
XcmI	CCANNNNNNNNNTGG	1 cut(s)	160
XmiI	GTMKAC	1 cut(s)	126
XspI	CTAG	2 cut(s)	393, 563

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.