Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0002283

Physical Location & Seq

Genomic Coordinates Reverse (-)

65916501 .. 65918817

Gene Structure 2317 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0478600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2127 bp

ATGGTGCAAATTATTCCACAAAGTGTGGACAAACTATGGAACGAATGGGACGTCCGGGTAATGGTGCTAAGTAGTCTCTGCTTACAAATAGTCCTAATCCTCTTGGGAAGCCGGCGAAAGTTCAGTACTCAAAGCTGGGTGAGAATCCTCACATGGACGGCCTACTTATCGGCAGATTGGTTCGCTACTGTCGCGCTTGGAATGATCTCCACATTCCAAGGAGATGGTCTGCAGAACTCACTAACTCCAAACAGCGCTCAAGGAAGTTCCTCAAATCCAAACAGCCCGGCGATCATGGCTTTTTGGGCTCCGTTTCTTCTGGTGCACCTTGGTGGCCCGGACACCATCACTGCTTACTCGTTGGAGGACAACGAGCTGTGGCTAAGGCACCTCTTGGGTTTACTTGTTCAGGTTAGTGCTGCTTTATACATCTTTCTAAGATCATGGACATCAAGCTCCTTACCCCTCTCACTGCTAGCAATTCCAATGTTTGTTGTCGGAATCATCAAATATGGAGAGAGGACTTGGGCCCTCTGGTCTGCTAGCAGTGAGAGACTTAGGGAATCTCTGCCCCCTATTACTGCAGCTCTGACTCCCTTTAAAAATGAGAATCATGAGAGTAGTGGTCGTGTTAGACTAAACTATGCTGCTGCTATTCGTAAAGCCACACATATCCAAGAAGGTCATGCATTATTCAAGTTGTTCAGGCATTTTTACACAGATCAAATCATTGAACAAAAGGATGTTCAACTTTCCGATACTATGATTCAGGACCATTCATTTGGAGATGTCTTCAAGCTAATTGAGATTGAGATTGGTTTTATGTATGATGTGCTGTACACCAAGGCAATCGTAGTGCATTCTCTCACGGGGGTCATGTTCCGAGCCATCTCGCTGGTTTGCTACATTTCCACATTTACGGTCTTTTGTTTCATCATTGATAAGAAGATTTATTTGACCATTGATGTAGATTTGACTTACTTCTTGTGTGCTGGAGCAATTGCACTTGAGATCTATGCCATCTTTGTACAAGTTTCTACAGATTGGACATTGTACTGGTTAAGCAAGCAAGAGCGACCACTGGCGTATACTATCTACCAAGCAATCTCTTCATTTCGATCAAATCTGAGCCTTGAAAAGAGGTGGTCTGAATCCATGGGCCAATTCAATGTCATAGAGTTTTGTTTCAAATTGAAACCGCCAAAGTGTACTGGAATTCCTAGGTTCCTGGCTATCTACGAGATGTATTCTAAACATCAGCACACAGTTTTGGAGGGCGTACCAATCAAAATGAAACAACTTATCTTTGAGCACCTTCAAGATGCTAAATTAGTTGTTGATAATGCAGAGCAGAATTCCAAATTTCAAGTACGTAGTCAAGTCTTGACTTGGAGAGGTGAACATGCGGTAGAAAAGAGTGGATGTACTGACAAATATGTCAAACATTGTGCCGCGAATGAAGTACCGTTTGAAACCAGCATTGCTTTGTGGCATGTAGCAACAGATATATGCTTCTATAATGATGTTGACAAAAATCAAAAGGCCTCTGATCCTAATTCCAAATCCAAGGTGAGCAAGTTGCTGTCAGATTATATGATGTATATTCTTGCCATCTGCCCTTTCATGTTGCCCCAAGGGATTGGACAGATTTTGTTTCGACAAATATGCGAGTCTGCCACAACATTTTTTGATGAGAGAAGGGAAACCATAAGTAAGACAGAGCGATTGGTTGGAAAAAGTGCCAGTCAGGTGTTGTACGAGATGAAGTCAGAGTTGAATCCATGGCTAGATGAGTTTGAGTCCGGAGATGGAGGAAAACTAAACAATGTAGTGTTGCTTGAAGGGTGTGTGCTTGCACATAAGTTGCAGGCCCTGGAAACGGAGGAGAGCTGGACTAATGAAGATAAATGGGGAATGATAAGTCGAGTGTGGATAGAAATGTTGTGTTATGCAGCAAGCCAATGTGGATGGACGGAGCACGGTCAACAACTTAGGCGTGGCGGAGAGCTTCTTACTCATGTCTCCCTTCTCATGGCAAATCTTGGTATCGGTGAACAGAGTAAGATATTGAGTGAGCAGGACATGGACCGGTTACAGGATTTAAAAAACTTGTGTGAAAAGTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004611 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0008150 GO:0008152 GO:0008964 GO:0009060 GO:0009987 GO:0015980 GO:0016829 GO:0016830 GO:0016831 GO:0016999 GO:0017144 GO:0019752 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045333 GO:0055114 GO:0071704 GO:0072350

