Rh1BG108300

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0004257
Physical Location & Seq
Forward (+)
18718603 .. 18721477
2875 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG108300.1

Sequence Viewer

Length: 597 bp
ATGCGCCGAAGGCAAGAAGCGACGGGAATCCGGATCCGACGACGAAACTGGGTCGAGGTCCGAGAAGAAGAGAATCAGAAGAAAGAGAGGAAAATGGAGAGAGAAGAAGACGACTCAGTGAGCGAGGTTCTCAGAGATCGATTCAGACTCTCCACCATCTATATCGCCGATGCCGAAGTTTTCAATTCTAGTTCTCCAAGCAGCGAAGAGAAGCGGCATGGAGATATCGGAACCCGTGGTGGCTTGCATTTCCGACTTGGCCTTCAAATTTACTGGTCTCTTTGCTTTGGAGACATGGAGGCCACGATGCCCATTTGCAAGACTGATACTCCAACTCTGAGATGGGTGAAGCAGTCAAGGAAGCTTATTTACAACAAATCCAAACAGTACACCAAGGAGTACGCGGAGAAGGACAATGAGTTGGTCCGGTTGAAGCGTGAAGCAAGGCTGAAAGGAAGATTCTATGTCAAGCCAGAGTCTAAGCTTTTGTTTATCATTCGTATCCATGGTATCAATGCCATTGATCCAAAGACAAAGAAGATCTTGCAACTTTTAAGTTGGAAATGCATCTACGAGTATGAAAATAAGAATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L30 Ribosomal_L30_N

Protein Analysis

198

Amino Acids

23.73

Weight (kDa)

9.7

Isoelectric Point (pI)

