Rh5DG564800

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004257
Physical Location & Seq
Reverse (-)
87054928 .. 87058979
4052 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG564800.1

Sequence Viewer

Length: 324 bp
ATGGGTGAAGCAGTCAAGGTAGTGCTTTCGGAGTCTGTTCTAAAGAAGAGGAAGAGAGATGAGGAATGGGCCTTGGCAAAGACGCAGGGGCTTGAAGCCGCCAAGAAAAAGAATGCTGAGAATAGGAAGCTTATTTACAACAAATCCAAACAGTACACCAAGGAGTACGCGGAGAAGGACAATGAGTTGGTCCGGTTGAAGCGTGAAGCAAGGCTAAAAGGAGGATTCTATGTCAAGCCAGAGTCTAAGCTTTCGTTTATCATTCGTATCCATGGTATCAATGCCATTGATCCAAAGACAAAGAAGATCTTGCAACTTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L30 Ribosomal_L30_N

Protein Analysis

107

Amino Acids

12.4

Weight (kDa)

10.0

Isoelectric Point (pI)

30.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L30_N PF08079 10 - 79 3e-21 Ribosomal L30 N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 170
AciI CCGC 2 cut(s) 99, 170
AclWI GGATC 1 cut(s) 284
AfaI GTAC 2 cut(s) 155, 167
AgsI TTSAA 2 cut(s) 95, 199
AluBI AGCT 2 cut(s) 130, 250
AluI AGCT 2 cut(s) 130, 250
AlwI GGATC 1 cut(s) 284
AoxI GGCC 1 cut(s) 69
AspS9I GGNCC 2 cut(s) 69, 190
AsuHPI GGTGA 1 cut(s) 17
AvaII GGWCC 1 cut(s) 190
BarI GAAGNNNNNNTAC 2 cut(s) 119, 151
BciVI GTATCC 1 cut(s) 278
BfuI GTATCC 1 cut(s) 278
BglII AGATCT 1 cut(s) 306
BisI GCNGC 1 cut(s) 99
BlsI GCNGC 1 cut(s) 100
Bme18I GGWCC 1 cut(s) 190
BmgT120I GGNCC 2 cut(s) 69, 190
BsaJI CCNNGG 3 cut(s) 72, 159, 271
BsaWI WCCGGW 1 cut(s) 192
BseDI CCNNGG 3 cut(s) 72, 159, 271
BseMII CTCAG 1 cut(s) 108
Bsh1236I CGCG 1 cut(s) 170
BshFI GGCC 1 cut(s) 71
BsiSI CCGG 1 cut(s) 193
BsmI GAATGC 1 cut(s) 118
BsnI GGCC 1 cut(s) 71
Bsp143I GATC 2 cut(s) 289, 306
Bsp19I CCATGG 1 cut(s) 271
BspACI CCGC 2 cut(s) 99, 170
BspANI GGCC 1 cut(s) 71
BspCNI CTCAG 1 cut(s) 109
BspFNI CGCG 1 cut(s) 170
BspPI GGATC 1 cut(s) 284
BssECI CCNNGG 3 cut(s) 72, 159, 271
BssMI GATC 2 cut(s) 289, 306
BssT1I CCWWGG 3 cut(s) 72, 159, 271
Bst4CI ACNGT 1 cut(s) 153
Bst6I CTCTTC 2 cut(s) 41, 47
BstDEI CTNAG 2 cut(s) 117, 246
BstDSI CCRYGG 1 cut(s) 271
BstFNI CGCG 1 cut(s) 170
BstKTI GATC 2 cut(s) 292, 309
BstMBI GATC 2 cut(s) 289, 306
BstUI CGCG 1 cut(s) 170
BstX2I RGATCY 1 cut(s) 306
BstYI RGATCY 1 cut(s) 306
BsuI GTATCC 1 cut(s) 278