KEGG Pathways

Metabolic & Signaling

ko00620 ko00680 ko00710 ko00720 ko01100 ko01120 ko01200 map00620 map00680 map00710 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

PEPcase

Protein Analysis

708

Amino Acids

80.68

Weight (kDa)

6.06

Isoelectric Point (pI)

41.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF4220	PF13968	52 - 393	7.3e-94	Domain of unknown function (DUF4220)
DUF594	PF04578	631 - 683	3.1e-21	Protein of unknown function, DUF594

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002283)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr1g0314571 RchiOBHm_Chr1g0333411 RchiOBHm_Chr1g0341171 RchiOBHm_Chr1g0355601 RchiOBHm_Chr1g0362661 RchiOBHm_Chr2g0135871 RchiOBHm_Chr2g0155311 RchiOBHm_Chr4g0426971 RchiOBHm_Chr4g0434611 RchiOBHm_Chr5g0016171 RchiOBHm_Chr5g0016231 RchiOBHm_Chr6g0248021 RchiOBHm_Chr6g0302761
rosa_laevigata	RLG00000002685 RLG00000008332 RLG00000009004 RLG00000018585 RLG00000023270 RLG00000025198
rosa_multiflora	Rmu_sc0001376.1_g000006 Rmu_sc0002968.1_g000005
rosa_roxburghii	Rroxscaffold_3G00219200
rosa_rugosa	Rorug01G0313400 Rorug05G0478600
rosa_samantha	Rh1CG246800 Rh2AG443800 Rh2AG514200 Rh2AG551000 Rh2BG052200 Rh2CG430800 Rh2CG499100 Rh4AG183700 Rh4CG195700 Rh4DG179000 Rh5CG135500 Rh5CG135700 Rh5DG275400 Rh5DG564200 Rh6BG040300 Rh7DG279100
rosa_wichuraiana	Rw2G011220 Rw4G022300 Rw7G012030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1436
AatII	GACGTC	1 cut(s)	54
AccB1I	GGYRCC	1 cut(s)	387
AccI	GTMKAC	1 cut(s)	1088
AccII	CGCG	2 cut(s)	194, 1454
AccIII	TCCGGA	1 cut(s)	1802
AciI	CCGC	4 cut(s)	1199, 1406, 1452, 2001
AclWI	GGATC	1 cut(s)	1544
AcsI	RAATTY	3 cut(s)	1215, 1354, 1361
AcyI	GRCGYC	1 cut(s)	51
AdeI	CACNNNGTG	1 cut(s)	23
AfeI	AGCGCT	1 cut(s)	256
AfiI	CCNNNNNNNGG	4 cut(s)	61, 1879, 2032, 2095
AgeI	ACCGGT	1 cut(s)	2088
AjnI	CCWGG	2 cut(s)	1227, 1872
AleI	CACNNNNGTG	1 cut(s)	330
AluBI	AGCT	7 cut(s)	135, 376, 456, 587, 799, 1890, 2008