59.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L30_N PF08079 117 - 157 5.1e-11 Ribosomal L30 N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 404
AccIII TCCGGA 1 cut(s) 30
AciI CCGC 2 cut(s) 214, 404
AclWI GGATC 3 cut(s) 28, 41, 518
AcsI RAATTY 2 cut(s) 267, 589
AfaI GTAC 2 cut(s) 389, 401
AgsI TTSAA 3 cut(s) 184, 266, 433
AluBI AGCT 2 cut(s) 364, 484
AluI AGCT 2 cut(s) 364, 484
Alw26I GTCTC 2 cut(s) 282, 285
AlwI GGATC 3 cut(s) 28, 41, 518
Aor13HI TCCGGA 1 cut(s) 30
AoxI GGCC 2 cut(s) 259, 300
ApeKI GCWGC 1 cut(s) 201
ApoI RAATTY 2 cut(s) 267, 589
AspLEI GCGC 1 cut(s) 6
AspS9I GGNCC 2 cut(s) 58, 424
AsuHPI GGTGA 1 cut(s) 358
AvaII GGWCC 2 cut(s) 58, 424
BamHI GGATCC 1 cut(s) 33
BarI GAAGNNNNNNTAC 2 cut(s) 353, 385
BbsI GAAGAC 1 cut(s) 114
BbvI GCAGC 1 cut(s) 213
BccI CCATC 2 cut(s) 164, 336
BciVI GTATCC 1 cut(s) 512
BcoDI GTCTC 2 cut(s) 282, 285
BfaI CTAG 1 cut(s) 189
BfuI GTATCC 1 cut(s) 512
BglII AGATCT 1 cut(s) 540
BisI GCNGC 2 cut(s) 202, 215
BlsI GCNGC 2 cut(s) 203, 216
Bme18I GGWCC 2 cut(s) 58, 424
BmgT120I GGNCC 2 cut(s) 58, 424
BmiI GGNNCC 2 cut(s) 35, 232
BmrI ACTGGG 1 cut(s) 58
BmsI GCATC 3 cut(s) 160, 297, 576
BmuI ACTGGG 1 cut(s) 58
BpiI GAAGAC 1 cut(s) 114
Bsa29I ATCGAT 1 cut(s) 139
BsaI GGTCTC 1 cut(s) 282
BsaJI CCNNGG 3 cut(s) 235, 393, 505
BsaWI WCCGGW 2 cut(s) 30, 426
BsaXI ACNNNNNCTCC 2 cut(s) 313, 343
Bse1I ACTGG 2 cut(s) 53, 278
BseAI TCCGGA 1 cut(s) 30
BseCI ATCGAT 1 cut(s) 139
BseDI CCNNGG 3 cut(s) 235, 393, 505
BseMII CTCAG 3 cut(s) 129, 145, 329
BseNI ACTGG 2 cut(s) 53, 278
BseXI GCAGC 1 cut(s) 213
Bsh1236I CGCG 1 cut(s) 404
BshFI GGCC 2 cut(s) 261, 302
BshVI ATCGAT 1 cut(s) 139
BsiSI CCGG 2 cut(s) 31, 427
BsmAI GTCTC 2 cut(s) 282, 285
BsnI GGCC 2 cut(s) 261, 302
Bso31I GGTCTC 1 cut(s) 282
Bsp13I TCCGGA 1 cut(s) 30
Bsp143I GATC 4 cut(s) 33, 136, 523, 540
Bsp19I CCATGG 1 cut(s) 505
BspACI CCGC 2 cut(s) 214, 404
BspANI GGCC 2 cut(s) 261, 302
BspCNI CTCAG 3 cut(s) 128, 144, 330
BspDI ATCGAT 1 cut(s) 139
BspEI TCCGGA 1 cut(s) 30
BspFNI CGCG 1 cut(s) 404
BspLI GGNNCC 2 cut(s) 35, 232
BspPI GGATC 3 cut(s) 28, 41, 518
BspTNI GGTCTC 1 cut(s) 282
BsrI ACTGG 2 cut(s) 53, 278
BssECI CCNNGG 3 cut(s) 235, 393, 505
BssMI GATC 4 cut(s) 33, 136, 523, 540
BssT1I CCWWGG 2 cut(s) 393, 505
Bst4CI ACNGT 1 cut(s) 387
Bst6I CTCTTC 2 cut(s) 63, 201
BstC8I GCNNGC 1 cut(s) 245
BstDEI CTNAG 4 cut(s) 115, 131, 338, 480
BstDSI CCRYGG 2 cut(s) 235, 505
BstFNI CGCG 1 cut(s) 404
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 4 cut(s) 36, 139, 526, 543
BstMAI GTCTC 2 cut(s) 282, 285
BstMBI GATC 4 cut(s) 33, 136, 523, 540
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstUI CGCG 1 cut(s) 404
BstV1I GCAGC 1 cut(s) 213
BstV2I GAAGAC 1 cut(s) 114
BstX2I RGATCY 2 cut(s) 33, 540
BstYI RGATCY 2 cut(s) 33, 540
Bsu15I ATCGAT 1 cut(s) 139
BsuI GTATCC 1 cut(s) 512
BsuRI GGCC 2 cut(s) 261, 302
BsuTUI ATCGAT 1 cut(s) 139
BtgI CCRYGG 2 cut(s) 235, 505
BtsIMutI CAGTG 1 cut(s) 123
Cac8I GCNNGC 1 cut(s) 245
CfoI GCGC 1 cut(s) 6
Cfr13I GGNCC 2 cut(s) 58, 424
ClaI ATCGAT 1 cut(s) 139
Csp6I GTAC 2 cut(s) 388, 400
CviAII CATG 3 cut(s) 218, 295, 506
CviJI RGCY 7 cut(s) 243, 261, 302, 364, 448, 472, 484
CviKI_1 RGCY 7 cut(s) 243, 261, 302, 364, 448, 472, 484
CviQI GTAC 2 cut(s) 388, 400
DdeI CTNAG 4 cut(s) 115, 131, 338, 480
DpnI GATC 4 cut(s) 35, 138, 525, 542
DpnII GATC 4 cut(s) 33, 136, 523, 540
