Rw7G037910

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0004257
Physical Location & Seq
Forward (+)
57540861 .. 57543948
3088 bp
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UTR
Exon/CDS
Intron
Rw7G037910.1

Sequence Viewer

Length: 381 bp
ATGGGTGAAGCAGTCAAGGTAGTGGTTCCGGAGTCTGTTCTAAAGAAGAGGAAGAGAGAGGAGGAATGGGCCTTGGCAAAGAAGCAGGGGCTTGAAGCCACCAAGAAAAAGAATGCTGAGAATAGGAAGCTTATTTACAAAAAGTACACCAAGGAGTACGCAGAGAAGGACAATGAGTTGGTCCGGTTGAAGCGTGAAACAAGGCTGAAGGGAGGATTCTATGTCAAGCCAGAGTCTAAGCTTTCATTTATCATTCGTGTCCATGGTATCAATGCCATTGATCCAAAGATAAAGAAGATCTTGCAACTTTTATACCCCAATCTGAAGAATGTGAAGGAATTGATTTACGAGACAATTTATTGTAATAACTTGGTGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L30 Ribosomal_L30_N

Protein Analysis

126

Amino Acids

14.85

Weight (kDa)

9.89

Isoelectric Point (pI)

28.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L30_N PF08079 9 - 76 2.8e-18 Ribosomal L30 N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 28
AciI CCGC 1 cut(s) 376
AclWI GGATC 1 cut(s) 275
AcuI CTGAAG 2 cut(s) 227, 344
AfaI GTAC 2 cut(s) 146, 158
AgsI TTSAA 2 cut(s) 95, 190
AluBI AGCT 2 cut(s) 130, 241
AluI AGCT 2 cut(s) 130, 241
Alw26I GTCTC 1 cut(s) 344
AlwI GGATC 1 cut(s) 275
Aor13HI TCCGGA 1 cut(s) 28
AoxI GGCC 1 cut(s) 69
AspS9I GGNCC 2 cut(s) 69, 181
AsuHPI GGTGA 1 cut(s) 17
AvaII GGWCC 1 cut(s) 181
BarI GAAGNNNNNNTAC 2 cut(s) 119, 151
BcoDI GTCTC 1 cut(s) 344
BglII AGATCT 1 cut(s) 297
Bme18I GGWCC 1 cut(s) 181
BmgT120I GGNCC 2 cut(s) 69, 181
BmiI GGNNCC 1 cut(s) 27
BsaJI CCNNGG 3 cut(s) 72, 150, 262
BsaWI WCCGGW 2 cut(s) 28, 183
BseAI TCCGGA 1 cut(s) 28
BseDI CCNNGG 3 cut(s) 72, 150, 262
BseMII CTCAG 1 cut(s) 108
BseRI GAGGAG 1 cut(s) 74
BshFI GGCC 1 cut(s) 71
BsiSI CCGG 2 cut(s) 29, 184
BsmAI GTCTC 1 cut(s) 344
BsmI GAATGC 1 cut(s) 118
BsnI GGCC 1 cut(s) 71
Bsp13I TCCGGA 1 cut(s) 28
Bsp143I GATC 2 cut(s) 280, 297
Bsp19I CCATGG 1 cut(s) 262
BspACI CCGC 1 cut(s) 376
BspANI GGCC 1 cut(s) 71
BspCNI CTCAG 1 cut(s) 109
BspEI TCCGGA 1 cut(s) 28
BspLI GGNNCC 1 cut(s) 27
BspPI GGATC 1 cut(s) 275
BssECI CCNNGG 3 cut(s) 72, 150, 262
BssMI GATC 2 cut(s) 280, 297
BssT1I CCWWGG 3 cut(s) 72, 150, 262
Bst6I CTCTTC 2 cut(s) 41, 47
BstDEI CTNAG 2 cut(s) 117, 237
BstDSI CCRYGG 1 cut(s) 262
BstKTI GATC 2 cut(s) 283, 300
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 2 cut(s) 280, 297