AluI	AGCT	7 cut(s)	135, 376, 456, 587, 799, 1890, 2008
Alw21I	GWGCWC	3 cut(s)	327, 1314, 1980
Alw26I	GTCTC	3 cut(s)	80, 547, 2026
Alw44I	GTGCAC	1 cut(s)	323
AlwI	GGATC	1 cut(s)	1544
AlwNI	CAGNNNCTG	1 cut(s)	1873
Aor13HI	TCCGGA	1 cut(s)	1802
Aor51HI	AGCGCT	1 cut(s)	256
AoxI	GGCC	6 cut(s)	159, 334, 528, 1159, 1542, 1869
ApaI	GGGCCC	1 cut(s)	532
ApaLI	GTGCAC	1 cut(s)	323
ApeKI	GCWGC	5 cut(s)	419, 584, 647, 650, 1952
ApoI	RAATTY	3 cut(s)	1215, 1354, 1361
ArsI	GACNNNNNNTTYG	4 cut(s)	36, 68, 764, 796
AsiGI	ACCGGT	1 cut(s)	2088
AspA2I	CCTAGG	1 cut(s)	1220
AspLEI	GCGC	2 cut(s)	196, 257
AspS9I	GGNCC	7 cut(s)	335, 528, 529, 772, 1159, 1870, 2086
AsuC2I	CCSGG	3 cut(s)	56, 287, 338
AsuHPI	GGTGA	4 cut(s)	151, 1409, 1582, 2063
AsuNHI	GCTAGC	2 cut(s)	475, 542
AvaII	GGWCC	2 cut(s)	772, 2086
AvrII	CCTAGG	1 cut(s)	1220
BaeGI	GKGCMC	2 cut(s)	327, 532
BanI	GGYRCC	1 cut(s)	387
BanII	GRGCYC	2 cut(s)	310, 532
BbsI	GAAGAC	1 cut(s)	784
Bbv12I	GWGCWC	3 cut(s)	327, 1314, 1980
BbvI	GCAGC	5 cut(s)	406, 596, 634, 637, 1964
BccI	CCATC	7 cut(s)	218, 353, 896, 1028, 1619, 1802, 1962
BceAI	ACGGC	1 cut(s)	174
BcgI	CGANNNNNNTGC	2 cut(s)	1647, 1681
BciT130I	CCWGG	2 cut(s)	1229, 1874
BcnI	CCSGG	3 cut(s)	56, 287, 338
BcoDI	GTCTC	3 cut(s)	80, 547, 2026
BfaI	CTAG	4 cut(s)	476, 543, 1221, 1787
BfmI	CTRYAG	3 cut(s)	230, 582, 1038
BfoI	RGCGCY	1 cut(s)	258
BglII	AGATCT	1 cut(s)	1011
BisI	GCNGC	6 cut(s)	420, 585, 648, 651, 1452, 1953
BlnI	CCTAGG	1 cut(s)	1220
BlsI	GCNGC	6 cut(s)	421, 586, 649, 652, 1453, 1954
BmcAI	AGTACT	1 cut(s)	127
Bme1390I	CCNGG	5 cut(s)	56, 287, 338, 1229, 1874
Bme18I	GGWCC	2 cut(s)	772, 2086
BmgT120I	GGNCC	7 cut(s)	335, 528, 529, 772, 1159, 1870, 2086
BmiI	GGNNCC	4 cut(s)	309, 389, 530, 1226
BmrFI	CCNGG	5 cut(s)	56, 287, 338, 1229, 1874
BmsI	GCATC	1 cut(s)	1312
BmtI	GCTAGC	2 cut(s)	479, 546
BpiI	GAAGAC	1 cut(s)	784
BpmI	CTGGAG	1 cut(s)	1014
Bpu10I	CCTNAGC	1 cut(s)	383
BpuEI	CTTGAG	2 cut(s)	243, 1028
BpuMI	CCSGG	3 cut(s)	56, 287, 338
BsaAI	YACGTR	1 cut(s)	1373