BsuRI GGCC 1 cut(s) 71
BtgI CCRYGG 1 cut(s) 271
Cfr13I GGNCC 2 cut(s) 69, 190
CseI GACGC 1 cut(s) 91
Csp6I GTAC 2 cut(s) 154, 166
CviAII CATG 1 cut(s) 272
CviJI RGCY 7 cut(s) 71, 91, 98, 130, 214, 238, 250
CviKI_1 RGCY 7 cut(s) 71, 91, 98, 130, 214, 238, 250
CviQI GTAC 2 cut(s) 154, 166
DdeI CTNAG 2 cut(s) 117, 246
DpnI GATC 2 cut(s) 291, 308
DpnII GATC 2 cut(s) 289, 306
Eam1104I CTCTTC 2 cut(s) 41, 47
EarI CTCTTC 2 cut(s) 41, 47
Eco130I CCWWGG 3 cut(s) 72, 159, 271
Eco47I GGWCC 1 cut(s) 190
EcoT14I CCWWGG 3 cut(s) 72, 159, 271
ErhI CCWWGG 3 cut(s) 72, 159, 271
FaeI CATG 1 cut(s) 275
FaiI YATR 2 cut(s) 231, 273
FalI AAGNNNNNCTT 1 cut(s) 293
FatI CATG 1 cut(s) 271
Fnu4HI GCNGC 1 cut(s) 99
Fsp4HI GCNGC 1 cut(s) 99
GluI GCNGC 1 cut(s) 99
HaeIII GGCC 1 cut(s) 71
HapII CCGG 1 cut(s) 193
HgaI GACGC 1 cut(s) 91
Hin1II CATG 1 cut(s) 275
HindIII AAGCTT 2 cut(s) 128, 248
HinfI GANTC 3 cut(s) 32, 225, 242
HpaII CCGG 1 cut(s) 193
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 1 cut(s) 156
Hpy188I TCNGA 1 cut(s) 31
Hpy8I GTNNAC 1 cut(s) 156
HpyAV CCTTC 1 cut(s) 169
HpyCH4III ACNGT 1 cut(s) 153
HpyCH4V TGCA 1 cut(s) 313
HpyF3I CTNAG 2 cut(s) 117, 246
Hsp92II CATG 1 cut(s) 275
Kzo9I GATC 2 cut(s) 289, 306
LpnPI CCDG 3 cut(s) 71, 206, 252
MalI GATC 2 cut(s) 291, 308
MboI GATC 2 cut(s) 289, 306
MboII GAAGA 3 cut(s) 58, 64, 316
MflI RGATCY 1 cut(s) 306
MlyI GAGTC 2 cut(s) 41, 251
MnlI CCTC 3 cut(s) 42, 55, 215
MspI CCGG 1 cut(s) 193
Mva1269I GAATGC 1 cut(s) 118
MvnI CGCG 1 cut(s) 170
NcoI CCATGG 1 cut(s) 271
NdeII GATC 2 cut(s) 289, 306
NlaIII CATG 1 cut(s) 275
PctI GAATGC 1 cut(s) 118
PfeI GAWTC 1 cut(s) 225
PkrI GCNGC 1 cut(s) 100
PleI GAGTC 2 cut(s) 40, 250
PpsI GAGTC 2 cut(s) 40, 250
PspPI GGNCC 2 cut(s) 69, 190
PsuI RGATCY 1 cut(s) 306
RsaI GTAC 2 cut(s) 155, 167
RsaNI GTAC 2 cut(s) 154, 166
SatI GCNGC 1 cut(s) 99
Sau3AI GATC 2 cut(s) 289, 306
Sau96I GGNCC 2 cut(s) 69, 190
SchI GAGTC 2 cut(s) 41, 251
SetI ASST 3 cut(s) 21, 132, 252
SinI GGWCC 1 cut(s) 190
SsiI CCGC 2 cut(s) 99, 170
StyI CCWWGG 3 cut(s) 72, 159, 271
TaaI ACNGT 1 cut(s) 153
TatI WGTACW 1 cut(s) 153
TauI GCSGC 1 cut(s) 101
TfiI GAWTC 1 cut(s) 225
VpaK11BI GGWCC 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.