BstX2I RGATCY 1 cut(s) 297
BstYI RGATCY 1 cut(s) 297
BsuRI GGCC 1 cut(s) 71
BtgI CCRYGG 1 cut(s) 262
Cfr13I GGNCC 2 cut(s) 69, 181
Csp6I GTAC 2 cut(s) 145, 157
CviAII CATG 1 cut(s) 263
CviJI RGCY 7 cut(s) 71, 91, 98, 130, 205, 229, 241
CviKI_1 RGCY 7 cut(s) 71, 91, 98, 130, 205, 229, 241
CviQI GTAC 2 cut(s) 145, 157
DdeI CTNAG 2 cut(s) 117, 237
DpnI GATC 2 cut(s) 282, 299
DpnII GATC 2 cut(s) 280, 297
Eam1104I CTCTTC 2 cut(s) 41, 47
EarI CTCTTC 2 cut(s) 41, 47
Eco130I CCWWGG 3 cut(s) 72, 150, 262
Eco47I GGWCC 1 cut(s) 181
Eco57I CTGAAG 2 cut(s) 227, 344
EcoT14I CCWWGG 3 cut(s) 72, 150, 262
ErhI CCWWGG 3 cut(s) 72, 150, 262
FaeI CATG 1 cut(s) 266
FaiI YATR 3 cut(s) 222, 264, 313
FalI AAGNNNNNCTT 2 cut(s) 284, 316
FatI CATG 1 cut(s) 262
HaeIII GGCC 1 cut(s) 71
HapII CCGG 2 cut(s) 29, 184
Hin1II CATG 1 cut(s) 266
HindIII AAGCTT 2 cut(s) 128, 239
HinfI GANTC 3 cut(s) 32, 216, 233
HpaII CCGG 2 cut(s) 29, 184
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 1 cut(s) 147
Hpy188I TCNGA 1 cut(s) 324
Hpy188III TCNNGA 1 cut(s) 29
Hpy8I GTNNAC 1 cut(s) 147
HpyAV CCTTC 3 cut(s) 160, 202, 328
HpyCH4V TGCA 1 cut(s) 304
HpyF3I CTNAG 2 cut(s) 117, 237
Hsp92II CATG 1 cut(s) 266
Kpn2I TCCGGA 1 cut(s) 28
Kzo9I GATC 2 cut(s) 280, 297
LpnPI CCDG 4 cut(s) 42, 71, 197, 243
MalI GATC 2 cut(s) 282, 299
MboI GATC 2 cut(s) 280, 297
MboII GAAGA 4 cut(s) 58, 64, 307, 337
MflI RGATCY 1 cut(s) 297
MluCI AATT 2 cut(s) 338, 354
MlyI GAGTC 2 cut(s) 41, 242
MnlI CCTC 4 cut(s) 42, 52, 55, 206
MroI TCCGGA 1 cut(s) 28
MspI CCGG 2 cut(s) 29, 184
Mva1269I GAATGC 1 cut(s) 118
NcoI CCATGG 1 cut(s) 262
NdeII GATC 2 cut(s) 280, 297
NlaIII CATG 1 cut(s) 266
NlaIV GGNNCC 1 cut(s) 27
PctI GAATGC 1 cut(s) 118
PfeI GAWTC 1 cut(s) 216
PleI GAGTC 2 cut(s) 40, 241
PpsI GAGTC 2 cut(s) 40, 241
PspN4I GGNNCC 1 cut(s) 27
PspPI GGNCC 2 cut(s) 69, 181
PsuI RGATCY 1 cut(s) 297
RsaI GTAC 2 cut(s) 146, 158
RsaNI GTAC 2 cut(s) 145, 157
Sau3AI GATC 2 cut(s) 280, 297
Sau96I GGNCC 2 cut(s) 69, 181
SchI GAGTC 2 cut(s) 41, 242
SetI ASST 3 cut(s) 21, 132, 243
SinI GGWCC 1 cut(s) 181
Sse9I AATT 2 cut(s) 338, 354
SsiI CCGC 1 cut(s) 376
StyI CCWWGG 3 cut(s) 72, 150, 262
TasI AATT 2 cut(s) 338, 354
TatI WGTACW 1 cut(s) 144
TfiI GAWTC 1 cut(s) 216
TspDTI ATGAA 1 cut(s) 234
VpaK11BI GGWCC 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.