BsaHI	GRCGYC	1 cut(s)	51
BsaJI	CCNNGG	9 cut(s)	217, 328, 843, 1155, 1220, 1566, 1633, 1781, 1872
BsaWI	WCCGGW	2 cut(s)	1802, 2088
Bsc4I	CCNNNNNNNGG	4 cut(s)	61, 1879, 2032, 2095
Bse118I	RCCGGY	2 cut(s)	111, 2088
Bse1I	ACTGG	4 cut(s)	1061, 1086, 1216, 1743
Bse3DI	GCAATG	1 cut(s)	1479
BseAI	TCCGGA	1 cut(s)	1802
BseBI	CCWGG	2 cut(s)	1229, 1874
BseDI	CCNNGG	9 cut(s)	217, 328, 843, 1155, 1220, 1566, 1633, 1781, 1872
BseGI	GGATG	3 cut(s)	748, 1427, 1973
BseLI	CCNNNNNNNGG	4 cut(s)	61, 1879, 2032, 2095
BseMI	GCAATG	1 cut(s)	1479
BseMII	CTCAG	1 cut(s)	1118
BseNI	ACTGG	4 cut(s)	1061, 1086, 1216, 1743
BseRI	GAGGAG	1 cut(s)	1898
BseSI	GKGCMC	2 cut(s)	327, 532
BseXI	GCAGC	5 cut(s)	406, 596, 634, 637, 1964
BseYI	CCCAGC	1 cut(s)	135
Bsh1236I	CGCG	2 cut(s)	194, 1454
BshFI	GGCC	6 cut(s)	161, 336, 530, 1161, 1544, 1871
BshNI	GGYRCC	1 cut(s)	387
BshTI	ACCGGT	1 cut(s)	2088
BsiHKAI	GWGCWC	3 cut(s)	327, 1314, 1980
BsiSI	CCGG	6 cut(s)	55, 112, 287, 338, 1803, 2089
BslFI	GGGAC	1 cut(s)	62
BslI	CCNNNNNNNGG	4 cut(s)	61, 1879, 2032, 2095
BsmAI	GTCTC	3 cut(s)	80, 547, 2026
BsmFI	GGGAC	1 cut(s)	62
BsmI	GAATGC	1 cut(s)	859
BsnI	GGCC	6 cut(s)	161, 336, 530, 1161, 1544, 1871
Bsp120I	GGGCCC	1 cut(s)	528
Bsp1286I	GDGCHC	5 cut(s)	310, 327, 532, 1314, 1980
Bsp13I	TCCGGA	1 cut(s)	1802
Bsp1407I	TGTACA	2 cut(s)	837, 1027
Bsp143I	GATC	7 cut(s)	204, 291, 440, 721, 1011, 1118, 1549
Bsp19I	CCATGG	2 cut(s)	1155, 1781
BspACI	CCGC	4 cut(s)	1199, 1406, 1452, 2001
BspANI	GGCC	6 cut(s)	161, 336, 530, 1161, 1544, 1871
BspCNI	CTCAG	1 cut(s)	1119
BspEI	TCCGGA	1 cut(s)	1802
BspFNI	CGCG	2 cut(s)	194, 1454
BspHI	TCATGA	1 cut(s)	613
BspLI	GGNNCC	4 cut(s)	309, 389, 530, 1226
BspMAI	CTGCAG	2 cut(s)	234, 586
BspOI	GCTAGC	2 cut(s)	479, 546
BspPI	GGATC	1 cut(s)	1544
BspT107I	GGYRCC	1 cut(s)	387
BsrDI	GCAATG	1 cut(s)	1479
BsrFI	RCCGGY	2 cut(s)	111, 2088
BsrGI	TGTACA	2 cut(s)	837, 1027
BsrI	ACTGG	4 cut(s)	1061, 1086, 1216, 1743
BssAI	RCCGGY	2 cut(s)	111, 2088