Eam1104I CTCTTC 2 cut(s) 63, 201
EarI CTCTTC 2 cut(s) 63, 201
Eco130I CCWWGG 2 cut(s) 393, 505
Eco31I GGTCTC 1 cut(s) 282
Eco32I GATATC 1 cut(s) 226
Eco47I GGWCC 2 cut(s) 58, 424
EcoRV GATATC 1 cut(s) 226
EcoT14I CCWWGG 2 cut(s) 393, 505
EcoT22I ATGCAT 1 cut(s) 569
ErhI CCWWGG 2 cut(s) 393, 505
FaeI CATG 3 cut(s) 221, 298, 509
FaiI YATR 6 cut(s) 162, 219, 296, 465, 507, 579
FalI AAGNNNNNCTT 2 cut(s) 527, 559
FatI CATG 3 cut(s) 217, 294, 505
Fnu4HI GCNGC 2 cut(s) 202, 215
Fsp4HI GCNGC 2 cut(s) 202, 215
FspBI CTAG 1 cut(s) 189
GlaI GCGC 1 cut(s) 5
GluI GCNGC 2 cut(s) 202, 215
HaeIII GGCC 2 cut(s) 261, 302
HapII CCGG 2 cut(s) 31, 427
HhaI GCGC 1 cut(s) 6
Hin1II CATG 3 cut(s) 221, 298, 509
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HindIII AAGCTT 2 cut(s) 362, 482
HinfI GANTC 7 cut(s) 27, 73, 113, 141, 147, 459, 476
HpaII CCGG 2 cut(s) 31, 427
HphI GGTGA 1 cut(s) 358
Hpy166II GTNNAC 1 cut(s) 390
Hpy188I TCNGA 8 cut(s) 38, 62, 78, 134, 146, 230, 254, 339
Hpy188III TCNNGA 1 cut(s) 31
Hpy8I GTNNAC 1 cut(s) 390
Hpy99I CGWCG 3 cut(s) 25, 42, 45
HpyAV CCTTC 3 cut(s) 3, 272, 403
HpyCH4III ACNGT 1 cut(s) 387
HpyCH4V TGCA 4 cut(s) 247, 318, 547, 567
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 4 cut(s) 115, 131, 338, 480
Hsp92II CATG 3 cut(s) 221, 298, 509
HspAI GCGC 1 cut(s) 4
Kpn2I TCCGGA 1 cut(s) 30
Kzo9I GATC 4 cut(s) 33, 136, 523, 540
LpnPI CCDG 5 cut(s) 34, 44, 259, 440, 486
Lsp1109I GCAGC 1 cut(s) 213
LweI GCATC 3 cut(s) 160, 297, 576
MaeI CTAG 1 cut(s) 189
MalI GATC 4 cut(s) 35, 138, 525, 542
MboI GATC 4 cut(s) 33, 136, 523, 540
MboII GAAGA 8 cut(s) 77, 80, 91, 116, 119, 218, 468, 550
MflI RGATCY 2 cut(s) 33, 540
MluCI AATT 3 cut(s) 184, 267, 589
MlyI GAGTC 3 cut(s) 107, 141, 485
MmeI TCCRAC 4 cut(s) 61, 277, 356, 539
MnlI CCTC 4 cut(s) 49, 81, 118, 292
Mph1103I ATGCAT 1 cut(s) 569
MroI TCCGGA 1 cut(s) 30
MseI TTAA 1 cut(s) 554
MspI CCGG 2 cut(s) 31, 427
MvnI CGCG 1 cut(s) 404
MwoI GCNNNNNNNGC 1 cut(s) 10
NcoI CCATGG 1 cut(s) 505
NdeII GATC 4 cut(s) 33, 136, 523, 540
NlaIII CATG 3 cut(s) 221, 298, 509
NlaIV GGNNCC 2 cut(s) 35, 232
NsiI ATGCAT 1 cut(s) 569
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PfeI GAWTC 4 cut(s) 27, 73, 141, 459
PkrI GCNGC 2 cut(s) 203, 216
PleI GAGTC 3 cut(s) 107, 141, 484
PpsI GAGTC 3 cut(s) 107, 141, 484
PspN4I GGNNCC 2 cut(s) 35, 232
PspPI GGNCC 2 cut(s) 58, 424
PsuI RGATCY 2 cut(s) 33, 540
RsaI GTAC 2 cut(s) 389, 401
RsaNI GTAC 2 cut(s) 388, 400
SaqAI TTAA 1 cut(s) 554
SatI GCNGC 2 cut(s) 202, 215
Sau3AI GATC 4 cut(s) 33, 136, 523, 540
Sau96I GGNCC 2 cut(s) 58, 424
SchI GAGTC 3 cut(s) 107, 141, 485
SetI ASST 4 cut(s) 60, 129, 366, 486
SfaNI GCATC 3 cut(s) 160, 297, 576
SinI GGWCC 2 cut(s) 58, 424
Sse9I AATT 3 cut(s) 184, 267, 589
SsiI CCGC 2 cut(s) 214, 404
SspMI CTAG 1 cut(s) 189
StyI CCWWGG 2 cut(s) 393, 505
TaaI ACNGT 1 cut(s) 387
TaqI TCGA 2 cut(s) 54, 139
TasI AATT 3 cut(s) 184, 267, 589
TatI WGTACW 1 cut(s) 387
TauI GCSGC 1 cut(s) 217
TfiI GAWTC 4 cut(s) 27, 73, 141, 459
Tru1I TTAA 1 cut(s) 554
Tru9I TTAA 1 cut(s) 554
TscAI CASTG 1 cut(s) 123
TseI GCWGC 1 cut(s) 201
TspDTI ATGAA 1 cut(s) 594
TspRI CASTG 1 cut(s) 123
VpaK11BI GGWCC 2 cut(s) 58, 424
XapI RAATTY 2 cut(s) 267, 589
XcmI CCANNNNNNNNNTGG 1 cut(s) 339
XspI CTAG 1 cut(s) 189
Zsp2I ATGCAT 1 cut(s) 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.