BssECI	CCNNGG	9 cut(s)	217, 328, 843, 1155, 1220, 1566, 1633, 1781, 1872
BssMI	GATC	7 cut(s)	204, 291, 440, 721, 1011, 1118, 1549
BssNAI	GTATAC	1 cut(s)	1089
BssNI	GRCGYC	1 cut(s)	51
BssT1I	CCWWGG	8 cut(s)	217, 328, 843, 1155, 1220, 1566, 1633, 1781
Bst1107I	GTATAC	1 cut(s)	1089
Bst2UI	CCWGG	2 cut(s)	1229, 1874
Bst4CI	ACNGT	5 cut(s)	190, 922, 1267, 1467, 1982
Bst6I	CTCTTC	1 cut(s)	1114
BstACI	GRCGYC	1 cut(s)	51
BstAUI	TGTACA	2 cut(s)	837, 1027
BstBAI	YACGTR	1 cut(s)	1373
BstC8I	GCNNGC	7 cut(s)	113, 477, 544, 1067, 1854, 1869, 1957
BstDEI	CTNAG	6 cut(s)	68, 383, 437, 557, 1127, 1991
BstDSI	CCRYGG	2 cut(s)	1155, 1781
BstF5I	GGATG	3 cut(s)	748, 1427, 1973
BstFNI	CGCG	2 cut(s)	194, 1454
BstH2I	RGCGCY	1 cut(s)	258
BstHHI	GCGC	2 cut(s)	196, 257
BstKTI	GATC	7 cut(s)	207, 294, 443, 724, 1014, 1121, 1552
BstMAI	GTCTC	3 cut(s)	80, 547, 2026
BstMBI	GATC	7 cut(s)	204, 291, 440, 721, 1011, 1118, 1549
BstMWI	GCNNNNNNNGC	3 cut(s)	191, 296, 305
BstNI	CCWGG	2 cut(s)	1229, 1874
BstNSI	RCATGY	2 cut(s)	1406, 1496
BstSCI	CCNGG	5 cut(s)	54, 285, 336, 1227, 1872
BstSFI	CTRYAG	3 cut(s)	230, 582, 1038
BstSLI	GKGCMC	2 cut(s)	327, 532
BstSNI	TACGTA	1 cut(s)	1373
BstUI	CGCG	2 cut(s)	194, 1454
BstV1I	GCAGC	5 cut(s)	406, 596, 634, 637, 1964
BstV2I	GAAGAC	1 cut(s)	784
BstX2I	RGATCY	1 cut(s)	1011
BstXI	CCANNNNNNTGG	4 cut(s)	224, 782, 895, 1640
BstYI	RGATCY	1 cut(s)	1011
BstZ17I	GTATAC	1 cut(s)	1089
BsuRI	GGCC	6 cut(s)	161, 336, 530, 1161, 1544, 1871
BtgI	CCRYGG	2 cut(s)	1155, 1781
BtsCI	GGATG	3 cut(s)	748, 1427, 1973
BtsI	GCAGTG	3 cut(s)	348, 470, 553
BtsIMutI	CAGTG	4 cut(s)	348, 470, 553, 1079
Cac8I	GCNNGC	7 cut(s)	113, 477, 544, 1067, 1854, 1869, 1957
CaiI	CAGNNNCTG	1 cut(s)	1873
CciI	TCATGA	1 cut(s)	613
CfoI	GCGC	2 cut(s)	196, 257
Cfr10I	RCCGGY	2 cut(s)	111, 2088
Cfr13I	GGNCC	7 cut(s)	335, 528, 529, 772, 1159, 1870, 2086
CspAI	ACCGGT	1 cut(s)	2088
DdeI	CTNAG	6 cut(s)	68, 383, 437, 557, 1127, 1991
DpnI	GATC	7 cut(s)	206, 293, 442, 723, 1013, 1120, 1551
DpnII	GATC	7 cut(s)	204, 291, 440, 721, 1011, 1118, 1549
DraI	TTTAAA	2 cut(s)	601, 2103
DraIII	CACNNNGTG	1 cut(s)	23
DrdI	GACNNNNNNGTC	1 cut(s)	1436
DseDI	GACNNNNNNGTC	1 cut(s)	1436
Eam1104I	CTCTTC	1 cut(s)	1114
EarI	CTCTTC	1 cut(s)	1114
EciI	GGCGGA	1 cut(s)	2016
Eco105I	TACGTA	1 cut(s)	1373
Eco130I	CCWWGG	8 cut(s)	217, 328, 843, 1155, 1220, 1566, 1633, 1781
Eco147I	AGGCCT	1 cut(s)	1544
Eco24I	GRGCYC	2 cut(s)	310, 532
Eco47I	GGWCC	2 cut(s)	772, 2086
Eco47III	AGCGCT	1 cut(s)	256
EcoO109I	RGGNCCY	2 cut(s)	529, 1870
EcoRI	GAATTC	2 cut(s)	1215, 1354
EcoRII	CCWGG	2 cut(s)	1227, 1872
EcoT14I	CCWWGG	8 cut(s)	217, 328, 843, 1155, 1220, 1566, 1633, 1781
EcoT22I	ATGCAT	1 cut(s)	691
EcoT38I	GRGCYC	2 cut(s)	310, 532
ErhI	CCWWGG	8 cut(s)	217, 328, 843, 1155, 1220, 1566, 1633, 1781
FaqI	GGGAC	1 cut(s)	62
FblI	GTMKAC	1 cut(s)	1088
Fnu4HI	GCNGC	6 cut(s)	420, 585, 648, 651, 1452, 1953
FokI	GGATG	3 cut(s)	755, 1434, 1980
FriOI	GRGCYC	2 cut(s)	310, 532
Fsp4HI	GCNGC	6 cut(s)	420, 585, 648, 651, 1452, 1953
FspBI	CTAG	4 cut(s)	476, 543, 1221, 1787
GlaI	GCGC	2 cut(s)	195, 256
GluI	GCNGC	6 cut(s)	420, 585, 648, 651, 1452, 1953
GsaI	CCCAGC	1 cut(s)	139
GsuI	CTGGAG	1 cut(s)	1014
HaeII	RGCGCY	1 cut(s)	258
HaeIII	GGCC	6 cut(s)	161, 336, 530, 1161, 1544, 1871
HapII	CCGG	6 cut(s)	55, 112, 287, 338, 1803, 2089
HhaI	GCGC	2 cut(s)	196, 257
Hin1I	GRCGYC	1 cut(s)	51
Hin6I	GCGC	2 cut(s)	194, 255
HinP1I	GCGC	2 cut(s)	194, 255
HincII	GTYRAC	2 cut(s)	1528, 1985
HindII	GTYRAC	2 cut(s)	1528, 1985
HpaII	CCGG	6 cut(s)	55, 112, 287, 338, 1803, 2089
HphI	GGTGA	4 cut(s)	151, 1409, 1582, 2063
Hpy188I	TCNGA	9 cut(s)	500, 591, 757, 884, 1128, 1150, 1549, 1588, 1771
Hpy188III	TCNNGA	5 cut(s)	614, 770, 1319, 1384, 1803
HpyAV	CCTTC	5 cut(s)	674, 1325, 1692, 1835, 2036
HpyCH4III	ACNGT	5 cut(s)	190, 922, 1267, 1467, 1982
HpyCH4IV	ACGT	2 cut(s)	51, 1372
HpyF10VI	GCNNNNNNNGC	3 cut(s)	191, 296, 305
HpyF3I	CTNAG	6 cut(s)	68, 383, 437, 557, 1127, 1991
HpySE526I	ACGT	2 cut(s)	51, 1372
Hsp92I	GRCGYC	1 cut(s)	51
HspAI	GCGC	2 cut(s)	194, 255
Kpn2I	TCCGGA	1 cut(s)	1802
KroI	GCCGGC	1 cut(s)	111
KroNI	GCCGGC	1 cut(s)	113
Kzo9I	GATC	7 cut(s)	204, 291, 440, 721, 1011, 1118, 1549
LmnI	GCTCC	4 cut(s)	313, 461, 995, 1975
Lsp1109I	GCAGC	5 cut(s)	406, 596, 634, 637, 1964
LweI	GCATC	1 cut(s)	1312
MaeI	CTAG	4 cut(s)	476, 543, 1221, 1787
MaeII	ACGT	2 cut(s)	51, 1372
MaeIII	GTNAC	1 cut(s)	2091
MalI	GATC	7 cut(s)	206, 293, 442, 723, 1013, 1120, 1551
MboI	GATC	7 cut(s)	204, 291, 440, 721, 1011, 1118, 1549
MboII	GAAGA	5 cut(s)	308, 784, 958, 1101, 1913
MfeI	CAATTG	1 cut(s)	999
MflI	RGATCY	1 cut(s)	1011
MhlI	GDGCHC	5 cut(s)	310, 327, 532, 1314, 1980
MlyI	GAGTC	3 cut(s)	586, 1679, 1808
MmeI	TCCRAC	3 cut(s)	342, 478, 1711
Mph1103I	ATGCAT	1 cut(s)	691
MroI	TCCGGA	1 cut(s)	1802
MroNI	GCCGGC	1 cut(s)	111
MseI	TTAA	3 cut(s)	600, 1061, 2102
MslI	CAYNNNNRTG	1 cut(s)	330
MspI	CCGG	6 cut(s)	55, 112, 287, 338, 1803, 2089
MspR9I	CCNGG	5 cut(s)	56, 287, 338, 1229, 1874
MunI	CAATTG	1 cut(s)	999
Mva1269I	GAATGC	1 cut(s)	859
MvaI	CCWGG	2 cut(s)	1229, 1874
MvnI	CGCG	2 cut(s)	194, 1454
MwoI	GCNNNNNNNGC	3 cut(s)	191, 296, 305
NaeI	GCCGGC	1 cut(s)	113
NciI	CCSGG	3 cut(s)	56, 287, 338
NcoI	CCATGG	2 cut(s)	1155, 1781
NdeII	GATC	7 cut(s)	204, 291, 440, 721, 1011, 1118, 1549
NgoMIV	GCCGGC	1 cut(s)	111
NheI	GCTAGC	2 cut(s)	475, 542
NlaIV	GGNNCC	4 cut(s)	309, 389, 530, 1226
NsiI	ATGCAT	1 cut(s)	691
NspI	RCATGY	2 cut(s)	1406, 1496
OliI	CACNNNNGTG	1 cut(s)	330
PagI	TCATGA	1 cut(s)	613
PceI	AGGCCT	1 cut(s)	1544
PcsI	WCGNNNNNNNCGW	1 cut(s)	48
PctI	GAATGC	1 cut(s)	859
PdiI	GCCGGC	1 cut(s)	113
PfeI	GAWTC	7 cut(s)	144, 501, 563, 610, 766, 1151, 1777
PinAI	ACCGGT	1 cut(s)	2088
PkrI	GCNGC	6 cut(s)	421, 586, 649, 652, 1453, 1954
PleI	GAGTC	3 cut(s)	586, 1678, 1807
PpsI	GAGTC	3 cut(s)	586, 1678, 1807
Ppu21I	YACGTR	1 cut(s)	1373
Psp6I	CCWGG	2 cut(s)	1227, 1872
PspFI	CCCAGC	1 cut(s)	135
PspGI	CCWGG	2 cut(s)	1227, 1872
PspN4I	GGNNCC	4 cut(s)	309, 389, 530, 1226
PspOMI	GGGCCC	1 cut(s)	528
PspPI	GGNCC	7 cut(s)	335, 528, 529, 772, 1159, 1870, 2086
PstI	CTGCAG	2 cut(s)	234, 586
PstNI	CAGNNNCTG	1 cut(s)	1873
PsuI	RGATCY	1 cut(s)	1011
RseI	CAYNNNNRTG	1 cut(s)	330
SaqAI	TTAA	3 cut(s)	600, 1061, 2102
SatI	GCNGC	6 cut(s)	420, 585, 648, 651, 1452, 1953
Sau3AI	GATC	7 cut(s)	204, 291, 440, 721, 1011, 1118, 1549
Sau96I	GGNCC	7 cut(s)	335, 528, 529, 772, 1159, 1870, 2086
ScaI	AGTACT	1 cut(s)	127
SchI	GAGTC	3 cut(s)	586, 1679, 1808
ScrFI	CCNGG	5 cut(s)	56, 287, 338, 1229, 1874
SduI	GDGCHC	5 cut(s)	310, 327, 532, 1314, 1980
SfaNI	GCATC	1 cut(s)	1312
SfcI	CTRYAG	3 cut(s)	230, 582, 1038
SinI	GGWCC	2 cut(s)	772, 2086
SmiMI	CAYNNNNRTG	1 cut(s)	330
SmlI	CTYRAG	2 cut(s)	258, 1007
SmoI	CTYRAG	2 cut(s)	258, 1007
SnaBI	TACGTA	1 cut(s)	1373
SseBI	AGGCCT	1 cut(s)	1544
SsiI	CCGC	4 cut(s)	1199, 1406, 1452, 2001
SspMI	CTAG	4 cut(s)	476, 543, 1221, 1787
StuI	AGGCCT	1 cut(s)	1544
StyD4I	CCNGG	5 cut(s)	54, 285, 336, 1227, 1872
StyI	CCWWGG	8 cut(s)	217, 328, 843, 1155, 1220, 1566, 1633, 1781
TaaI	ACNGT	5 cut(s)	190, 922, 1267, 1467, 1982
TaiI	ACGT	2 cut(s)	54, 1375
TaqI	TCGA	3 cut(s)	1117, 1657, 1924
TatI	WGTACW	6 cut(s)	125, 837, 1027, 1053, 1208, 1424
TauI	GCSGC	1 cut(s)	1454
TfiI	GAWTC	7 cut(s)	144, 501, 563, 610, 766, 1151, 1777
Tru1I	TTAA	3 cut(s)	600, 1061, 2102
Tru9I	TTAA	3 cut(s)	600, 1061, 2102
TscAI	CASTG	4 cut(s)	355, 477, 553, 1086
TseI	GCWGC	5 cut(s)	419, 584, 647, 650, 1952
TspDTI	ATGAA	8 cut(s)	768, 922, 1101, 1307, 1473, 1612, 1778, 1914
TspGWI	ACGGA	3 cut(s)	300, 1895, 1988
TspRI	CASTG	4 cut(s)	355, 477, 553, 1086
VneI	GTGCAC	1 cut(s)	323
VpaK11BI	GGWCC	2 cut(s)	772, 2086
XapI	RAATTY	3 cut(s)	1215, 1354, 1361
XceI	RCATGY	2 cut(s)	1406, 1496
XmaJI	CCTAGG	1 cut(s)	1220
XmiI	GTMKAC	1 cut(s)	1088
XspI	CTAG	4 cut(s)	476, 543, 1221, 1787
ZraI	GACGTC	1 cut(s)	52
ZrmI	AGTACT	1 cut(s)	127
Zsp2I	ATGCAT	1 cut(s)	691

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.